CN108949585A - A kind of cultural method of the agricultural fungi microbe of semi-open bed - Google Patents
A kind of cultural method of the agricultural fungi microbe of semi-open bed Download PDFInfo
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- CN108949585A CN108949585A CN201810865977.8A CN201810865977A CN108949585A CN 108949585 A CN108949585 A CN 108949585A CN 201810865977 A CN201810865977 A CN 201810865977A CN 108949585 A CN108949585 A CN 108949585A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention relates to the methods of agricultural fungi microbe culture, especially a kind of cultural method of the agricultural fungi microbe of semi-open bed, specific steps include: medium treatment: culture medium is added water to aqueous 55--65%, spontaneous fermentation processing, when temperature rises to 60 °C or more, kept for 3 days；It makes the bed: the culture medium fermented is laid on culture apparatus；Inoculation: being inoculated with by the 3-5% of culture medium weight, covers layer of plastic film above it after the completion of seed inoculation；Fermented and cultured: air after filtering is filled with culture medium, and is kept for 28-35 °C of temperature, cultivates 5--8 days；Water content 55--65% is kept during culture, if moisture is lower than 55% during culture, can moisturizing to 60%；Finished product production: after the completion of culture, constantly stirring culture medium, increase ventilatory capacity, promotes the quick dehydration of culture medium to 15%-25%, after culture medium is mixed, detects viable bacteria content, be finished product after up to standard, be packed and stored.
Description
Technical field
The present invention relates to the method for agricultural fungi microbe culture, especially a kind of agricultural fungi microbe of semi-open bed
Cultural method.
Background technique
Rotary spherical digester is a kind of rotary batch cooking pulping equipment.This is as spherical shape, mainly by sphere, base, transmission device
It is formed Deng part, sphere is welded or riveted by steel plate and is made；It is provided with charging hole in sphere vertical centerline position, for charging and sending
Enter cooking liquor use, the steam and discharge condensed water of boiling heating are passed through from tubular journal.At present in agricultural fungi microbe
The mode that rotary spherical digester culture is often used during culture, carries out general when agricultural fungi microbe culture in this way
The fermentation time needed is longer, and usually at 5-7 days or so, and the equipment used during entirely cultivating is varied, throws
Enter that cost is larger, energy consumption is high but production capacity be limited serious, and use conventional methods cultivated during to culture
Environmental requirement is relatively stringent, scraps once pollution occur and will lead to entire incubation, therefore existing this utilize is steamed
The mode that the equipment such as ball carry out agricultural fungi microbe culture cannot meet the needs of market well.
Summary of the invention
The present invention is at least to solve technical solution used by one of above-mentioned technical problem to be: a kind of semi-open bed is agricultural
The cultural method of fungi microbe, specific steps include:
S1: medium treatment: culture medium is added water into water content 55--65%, spontaneous fermentation handles culture medium, and temperature rises to 60 °C
When above, kept for 3 days, with this condition, the overwhelming majority can be dead for the miscellaneous bacteria in culture medium, only remain can fiber bacterium resistant to high temperature,
Hemicellulose bacterium, actinomyces etc..
When by culture medium make thinner tiling to it is culture bed upper when, culture medium temperature will drop to 28--35 °C, at this point, in culture medium
Can fiber bacterium resistant to high temperature, hemicellulose bacterium, actinomyces etc. stopped growing, can 28--35 °C of normal growth miscellaneous bacteria preceding
It has been killed in the hot fermentation in one stage.It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, is there is agricultural miscellaneous bacteria flora
In the case where advantage, agricultural fungi occupies rapidly culture medium, quickly breeds.
The purpose and effect for keeping moisture content in medium 55--65% are: agricultural fungi microbe is raw under this moisture condition
Length is most fast.Culture base location is seized rapidly, quickly breeds, reaches the competitive growth advantage with miscellaneous bacteria.
After hot fermentation is handled, part macro-nutrients are decomposed small molecule nutriment culture medium, can be by
Agricultural fungi is quickly absorbed and utilized.Simultaneously within the scope of 28--35 °C, with the miscellaneous bacteria of agricultural fungi microbe competitive growth by
Hot fermentation early period kills.
It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, it is agricultural true in the case where there is agricultural miscellaneous bacteria flora advantage
Bacterium occupies rapidly culture medium, quickly breeds.
S2: it makes the bed: the culture medium fermented is laid on culture apparatus；
S3: inoculation: being inoculated with by the 3-5% of culture medium weight, and it is thin to cover one layer of aseptic plastic after the completion of seed inoculation above it
Film；
S4: inoculation: air after filtering is filled with culture medium, and is kept for 28-35 °C of temperature, cultivates 5--8 days；It is protected during culture
Hold water content 55--65%, if moisture is lower than 55% during culture, can moisturizing to 60%；
The purpose of 28-35 °C of culture medium temperature of maintenance, water content 55--65% is the agricultural micro- life of fungi under this temperature, moisture condition
Object growth is most fast, can achieve the purpose that fast-growth, inhibit miscellaneous bacteria.
S5: finished product production: after the completion of culture, culture medium is constantly stirred, ventilatory capacity is increased, promotes the quick dehydration of culture medium extremely
15%-25% after mixing culture medium, detects viable bacteria content, is finished product after up to standard, is packed and stored.
Preferably, the longitudinal section of culture medium described in S1 is trapezoidal, and the height of the ladder is 1.5m, base width is
1.5m；The volume of the culture medium should be greater than 3m3；Depending on every batch of culture volume is with culture bed area, it is with 30--50m3
Preferably.
Preferably, the laying depth of culture medium is 30-50cm in the S2.
Preferably, the culture apparatus includes a culture bed frame body, and interval fixation from top to bottom is set on culture bed frame body
The laying platform for having several horizontal, the laying platform are put down for holding the culture medium fermented described in S1 in each be laid with
It is respectively equipped with a heating element on the top surface of platform, temperature control is carried out to the culture medium being laid with thereon by heating element, is convenient for
The temperature with regulation culture base is grasped in real time, and a gas supply dress for ventilating to culture medium is respectively equipped on culture bed frame body
It sets, feeder is mainly used to be passed through into step described in S5 by the processed air of air filter module.
The culture of agricultural fungi microbe needs to consume a large amount of oxygen, by being passed through compressed air, constantly to culture
Bed supplemental oxygen, meets the needs of agricultural fungi microbe growth and breeding.The purpose for being passed through filtered air is to filter out air
In miscellaneous bacteria because air contains the various miscellaneous bacterias of numerous quantity, after active carbon vent material, miscellaneous bacteria in air
Achieve the purpose that purification, prevents the miscellaneous bacteria in air that culture medium is added.
The purpose and benefit that two layers culture medium interval is laid be easy for temperature control, moisture regulation, ventilation it is unimpeded, convenient for seeing
Examine monitoring.
Preferably, the adjacent spacing distance being laid between platform is 15-20cm.
Preferably, the heating element includes that the electric heating cloth being laid on platform top surface is arranged in, and the electric heating cloth is logical
It crosses conducting wire to be connected with external power supply, is equipped with a temperature sensor for being used to detect culture medium temperature being laid with above platform, it is described
Temperature sensor is connected by conducting wire with the signal receiving end of external display, between each electric heating cloth and external power supply
It is respectively equipped with a regulating switch on conducting wire, the temperature in culture medium and real-time display are obtained in display by temperature sensor
On, the model of display and temperature sensor also only needs to pass through using general model on the market at present in connection
Signal wire is directly connected to, those skilled in the art can skilled operation, when the temperature for observing culture medium is too low or mistake
The heating efficiency of regulation electric heating cloth can be carried out when high by regulating switch, and then realizes the regulation to temperature, certainly
It can be using automatic temperature-controlled monitoring and setting lowest detection temperature value, when being heated when temperature is lower than minimum, being higher than peak
Stop heating and keeping the temperature.
Preferably, the feeder includes an air blower, and an air mistake is equipped on the supervisor in the exit of air blower
Filter component, several blast pipes be connected with by multiple-pass joint on supervisor, the air outlet of each blast pipe respectively with the training
It supports and is connected inside base, by the way that multiple blast pipes are arranged in each top with the laying platform of culture medium cooperation, gas can be made
Body is more fully passed into culture medium.
Preferably, the air filter module includes a filtering spherical shell being arranged on supervisor, is equipped in filtering spherical shell
Active carbon can effectively filter the general air in culture medium, and making the air entered in culture medium is sterile shape
State improves the fermenting speed of agricultural fungi microbe, achievees the purpose that fast culture.
Beneficial effect possessed by the present invention is,.
It is had the advantage that under present condition using this technology
1. investment reduction: producing agricultural fungi microbe by the production method of microflora microbial inoculum, need a large amount of equipment, factory
Room investment, the construction period is long, and investment in fixed assets is big.Equipment investment just corresponds to 1st/20th of conventional method, construction week
Phase is only 1/10th.
2. energy-saving and environmental protection: the production of conventional agricultural fungi microbe needs mating large-sized boiler, and energy consumption is big, industry
" three wastes " discharge amount is big, and general small business is difficult to bear the policy requirements of environment protection treating, safety in production.It is simply given birth to using this law
Produce agricultural fungi, the construction period is short, is not restricted by environment, the energy, equipment, simple and easy.
3. easy to operate: direct labor need to only can be operated by basic training.Equipment is simple, operation is easy, safely may be used
It leans on.
4. high-efficient: producing agricultural fungi using this method, fast and convenient, volume production is fast, and yield is big.
Detailed description of the invention
Fig. 1 is the culture apparatus structural schematic diagram in the present invention.
In figure, 1, culture bed frame body；2, it is laid with platform；3, electric heating cloth；4, temperature sensor；5, display；6, it adjusts
Section switch；7, air blower；8, blast pipe；9, spherical shell is filtered；10, active carbon；11, it is responsible for；12, multiple-pass joint.
Specific embodiment
In order to clarify the technical characteristics of the invention, below by specific embodiment, and its attached drawing is combined, to this hair
It is bright to be described in detail.
Embodiment 1:
A kind of cultural method of the agricultural fungi microbe of semi-open bed, specific steps include:
S1: medium treatment: culture medium is added water into water content 55%, spontaneous fermentation handles culture medium, and temperature rises to 60 °C or more
When, holding 3 days, with this condition, the overwhelming majority can be dead for the miscellaneous bacteria in culture medium, only remains energy fiber bacterium resistant to high temperature, half fiber
Tie up bacterium, actinomyces etc..
When by culture medium make thinner tiling to it is culture bed upper when, culture medium temperature will drop to 28 °C, at this point, culture medium in be resistant to
The fiber bacterium of high temperature, hemicellulose bacterium, actinomyces etc. have stopped growing, can 28 °C of normal growths miscellaneous bacteria in previous stage
It has been killed in hot fermentation.It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, in the feelings for having agricultural miscellaneous bacteria flora advantage
Under condition, agricultural fungi occupies rapidly culture medium, quickly breeds.
It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, it is agricultural true in the case where there is agricultural miscellaneous bacteria flora advantage
Bacterium occupies rapidly culture medium, quickly breeds.
S2: it makes the bed: the culture medium fermented is laid on culture apparatus；
S3: by 3% inoculation of culture medium weight, one layer of sterile plastic sheet inoculation: is covered above it after the completion of seed inoculation；
S4: inoculation: air after filtering is filled with culture medium, and is kept for 28-35 °C of temperature, cultivates 5--8 days；It is protected during culture
Hold water content 55--65%, if moisture is lower than 55% during culture, can moisturizing to 60%；
The purpose of 28-35 °C of culture medium temperature of maintenance, water content 55--65% is the agricultural micro- life of fungi under this temperature, moisture condition
Object growth is most fast, can achieve the purpose that fast-growth, inhibit miscellaneous bacteria.
S5: finished product production: after the completion of culture, culture medium is constantly stirred, ventilatory capacity is increased, promotes the quick dehydration of culture medium extremely
15%-25% after mixing culture medium, detects viable bacteria content, is finished product after up to standard, is packed and stored.
Preferably, the longitudinal section of culture medium described in S1 is trapezoidal, and the height of the ladder is 1.5m, base width is
1.5m；The volume of the culture medium should be greater than 3m3；Depending on every batch of culture volume is with culture bed area, it is advisable with 30m3.
Preferably, the laying depth of culture medium is 30cm in the S2.
Preferably, the culture apparatus includes a culture bed frame body, and interval fixation from top to bottom is set on culture bed frame body
The laying platform for having several horizontal, the laying platform are put down for holding the culture medium fermented described in S1 in each be laid with
It is respectively equipped with a heating element on the top surface of platform, temperature control is carried out to the culture medium being laid with thereon by heating element, is convenient for
The temperature with regulation culture base is grasped in real time, and a gas supply dress for ventilating to culture medium is respectively equipped on culture bed frame body
It sets, feeder is mainly used to be passed through into step described in S5 by the processed air of air filter module.
The culture of agricultural fungi microbe needs to consume a large amount of oxygen, by being passed through compressed air, constantly to culture
Bed supplemental oxygen, meets the needs of agricultural fungi microbe growth and breeding.The purpose for being passed through filtered air is to filter out air
In miscellaneous bacteria because air contains the various miscellaneous bacterias of numerous quantity, after active carbon vent material, miscellaneous bacteria in air
Achieve the purpose that purification, prevents the miscellaneous bacteria in air that culture medium is added.
The purpose and benefit that two layers culture medium interval is laid be easy for temperature control, moisture regulation, ventilation it is unimpeded, convenient for seeing
Examine monitoring.
Preferably, the adjacent spacing distance being laid between platform is 15cm.
Preferably, the heating element includes that the electric heating cloth being laid on platform top surface is arranged in, and the electric heating cloth is logical
It crosses conducting wire to be connected with external power supply, is equipped with a temperature sensor for being used to detect culture medium temperature being laid with above platform, it is described
Temperature sensor is connected by conducting wire with the signal receiving end of external display, between each electric heating cloth and external power supply
It is respectively equipped with a regulating switch on conducting wire, the temperature in culture medium and real-time display are obtained in display by temperature sensor
On, the model of display and temperature sensor also only needs to pass through using general model on the market at present in connection
Signal wire is directly connected to, those skilled in the art can skilled operation, when the temperature for observing culture medium is too low or mistake
The heating efficiency of regulation electric heating cloth can be carried out when high by regulating switch, and then realizes the regulation to temperature, certainly
It can be using automatic temperature-controlled monitoring and setting lowest detection temperature value, when being heated when temperature is lower than minimum, being higher than peak
Stop heating and keeping the temperature.
Preferably, the feeder includes an air blower, and an air mistake is equipped on the supervisor in the exit of air blower
Filter component, several blast pipes be connected with by multiple-pass joint on supervisor, the air outlet of each blast pipe respectively with the training
It supports and is connected inside base, by the way that multiple blast pipes are arranged in each top with the laying platform of culture medium cooperation, gas can be made
Body is more fully passed into culture medium.
Preferably, the air filter module includes a filtering spherical shell being arranged on supervisor, is equipped in filtering spherical shell
Active carbon can effectively filter the general air in culture medium, and making the air entered in culture medium is sterile shape
State improves the fermenting speed of agricultural fungi microbe, achievees the purpose that fast culture.
Embodiment 2:
A kind of cultural method of the agricultural fungi microbe of semi-open bed, specific steps include:
S1: medium treatment: culture medium is added water into water content 60%, spontaneous fermentation handles culture medium, and temperature rises to 60 °C or more
When, holding 3 days, with this condition, the overwhelming majority can be dead for the miscellaneous bacteria in culture medium, only remains energy fiber bacterium resistant to high temperature, half fiber
Tie up bacterium, actinomyces etc..
When by culture medium make thinner tiling to it is culture bed upper when, culture medium temperature will drop to 35 °C, at this point, culture medium in be resistant to
The fiber bacterium of high temperature, hemicellulose bacterium, actinomyces etc. have stopped growing, can 35 °C of normal growths miscellaneous bacteria in previous stage
It has been killed in hot fermentation.It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, in the feelings for having agricultural miscellaneous bacteria flora advantage
Under condition, agricultural fungi occupies rapidly culture medium, quickly breeds.
It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, it is agricultural true in the case where there is agricultural miscellaneous bacteria flora advantage
Bacterium occupies rapidly culture medium, quickly breeds.
S2: it makes the bed: the culture medium fermented is laid on culture apparatus；
S3: by 5% inoculation of culture medium weight, one layer of sterile plastic sheet inoculation: is covered above it after the completion of seed inoculation；
S4: inoculation: air after filtering is filled with culture medium, and is kept for 28-35 °C of temperature, cultivates 5--8 days；It is protected during culture
Hold water content 55--65%, if moisture is lower than 55% during culture, can moisturizing to 60%；
The purpose of 28-35 °C of culture medium temperature of maintenance, water content 55--65% is the agricultural micro- life of fungi under this temperature, moisture condition
Object growth is most fast, can achieve the purpose that fast-growth, inhibit miscellaneous bacteria.
S5: finished product production: after the completion of culture, culture medium is constantly stirred, ventilatory capacity is increased, promotes the quick dehydration of culture medium extremely
18%, after culture medium is mixed, viable bacteria content is detected, is finished product after up to standard, is packed and stored.
Preferably, the longitudinal section of culture medium described in S1 is trapezoidal, and the height of the ladder is 1.5m, base width is
1.5m；The volume of the culture medium should be greater than 3m3；Depending on every batch of culture volume is with culture bed area, it is advisable with 30m3.
Preferably, the laying depth of culture medium is 30cm in the S2.
Preferably, the culture apparatus includes a culture bed frame body, and interval fixation from top to bottom is set on culture bed frame body
The laying platform for having several horizontal, the laying platform are put down for holding the culture medium fermented described in S1 in each be laid with
It is respectively equipped with a heating element on the top surface of platform, temperature control is carried out to the culture medium being laid with thereon by heating element, is convenient for
The temperature with regulation culture base is grasped in real time, and a gas supply dress for ventilating to culture medium is respectively equipped on culture bed frame body
It sets, feeder is mainly used to be passed through into step described in S5 by the processed air of air filter module.
The culture of agricultural fungi microbe needs to consume a large amount of oxygen, by being passed through compressed air, constantly to culture
Bed supplemental oxygen, meets the needs of agricultural fungi microbe growth and breeding.The purpose for being passed through filtered air is to filter out air
In miscellaneous bacteria because air contains the various miscellaneous bacterias of numerous quantity, after active carbon vent material, miscellaneous bacteria in air
Achieve the purpose that purification, prevents the miscellaneous bacteria in air that culture medium is added.
The purpose and benefit that two layers culture medium interval is laid be easy for temperature control, moisture regulation, ventilation it is unimpeded, convenient for seeing
Examine monitoring.
Preferably, the adjacent spacing distance being laid between platform is 20cm.
Preferably, the heating element is identical as 1 structure of embodiment.
Preferably, the air filter module is identical as 1 structure of embodiment.
Embodiment 3:
A kind of cultural method of the agricultural fungi microbe of semi-open bed, specific steps include:
S1: medium treatment: culture medium is added water into water content 65%, spontaneous fermentation handles culture medium, and temperature rises to 60 °C or more
When, holding 3 days, with this condition, the overwhelming majority can be dead for the miscellaneous bacteria in culture medium, only remains energy fiber bacterium resistant to high temperature, half fiber
Tie up bacterium, actinomyces etc..
When by culture medium make thinner tiling to it is culture bed upper when, culture medium temperature will drop to 30 °C, at this point, culture medium in be resistant to
The fiber bacterium of high temperature, hemicellulose bacterium, actinomyces etc. have stopped growing, can 30 °C of normal growths miscellaneous bacteria in previous stage
It has been killed in hot fermentation.It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, in the feelings for having agricultural miscellaneous bacteria flora advantage
Under condition, agricultural fungi occupies rapidly culture medium, quickly breeds.
It is inoculated with agriculture fungi strain at this time, is just being suitable for its growth, it is agricultural true in the case where there is agricultural miscellaneous bacteria flora advantage
Bacterium occupies rapidly culture medium, quickly breeds.
S2: it makes the bed: the culture medium fermented is laid on culture apparatus；
S3: by 5% inoculation of culture medium weight, one layer of sterile plastic sheet inoculation: is covered above it after the completion of seed inoculation；
S4: inoculation: air after filtering is filled with culture medium, and is kept for 28-35 °C of temperature, cultivates 5--8 days；It is protected during culture
Hold water content 55--65%, if moisture is lower than 55% during culture, can moisturizing to 60%；
The purpose of 28-35 °C of culture medium temperature of maintenance, water content 55--65% is the agricultural micro- life of fungi under this temperature, moisture condition
Object growth is most fast, can achieve the purpose that fast-growth, inhibit miscellaneous bacteria.
S5: finished product production: after the completion of culture, culture medium is constantly stirred, ventilatory capacity is increased, promotes the quick dehydration of culture medium extremely
18%, after culture medium is mixed, viable bacteria content is detected, is finished product after up to standard, is packed and stored.
Preferably, the longitudinal section of culture medium described in S1 is trapezoidal, and the height of the ladder is 1.5m, base width is
1.5m；The volume of the culture medium should be greater than 3m3；Depending on every batch of culture volume is with culture bed area, it is advisable with 30m3.
Preferably, the laying depth of culture medium is 25cm in the S2.
Preferably, the culture apparatus includes a culture bed frame body, and interval fixation from top to bottom is set on culture bed frame body
The laying platform for having several horizontal, the laying platform are put down for holding the culture medium fermented described in S1 in each be laid with
It is respectively equipped with a heating element on the top surface of platform, temperature control is carried out to the culture medium being laid with thereon by heating element, is convenient for
The temperature with regulation culture base is grasped in real time, and a gas supply dress for ventilating to culture medium is respectively equipped on culture bed frame body
It sets, feeder is mainly used to be passed through into step described in S5 by the processed air of air filter module.
The culture of agricultural fungi microbe needs to consume a large amount of oxygen, by being passed through compressed air, constantly to culture
Bed supplemental oxygen, meets the needs of agricultural fungi microbe growth and breeding.The purpose for being passed through filtered air is to filter out air
In miscellaneous bacteria because air contains the various miscellaneous bacterias of numerous quantity, after active carbon vent material, miscellaneous bacteria in air
Achieve the purpose that purification, prevents the miscellaneous bacteria in air that culture medium is added.
The purpose and benefit that two layers culture medium interval is laid be easy for temperature control, moisture regulation, ventilation it is unimpeded, convenient for seeing
Examine monitoring.
Preferably, the adjacent spacing distance being laid between platform is 20cm.
Preferably, the heating element is identical as 1 structure of embodiment.
Preferably, the air filter module is identical as 1 structure of embodiment.
Test example:
Using this law, Shandong Fei Ye Co., Ltd has carried out the trial production of multiple batches of agricultural fungi microbe fertilizer, and effect is bright
Aobvious, specific performance parameter is as follows:
Above-mentioned specific embodiment cannot function as limiting the scope of the invention, and those skilled in the art are come
It says, any alternate modification or transformation made to embodiment of the present invention are fallen within the scope of protection of the present invention.
Place is not described in detail by the present invention, is the well-known technique of those skilled in the art of the present technique.
Claims (8)
1. a kind of cultural method of the agricultural fungi microbe of semi-open bed, it is characterised in that: specific steps include: S1: culture
Base processing: culture medium is added water into water content 55--65%, spontaneous fermentation processing is kept for 3 days when temperature rises to 60 °C or more；
S2: it makes the bed: the culture medium fermented is laid on culture apparatus；
S3: inoculation: being inoculated with by the 3-5% of culture medium weight, covers layer of plastic film above it after the completion of seed inoculation；
S4: inoculation: air after filtering is filled with culture medium, and is kept for 28-35 °C of temperature, cultivates 5--8 days；It is protected during culture
Hold water content 55--65%；
S5: finished product production: after the completion of culture, culture medium is constantly stirred, ventilatory capacity is increased, promotes the quick dehydration of culture medium to 15%-
25%, after culture medium is mixed, viable bacteria content is detected, is finished product after up to standard, is packed and stored.
2. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 1, it is characterised in that: S1
Described in the longitudinal section of culture medium be triangle, the height of the triangle is 1.5m, base width 1.5m.
3. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 1, it is characterised in that: institute
The laying depth for stating culture medium in S2 is 30-50cm.
4. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 3, it is characterised in that: institute
Stating culture apparatus includes a culture bed frame body, and interval is fixed with several horizontal layings from top to bottom on culture bed frame body
Platform, the laying platform are respectively equipped on each top surface for being laid with platform for holding the culture medium fermented described in S1
One heating element is respectively equipped with a feeder for ventilating to culture medium on culture bed frame body.
5. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 4, it is characterised in that: institute
Stating the adjacent spacing distance being laid between platform is 15-20cm.
6. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 5, it is characterised in that: institute
Stating heating element includes that the electric heating cloth being laid on platform top surface is arranged in, and the electric heating cloth passes through conducting wire and external power supply phase
Even, it is being laid with above platform equipped with one for detecting the temperature sensor of culture medium temperature, the temperature sensor passes through conducting wire
It is connected with the signal receiving end of external display, is respectively equipped with a tune on the conducting wire between each electric heating cloth and external power supply
Section switch.
7. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 6, it is characterised in that: institute
Stating feeder includes an air blower, and an air filter module is equipped on the supervisor in the exit of air blower, is led on supervisor
It crosses multiple-pass joint and is connected with several blast pipes, the air outlet of each blast pipe is connected with inside the culture medium respectively.
8. a kind of cultural method of semi-open agricultural fungi microbe of bed according to claim 7, it is characterised in that: institute
Stating air filter module includes a filtering spherical shell being arranged on supervisor, is equipped with active carbon in filtering spherical shell.
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| CN107674840A (en) * | 2017-11-20 | 2018-02-09 | 中国农业大学 | A kind of method of solid state fermentation production disinsection fungal-aspergillus oryzae spore |
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