CN108904556A - A kind of Thesium chinese extract powder is used to promote the purposes of children's skeleton development - Google Patents

Abstract

The present invention provides the purposes that a kind of Thesium chinese extract powder is used to promote children's skeleton development, which is characterized in that in preparation for promoting the application in children's skeleton development drug.

Description

A kind of Thesium chinese extract powder is used to promote the purposes of children's skeleton development

Technical field

The present invention relates to tcm development technical field, in particular to a kind of Thesium chinese extract powder promotes bone in children in preparation Bone develops the application in drug.

Background technique

In the prior art, children's skeleton development problem is many parent's concerns, if various activities movement or The caused skeleton development problems such as life poor form and nutrient absorption are bad, mostly pass through health care product or physicsl correct Mode, such as：

Chinese patent：Patent No.：A kind of children of ZL201410439546.7 promote to increase nourishing unpounded rice volume and its preparation Method is mainly promoted children's skeleton development by brown rice, and provided simply by simple nutrition, can only be corrected light The problem of micro- depauperation, effect is unobvious.

Chinese patent：201210191353.5, the children for promoting children's health growth and development to increase encourage shoes and pass through physics Mode stimulates and promotes child development, but does not have much effects the problem of depauperation caused by bone, and pass through Magnetic force injures children's torso others to promote development to be easy to cause.

And the Thesium chinese extract powder main function provided in the prior art is bacteriostasis thesine II to golden yellow Portugal Grape coccus, micrococcus catarrhalis, typhoid bacillus, proteus, shigella dysenteriae have inhibiting effect；Succinic acid to Candida albicans without Inhibiting effect.Thesine I only has inhibiting effect to staphylococcus aureus, micrococcus catarrhalis, shigella dysenteriae.It can be with heat-clearing solution Poison, kidney tonifying arresting seminal emission.Acute mastitis is controlled, pneumonia, lung abscess, tonsillitis, the infection of the upper respiratory tract, lumbago due to the kidney deficiency is dizzy, loses Essence, spermatorrhoea.Not any experiment and open source literature, which have, discloses the Thesium chinese extract powder in the technology in terms of promoting skeleton density Hold.

Summary of the invention

The present invention provides a kind of Thesium chinese extract powder in preparation for promoting the application in children's skeleton development drug.

To achieve the goals above, the present invention provides following technical scheme：

Inventor is found surprisingly that Thesium chinese extract powder promotes the purposes in children's skeleton development drug, hundred stamens in preparation The various forms of medicaments such as aqua, plaster can be made in careless extract powder, by the way that Immature rat, (birth 21 days, is equivalent to 2 years old Children) tested to obtain data be able to verify that medicament made of Thesium chinese extract powder for Immature rat bone growth, especially It is that the growth of male rat bone has certain facilitation, is mainly shown as and promotes bone growth, skeleton development index shin bone Gross density and cortical density increase, and promote children's skeleton development to have clear and obvious action clinic.

Specific embodiment

For a better understanding of the technical solution of the present invention, embodiment provided by the invention is described below in detail.

Test sample：

Thesium chinese extract powder；Character：Yellowish-brown powder；Gas is micro-, sweet, slight bitter；Content：17.1mg/g is (with Kaempferol C15H10O6 meter)；Unit is provided：Nine Hua Huayuan pharmaceutcal corporation, Ltd of Anhui；Lot number： 170308；Validity period：2 months 2019； Specification：2.7kg/ bag；Preservation condition：Sealing, refrigeration；Preparation method：Required preparation is made with deionized water.

Deionized water；Unit is provided：Upper seascape Pure water preparation Technology Co., Ltd.；Lot number：DI20170510；Specification： 25L/ barrels；Preservation condition：Closed, room temperature.

Sodium chloride injection；Unit is provided：Hunan Cologne Pharmaceutical Co., Ltd；Lot number：K140203；Specification：500ml/ Bottle；Ingredient：0.9%NaCl；Preservation condition：It is room temperature, closed.

Pentobarbital sodium salt (sodium pentobarbital)；Unit is provided：Hefei gold innovative material is limited Company；Lot number：201301；Specification：25g/ bottles；Ingredient：Yellow Jackets；Content：>=99.03%；Use concentration：0.03g/ ml；Preservation condition：It is room temperature, closed；Preparation method：It is prepared with sodium chloride injection.

Predominantly detect instrument：

Computed tomographic scanner (pQCT, peripheral Quantitative Computed Tomography, XCT Research SA) Stratec company of Germany.

Experimental animal：

SD (health status is stablized) Immature rat, 120,21 days, connects by SPF grades (no-special pathogen grade experimental animal) 35.0~45.2g of time receiving is purchased from the western Poole-Bi Kai experimental animal in Shanghai Co., Ltd, Quality of Experimental Animals quality certification serial number： 0304660；Experimental animal production licence number：SCXK (Shanghai) 2013-0016, Shanghai City Science and Technology Commission issues；Experiment Animal uses credit number：SYXK (Shanghai) 2013-0027, Shanghai City Science and Technology Commission issues；

Feed, padding and drinking water：Feed is the breeding mouse material of Shanghai Shi Lin Biotechnology Co., Ltd production, lot number： 20170601, it is up-to-standard, SPF grades of animal houses are entered after high pressure sterilization.It is primary to inspect feed this center every year by random samples, commission Shanghai Forage Quality Supervision & Inspection Station's detection examines thick protein, thick rouge according to corresponding GB and GB/T (national standard) Fat, crude fibre, moisture, calcium, total phosphorus content and total number of bacteria, coliform, aflatoxin B1, arsenic, lead, cadmium and mercury Deng quality is qualified.Sawdust padding is provided by Baoshan District, Shanghai good fortune standing grain experimental article supply station, through autoclave sterilization；It drinks Water is high pressure sterilization Drinking Water, and every year, detection was primary, the detection of commission Xuhui District of Shanghai disease prevention and control center, Referring to standards for drinking water quality, detection turbidity, total plate count, free chlorine residual and total coli group etc., examined project is equal Meet the requirement of Appreciation gist.

Rearing conditions and environment：Animal is in Shanghai Family Planning Science and Research Institute.'s (Chinese growth difference drug toxicology inspection Measured center) the interior raising of SPF grades of animal houses, in 400 × 350 × 200mm3 plastics cage, every cage raises same sex animal 5 for raising, Free water is ingested, and 22.0~24.4 DEG C of room temperature, relative humidity 51.8%~66.2% is illumination 12 hours, 12 hours dark, Take a breath 12 times/h, all-fresh air.

Sample preparation methods：

After experiment, as needed, bone tissue (shin bone) separation in time, removal bone surrounding tissue are taken immediately, is fixed on It is to be determined in 70% ethyl alcohol.

Bone mineral density method：

It is downward by basic point of osteoepiphyseal line after showing the form of proximal tibia first to shin bone sample longitudinal scanning when detection Make tomography measurement at 3.0mm, reflect influence of the test-compound to bone tissue different parts structure,

With total bone density, trabecular bone density and cortical bone density, mg/cm3 is indicated；Data carry out rough estimates processing, Every group of result is indicated with mean+SD.Comprehensive descision is tested influence of the compound to experimental animal bone density.

Grouping and medication：

Group technology：If totally 4 groups of vehicle control group (deionized water), the low, medium and high dosage group of Thesium chinese extract powder, low, Neutralize high dose be respectively 0.6,9.0 and 15.0g medicinal extract/kg (be respectively equivalent to 11.0,165.0 and 274.8g crude drug amount/ kg)；Rat is grouped at random according to weight levels, and every group 30,It is fifty-fifty, amount to 120；

Medication：

Administration frequency：1 times/day；Administration route：Stomach-filling；Dosage：30ml/kg weight；The time limit is administered：4 weeks.

Experimental method and observation index：

Experimental method：

It is administered using young animal PND21 (after birth 21 days), at least observes the general status of 1 animal daily；

Selection meets the animal of requirement of experiment, 1 times/day of gastric infusion after grouping, and PND21 starts total administration 4 weeks；

It is administered after 4 weeks and to cut open the animal for killing 2/3 for 24 hours (D29), remaining animal is cutd open after restoring observation 2 weeks and kills；And it is given in last Skeleton specimen is acquired after medicine, measures tibia length and density, and carry out pathological examination；

(D43), which is cutd open, after remaining animal restores observation 2 weeks kills remaining 1/3 animal, and observation index is same as above.

Observation index：

Crown rump length：Administration D29 takes plan dissection animal, its crown rump length is measured after anesthesia；

Bone density：Administration D29 takes plan dissection animal, and female animals side shin bone is taken after anesthesia, measures length and bone is close Degree, separation in time, removal bone surrounding tissue, is fixed in 75% ethyl alcohol, detects test sample to bone tissue different parts structure It influences, is indicated with total bone density, trabecular bone density and cortical bone density.

Statistical analysis：

For statistical analysis using SPSS statistical software, the measurement datas such as weight and food ration are indicated with x ± SD, between group One-way analysis of variance or non-parametric test when comparing；The enumeration datas such as Growth and development index compliance rate and positive rate are with hundred Point rate indicates, chi-square criterion when comparison among groups, other enumeration datas describe its variation tendency.

As a result：

Influence to young age hero mouse skeletal system：

Shin bone is long：At the end of administration phase and convalescence, compared with vehicle control group, low, medium and high dosage group hero mouse shin bone Length is showed no statistical difference (P > 0.05)；

Bone density：At the end of the administration phase, compared with vehicle control group, middle and high dosage group gross density and cortical density increase (P < 0.05 or P < 0.01), remaining has no statistical difference (P > 0.05)；At the end of convalescence, each group is showed no statistics Difference (P > 0.05).

Influence to young age female mice skeletal system：

Shin bone is long：At the end of administration phase and convalescence, compared with vehicle control group, low, medium and high dosage group female mice shin bone Length is showed no statistical difference (P > 0.05)；

Bone density：At the end of the administration phase, compared with vehicle control group, female mice low dose group Trabecular density slightly reduces (P < 0.05), remaining has no statistical difference (P > 0.05)；At the end of convalescence, although having no statistical difference, female mice Increase trend (P > 0.05) is presented in low dose group tibial cortex density.

Analysis：

At the end of the administration phase, each group shin bone length has no statistical difference (P > 0.05)；Think to give Thesium chinese extract powder Will not have an adverse effect to male and female Immature rat bone growth；

At the end of the administration phase, compared with vehicle control group, each dosage group shin bone length of female mice, gross density and cortical density etc. Have no statistical difference (P > 0.05)；Middle and high dosage group hero mouse shin bone gross density and cortical density increase (P < 0.05 or P < 0.01), increase amplitude 2.7%~8.0%, it is believed that stomach-filling gives Thesium chinese extract powder 4 weeks, raw for Immature rat bone Long, especially male rat bone growth has certain facilitation, to further promote children's skeleton development.

Conclusion：

Stomach-filling in 4 weeks continuous to SD Immature rat (PND21) is given 0.6,9.0 and 15.0g medicinal extract/kg and (is respectively equivalent to 11.0,165.0 and 274.8g crude drug amount/kg) dosage Thesium chinese extract powder, using stomach-filling give the deionized water of equivalent as Vehicle controls.Stomach-filling gives Thesium chinese extract powder 4 weeks, and Immature rat bone growth, especially male rat bone are grown Certain facilitation is had, is mainly shown as and bone growth, skeleton development index shin bone gross density and cortical density is promoted to increase Add, skeleton development is promoted to clinic, healing and treatment osteoporosis after fracturing is promoted to have certain effect.

(the administration phase terminates for table 1, the influence to male mouse Growth and development index in the stomach-filling of Thesium chinese extract powder 4 weeks；x±SD)

Note：Compared with vehicle control group, * P < 0.05, * * P < 0.01.

(convalescence terminates for table 2, the influence to male mouse Growth and development index in the stomach-filling of Thesium chinese extract powder 4 weeks； x±SD)

It is provided for the embodiments of the invention the purposes that a kind of Thesium chinese extract powder is used to promote children's skeleton development above It is described in detail, for those of ordinary skill in the art, thought according to an embodiment of the present invention, in specific embodiment party There will be changes in formula and application range, in conclusion the contents of this specification are not to be construed as limiting the invention.

Claims (1)

1. the purposes that a kind of Thesium chinese extract powder is used to promote children's skeleton development, which is characterized in that in preparation for promoting Application in virgin skeleton development drug.