CN108872520A - A kind of water-quality test method, system and equipment - Google Patents
A kind of water-quality test method, system and equipment Download PDFInfo
- Publication number
- CN108872520A CN108872520A CN201810922505.1A CN201810922505A CN108872520A CN 108872520 A CN108872520 A CN 108872520A CN 201810922505 A CN201810922505 A CN 201810922505A CN 108872520 A CN108872520 A CN 108872520A
- Authority
- CN
- China
- Prior art keywords
- water sample
- water
- sample
- uniform
- toxicity value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of water-quality test method, including：Water sample to be tested is filtered, water after filter sample is obtained；Homogeneous processing is carried out to water sample after the filtering, obtains uniform water sample；Parameter in bio kinetic model is carried out to the uniform water sample, obtains toxicity value.Water sample to be tested using after being filtered by method provided by the invention, the method for carrying out homogeneous processing, and in the prior art by the way of centrifuge separation, so that the suspended components in water sample are centrifuged away, it cannot obtain true aqueous ingredient, homogeneous processing method of the invention, so that the suspended components in water sample are dissolved in or more uniform are distributed in water sample, so that water sample is uniform, it ensure that the authenticity of water sample, water quality can be detected in the case where the primitive component for not introducing interfering substance and guarantee water sample does not lose or is rotten.The present invention also provides a kind of water-quality test system and equipment, it may have above-mentioned beneficial effect, details are not described herein.
Description
Technical field
The present invention relates to water quality inspection technique field, in particular to a kind of water-quality test method, system and equipment.
Background technique
With the increasingly diversification of sewage and waste water, the requirement to stain disease processing is also continuously improved, such as requires control
Toxic pollutant in stain disease.Mainly there are traditional physico-chemical method and biological method to the measurement of stain disease toxicity at present.By
Containing various impurity in stain disease, type is complicated, and water sample is muddy, though it is main in traditional Physico-chemical tests energy quantitative analysis pollutant
Want the content of ingredient, but that there are test items is more, at high cost, is difficult to monitor all kinds of poisonous substances, cannot directly, comprehensively reflect water
The problems such as various pollutants are to the combined influence of environment in sample.And biological test method can make up for it traditional Physico-chemical tests method
Deficiency can effectively detect the comprehensive organism effect of all pollutants altogether in water body, the safety of energy visual evaluation water quality, and grasp
It is few to make simple test number.Therefore in the research of water pollution and control, biological method has become monitoring and evaluation water body
One of the important means of environment.Biological method of the photobacteria toxicity test as toxicity detection, because of its simple, quick, spirit
It is quick and cost is relatively low and it is of increasing concern.
Currently, stain disease biological synthesis toxicity detection mostly uses Fermi operator laboratory biological method.It is used in this method
Fermi operator be Marine Bacteria, to salinity requirements height, need to adjust salinity in test process.And the salt of high concentration may will affect
The biology toxicity of certain toxicants shows, studies have found that the NaCl of high concentration can make to detect some toxicity in photobacteria
The toxicity shown when substance is less than normal.With the bio-toxicity of recombination photogen detection water body have it is simple, quick, to salinity requirements
The features such as low, thus be more and more favored.
Since waste component is complicated, there are some suspended matters or insoluble particles objects in water sample, it is necessary to just by pretreatment
It can be carried out test.But preprocess method cannot make component damages to be measured or rotten, should not introduce interfering substance.Traditional experiment
The water samples preprocessing means such as room filter centrifugation, can remove part test substance, cause test result inaccurate.
Therefore, how a kind of pretreating scheme of waste water is provided, interfering substance can not introduced, and guarantee water sample
In the case that primitive component does not lose or is rotten, water quality is detected, is that those skilled in the art's technology urgently to be resolved is asked
Topic.
Summary of the invention
In view of this, can not introduced the purpose of the present invention is to provide a kind of water-quality test method, system and equipment
Interfering substance, and in the case where guaranteeing that the primitive component of water sample does not lose or is rotten, water quality is detected.Its concrete scheme
It is as follows：
In a first aspect, the present invention provides a kind of water-quality test method, including：
Water sample to be tested is filtered, water after filter sample is obtained；
Homogeneous processing is carried out to water sample after the filtering, obtains uniform water sample；
Parameter in bio kinetic model is carried out to the uniform water sample, obtains toxicity value.
Preferably,
It is described to further include after obtaining toxicity value to the uniform water sample progress parameter in bio kinetic model：
Whether the toxicity value for judging the uniform water sample is more than default toxicity value；
If it is, carrying out the dilution of presupposition multiple, the toxicity of the uniform water sample after dilution to the uniform water sample
Value is no more than default toxicity value.
Preferably,
It is described that homogeneous processing is carried out to water sample after the filtering, including：
The homogeneous processing of ultrasonic wave is carried out to water sample after the filtering.
Preferably,
It is described to further include after obtaining toxicity value to the uniform water sample progress parameter in bio kinetic model：
Using the disturbing factor calibration value before blank control group and the uniform water sample unreacted, to the uniform water sample
It measures luminous intensity in test process to be corrected, and toxicity value obtained by calculation.
Second aspect, the present invention provide a kind of water-quality test system, including：
Water after filter sample is obtained for being filtered to water sample to be tested in water sample filtering module；
The homogeneous module of water sample obtains uniform water sample for carrying out homogeneous processing to water sample after the filtering；
Toxotest module obtains toxicity value for carrying out parameter in bio kinetic model to the uniform water sample.
Preferably, further include：
Toxicity value judgment module, for judging whether the toxicity value of the uniform water sample is more than default toxicity value；
Water sample dilutes module, for if it is determined that the toxicity value of the uniform water sample is more than default toxicity value, then to described
Uniform water sample carries out the dilution of presupposition multiple, and the toxicity value of the uniform water sample after dilution is no more than default toxicity value.
Preferably,
The homogeneous module of water sample, including：
The homogeneous module of ultrasonic wave, for carrying out the homogeneous processing of ultrasonic wave to water sample after the filtering.
Preferably, further include：
Correction module is compareed, for calibrating using the disturbing factor before blank control group and the uniform water sample unreacted
Value, is corrected, and toxicity value is obtained by calculation to luminous intensity is measured in the uniform water sample test process.
The third aspect, the present invention provide a kind of water-quality test equipment, including：
Water after filter sample is obtained for being filtered to water sample to be tested in water sample filter；
Water sample homogenizer obtains uniform water sample for carrying out homogeneous processing to water sample after the filtering；
Water sample tester obtains toxicity value for carrying out parameter in bio kinetic model to the uniform water sample.
Preferably, further include：Rectifier is compareed, before using blank control group and the uniform water sample unreacted
Disturbing factor calibration value is corrected to luminous intensity is measured in the uniform water sample test process, and poison is obtained by calculation
Property value.
The present invention provides a kind of water-quality test method, including：Water sample to be tested is filtered, water after filter sample is obtained；
Homogeneous processing is carried out to water sample after the filtering, obtains uniform water sample；Parameter in bio kinetic model is carried out to the uniform water sample, is obtained
Toxicity value.Water sample to be tested using after being filtered by method provided by the invention, the method for carrying out homogeneous processing, and existing skill
In art by the way of centrifuge separation, so that the suspended components in water sample are centrifuged away, true water sample cannot be obtained
Ingredient, homogeneous processing method of the invention so that the suspended components in water sample dissolve in or it is more uniform be distributed in water sample, make
It is uniform to obtain water sample, ensure that the authenticity of water sample, interfering substance can not introduced, and guarantee that the primitive component of water sample is not damaged
In the case where losing or being rotten, water quality is detected.
The present invention also provides a kind of water-quality test system and equipment, it may have above-mentioned beneficial effect, details are not described herein.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis
The attached drawing of offer obtains other attached drawings.
Fig. 1 is a kind of flow chart of water-quality test method provided by a kind of specific embodiment of the present invention；
Fig. 2 is a kind of flow chart of water-quality test method provided by another specific embodiment of the invention；
Fig. 3 is a kind of composition schematic diagram of water-quality test system provided by another specific embodiment of the invention；
Fig. 4 is a kind of expansion composition signal of water-quality test system provided by another specific embodiment of the invention
Figure；
Fig. 5 is a kind of structural schematic diagram of water-quality test equipment provided by a kind of specific embodiment of the present invention.
Appended drawing reference is as follows：
1.PLC terminal control mechanism, 2. Dual channel detection devices, calibration module, 3. reagents draw module, 4. strain low temperature
Save pond, 5. strain adaptation pools, 6. reaction tanks (including blank sample reaction tank and water sample reaction tank), 7. peristaltic pumps, 8. pure
Bucket, 9. water sample preprocessors, 10.KCl solution tank, 11. waste liquid barrels, the homogeneous processing module of 12. water samples, 13. purifying water pools,
14. waste discharge liquid pool, 15 water samples save pond, 16.KCl solution pool, 17. temperature control modules, 18. frosted glass plates, 19. detection pipes,
20. photomultiplier tube.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to FIG. 1, Fig. 1 is a kind of process of water-quality test method provided by a kind of specific embodiment of the present invention
Figure.
In a kind of specific embodiment of the invention, the embodiment of the present invention provides a kind of water-quality test method, including：
S11：Water sample to be tested is filtered, water after filter sample is obtained；
In embodiments of the present invention, the present invention is filtered water sample to be tested, is obtained after obtaining water sample to be tested
Water sample after filter.Specifically, it can use diaphragm pump and extract water sample, dispose 200 mesh fore filters in water pipe entrance, filter off
Sundries and floating material.The fore filter resistance to acid and alkali, corrosion resistance is good, and resistance is low, can be cleaned repeatedly, warp
Ji property is comparatively very high, and filter connector is quick coupling, replacement operation very simple.
S12：Homogeneous processing is carried out to water sample after the filtering, obtains uniform water sample；
After being filtered, there is also the insoluble substance such as particulate matter, suspended matter, these substances in water sample after filtering
It is a part of water sample.Therefore, filtered water sample is made to enter the homogeneous module of ultrasonic wave, this module is equipped with sink.Bottom of gullet
2 pieces of shake heads are installed, sink is equipped with water sample sample feeding pipe, water sample outlet pipe, pure water water inlet pipe, drain pipe.Water reaches in module
When to 1/3 or 2/3, closes pump and open shake head and shake 20min, it is molten to increase particulate matter, suspended matter and indissoluble solution substance in water sample
Xie Du keeps water sample uniform.
That is, can be carried out using the short grained mode of ultrasonic grind to water sample after filtering in the present embodiment homogeneous
Processing carries out the homogeneous processing of ultrasonic wave to water sample after the filtering.It is of course also possible to use other homogeneous methods are to water sample
It is handled.
S13：Parameter in bio kinetic model is carried out to the uniform water sample, obtains toxicity value.
Parameter in bio kinetic model is carried out to uniform water sample, obtains toxicity value.If the toxicity value is more than default toxicity value,
The uniform water sample can be diluted, can be diluted
Water sample dilution can reduce sample colorimetry-turbidity, improve indissoluble Solubility of Substances.Pump pure water enters the homogeneous mould of ultrasonic wave
In block, after being mixed with certain proportion with original water sample, opens shake head and shake 20min.Dilution ratio is automatically controlled by instrument, and 1:
It is optional in 10000 ranges.
Specifically, it can default in water sample test process and test original water sample, if test Toxicity of Water Samples value>90%,
That is setting 90% for default toxicity value.Water sample is diluted step by step；If 20%<Toxicity value<90%, test terminates.For example,
It can be arranged such that：If test Toxicity of Water Samples value is：10000 times of dilution ratios can be set in 99%-100%；If test
Toxicity of Water Samples value is：1000 times of dilution ratios can be set in 98%-99%；If test Toxicity of Water Samples value is：97%-98%,
500 times of dilution ratios can be set；If test Toxicity of Water Samples value is：200 times of dilution ratios can be set in 96%-97%；Such as
Fruit tests Toxicity of Water Samples value：100 times of dilution ratios can be set in 95%-96%；If test Toxicity of Water Samples value is：94%-
95%, 50 times of dilution ratios can be set；If test Toxicity of Water Samples value is：10 times of thinner ratios can be set in 93%-94%
Example；If test Toxicity of Water Samples value is：5 times of dilution ratios can be set in 92%-93%；If test Toxicity of Water Samples value is：
2 times of dilution ratios can be set in 90%-92%.
Further, when carrying out parameter in bio kinetic model to water sample, recombination photobacteria can be selected as test receptor
Water sample is tested.Recombination photobacteria can produce in this way：Marine Luminous Bacteria genetic fragment is intercepted, large intestine bar is implemented in
Obtain recombination bacillus coli in bacteria plasmid, bioluminescence gene high efficient expression in recombination bacillus coli, during Metabolism of E. coli,
It is produced from luminescence phenomenon, and improves luminous efficiency.Aqueous bio toxicity is monitored using recombination photogen, does not need high salinity ring
Border avoids high salinity from impacting toxicants certain in water test, easy to operate, reliable.
Referring to FIG. 2, Fig. 2 is a kind of process of water-quality test method provided by another specific embodiment of the invention
Figure.
On the basis of above-mentioned specific embodiment, in present embodiment, in order to correct external environment, do in water sample
The influence for disturbing factor, using the disturbing factor calibration value before blank control group and the uniform water sample unreacted, to described equal
Luminous intensity is measured in even water sample test process to be corrected, and toxicity value is obtained by calculation.
Specifically, Toxicity of Water Samples testing process is as follows：
Recombination photogen is stored in low-temperature storage module (2-4 DEG C), and reaction module contains adaptation pool and reaction tank, reaction tank
Including sample cell and blank sample pond, reaction module temperature constant (13-18 DEG C) and adjustable.Using automatic sucking device by bacterium
Liquid is drawn to adaptation pool from low-temperature storage module and carries out bacterium solution dilution and activity recovery, and the thallus recovery time is 5-15min, every
1min sensor acquires 1 data, naturally it is also possible to using other time intervals, such as 30s, 45s etc., and monitoring thallus recovery
Situation.After the photogen laundering period, while water sample and blank solution being drawn to sample cell and blank sample pond, is surveyed first
The disturbing factor calibration value T of water sample when examination water sample is not yet mixed with photogen, then draws the bacterium solution after recovery simultaneously to sample
Product pond and blank sample pond mix photogen with test water sample and blank sample.Water sample and thallus reaction time 5-30min can
Choosing.During water sample detection, water sample Poisoning substance has an impact photogen activity, and photogen luminous intensity is caused to generate change
Change, acquires 1 data every 1min sensor.All data form reaction process song with time (X-axis)-luminous intensity (Y-axis)
Line.
Wherein, it needs to be corrected luminous intensity in test process, method is as follows：
(1) correction about disturbing factor in water sample
Toxicity Monitoring instrument using photomultiplier tube to photogen during the test luminous intensity (physiological activity) change into
Row monitoring, water sample turbidity and coloration can test photogen luminous intensity signal and generate interference, need to test water sample not yet with shine
Disturbing factor calibration value T when bacterium mixes.After water sample is mixed with photogen in test process, after reading real-time luminous intensity every time
Light intensity is calibrated using T value immediately, deducts background influence caused by water sample turbidity and coloration etc..That is, utilizing
Disturbing factor calibration value T when uniform water sample is not yet mixed with photogen is as calibration value, during the test, the hair read
Light intensity value needs to be corrected with calibration value, is only true luminous value.Because waste water may be to have colo(u)r atlas or muddy sample, in advance
Turbidity or coloration are not reduced in treatment process for holding water sample authenticity.Instrument detection module is optical module, turbidity, color
Degree can produce a very large impact test, it is therefore necessary to be calibrated.
Inside detection module：Equipped with light source, frosted glass plate is housed before light source, it is light emitted for divergent light source, make it
Scattering state is presented.Light scattering is scattered on the colorimetric cylinder equipped with blank and water sample, reaches detector below by colorimetric cylinder.Inspection
It surveys device and detects remaining light intensity value, blank water sample detection value is Tc, and water sample test value is Ts, then the disturbing factor calibration value in water sample
For：T=Ts/Tc.T value tests luminous intensity for calibration sample.After calibration value is completed, close light source, mixed sample and
Photogen monitors and corrects entire test process luminous intensity and variation after photogen mixes with water sample, and each aqueous ingredient
Difference, test all needs to be corrected every time.
That is, using the disturbing factor calibration value before the uniform water sample unreacted, it is right in carrying out test process
It measures luminous intensity to be corrected, eliminates the influence of disturbing factor in water sample.
(2) about the correction of environmental impact factor
Since outside environmental elements such as temperature or photogen activated state can have an impact test result, using double
Air conduct measurement method is simultaneously monitored water sample and blank with photogen reagentia process.Using blank sample to test water sample
Test result is corrected, that is to say, that blank control group is utilized, to measuring luminous intensity in the uniform water sample test process
It is corrected, and the toxicity value being obtained by calculation after correction deducts influence caused by other environmental factors.
According to the luminous intensity of measurement, correction factor f is calculated using formula (1)kt.The factor is for correcting the initial of water sample
Luminous intensity I0(laundering period terminates, and photogen mixes preceding luminous intensity with water sample), as consideration photobacteria itself
The initial luminescence of variations in light.
fkt=Ikt/Ik0(t=5min, 15min, 30min, similarly hereinafter) (1)
Wherein：fktIt is the correction factor for being respectively 5min, 15min or 30min the reaction time；
IktIt is the termination luminous intensity of blank control；
Ik0It is the initial luminescence of blank control (laundering period terminates, and photogen mixes preceding luminous intensity with blank).
3. correction calculation method is as follows：
Calculate water sample initial luminescence I0Corrected value Ict：
Ict=I0·fkt (2)
The relative luminosity T of test water sample is calculated using formula (3)tAnd formula (4) calculates Toxicity of Water Samples value Ht：
Wherein：ItIt is the termination luminous intensity of sample；
T is the disturbing factor calibration value of water sample luminous intensity.
It, can Hg built in online comprehensive toxicity instrument when specific progress Toxicity of Water Samples evaluation2+Concentration-inhibiting rate curve, according to
The inhibiting rate size of tested water sample, calculates considerable amount of Hg automatically2+Concentration.The concentration is obtained multiplied by water sample extension rate
The considerable amount of Hg of noxious material in raw water sample2+Concentration.And then according to Hg2+Concentration judges toxicity size.
The present invention provides a kind of water-quality test method, including：Water sample to be tested is filtered, water after filter sample is obtained；
Homogeneous processing is carried out to water sample after the filtering, obtains uniform water sample；Parameter in bio kinetic model is carried out to the uniform water sample, is obtained
Toxicity value.Water sample to be tested using after being filtered by method provided by the invention, the method for carrying out homogeneous processing, and existing skill
In art by the way of centrifuge separation, so that the suspended components in water sample are centrifuged away, true water sample cannot be obtained
Ingredient, homogeneous processing method of the invention, so that the suspended components in water sample are dissolved in or are evenly distributed in water sample, so that water
Sample is uniform, ensure that the authenticity of water sample, can not introduce interfering substance, and guarantee the primitive component of water sample do not lose or
In the case where rotten, water quality is detected.Using coarse strainer coarse filtration, ultrasonic wave is homogeneous and the modes such as dilution to water sample into
The online pretreatment of row, guarantees the authenticity of water sample.Test result is corrected using modes such as background deduction, blank deductions,
Guarantee the reliability of test result.Provide a kind of fast and accurately wastewater biological toxotest method.
Fig. 3, Fig. 4 are please referred to, Fig. 3 is a kind of water-quality test system provided by another specific embodiment of the invention
Composition schematic diagram；Fig. 4 shows for a kind of expansion composition of water-quality test system provided by another specific embodiment of the invention
It is intended to.
In another specific embodiment of the invention, the embodiment of the present invention provides a kind of water-quality test system 200, packet
It includes：
Water after filter sample is obtained for being filtered to water sample to be tested in water sample filtering module 210；
The homogeneous module 220 of water sample obtains uniform water sample for carrying out homogeneous processing to water sample after the filtering；
Toxotest module 230 obtains toxicity value for carrying out parameter in bio kinetic model to the uniform water sample.
Preferably, water-quality test system 200 further includes：
Toxicity value judgment module 240, for judging whether the toxicity value of the uniform water sample is more than default toxicity value；
Water sample dilutes module 250, for if it is determined that the toxicity value of the uniform water sample is more than default toxicity value, then to institute
The dilution that uniform water sample carries out presupposition multiple is stated, the toxicity value of the uniform water sample after dilution is no more than default toxicity value.
Preferably,
The homogeneous module 220 of water sample, including：
The homogeneous module of ultrasonic wave, for carrying out the homogeneous processing of ultrasonic wave to water sample after the filtering.
Preferably, water-quality test system 200 further includes：
Correction module is compareed, for calibrating using the disturbing factor before blank control group and the uniform water sample unreacted
Value, is corrected, and toxicity value is obtained by calculation to luminous intensity is measured in the uniform water sample test process.
Referring to FIG. 5, Fig. 5 shows for a kind of structure of water-quality test equipment provided by a kind of specific embodiment of the present invention
It is intended to.
In a kind of specific embodiment of the invention, the present invention provides a kind of water-quality test equipment, including：
Water after filter sample is obtained for being filtered to water sample to be tested in water sample filter 410；
Water sample homogenizer 420 obtains uniform water sample for carrying out homogeneous processing to water sample after the filtering；
Water sample tester 430 obtains toxicity value for carrying out parameter in bio kinetic model to the uniform water sample.
Preferably, further include：Rectifier 440 is compareed, for utilizing blank control group and the uniform water sample unreacted
Preceding disturbing factor calibration value is corrected to luminous intensity is measured in the uniform water sample test process, and by calculating
To toxicity value.
Specifically, the water-quality test equipment may include the online pretreatment system of water sample, water sample detection system, temperature control
System processed, data processing system.By the online pretreatment system of control system (1.PLC terminal control mechanism) control water sample, water sample
Detection system and data processing system.
(1) the online pretreatment system of water sample, including：For water sample coarse filtration 9. water sample preprocessors, for water sample it is even
The homogeneous processing module of 12. water samples of matter processing is used for diluted 13. purifying water pool of water sample.
Wherein 9. water sample preprocessors can be set to the quick access device of 200 mesh of aperture, 9. water sample preprocessors with
Pump is connected, and is pumped water sample to the homogeneous processing module of 12. water samples by pumping, even by the way that whether valve control water sample enters 12. water samples
Matter processing module.The homogeneous processing module bottom sides of 12. water sample are equipped with pure water pipe and waste discharge liquid pipe, pass through pump and valve control
Whether pure water processed enters the homogeneous processing module of 12. water samples and whether waste liquid is discharged.The module bottom side is discharged equipped with water sample
Whether pipe enters 6. reaction tanks by water sample after pump and valve control processing.
12. the homogeneous processing module of water sample can be set to closed metal cavity, bottom is equipped with 2 pieces of ultrasonic wave transmitting shakes
Head emits for ultrasonic wave.Water sample and pure water volume are controlled by control water sample and pure penstock, revolution speed and time
Ratio dilutes water sample to realize.
(2) water sample detection system may include：2. Dual channel detection device, calibration module, 3. reagents draw module, 4.
It is strain cryo-conservation pond, 5. strain adaptation pools, 6. reaction tanks (including blank sample reaction tank and water sample reaction tank), 13. pure
Pond, 14. waste discharge liquid pools, 15 water samples save pond, 16.KCl solution pool, interference effect factor correction module in water sample.
By pump, valve control will treated that water sample draws saves pond to 15 water samples and save.By pumping respectively by KCl
Solution and pure water are drawn to 16.KCl solution pool and 13. purifying water pools.
It is robot arm device that reagent, which draws module, controls mechanical arm by positioning device, and mechanical arm is reached and is appointed
What reagent sites.Mechanical arm is equipped with two sampling needle tubings, draws various water samples and reagent by needle tubing.The needle tubing and detection system
Colorimetric cylinder is connected in system.Various reagents and bacterium solution are drawn to adaptation pool and reaction tank by mechanical arm, in reaction process, needle tubing is every
Reaction solution is drawn every 1min, and luminous intensity detection is carried out into colorimetric cylinder.To realize luminous intensity variations in entire reaction process
Monitoring.
2. Dual channel detection device：In monitoring process, blank reaction tank and the every other factor of water sample reaction tank are controlled, is protected
Card other all factor consistency in addition to water sample, and synchronous realization water sample and blank control monitoring simultaneously.
Interference effect factor correction module in water sample：Inside detection system, it is equipped with and corrects light source used, frosted glass plate, if
There are detection colorimetric cylinder, photomultiplier tube used, all components are all 2 sets, for realizing shadow is interfered in Dual channel detection and water sample
Ring factor correction.
(3) 17. temperature control modules may include：It is photogen low temperature memory module, adaptation pool temperature control modules, anti-
Answer pond temperature control modules, housing temperature control module.
Luminous bacterium solution is stored in strain cryo-conservation pond, and cryo-conservation bottom of pond portion is equipped with temperature control modules and temperature detection
Device is configured and is calibrated to bacterium pond temperature by control system.Strain adaptation pool and water sample reaction bottom of pond portion are equipped with temperature control
Molding block and temperature detector are configured and are calibrated to adaptation pool and reaction tank temperature by control system.It is set on the outside of cabinet
Have minitype air conditioner, air conditioning air outlet is communicated with Toxicity Monitoring instrument cabinet, and cabinet inside is equipped with temperature detector, by control system and
Airconditioning control cabinet inside temperature.
(4) data processing system includes：Reading data, data processing, data are shown and the functional modules such as curve is shown.
Specifically, it can be set to 1.PLC terminal control mechanism, by the PLC terminal being located in 1.PLC terminal control mechanism
Control software is read out test data, handles and shows.Reading data includes modules reading data, including each module
Temperature data, detection system test data, various reagents data on stock etc..Data processing and display include the data of modules
Processing and display.PLC terminal control software is human-computer interaction interface, carries out various instructions and operation by the software.
The water-quality test equipment can be with water sample preprocessing module cleaning module, the part that water sample preprocessing module need to clean
Predominantly ultrasonic wave module and water sample filtering module.After test, extra water sample is discharged from drain pipe, is opened after being pumped into pure water
Ultrasonic wave shake head is opened, is closed after 5min and water is discharged from drain pipe, ultrasonic cleaning is carried out to sonication module, repeat to grasp
Make 2-3 times, naturally it is also possible to be other number of operations, be advisable so that clean cleaning can be carried out to modules.Sampling pipe
Route water sample rinse.Primary filter is taken out cleaning or is directly replaced in instrument periodic maintenance.
Finally, it is to be noted that, herein, relational terms such as first and second and the like be used merely to by
One entity or operation are distinguished with another entity or operation, without necessarily requiring or implying these entities or operation
Between there are any actual relationship or orders.Moreover, the terms "include", "comprise" or its any other variant meaning
Covering non-exclusive inclusion, so that the process, method, article or equipment for including a series of elements not only includes that
A little elements, but also including other elements that are not explicitly listed, or further include for this process, method, article or
The intrinsic element of equipment.In the absence of more restrictions, the element limited by sentence "including a ...", is not arranged
Except there is also other identical elements in the process, method, article or apparatus that includes the element.
A kind of water-quality test method provided by the present invention, system and equipment are described in detail above, herein
Apply that a specific example illustrates the principle and implementation of the invention, the explanation of above example is only intended to help
Understand method and its core concept of the invention；At the same time, for those skilled in the art, according to the thought of the present invention,
There will be changes in the specific implementation manner and application range, in conclusion the content of the present specification should not be construed as to this
The limitation of invention.
Claims (10)
1. a kind of water-quality test method, which is characterized in that including：
Water sample to be tested is filtered, water after filter sample is obtained；
Homogeneous processing is carried out to water sample after the filtering, obtains uniform water sample；
Parameter in bio kinetic model is carried out to the uniform water sample, obtains toxicity value.
2. water-quality test method according to claim 1, which is characterized in that
It is described to further include after obtaining toxicity value to the uniform water sample progress parameter in bio kinetic model：
Whether the toxicity value for judging the uniform water sample is more than default toxicity value；
If it is, carrying out the dilution of presupposition multiple to the uniform water sample, the toxicity value of the uniform water sample after dilution is not
More than default toxicity value.
3. water-quality test method according to claim 1, which is characterized in that
It is described that homogeneous processing is carried out to water sample after the filtering, including：
The homogeneous processing of ultrasonic wave is carried out to water sample after the filtering.
4. water-quality test method according to any one of claims 1 to 3, which is characterized in that
It is described that parameter in bio kinetic model is carried out to the uniform water sample, further include：
Using the disturbing factor calibration value before blank control group and the uniform water sample unreacted, the uniform water sample is tested
Luminous intensity is measured in the process to be corrected, and toxicity value is obtained by calculation.
5. a kind of water-quality test system, which is characterized in that including：
Water after filter sample is obtained for being filtered to water sample to be tested in water sample filtering module；
The homogeneous module of water sample obtains uniform water sample for carrying out homogeneous processing to water sample after the filtering；
Toxotest module obtains toxicity value for carrying out parameter in bio kinetic model to the uniform water sample.
6. water-quality test system according to claim 5, which is characterized in that further include：
Toxicity value judgment module, for judging whether the toxicity value of the uniform water sample is more than default toxicity value；
Water sample dilutes module, for if it is determined that the toxicity value of the uniform water sample is more than default toxicity value, then to described uniform
Water sample carries out the dilution of presupposition multiple, and the toxicity value of the uniform water sample after dilution is no more than default toxicity value.
7. water-quality test system according to claim 5, which is characterized in that
The homogeneous module of water sample, including：
The homogeneous module of ultrasonic wave, for carrying out the homogeneous processing of ultrasonic wave to water sample after the filtering.
8. according to the described in any item water-quality test systems of claim 5 to 7, which is characterized in that further include：
Correction module is compareed, for using the disturbing factor calibration value before blank control group and the uniform water sample unreacted,
It is corrected to luminous intensity is measured in the uniform water sample test process, and toxicity value is obtained by calculation.
9. a kind of water-quality test equipment, which is characterized in that including：
Water after filter sample is obtained for being filtered to water sample to be tested in water sample filter；
Water sample homogenizer obtains uniform water sample for carrying out homogeneous processing to water sample after the filtering；
Water sample tester obtains toxicity value for carrying out parameter in bio kinetic model to the uniform water sample.
10. water-quality test equipment according to claim 9, which is characterized in that further include：Rectifier is compareed, for utilizing
Disturbing factor calibration value before blank control group and the uniform water sample unreacted, to being surveyed in the uniform water sample test process
It obtains luminous intensity to be corrected, and toxicity value is obtained by calculation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810922505.1A CN108872520A (en) | 2018-08-14 | 2018-08-14 | A kind of water-quality test method, system and equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810922505.1A CN108872520A (en) | 2018-08-14 | 2018-08-14 | A kind of water-quality test method, system and equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108872520A true CN108872520A (en) | 2018-11-23 |
Family
ID=64318042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810922505.1A Pending CN108872520A (en) | 2018-08-14 | 2018-08-14 | A kind of water-quality test method, system and equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108872520A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975228A (en) * | 2019-05-10 | 2019-07-05 | 苏州华能检测技术有限公司 | A kind of wastewater toxicity detection method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560491A (en) * | 2008-04-15 | 2009-10-21 | 中国科学院上海生命科学研究院 | Luminous bacteria and application thereof in detecting general biological toxicity in food or water sample |
| CN102495188A (en) * | 2011-12-09 | 2012-06-13 | 中国科学院烟台海岸带研究所 | Online automatic dynamic exposure and water body treatment system in laboratory |
| CN103045713A (en) * | 2013-01-18 | 2013-04-17 | 张帆 | Method for detecting trace amounts of microbes in a liquid sample containing multiple impurities |
| CN104459074A (en) * | 2014-12-19 | 2015-03-25 | 西安建筑科技大学 | Experimental method for measuring water body eutrophication driving factor |
| CN107478795A (en) * | 2017-08-24 | 2017-12-15 | 山东省城市供排水水质监测中心 | A kind of detection method of urban drinking water water body genetoxic |
| CN108226430A (en) * | 2017-12-28 | 2018-06-29 | 广州华清环境监测有限公司 | A kind of monitoring water quality on line preprocess method |
-
2018
- 2018-08-14 CN CN201810922505.1A patent/CN108872520A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560491A (en) * | 2008-04-15 | 2009-10-21 | 中国科学院上海生命科学研究院 | Luminous bacteria and application thereof in detecting general biological toxicity in food or water sample |
| CN102495188A (en) * | 2011-12-09 | 2012-06-13 | 中国科学院烟台海岸带研究所 | Online automatic dynamic exposure and water body treatment system in laboratory |
| CN103045713A (en) * | 2013-01-18 | 2013-04-17 | 张帆 | Method for detecting trace amounts of microbes in a liquid sample containing multiple impurities |
| CN104459074A (en) * | 2014-12-19 | 2015-03-25 | 西安建筑科技大学 | Experimental method for measuring water body eutrophication driving factor |
| CN107478795A (en) * | 2017-08-24 | 2017-12-15 | 山东省城市供排水水质监测中心 | A kind of detection method of urban drinking water water body genetoxic |
| CN108226430A (en) * | 2017-12-28 | 2018-06-29 | 广州华清环境监测有限公司 | A kind of monitoring water quality on line preprocess method |
Non-Patent Citations (1)
| Title |
|---|
| 蒋浩: "SBR去除保险粉废水COD的研究", 《中国优秀硕士论文全文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975228A (en) * | 2019-05-10 | 2019-07-05 | 苏州华能检测技术有限公司 | A kind of wastewater toxicity detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106769938A (en) | A kind of total nitrogen in-line analyzer and its detection method | |
| US20130149790A1 (en) | Sample preparation system for an analytical system for determining a measured variable of a liquid sample | |
| CN102980983A (en) | Online monitoring analyzer and monitoring system for sewage biotoxicity | |
| CN104849422A (en) | Ammonia nitrogen on-line monitoring system and method thereof | |
| CN104007277B (en) | A kind of bio-toxicity automonitor and monitoring method | |
| CN107966406A (en) | A kind of water quality automatic checkout equipment | |
| CN108459146A (en) | Assess method of the waste water to water treatment micro-organism toxicity | |
| CN106053751A (en) | Water quality monitoring system | |
| CN205352945U (en) | Agricultural product drug residue synthesizes toxicity on -line measuring device | |
| CN110186970A (en) | A kind of sewage treatment plant inflow toxicity on-line measuring device | |
| CN102393392A (en) | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof | |
| CN109856121A (en) | A kind of device of water quality biological toxicity on-line checking | |
| CN114778491A (en) | Heavy metal water quality testing device | |
| CN108872520A (en) | A kind of water-quality test method, system and equipment | |
| CN105277535A (en) | On-site rapid detection method for ammonia nitrogen in water and capable of eliminating reagent blank effects | |
| CN112147202B (en) | Electrochemical-based soil nitrate nitrogen real-time detection system and method | |
| CN102183910A (en) | Method for detecting specific oxygen utilization rate of activated sludge microorganism online based on frequency control | |
| CN207623217U (en) | A kind of water quality automatic checkout equipment | |
| CN205027662U (en) | Scope antiseptic solution performance detecting system | |
| CN208224234U (en) | A kind of water pollution source on-line monitoring device | |
| CN214150541U (en) | Soil nitrate nitrogen real-time detection system based on electrochemistry | |
| CN204924924U (en) | Online cyanide water quality analyzer | |
| CN206337049U (en) | A kind of on-line automatic processing system of spunlace non-woven cloth process water | |
| CN111060157A (en) | Miniature surface water environmental quality check out test set | |
| CN111220602A (en) | Quick detection device of carbamate pesticide residue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181123 |
|
| RJ01 | Rejection of invention patent application after publication |