CN108897128B - Microscopic imaging observation instrument for instantaneous dynamic process - Google Patents
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- 238000003384 imaging method Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000008569 process Effects 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 79
- 230000005540 biological transmission Effects 0.000 claims abstract description 18
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- 239000011521 glass Substances 0.000 claims description 4
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
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Abstract
The invention discloses an instantaneous dynamic process microscopic imaging observation instrument capable of automatically adding liquid, which comprises a full-automatic liquid adding unit, a relative microscopic unit, an imaging transmission unit and an intelligent control unit. The invention provides power through the mechanical movement of a motor propeller, a rotating arm swings to calibrate the position, and a reagent is added to a sample; then, utilizing the scattering or reflecting action of the light source on the surface of the sample, and obtaining the spatial information of the sample after the sample is amplified by the objective lens and the ocular lens; in addition, the information can be transmitted to a computer through the acquisition function of the imaging transmission unit camera, complete image information is formed through computer analysis software, and a picture is automatically shot according to set time. The invention can observe the change process of the sample at the moment of adding the reagent in real time, is suitable for observing the instantaneous dynamic process of reaction, and has the advantages of simple and easy operation, convenient maintenance and management, high automation degree and the like.
Description
Technical Field
The invention relates to the technical field of scientific research laboratory equipment, in particular to an instantaneous dynamic process microscopic imaging observation instrument capable of automatically adding liquid.
Background
The microscope is an instrument which enables human beings to observe a more delicate world, and since the invention is invented, the microscope is widely used in the scientific research fields of biology, physics, chemistry, medicine and the like, and the progress of modern science is greatly promoted. Microscopes can be classified into optical microscopes and electron microscopes according to their working principle and their constituent structure. The imaging principle of the optical microscope is that light is used as a medium, the light is irradiated on the surface of a sample through a condenser lens, and the light is amplified through an objective lens and an eyepiece so as to obtain amplified information of the sample; the imaging principle of the electron microscope is based on the electron optical principle, and takes the electron beam as a medium, and replaces the traditional light beam and optical lens by the electron beam and the electron lens. The device consists of a lens cone, a vacuum device and a power supply cabinet.
With the development of the existing technology, the digital optical microscope has been fully utilized, and the electronic signals are transmitted to the computer through the digital imaging device installed on the traditional optical microscope, and then complete image information is formed through the computer analysis software. Chinese patent application 201110322474.4 proposes a multifunctional digital microscope, which can be changed into a multifunctional novel microscope by replacing modular parts, so that the existing hardware base of the microscope is fully used. Chinese patent application 201410155453.1 proposes a digital microscope and a method of optimizing a work flow in a digital microscope, which can automatically analyze and evaluate the observation data of the monitoring sensor and generate control data; chinese patent application 201720714663.9 proposes a digital microscope, which can image more clearly and make the microscope structure more compact, and save the instrument space. Above relevant patent all made the perfect of certain degree to digital optical microscope, but also just promote the combination of current function, can't satisfy experimenter's more demands, like the reagent adds the real-time supervision of adding the sample, also can't reduce experimenter's film-making and read the piece volume to produce work load big, the repetition rate is high, work efficiency low grade problem.
Disclosure of Invention
The purpose is as follows: in order to overcome the defects in the prior art, the invention provides the microscopic imaging observation instrument capable of automatically adding liquid in the instantaneous dynamic process, which can observe the change of samples such as object surfaces, material surfaces, microbial communities and the like after adding the reagent in real time and fully automatically shoot and record the change images at different time points through the photographic device.
The technical scheme is as follows: in order to solve the technical problems, the technical scheme adopted by the invention is as follows:
an instantaneous dynamic process microscopic imaging observation instrument is characterized in that: the device comprises a full-automatic liquid adding unit, a relative microscopic unit, an imaging transmission unit and an intelligent control unit;
the full-automatic liquid adding unit comprises a spiral arm, a miniature liquid adding needle, a bracket, a motor propeller, a liquid taking needle and a liquid tank;
the relative microscopic unit comprises an ocular lens, an objective table, a condenser lens, a light collecting sheet and a base;
the imaging transmission unit comprises a camera and a computer;
the spiral arm is arranged at the upper end of the support and can rotate around the support by 360 degrees, the miniature liquid feeding needle is arranged on the spiral arm, a sample placing hole of the liquid tank is aligned below the liquid taking needle, the liquid taking needle is communicated with the miniature liquid feeding needle through a pipeline, and a motor propeller is arranged to provide power so that the pressure of the pipeline is increased and the liquid feeding amount of the miniature liquid feeding needle is controlled;
the bottom of the base is provided with a light source, the illumination can continuously adjust the brightness, and the light source and the centers of the objective lens, the condenser lens and the light collecting sheet are positioned on the same central axis, so that the maximum utilization of light energy can be realized; the scattering or reflecting action of the light source on the surface of the sample is utilized, and the spatial information of the sample is obtained after the sample is amplified by the objective lens and the ocular lens;
the image information of the sample is converted into an electronic signal by the acquisition function of the camera, the electronic signal is transmitted to a computer, and complete image information is formed by computer analysis software.
As a preferred scheme, the full-automatic liquid adding unit is positioned on the left side of the relative microscopic unit, and the bottom end of the bracket is connected to the base; a groove is reserved in the center of the spiral arm and used for withdrawing the micro liquid adding needle when liquid is not required to be added; the motor propeller is connected and positioned in the middle of the bracket; the liquid tank is disc-shaped and can rotate according to the program setting. Through the mechanical motion of motor propeller for pipeline pressure increases, absorbs the lifting by getting the liquid needle with reagent in the liquid groove, flows to miniature liquid needle department through the pipeline, and the spiral arm swing calibration position to accomplish the liquid feeding step. Furthermore, the support is made of aluminum alloy.
Preferably, the relative microscope unit further comprises a lens converter, a film clamp, a scale, an adjusting rotary wheel, a fine focusing screw and a coarse focusing screw; the slide holder for fixing the glass slide is arranged on the objective table, and the right side of the objective table comprises a scale and an adjusting rotary wheel; the fine and coarse focusing screws are located at the bottom of the right side of the microscope base. More preferably, the base is arc-shaped, an aluminum alloy or light metal frame is adopted, and a hole is reserved in the back of the base, so that the base is convenient to hold and move.
Preferably, the camera of the imaging transmission unit and the eyepiece are positioned at the same height, and the camera transmits the electronic signal to the computer through the data transmission line.
As a preferred scheme, the intelligent control unit comprises an image processing module, an automatic liquid adding module, an observation distance adjusting module, an automatic camera shooting control module and a time sequence recording module; the image processing module is used for measuring the size and the area of the whole sample or a part of the sample; the automatic liquid adding control module is used for controlling the full-automatic liquid adding unit and can set the sample adding sequence and the liquid taking amount of different reagents; the observation distance adjusting module can adjust the height of the microscope lens cone in a manual mode, and can also enable the lens cone to ascend or descend to a certain fixed height through a computer program; the automatic camera shooting control module and the time sequence recording module are used together, namely, the camera can shoot at set time by setting different recording time.
The invention is realized by the following principle: firstly, the pressure of a pipeline is increased through the mechanical movement of a motor propeller, a reagent in a liquid tank is sucked and lifted by a liquid taking needle and flows to the position of a micro liquid adding needle through the pipeline, and a rotating arm swings to calibrate the position, so that the liquid adding step is completed. Secondly, utilizing the scattering or reflecting action of the light source on the surface of the sample, and obtaining the spatial information of the sample after the sample is amplified by the objective lens and the ocular lens; in addition, the image information of the sample can be converted into an electronic signal through the acquisition function of the imaging transmission unit camera, the electronic signal is transmitted to a computer, and complete image information is formed through computer analysis software. The program software contains a time sequence recording module, can freely set time, completes automatic shooting, and observes and records the instant influence on the sample after adding the reagent and the change process along with the time in real time.
Has the advantages that: the microscopic imaging observation instrument capable of automatically adding liquid provided by the invention can be used for observing the changes of samples such as object surfaces, material surfaces, microbial communities and the like after adding the reagent in real time, and fully automatically shooting and recording the change images at different time points through the photographic device. Compared with the prior art, the method has the remarkable advantages that:
(1) the change process of the liquid adding instant sample can be observed in real time, and the method is suitable for observing the instant dynamic process of reaction;
(2) the reagent adding function is realized, and the reagent can be added to the sample as required, so that the tabletting amount of workers and errors caused in the tabletting process are reduced;
(3) the device has a photographing function, is connected with a computer, can realize real-time observation and communication of multiple people, stores an observation result in the computer, and leads out a picture for circulation;
(4) the system has automatic shooting and recording functions, and can automatically shoot in real time according to the set recording time after the observation visual field is adjusted, so that the observation waiting time is saved;
(5) contains a plurality of automatic modules, and is more convenient to use.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of the structure of the fully automatic liquid adding unit of the present invention.
In the figure: 1. a full-automatic liquid adding unit; 2. a relative microscope unit; 3. an imaging microscope unit; 5. A swing arm; 6. a micro liquid adding needle; 7. a support; 8. a motor propeller; 9. a liquid taking needle; 10. a liquid tank; 11. an eyepiece; 12. a lens converter; 13. an objective lens; 14. a film clip; 15. a scale; 16. an object stage; 17. a condenser lens; 18. adjusting the rotary wheel; 19. fine-focus helicoids; 20. coarsely focusing the spiral; 21. a light collecting sheet; 22. a base; 23. a computer; 24. a data transmission line; 25. a camera.
Detailed Description
The present invention will be further described with reference to the following examples.
As shown in fig. 1 to 2, an observation apparatus for microscopic imaging in instantaneous dynamic process capable of automatically adding liquid comprises a full-automatic liquid adding unit 1 consisting of a swing arm 5, a micro liquid adding needle 6, a bracket 7, a motor propeller 8, a liquid taking needle 9 and a liquid tank 10, a relative microscopic unit 2 consisting of an ocular lens 11, a lens converter 12, an objective lens 13, a film clamp 14, a scale 15, an objective table 16, a condenser lens 17, an adjusting swing wheel 18, a fine focusing screw 19, a coarse focusing screw 20, a light collecting sheet 21 and a base 22, an imaging transmission unit 3 consisting of a camera 25, a data transmission line 24 and a computer 23, and an intelligent control unit consisting of an image processing module, an automatic liquid adding module, an observation distance adjusting module, an automatic camera shooting control module and a time sequence recording module. Wherein:
the full-automatic liquid adding unit 1 is positioned on the left side relative to the microscope unit 2, the rotating arm 5 is fixed at the upper end of the bracket 7, 360-degree rotation can be realized, a groove is reserved in the center, and the micro liquid adding needle 6 is retracted when liquid adding is not needed; the motor propeller 8 is positioned in the middle of the bracket 7 and is fixedly connected with the bracket 7, and the other end of the motor propeller is connected with the liquid taking needle 9; the lower part of the liquid taking needle 9 is right opposite to a sample placing hole of the liquid tank 10, and the liquid tank 10 is in a disc shape and can rotate according to the program setting.
The base 22 of the relative microscope unit 2 is arc-shaped, and a hole is reserved on the back of the base, so that the base is convenient to hold by hand for moving; the bottom of the base 22 is provided with a light source which is positioned on the same central axis with the centers of the objective lens 13, the condenser lens 17 and the light collecting sheet 21, so that the maximum utilization of light energy can be realized; a slide holder 14 for fixing a slide glass is arranged on the objective table 16, and the right side of the objective table contains a scale 15 and an adjusting rotary wheel 18; the fine focus screw 19 and the coarse focus screw 20 are located at the bottom right side of the microscope base 22.
The camera 25 of the imaging transmission unit 3 and the eyepiece 11 are located at the same height, the acquisition function of the camera 25 converts the image information of the sample into an electronic signal, and the electronic signal is transmitted to the computer 23 through the data transmission line 24, and then the complete image information is formed through computer analysis software.
The intelligent control unit mainly comprises five modules, and the image processing module is used for measuring the size and the area of the whole sample or a part of the sample; the automatic liquid adding control module is used for controlling the automatic liquid adding unit and can set the sample adding sequence and the liquid taking amount of different reagents; the observation distance adjusting module can adjust the height of the microscope lens cone in a manual mode, and can also enable the lens cone to ascend or descend to a certain fixed height through a computer program; the automatic camera control module and the time sequence recording module are used together, that is, by setting different recording times, the camera 25 takes pictures at the set time.
The specific operation steps of the present invention are further described below with reference to fig. 1 and 2:
the invention provides a construction method of an instantaneous dynamic process microscopic imaging observation instrument capable of automatically adding liquid, which comprises a full-automatic liquid adding unit, a relative microscopic unit, an imaging transmission unit and an intelligent control unit.
The method comprises the following steps: the support 7 is constructed by aluminum alloy, the spiral arm 5 is fixed at the upper end of the support 7, 360-degree rotation can be realized, a groove is reserved in the center, and the micro liquid adding needle 6 is retracted when liquid is not required to be added;
step two: the motor propeller 8 is positioned in the middle of the bracket 7 and is fixedly connected with the bracket 7, and the other end of the motor propeller is connected with the liquid taking needle 9; a sample placing hole of the liquid tank 10 is rightly arranged below the liquid taking needle 9, and the liquid tank 10 is disc-shaped and can rotate according to the program setting;
step three: the base 22 is arc-shaped, an aluminum alloy or light metal frame is adopted, and a hole is reserved on the back of the base, so that the microscope can be conveniently held by a hand to move; the bottom of the base 22 is provided with a light source, an electric light source is adopted, the illumination can continuously adjust the brightness, and the light source and the centers of the objective lens 13, the condenser lens 17 and the light collecting sheet 21 are positioned on the same central axis, so that the maximum utilization of light energy can be realized;
step four: a slide holder 14 for fixing a slide glass is arranged on the objective table 16, and the right side of the objective table contains a scale 15 and an adjusting rotary wheel 18; the fine focusing screw 19 and the coarse focusing screw 20 are positioned at the bottom of the right side of the microscope base 22;
step five: the camera 25 and the ocular lens 11 are positioned at the same height and used for shooting image information of a sample, and one end of the data transmission line 24 is connected with the camera 25, and the other end is connected with the computer 23 and used for transmitting signals.
The above description is only of the preferred embodiments of the present invention, and it should be noted that: it will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the principles of the invention and these are intended to be within the scope of the invention.
Claims (8)
1. An instantaneous dynamic process microscopic imaging observation instrument is characterized in that: the device comprises a full-automatic liquid adding unit, a relative microscopic unit, an imaging transmission unit and an intelligent control unit;
the full-automatic liquid adding unit comprises a spiral arm, a miniature liquid adding needle, a bracket, a motor propeller, a liquid taking needle and a liquid tank;
the relative microscopic unit comprises an ocular lens, an objective table, a condenser lens, a light collecting sheet and a base;
the imaging transmission unit comprises a camera and a computer;
the spiral arm is arranged at the upper end of the support and can rotate around the support by 360 degrees, the miniature liquid feeding needle is arranged on the spiral arm, a sample placing hole of the liquid tank is aligned below the liquid taking needle, the liquid taking needle is communicated with the miniature liquid feeding needle through a pipeline, and a motor propeller is arranged to provide power so that the pressure of the pipeline is increased and the liquid feeding amount of the miniature liquid feeding needle is controlled;
the bottom of the base is provided with a light source which is positioned on the same central axis with the centers of the objective lens, the condenser lens and the light collecting sheet, and the space information of the sample is obtained by utilizing the scattering or reflecting action of the light source on the surface of the sample and the amplification of the objective lens and the eyepiece;
the image information of the sample is converted into an electronic signal by the acquisition function of the camera, the electronic signal is transmitted to a computer, and complete image information is formed by computer analysis software.
2. The transient dynamic process microscopic imaging viewer of claim 1, wherein: the full-automatic liquid adding unit is positioned on the left side of the relative microscopic unit.
3. The transient dynamic process microscopic imaging viewer of claim 1, wherein: the bottom end of the support is connected to the base.
4. The transient dynamic process microscopic imaging viewer of claim 1, wherein: a groove is reserved in the center of the spiral arm and used for withdrawing the micro liquid adding needle when liquid is not required to be added; the motor propeller is connected and positioned in the middle of the bracket.
5. The transient dynamic process microscopic imaging viewer of claim 1, wherein: the liquid tank is of a disc-shaped structure and can rotate according to program setting.
6. The transient dynamic process microscopic imaging viewer of claim 1, wherein: the relative microscopic unit also comprises a lens converter, a film clamp, a scale, an adjusting rotary wheel, a fine focusing screw and a coarse focusing screw; the slide holder for fixing the glass slide is arranged on the objective table, and the right side of the objective table comprises a scale and an adjusting rotary wheel; the fine and coarse focusing screws are located at the bottom of the right side of the microscope base.
7. The transient dynamic process microscopic imaging viewer of claim 1, wherein: the base is arc-shaped, and a hole is reserved in the back of the base, so that the base is convenient to hold and move.
8. The transient dynamic process microscopic imaging viewer of claim 1, wherein: the camera of the imaging transmission unit and the eyepiece are positioned at the same height, and the camera transmits the electronic signal to the computer through the data transmission line.
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| WO2013152239A1 (en) * | 2012-04-04 | 2013-10-10 | National University Of Singapore | Cryo-preparation systems and methods for near-instantaneous vitrification of biological samples |
| CN103353670A (en) * | 2013-08-01 | 2013-10-16 | 陈松 | Full-automatic electrically-controlled microscope sample bench |
| CN204462166U (en) * | 2015-01-19 | 2015-07-08 | 苏州迈迪达医疗器械科技有限公司 | A kind of copra analysis instrument |
| CN206848132U (en) * | 2016-10-31 | 2018-01-05 | 杭州昕龙医疗科技有限公司 | Sample microscopy analytical equipment |
| CN106383242B (en) * | 2016-10-31 | 2018-10-26 | 杭州昕龙医疗科技有限公司 | Sample analyser |
| CN107976796B (en) * | 2017-12-23 | 2024-03-15 | 新昌县七星街道新伟机械厂 | Biological microscope structure for plant quarantine |
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Effective date of registration: 20240218 Address after: 210000, South 1009-25, Chuangzhi Building, No.1 Xichun Road, Yuhuatai District, Nanjing City, Jiangsu Province, China Patentee after: Jiangsu Jiuzhang Water Conservancy and Environmental Technology Co.,Ltd. Country or region after: China Address before: 211100 No. 8 West Buddha Road, Jiangning District, Jiangsu, Nanjing Patentee before: HOHAI University Country or region before: China |