CN108918467B - High-flux dry-type chemical detection device - Google Patents
High-flux dry-type chemical detection device Download PDFInfo
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- CN108918467B CN108918467B CN201810457098.1A CN201810457098A CN108918467B CN 108918467 B CN108918467 B CN 108918467B CN 201810457098 A CN201810457098 A CN 201810457098A CN 108918467 B CN108918467 B CN 108918467B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4738—Diffuse reflection, e.g. also for testing fluids, fibrous materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N2021/4735—Solid samples, e.g. paper, glass
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0438—Linear motion, sequential
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Abstract
The invention discloses a high-flux dry-type chemical detection device, which is provided with a base, a sample adding seat, a transmission mechanism, a temperature control incubation module and an optical detection module; the base is of a concave structure, a lower groove which is through from front to back is formed in the center of the base, a transmission mechanism is arranged in the lower groove and used for automatically pushing the reagent sheets, a track cover is arranged on the base, a track of the track cover corresponds to the lower groove in parallel, a push block is clamped on the track and used for manually pushing the reagent sheets, and a sample adding seat, a temperature control incubation module and an optical detection module are sequentially arranged at one end of the track; the detection device that the present case relates to can be used to carry out quick quantitative determination to the biochemical index in clinical blood sample, for example glucose, creatinine, glutamic-pyruvic transaminase, uremic toxin etc. moreover the device simple structure, detection efficiency is high, and stability is good, and convenient to use.
Description
Technical Field
The invention relates to the technical field of chemical detection devices, in particular to a high-throughput dry-type chemical detection device.
Background
In clinical test analysis, the results of a plurality of clinical test items can be rapidly obtained by using blood, urine or other body fluids to perform clinical analysis, and the method is one of the most accurate and direct methods for doctors to perform diagnosis. Among clinical laboratory analyses, wet chemical analysis, which is currently the most commonly used, uses a method called solution chemistry. Generally, instruments using wet chemical analysis are based on the colorimetric method of lambert-beer law. This detection method requires the following steps: blood drawing, centrifugation, reagent solution preparation and photoelectric detection. The disadvantages of such detection methods are: the reagent needs to be stored in a liquid mode, preparation needs to be carried out during detection, whole blood needs to be centrifuged to obtain serum, and the whole detection needs a long time.
Dry chemistry is also called solid phase chemistry, a multilayer film solid phase reagent technology is adopted, a liquid sample is directly added into a reagent carrier solidified into a special structure, namely a dry type reagent, water in the sample is used as a solvent, the reagent solidified on the carrier is dissolved and then is subjected to chemical reaction with a component to be measured in the sample, so that analysis and determination are carried out, and the concentration of the substance to be measured can be calculated according to the Kubelka-Munk theory.
Dry chemical analysis is a method in which a liquid test sample is directly applied to a commercially available dry reagent strip specifically manufactured for a different project, and moisture of the test sample is used as a solvent to cause a specific chemical reaction, thereby performing chemical analysis, as compared to wet chemical analysis. The method adopts a reflection photometry or differential electrode method as a measuring means, and mainly has the following characteristics: the accuracy is high, the speed is fast, and the inspection result can be made within 3-4min generally; the operation is simple and convenient, and daily correction is not needed; no other reagent needs to be stored or any solution needs to be prepared; the specimen does not need to be pretreated, and the multilayer film has the function of selective filtration, so that the influence of interference substances in the determination process is reduced; the sample is used in a small amount, and the water content in the sample is supplied during the reaction, thereby improving the measurement sensitivity. However, the multi-channel high-throughput dry-type chemical detection device in the prior art has a complex manufacturing process and high cost, and is not suitable for mass production, so a new high-throughput dry-type chemical detection device is needed to solve the above problems.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a high-throughput dry-type chemical detection device which can be used for quickly and quantitatively detecting biochemical indexes in clinical blood samples, such as glucose, creatinine, glutamic-pyruvic transaminase, uremic toxin, total bilirubin, triglyceride, lactic acid, ammonia, total cholesterol, uric acid, albumin, total protein and the like.
The invention provides a high-flux dry-type chemical detection device, which is provided with a base, a sample adding seat, a transmission mechanism, a temperature control incubation module and an optical detection module;
the base is of a concave structure, and a lower groove which is communicated with the front and the back is formed in the center of the base; a transmission mechanism is arranged in the lower groove and used for automatically pushing the reagent sheets; the base is provided with a track cover, and a track of the track cover corresponds to the lower groove in parallel; a pushing block is clamped on the track and used for manually pushing the reagent sheet; one end of the track is sequentially provided with a sample adding seat, a temperature control incubation module and an optical detection module;
the transmission mechanism comprises a motor, a gear, an upper rack and a lower rack; the tooth directions of the upper rack and the lower rack are opposite and are respectively coupled with the gear; the upper rack is connected with a push rod, and the push rod rotates along the positioning pin and is used for pushing the reagent sheet to advance.
Preferably, the sample adding seat frame is arranged above the track and used for fixing the liquid transferring gun so as to drip sample liquid to the reagent sheet.
Preferably, the temperature-controlled incubation module comprises an incubation bin and a spring block; the incubation bin is arranged in the lower groove and is used for accommodating the reagent sheet; the spring block is located and incubates storehouse top for push reagent piece down and incubate in the storehouse and to incubate the storehouse and keep warm thermal-insulated.
Preferably, the two sides of the incubation chamber are provided with Peltier patches to maintain the temperature of the incubation chamber at 37 +/-1 ℃; the incubation bin can accommodate 5 reagent sheets at most simultaneously.
Preferably, the lens of the optical detection module is opened at the bottom of the lower groove, and a light shielding cover is arranged above the lens for shielding stray light.
Preferably, one side of the reagent sheet is provided with a groove, and the groove is matched with a push rod connected with the upper rack, so that the push rod is clamped in the groove to further push the reagent sheet to advance.
Preferably, the base is provided with a garbage box outside the side provided with the optical detection module for collecting the detected reagent sheet.
Preferably, the operation of the detection device is as follows:
1) placing the reagent sheet in the track, pushing the push block, and manually pushing the reagent sheet to the lower part of the sample adding seat;
2) placing a liquid transfer gun on the sample adding seat, and dripping sample liquid onto the reagent sheet through the liquid transfer gun;
3) starting a motor in the transmission mechanism, enabling the motor to rotate forwards, enabling the upper rack to move forwards, enabling the lower rack to move backwards, and enabling the upper rack and the push rod to push the reagent piece to the temperature control incubation module from the lower part of the sample adding seat;
4) after the incubation of the reagent sheet is finished, the motor rotates reversely, the upper rack retreats, the lower rack advances, and the lower rack pushes the reagent sheet to the optical detection module for optical detection;
5) after the reagent plate is detected, the motor rotates reversely, and the lower rack pushes the reagent plate to a discarding position; the motor rotates forwards, the upper rack and the lower rack return to the initial positions, and the same operation is repeated for the next reagent sheet.
The invention has the beneficial effects that: the dry chemical detection device provided by the invention can be used for rapidly and quantitatively detecting biochemical indexes in clinical blood samples, and has the advantages of simple structure, high detection efficiency, good stability and convenience in use; through the cooperation of all parts of the device, 5 reagent sheets can be incubated at most simultaneously, so that a plurality of reagent sheets react simultaneously, and no waiting for dripping sample liquid is needed, thereby realizing high-flux detection and effectively improving the working efficiency and convenience; according to the invention, the optical detection module is arranged at the end of one end of the lower groove of the base, and the lower rack is driven by the gear to rotate reversely to automatically push the reagent sheet at the lowest part of the incubation bin from the incubation bin to the lens aligned with the optical detector for optical detection; after detection, the reagent sheet is directly pushed out of the lower groove by the lower rack, is separated from the base and enters the garbage box, and pollution-free recovery is realized.
Drawings
FIG. 1 is a schematic structural diagram of a high-throughput chemical detection apparatus;
FIG. 2 is a front view of a cross-sectional structure of the high-throughput chemical detection apparatus;
the device comprises a base 1, a sample adding seat 2, a transmission mechanism 3, a temperature control incubation module 4, an optical detection module 5, a garbage box 6, a lower groove 11, a rail cover 12, a rail 13, a push block 14, a reagent sheet 15, a gear 31, an upper rack 32, a lower rack 33, a push rod 34, an incubation bin 41, a spring block 42, a lens 51 and an optical detector 52.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The invention provides a high-flux dry-type chemical detection device, which mainly comprises a base 1, a sample adding seat 2, a transmission mechanism 3, a temperature control incubation module 4 and an optical detection module 5, as shown in a figure 1-2; the reagent bottle comprises a base 1, a transmission mechanism 3, a track cover 12, a pushing block 14 and a reagent tablet 15, wherein the base 1 is of a concave structure, a lower groove 11 which is through from front to back is formed in the center of the base, the transmission mechanism 3 is arranged in the lower groove 11 and used for automatically pushing the reagent tablet 15, the track cover 12 is buckled on the base 1, a track 13 of the track cover 12 corresponds to the lower groove 11 in parallel, the length of the track cover is shorter than that of the lower groove 11, and the pushing block 14 is clamped on the track 13; the part of the lower groove 11, which is longer than the rail 13, is sequentially provided with a temperature control incubation module 4 and an optical detection module 5, the sample adding seat 2 is erected above the tail end of the rail 13 and used for fixing a liquid transferring gun to dropwise add sample liquid to the reagent sheet 15, and the temperature control incubation module 4 and the optical detection module are arranged in the lower groove 11 which is longer than the rail 13.
Furthermore, the transmission mechanism 3 comprises a motor, a gear 31, an upper rack 32 and a lower rack 33, wherein the upper rack 32 and the lower rack 33 have opposite tooth directions and are respectively coupled with the gear 31, and a push rod 34 is connected to the upper rack 32, and the push rod 34 rotates along the positioning pin and is used for assisting in pushing the reagent sheet 15 to advance; when the motor rotates forwards, the upper rack 32 moves forwards, the lower rack 33 moves backwards, and the upper rack 32 pushes the reagent sheet 15 from the sample adding position to the incubation position; when the motor rotates reversely, the upper rack 32 retreats, the lower rack 33 advances, and the lower rack 32 pushes the reagent sheet 15 from the incubation position to the detection position and then from the detection position to the discarding position; when the upper rack 32 moves forward, the push rod 34 can prop against the groove of the reagent plate 15 to push forward, and when the upper rack 32 moves back into the track cover 12, the push rod 34 can rotate to the lower part to avoid interference; in the scheme, one side of the reagent sheet 15 is provided with a groove, and the groove is matched with a push rod 34 connected with an upper rack 32, so that the push rod is clamped in the groove to further push the reagent sheet 15 to move forward stably.
Furthermore, the temperature control incubation module 4 comprises an incubation bin 41 and a spring block 42, the incubation bin 41 is arranged in the lower groove and used for accommodating the reagent sheets, 5 reagent sheets 15 are accommodated at most simultaneously, the spring block 42 is located above the incubation bin 41 and can apply a downward force to the reagent sheets 15 to prevent the reagent sheets from being jacked up by the rack, and the heat insulation effect is achieved, and peltier patches are installed on two sides of the incubation bin 41 to maintain the temperature of the incubation bin at 37 +/-1 ℃.
Furthermore, the lens 51 of the optical detection module 5 is opened at the bottom of the lower groove 11, and a light shielding cover is arranged above the lens 51 for shielding stray light and avoiding stray light interference; a garbage box 6 is arranged outside one side of the base 1 provided with the optical detection module 5 and used for collecting the detected reagent sheet 15.
The working process of the detection device is as follows: a user places the reagent sheet on the track, manually operates the push block, and pushes the reagent sheet to the sample adding position; the liquid transferring gun is arranged on the sample adding seat, and the sample is dripped into the reagent sheet; the instrument starts to operate, the motor rotates forwards, the upper rack pushes the reagent sheets to the incubation position from the sample adding position, and the incubation bin can incubate 5 reagent sheets at most simultaneously; after incubation for a certain time, the motor rotates reversely, the lower rack pushes the reagent sheet at the lowest part of the incubation bin to a detection position, and optical detection is carried out; after the detection is finished, the motor rotates reversely, the lower rack pushes the reagent sheet to a discarding position, and the reagent sheet falls into a garbage box; the motor rotates forwards, and the rack returns to the initial position; the same procedure is performed for the next reagent piece.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (6)
1. A high-flux dry-type chemical detection device is characterized by comprising a base, a sample adding seat, a transmission mechanism, a temperature control incubation module and an optical detection module;
the base is of a concave structure, and a lower groove which is communicated with the front and the back is formed in the center of the base; a transmission mechanism is arranged in the lower groove and used for automatically pushing the reagent sheets; the base is provided with a track cover, and a track of the track cover corresponds to the lower groove in parallel; a pushing block is clamped on the track and used for manually pushing the reagent sheet; one end of the track is sequentially provided with a sample adding seat, a temperature control incubation module and an optical detection module;
the transmission mechanism comprises a motor, a gear, an upper rack and a lower rack; the tooth directions of the upper rack and the lower rack are opposite and are respectively coupled with the gear; the upper rack is connected with a push rod, and the push rod rotates along the positioning pin and is used for pushing the reagent sheet to advance;
the lens of the optical detection module is arranged at the bottom of the lower groove, and a shading cover is arranged above the lens and used for shading stray light;
the operation process of the detection device is as follows:
1) placing the reagent sheet in the track, pushing the push block, and manually pushing the reagent sheet to the lower part of the sample adding seat;
2) placing a liquid transfer gun on the sample adding seat, and dripping sample liquid onto the reagent sheet through the liquid transfer gun;
3) starting a motor in the transmission mechanism, enabling the motor to rotate forwards, enabling the upper rack to move forwards, enabling the lower rack to move backwards, and enabling the upper rack and the push rod to push the reagent piece to the temperature control incubation module from the lower part of the sample adding seat;
4) after the incubation of the reagent sheet is finished, the motor rotates reversely, the upper rack retreats, the lower rack advances, and the lower rack pushes the reagent sheet to the optical detection module for optical detection;
5) after the reagent plate is detected, the motor rotates reversely, and the lower rack pushes the reagent plate to a discarding position; the motor rotates forwards, the upper rack and the lower rack return to the initial positions, and the same operation is repeated for the next reagent sheet.
2. The testing device of claim 1, wherein the sample loading frame is disposed above the rail for holding a pipette for applying sample liquid to the reagent pad.
3. The detection device according to claim 1, wherein the temperature-controlled incubation module comprises an incubation bin and a spring block; the incubation bin is arranged in the lower groove and is used for accommodating the reagent sheet; the spring block is located and incubates storehouse top for push reagent piece down and incubate in the storehouse and to incubate the storehouse and keep warm thermal-insulated.
4. The detection device according to claim 3, wherein Peltier is installed on two sides of the incubation chamber to maintain the temperature of the incubation chamber at 37 ± 1 ℃; the incubation bin can accommodate 5 reagent sheets at most simultaneously.
5. The detecting device for detecting the rotation of the motor rotor according to the claim 1, wherein a groove is formed in one side of the reagent plate, and the groove is matched with a push rod connected with the upper rack, so that the push rod is clamped in the groove to further push the reagent plate to advance.
6. The detecting device according to claim 1, wherein the base is provided with a waste box for collecting the detected reagent sheet at an outside of a side where the optical detecting module is provided.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810457098.1A CN108918467B (en) | 2018-05-14 | 2018-05-14 | High-flux dry-type chemical detection device |
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| CN201810457098.1A CN108918467B (en) | 2018-05-14 | 2018-05-14 | High-flux dry-type chemical detection device |
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| CN108918467B true CN108918467B (en) | 2021-05-18 |
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| CN112705282B (en) * | 2019-10-25 | 2023-05-02 | 深圳华大智造科技股份有限公司 | Combined platform and detection device applying same |
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