CN108934726A - A kind of planting yew method based on plantation of collecting seed - Google Patents

Abstract

The present invention provides a kind of planting yew method based on plantation of collecting seed, comprising steps of S1, selection plantation environment: the selection gradient less than 20 °, soil layer is deep, short texture, more, the agricultural land soil of excellent drainage containing humus；S2, seed collecting processing: going the ripening fruits on mature Chinese yew, is put into basin and is washed by rubbing with the hands to kind of a skin rupture, kernel stops when leaking out 50%, air-dries in shady spot spare；S3, seedbed arrange: digging pit, cheat deep 25~35mm, hole diameter is 35~45mm, 2.0~2.5m of adjacent pits spacing；S4, plantation: building greenhouse, seed is carried out program request into the hole dug, fine earth lid kind thickness 1-2cm sprinkles profoundly water, covers agricultural film, four side soil compactions, while poison bait also is made with zinc phosphide to prevent the plague of rats；S5, seedling replanting: when seedling grows into 3-4 leaf, transplanting final singling is carried out by spacing in the rows 15-20cm, line-spacing 25-30cm；S6, tending management；S7, transplanting afforestation: after seedling length to 30-50cm, transplanting afforestation is carried out in spring；S8, woodland nursing.

Description

A kind of planting yew method based on plantation of collecting seed

Technical field

The present invention relates to planting yew technical fields, more particularly to a kind of planting yew side based on plantation of collecting seed Method.

Background technique

Chinese yew belongs to shallow rooted plant, and main root is unobvious, lateral root is flourishing, is generally acknowledged endangered natural in the world Rare plants for anticancer is the ancient tree species that have passed through quaternary glacier and carry over, and has 2,500,000 years history on earth, Since growth of taxol speed is slow under field conditions (factors), power of regeneration is poor, so not had also in world wide since some time There is the Chinese yew raw material forestbase to be formed and advise touch greatly, Chinese yew is used as medicine with stem, branch, leaf, root, contains taxol, taxine, diterpenoids Compound has anti-cancer function, and has and inhibit diabetes and the cardiopathic effectiveness for the treatment of, since the growth cycle of Chinese yew is general It was acted at 8 years~10 years, it is low additionally, due to the survival rate of Chinese yew sapling, lead to deficiency in economic performance, peasant planting Chinese yew Enthusiasm it is not high.

Summary of the invention

In view of the foregoing deficiencies of prior art, the purpose of the present invention is to provide a kind of red beans based on plantation of collecting seed China fir implantation methods, the growth cycle for solving Chinese yew in the prior art is long, and the survival rate of Chinese yew sapling is low, cause through It helps problem of poor benefits.

The present invention provides a kind of planting yew method based on plantation of collecting seed, comprising steps of S1, selection plantation environment: Select the gradient less than 20 °, soil layer is deep, short texture, more, the agricultural land soil of excellent drainage containing humus；S2, seed collecting processing: The ripening fruits on mature Chinese yew is gone, is put into basin and is washed by rubbing with the hands to kind of a skin rupture, kernel stops when leaking out 50%, carrying on the back Negative place air-dries spare；S3, seedbed arrange: it digs pit, cheats deep 25~35mm, hole diameter is 35~45mm, adjacent pits spacing 2.0~ 2.5m；S4, plantation: building greenhouse, seed is carried out program request into the hole dug, fine earth lid kind thickness 1-2cm is sprinkled profoundly water, and is covered Upper agricultural film, four side soil compactions, while poison bait also is made with zinc phosphide prevents the plague of rats；S5, seedling replanting: it is grown to seedling When to 3-4 leaf, transplanting final singling is carried out by spacing in the rows 15-20cm, line-spacing 25-30cm；S6, tending management: if being found after field planting 1 week dead Strain is filled in time, is applied 1 nutrient solution after surviving weekly, is subject to and tree root is irrigated, and timing fertilising is timely loosened the soil and weeding, Focus on draining, prevents seedling from being impregnated by ponding, cause seedling by high temperature injury or death；S7, transplanting afforestation: long extremely in seedling After 30-50cm, transplanting afforestation is carried out in spring；S8, woodland nursing: 5~June of First Year and 8~September, which are respectively uprooted, after afforestation comforts 1 It is secondary, it should be earthed up to nursery stock when hoe is comforted, expand cave, bury blueness, to increase soil fertility, water moisture self restraint, since the 2nd year, into Row fertilising, at least 1~2 time every year, fertilizing method is to dig annular ditch in base portion, after compound fertilizer or farmyard manure uniformly applied into ditch Interior, every plant of 180-220g or so waters, then earthing is filled and led up, annual 7,8, September heat and drought condition season, soil drought water shortage Shi Bixu is irrigated in time, keeps furrow face ground moistening.

In one embodiment of the invention, the step S3 is comprising steps of carry out disinfection processing to seedbed, at the disinfection Reason is that every 667m2 zineb or 75% pentachloronitrobenzene 2kg puddle 40-60kg fine earth and be spread among seedbed.

In one embodiment of the invention, the step S4 is comprising steps of carry out seed soaking before sowing；At the seed soaking Reason is asks whose seed soaking 30 minutes with 25-35 °, then with the gibberellin liquid of 500ml/kg immersion 24-48 hours.

In one embodiment of the invention, nutrient solution described in step S6, the method for being prepared by the following procedure is obtained: S61, raw material Processing: the fresh complete stool of Chinese yew or fresh branches and leaves being cleaned up, dries, is crushed with pulverizer, crushed with 10mm mesh pulverizer, powder Particle degree is 2-10mm, is then placed in pressure pan, and the pure water of the .3 times of parts by weight of addition 0 is forced into 4kgf/cm2, makes it Pressure is uniform, and keeps 15min, momentary recovery normal pressure；S62, extraction: the crushing raw material that the step S61 is obtained is added 2 times The methanol heating extraction of weight, 45 DEG C are extracted 2 times, each 2h, filtering, merge concentrate, concentration, and temperature controls≤50 DEG C, very Sky control is concentrated into 0 .95-1 .05g/ml in ± 0 .01MPa of -0 .08, obtains concentrated extracting solution, detects content of taxol； S63, aqueous two-phase extraction: to the step S62, medicinal extract progress aqueous two-phase extraction is obtained, medicinal extract and aqueous two-phase extraction liquid are according to 1:1 .25 volume ratio obtains the upper phase of extraction and extracts lower phase, and double-aqueous phase system used in aqueous two-phase extraction is by polyethylene glycol, chlorine Change sodium and water is constituted, mass percent of the polyethylene glycol in the double-aqueous phase system is 15%, and sodium chloride is in the aqueous two-phase Mass percent in system is 15%, and surplus is water, and the weight average molecular weight of the polyethylene glycol is 8000；S64, by the step The aqueous phase extracted that rapid S63 is obtained, uses molecular cut off for the ultrafiltration membrane desalination of 1000-100000, obtains.

As described above, a kind of planting yew method based on plantation of collecting seed of the invention, has the advantages that

Growth cycle is shortened, survival rate is provided, and then improves the economic benefit of Chinese yew.

Specific embodiment

Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from Various modifications or alterations are carried out under spirit of the invention.It should be noted that in the absence of conflict, following embodiment and implementation Feature in example can be combined with each other.

It should be noted that illustrating the basic structure that only the invention is illustrated in a schematic way provided in following embodiment Think, only shown in schema then with related component in the present invention rather than component count, shape and size when according to actual implementation Draw, when actual implementation kenel, quantity and the ratio of each component can arbitrarily change for one kind, and its assembly layout kenel It is likely more complexity.

The present invention provides a kind of planting yew methods based on plantation of collecting seed, comprising steps of

S1, selection plantation environment: the selection gradient less than 20 °, soil layer is deep, short texture, more, the agriculture of excellent drainage containing humus Field soil；

S2, seed collecting processing: going the ripening fruits on mature Chinese yew, is put into basin and is washed by rubbing with the hands to kind of a skin rupture, kernel leakage Out 50% when stop, being air-dried in shady spot spare；

S3, seedbed arrange: digging pit, cheat deep 25~35mm, hole diameter is 35~45mm, 2.0~2.5m of adjacent pits spacing；

S4, plantation: building greenhouse, seed is carried out program request into the hole dug, fine earth lid kind thickness 1-2cm is sprinkled profoundly water, covered Agricultural film, four side soil compactions, while poison bait also is made with zinc phosphide prevents the plague of rats；

S5, seedling replanting: when seedling grows into 3-4 leaf, transplanting final singling is carried out by spacing in the rows 15-20cm, line-spacing 25-30cm；

S6, tending management: if field planting found that dead strain is filled in time after 1 week, 1 nutrient solution is applied after surviving, weekly tree root is poured Subject to thoroughly, timing is applied fertilizer, and timely loosens the soil and weeding, emphasis draining prevent seedling from being impregnated by ponding, cause seedling by hyperthermal injury It is harmful or dead；

S7, transplanting afforestation: after seedling length to 30-50cm, transplanting afforestation is carried out in spring；

S8, woodland nursing: 5~June of First Year and 8~September, which are respectively uprooted, after afforestation comforts 1 time, should train to nursery stock when hoe is comforted Soil expands cave, buries blueness, and to increase soil fertility, water moisture self restraint applied fertilizer since the 2nd year, and at least 1~2 time every year, fertilising Method be base portion dig annular ditch, after compound fertilizer or farmyard manure are uniformly applied in ditch, every plant of 180-220g or so, watering, then Earthing is filled and led up, annual 7,8, September heat and drought condition season, and when soil drought water shortage must irrigate in time, keeps furrow face soil It is wet.

In one embodiment of this invention, the step S3 is comprising steps of carry out disinfection processing to seedbed, at the disinfection Reason is that every 667m2 zineb or 75% pentachloronitrobenzene 2kg puddle 40-60kg fine earth and be spread among seedbed.

Further, the step S4 is comprising steps of carry out seed soaking before sowing；The seed soaking is with 25-35 ° Water seed soaking 30 minutes, then impregnated 24-48 hours with the gibberellin liquid of 500ml/kg.

In a preferred embodiment, nutrient solution described in step S6, the method for being prepared by the following procedure obtain:

S61, feedstock processing: the fresh complete stool of Chinese yew or fresh branches and leaves being cleaned up, dries, is crushed with pulverizer, with 10mm mesh Pulverizer crushes, and grinding particle size 2-10mm is then placed in pressure pan, and the pure water of the .3 times of parts by weight of addition 0 is forced into 4kgf/cm2 keeps its pressure uniform, and keeps 15min, momentary recovery normal pressure；

S62, extraction: the methanol heating extraction for crushing raw material and 2 times of weight being added that the step S61 is obtained, 45 DEG C are extracted 2 Secondary, each 2h, filtering merges concentrate, concentration, and temperature controls≤50 DEG C, and vacuum control is in ± 0 .01MPa of -0 .08, concentration To 0 .95-1 .05g/ml, concentrated extracting solution is obtained, detects content of taxol；

S63, aqueous two-phase extraction: to the step S62, medicinal extract progress aqueous two-phase extraction is obtained, medicinal extract and aqueous two-phase extraction liquid are pressed According to the volume ratio of 1:1 .25, obtains the upper phase of extraction and extract lower phase, double-aqueous phase system used in aqueous two-phase extraction is by poly- second two Alcohol, sodium chloride and water are constituted, and mass percent of the polyethylene glycol in the double-aqueous phase system is 15%, and sodium chloride is described double Mass percent in aqueous phase system is 15%, and surplus is water, and the weight average molecular weight of the polyethylene glycol is 8000；

S64, the aqueous phase extracted for obtaining the step S63, use molecular cut off for the ultrafiltration membrane desalination of 1000-100000, It obtains.

In conclusion the planting yew method of the invention based on plantation of collecting seed, shortens growth cycle, provides into Motility rate, and then improve the economic benefit of Chinese yew.So the present invention effectively overcomes various shortcoming in the prior art and has High industrial utilization value.

The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as At all equivalent modifications or change, should be covered by the claims of the present invention.

Claims (4)

1. a kind of planting yew method based on plantation of collecting seed, which is characterized in that comprising steps of

S1, selection plantation environment: the selection gradient less than 20 °, soil layer is deep, short texture, more, the agriculture of excellent drainage containing humus Field soil；

S2, seed collecting processing: going the ripening fruits on mature Chinese yew, is put into basin and is washed by rubbing with the hands to kind of a skin rupture, kernel leakage Out 50% when stop, being air-dried in shady spot spare；

S3, seedbed arrange: digging pit, cheat deep 25~35mm, hole diameter is 35~45mm, 2.0~2.5m of adjacent pits spacing；

S4, plantation: building greenhouse, seed is carried out program request into the hole dug, fine earth lid kind thickness 1-2cm is sprinkled profoundly water, covered Agricultural film, four side soil compactions, while poison bait also is made with zinc phosphide prevents the plague of rats；

S5, seedling replanting: when seedling grows into 3-4 leaf, transplanting final singling is carried out by spacing in the rows 15-20cm, line-spacing 25-30cm；

S6, tending management: if field planting found that dead strain is filled in time after 1 week, 1 nutrient solution is applied after surviving, weekly tree root is poured Subject to thoroughly, timing is applied fertilizer, and timely loosens the soil and weeding, emphasis draining prevent seedling from being impregnated by ponding, cause seedling by hyperthermal injury It is harmful or dead；

S7, transplanting afforestation: after seedling length to 30-50cm, transplanting afforestation is carried out in spring；

S8, woodland nursing: 5~June of First Year and 8~September, which are respectively uprooted, after afforestation comforts 1 time, should train to nursery stock when hoe is comforted Soil expands cave, buries blueness, and to increase soil fertility, water moisture self restraint applied fertilizer since the 2nd year, and at least 1~2 time every year, fertilising Method be base portion dig annular ditch, after compound fertilizer or farmyard manure are uniformly applied in ditch, every plant of 180-220g or so, watering, then Earthing is filled and led up, annual 7,8, September heat and drought condition season, and when soil drought water shortage must irrigate in time, keeps furrow face soil It is wet.

2. the planting yew method according to claim 1 based on plantation of collecting seed, which is characterized in that the step S3 packet It includes step: carrying out disinfection processing to seedbed, the disinfection treatment is that every 667m2 zineb or 75% pentachloronitrobenzene 2kg are puddled 40-60kg fine earth is spread among seedbed.

3. the planting yew method according to claim 1 based on plantation of collecting seed, which is characterized in that the step S4 packet It includes step: carrying out seed soaking before sowing；The seed soaking is to ask whose seed soaking 30 minutes with 25-35 °, then uses 500ml/kg Gibberellin liquid impregnate 24-48 hours.

4. the planting yew method according to claim 1 based on plantation of collecting seed, which is characterized in that described in step S6 Nutrient solution, the method for being prepared by the following procedure obtain:

S61, feedstock processing: the fresh complete stool of Chinese yew or fresh branches and leaves being cleaned up, dries, is crushed with pulverizer, with 10mm mesh Pulverizer crushes, and grinding particle size 2-10mm is then placed in pressure pan, and the pure water of the .3 times of parts by weight of addition 0 is forced into 4kgf/cm2 keeps its pressure uniform, and keeps 15min, momentary recovery normal pressure；

S62, extraction: the methanol heating extraction for crushing raw material and 2 times of weight being added that the step S61 is obtained, 45 DEG C are extracted 2 Secondary, each 2h, filtering merges concentrate, concentration, and temperature controls≤50 DEG C, and vacuum control is in ± 0 .01MPa of -0 .08, concentration To 0 .95-1 .05g/ml, concentrated extracting solution is obtained, detects content of taxol；

S63, aqueous two-phase extraction: to the step S62, medicinal extract progress aqueous two-phase extraction is obtained, medicinal extract and aqueous two-phase extraction liquid are pressed According to the volume ratio of 1:1 .25, obtains the upper phase of extraction and extract lower phase, double-aqueous phase system used in aqueous two-phase extraction is by poly- second two Alcohol, sodium chloride and water are constituted, and mass percent of the polyethylene glycol in the double-aqueous phase system is 15%, and sodium chloride is described double Mass percent in aqueous phase system is 15%, and surplus is water, and the weight average molecular weight of the polyethylene glycol is 8000；

S64, the aqueous phase extracted for obtaining the step S63, use molecular cut off for the ultrafiltration membrane desalination of 1000-100000, It obtains.