CN107828827B - Method for increasing conversion rate of alcohol fermentation starch by adding acetic acid - Google Patents
Method for increasing conversion rate of alcohol fermentation starch by adding acetic acid Download PDFInfo
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- CN107828827B CN107828827B CN201711367979.6A CN201711367979A CN107828827B CN 107828827 B CN107828827 B CN 107828827B CN 201711367979 A CN201711367979 A CN 201711367979A CN 107828827 B CN107828827 B CN 107828827B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H6/00—Methods for increasing the alcohol content of fermented solutions or alcoholic beverages
- C12H6/02—Methods for increasing the alcohol content of fermented solutions or alcoholic beverages by distillation
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses a method for improving the conversion rate of starch in alcohol fermentation by adding acetic acid, belonging to the field of alcohol fermentation industry. According to the invention, acetic acid is added into the fermentation system containing the starchy raw material to perform alcohol fermentation, so that the purposes of improving starch conversion rate and improving alcohol yield are achieved. The method of the invention can improve the conversion rate of the starch to more than 90 percent. Compared with the prior art, the method has the greatest advantages that the starch conversion rate in the alcohol fermentation process can be improved, the generation of the byproduct glycerol is reduced, and the added acetic acid cost is far lower than the profit generated by producing more alcohol.
Description
Technical Field
The invention relates to a method for improving the conversion rate of starch by alcohol fermentation by adding acetic acid, belonging to the field of alcohol fermentation industry.
Background
Due to the decrease in global oil reserves and the severe air pollution caused by the use of fossil energy, the biomass fuel market is actively developing in many countries worldwide including the united states, brazil, european union, china, japan, canada, india, etc. Compared with the traditional fossil energy, the fuel alcohol has the advantages of cleanness, renewability and the like, and can be directly used as automobile fuel or added into gasoline (ethanol gasoline) for use. The ethanol is added into the gasoline to improve the combustion performance, the power performance and the environmental protection performance of the gasoline, mainly shows that the oxygen content of the gasoline is increased, the octane number and the anti-explosion index are improved, the aromatic hydrocarbon content of the gasoline is reduced, and the contents of hydrocarbon, NOx and CO in the automobile exhaust are reduced. In addition, the development of the fuel alcohol industry can drive the coordinated development of industries such as manufacturing industry and agriculture, and the like, and drive the economic growth. Thus, fuel alcohol processes have been vigorously developed on a global scale. Over the past few decades, global fuel alcohol production has increased dramatically, from less than 80 million tons (1975) to 7700 million tons in 2015. The fuel alcohol industry in China starts late, but under the promotion of national policies, the production scale is continuously enlarged, the yield is rapidly increased, and the yield is leaping the third place in the world at present (about 200 ten thousand tons in 2015).
The fuel alcohol is mainly produced by a fermentation method, and the general production process comprises the following steps: 1) pretreating raw materials to obtain fermentable sugar; 2) the saccharomyces cerevisiae utilizes fermentable sugar to generate alcohol; 3) the fermentation liquor is distilled, rectified and dehydrated to obtain the finished product of fuel alcohol. In the alcohol fermentation process, besides being utilized by the saccharomyces cerevisiae to generate main product alcohol, the fermentable sugar also synthesizes cell structural substances and other metabolic byproducts, and in addition, a part of sugar remains in the fermentation liquor, so that the saccharomyces cerevisiae cannot be completely utilized. This results in less than 100% starch conversion during fermentation. If measures can be taken to make more sugar available for alcohol synthesis, i.e. to increase the starch conversion, the profit of the alcohol production enterprise can be significantly increased. Taking a fuel alcohol enterprise producing 10 ten thousand tons per year as an example, even if the starch conversion rate is improved by 1%, the profit can be increased by about 490 thousand per year (based on 4900 yuan/ton of fuel alcohol), and the profit is very considerable for the alcohol industry with lower profit margin.
Disclosure of Invention
The invention aims to provide a method for improving starch conversion rate in alcohol fermentation, which is to add acetic acid into a fermentation system containing starchy materials so as to improve the starch conversion rate.
In one embodiment of the present invention, acetic acid is added to a saccharified solution after saccharification to a final concentration of 300 to 900mg/L, and then a seed solution is inoculated for fermentation.
In one embodiment of the invention, the starchy material is a mixed material of one or more of cassava, corn, wheat and the like.
The second purpose of the invention is to provide an alcohol fermentation method, which comprises the following process steps:
(1) mixing the crushed starchy raw materials with stirring water, adding liquefying enzyme, and heating for liquefaction;
(2) cooling after liquefaction, and adding saccharifying enzyme for saccharification;
(3) cooling after saccharification is finished, wherein the cooled saccharification liquid is divided into two parts, one part is directly used for preparing seed liquid, and the other part is added with acetic acid; inoculating the prepared seed liquid into the saccharification liquid added with acetic acid, and starting alcoholic fermentation;
(4) after fermentation, alcohol is obtained through distillation.
In one embodiment of the invention, the starchy raw material and the stirring water are mixed in a ratio of 1: 2-5.
In one embodiment of the invention, the liquefying enzyme includes, but is not limited to, a high temperature resistant alpha-amylase.
In one embodiment of the invention, the liquefaction is carried out by adding high temperature resistant alpha-amylase into 15-20U/g starch, and reacting at 90-95 ℃ for 90-140 min.
In one embodiment of the present invention, the saccharification is carried out by adding saccharifying enzyme into 120-160U/g starch, and saccharifying at 45-60 deg.C for 20-60 min.
In one embodiment of the invention, the starchy raw material is one or more of cassava, corn, wheat and the like.
In one embodiment of the present invention, the amount of acetic acid added is 200 to 700mg/L of the saccharified solution.
The invention also provides the application of the method in preparing the alcohol-containing food or beverage.
Has the advantages that: according to the invention, the conversion rate of starch is improved to more than 89.6-90% by adding acetic acid. Compared with the prior art, the method has the greatest advantages that the starch conversion rate in the alcohol fermentation process can be improved, the generation of the byproduct glycerol can be reduced (about 20 percent can be reduced), and the added acetic acid cost is far lower than the profit generated by producing more alcohol (1 kg of acetic acid can improve the alcohol yield by about 17kg, the price of acetic acid is about 3 yuan/kg, and the price of alcohol is about 4.6 yuan/kg, so the cost of adding 1kg of acetic acid is 3 yuan, and the value of improving the alcohol yield is about 78 yuan). Because the current alcohol production technology is mature, the alcohol production scale can reach thousands of tons, therefore, even if the conversion rate is improved by 1-2%, the increased profit is considerable for alcohol production enterprises, the process of the invention is simple to operate, the yield stability among batches is good, and the invention has important promoting significance for the technical scheme of the alcohol production industry.
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FIG. 1 is a process flow diagram of the present invention.
Detailed Description
The invention will be further described with reference to examples in the drawings to which:
in the specific embodiment, the seed liquid takes saccharomyces cerevisiae as a fermentation strain, and the strain number is cultured to 2 multiplied by 106CFU/mL was considered as mature seed fluid.
Example 1
The invention relates to a method for improving the conversion rate of alcohol fermentation starch by adding acetic acid, which adopts the following process steps:
mixing the cassava powder with stirring water at a ratio of 1:2.5, adding high temperature resistant alpha-amylase (15U/g starch), stirring, heating to 95 deg.C, and maintaining for 120 min; cooling to 60 deg.C, adding diastase (120U/g starch), stirring, and saccharifying for 30 min. Cooling to 30 ℃ after saccharification is finished, using 15% saccharified liquid for preparing yeast seed liquid, adding acetic acid with the final concentration of 200mg/L into the residual saccharified liquid, adding mature seed liquid prepared by the 15% saccharified liquid, and starting fermentation. After fermentation, alcohol is obtained through processes of distillation, rectification and the like. As a blank, no acetic acid was added. The starch conversion of the experimental group was 89.6% higher than 88.1% of the blank.
Example 2
The invention relates to a method for improving the conversion rate of alcohol fermentation starch by adding acetic acid, which adopts the following process steps:
mixing corn flour and mixing water at a ratio of 1:3, adding high temperature resistant alpha-amylase (18U/g starch), stirring, heating to 95 deg.C, and maintaining for 120 min; cooling to 60 deg.C, adding saccharifying enzyme (150U/g starch), stirring, and saccharifying for 30 min. Cooling to 30 ℃ after saccharification is finished, using 10% of saccharification liquid to prepare yeast seed liquid, adding acetic acid with the final concentration of 300mg/L into the residual saccharification liquid, adding mature seed liquid prepared from the 15% of saccharification liquid, and starting fermentation. After fermentation, alcohol is obtained through processes of distillation, rectification and the like. As a blank, no acetic acid was added. The starch conversion of the experimental group was 90.8% higher than 88.9% of the blank.
Example 3
The invention relates to a method for improving the conversion rate of alcohol fermentation starch by adding acetic acid, which adopts the following process steps:
mixing cassava flour, wheat flour and corn flour with a material mixing water at a material-water ratio of 1:3, adding high temperature resistant alpha-amylase (16U/g starch), stirring well, heating to 95 deg.C and maintaining for 100 min; cooling to 60 deg.C, adding saccharifying enzyme (150U/g starch), stirring, and saccharifying for 30 min. Cooling to 30 ℃ after saccharification is finished, using 20% saccharified liquid for preparing yeast seed liquid, adding acetic acid with the final concentration of 500mg/L into the residual saccharified liquid, adding mature seed liquid prepared by the 15% saccharified liquid, and starting fermentation. After fermentation, alcohol is obtained through processes of distillation, rectification and the like. As a blank, no acetic acid was added. The starch conversion of the experimental group was 90.2% higher than 88.3% of the blank.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (4)
1. The method for alcohol fermentation is characterized by comprising the following process steps:
(1) mixing the crushed starchy raw materials with stirring water, adding liquefying enzyme, and heating for liquefaction;
(2) cooling after liquefaction, and adding saccharifying enzyme for saccharification;
(3) cooling after saccharification is finished, wherein the cooled saccharification liquid is divided into two parts, one part is directly used for preparing seed liquid, and the other part is added with acetic acid; inoculating the seed solution into the saccharification solution added with acetic acid, and starting alcoholic fermentation;
(4) after fermentation is finished, distilling to obtain alcohol;
wherein: adding 200-700 mg/L of acetic acid into the saccharification liquid; the starchy raw material is one or more mixed raw materials of cassava, corn and wheat; mixing the starchy raw material with mixing water in a material-water ratio of 1: 2-5; the liquefaction is carried out by adding high-temperature resistant alpha-amylase into 15-20U/g of starch, and reacting for 90-140 min at 90-95 ℃; the saccharification is carried out by adding saccharifying enzyme into 120-160U/g starch, and saccharifying for 20-60 min at 45-60 ℃.
2. A method for improving starch conversion rate in alcohol fermentation is characterized in that acetic acid is added into a fermentation system containing starchy materials in the alcohol fermentation process; adding 200-700 mg/L of acetic acid into the saccharification liquid;
the starchy raw material is one or more of cassava, corn and wheat.
3. The method according to claim 2, wherein acetic acid is added to the saccharified solution after saccharification to a final concentration of 300 to 900mg/L, and the seed solution is inoculated for fermentation.
4. Use of the method of any one of claims 1 to 3 for the preparation of an alcoholic food or beverage product.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109182393B (en) * | 2018-09-10 | 2021-03-02 | 江南大学 | Method for producing ethanol by recycling anaerobic digestion effluent and fine tower residual liquid |
| CN109182394B (en) * | 2018-09-10 | 2021-03-02 | 江南大学 | Method for producing ethanol by using grains as raw materials |
| CN109536539A (en) * | 2018-11-20 | 2019-03-29 | 江苏金茂源生物化工有限责任公司 | Ethyl alcohol preparation method |
Citations (4)
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| CN101633896A (en) * | 2009-08-27 | 2010-01-27 | 中国科学院微生物研究所 | Saccharmyces cerevisiae strain for resisting high-concentration acetic acid and application thereof |
| CN101880692A (en) * | 2010-07-06 | 2010-11-10 | 江南大学 | Alcohol fermentation method capable of improving raw material utilization rate |
| CN105368878A (en) * | 2014-08-12 | 2016-03-02 | 江苏花厅生物科技有限公司 | Method for producing alcohol by using manihot esculenta crantz as raw material |
| CN107299118A (en) * | 2016-04-15 | 2017-10-27 | 苏州昆蓝生物科技有限公司 | A kind of method of cassava anacidity fermenting and producing alcohol |
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2017
- 2017-12-18 CN CN201711367979.6A patent/CN107828827B/en active Active
Patent Citations (4)
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| CN101633896A (en) * | 2009-08-27 | 2010-01-27 | 中国科学院微生物研究所 | Saccharmyces cerevisiae strain for resisting high-concentration acetic acid and application thereof |
| CN101880692A (en) * | 2010-07-06 | 2010-11-10 | 江南大学 | Alcohol fermentation method capable of improving raw material utilization rate |
| CN105368878A (en) * | 2014-08-12 | 2016-03-02 | 江苏花厅生物科技有限公司 | Method for producing alcohol by using manihot esculenta crantz as raw material |
| CN107299118A (en) * | 2016-04-15 | 2017-10-27 | 苏州昆蓝生物科技有限公司 | A kind of method of cassava anacidity fermenting and producing alcohol |
Non-Patent Citations (3)
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