CN108934784A - A kind of mushroom high-yield culturing culture medium - Google Patents
A kind of mushroom high-yield culturing culture medium Download PDFInfo
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- CN108934784A CN108934784A CN201811215638.1A CN201811215638A CN108934784A CN 108934784 A CN108934784 A CN 108934784A CN 201811215638 A CN201811215638 A CN 201811215638A CN 108934784 A CN108934784 A CN 108934784A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of mushroom high-yield culturing culture mediums, it is related to fungus growing technique reason, raw material includes: 35-45 parts of sawdust, 17-25 parts of stalk, 5-12 parts of corncob, 15-25 parts of wheat bran, 2-4 parts of quick lime, 1-2 parts of gypsum, 2-5 parts of eggshell, 5-10 parts of megasse, 1-3 parts of sustained-release synergistic agent, 5-10 parts of feed with paper-mulberry leaf, 0.5-1 parts of plant ash, 0.1-0.3 parts of urea by weight.Culture medium nutrition equilibrium of the invention is abundant, ventilative good water-retaining property, the problem of improving cultivation later period subalimentation, conducive to the growth of mushroom, to improve the yield and quality of mushroom, it is improved using the protein and total sugar content of the mushroom of culture medium cultivation, the mushroom meat cultivated is thick, taste is fragrant, mouthfeel cunning is tender palatable.
Description
Technical field
The present invention relates to technical field of edible fungi cultivation more particularly to a kind of mushroom high-yield culturing culture mediums.
Background technique
Mushroom is a kind of cultivation fungi of food and medicament dual-purpose in the civil title for being known as " mountain delicacy ".Traditional Chinese medical theory thinks fragrant
Mushroom nature and flavor are sweet, flat, cool, enter liver and kidney channel, have nourishing liver and kidney, strengthening the spleen and stomach, intelligence promoting and tranquilization, skin maintenance and cosmetic benefits and other effects, and contain in mushroom
The nutriments such as some lentinans, unsaturated fatty acid, double disulfides, ergosterol have and improve immunity, disease-resistant
The effect of poison, cancer preventing and treating, liver protecting, mushroom healthy nutritive value with higher.
China's cultivating champignon has a long history, and mushroom is ground in the attention with scientific and technological progress and people to health
Study carefully and develop it is increasingly deep, how to further increase the yield and quality of mushroom be research hot spot.Using the packed cultivation of culture medium
Mushroom is trained, there are bacteria stick nutrition early period excessively to concentrate, the disadvantage of later period nutrient deficiency, and is influenced by high temperature in canopy, culture medium water
Point fast easy dehydration is evaporated, therefore, researches and develops a kind of balanced training that can supplement release nutritional ingredient comprehensively and in the cultivation later period of nutrition
Based formulas is supported, guarantees that its quality is an important research topic again while improving mushroom production as far as possible.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of mushroom high-yield culturing culture medium, the trainings
It is abundant to support base nutrition equilibrium, good water-retaining property of breathing freely can improve the problem of cultivating later period subalimentation, conducive to the growth of mushroom.
A kind of mushroom high-yield culturing culture medium proposed by the present invention, raw material include: by weight 35-45 parts of sawdust,
17-25 parts of stalk, 5-12 parts of corncob, 15-25 parts of wheat bran, 2-4 parts of quick lime, 1-2 parts of gypsum, 2-5 parts of eggshell, megasse 5-
10 parts, 1-3 parts of sustained-release synergistic agent, 5-10 parts of feed with paper-mulberry leaf, 0.5-1 parts of plant ash, 0.1-0.3 parts of urea.
Preferably, the sustained-release synergistic agent prepare it is as follows: after montmorillonite is roasted ultrasonic disperse in water, be added silane
The ethanol solution of coupling agent, ultrasonic reaction filter, and wash, dry, obtain material a;Starch is added to the water and carries out water-bath gelatinization,
It is cooling, under ice-water bath, acrylic acid neutralizer, acrylamide and material a are added thereto, initiator and friendship is added in ultrasonic disperse
Join agent, stirring, heating in water bath for reaction, reaction product is precipitated with dehydrated alcohol, be dry, pulverize, and material b is obtained;By Radix Notoginseng medicine
Slag, feed with paper-mulberry leaf mixing, crush, add water, impregnate, and brown sugar and microbial bacteria I is added, then microbial bacteria II is added in stirring fermentation,
Anaerobic fermentation is sealed, is filtered, concentration obtains fermentation liquid c;Fermentation liquid is sprayed on material b, vibration, dry, obtained sustained-release synergistic
Agent;Cotton seed hulls, bagasse, corncob, wheat bran, corn flour, quick lime, calcium superphosphate, acid-sludge are mixed, heap fermentation, fermentation are built
After be added sustained-release synergistic agent, mix thoroughly, adjust water content, sterilize to get crab flavour mushroom culture medium.
Preferably, the sustained-release synergistic agent prepare it is as follows: will be added in 100-120 part water after the roasting of 7 parts of montmorillonites, it is super
Sound disperses 10-20min, and the ethanol solution of the silane coupling agent of 45-60 parts of 2.5-3.5%, ultrasonic reaction 20- are added thereto
40min is filtered, and is washed, dry, obtains material a;16-22 parts of starch are added in 150-200 parts of water, under nitrogen protection in 70-
Water-bath is gelatinized 20-30min at 80 DEG C, cooling, and under the conditions of ice-water bath, the 65- containing 10-15 parts of acrylic acid is added thereto
75% acrylic acid neutralizer, 20-27 parts of acrylamides and material a, ultrasonic disperse 20-30min are added 0.05-0.12 parts and draw
Send out agent and 0.04-0.1 parts of crosslinking agents, stirring, heating water bath to 60-70 DEG C of isothermal reaction 2-3h, reaction product dehydrated alcohol
It is precipitated, be dry, pulverize, obtain material b;The 12-16 parts of Radix Notoginseng dregs of a decoction are crushed, water is added, impregnate 40-60min, 0.08- is added
0.2 portion of brown sugar and 0.03-0.06 parts of microbial bacterias I, stirring fermentation 60-72h, is then added 0.01-0.03 parts at 30-35 DEG C
Microbial bacteria II seals anaerobic fermentation 24-48h at 25-30 DEG C, and filtering, concentrating the filtrate to relative density is 0.99-
1.03, obtain fermentation liquid c;Fermentation liquid c is sprayed on material b, vibration, drying are to get sustained-release synergistic agent.
Preferably, the temperature of the montmorillonite roasting is 600-700 DEG C, calcining time 1-2h.
Preferably, the silane coupling agent is KH550.
Preferably, the microbial bacteria I includes bacillus subtilis, aspergillus niger, lactobacillus plantarum, bacillus bulgaricus,
Microbial bacteria II is lactobacillus plantarum.
Preferably, the bacillus subtilis, aspergillus niger, lactobacillus plantarum, bacillus bulgaricus weight ratio be 2-3:
1-2:2-3:1-2.
Preferably, the sawdust is broad-leaf forest sawdust.
The preparation of above-mentioned mushroom high-yield culturing culture medium is prepared according to the conventional method of the prior art.
The utility model has the advantages that being with sawdust, stalk, corncob, wheat the invention discloses a kind of mushroom high-yield culturing culture medium
Bran, quick lime, gypsum, eggshell, megasse, feed with paper-mulberry leaf, plant ash, urea, sustained-release synergistic agent are raw material, wherein in megasse
Residual to have a certain amount of sugar and a large amount of crude fibre, protein content is higher in feed with paper-mulberry leaf, amino acid also rich in, dimension
Raw element, carbohydrate and microelement, plant ash can not only provide nutrient for mycelia, moreover it is possible to inhibit varied bacteria growing, there is heat preservation
Promote the effect of mycelia growth, the above ingredient is matched with sawdust, stalk, corncob, wheat bran, quick lime, gypsum, eggshell, is sought
Support equilibrium comprehensively, nutrition needed for being improved for mushroom growth, and mycelia growth and fruiting can be promoted, and the sustained-release synergistic agent energy added
It is enough that nutritional ingredient is slowly persistently discharged in the cultivation of mushroom, nutrient is supplemented for the cultivation later period, to improve entire cultivate
The mushroom production and quality in stage, the mushroom meat cultivated is thick, taste is fragrant, mouthfeel cunning is tender palatable.
The preparation of sustained-release synergistic agent in the present invention is that have preferable using the montmorillonite with preferable adsorption capacity as raw material
Gas permeability, reacted after being fired with silane coupling agent, under the action of ultrasound is with ethanol medium, silane coupling agent
On the one hand it is condensed with coupling mode on the surface and edge of montmorillonite, the interlayer of montmorillonite is on the other hand inserted into, to change
The surface nature of montmorillonite improves its dispersibility in high molecular polymer, then participates in propylene for montmorillonite as raw material
In the graft copolymerization of acid, acrylamide and starch, chemical reaction or physical crosslinking occurs with macromolecule network, is conducive to it
The raising of water absorbing properties, high molecular polymer surface obtained are dispersed with a large amount of holes and coarse gauffer, there is good absorption
Effect;Also contain a variety of active ingredients in the Radix Notoginseng dregs of a decoction, by it after microbial composite bacteria stirring fermentation and anaerobic fermentation, makes it
In nutritional ingredient and active functional component release, be easy to be absorbed by mycelia, promote its growth, fermentation liquid is sprayed and is adsorbed
On the surface and inside of high molecular polymer, slow release nutrient, makes up cultivation later period nutrient deficiency, fertilising in the course of cultivation
Inconvenient defect.The sustained-release synergistic agent is added in culture medium, can be improved the ventilative water holding capacity of culture medium, improves cultivation
The problem of later period subalimentation, conducive to the growth of mushroom, so that the yield and quality of mushroom is improved, using culture medium cultivation
The protein and total sugar content of mushroom improve.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A kind of mushroom high-yield culturing culture medium proposed by the present invention, raw material include: broad-leaf forest sawdust 35 by weight
Part, 25 parts of stalk, 5 parts of corncob, 25 parts of wheat bran, 2 parts of quick lime, 1 part of gypsum, 2 parts of eggshell, 5 parts of megasse, sustained-release synergistic agent
1 part, 10 parts of feed with paper-mulberry leaf, 0.5 part of plant ash, 0.3 part of urea;
Wherein, the sustained-release synergistic agent prepare it is as follows: 7 parts of montmorillonites are roasted into 1h at 600 DEG C, are then added 100
In part water, the ethanol solution of 45 part 2.5% of Silane coupling agent KH550, ultrasonic reaction is added in ultrasonic disperse 10min thereto
20min is filtered, and is washed, dry, obtains material a;16 parts of starch are added in 150 parts of water, the water-bath at 70 DEG C under nitrogen protection
Be gelatinized 20min, it is cooling, under the conditions of ice-water bath, be added thereto 65% containing 10 parts of acrylic acid acrylic acid neutralizer, 27
Part acrylamide and material a, ultrasonic disperse 20min, are added 0.05 part of initiator and 0.04 part of crosslinking agent, stir, heating water bath
To 60 DEG C of isothermal reaction 2h, reaction product is precipitated with dehydrated alcohol, be dry, pulverize, and obtains material b;By 12 parts of Radix Notoginseng dregs of a decoction
It crushes, adds water, impregnate 40min, 0.08 portion of brown sugar and 0.03 part of microbial bacteria I is added, which is by bacillus subtilis
Bacterium, aspergillus niger, lactobacillus plantarum, bacillus bulgaricus are formed according to the weight ratio compounding of 2:2:2:2, and hair is stirred at 30 DEG C
Then 0.01 part of II lactobacillus plantarum of microbial bacteria is added in ferment 60h, seal anaerobic fermentation for 24 hours at 25 DEG C, filtering, by filtrate
Being concentrated into relative density is 0.99 (60 DEG C of surveys), obtains fermentation liquid c;Fermentation liquid c is sprayed on material b, vibration, it is dry to get
Sustained-release synergistic agent.
Above-mentioned mushroom high-yield culturing is prepared with culture medium according to conventional method in the prior art, and specific preparation is such as
Under: broad-leaf forest sawdust, stalk, corncob, wheat bran, quick lime, gypsum, eggshell, megasse, feed with paper-mulberry leaf, plant ash, urea are mixed
Close, adjust water content, build heap fermentation, after fermentation be added sustained-release synergistic agent, mix thoroughly, adjust water content, sterilizing to get.
Embodiment 2
A kind of mushroom high-yield culturing culture medium proposed by the present invention, raw material include: broad-leaf forest sawdust 45 by weight
Part, 17 parts of stalk, 12 parts of corncob, 15 parts of wheat bran, 4 parts of quick lime, 2 parts of gypsum, 5 parts of eggshell, 10 parts of megasse, sustained-release synergistic
3 parts of agent, 5 parts of feed with paper-mulberry leaf, 1 part of plant ash, 0.1 part of urea;
Wherein, the sustained-release synergistic agent prepare it is as follows: 7 parts of montmorillonites are roasted into 2h at 700 DEG C, are then added 120
In part water, the ethanol solution of 60 part 3.5% of Silane coupling agent KH550, ultrasonic reaction is added in ultrasonic disperse 20min thereto
40min is filtered, and is washed, dry, obtains material a;22 parts of starch are added in 200 parts of water, the water-bath at 80 DEG C under nitrogen protection
Be gelatinized 30min, it is cooling, under the conditions of ice-water bath, be added thereto 75% containing 15 parts of acrylic acid acrylic acid neutralizer, 20
Part acrylamide and material a, ultrasonic disperse 30min, are added 0.12 part of initiator and 0.1 part of crosslinking agent, stir, heating water bath is extremely
70 DEG C of isothermal reaction 3h, reaction product are precipitated with dehydrated alcohol, be dry, pulverize, and material b is obtained;By 16 parts of Radix Notoginseng dregs of a decoction powder
It is broken, add water, impregnate 60min, 0.2 portion of brown sugar and 0.06 part of microbial bacteria I is added, the microbial bacteria I be by bacillus subtilis,
Aspergillus niger, lactobacillus plantarum, bacillus bulgaricus are formed according to the weight ratio compounding of 3:1:3:1, and fermentation is stirred at 35 DEG C
Then 0.03 part of II lactobacillus plantarum of microbial bacteria is added in 72h, anaerobic fermentation 48h is sealed at 30 DEG C, and filtering is dense by filtrate
Being reduced to relative density is 1.03 (60 DEG C of surveys), obtains fermentation liquid c;Fermentation liquid c is sprayed on material b, vibration, drying are to get slow
Release synergist.
Above-mentioned mushroom high-yield culturing is prepared with culture medium according to conventional method in the prior art, and specific preparation is such as
Under: broad-leaf forest sawdust, stalk, corncob, wheat bran, quick lime, gypsum, eggshell, megasse, feed with paper-mulberry leaf, plant ash, urea are mixed
Close, adjust water content, build heap fermentation, after fermentation be added sustained-release synergistic agent, mix thoroughly, adjust water content, sterilizing to get.
Embodiment 3
A kind of mushroom high-yield culturing culture medium proposed by the present invention, raw material include: broad-leaf forest sawdust 42 by weight
Part, 20 parts of stalk, 7 parts of corncob, 22 parts of wheat bran, 2.5 parts of quick lime, 1.5 parts of gypsum, 4 parts of eggshell, 8 parts of megasse, sustained release increase
Imitate 1.5 parts of agent, 6 parts of feed with paper-mulberry leaf, 0.8 part of plant ash, 0.2 part of urea;
Wherein, the sustained-release synergistic agent prepare it is as follows: 7 parts of montmorillonites are roasted into 1.5h at 640 DEG C, are then added
In 105 parts of water, the ethanol solution of 52 part 3% of Silane coupling agent KH550, ultrasonic reaction is added in ultrasonic disperse 15min thereto
25min is filtered, and is washed, dry, obtains material a;18 parts of starch are added in 180 parts of water, the water-bath at 75 DEG C under nitrogen protection
Be gelatinized 25min, it is cooling, under the conditions of ice-water bath, be added thereto 70% containing 12 parts of acrylic acid acrylic acid neutralizer, 25
Part acrylamide and material a, ultrasonic disperse 25min, are added 0.08 part of initiator and 0.06 part of crosslinking agent, stir, heating water bath
To 65 DEG C of isothermal reaction 2.5h, reaction product is precipitated with dehydrated alcohol, be dry, pulverize, and obtains material b;By 13 parts of Radix Notoginseng medicines
Ground-slag is broken, adds water, impregnates 50min, 0.1 portion of brown sugar and 0.04 part of microbial bacteria I is added, which is by withered grass gemma
Bacillus, aspergillus niger, lactobacillus plantarum, bacillus bulgaricus are formed according to the weight ratio compounding of 2.6:1.4:2.8:1.3,32
Stirring fermentation 64h, is then added 0.02 part of II lactobacillus plantarum of microbial bacteria at DEG C, and anaerobic fermentation 36h, mistake are sealed at 27 DEG C
Filter, concentrating the filtrate to relative density is 1.00 (60 DEG C of surveys), obtains fermentation liquid c;Fermentation liquid c is sprayed on material b, vibration,
Drying is to get sustained-release synergistic agent.
Above-mentioned mushroom high-yield culturing is prepared with culture medium according to conventional method in the prior art, and specific preparation is such as
Under: broad-leaf forest sawdust, stalk, corncob, wheat bran, quick lime, gypsum, eggshell, megasse, feed with paper-mulberry leaf, plant ash, urea are mixed
Close, adjust water content, build heap fermentation, after fermentation be added sustained-release synergistic agent, mix thoroughly, adjust water content, sterilizing to get.
Embodiment 4
A kind of mushroom high-yield culturing culture medium proposed by the present invention, raw material include: broad-leaf forest sawdust 40 by weight
Part, 22 parts of stalk, 10 parts of corncob, 20 parts of wheat bran, 3 parts of quick lime, 1.5 parts of gypsum, 3 parts of eggshell, 7 parts of megasse, sustained release increase
Imitate 2 parts of agent, 8 parts of feed with paper-mulberry leaf, 0.6 part of plant ash, 0.2 part of urea;
Wherein, the sustained-release synergistic agent prepare it is as follows: 7 parts of montmorillonites are roasted into 1.5h at 680 DEG C, are then added
In 110 parts of water, the ethanol solution of 55 part 3% of Silane coupling agent KH550, ultrasonic reaction is added in ultrasonic disperse 10min thereto
35min is filtered, and is washed, dry, obtains material a;20 parts of starch are added in 180 parts of water, the water-bath at 70 DEG C under nitrogen protection
Be gelatinized 30min, it is cooling, under the conditions of ice-water bath, be added thereto 75% containing 14 parts of acrylic acid acrylic acid neutralizer, 23
Part acrylamide and material a, ultrasonic disperse 20min, are added 0.07 part of initiator and 0.05 part of crosslinking agent, stir, heating water bath
To 70 DEG C of isothermal reaction 2.5h, reaction product is precipitated with dehydrated alcohol, be dry, pulverize, and obtains material b;By 15 parts of Radix Notoginseng medicines
Ground-slag is broken, adds water, impregnates 60min, 0.15 portion of brown sugar and 0.05 part of microbial bacteria I is added, which is by withered grass gemma
Bacillus, aspergillus niger, lactobacillus plantarum, bacillus bulgaricus are formed according to the weight ratio compounding of 2.5:1.5:2.5:1.5,34
Stirring fermentation 68h, is then added 0.03 part of II lactobacillus plantarum of microbial bacteria at DEG C, and anaerobic fermentation 42h, mistake are sealed at 28 DEG C
Filter, concentrating the filtrate to relative density is 1.02 (60 DEG C of surveys), obtains fermentation liquid c;Fermentation liquid c is sprayed on material b, vibration,
Drying is to get sustained-release synergistic agent.
Above-mentioned mushroom high-yield culturing is prepared with culture medium according to conventional method in the prior art, and specific preparation is such as
Under: broad-leaf forest sawdust, stalk, corncob, wheat bran, quick lime, gypsum, eggshell, megasse, feed with paper-mulberry leaf, plant ash, urea are mixed
Close, adjust water content, build heap fermentation, after fermentation be added sustained-release synergistic agent, mix thoroughly, adjust water content, sterilizing to get.
Comparative example 1
Compared to embodiment 4, difference is only that in culture medium raw material that sustained-release synergistic agent is montmorillonite, remaining is all the same,
Preparation method is also identical.
The culture medium prepared in embodiment 4 and comparative example 1 is used for cultivating champignon, the influence factors item such as daily management condition
Part is all consistent, tests nutritional ingredient in mushroom, through detecting, compared with comparative example 1, and the crude protein of the mushroom produced in embodiment 4
28.5% is improved, crude fat improves 14.5%, and total reducing sugar improves 12.8%, and nutritive value significantly improves, and total output mentions
It is high by 68.2%.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of mushroom high-yield culturing culture medium, which is characterized in that its raw material includes: 35-45 parts of sawdust, stalk by weight
17-25 parts, 5-12 parts of corncob, 15-25 parts of wheat bran, 2-4 parts of quick lime, 1-2 parts of gypsum, 2-5 parts of eggshell, megasse 5-10
Part, 1-3 parts of sustained-release synergistic agent, 5-10 parts of feed with paper-mulberry leaf, 0.5-1 parts of plant ash, 0.1-0.3 parts of urea.
2. mushroom high-yield culturing culture medium according to claim 1, which is characterized in that the preparation of the sustained-release synergistic agent
As follows: the ethanol solution of silane coupling agent, ultrasonic reaction is added in water in ultrasonic disperse after montmorillonite is roasted, and filtering is washed
It washs, it is dry, obtain material a;Starch is added to the water and carries out water-bath gelatinization, it is cooling, under ice-water bath, acrylic acid is added thereto and neutralizes
Initiator and crosslinking agent, stirring, heating in water bath for reaction, reaction product nothing is added in liquid, acrylamide and material a, ultrasonic disperse
Water-ethanol is precipitated, and dry, pulverize, and obtains material b;The Radix Notoginseng dregs of a decoction, feed with paper-mulberry leaf are mixed, crushes, adds water, is impregnated, is added red
Sugar and microbial bacteria I, stirring fermentation, are then added microbial bacteria II, seal anaerobic fermentation, filter, and concentration obtains fermentation liquid c;It will
Fermentation liquid is sprayed on material b, vibration, dry, obtained sustained-release synergistic agent;By cotton seed hulls, bagasse, corncob, wheat bran, corn
Powder, quick lime, calcium superphosphate, acid-sludge mixing, build heap fermentation, sustained-release synergistic agent are added after fermentation, mixes thoroughly, adjust aqueous
Amount sterilizes to get crab flavour mushroom culture medium.
3. mushroom high-yield culturing culture medium according to claim 1 or 2, which is characterized in that the sustained-release synergistic agent
It prepares as follows: being added in 100-120 parts of water after 7 parts of montmorillonites are roasted, 45-60 is added in ultrasonic disperse 10-20min thereto
The ethanol solution of the silane coupling agent of part 2.5-3.5%, ultrasonic reaction 20-40min are filtered, and are washed, dry, obtain material a;It will
16-22 parts of starch are added in 150-200 parts of water, and water-bath is gelatinized 20-30min at 70-80 DEG C under nitrogen protection, cooling,
Under the conditions of ice-water bath, acrylic acid neutralizer, the 20-27 parts of acryloyls of the 65-75% containing 10-15 parts of acrylic acid are added thereto
0.05-0.12 parts of initiators and 0.04-0.1 parts of crosslinking agents, stirring, water-bath is added in amine and material a, ultrasonic disperse 20-30min
It is heated to 60-70 DEG C of isothermal reaction 2-3h, reaction product is precipitated with dehydrated alcohol, be dry, pulverize, and material b is obtained;By 12-
16 parts of Radix Notoginseng dregs of a decoction crush, and add water, impregnate 40-60min, and 0.08-0.2 portions of brown sugar and 0.03-0.06 parts of microbial bacterias I are added,
Stirring fermentation 60-72h, is then added 0.01-0.03 parts of microbial bacterias II at 30-35 DEG C, and anaerobism hair is sealed at 25-30 DEG C
Ferment 24-48h, filtering, concentrating the filtrate to relative density is 0.99-1.03, obtains fermentation liquid c;Fermentation liquid c is sprayed onto material b
On, vibration, drying are to get sustained-release synergistic agent.
4. mushroom high-yield culturing culture medium according to claim 1-3, which is characterized in that the montmorillonite roasting
The temperature of burning is 600-700 DEG C, calcining time 1-2h.
5. mushroom high-yield culturing culture medium according to claim 1-4, which is characterized in that described silane coupled
Agent is KH550.
6. mushroom high-yield culturing culture medium according to claim 1-5, which is characterized in that the microbial bacteria
I includes bacillus subtilis, aspergillus niger, lactobacillus plantarum, bacillus bulgaricus, and microbial bacteria II is lactobacillus plantarum.
7. mushroom high-yield culturing culture medium according to claim 6, which is characterized in that the bacillus subtilis, black
Aspergillus, lactobacillus plantarum, bacillus bulgaricus weight ratio be 2-3:1-2:2-3:1-2.
8. mushroom high-yield culturing culture medium according to claim 1-7, which is characterized in that the sawdust is wealthy
Leaf forest bits.
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CN109548562A (en) * | 2019-01-18 | 2019-04-02 | 上海国森生物科技有限公司 | A kind of mushroom cultivating method and cultivation matrix |
CN111972213A (en) * | 2020-09-09 | 2020-11-24 | 岳西县思远生态农业有限公司 | Black fungus cultivation method |
CN111972213B (en) * | 2020-09-09 | 2022-04-15 | 岳西县思远生态农业有限公司 | Black fungus cultivation method |
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Application publication date: 20181207 |