CN101586127A - Method for producing 2,3-butanediol clean fuel through liquid-state fermentation of straw resources - Google Patents
Method for producing 2,3-butanediol clean fuel through liquid-state fermentation of straw resources Download PDFInfo
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- CN101586127A CN101586127A CNA2009100121683A CN200910012168A CN101586127A CN 101586127 A CN101586127 A CN 101586127A CN A2009100121683 A CNA2009100121683 A CN A2009100121683A CN 200910012168 A CN200910012168 A CN 200910012168A CN 101586127 A CN101586127 A CN 101586127A
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- state fermentation
- butyleneglycol
- bacterium
- liquid
- obtains
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- 238000000855 fermentation Methods 0.000 title claims abstract description 33
- 230000004151 fermentation Effects 0.000 title claims abstract description 33
- 239000000446 fuel Substances 0.000 title claims abstract description 26
- 239000010902 straw Substances 0.000 title claims abstract description 18
- OWBTYPJTUOEWEK-UHFFFAOYSA-N butane-2,3-diol Chemical compound CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 title abstract description 19
- 238000004519 manufacturing process Methods 0.000 title abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 30
- 239000001913 cellulose Substances 0.000 claims abstract description 19
- 229920002678 cellulose Polymers 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 43
- 241000894006 Bacteria Species 0.000 claims description 22
- 241000209094 Oryza Species 0.000 claims description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 12
- 235000009566 rice Nutrition 0.000 claims description 12
- 238000004821 distillation Methods 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 241000588748 Klebsiella Species 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 8
- 108010080698 Peptones Proteins 0.000 claims description 8
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 8
- 235000019319 peptone Nutrition 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims 2
- 229940058015 1,3-butylene glycol Drugs 0.000 claims 1
- 235000019437 butane-1,3-diol Nutrition 0.000 claims 1
- 238000010411 cooking Methods 0.000 abstract description 3
- 230000009977 dual effect Effects 0.000 abstract 1
- 108010059892 Cellulase Proteins 0.000 description 6
- 229940106157 cellulase Drugs 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 5
- 229920002488 Hemicellulose Polymers 0.000 description 4
- 239000002699 waste material Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000010808 liquid waste Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000003113 alkalizing effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- ACGUYXCXAPNIKK-UHFFFAOYSA-N hexachlorophene Chemical compound OC1=C(Cl)C=C(Cl)C(Cl)=C1CC1=C(O)C(Cl)=CC(Cl)=C1Cl ACGUYXCXAPNIKK-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for producing 2,3-butanediol clean fuel through liquid-state fermentation of straw resources. The method is characterized by comprising the following: 1) a step of preparing a raw material, which is to grind straw cellulose raw material obtained through alkalization in the paper industry, by a liquefaction method or through alkalization cooking for standby application, wherein cellulose accounts for 60 to 71 percent; 2) a step of performing enzymolysis, which is to add a cellulose complex enzyme preparation (Cellulose Multi-enzyme) to the raw material according to the enzyme addition amount between 2 to 5 percent, add water 5 to 7 times the weight of the raw material and perform enzymolysis for 3 to 5 days with pH between 5.5 and 7.0 at a temperature between 35 and 45 DEG C; 3) a step of performing liquid-state fermentation; and 4) a step of distilling, which is to distill under reduced pressure so as to obtain 2,3-butanediol. The production of the 2,3-butanediol fuel has the characteristic of low production cost, and has dual effect of developing resources and protecting environment.
Description
Technical field
The present invention relates to a kind of biological enzyme and produce 2 in the rice straw resource, the utilisation technology in the 3-butyleneglycol clean fuel belongs to the biomass energy field.
Technical background
2,3-butyleneglycol fuel can be inexhausted as renewable energy source, and can not cause Greenhouse effect.Microbial fermentation sugar can produce 2,3-butyleneglycol fuel.In industrial production, be used for fermentation at present and produce 2, if the microbial host bacterium-klebsiella (Klebsiella) of 3-butyleneglycol or Bacillussubtilis genus (Bacillus) etc.The biomass that contain glucide that comprise stalk all may be as 2, the raw material of 3-butyleneglycol fermentation, and the utilization of macromolecular substance needs the degraded of process enzyme earlier.Biology 2,3-butyleneglycol are as the surrogate of oil, and its industrial chain is also continuing extension.There is development Mierocrystalline cellulose system 2 in China, the favourable condition of 3-butyleneglycol, every year, only rice straw just had more than 300,000,000 ton (dry weight), and China's grain resource is not abundant, therefore agriculture and forestry organic waste material is converted into 2,3-butyleneglycol fuel, the formation industrialization utilizes, be fit to very much the national conditions of China, also be very favourable from the energy security angle, and can eliminate the environmental problem that causes by crop straw burning.
Existing stalk (rice straw, maize straw, wheat stalk) resource produces 2, and no matter the cleaning technique of 3-butyleneglycol fuel as being raw material with full stalk, adopt acid hydrolyzation or enzymolysis process to produce 2,3-butyleneglycol clean fuel; Wherein a large amount of hemicelluloses, xylogen and ash content remain in the fermented liquid, bring the difficult problem of follow-up liquid waste disposal to be difficult to so far solve.
Summary of the invention
The objective of the invention is with paper industry after alkalizing or the Mierocrystalline cellulose that obtains of liquefaction process be main raw material, handle through biotechnology, produce biological cheaply 2,3-butyleneglycol fuel.
The present invention relates to a kind of rice straw resource 2, the cleaning technique of 3-butyleneglycol fuel production are as with full rice straw being raw material employing acid hydrolyzation or enzymolysis process production 2,3-butyleneglycol clean fuel; Wherein a large amount of hemicelluloses, xylogen and ash content remain in the fermented liquid, bring the difficult problem of follow-up liquid waste disposal to be difficult to so far solve.And the present invention is characterized in that it selects straw for use is raw material, by alkaline process or liquefaction process wherein a large amount of hemicellulose, xylogen and ash separation gone out, and the Mierocrystalline cellulose (hexosan) that obtains is converted into glucose, insert bacterium-Klebsiella (Klebsiella) or Bacillussubtilis genus (Bacillus) and carry out 2, the 3-butyleneglycol fermentation is produced, use grass to replace provisions, turn waste into wealth, reduced by 2,3-butyleneglycol production cost; Have only a spot of residual hemicellulose, lignin and ash content in the waste liquid, even the raw water hydrolysis and fermentation is incomplete, solid-liquid separation is easy in the waste liquid, and is also recycling, therefore reduced environmental pollution.Per kilogram rice straw cellulosic material produces liquid glucose 0.6kg, and 2,3 butyleneglycol fuel 0.2-0.30kg are extracted in the fermentation ends underpressure distillation.This 2, the production of 3-butyleneglycol fuel has the low characteristics of production cost, playing exploit natural resources the protection environment double effects.
The objective of the invention is to realize by following technical proposal:
Rice straw liquid state fermentation 2, the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that may further comprise the steps:
1) cellulosic material or the cellulosic material in the stalk that the alkalization boiling obtains in the raw material stalk preparing to obtain with the cellulosic material in the stalk that alkalization obtains, liquefaction process in paper industry, wherein Mierocrystalline cellulose 60～71%, pulverize standby;
2) enzymolysis is got above-mentioned raw materials and is added cellulose complex enzyme preparation (CelluloseMulti-enzyme) according to 2～5% enzyme concentration, presses 5～7 times of raw material weight at water addition ratio, and enzymolysis is 3～5 days under the condition of pH5.5～7.0,35～45 ℃;
3) liquid state fermentation;
4) the distillation underpressure distillation obtains 2, the 3-butyleneglycol.
Described in paper industry the Mierocrystalline cellulose of the cellulosic material in the stalk that alkalization obtains be 60～71%.
Described cellulosic material in the stalk that liquefaction process obtains is 60～68%.
The inoculum size adding acid-producing Klebsiella bacterium that described liquid state fermentation process specifically is preferably by 1～5% belongs to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% added water to 100ml (W/V), 30～40 ℃ of heat-preservation fermentations 3～5 days.
Embodiment
Embodiment 1
1 kilogram of the Mierocrystalline cellulose that paper industry obtains after alkalization.Ratio in 2% adds cellulase, pH value 6.5, and 5.5,36 ℃ of enzymolysis of water addition ratio are about 3 days.Add bacterium bacterium liquid by 2% inoculum size then, 35 ℃ of heat-preservation fermentations 3 days, liquid state fermentation process inoculation acid-producing Klebsiella bacterium belonged to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.24kg through distillation.
Embodiment 2
1 kilogram of the Mierocrystalline cellulose that obtains through alkaline cooking.Ratio in 3% adds cellulase, and at pH value 6.0,6.0,38 ℃ of enzymolysis of water addition ratio are about 4 days.Add bacterium bacterium liquid by 3% inoculum size then, 36 ℃ of heat-preservation fermentations 3 days, liquid state fermentation process inoculation acid-producing Klebsiella bacterium belonged to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.26kg through underpressure distillation.
Embodiment 3
1 kilogram of the Mierocrystalline cellulose that liquefaction process obtains.Ratio in 3% adds cellulase, at water addition ratio 6.0, and 6.5,40 ℃ of enzymolysis of pH value 3 days.Add bacterium bacterium liquid by 2% inoculum size then, 36 ℃ of heat-preservation fermentations 3 days, liquid state fermentation process inoculation acid-producing Klebsiella bacterium belongs to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.27kg through underpressure distillation.
Embodiment 4
1 kilogram of the Mierocrystalline cellulose that liquefaction process obtains.Ratio in 4% adds cellulase, at water addition ratio 6.5, and 6.5,42 ℃ of enzymolysis of pH value 4 days.Add bacterium bacterium liquid by 3% inoculum size then, 38 ℃ of heat-preservation fermentations 3 days, liquid state fermentation process inoculation acid-producing Klebsiella bacterium belongs to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.28kg through underpressure distillation.
Embodiment 5
1 kilogram of the Mierocrystalline cellulose that paper industry obtains after alkalization.Ratio in 5% adds cellulase, pH value 7.0, and 7.0,45 ℃ of enzymolysis of water addition ratio are about 5 days.Add bacterium bacterium liquid by 5% inoculum size then, 40 ℃ of heat-preservation fermentations 5 days, liquid state fermentation process inoculation acid-producing Klebsiella bacterium belonged to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.27kg through distillation.
Embodiment 6
1 kilogram of the Mierocrystalline cellulose that obtains through alkaline cooking.Ratio in 3% adds cellulase, and at pH value 5.5,5.0,35 ℃ of enzymolysis of water addition ratio are about 4 days.Add bacterium bacterium liquid by 1% inoculum size then, 30 ℃ of heat-preservation fermentations 4 days, liquid state fermentation process inoculation acid-producing Klebsiella bacterium belonged to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.24kg through underpressure distillation.
Claims (4)
1, rice straw liquid state fermentation 2, and the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that may further comprise the steps:
1) cellulosic material or the cellulosic material in the stalk that the alkalization boiling obtains in the raw material stalk preparing to obtain with the cellulosic material in the stalk that alkalization obtains, liquefaction process in paper industry, wherein Mierocrystalline cellulose 60～71%, pulverize standby;
2) enzymolysis is got above-mentioned raw materials and is added cellulose complex enzyme preparation (CelluloseMulti-enzyme) according to 2～5% enzyme concentration, presses 5～7 times of raw material weight at water addition ratio, and enzymolysis is 3～5 days under the condition of pH5.5～7.0,35～45 ℃;
3) liquid state fermentation;
4) the distillation underpressure distillation obtains 2, the 3-butyleneglycol.
2, the method for clean fuel is produced in rice straw liquid state fermentation 2 as claimed in claim 1,3-butyleneglycol, it is characterized in that described in paper industry the Mierocrystalline cellulose of the cellulosic material in the stalk that alkalization obtains be 60～71%.
3, rice straw liquid state fermentation 2 as claimed in claim 1, the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that described cellulosic material in the stalk that liquefaction process obtains is 60～68%.
4, as any described rice straw liquid state fermentation 2 in the claim 1～3, the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that the inoculum size adding acid-producing Klebsiella bacterium that described liquid state fermentation is specially by 1～5% belongs to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% added water to 100ml (W/V), 30～40 ℃ of heat-preservation fermentations 3～5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100121683A CN101586127A (en) | 2009-06-22 | 2009-06-22 | Method for producing 2,3-butanediol clean fuel through liquid-state fermentation of straw resources |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100121683A CN101586127A (en) | 2009-06-22 | 2009-06-22 | Method for producing 2,3-butanediol clean fuel through liquid-state fermentation of straw resources |
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| CN101586127A true CN101586127A (en) | 2009-11-25 |
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| CNA2009100121683A Pending CN101586127A (en) | 2009-06-22 | 2009-06-22 | Method for producing 2,3-butanediol clean fuel through liquid-state fermentation of straw resources |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580855B (en) * | 2009-06-22 | 2012-05-23 | 大连工业大学 | Clean technology for producing 2,3-butanediol by maize straw resource |
| CN102864175A (en) * | 2012-10-01 | 2013-01-09 | 柳州市京阳节能科技研发有限公司 | High-efficiency liquid-state alcohol preparation method by wild straw leaves |
-
2009
- 2009-06-22 CN CNA2009100121683A patent/CN101586127A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580855B (en) * | 2009-06-22 | 2012-05-23 | 大连工业大学 | Clean technology for producing 2,3-butanediol by maize straw resource |
| CN102864175A (en) * | 2012-10-01 | 2013-01-09 | 柳州市京阳节能科技研发有限公司 | High-efficiency liquid-state alcohol preparation method by wild straw leaves |
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Application publication date: 20091125 |