CN108893558B - Mulberry juice stock solution antiviral experimental method and device - Google Patents
Mulberry juice stock solution antiviral experimental method and device Download PDFInfo
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- CN108893558B CN108893558B CN201810505648.2A CN201810505648A CN108893558B CN 108893558 B CN108893558 B CN 108893558B CN 201810505648 A CN201810505648 A CN 201810505648A CN 108893558 B CN108893558 B CN 108893558B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
Abstract
The invention discloses an antiviral experimental method of mulberry juice stock solution, which comprises the steps of preparing the mulberry juice stock solution, adding deionized water to obtain 10 times of dilution solution of the mulberry juice, injecting 0.4g of 10 times of dilution solution of the mulberry juice into an experimental sprayer I, and injecting the same amount of deionized water into an experimental sprayer II. The invention also discloses a mulberry liquid stock solution antiviral experimental device, which comprises an experimental dish, wherein two identical experimental grooves are concavely arranged on the top surface of the experimental dish, a sleeve is movably arranged in each experimental groove through a threaded structure, the sleeve is fixedly arranged at the bottom end of a cover plate, a sealing opening is arranged at the top end of the cover plate in a penetrating manner, and a rubber plug is fixedly arranged in the sealing opening. The extracted mulberry liquid stock solution is diluted by 10 times, compared with the deionized water control with the same quantity, the mulberry liquid diluted by 10 times has stronger antiviral property to influenza virus H1N1, and meanwhile, the extraction cost of the mulberry liquid is far lower than that of the existing synthetic drugs, so that the mulberry liquid is convenient to put into use in large quantity.
Description
Technical Field
The invention relates to the technical field of antiviral experiments, in particular to an antiviral experiment method and device for mulberry juice stock solution.
Background
The virus is a non-cell life form, which is composed of a long nucleic acid chain and a protein shell, and has no own metabolic mechanism and no enzyme system. Therefore, the virus leaves the host cell and becomes a chemical substance which does not have any vital activity and can not independently propagate. Once inside the host cell, it can use the materials and energy in the cell and the ability to replicate, transcribe and translate to produce a new generation of virus as it does with the genetic information contained in its own nucleic acid.
Most of the existing antiviral drugs are prepared by synthesis at present, but extracts in some plants in nature also have certain antiviral properties, and honeysuckle, polygonum cuspidatum, isatis root, eucalyptus robusta, crape myrtle, schisandra chinensis, cherokee rose and the like are common, and then in the prior art, no research on the antiviral properties of the mulberry juice is provided, so that an antiviral experimental method and a device for the mulberry juice stock solution are provided for solving the problems.
Disclosure of Invention
The invention aims to provide an antiviral experimental method and device for mulberry juice stock solution, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an antiviral experimental method for mulberry juice stock solution comprises the following steps:
s1: preparing a mulberry liquid stock solution, adding deionized water to obtain a mulberry liquid 10-time diluent, injecting 0.4g of mulberry liquid 10-time diluent into an experiment sprayer I, and injecting the same amount of deionized water into an experiment sprayer II;
s2: respectively putting two untreated canine kidney epidermis into two experimental grooves of an experimental dish, respectively carrying out virus infection treatment on the two canine kidney epidermis, wherein the virus strain is influenza virus H1N1, then rotatably sealing a cover plate on the experimental grooves, and putting the experimental dish into a constant temperature box for inoculation;
s3: taking out the experiment vessel in the incubator, and determining the virus infection prices on the epidermis of the two canine kidneys in the experiment vessel;
s4: after the determination, inserting a nozzle of an experiment sprayer I of S1 into a sealing port at the upper end of a cover plate, spraying mulberry stock solution on the canine kidney epidermis in the experiment groove, and spraying the same amount of deionized water on the canine kidney epidermis through an experiment sprayer II;
s5: putting the experiment dish treated in the step S4 into the constant temperature box again for cell induction;
s6: the treated dishes in S5 were removed and the viral activity values on the epidermis of both canine kidneys were determined.
Preferably, the inoculation temperature in the incubator in S2 is 24-26 ℃ and the inoculation time is 1.5-2.5 hours.
Preferably, the cell induction temperature in S5 is 36-38 deg.C, the carbon dioxide concentration in the incubator is 4-6%, and the cell induction time is 70-75 hours.
The utility model provides an antiviral experimental apparatus of mulberry wood liquid stoste, includes the laboratory dish, the laboratory dish top surface indent is equipped with two identical experimental grooves, the experimental inslot is equipped with the sleeve through the helicitic texture activity, sleeve fixed mounting is in the apron bottom, the apron top is run through and is equipped with the sealing, the fixed rubber buffer that is equipped with in the sealing.
Preferably, the cover plate is a transparent glass cover plate, and a sealing gasket is fixedly arranged at the bottom end of the cover plate.
Compared with the prior art, the invention has the beneficial effects that: the extracted mulberry liquid stock solution is diluted by 10 times, compared with the deionized water control with the same quantity, the mulberry liquid diluted by 10 times has stronger antiviral property to influenza virus H1N1, and meanwhile, the extraction cost of the mulberry liquid is far lower than that of the existing synthetic drugs, so that the mulberry liquid is convenient to put into use in large quantity.
Drawings
FIG. 1 is a schematic view of the antiviral experimental apparatus of the present invention.
In the figure: 1 laboratory dish, 101 experimental groove, 2 apron, 201 sleeve, 202 sealing washer, 203 sealing port, 204 rubber buffer.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution: an antiviral experimental method for mulberry juice stock solution comprises the following steps:
s1: preparing a mulberry liquid stock solution, adding deionized water to obtain a mulberry liquid 10-time diluent, injecting 0.4g of mulberry liquid 10-time diluent into an experiment sprayer I, and injecting the same amount of deionized water into an experiment sprayer II;
s2: respectively placing two untreated canine kidney epidermis into two experimental grooves 101 of an experimental dish 1, then respectively carrying out virus infection treatment on the two canine kidney epidermis, wherein the virus strain is influenza virus H1N1, then rotationally sealing a cover plate 2 on the experimental grooves 101, and placing the experimental dish 1 into a constant temperature box for inoculation;
s3: taking out the experiment vessel 1 in the incubator, and measuring the virus infection prices on the canine kidney epidermis in the experiment vessel;
s4: after the determination, inserting a nozzle of an experiment sprayer I of S1 into a sealing port 203 at the upper end of a cover plate 2, spraying mulberry stock solution on the epidermis of the kidney of the dog in the experiment groove 101, and spraying deionized water with the same amount on the epidermis of the kidney of the other dog through an experiment sprayer II;
s5: putting the experiment dish 1 treated in the S4 into the constant temperature box again for cell induction;
s6: the test dish 1 treated in S5 was removed, and the viral activity values on the epidermis of two canine kidneys were measured, respectively.
Further, the inoculation temperature in the incubator in S2 is 24-26 ℃, and the inoculation time is 1.5-2.5 hours.
Furthermore, the cell induction temperature in S5 is 36-38 ℃, the concentration of carbon dioxide in the incubator is 4-6%, and the cell induction time is 70-75 hours.
The utility model provides an antiviral experimental apparatus of mulberry liquid stoste, includes experiment dish 1, and 1 top surface indent of experiment dish is equipped with two identical experiment grooves 101, is equipped with sleeve 201 through the helicitic texture activity in the experiment groove 101, and sleeve 201 fixed mounting is in 2 bottoms of apron, and 2 tops of apron run through and are equipped with sealed mouthful 203, and sealed mouthful 203 internal fixation is equipped with rubber buffer 204.
Further, the cover plate 2 is a transparent glass cover plate, and a sealing gasket 202 is fixedly arranged at the bottom end of the cover plate.
According to the above procedure, the data as shown in the following table can be obtained:
wherein:
antiviral activity value (ISO standard) = control sample virus infection value-sample virus infection value
Antiviral activity value (JIS standard) = initial viral infection value-sample viral infection value
ISO standard:
antiviral activity value > =2, is antiviral;
the antiviral activity value > =3, and the antiviral activity is stronger.
JIS standard:
antiviral activity value > =3, has antiviral activity.
According to either of the above ISO standard and JIS standard, we can know that the mulberry liquid 10-fold diluted liquid has antiviral activity.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. An antiviral experimental method for mulberry juice stock solution is characterized by comprising the following steps:
s1: preparing a mulberry liquid stock solution, adding deionized water to obtain a mulberry liquid 10-time diluent, injecting 0.4g of mulberry liquid 10-time diluent into an experiment sprayer I, and injecting the same amount of deionized water into an experiment sprayer II;
s2: respectively putting two untreated canine kidney epidermis into two experimental grooves (101) of an experimental dish (1), then respectively carrying out virus infection treatment on the two canine kidney epidermis, wherein the virus strain is influenza virus H1N1, then rotatably sealing a cover plate (2) on the experimental grooves (101), and putting the experimental dish (1) into a constant temperature box for inoculation;
s3: taking out the experiment vessel (1) in the incubator, and measuring the virus infection prices on the epidermis of the two canine kidneys in the experiment vessel;
s4: after the determination, inserting a nozzle of an experiment sprayer I of S1 into a sealing port (203) at the upper end of a cover plate (2), spraying mulberry stock solution on the canine kidney epidermis in the experiment groove (101), and spraying the same amount of deionized water on the canine kidney epidermis through an experiment sprayer II;
s5: putting the experiment dish (1) treated in the step S4 into the constant temperature box again for cell induction;
s6: the treated plate (1) in S5 was taken out, and the virus activity values on the epidermis of two canine kidneys were measured, respectively.
2. The antiviral experimental method of mulberry sap stock solution as claimed in claim 1, wherein the antiviral experimental method comprises the following steps: the inoculation temperature in the incubator in S2 is 24-26 ℃, and the inoculation time is 1.5-2.5 hours.
3. The antiviral experimental method of mulberry sap stock solution as claimed in claim 1, wherein the antiviral experimental method comprises the following steps: the cell induction temperature in S5 is 36-38 deg.C, the carbon dioxide concentration in the incubator is 4-6%, and the cell induction time is 70-75 hr.
4. The antiviral experimental device of mulberry juice stock solution used in the claim 1 is characterized in that: including experiment dish (1), experiment dish (1) top surface indent is equipped with two identical experiment grooves (101), be equipped with sleeve (201) through the helicitic texture activity in experiment groove (101), sleeve (201) fixed mounting is in apron (2) bottom, apron (2) top is run through and is equipped with sealing opening (203), sealing opening (203) internal fixation is equipped with rubber buffer (204).
5. The antiviral experimental device of mulberry sap stock solution of claim 4, characterized in that: the cover plate (2) is a transparent glass cover plate, and a sealing gasket (202) is fixedly arranged at the bottom end of the cover plate.
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