CN108936700A - A method of extracting dietary fiber from hickory chick - Google Patents
A method of extracting dietary fiber from hickory chick Download PDFInfo
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- CN108936700A CN108936700A CN201810626503.8A CN201810626503A CN108936700A CN 108936700 A CN108936700 A CN 108936700A CN 201810626503 A CN201810626503 A CN 201810626503A CN 108936700 A CN108936700 A CN 108936700A
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- 238000000034 method Methods 0.000 title claims abstract description 68
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- 230000004151 fermentation Effects 0.000 claims abstract description 27
- 239000000835 fiber Substances 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000000284 extract Substances 0.000 claims abstract description 11
- 239000000706 filtrate Substances 0.000 claims description 62
- 239000007788 liquid Substances 0.000 claims description 37
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- 239000007787 solid Substances 0.000 claims description 20
- 238000001914 filtration Methods 0.000 claims description 18
- 235000019441 ethanol Nutrition 0.000 claims description 16
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- 235000020188 drinking water Nutrition 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 241000228245 Aspergillus niger Species 0.000 claims description 10
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 10
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims description 10
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 8
- 239000012043 crude product Substances 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
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- 238000002360 preparation method Methods 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 4
- 239000006137 Luria-Bertani broth Substances 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
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- 230000001766 physiological effect Effects 0.000 abstract description 8
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The method that the invention discloses a kind of to extract dietary fiber from hickory chick, is related to health food processing technique field.Hickory chick is boiled and is fermented except carrying out access microorganism after miscellaneous bacteria, successively extracts insoluble diedairy fiber and soluble dietary fiber by the of the invention method that dietary fiber is extracted from hickory chick.The dietary fiber in hickory chick is extracted using the method for microbial fermentation, obtained dietary fiber is in good taste, physiological activity is high；The insoluble diedairy fiber and soluble dietary fiber in hickory chick are successively extracted, extraction efficiency is high；After extracting insoluble diedairy fiber, the nutriments such as amino acid and soluble dietary fiber are successively settled out by the way of ethanol precipitation, on the one hand the purity that soluble dietary fiber can be improved, on the other hand can sufficiently extract the different nutriments in hickory chick, improve the utilization rate of hickory chick.
Description
Technical field
The present invention relates to health food processing technique field, especially a kind of side that dietary fiber is extracted from hickory chick
Method.
Background technique
Dietary fiber is a kind of complicated high-molecular compound, can be divided into water-soluble dietary fiber according to deliquescent difference
With water insoluble dietary fiber two major classes.Water insoluble dietary fiber refer in dietary fiber not by alimentary canal enzymic digestion and not
Hot water soluble part non-starch class formation polysaccharide, it is mainly the constituent of cell wall, including cellulose, hemicellulose
Element, lignin, chitosan and vegetable wax etc..Water-soluble dietary fiber refers in dietary fiber not to be digested by body digestive enzyme,
But a kind of non-starch polysaccharide for dissolving in hot water, and being separated by the ethanol precipitation of certain volume mainly includes in plant cell
Water-soluble storage material and secretion, additionally include part microbial polysaccharide and synthesis polysaccharide, and composition is mainly some glue
Substance.Modern medicine circle, scientific circles' research have shown that, though dietary fiber is not absorbed in human body, help to adjust internal
The metabolism of carbohydrate and lipid and the absorption of minerals, can significantly reduce blood lipid and internal levels of peroxide, to obesity,
Hypertension, coronary atherosclerosis, gall stone, diabetes, colonopathy, hyperlipidemia, heart disease and cardiovascular disease etc. have one
Fixed preventive and therapeutic action also prevents diarrhea, protection liver and improves the physiological functions such as immunity.
The method for extracting dietary fiber both at home and abroad at present mainly has hot water extraction method, chemical leaching test, enzyme process etc..Hot water mentions
Simple process is followed the example of, but recovery rate is not high.Chemical leaching test preparation cost is lower, but there are drawbacks in environmental protection.Enzyme process is
The other compositions in degradation raw material are gone with various enzymes, this method is efficient, pollution-free, and specificity is strong, but at high cost.
Hickory chick is also known as sheep tripe dish, delicious hickory chick, sheep mushroom, is a kind of integration of drinking and medicinal herbs mushroom of preciousness.It is sought in hickory chick
Support material abundance, according to surveying and determination, hickory chick 20-30% containing crude protein, carbohydrate 35-40%, also contain there are many amino acid and
Dietary fiber abundant, and contained dietary fiber is very palatable, thus be considered as best one of dietary fiber sources.
If being extracted using traditional diet fiber extraction method extraction to the dietary fiber in hickory chick, extraction efficiency is low, and
The content of obtained soluble dietary fiber is low, moreover, cannot make full use of, make to other nutritions in hickory chick
It is wasted at hickory chick.
Summary of the invention
Goal of the invention of the invention is: in view of the above problems, providing one kind and extracts diet fibre from hickory chick
The method of dimension, this method extract dietary fiber using the method for microbial fermentation from hickory chick, can obtain soluble dietary fibre
Peacekeeping insoluble diedairy fiber, and other nutriments in isolated hickory chick, while soluble dietary fibre content height,
Physiological activity is high.
The technical solution adopted by the invention is as follows:
A method of extracting dietary fiber from hickory chick comprising following steps:
(1) take fresh hickory chick to clean, be 1:0.5-1.5(g:L according to solid-liquid ratio) drinking water is added, 20- is boiled in heating
40min；
(2) after being cooled to room temperature, hickory chick band water is transferred to fermentor together, and access zymocyte liquid sealing and fermenting；
(3) the first filtrate and the first filter residue is obtained by filtration；First filter residue is insoluble diedairy fiber crude product；
(4) 1/2-1/3 that the first filtrate to original volume is concentrated obtains concentration filtrate, and the ethyl alcohol of 2 times of concentration filtrate volumes is added, mixes
After even, 1-2h is stood, the second filtrate and the second filter residue is obtained by filtration.
(5) ethyl alcohol of 2 times of second filtrate volume is added into the second filtrate, after mixing, stands 1-2h, third is obtained by filtration
Filtrate and third filter residue；Third filter residue is soluble dietary fiber crude product.
By adopting the above-described technical solution, the method using microbial fermentation mentions the dietary fiber in hickory chick
It takes, obtained dietary fiber is in good taste, physiological activity is high；It boils and removes miscellaneous bacteria, guarantee the strain purity of fermentation process, guarantee to produce
Quality；Insoluble diedairy fiber and soluble dietary fiber therein are successively extracted, extraction efficiency is high；Due to soluble meals
Food fiber can be separated out by 4 times of volume ethanols, before this, first precipitated the nutriments such as amino acid with 2 times of volume ethanols and analysed
Out, i.e., amino acid etc. and soluble dietary fiber are successively precipitated using ethanol precipitation, on the one hand can improve soluble dietary
On the other hand the purity of fiber can sufficiently extract the different nutriments in hickory chick, improve the utilization rate of hickory chick.
A kind of method that dietary fiber is extracted from hickory chick of the invention, in step (1), the hickory chick after cleaning is broken
Granularity is broken to boil to heat after the particle of 0.8-1.5cm.
By adopting the above-described technical solution, broken hickory chick improves fermentation efficiency convenient for being utilized by thallus.
A kind of method for extracting dietary fiber from hickory chick of the invention, step (2) zymocyte liquid is by concentration ratio
It is formed for the streptococcus thermophilus, aspergillus niger, lactobacillus bulgaricus of 1:0.5-0.8:0.1-0.3, the concentration of streptococcus thermophilus is
(4-8) × 107CFU/mL。
By adopting the above-described technical solution, streptococcus thermophilus, aspergillus niger mainly will using divide in hickory chick crude protein and
Carbohydrate ferments, and is decomposed；Lactobacillus bulgaricus metabolic process produces acid to maintain fermentation liquid slant acidity, promotes
Insoluble diedairy fiber is converted into soluble dietary fiber, improves the content of soluble dietary fiber.
A kind of method for extracting dietary fiber from hickory chick of the invention, the additional amount of zymocyte liquid are hickory chick quality
0.5-5%.
A kind of method for extracting dietary fiber from hickory chick of the invention, hickory chick quality 1-3% is added into fermentor
Skimmed milk power, 0.3-0.5% glucose.
By adopting the above-described technical solution, skimmed milk power and glucose are nutrition needed for thallus afterfermentation, bacterium is improved
The fermentation activity of body improves the fermentation rate of hickory chick.
A kind of method for extracting dietary fiber from hickory chick of the invention, is sealed by fermentation and continues 24-48h, fermentation process
Temperature is 30-45 DEG C.
Fermentation time is appropriately extended and is conducive to lactic acid generation, insoluble diedairy fiber is promoted to be converted into soluble dietary fibre
Dimension, fermentation time is too long, and secondary metabolite and harmful substance increase, and influences thalli growth.
A kind of method for extracting dietary fiber from hickory chick of the invention, carries out each strain in the zymocyte liquid
Fermentor is accessed after domestication again；Domestication process include: respectively by each strain be seeded to solid LB media carry out activation 12-16 obtain
To activated seed, activated seed is accessed into culture 8-12h in LB liquid medium and obtains activation bacterium solution, then will activation bacterium solution inoculation
12-16h is cultivated into hickory chick solid medium to get the strain after domestication.
A kind of method for extracting dietary fiber from hickory chick of the invention, hickory chick solid medium is by common LB solid
Culture medium is 1:2 composition according to volume ratio with hickory chick extracting solution, and hickory chick extracting solution is prepared via a method which: will be fresh dry
Net hickory chick is 1:1(g:L according to solid-liquid ratio) drinking water is added, 30min is boiled in heating, is cooled to room temperature rear slurrying, crosses three layers
Take filtrate up to hickory chick extracting solution after gauze.
In above-mentioned technical proposal, tamed strain is substantially the process for selecting dominant bacteria.It is first sorted out own growth
The high strain of activity, then picks out in the high strain of growth activity again and is suitble to grow strain in hickory chick culture medium, with
This strain picked out, activity itself is high, high to the utilization rate of nutriment in hickory chick, is able to carry out high-speed fermentation.
A kind of method that dietary fiber is extracted from hickory chick of the invention in step (4), is first added into concentration filtrate
4% sodium carbonate, the pH for adjusting concentration filtrate is 6.8-7.4, then adds the ethyl alcohol of 2 times of volumes.
By adopting the above-described technical solution, concentration filtrate is adjusted to weakly acidic pH, the solubility of amino acid is reduced, favorably
In the Precipitation of amino acid, the impurity content in the soluble dietary fiber being settled out is reduced.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1. extracted using the method for microbial fermentation to the dietary fiber in hickory chick, obtained dietary fiber is in good taste,
Physiological activity is high.
2. successively extracting the insoluble diedairy fiber and soluble dietary fiber in hickory chick, extraction efficiency is high.
3. being successively settled out amino acid etc. and soluble dietary using ethanol precipitation after extracting insoluble diedairy fiber
On the one hand fiber can improve the purity of soluble dietary fiber, on the other hand can sufficiently extract the different nutrients in hickory chick
Matter improves the utilization rate of hickory chick.
Detailed description of the invention
Examples of the present invention will be described by way of reference to the accompanying drawings, in which:
Fig. 1 is the process flow chart of the method provided by the invention that dietary fiber is extracted from hickory chick.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated,
It is replaced by other equivalent or with similar purpose alternative features.That is, unless specifically stated, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The method that the present embodiment provides a kind of from hickory chick extracts dietary fiber, this method using microbial fermentation method from
Dietary fiber is extracted in hickory chick, can obtain soluble dietary fiber and insoluble diedairy fiber, and in isolated hickory chick
Other nutriments, while soluble dietary fibre content is high, physiological activity is high.This method includes the following steps:
Step 1: taking fresh hickory chick, clean up, and is that drinking water is added in 1:0.5 according to solid-liquid ratio, and 20min is boiled in heating,
After being cooled to room temperature, hickory chick and water are transferred to fermentor together.
Step 2: zymocyte liquid is accessed into fermentor and is fermented.Wherein, zymocyte liquid is 1:0.5 by concentration ratio:
0.1 streptococcus thermophilus, aspergillus niger, lactobacillus bulgaricus composition, wherein the concentration of streptococcus thermophilus is 4 × 107CFU/mL,
The additional amount of zymocyte liquid is the 0.5% of hickory chick quality.Keeping fermentation temperature is 30 DEG C, continuing fermentation 48h.
Step 3: filtering after having fermented obtains the first filtrate and the first filter residue, and the first filter residue is that insoluble diet is fine
Dimension.
Step 4: being concentrated into the 1/2 of original volume for the first filtrate, obtains concentration filtrate, is added 2 times into concentration filtrate
The ethyl alcohol of its volume, stands 1h after mixing evenly, and the second filtrate and the second filter residue is obtained by filtration；Second filter residue is that main component is
The nutriment of amino acid.
Step 5: the ethyl alcohol of 2 times of its volumes being added into the second filtrate, stands 1h after mixing evenly, is obtained by filtration the
Three filtrates and third filter residue；Third filtrate is soluble dietary fiber crude product.
Embodiment 2
The method that the present embodiment provides a kind of from hickory chick extracts dietary fiber, this method using microbial fermentation method from
Dietary fiber is extracted in hickory chick, can obtain soluble dietary fiber and insoluble diedairy fiber, and in isolated hickory chick
Other nutriments, while soluble dietary fibre content is high, physiological activity is high.This method includes the following steps:
Step 1: taking fresh hickory chick, clean up, and is that drinking water is added in 1:1.5 according to solid-liquid ratio, and 40min is boiled in heating,
After being cooled to room temperature, hickory chick and water are transferred to fermentor together.
Step 2: streptococcus thermophilus, aspergillus niger, lactobacillus bulgaricus strain are taken, each strain is seeded to solid respectively
LB culture medium activation 12h obtains activated seed, and activated seed is accessed culture 12h in LB liquid medium and obtains activation bacterium solution,
Activation bacterium solution is seeded in hickory chick solid medium again and cultivates 12h, the strain after being tamed.Wherein, hickory chick solid
Culture medium is made of common LB solid medium with hickory chick extracting solution according to volume ratio for 1:2, and hickory chick extracting solution is by such as
The preparation of lower section method: drinking water is added according to solid-liquid ratio for 1:1(g:L) in the hickory chick of fresh clean, 30min is boiled in heating, cold
But to slurrying after room temperature, take filtrate up to hickory chick extracting solution after crossing three layers of gauze.
Step 3: zymocyte liquid is accessed into fermentor and is fermented.Wherein, zymocyte liquid is by above-mentioned thermophilic after taming
Hot streptococcus, aspergillus niger, lactobacillus bulgaricus are according to concentration than the ratio composition for 1:0.8:0.3, wherein streptococcus thermophilus
Concentration be 8 × 107CFU/mL, the additional amount of zymocyte liquid are the 5% of hickory chick quality.In addition, sheep tripe is added into fermentor
The skimmed milk power of mycoplasma amount 3%, 0.3% glucose as extra-nutrition substance, keeping fermentation temperature is 45 DEG C, continuing fermentation
24h。
Step 4: filtering after having fermented obtains the first filtrate and the first filter residue, and the first filter residue is that insoluble diet is fine
Dimension.
Step 5: being concentrated into the 1/3 of original volume for the first filtrate, obtains concentration filtrate, is added 2 times into concentration filtrate
The ethyl alcohol of its volume, stands 1.5h after mixing evenly, and the second filtrate and the second filter residue is obtained by filtration；Second filter residue is main component
For the nutriment of amino acid.
Step 6: the ethyl alcohol of 2 times of its volumes being added into the second filtrate, stands 2h after mixing evenly, is obtained by filtration the
Three filtrates and third filter residue；Third filtrate is soluble dietary fiber crude product.
Embodiment 3
The method that the present embodiment provides a kind of from hickory chick extracts dietary fiber, this method using microbial fermentation method from
Dietary fiber is extracted in hickory chick, can obtain soluble dietary fiber and insoluble diedairy fiber, and in isolated hickory chick
Other nutriments, while soluble dietary fibre content is high, physiological activity is high.As shown in Figure 1, this method includes following step
It is rapid:
Step 1: taking fresh hickory chick, clean up, and is that drinking water is added in 1:1 according to solid-liquid ratio, 30min is boiled in heating, cold
But to after room temperature, hickory chick and water are transferred to fermentor together.
Step 2: streptococcus thermophilus, aspergillus niger, lactobacillus bulgaricus strain are taken, each strain is seeded to solid respectively
LB culture medium activation 16h obtains activated seed, and activated seed is accessed culture 8h in LB liquid medium and obtains activation bacterium solution, then
Activation bacterium solution is seeded in hickory chick solid medium and cultivates 16h, the strain after being tamed.Wherein, hickory chick solid is trained
It supports base to be made of common LB solid medium with hickory chick extracting solution according to volume ratio for 1:2, hickory chick extracting solution passes through as follows
Method preparation: drinking water is added according to solid-liquid ratio for 1:1(g:L) in the hickory chick of fresh clean, 30min is boiled in heating, cooling
Slurrying after to room temperature takes filtrate up to hickory chick extracting solution after crossing three layers of gauze.
Step 3: zymocyte liquid is accessed into fermentor and is fermented.Wherein, zymocyte liquid is by above-mentioned thermophilic after taming
Hot streptococcus, aspergillus niger, lactobacillus bulgaricus are according to concentration than the ratio composition for 1:0.6:0.2, wherein streptococcus thermophilus
Concentration be 5 × 107CFU/mL, the additional amount of zymocyte liquid are the 2% of hickory chick quality.In addition, sheep tripe is added into fermentor
The skimmed milk power of mycoplasma amount 2%, 0.4% glucose as extra-nutrition substance, keeping fermentation temperature is 35 DEG C, continuing fermentation
36h。
Step 4: filtering after having fermented obtains the first filtrate and the first filter residue, and the first filter residue is that insoluble diet is fine
Dimension.
Step 5: being concentrated into the 1/3 of original volume for the first filtrate, obtains concentration filtrate, is first added 4% into concentration filtrate
Sodium carbonate, adjust concentration filtrate pH be 6.8, then to concentration filtrate in be added 2 times of its volumes ethyl alcohol, after mixing evenly
1.5h is stood, the second filtrate and the second filter residue is obtained by filtration；Second filter residue is the nutriment that main component is amino acid.
Step 6: the ethyl alcohol of 2 times of its volumes is added into the second filtrate, stands 1.5h after mixing evenly, is obtained by filtration
Third filtrate and third filter residue；Third filtrate is soluble dietary fiber crude product.
Embodiment 4
The method that the present embodiment provides a kind of from hickory chick extracts dietary fiber, this method using microbial fermentation method from
Dietary fiber is extracted in hickory chick, can obtain soluble dietary fiber and insoluble diedairy fiber, and in isolated hickory chick
Other nutriments, while soluble dietary fibre content is high, physiological activity is high.As shown in Figure 1, this method includes following step
It is rapid:
Step 1: taking fresh hickory chick, clean up, and is that drinking water is added in 1:1 according to solid-liquid ratio, 30min is boiled in heating, cold
But to after room temperature, hickory chick and water are transferred to fermentor together.
Step 2: streptococcus thermophilus, aspergillus niger, lactobacillus bulgaricus strain are taken, each strain is seeded to solid respectively
LB culture medium activation 14h obtains activated seed, and activated seed is accessed culture 10h in LB liquid medium and obtains activation bacterium solution,
Activation bacterium solution is seeded in hickory chick solid medium again and cultivates 14h, the strain after being tamed.Wherein, hickory chick solid
Culture medium is made of common LB solid medium with hickory chick extracting solution according to volume ratio for 1:2, and hickory chick extracting solution is by such as
The preparation of lower section method: drinking water is added according to solid-liquid ratio for 1:1(g:L) in the hickory chick of fresh clean, 30min is boiled in heating, cold
But to slurrying after room temperature, take filtrate up to hickory chick extracting solution after crossing three layers of gauze.
Step 3: zymocyte liquid is accessed into fermentor and is fermented.Wherein, zymocyte liquid is by above-mentioned thermophilic after taming
Hot streptococcus, aspergillus niger, lactobacillus bulgaricus are according to concentration than the ratio composition for 1:0.7:0.3, wherein streptococcus thermophilus
Concentration be 6 × 107CFU/mL, the additional amount of zymocyte liquid are the 4% of hickory chick quality.In addition, sheep tripe is added into fermentor
The skimmed milk power of mycoplasma amount 1.5%, 0.45% glucose as extra-nutrition substance, keeping fermentation temperature is 40 DEG C, lasting to send out
Ferment 30h.
Step 4: filtering after having fermented obtains the first filtrate and the first filter residue, and the first filter residue is that insoluble diet is fine
Dimension.
Step 5: being concentrated into the 1/2 of original volume for the first filtrate, obtains concentration filtrate, is first added 4% into concentration filtrate
Sodium carbonate, adjust concentration filtrate pH be 7.4, then to concentration filtrate in be added 2 times of its volumes ethyl alcohol, after mixing evenly
2h is stood, the second filtrate and the second filter residue is obtained by filtration；Second filter residue is the nutriment that main component is amino acid.
Step 6: the ethyl alcohol of 2 times of its volumes being added into the second filtrate, stands 2h after mixing evenly, is obtained by filtration the
Three filtrates and third filter residue；Third filtrate is soluble dietary fiber crude product.
The invention is not limited to specific embodiments above-mentioned.The present invention, which expands to, any in the present specification to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (9)
1. it is a kind of from hickory chick extract dietary fiber method, which is characterized in that itself the following steps are included:
(1) take fresh hickory chick to clean, be 1:0.5-1.5(g:L according to solid-liquid ratio) drinking water is added, 20- is boiled in heating
40min；
(2) after being cooled to room temperature, hickory chick band water is transferred to fermentor together, and access zymocyte liquid sealing and fermenting；
(3) the first filtrate and the first filter residue is obtained by filtration；First filter residue is insoluble diedairy fiber crude product；
(4) 1/2-1/3 that the first filtrate to original volume is concentrated obtains concentration filtrate, and the ethyl alcohol of 2 times of concentration filtrate volumes is added, mixes
After even, 1-2h is stood, the second filtrate and the second filter residue is obtained by filtration；
(5) ethyl alcohol of 2 times of second filtrate volume is added into the second filtrate, after mixing, stands 1-2h, third filtrate is obtained by filtration
With third filter residue；Third filter residue is soluble dietary fiber crude product.
2. the method according to claim 1 for extracting dietary fiber from hickory chick, which is characterized in that, will in step (1)
Hickory chick after cleaning is crushed to granularity and boils to heat after the particle of 0.8-1.5cm.
3. the method according to claim 1 for extracting dietary fiber from hickory chick, which is characterized in that step (2) is described
Zymocyte liquid by concentration than being formed for the streptococcus thermophilus, aspergillus niger, lactobacillus bulgaricus of 1:0.5-0.8:0.1-0.3, it is thermophilic
The streptococcic concentration of heat is (4-8) × 107CFU/mL。
4. the method according to claim 3 for extracting dietary fiber from hickory chick, which is characterized in that zymocyte liquid adds
Enter the 0.5-5% that amount is hickory chick quality.
5. the method according to claim 4 for extracting dietary fiber from hickory chick, which is characterized in that add into fermentor
Enter the skimmed milk power of hickory chick quality 1-3%, the glucose of 0.3-0.5%.
6. the method according to claim 5 for extracting dietary fiber from hickory chick, which is characterized in that be sealed by fermentation and continue
24-48h, fermentation process temperature are 30-45 DEG C.
7. the method for dietary fiber is extracted from hickory chick according to any one of claim 2-6, which is characterized in that right
Each strain in the zymocyte liquid accesses fermentor after being tamed again；Domestication process includes: respectively to be seeded to each strain
Solid LB media activation 12-16h obtains activated seed, and activated seed is accessed culture 8-12h in LB liquid medium and is obtained
Bacterium solution is activated, then activation bacterium solution is seeded in hickory chick solid medium and cultivates 12-16h to get the strain after domestication.
8. the method according to claim 7 for extracting dietary fiber from hickory chick, which is characterized in that the training of hickory chick solid
It supports base to be made of common LB solid medium with hickory chick extracting solution according to volume ratio for 1:2, hickory chick extracting solution passes through as follows
Method preparation: drinking water is added according to solid-liquid ratio for 1:1(g:L) in fresh clean hickory chick, heating is boiled 30min, is cooled to
Slurrying after room temperature takes filtrate up to hickory chick extracting solution after crossing three layers of gauze.
9. the method according to claim 1 for extracting dietary fiber from hickory chick, which is characterized in that in step (4), first
4% sodium carbonate is added into concentration filtrate, the pH for adjusting concentration filtrate is 6.8-7.4, then adds the ethyl alcohol of 2 times of volumes.
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Application publication date: 20181207 |