CN108896675A - 一种l-瓜氨酸-dl-苹果酸的高效液相检测方法 - Google Patents
一种l-瓜氨酸-dl-苹果酸的高效液相检测方法 Download PDFInfo
- Publication number
- CN108896675A CN108896675A CN201810737037.0A CN201810737037A CN108896675A CN 108896675 A CN108896675 A CN 108896675A CN 201810737037 A CN201810737037 A CN 201810737037A CN 108896675 A CN108896675 A CN 108896675A
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- China
- Prior art keywords
- citrulline
- malic acid
- liquid phase
- efficient liquid
- detection method
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- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 239000007791 liquid phase Substances 0.000 title claims abstract description 22
- 239000012071 phase Substances 0.000 claims abstract description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002904 solvent Substances 0.000 claims abstract description 8
- VWDWKYIASSYTQR-YTBWXGASSA-N sodium;dioxido(oxo)azanium Chemical compound [Na+].[O-][15N+]([O-])=O VWDWKYIASSYTQR-YTBWXGASSA-N 0.000 claims abstract description 6
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 4
- RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 claims description 46
- 239000000243 solution Substances 0.000 claims description 28
- 239000013558 reference substance Substances 0.000 claims description 25
- 229960002173 citrulline Drugs 0.000 claims description 23
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 23
- 238000012417 linear regression Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 241000219112 Cucumis Species 0.000 claims description 5
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 4
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 3
- 239000012535 impurity Substances 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- -1 Propylhomoserin-DL-malic acid Chemical compound 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 102000008299 Nitric Oxide Synthase Human genes 0.000 description 2
- 108010021487 Nitric Oxide Synthase Proteins 0.000 description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000010812 external standard method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 208000037157 Azotemia Diseases 0.000 description 1
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 201000001880 Sexual dysfunction Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001370 alpha-amino acid derivatives Chemical class 0.000 description 1
- 235000008206 alpha-amino acids Nutrition 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 230000002272 anti-calculus Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 229960003624 creatine Drugs 0.000 description 1
- 239000006046 creatine Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 230000004199 lung function Effects 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960003753 nitric oxide Drugs 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 231100000872 sexual dysfunction Toxicity 0.000 description 1
- 230000036299 sexual function Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 150000003628 tricarboxylic acids Chemical class 0.000 description 1
- 230000004143 urea cycle Effects 0.000 description 1
- 208000009852 uremia Diseases 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
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CN201810737037.0A CN108896675B (zh) | 2018-07-06 | 2018-07-06 | 一种l-瓜氨酸-dl-苹果酸的高效液相检测方法 |
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CN201810737037.0A CN108896675B (zh) | 2018-07-06 | 2018-07-06 | 一种l-瓜氨酸-dl-苹果酸的高效液相检测方法 |
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CN108896675A true CN108896675A (zh) | 2018-11-27 |
CN108896675B CN108896675B (zh) | 2020-10-16 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112198236A (zh) * | 2019-12-05 | 2021-01-08 | 湖北远大生物技术有限公司 | 一种瓜氨酸原料中瓜氨酸含量的检测方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1697658A (zh) * | 2003-05-21 | 2005-11-16 | 株式会社琉球生物资源开发 | 发酵处理物及其制造方法 |
CN104807924A (zh) * | 2014-01-28 | 2015-07-29 | 黑龙江天行健医药科技开发有限公司 | 门冬氨酸鸟氨酸原料及制剂特定杂质检测方法 |
US20160000921A1 (en) * | 2013-03-15 | 2016-01-07 | Merck Sharp & Dohme Corp. | Ceftolozane antibiotic compositions |
CN107007838A (zh) * | 2016-09-30 | 2017-08-04 | 南京优科制药有限公司 | 一种含有马来酸依那普利、叶酸和酸稳定剂的药物组合物 |
CN107064508A (zh) * | 2017-04-21 | 2017-08-18 | 深圳大学 | 辅助结直肠癌早期诊断及预后监测的分子标志物及其应用 |
-
2018
- 2018-07-06 CN CN201810737037.0A patent/CN108896675B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1697658A (zh) * | 2003-05-21 | 2005-11-16 | 株式会社琉球生物资源开发 | 发酵处理物及其制造方法 |
US20160000921A1 (en) * | 2013-03-15 | 2016-01-07 | Merck Sharp & Dohme Corp. | Ceftolozane antibiotic compositions |
CN104807924A (zh) * | 2014-01-28 | 2015-07-29 | 黑龙江天行健医药科技开发有限公司 | 门冬氨酸鸟氨酸原料及制剂特定杂质检测方法 |
CN107007838A (zh) * | 2016-09-30 | 2017-08-04 | 南京优科制药有限公司 | 一种含有马来酸依那普利、叶酸和酸稳定剂的药物组合物 |
CN107064508A (zh) * | 2017-04-21 | 2017-08-18 | 深圳大学 | 辅助结直肠癌早期诊断及预后监测的分子标志物及其应用 |
Non-Patent Citations (1)
Title |
---|
李小平 等: "果汁中9种有机酸的反相高效液相色谱法测定", 《中国卫生检验杂志》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112198236A (zh) * | 2019-12-05 | 2021-01-08 | 湖北远大生物技术有限公司 | 一种瓜氨酸原料中瓜氨酸含量的检测方法 |
CN112198236B (zh) * | 2019-12-05 | 2022-04-22 | 湖北远大生物技术有限公司 | 一种瓜氨酸原料中瓜氨酸含量的检测方法 |
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CN108896675B (zh) | 2020-10-16 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A High Performance Liquid Phase Detection Method for L-Citrulline-DL-Malic Acid Effective date of registration: 20230418 Granted publication date: 20201016 Pledgee: Hebei Ningjin Rural Commercial Bank Co.,Ltd. Pledgor: JING JING PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980038366 |
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Date of cancellation: 20231114 Granted publication date: 20201016 Pledgee: Hebei Ningjin Rural Commercial Bank Co.,Ltd. Pledgor: JING JING PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980038366 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A High Performance Liquid Phase Detection Method for L-Citrulline-DL-Malic Acid Effective date of registration: 20231204 Granted publication date: 20201016 Pledgee: Hebei Ningjin Rural Commercial Bank Co.,Ltd. Pledgor: JING JING PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980069369 |
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