CN108165585A - Alcohol fermentation method - Google Patents
Alcohol fermentation method Download PDFInfo
- Publication number
- CN108165585A CN108165585A CN201810130336.8A CN201810130336A CN108165585A CN 108165585 A CN108165585 A CN 108165585A CN 201810130336 A CN201810130336 A CN 201810130336A CN 108165585 A CN108165585 A CN 108165585A
- Authority
- CN
- China
- Prior art keywords
- solution
- fermentation method
- alcohol fermentation
- saccharification
- propionic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The present invention provides a kind of alcohol fermentation methods, include the following steps：A, slurrying obtains starchy material, and the starchy material is crushed and is mixed to form starch solution with spice water；B, it liquefies, α-amylase is added in the starch solution and is prepared into liquefied solution, c, saccharification, addition carbohydrase is prepared into saccharified solution in the liquefied solution；D, it ferments, propionic acid is added in the saccharified solution, inoculate seed liquor and carry out alcoholic fermentation.Alcohol fermentation method of the present invention by adding propionic acid in the starch solution after saccharification, can be such that the conversion ratio of starch in alcoholic fermentation process improves to more than 90%, and the propionic acid added is at low cost, be conducive to improve the yield and its profit of alcohol.
Description
Technical field
The present invention relates to alcoholic fermentation industrial technical field, more particularly to a kind of alcohol fermentation method.
Background technology
Since Global Oil storage level is reduced and fossil energy use causes serious air pollution, in world wide
Many countries are all in positive Developing Biomass fuel market.The wherein more traditional fossil energy of fuel alcohol has cleaning can be again
The advantages that raw, can in the world obtain big directly as motor vehicle fuel or added to use in gasoline (ethanol petrol)
Power develops.
Fuel alcohol is mainly produced using fermentation method, and general production process is：1) raw material is pre-processed, and acquisition can be sent out
Ferment sugar；2) saccharomyces cerevisiae generates alcohol using fermentable sugars；3) zymotic fluid obtains finished product combustion through processes such as distillation-rectifying-dehydrations
Cooking wine essence.Since in alcoholic fermentation process, fermentable sugars, using other than generating major product alcohol, can also be synthesized in addition to by saccharomyces cerevisiae
In addition eucaryotic cell structure substance and other metabolic by-products can also remain a part of sugar, it is complete to be unable to saccharomyces cerevisiae in zymotic fluid
Complete utilization, the Starch Conversion rate that this results in fermentation process is insufficient.
Invention content
In view of this, the present invention is directed to propose a kind of alcohol fermentation method, to improve Starch Conversion in alcoholic fermentation process
Rate.
In order to achieve the above objectives, the technical proposal of the invention is realized in this way：
A kind of alcohol fermentation method, includes the following steps：
A, slurrying obtains starchy material, and starch solution is mixed to form with spice water after the starchy material is crushed；
B, it liquefies, α-amylase is added in the starch solution and is prepared into liquefied solution；
C, it is saccharified, carbohydrase is added in the liquefied solution and is prepared into saccharified solution,
D, it ferments, propionic acid is added in the saccharified solution, inoculate seed liquor and carry out alcoholic fermentation.
Further, a concentration of 200~700mg/L of the propionic acid.
Further, the starchy material and the spice water are with 1:2~5 material-water ratio is mixed.
Further, the seed liquor is using saccharomyces cerevisiae as fermenting microbe, and thalline quantity is 1.9 × 106~2.1 ×
106The mature seed liquid of CFU/mL.
Further, two parts are divided by the saccharified solution that step c is prepared, a part is prepared to described
Seed liquor, another part are used in step d.
Further, the α-amylase is the alpha-amylase of 15~20u/g.
Further, the liquefied condensing temperature is 90~95 DEG C, and liquefying time is 90~140 minutes.
Further, the carbohydrase is the carbohydrase of 120~160u/g.
Further, the saccharification temperature of the saccharification is 45~60 DEG C, and saccharificatinn period is 20~60 minutes.
Further, the starchy material is at least one of cassava, corn, wheat.
Relative to the prior art, the present invention has the advantage that：
(1) alcohol fermentation method of the present invention by adding propionic acid in the starch solution after saccharification, can make alcohol
The conversion ratio of starch is improved to more than 90% in fermentation process, and the propionic acid added is at low cost, is conducive to improve the yield of alcohol
And its profit.
(2) in pulping process, the mixed proportion of setting starchy material and spice water is 1:2~5, this ratio can make
It obtains different starchiness and the starch solution of the formation of spice water easily liquefies.
(2) concentration of propionic acid is arranged on 200~700mg/L, can be to being carried out using saccharified solution made of different starchiness
Starch Conversion.
(3) starch solution can be changed into the liquefied solution being easily saccharified by liquefaction process, meanwhile, when condensing temperature is with liquefying
Between setting, the activity of alpha-amylase can be effectively ensured, and then promote the liquefaction of starchiness solution.
(4) liquefied solution can be changed into the carbohydrate easily to ferment by saccharifying, meanwhile, saccharification temperature and saccharificatinn period
Setting can effectively ensure the activity of carbohydrase, and then promote the saccharification of liquefied solution.
(5) seed liquor that a part for saccharified solution is prepared into, available for alcoholic fermentation；Another part adds in propionic acid and can carry
High Starch Conversion rate.
(6) alcoholic fermentation is carried out using starchy material, it is convenient to obtain, and is easily worked processing.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
Embodiment one
A kind of alcohol fermentation method, includes the following steps：
A, slurrying obtains starchy material, and is mixed to form starch solution with spice water after starchy material is crushed；
B, it liquefies, α-amylase is added in starch solution and is prepared into liquefied solution；
C, it is saccharified, carbohydrase is added in liquefied solution and is prepared into saccharified solution；
D, it ferments, propionic acid is added in saccharified solution, inoculate seed liquor and carry out alcoholic fermentation.
Wherein：The starchy material of step a is at least one of cassava, corn, wheat, using the starchiness after crushing
Raw material carries out alcoholic fermentation, and it is convenient to obtain, and easy pulverization process；Setting starchy material and spice water mixed proportion be
1:2~5, this ratio can cause different starchiness and the starch solution of the formation of spice water easily to liquefy.
Starch solution can be changed into the liquefied solution being easily saccharified, the liquefaction that the present embodiment uses by step b liquefaction process
Mould is the Thermostable α-Amylase of 15~20u/g, and it is 90~95 DEG C to carry out liquefied condensing temperature, and liquefying time is 90~140
The activity that alpha-amylase can be effectively ensured is arranged such in minute, promotes the liquefaction of starchiness solution.
Liquefied solution can be changed into the carbohydrate easily to ferment by step c saccharifyings, and wherein saccharifying mould is 120~160u/g
Carbohydrase, and set saccharification temperature be 45~60 DEG C, saccharificatinn period be 20~60 minutes, can effectively ensure to be saccharified in this way
The activity of enzyme promotes the saccharification of liquefied solution.
In addition, the saccharified solution in step c is divided into two parts, the seed liquor that a part is prepared into, another part adds in
Propionic acid can improve Starch Conversion rate.Wherein, seed liquor be using saccharomyces cerevisiae as fermenting microbe, thalline quantity for 1.9 × 106~
The mature seed liquid of 2.1 × 106CFU/mL carries out alcoholic fermentation available for the saccharified solution after saccharification.
A concentration of 200~700mg/L of propionic acid is added in step d in saccharified solution, this concentration range can be applicable in
In using different amylaceous saccharified solutions progress Starch Conversions；Material after fermentation is obtained into using distillation and rectifying
It samples wine essence.
Alcohol fermentation method of the present invention by adding propionic acid in the starch solution after saccharification, can send out alcohol
The conversion ratio of starch is improved to more than 90% during ferment, and the propionic acid added is at low cost, be conducive to improve alcohol yield and
Its profit.
Based on as above thought, a kind of embodiment of the invention be a kind of alcohol fermentation method, specific alcoholic fermentation work
Skill step is as follows：
A, tapioca starch is mixed with spice water, material-water ratio 1:2~5, add in the alphalise starch that α-amylase is 15~20u/g
Enzyme is mixing uniformly to form tapioca starch solution, and tapioca starch solution is heated to 90~95 DEG C, and maintain 90~140 minutes,
Wherein material-water ratio is 1:2, the alpha-amylase of 15u/g is added in, heating temperature is 95 DEG C, holds time and is set as 90 points
Clock.
B, the tapioca starch solution in step a is cooled to 40~60 DEG C, adds the carbohydrase of 120~160u/g, stirred
It is kept the temperature after uniformly, carries out saccharification 20~60 minutes, to form the tapioca starch solution that is saccharified；40 DEG C are cooled in the embodiment, is added in
The carbohydrase of 120u/g, and be saccharified 60 minutes
50 DEG C are cooled in setting steps b, adds in the carbohydrase of 120u/g, saccharificatinn period 60 minutes, to realize fully sugar
Change.
C, step b saccharification tapioca starch solution is cooled to 30 DEG C, and takes wherein 15% saccharification tapioca starch solution for making
Standby yeast starter liquid adds in the propionic acid of final concentration of 200mg/L in remaining saccharification tapioca starch solution, adds mature seed
Start fermentation after liquid.
D, after fermentation, alcohol is obtained through distilling the processes such as rectifying.
In above step, tapioca starch solution is heated and kept the temperature, Thermostable α-Amylase can be made to be in high activity state, it is molten
Liquid preferably liquefies；Carbohydrase is added in after cooling down to tapioca starch solution and is kept the temperature, the activity that can make carbohydrase is high, promotes tapioca starch
Solution is better, and saccharification tapioca starch solution cools down again can be convenient for the addition for preparing seed liquor and propionic acid.
Not add propionic acid as blank control, the Starch Conversion rate of addition propionic acid is 89.6% in the present embodiment, blank
The Starch Conversion rate of control is 88.1%, and Starch Conversion rate improves 1.5% in contrast.
Embodiment two
A kind of alcohol fermentation method, specific technology of alcohol step are as follows：
A, corn flour is mixed with spice water, material-water ratio 1:2~5, it is 15~20u/g to add in high temperature resistant and add in α-amylase
Alpha-amylase, be mixing uniformly to form corn flour solution, by corn flour solution be heated to 90~95 DEG C and maintain 90~140 points
Clock；
It is 1 to be provided with material-water ratio:3, add in the alpha-amylase of 20u/g, and heating temperature is 90 DEG C, hold time for
120 minutes.
B, the corn flour solution in step a is cooled to 40~60 DEG C, adds the carbohydrase of 120~160u/g, stirred
It is kept the temperature after uniformly, carries out saccharification 20~60 minutes, form saccharification corn flour solution；
60 DEG C are cooled in setting steps b, adds in the carbohydrase of 160u/g, saccharificatinn period 40 minutes, to realize fully sugar
Change.
C, step b saccharification corn flour solution is cooled to 30 DEG C, and takes wherein 10% saccharification corn flour solution for making
Standby yeast starter liquid adds in the propionic acid of final concentration of 300mg/L in remaining saccharification corn flour solution, adds mature seed
Start fermentation after liquid.
D, after fermentation, alcohol is obtained through distilling the processes such as rectifying.
In the present embodiment, not add propionic acid as blank control, the Starch Conversion rate for adding propionic acid is 90.8%, blank
The Starch Conversion rate of control is 88.9%, and 1.9% is improved by adding propionic acid Starch Conversion rate.
Embodiment three
A kind of alcohol fermentation method, specific technology of alcohol step are as follows：
A, tapioca starch, corn flour and wheat flour are mixed with spice water, material-water ratio 1:2~5, it adds in high temperature resistant and adds in liquid
Change the alpha-amylase that enzyme is 15~20u/g, be mixing uniformly to form mixed powder solution, mixed powder solution is heated to 90~95 DEG C
And it maintains 90~140 minutes；
It is 1 that material-water ratio is set in above step:5, the alpha-amylase of 18u/g is added in, and heating temperature is 92 DEG C, during maintenance
Between be 140 minutes.
B, the mixed powder solution in step a is cooled to 40~60 DEG C, adds the carbohydrase of 120~160u/g, stirred
It is kept the temperature after uniformly, carries out saccharification 20~60 minutes, form saccharification mixed powder solution；
Wherein, 40 DEG C are cooled in setting steps b, adds in the carbohydrase of 140u/g, saccharificatinn period 20 minutes is filled with realizing
Divide saccharification.
C, step b saccharification mixed powder solution is cooled to 30 DEG C, and takes wherein 20% saccharification mixed powder solution for making
Standby yeast starter liquid adds in the propionic acid of final concentration of 700mg/L in remaining saccharification mixed powder solution, adds mature seed
Start fermentation after liquid.
D, after fermentation, alcohol is obtained through distilling the processes such as rectifying.
In the present embodiment, not add propionic acid as blank control, the Starch Conversion rate for adding propionic acid is 90.2%, blank
The Starch Conversion rate of control is 88.3%, and 1.9% is improved by adding propionic acid Starch Conversion rate.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (10)
1. a kind of alcohol fermentation method, it is characterised in that include the following steps：
A, slurrying obtains starchy material, and starch solution is mixed to form with spice water after the starchy material is crushed；
B, it liquefies, α-amylase is added in the starch solution and is prepared into liquefied solution；
C, it is saccharified, carbohydrase is added in the liquefied solution and is prepared into saccharified solution；
D, it ferments, propionic acid is added in the saccharified solution, inoculate seed liquor and carry out alcoholic fermentation.
2. alcohol fermentation method according to claim 1, it is characterised in that：A concentration of 200~700mg/ of the propionic acid
L。
3. alcohol fermentation method according to claim 1, it is characterised in that：The starchy material and the spice water with
1:2~5 material-water ratio is mixed.
4. alcohol fermentation method according to claim 1, it is characterised in that：The seed liquor is using saccharomyces cerevisiae as fermentation
Strain, thalline quantity are 1.9 × 106~2.1 × 106The mature seed liquid of CFU/mL.
5. alcohol fermentation method according to claim 1, it is characterised in that：The saccharification being prepared by step c is molten
Liquid is divided into two parts, and a part is prepared to the seed liquor, and another part is used in step d.
6. alcohol fermentation method according to claim 1, it is characterised in that：The α-amylase is α-shallow lake of 15~20u/g
Powder enzyme.
7. alcohol fermentation method according to claim 1, it is characterised in that：The liquefied condensing temperature is 90~95
DEG C, liquefying time is 90~140 minutes.
8. alcohol fermentation method according to claim 1, it is characterised in that：The carbohydrase is the sugar of 120~160u/g
Change enzyme.
9. alcohol fermentation method according to claim 1, it is characterised in that：The saccharification temperature of the saccharification is 45~60
DEG C, saccharificatinn period is 20~60 minutes.
10. according to the alcohol fermentation method described in any one of claim 1-9, it is characterised in that：The starchy material is
At least one of cassava, corn, wheat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130336.8A CN108165585A (en) | 2018-02-08 | 2018-02-08 | Alcohol fermentation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130336.8A CN108165585A (en) | 2018-02-08 | 2018-02-08 | Alcohol fermentation method |
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| CN108165585A true CN108165585A (en) | 2018-06-15 |
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ID=62513835
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|---|---|---|---|
| CN201810130336.8A Pending CN108165585A (en) | 2018-02-08 | 2018-02-08 | Alcohol fermentation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949863A (en) * | 2018-07-12 | 2018-12-07 | 安徽省碧绿春生物科技有限公司 | A method of absolute alcohol is produced using corn stover |
| CN109536539A (en) * | 2018-11-20 | 2019-03-29 | 江苏金茂源生物化工有限责任公司 | Ethyl alcohol preparation method |
| CN113025450A (en) * | 2019-12-23 | 2021-06-25 | 纪忠孝 | Edible alcohol and preparation method thereof |
| CN113444749A (en) * | 2020-03-27 | 2021-09-28 | 中国石油天然气股份有限公司 | Preparation method of bioethanol |
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| US20040187863A1 (en) * | 2003-03-25 | 2004-09-30 | Langhauser Associates Inc. | Biomilling and grain fractionation |
| WO2005089514A2 (en) * | 2004-03-19 | 2005-09-29 | Novozymes North America, Inc | Liquefaction processes |
| CN101880692A (en) * | 2010-07-06 | 2010-11-10 | 江南大学 | Alcohol fermentation method capable of improving raw material utilization rate |
| CN101974567A (en) * | 2010-09-20 | 2011-02-16 | 江南大学 | Method for ecologically producing alcohol by taking tuberous crop as raw material |
-
2018
- 2018-02-08 CN CN201810130336.8A patent/CN108165585A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040187863A1 (en) * | 2003-03-25 | 2004-09-30 | Langhauser Associates Inc. | Biomilling and grain fractionation |
| WO2005089514A2 (en) * | 2004-03-19 | 2005-09-29 | Novozymes North America, Inc | Liquefaction processes |
| CN101880692A (en) * | 2010-07-06 | 2010-11-10 | 江南大学 | Alcohol fermentation method capable of improving raw material utilization rate |
| CN101974567A (en) * | 2010-09-20 | 2011-02-16 | 江南大学 | Method for ecologically producing alcohol by taking tuberous crop as raw material |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949863A (en) * | 2018-07-12 | 2018-12-07 | 安徽省碧绿春生物科技有限公司 | A method of absolute alcohol is produced using corn stover |
| CN109536539A (en) * | 2018-11-20 | 2019-03-29 | 江苏金茂源生物化工有限责任公司 | Ethyl alcohol preparation method |
| CN113025450A (en) * | 2019-12-23 | 2021-06-25 | 纪忠孝 | Edible alcohol and preparation method thereof |
| CN113444749A (en) * | 2020-03-27 | 2021-09-28 | 中国石油天然气股份有限公司 | Preparation method of bioethanol |
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Application publication date: 20180615 |