CN108902637A - One plant-derived bacteriostatic extractive - Google Patents
One plant-derived bacteriostatic extractive Download PDFInfo
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- CN108902637A CN108902637A CN201810289500.XA CN201810289500A CN108902637A CN 108902637 A CN108902637 A CN 108902637A CN 201810289500 A CN201810289500 A CN 201810289500A CN 108902637 A CN108902637 A CN 108902637A
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- 230000003385 bacteriostatic effect Effects 0.000 title claims abstract description 54
- 241000196324 Embryophyta Species 0.000 claims abstract description 56
- 238000000034 method Methods 0.000 claims abstract description 26
- 229930014669 anthocyanidin Natural products 0.000 claims abstract description 8
- 235000008758 anthocyanidins Nutrition 0.000 claims abstract description 8
- 244000223014 Syzygium aromaticum Species 0.000 claims abstract description 6
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims abstract description 6
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- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims abstract description 5
- 244000089698 Zanthoxylum simulans Species 0.000 claims abstract description 5
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- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims abstract description 5
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- 239000011347 resin Substances 0.000 claims description 25
- 229920005989 resin Polymers 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 22
- 238000002386 leaching Methods 0.000 claims description 20
- 238000010521 absorption reaction Methods 0.000 claims description 19
- 239000003513 alkali Substances 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 14
- 238000002137 ultrasound extraction Methods 0.000 claims description 14
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
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- 239000012528 membrane Substances 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 238000011084 recovery Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 4
- 239000000908 ammonium hydroxide Substances 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 150000002212 flavone derivatives Chemical class 0.000 claims description 3
- 239000005452 food preservative Substances 0.000 claims description 3
- 235000019249 food preservative Nutrition 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims 3
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- 239000000022 bacteriostatic agent Substances 0.000 description 6
- 239000005416 organic matter Substances 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
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- 241000894006 Bacteria Species 0.000 description 3
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
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- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 240000001549 Ipomoea eriocarpa Species 0.000 description 1
- 235000005146 Ipomoea eriocarpa Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 125000000254 aspartoyl group Chemical group 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The invention discloses a plant-derived bacteriostatic extractives, belong to plant biological extract technical field, the bacteriostatic extractive is with anthocyanidin and general flavone flavonoids as main component, the raw material of bacteriostatic extractive is tangerine peel, Chinese prickly ash, cloves, honeysuckle, wrinkled giant hyssop, compositae plant, using 15 ~ 20% anthocyanidin contents as the plant containing anthocyanin of standard dose, kuh-seng, garlic, one of cumin is a variety of, the purity is high of plant-derived bacteriostatic extractive of the invention, bacteriostatic activity is high, bacteriostasis category is wide, and extracting method simple possible, extraction efficiency is high, Objective extraction object yield and purity is high.
Description
Technical field
The invention belongs to plant biological extract technical fields, and in particular to a plant-derived bacteriostatic extractive.
Background technique
With the transformation of health of people idea, plant-based bacteriostat is antibacterial without chemistry because its is highly-safe, easy to use
The problems such as health hazard brought by agent and environmental pollution, and the advantages that holding conditions are easy to control, it is antibacterial to have become various countries
The direction of fresh-keeping research and hot spot.Therefore, it finds the plant-based bacteriostat nontoxic to biology and Environmental security and has become one
Kind trend.China's plant resources very abundant, holds out broad prospects in the development and application of food bacteriostatic agent.
Bacteriostatic agent is exactly the substance that can inhibit bacterial growth.Bacteriostatic agent possibly can not kill bacterium, but it can inhibit thin
The growth of bacterium, prevention bacteria breed is excessive, harm is healthy.All essential oils more or less have the ability for inhibiting bacterial growth.
Some essential oils only inhibit the growth of certain special germs, and some essential oils, inhibit the type of germ very much.Meanwhile as long as very
A small amount of essential oil, so that it may generate good fungistatic effect.
In recent years, there are many research report of natural bacteriostatic substance, and wherein the plant of good antimicrobial effect can be used as bateriostatics
The potential alternate source of matter, to be provided with the application value and economic value of research.Due on the market to Extracts from Plant Recourses
Extraction process more falls behind, and not comprehensive enough to the development and utilization of plant content, the yield and purity of extract are relatively low, therefore
Developing a kind of effective extracting method is very necessary to the research for promoting the antibacterial aspect of plant.
Summary of the invention
The purpose of the present invention is to provide a plant-derived bacteriostatic extractive, the purity is high of bacteriostatic extractive, bacteriostatic activities
Height, bacteriostasis category is wide, and extracting method simple possible, and extraction efficiency is high, to Objective extraction object yield and purity
It is high.
The technical solution that the present invention is taken to achieve the above object is：One plant-derived bacteriostatic extractive, this is antibacterial to mention
Taking object is with anthocyanidin and general flavone flavonoids as main component.
Preferably, the raw material of bacteriostatic extractive be tangerine peel, Chinese prickly ash, cloves, honeysuckle, wrinkled giant hyssop, compositae plant, with 15 ~
20% anthocyanidin content is one of the plant containing anthocyanin of standard dose, kuh-seng, garlic, cumin or a variety of, above-mentioned plant original
Bacteriostatic active ingredients content contained in material is high, is conducive to improve single-trial extraction amount, and plurality of raw materials compounding is extracted, extract
With synergy or summation action, make to prepare resulting bacteriostatic extractive not only bacteriostatic activity with higher and bacteriostasis type
Range is wide, and above-mentioned raw materials are easy to acquirement, affordable, can reduce manufacturing cost, is suitable for industrialized production.
Preferably, the extracting method of bacteriostatic extractive includes crushing, alcohol steep, purifying, drying, the specific steps are：
Crushing operation is：Plant material is taken, cleans and dries, 8 ~ 20min of crushing in pulverizer is put into, smashes it through 150 ~ 300 mesh
After raw material is carried out pulverization process the uniform powder of particle diameter distribution can be obtained, while increasing the specific surface area of powder in sieve,
During following process, reaction contact area is increased, to improve reaction speed, is not only saved process time, but also is improved
Processing efficiency；
Alcohol steep operates：Smashed plant material powder is taken, is 1 by solid-liquid ratio:8 ~ 16 add into plant material powder
Leaching liquor impregnates, 1.6 ~ 2.3h of ultrasonic extraction, filters, then pressing solid-liquid ratio is 1:5 ~ 10 add leaching liquor, ultrasonic extraction into filter residue
1.6 ~ 2.3h is filtered, and merges filtrate twice, and above-mentioned leaching liquor is 55 ~ 70% ethanol solutions, in ethanol solution containing 0.13 ~
0.22% aspargine, the weight ratio of D- aspargine and L- aspargine in aspargine are 100:0.55~
0.78, above-mentioned soaking temperature is 30 ~ 40 DEG C, the time be 16 ~ for 24 hours, ultrasonic extraction temperature is 55 ~ 65 DEG C, power is 160 ~ 230W,
The aspargine being added during alcohol steep can assist propagation of the ultrasonic wave in plant cell medium, utilize ultrasonic wave
Swirling effect, cavitation effect and the fuel factor that radiation pressure generates accelerate the diffusion of substance, the very big pressure that ultrasonic cavitation generates
Power is crushed plant cell wall by moment, causes turbulence effect, perturbation effect, interfacial effect and Mohaupt effect, makes mass transfer boundary
Layer is thinned, and the concentration gradient of solute is reduced in boundary layer, increases mass transfer rate, has dramatically speeded up extraction process, in addition, be added
Aspargine can also dissolubility of the reinforcing fiber cell surface impurity in ethanol solution, make cell surface that certain stream be presented
Dynamic state, so that fibrocyte surface gradually tends to smooth, pit is more equably exposed to cell surface, fiber bundle-like structure
Become loose, be conducive to intracellular organic matter release, significantly improve extraction rate, improves the extraction rate and yield of Objective extraction object, and
Make Objective extraction object bacteriostatic activity with higher；
Purification process is：Taking leaching liquor filmed passing tube is the ceramic membrane of 10 ~ 50nm, and the 0.08 ~ 0.15% of coating solution quality was added
Malonyl urea is 1 by solid-liquid ratio:Absorption resin is added in 12 ~ 20g/mL, and alkali cleaning, ethyl alcohol parses, after desorbed solution concentration and recovery ethyl alcohol
Decoloration, smart deresination are crossed respectively, it is spare, wherein absorption resin is one of S-8, D101,69M, HZ841, HPD100, P20
Or it is a variety of, alkali wash water is one of ammonium hydroxide and NaOH or a variety of, and alkali wash water dosage is 0.5 ~ 2BV, and flow velocity is 0.5 ~ 1.5BV/
H, macroporous absorbent resin are preferable to the selectivity of organic matter, not by shadow existing for inorganic salts and strong ion low molecular compound
It rings, the malonyl urea of addition is conducive to active constituent and forms more orderly arrangement in absorption resin surface, while can obviously increase
Add the hydrogen bond action and π-π action site in adsorption process, thus greatly improve absorption resin to the adsorption effect of active constituent,
Active constituent yield and purity in plant material are improved, the bacteriostatic activity of extract, the absorption selected in aforesaid operations are improved
Resin stable in physicochemical property, insoluble in acid, alkali and organic solvent, and absorption resin regeneration is convenient, parsing is mild, handling
It is good, bacteriostatic active ingredients extraction cost can be reduced；
Drying process is：Using oven drying, spray drying, revolution tank vacuum drying one of or it is a variety of.
Preferably, including anthocyanidin content in bacteriostatic extractive for 20 ~ 80wt%, general flavone content is 15 ~ 90wt%, this
Bacteriostatic active ingredients content is high in the bacteriostatic extractive of invention, good antimicrobial effect, before food preservative has biggish application
Scape.
Preferably, application of the plant-derived bacteriostatic extractive as food preservative.
Compared with prior art, beneficial effects of the present invention are:1）Bacteriostatic activity contained in plant material of the invention
Component content is high, is conducive to improve single-trial extraction amount, and plurality of raw materials compounding is extracted, and extract, which has synergy or is added, to be made
With making to prepare resulting bacteriostatic extractive not only bacteriostatic activity with higher and bacteriostasis category is wide；2）The present invention
The high in machining efficiency of extracting method of bacteriostatic extractive, yield it is high, extraction cost is low, and extracting method simple possible is suitable for
Industrialized production；3）The leaching step of the extracting method can dissolubility of the reinforcing fiber cell surface impurity in ethanol solution,
So that fiber bundle-like structure becomes loose, is conducive to intracellular organic matter release, significantly improves extraction rate, improve mentioning for Objective extraction object
Rate and yield are taken, and makes Objective extraction object bacteriostatic activity with higher；4）The purification process of the extracting method is conducive to live
Property ingredient in absorption resin surface form more orderly arrangement, while hydrogen bond action and π-in adsorption process can be obviously increased
π action site improves active constituent yield in plant material to greatly improve absorption resin to the adsorption effect of active constituent
And purity, improve the bacteriostatic activity of extract.
Specific embodiment
It is described in further detail with reference to embodiments：
Embodiment 1：
The extracting method of bacteriostatic extractive includes crushing, alcohol steep, purifying, drying, the specific steps are：
1）It crushes：Taking tangerine peel, Chinese prickly ash, cloves, honeysuckle, Taihang chrysanthemum, aubergine pigment is plant material, cleans and dries, is put into crushing
20min is crushed in machine, smashes it through 300 meshes, and after raw material is carried out pulverization process, the uniform powder of particle diameter distribution can be obtained,
The specific surface area for increasing powder simultaneously increases reaction contact area during following process, to improve reaction speed
Degree, is not only saved process time, but also improve processing efficiency；
2）Alcohol steep：Smashed plant material powder is taken, is 1 by solid-liquid ratio:16 add leaching liquor into plant material powder,
It impregnates, ultrasonic extraction 2.3h, filters, then pressing solid-liquid ratio is 1:10 into filter residue plus leaching liquor, ultrasonic extraction 2.3h are filtered, and closes
And filtrate twice, above-mentioned leaching liquor are 70% ethanol solution, in ethanol solution containing 0.22% aspargine, aspargine
In D- aspargine and L- aspargine weight ratio be 100:0.78, above-mentioned soaking temperature is 40 DEG C, the time is
For 24 hours, ultrasonic extraction temperature be 65 DEG C, power 230W, the aspargine being added during alcohol steep can assist ultrasound
Propagation of the wave in plant cell medium, using Ultrasonic Radiation pressure generate swirling effect, cavitation effect and fuel factor come
Accelerate the diffusion of substance, the enormous pressure that ultrasonic cavitation generates is crushed plant cell wall by moment, causes turbulence effect, perturbation
Mass transfer boundary layer is thinned in effect, interfacial effect and Mohaupt effect, and the concentration gradient of solute is reduced in boundary layer, increases mass transfer
Rate has dramatically speeded up extraction process, can also dissolubility of the reinforcing fiber cell surface impurity in ethanol solution, make cell table
Certain flow regime is presented in face, so that fibrocyte surface gradually tends to smooth, pit is more equably exposed to cell table
Face, fiber bundle-like structure become loose, are conducive to intracellular organic matter release, significantly improve extraction rate, improve mentioning for Objective extraction object
Rate and yield are taken, and makes Objective extraction object bacteriostatic activity with higher；
3）Purifying：Taking leaching liquor filmed passing tube is the ceramic membrane of 50nm, and 0.15% malonyl urea of coating solution quality was added, presses
Solid-liquid ratio is 1:Absorption resin, alkali cleaning is added in 20g/mL, and ethyl alcohol parses, and crosses decoloration, essence after desorbed solution concentration and recovery ethyl alcohol respectively
Deresination, spare, wherein absorption resin is S-8 resin, alkali wash water is NaOH solution, and alkali wash water dosage is 2BV, and flow velocity is
1.5BV/h, macroporous absorbent resin are preferable to the selectivity of organic matter, are not existed by inorganic salts and strong ion low molecular compound
Influence, the malonyl urea of addition is conducive to active constituent and forms more orderly arrangement in absorption resin surface, while can be bright
The aobvious hydrogen bond action and π-π action site increased in adsorption process, to greatly improve absorption of the absorption resin to active constituent
Effect improves active constituent yield and purity in plant material, improves the bacteriostatic activity of extract, select in aforesaid operations
Resin stable in physicochemical property is adsorbed, insoluble in acid, alkali and organic solvent, and absorption resin regeneration is convenient, parsing is mild, manipulation
Property is good, can reduce bacteriostatic active ingredients extraction cost；
4）It is dry：Using oven drying.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
It states.
Embodiment 2：
The extracting method of bacteriostatic extractive includes crushing, alcohol steep, purifying, drying, the specific steps are：
1）It crushes：Taking wrinkled giant hyssop, Chinese prickly ash, cloves, garlic, STEVIA REBAUDIANA, grape is plant material, cleans and dries, is put into pulverizer
11min is crushed, 160 meshes are smashed it through；
2）Alcohol steep：Smashed plant material powder is taken, is 1 by solid-liquid ratio:12 add leaching liquor into plant material powder,
It impregnates, ultrasonic extraction 2.1h, filters, then pressing solid-liquid ratio is 1:7 into filter residue plus leaching liquor, ultrasonic extraction 1.7h are filtered, and merges
Filtrate twice, above-mentioned leaching liquor are 60% ethanol solution, in ethanol solution containing 0.19% aspargine, in aspargine
D- aspargine and L- aspargine weight ratio be 100:0.59, above-mentioned soaking temperature is 35 DEG C, time 18h,
Ultrasonic extraction temperature is 60 DEG C, power 180W；
3）Purifying：Taking leaching liquor filmed passing tube is the ceramic membrane of 10 ~ 50nm, and 0.12% malonyl urea of coating solution quality was added,
It is 1 by solid-liquid ratio:16g/mL is added absorption resin, alkali cleaning, ethyl alcohol parsing, crossed after desorbed solution concentration and recovery ethyl alcohol respectively decoloration,
Smart deresination, spare, wherein absorption resin is D101 resin, alkali wash water is ammonium hydroxide, and alkali wash water dosage is 1.5BV, and flow velocity is
1.1BV/h；
4）It is dry：Using spray drying.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
It states.
Embodiment 3：
The extracting method of bacteriostatic extractive includes crushing, alcohol steep, purifying, drying, the specific steps are：It crushes：Take wrinkled giant hyssop,
Kuh-seng, cloves, garlic, STEVIA REBAUDIANA, morning glory are plant material, clean and dry, are put into pulverizer and crush 8min, smash it through
150 meshes；Alcohol steep：Smashed plant material powder is taken, is 1 by solid-liquid ratio:8 add extraction into plant material powder
Liquid impregnates, ultrasonic extraction 1.6h, filters, then pressing solid-liquid ratio is 1:5 into filter residue plus leaching liquor, ultrasonic extraction 1.6h are filtered,
Merge filtrate twice, above-mentioned leaching liquor is 55% ethanol solution, in ethanol solution containing 0.13% aspargine, aspartoyl
The weight ratio of D- aspargine and L- aspargine in ammonia is 100:0.55, above-mentioned soaking temperature is 30 DEG C, the time is
16h, ultrasonic extraction temperature is 55 DEG C, power 160W；Purifying：Taking leaching liquor filmed passing tube is the ceramic membrane of 10nm, and film was added
0.08% malonyl urea of solution quality is 1 by solid-liquid ratio:Absorption resin, alkali cleaning, ethyl alcohol parsing, desorbed solution is added in 12g/mL
Decoloration, smart deresination are crossed after concentration and recovery ethyl alcohol respectively, spare, wherein absorption resin is HPD100 resin, alkali wash water is ammonium hydroxide,
Alkali wash water dosage is 0.5BV, flow velocity 0.5BV/h；It is dry：Using revolution tank vacuum drying.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
It states.
Embodiment 4：
It is tested using the plant-derived bacteriostatic extractive that the present invention extracts：
It is test group that bacteriostatic extractive of the invention is drafted in test, and market general food bacteriostatic agent is control group, test material choosing
With fresh apple, pork and milk, apple is squeezed the juice before test, pork is twisted into muddy flesh.Cider, milk and muddy flesh are taken when test
Every kind of 10g, and be respectively put into the triangular flask of 3 sterilizings of test group, control group and blank group, each triangular flask is 50ml,
Sealing.The bacteriostatic extractive of the invention that addition 3ml concentration is 0.05% in 3 triangular flasks of test group, 3 three of control group
The food bacteriostatic agent that 3ml concentration is 0.05% is added in the bottle of angle, any extraneous ingredient is not added in 3 triangular flasks of blank group.It is all
Pasteurization again after sample is sealed is placed one month at normal temperature, and clump count is measured by sampling, and is converted into test group and right respectively
According to the bacteriostasis rate of group.
After sealed sample room temperature one month of pasteurization, respectively takes 10ml to carry out gradient dilution, take 10-1、10-2、
10-3Concentration be coated plate, cultivated at 37 DEG C for 24 hours, count clump count, according to formula calculate bacteriostasis rate, calculated result note
It records in table 1：
1 bacteriostasis rate calculated result of table
| Cider | Milk | Muddy flesh | |
| Test group | 95.7% | 96.1% | 96.3% |
| Control group | 84.2% | 68.3% | 70.5% |
By upper table data can test group it is ideal to the fungistatic effect of three kinds of food, and to the fungistatic effect of different food products
Differ smaller, and control group is not only affected to the fungistatic effect of food by different food products, and its whole fungistatic effect
It is poor, therefore can show that Extracts from Plant Recourses bacteriostatic activity of the invention is high and has preferable fungistatic effect, bacteriostasis range
Extensively, ideal to the fungistatic effect of different food products.
Routine techniques connection or the prior art of prior art dawn known to those skilled in the art in above-described embodiment,
Therefore it is not described in detail herein.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field
Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent
Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (10)
1. a plant-derived bacteriostatic extractive, it is characterised in that：The bacteriostatic extractive is based on anthocyanidin and general flavone
Want the flavonoids of ingredient.
2. a plant-derived bacteriostatic extractive according to claim 1, it is characterised in that：The original of the bacteriostatic extractive
Material be tangerine peel, Chinese prickly ash, cloves, honeysuckle, wrinkled giant hyssop, compositae plant, using 15 ~ 20% anthocyanidin contents be standard dose contain pattern
One of glycosides plant, kuh-seng, garlic, cumin are a variety of.
3. a plant-derived bacteriostatic extractive according to claim 1, it is characterised in that：The bacteriostatic extractive
Extracting method includes crushing, alcohol steep, purifying, drying, and the crushing operation is：Plant material is taken, cleans and dries, put
Enter 8 ~ 20min of crushing in pulverizer, smashes it through 150 ~ 300 meshes.
4. a plant-derived bacteriostatic extractive according to claim 3, it is characterised in that：The alcohol steep operation
For：Smashed plant material powder is taken, is 1 by solid-liquid ratio:8 ~ 16 into plant material powder plus leaching liquor, immersion are ultrasonic
1.6 ~ 2.3h is extracted, is filtered, then pressing solid-liquid ratio is 1:5 ~ 10 into filter residue plus leaching liquor, 1.6 ~ 2.3h of ultrasonic extraction are filtered, and closes
And filtrate twice.
5. a plant-derived bacteriostatic extractive according to claim 4, it is characterised in that：The leaching liquor is 55 ~ 70%
Ethanol solution contains 0.13 ~ 0.22% aspargine in the ethanol solution.
6. a plant-derived bacteriostatic extractive according to claim 4, it is characterised in that：The soaking temperature be 30 ~
40 DEG C, the time be 16 ~ for 24 hours, the ultrasonic extraction temperature is 55 ~ 65 DEG C, power is 160 ~ 230W.
7. a plant-derived bacteriostatic extractive according to claim 3, it is characterised in that：The purification process is：It takes
Leaching liquor filmed passing tube is the ceramic membrane of 10 ~ 50nm, 0.08 ~ 0.15% malonyl urea of coating solution quality is added, by solid-liquid ratio
It is 1:12 ~ 20g/mL, which is added, adsorbs resin, alkali cleaning, ethyl alcohol parsing, crosses decoloration respectively after desorbed solution concentration and recovery ethyl alcohol, essence takes off tree
Rouge, it is spare.
8. a plant-derived bacteriostatic extractive according to claim 7, it is characterised in that：The absorption resin be S-8,
One of D101,69M, HZ841, HPD100, P20 or a variety of, the alkali wash water are one of ammonium hydroxide and NaOH or more
Kind, alkali wash water dosage is 0.5 ~ 2BV, and flow velocity is 0.5 ~ 1.5BV/h.
9. a plant-derived bacteriostatic extractive according to claim 1, it is characterised in that：It is wrapped in the bacteriostatic extractive
It is 20 ~ 80wt% containing anthocyanidin content, general flavone content is 15 ~ 90wt%.
10. a plant-derived bacteriostatic extractive according to claim 1, it is characterised in that：The plant source is antibacterial to be mentioned
Take application of the object as food preservative.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110685148A (en) * | 2019-10-23 | 2020-01-14 | 成工业制衣（苏州）有限公司 | Novel antibacterial textile fabric |
| CN110840759A (en) * | 2019-10-15 | 2020-02-28 | 山东药品食品职业学院 | Anthocyanin bacteriostatic hand sanitizer and preparation method thereof |
| CN114377537A (en) * | 2022-01-14 | 2022-04-22 | 浙江工商大学 | Natural plant deodorant and preparation method and application thereof |
-
2018
- 2018-04-03 CN CN201810289500.XA patent/CN108902637A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840759A (en) * | 2019-10-15 | 2020-02-28 | 山东药品食品职业学院 | Anthocyanin bacteriostatic hand sanitizer and preparation method thereof |
| CN110685148A (en) * | 2019-10-23 | 2020-01-14 | 成工业制衣（苏州）有限公司 | Novel antibacterial textile fabric |
| CN114377537A (en) * | 2022-01-14 | 2022-04-22 | 浙江工商大学 | Natural plant deodorant and preparation method and application thereof |
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