CN1089211C - Developing method of self-incompatible tine and homonuclear maintainer line of cabbage-type rape - Google Patents
Developing method of self-incompatible tine and homonuclear maintainer line of cabbage-type rape Download PDFInfo
- Publication number
- CN1089211C CN1089211C CN98121741A CN98121741A CN1089211C CN 1089211 C CN1089211 C CN 1089211C CN 98121741 A CN98121741 A CN 98121741A CN 98121741 A CN98121741 A CN 98121741A CN 1089211 C CN1089211 C CN 1089211C
- Authority
- CN
- China
- Prior art keywords
- self
- line
- selfing
- incompatible
- generation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention belongs to the improvement of a selecting and breeding method of the self-incompatible maintenance lines of cabbage type rape. In the improvement of the selecting and breeding method, by utilizing a selected and bred cabbage type rape self-incompatible line 271, a self-incompatible line evolved from the cabbage type rape self-incompatible line 271, and a self-incompatible maintenance line in the cabbage type rape and with completely different cell nuclear genotypes, a traditional method is innovated, namely that the cabbage type rape self-incompatible lines and homonuclear maintenance lines thereof are selected and bred. In the present invention, the difficulty of the mass multiplication of the cabbage type rape self-incompatible lines is overcome; cabbage type rape self-incompatible line three-way cross hybrids are converted into single cross hybrids, which is favorable to improving a heterosis rate.
Description
Technical field
The invention belongs to the improvement of brassica napus self-incompatible maintainer selection, be specifically related to the method for a kind of cabbage type rape of self incompatible lin and the seed selection of same nuclear maintenance line thereof.Its international Patent classificating number is A01H1/02.
Background technology
Studies have shown that in a large number that both at home and abroad the cabbage type rape variety species hybrid exists tangible hybrid vigour.The hybrid vigour of Wild cabbage type spring rape intervarietal hybrid can reach 10-20%, and winter rape variety species hybrid advantage can reach 20-40%.At present, utilize the approach of rape heterosis that the not affine and chemical emasculation of cytoplasmic male sterility, nuclear male sterility, selfing is arranged, wherein selfing is not affine is one of important channel.Forefathers have proposed many patterns of utilizing self incompatible line to produce crossbreed, comprise single cross hybrid, triple hybrid, double cross hybrid etc.In all these patterns, all fundamentally do not solve the prolific problem of self incompatible line.Fu Ting proposition cabbage type rape in 1981 selfing not affine three is (self incompatible line, self-incompatible maintainer and recovery system) hybrid production model, utilizes self-incompatible maintainer to breed self incompatible line in a large number.In this pattern, because maintenance line is different fully with the cell nucleus gene type of self incompatible line, the hybrid of being produced still is triple hybrid.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, provide a kind of easy, effective cabbage type rape selfing not affine with nuclear maintenance line selection.
The present invention can be achieved through the following technical solutions:
A kind of brassica napus self-incompatible maintainer selection, comprise that the cabbage type rape of self incompatible lin 271 that utilizes seed selection reaches by its self incompatible line that develops out and three series mating method thereof, wherein the seed selection of self incompatible line is to select for use 271 to reach by its other self incompatible line that develops out and the material hybridization that has the breeding objective proterties, in its filial generation, isolate the self incompatible line that meets breeding objective, the breeding of self incompatible line is adopted and is dialled flower bud selfing or the selfing of spray salt solution, self-incompatible maintainer and the seed selection that recovers to be adopt the test cross method to screen in cabbage type rape, self-incompatible maintainer is bred the employing selfing with recovery system or is interior brother and sister's friendship, described method is to adopt selfing not affine three to be, carries out self incompatible line and nuclear maintenance line seed selection together thereof, breeding and hybrid produce.
Method of the present invention comprises, utilizes selfing not affine three to be, the seed selection selfing is not affine with the nuclear maintenance line, breeds self incompatible line in a large number, produces interlinear hybrid;
Comprise that also described selfing is not affine to be made up of the concrete steps of following examples with the selection of nuclear maintenance line.
Advantage of the present invention:
1. a large amount of breeding problems of self incompatible line seed have been solved.
2. will pass by selfing not affine three is that triple hybrid is changed for selfing not affine three is a single cross hybrid, helps improving rape
Heterosis rate.
Embodiment
Embodiment 1:(selfing is not affine with the selection of examining maintenance line):
Selfing is not affine adopts following steps with the seed selection of nuclear maintenance line:
1. self incompatible line that meets target call and the hybridization of the not affine non-maintenance line of nuclear together of existing selfing that obtains with seed selection obtains the first generation of hybrid (F1);
2. F1 is dialled the flower bud selfing and obtain F2 generation;
3. the self incompatible line that meets target call and the F2 that continue to obtain with seed selection show self-compatible individual plant hybridization in generation, obtain first backcross generation (BC1);
4. BC1 is dialled the flower bud selfing and obtain BC1F2 generation;
5. the self incompatible line that meets target call and the self-compatible individual plant of the performance among the BC1F2 that continue to obtain with seed selection are hybridized, and obtain second backcross generation (BC2);
6. BC2 is dialled the flower bud selfing and obtain BC2F2 generation;
7. the self incompatible line that meets target call and the self-compatible individual plant of the performance among the BC2F2 that continue to obtain with seed selection are hybridized, and obtain third backcross generation (BC3);
8. at BC3 and in the generation later on, the method according to 6,7 repeats, and selects the self-compatible individual plant selfing of performance in generation at BC3F2 to BCnF2, selects the not affine maintenance line of examining together of selfing.
Embodiment 2 (propagation method that selfing not affine three is):
The breeding of self incompatible line is that self incompatible line and its are seeded under the condition that artificial isolation or the natural cover for defense isolate with the nuclear maintenance line is alternate, behind the seed maturity on self incompatible line the self incompatible line seed of results breeding.
Selfing is not affine with the breeding of nuclear maintenance line is to be seeded under the condition that artificial isolation or the natural cover for defense isolate with the nuclear maintenance line selfing is not affine, and the selfing of results breeding is not affine with nuclear maintenance line seed behind the seed maturity.
Selfing not the breeding of congenial restoring line be with selfing not congenial restoring line be seeded under the condition that artificial isolation or the natural cover for defense isolate the selfing of results breeding congenial restoring line seed not behind the seed maturity.
Selfing not affine three is that single cross hybrid seed production method is that congenial restoring line is not alternate is seeded under the condition that artificial isolation or the natural cover for defense isolate with self incompatible line and selfing, and the selfing not affine three that results are produced on self incompatible line behind the seed maturity is the single cross hybrid seed.
Claims (5)
1. brassica napus self-incompatible maintainer selection, comprise that the cabbage type rape of self incompatible lin 271 that utilizes seed selection reaches by its other self incompatible line that develops out and three series mating method thereof, wherein the seed selection of self incompatible line is to select for use 271 to reach by its other self incompatible line that develops out and the material hybridization that has the breeding objective proterties, in its filial generation, isolate the self incompatible line that meets breeding objective, the breeding of self incompatible line is adopted and is dialled flower bud selfing or the selfing of spray salt solution, self-incompatible maintainer and the seed selection that recovers to be adopt the test cross method to screen in cabbage type rape, self-incompatible maintainer is bred the employing selfing with recovery system or is interior brother and sister's friendship, it is characterized in that, described method is to adopt selfing not affine three to be, carries out self incompatible line and nuclear maintenance line seed selection together thereof, breeding and hybrid produce.
2. according to the described method of claim 1, it is characterized in that, utilize selfing not affine three to be, seed selection selfing discord is bred self incompatible line in a large number with the nuclear maintenance line, produces interlinear hybrid.
3. according to claim 1 or 2 described methods, it is characterized in that described selfing is not affine to be comprised with the selection of examining maintenance line; A. self incompatible line that meets target call and the existing selfing that obtains with seed selection is not affine
Non-with the hybridization of nuclear maintenance line, obtain the first generation of hybrid (F1); B. F1 is dialled the flower bud selfing and obtain F2 generation; C. the self incompatible line that meets target call and the F2 that continue to obtain with seed selection showed certainly in generation
Hand over affine individual plant hybridization, obtain first backcross generation (BC1); D. BC1 is dialled the flower bud selfing and obtain BC1F2 generation; E. continue the self incompatible line that meets target call that obtains with seed selection and the table among the BC1F2
Existing self-compatible individual plant hybridization obtains second backcross generation (BC2); F. BC2 is dialled the flower bud selfing and obtain BC2F2 generation; G. continue the self incompatible line that meets target call that obtains with seed selection and the table among the BC2F2
Existing self-compatible individual plant hybridization obtains third backcross generation (BC3); E. BC3 and after from generation to generation in, repeat according to the method for f, g, in BC3F2 to BCnF2 self-compatible individual plant selfing of choosing performance in generation, it is not affine with the nuclear maintenance line to select selfing.
4. according to the described method of claim 2, it is characterized in that, self incompatible line and its be seeded under the condition that artificial isolation or the natural cover for defense isolate with the nuclear maintenance line is alternate, behind the seed maturity on self incompatible line the self incompatible line seed of results breeding.
5. according to the described method of claim 2, it is characterized in that, congenial restoring line is not alternate is seeded under the condition that artificial isolation or the natural cover for defense isolate with self incompatible line and selfing, and the selfing not affine three that results are produced on self incompatible line behind the seed maturity is the single cross hybrid seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98121741A CN1089211C (en) | 1998-12-29 | 1998-12-29 | Developing method of self-incompatible tine and homonuclear maintainer line of cabbage-type rape |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98121741A CN1089211C (en) | 1998-12-29 | 1998-12-29 | Developing method of self-incompatible tine and homonuclear maintainer line of cabbage-type rape |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1258442A CN1258442A (en) | 2000-07-05 |
| CN1089211C true CN1089211C (en) | 2002-08-21 |
Family
ID=5227293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98121741A Expired - Fee Related CN1089211C (en) | 1998-12-29 | 1998-12-29 | Developing method of self-incompatible tine and homonuclear maintainer line of cabbage-type rape |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1089211C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010072048A1 (en) * | 2008-12-23 | 2010-07-01 | 华中农业大学 | Breeding method for brassica napus l. self-incompatible two-line hybrid |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810135B (en) * | 2010-03-19 | 2011-11-30 | 西北农林科技大学 | Seed production method for producing high-purity hybrid rape seeds by using double-pollination control system |
| CN104871803A (en) * | 2015-06-18 | 2015-09-02 | 云南农业大学 | Method for improving seeding performance of erigeron breviscapus |
| CN109006464B (en) * | 2018-08-15 | 2021-12-14 | 华中农业大学 | Simplified production method of rape male sterility hybrid F1 seed |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091582A (en) * | 1993-02-23 | 1994-09-07 | 陕西省农垦科技教育中心 | Sulphur rape three-way crossbreeding technology in the Wild cabbage type low mustard |
| CN1094885A (en) * | 1994-02-21 | 1994-11-16 | 江苏省农业科学院 | Cabbage type rape petalless germ plasm selection and breeding technique |
| CN1104418A (en) * | 1994-02-21 | 1995-07-05 | 江苏省农业科学院 | Breeding method for capitata double hybrid rape |
-
1998
- 1998-12-29 CN CN98121741A patent/CN1089211C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091582A (en) * | 1993-02-23 | 1994-09-07 | 陕西省农垦科技教育中心 | Sulphur rape three-way crossbreeding technology in the Wild cabbage type low mustard |
| CN1094885A (en) * | 1994-02-21 | 1994-11-16 | 江苏省农业科学院 | Cabbage type rape petalless germ plasm selection and breeding technique |
| CN1104418A (en) * | 1994-02-21 | 1995-07-05 | 江苏省农业科学院 | Breeding method for capitata double hybrid rape |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010072048A1 (en) * | 2008-12-23 | 2010-07-01 | 华中农业大学 | Breeding method for brassica napus l. self-incompatible two-line hybrid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1258442A (en) | 2000-07-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Luijten et al. | Demographic consequences of inbreeding and outbreeding in Arnica montana: a field experiment | |
| CN106069720A (en) | Brassica campestris L dihaploid induction system's selection-breeding cabbage type rape variety and the method for material | |
| CN111771716B (en) | Crop genetic breeding method for efficiently utilizing heterosis | |
| CN109997683B (en) | Rice double haploid breeding method based on haploid induction line | |
| Rotili et al. | Some aspects of breeding methodology in alfalfa | |
| CN106069718A (en) | The method of Brassica campestris L dihaploid induction system selection-breeding cabbage type rape cytoplasmic male sterile line | |
| CN1089211C (en) | Developing method of self-incompatible tine and homonuclear maintainer line of cabbage-type rape | |
| CN109006464B (en) | Simplified production method of rape male sterility hybrid F1 seed | |
| Chowdhury et al. | Natural outcrossing in grasspea | |
| Lee et al. | Intraspecific gametosomatic hybridisation in Petunia hybrida | |
| CN1843091A (en) | Rape cytoplasm male sterility three line selection and breeding method | |
| CN1127893C (en) | Two-line male sterile method for assortively breeding ecological-type cytoplasm of rape | |
| CN106035068B (en) | The method of rape dihaploid induction system selection and breeding mustard type rape kind and material | |
| CN101037672A (en) | Breeding method of plant cytoplasmic male sterility system | |
| CN108935079B (en) | Simplified seed production method of rape hybrid F1 seed | |
| CN111084095A (en) | Efficient breeding method adopting dwarf male-sterile wheat | |
| CN1307866C (en) | Rape cytoplasmic male sterility + self incompatibility hybrid superiority using method | |
| CN1442038A (en) | Mechanization seed making method of hybridized rice using three line method | |
| CN106605593B (en) | A kind of selection of the disease-resistant rape germplasm of floorboard with high oil content | |
| CN1052589A (en) | The Wild cabbage type rape kernet malesterile three-line seed technology | |
| CN109076954B (en) | Method for promoting earlier flowering of BT type japonica rice sterile line | |
| CN101107915B (en) | Orange yellow flower homozygous two-type line of dominant genic male sterility in brassica napus L. and application thereof | |
| CN111328706B (en) | Method for improving purity of complete sterile line and hybrid of brassica napus recessive epistatic interaction genic sterility | |
| CN106069721A (en) | Brassica campestris L dihaploid induction system's selection-breeding turnip type rape kind and the method for material | |
| CN112616651A (en) | Breeding method of glyphosate-resistant cotton genic male sterile line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |