CN108887241A - Application and diaphorina citri trapper of the special light source in terms of traping diaphorina citri - Google Patents

Abstract

The invention discloses the application of a special light source in trapping citrus psyllids and a trap for citrus psyllids. The wavelength of the special light source is 409.9nm-511.6nm. Different wavelengths of light have different trapping rates for citrus psyllids. For light sources with a wavelength of 409.9nm to 511.6nm, citrus psyllids have the highest response index and positive phototaxis especially to light sources from 409.9nm to 469.2nm or 491.6nm to 511.6nm. index. When the light source of said wavelength is used, the effect of trapping citrus psyllids by light can be greatly improved; meanwhile, the wavelength of said special light source is single, the pertinence is strong, and the specificity of trapping citrus psyllids is strong. In particular, beneficial insects are less harmful. Using a light source with a specific wavelength to trap citrus psyllids can significantly improve the trapping effect of the trap light, improve the control effect of the trap on citrus psyllids, reduce the use of chemical pesticides, reduce pesticide residues in citrus, and delay the impact of citrus psyllids. The emergence of chemical pesticide resistance and reduce the pollution of chemical pesticides to the environment.

Description

Application of special light source in trapping citrus psyllid and citrus psyllid trap

technical field

The invention relates to the technical field of preventing and controlling citrus psyllid adults, and more particularly relates to the application of a special light source in trapping citrus psyllids and a citrus psyllid trap.

Background technique

Light trapping technology is a physical control method that uses the behavior of insects to move toward or away from the light source to encourage insects to gather at a fixed location for centralized elimination. At present, there are many studies on the phototaxis behavior of insects, but the phototaxis behavior of insects is affected by many aspects, and the phototaxis behavior includes positive phototaxis and negative phototaxis, and negative phototaxis is often ignored by people. In addition, the phototaxis behavior of insects also lacks a unified standard.

Citrus psyllids belong to Hemiptera Psyllidae, and are important pests on Rutaceae plants such as citrus, lemon, yellow bark, and mulberry. The citrus psyllid sucks and sucks. The adults disperse and suck on the leaves and shoots, and the nymphs cluster on the new shoots, shoots and new leaves, causing the shoots and shoots to yellow, shrink, dry, and the new leaves are twisted and deformed and easy to fall off. , seriously affecting plant growth. The white honeydew secreted by the nymphs during the feeding process adheres to the surface of plant leaves, affects the light and function of plants, and causes sooty spot disease at the same time. Compared with direct feeding hazards, citrus psyllids, as an important vector of citrus H.

Citrus psyllids have strong photochromatic behavior. At present, yellow boards are used to trap citrus psyllids to some extent, but yellow boards can only be used during the day, and yellow boards have no trapping effect on citrus psyllids at night. At present, there are few studies on the prevention and treatment of citrus psyllids with light trapping technology, the trapping rate is poor, and the specificity is not good. The effect of trapping citrus psyllids with lights is not obvious.

Contents of the invention

The purpose of the present invention is to improve the trapping effect and specificity effect of light trapping citrus psyllids, and provides the application of special light source in trapping citrus psyllids. The present invention selects a light source with a specific wavelength, and the citrus psyllid has the highest total photoresponse index and the highest positive phototaxis to the light source. When the wavelength is used, the effect of trapping the citrus psyllid can be greatly improved; at the same time, the special light source The wavelength is single, the pertinence is strong, and the singleness of trapping citrus psyllids is strong, and it is less harmful to other insects, especially beneficial insects.

Another object of the present invention is to provide a citrus psyllid trap. Using a light source with a specific wavelength to trap citrus psyllids can significantly improve the trapping effect of the trap light, improve the control effect of the trap on citrus psyllids, reduce the use of chemical pesticides, reduce pesticide residues in citrus, and delay the impact of citrus psyllids. The emergence of chemical pesticide resistance and reduce the pollution of chemical pesticides to the environment.

Above-mentioned purpose of the present invention is achieved by following scheme:

Application of a special light source in trapping citrus psyllids, the wavelength of the special light source is 409.9nm-511.6nm.

Through multiple experiments, the inventor found that the trapping rate of citrus psyllids differs greatly with light of different wavelengths. When the wavelength is 409.9nm-511.6nm, the total response index and positive phototaxis index of citrus psyllids to light sources are the highest. Preferably, trapping citrus psyllids with the light source of said wavelength can significantly improve the trapping effect on citrus psyllids.

Preferably, the wavelength of the dedicated light source is 409.9nm-469.2nm or 491.6nm-511.6nm.

More preferably, the wavelength of the dedicated light source is 419.9±10 nm, 434.7±10 nm, 459.2±10 nm or 501.6±10 nm.

Preferably, the dedicated light source may be a combination of multiple wavelengths.

Preferably, the illumination intensity of the dedicated light source is above 300 μw/cm ² /s.

Preferably, the illumination intensity of the dedicated light source is 800-1000 μw/cm ² /s; more preferably, the illumination intensity of the dedicated light source is 1000 μw/cm ² /s.

Preferably, the dedicated light source may be an LED light source or a fluorescent lamp. As a fourth-generation new light source, LED lights have a narrow wavelength range and a single light color, which is highly targeted for trapping target pests. LED lights have high brightness, low energy consumption, and long life.

At the same time, the invention also protects a trap for citrus psyllids, the wavelength of the light source of the trap is 409.9nm-511.6nm.

Preferably, the wavelength of the light source of the trap is 409.9nm-469.2nm or 491.6nm-511.6nm.

More preferably, the wavelength of the dedicated light source is 419.9±10 nm, 434.7±10 nm, 459.2±10 nm or 501.6±10 nm.

The present invention also protects the trap prepared by a special light source at the same time, the trap includes a light source, a power supply and a timing device, a sticky yellow plate and a color plate support; the power supply is used to supply power to the light source and the timing device; the light source, power supply . The timing device is installed on the upper end of the swatch support; the viscous yellow plate is arranged at the lower end of the swatch support, below the light source.

Because the citrus psyllid has chromatism, the citrus psyllid will tend to fly to the yellow board, and when it stays on the sticky yellow board, it cannot fly away from the yellow board and can only stay on the yellow board. At night, the power supply supplies power to the light source within the time period set by the timing device. Due to the phototaxis and coloration of the citrus psyllid, the citrus psyllid continuously flies to the sticky yellow board, thereby being stuck and staying on the yellow board. To achieve the effect of trapping citrus psyllids. Combining phototaxis and coloration of citrus psyllid can achieve better trapping effect.

Preferably, the light source is an LED light source or a fluorescent lamp.

Preferably, the trap also includes a waterproof baffle; the waterproof baffle is installed on the top of the color plate support, wherein the power supply and timing device are arranged inside the waterproof baffle, and the light source is arranged under the waterproof baffle. The waterproof baffle is used to protect the power supply, timing device and light source from being damaged by rainwater.

The light source is installed under the waterproof baffle to prevent the light source from being knocked during installation or transportation; or damaged by birds and other animals during use, so as to prolong the service life of the light source.

Preferably, the color board support is provided with a card slot for clamping the adhesive yellow board; the power supply is a lithium battery. When the viscous yellow board has been used for a long time and the stickiness of the citrus psyllid cannot be well adhered, or there are too many citrus psyllids stuck, it can be easily replaced with a new viscous yellow board. The swatch support can be made into a rod shape or a plate shape, and the specific shape and structure can be adjusted according to the requirements of use or the restrictions of the use environment.

Preferably, the trap is also provided with a solar panel; the solar panel is electrically connected to the light source and the timing device, and installed on the top of the waterproof baffle.

Preferably, the trap is also provided with a hook, which is arranged above the solar panel.

Compared with the prior art, the present invention has the following beneficial effects:

There are great differences in the trapping rate of citrus psyllids under different wavelengths of light. When the wavelength is 409.9nm～511.6nm, especially when the wavelength is 419.9±10nm, 434.7±10nm, 459.2±10nm or 501.6±10nm, citrus The total response index and positive phototaxis index of psyllids to light sources are the best, and trapping citrus psyllids by using the single wavelength as light source or the combination of single wavelengths as light sources can significantly improve the trapping effect on citrus psyllids. At the same time, the wavelength of the special light source is single, the pertinence is strong, the singleness of trapping citrus psyllids is strong, and there is no harm to other insects, especially beneficial insects.

Using a light source with a specific wavelength to trap citrus psyllids can significantly improve the trapping effect of the trap light, improve the control effect of the trap on citrus psyllids, reduce the use of chemical pesticides, reduce pesticide residues in citrus, and delay the impact of citrus psyllids. The emergence of chemical pesticide resistance and reduce the pollution of chemical pesticides to the environment.

Description of drawings

FIG. 1 is a schematic structural view of the measurement device used to detect the positive/negative phototaxis behavior of small insects in Example 1.

Fig. 2 is a schematic structural view of the front view of the trap described in Example 2.

Fig. 3 is a structural schematic diagram of a side view of the trap described in Example 2.

Figure 4 is the result of trapping citrus psyllids in Example 2.

Detailed ways

The present invention will be further described in detail below in conjunction with specific embodiments, which are only used to explain the present invention, and are not intended to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified.

Example 1 Detection of the positive and negative phototaxis behavior of adults of citrus psyllids to light sources of different wavelengths

1. Experimental materials

(1) Source of tested insects: Adult citrus psyllids were obtained from the Entomology Department of South China Agricultural University. They were bred on Gourilla japonica. The breeding temperature was 26±1ºC and the relative humidity was 60±5%.

(2) Light sources for testing: 9 kinds of wavelengths: 365.0±10nm, 419.9±10nm, 434.7±10nm, 459.2±10nm, 501.6±10nm, 610.6±10nm, 691.5±10nm, 729.0±10nm, 780.0±10nm Provided by Hunan Benye Green Prevention and Control Technology Co., Ltd.

(3) Testing device: The schematic diagram of the testing device is shown in FIG. 1 . The detection device is Y-shaped, wherein 1, 4, and 7 are black non-reflective sponge plugs; 2 is a negative phototaxis reaction chamber made of quartz glass, cylindrical, with an outer diameter of 2.2 cm and an inner diameter of 2.0 cm; 3 and 8 are Black non-reflective foam paper shading cover; 5 is the positive phototaxis reaction chamber, made of quartz glass, cylindrical, with an outer diameter of 2.2cm, and an inner diameter of 2.0cm; 6 is the habitat chamber, made of quartz glass, cylindrical, with an outer diameter of 2.2cm , with an inner diameter of 2.0cm; 9 is a single-wavelength LED light source; 10 is a light source regulator; 11 is a power cord; the angle between 2 and 5 is 60º, and the angle between 6 and 6 is 150º; the vertical distance between 5 and 9 is 30cm.

2. Experimental method

After the citrus psyllid adults eclosion, the male and female were mixed for one to two weeks for the experiment, and the experiment time was from 6:00 to 11:00 pm every day. At the beginning of the experiment, 20 citrus psyllids were randomly selected, subjected to dark adaptation for 30 minutes, and then placed in habitat chamber 6. Turn on the LED light source 9, adjust the light intensity to 1000 μw/cm ² /s by adjusting the knob, and perform the test. During the test, both the negative phototaxis reaction chamber 2 and the habitat chamber 6 were covered with black non-reflective foam paper shading sleeves. Due to the angle of the Y-shaped device design, when the light source was turned on, there was almost no light in the habitat chamber 6. Opening is basically the same; and there is weak light in the negative phototaxis reaction chamber 2.

The black non-reflective foam paper shade was removed after two hours of testing. The number of citrus psyllids in the negative phototaxis reaction chamber 2, the positive phototaxis reaction chamber 5 and the habitat chamber 6 were recorded respectively, with 4 repetitions in total, 20 psyllids each time. Each test was replaced with new test insects.

Positive phototaxis rate = number of positive phototaxis chamber insects/20*100%

Negative phototaxis rate = number of negative phototaxis chamber insects/20*100%

Positive/negative phototaxis index=|(number of positive phototaxis insects - number of negative phototaxis insects)|/20*100%

Total photoresponse index = (number of positive phototaxis chamber insects + number of negative phototaxis chamber insects)/20*100%

The positive phototaxis rate refers to the ratio of the number of phototaxis insects (the number of positive phototaxis chamber insects) to the total number of tested insects, and it represents the total amount of insects moving toward the light source.

The negative phototaxis rate refers to the ratio of the number of negative phototaxis insects (the number of negative phototaxis chamber insects) to the total number of tested insects, and it represents the total amount of insects moving away from the light source.

The positive/negative phototaxis index refers to the ratio of the absolute value of the difference between the number of phototaxis insects and the number of negative phototaxis insects to the total number of tested insects. It is the net change of insects moving toward or away from the light source, reflecting that the insect population is positive phototaxis Whether the behavior is the majority or the negative phototaxis behavior is the majority. If the number of positive phototaxis insects - the number of negative phototaxis insects > 0, the insect species is dominated by positive phototaxis behavior under a certain wavelength or intensity of light. If the positive phototaxis The number of insects - the number of negative phototactic insects < 0, the insect species has a negative phototaxis behavior under a certain wavelength or intensity of light, if the number of positive phototactic insects - the number of negative phototactic insects ≈ 0, the insect species in Under a certain wavelength or intensity of light, both positive and negative phototaxis are close to each other, or there is no response to light.

The total photoresponse index refers to the ratio of the sum of the number of positive phototaxis insects and the number of negative phototaxis insects to the total number of tested insects, which indicates the ability of the light source to affect insect behavior.

3. Experimental results and analysis

The measured test results are shown in Tables 1-4.

Table 1 Positive phototaxis of citrus psyllids under different wavelength light sources

wavelength (nm) Positive phototaxis (%) 365.0±10 71.24±2.39abc 419.9±10 90.00±5.77a 434.7±10 87.50±6.29ab 459.2±10 88.75±2.39a 501.6±10 87.50±7.77ab 610.6±10 61.25±5.54cd 691.5±10 63.75±4.27bc 729.0±10 38.75±4.27d 780.0±10 11.25±5.15e

Note: value = mean ± standard error, F _8,35 = 27.398, P <0.001, the same letter in the table means that the difference is not significant (the analysis method adopts Tukey pair test in One-Way ANOVA of SPSS18.0 software , P < 0.05 was considered significant difference).

Table 2 Negative phototaxis of citrus psyllids under different wavelength light sources

wavelength (nm) Negative phototaxis (%) 365.0±10 2.63±2.63a 419.9±10 0.00±0.00a 434.7±10 0.00±0.00a 459.2±10 2.5±2.5a 501.6±10 0.00±0.00a 610.6±10 0.00±0.00a 691.5±10 0.00±0.00a 729.0±10 1.25±1.25a 780.0±10 0.00±0.00a

Note: value = mean ± standard error, F _8,35 = 0.780, P = 0.624, the same letter in the table means that the difference is not significant (the analysis method uses SPSS18.0 software single factor analysis of variance One-Way ANOVA Tukey pair test , P < 0.05 was considered significant difference).

Table 3 Positive phototaxis index of citrus psyllid under different wavelength light sources

wavelength (nm) Positive phototaxis index (%) 365.0±10 68.75±2.39abc 419.9±10 90.00±5.77a 434.7±10 87.50±6.23ab 459.2±10 86.25±4.27abc 501.6±10 87.50±7.77ab 610.6±10 61.25±5.54cd 691.5±10 63.75±4.27bc 729.0±10 37.50±5.20d 780.0±10 11.25±5.15e

Note: value = mean ± standard error, F _8,35 = 24.914, P <0.001, the same letter in the table means that the difference is not significant (analysis method using SPSS18.0 software single factor analysis of variance One-Way ANOVA Tukey pair test , P < 0.05 was considered significant difference).

Table 4 The total photoresponse index of citrus psyllid under different wavelength light sources

wavelength (nm) Total photoresponse index (%) 365.0±10 73.75±4.27abc 419.9±10 90.00±5.77a 434.7±10 87.50±6.29ab 459.2±10 91.25±2.39a 501.6±10 87.50±7.77ab 610.6±10 61.25±5.54cd 691.5±10 63.75±4.27bcd 729.0±10 40.00±3.53d 780.0±10 11.25±5.15e

Note: value = mean ± standard error, F _8,35 = 26.978, P <0.001, the same letter in the table means that the difference is not significant (the analysis method uses SPSS18.0 software single factor analysis of variance One-Way ANOVA Tukey pair test , P < 0.05 was considered significant difference).

In this experiment, the positive/negative phototaxis behavior responses of citrus psyllid adults to 9 kinds of wavelength light sources were studied, and the results are shown in Tables 1-4. Even though the light source adopted is a single-wavelength light source, the light emitted is still light in a small continuous wavelength range. The wavelength fluctuation range of the light emitted by the single-wavelength light source used in the test of the present invention is 10nm.

Through the analysis of the phototaxis rate, it was found that the intensity of adult citrus psyllids to the wavelengths of 419.9±10nm, 434.7±10nm, 459.2±10nm and 501.6±10nm (that is, the wavelength ranges from 409.9nm to 469.2nm and 491.6nm to 511.6nm) was The light source of 1000 μw/cm ² /s has the strongest tendency. Through the analysis of negative phototaxis rate, it was found that the negative phototaxis behavior of citrus psyllids was weak (Table 2). The light source with the wavelength of 419.9±10nm, 434.7±10nm, 459.2±10nm and 501.6±10nm and the intensity of 1000 μw/cm ² /s had the strongest net attraction ability to citrus psyllid adults (Table 3). The wavelengths of 419.9±10nm, 434.7±10nm, 459.2±10nm and 501.6±10nm and the intensity of 1000 μw/cm ² /s have a greater impact on the total photoreaction behavior of the light source (Table 4), which can also play a role in field control. to a certain avoidance effect.

In summary, when the light source with a wavelength of 409.9nm-511.6nm has a good effect on the lure and control of citrus psyllids, especially the light source with a wavelength of 409.9nm-469.2nm and 491.6nm-511.6nm can be used in the production of field traps application.

In addition, regarding the effect of light intensity, when the light intensity is 300-1200μw/cm ² /s, the light source with the above wavelengths has a good effect of trapping citrus psyllids, and when the light intensity is 300-1000μw/cm ² /s , the trapping effect increases with the increase of light intensity; when the light intensity exceeds 1000μw/cm ² /s, the trapping effect is basically the same as that of 1000μw/cm ² /s, and even the trapping effect has a slight decline. Among them, when the light intensity is 800-1000 μw/cm ² /s, the trapping effect is better, and when the light intensity is 1000 μw/cm ² /s, the trapping effect is the best.

Example 2

The citrus psyllids were trapped with traps whose wavelengths of light sources were 419.9±10nm, 459.2±10nm and 501.6±10nm respectively. The schematic diagram of the structure of the trap is shown in Fig. 2 and Fig. 3 . Wherein, Fig. 2 is a front view, and Fig. 3 is a side view.

The trap uses light of a single wavelength as a light source, wherein the wavelength of the light source of trap 1 is 419.9nm±10nm, the wavelength of light source of trap 2 is 459.2nm±10nm, and the wavelength of light source of trap 3 is 501.6nm±10nm.

The trap includes a light source 18, a hook 11, a solar panel 12, a power supply and a timing device 13, an adhesive yellow plate 17, a waterproof baffle 14, a color plate support 15 and a draw-in slot 16. Wherein, the waterproof baffle 14 is installed on the top of the swatch support 15, and is used to protect the light source 18 (because the power supply and the timing device 13 are arranged inside the waterproof baffle 14, they are not marked in the figure); Timing device power supply; waterproof baffle 14 is arranged on the periphery of light source 18, is used to protect light source 18; Color plate support 15 is provided with draw-in groove 16, is used for blocking sticky yellow plate 17, is convenient to replace new sticky yellow plate; Said power supply is lithium battery, also can be other commonly used batteries.

Test method: Hang traps 1, 2, and 3 at a place 50cm higher than the top of Jiulixiang. Set the turn-on time from 6:00 pm to 6:00 am the next day. After 3 days of trapping, the number of citrus psyllids trapped on the yellow board was recorded, and the yellow board of the same size was used as the control, and each treatment was repeated three times.

The measured results are shown in Figure 4. As can be seen from Figure 4, using the sticky yellow board alone has a certain trapping effect on citrus psyllids, but the effect is not good; while traps 1, 2 and 3 have significantly better trapping effects on citrus psyllids than the sticky yellow board alone, It is more than twice the trapping effect of a single sticky yellow board; the trap 1 with a wavelength of 419.9nm±10nm has the best trapping effect, followed by the trap 2 with a wavelength of 459.2nm±10nm, and the trapping effect of trap 3 is the same as that of trapping 2 has the same effect.

The above results show that the trap combined with the sticky yellow plate and the light source of a specific wavelength has a significantly higher trapping ability on citrus psyllids than the trap with a single color plate, and has good application value.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the scope of the present invention. For those of ordinary skill in the art, on the basis of the above descriptions and ideas, they can also make There is no need to and cannot exhaustively list all the implementation manners for other changes or changes in different forms. All modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included within the protection scope of the claims of the present invention.

Claims (10)

1. The application of a special light source in trapping citrus psyllids, characterized in that the wavelength of the special light source is 409.9nm to 511.6nm. 2. The application of the special light source in trapping citrus psyllids according to claim 1, characterized in that the wavelength of the special light source is 409.9 nm to 469.2 nm or 491.6 nm to 511.6 nm. 3. The application of the special light source in trapping citrus psyllids according to claim 2, characterized in that the wavelength of the special light source is 419.9±10nm, 434.7±10nm, 459.2±10nm or 501.6±10nm. 4. The application of the special light source in trapping citrus psyllids according to claim 3, characterized in that the special light source can be a combination of multiple wavelengths. 5. The application of the special light source according to any one of claims 1 to 4 in trapping citrus psyllids, characterized in that the special light source can be an LED light source or a fluorescent lamp. 6. A trap for citrus psyllid, characterized in that the wavelength of the light source of the trap is 409.9nm-511.6nm. 7 . The trap for citrus psyllids according to claim 6 , wherein the light source of the trap has a wavelength of 409.9 nm to 469.2 nm or 491.6 nm to 511.6 nm. 8. according to the trap of the described citrus psyllid of claim 7, it is characterized in that, described trap comprises light source, power supply and timing device, viscous yellow board and swatch support; Described power supply is used to supply power to light source and timing device ; The light source, power supply, and timing device are installed on the upper end of the swatch support; the viscous yellow board is arranged at the lower end of the swatch support, below the light source. 9. The trap of citrus psyllid according to claim 8, characterized in that, the trap also includes a waterproof baffle; Inside the baffle, the light source is arranged under the waterproof baffle. 10. according to the trap of the described citrus psyllid of claim 8, it is characterized in that, described swatch support is provided with draw-in groove, is used for clipping sticky yellow plate; Described power supply is lithium battery; Described trap also A solar panel is provided; the solar panel is electrically connected with the light source and the timing device, and is installed on the top of the waterproof baffle, and the top of the trap is also provided with a hook.