CN108459146A - Assess method of the waste water to water treatment micro-organism toxicity - Google Patents
Assess method of the waste water to water treatment micro-organism toxicity Download PDFInfo
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Abstract
The present invention discloses a kind of method for assessing waste water to water treatment micro-organism toxicity.The method includes the following steps：1) water treatment micro-organism test bacterium solution is prepared；2) by more parts of wastewater dilution to be measured, every part of extension rate is different；3) waste water after dilution is added to mixing in the water treatment micro-organism test bacterium solution prepared；4) the microorganism increment in every part of mixed solution after mixing is timed in the given time or the real time measure is to obtain every part of mixed solution microbial growth curve；5) microbial growth curve will be measured to be compared with blank control.The method of the present invention has universality, evaluable to the bio-toxicity of different types of waste water, and the testing result of the bio-toxicity by separate sources, the waste water of different extension rates, and waste water dual treatment and (reinforcing) can be instructed to pre-process.
Description
Technical field
The invention belongs to field of water pollution control, more particularly to waste water fits the evaluation method of water treatment micro-organism toxicity
For industrial wastewater toxicity evaluation and waste water dual treatment or strenuous primary treatment.
Background technology
The processing and zero-emission of industrial wastewater are the problems that sewage disposal circle both at home and abroad is generally acknowledged at present.Sewage treatment process
Mostly with biological treatment for main body technology, and the effluent part that enterprise or industrial park treating stations are discharged into contains hazardous contaminant
Often there is toxicity to microorganism, generate inhibition or lethal, cause biological treatment effect bad.Industrial wastewater complicated component, if
The type and concentration of pollutant in waste water are analyzed and are detected, then studies the toxicity properties of each substance, workload and
It is costly, it is time-consuming longer, and cannot intuitively provide influence of the waste water to biological treatment system.Liu Rui in 2011 et al. pairs
The bio-toxicity of the waste discharge of each workshop section of certain pharmacy corporation is evaluated, and finds effluent part after Aerobic biological process not only
Total organic carbon is not reduced, and toxicity rises, and inhibiting effect is generated to sludge, it is therefore proposed that this workshop section's waste water cannot directly enter
Oxygen biological treatment system.Beautiful the dropping studies have shown that working as waste strength to Chinese medicine processing waste water Analysis on Pollution Characteristics of Wang Jun in 2015
Down to influent density 12.5% when, bio-toxicity is reduced to less toxic level substantially.
In view of the above-mentioned problems, a series of detection method of wastewater biological toxicity occurs.For example, application No. is
The patent of invention of 201611171167.X discloses a kind of method for testing oil extraction waste water bio-toxicity using Vibrio-qinghaiensis sp. Q67.
This method is test waste water with oil extraction waste water, is instruction biology with Vibrio-qinghaiensis sp. Q67, using 96 microwell plates as carrier, using micropore
Plate spectrophotometer detects its luminous intensity.By calculating the luminous inhibiting rate of photogen, to judge the comprehensive of oil extraction waste water
Toxicity size is closed, solves the problems, such as that conventional physical and chemical index cannot indicate oil extraction waste water bio-toxicity.Such method, use are specific
Instruction biology, cannot directly show toxic degree of the waste water to water treatment micro-organism, and be mostly used to evaluate the risk of discharge of wastewater
Property.For another example, application No. is 201210499187.5 patents of invention to disclose a kind of sewage life based on system dissolved oxygen concentration
Object toxicity assay, includes the following steps：S1. activated sludge is added in reactor and cleans and configure to setting through clear water
Sludge concentration and system volume；S2. reactor and activity is added through pre-exposure gas disposal Later Zhou Dynasty, one of the Five Dynasties phase property in sewage treatment plant's actual sewage
Sludge system is reacted；S3. the acquisition of DO sensors obtains the system DO indicatrixes in periodic regularity variation in reactor.It should
Analytical technology of the invention based on activated sludge reaction system dissolved oxygen (DO) variation characteristic is developed, and the variation of system DO will be in
Reveal from normal sewage into fashionable different trend feature, this variation tendency is the intuitive table that activity of activated sludge is suppressed
Existing, acquisition acquisition DO characteristics carry out calculating analysis by customized sludge activity inhibiting rate formula in the case of two kinds, can
Rapidly and accurately identify and assess the bio-toxicity degree of sewage.The method can evaluate biology poison of the waste water to activated sludge
Property, but in the presence of time-consuming, the requirement to preaeration degree, sludge concentration is higher.
Invention content
To overcome drawbacks described above, the object of the present invention is to provide a kind of assessment waste water to the side of water treatment micro-organism toxicity
Method, this method can be measured the microbiological comprehensive toxicity of poisonous and harmful substance in waste water, easy to operate, judge waste water pair
The toxicity of biological treatment section microorganism is strong and weak, visual result.
In order to achieve the above objectives, for waste water of the present invention to the method for water treatment micro-organism toxicity, the method includes following
Step：
1) water treatment micro-organism test bacterium solution is prepared；
2) by more parts of wastewater dilution to be measured, every part of extension rate is different；
3) waste water after dilution is added to mixing in the water treatment micro-organism test bacterium solution prepared；
4) the microorganism increment in every part of mixed solution after mixing is timed in the given time or is surveyed in real time
Determine to obtain every part of mixed solution microbial growth curve；
5) microbial growth curve will be measured to be compared with blank control.
Preferably, absorbance of the test bacterium solution at wavelength 450-650nm reaches 0.3-0.7.
Preferably, the water treatment micro-organism test bacterium solution is prepared according to the following steps：
11) activated sludge for acquiring Aerobic biological process section, fully breaks up mixing；
12) activated sludge for breaing up mixing carries out step dilution；
13) sludge seeding after diluting cultivates 16-18h on tablet in 20-35 DEG C of constant incubator；
14) microorganism of the clump count on one group of tablet of 30-300 rear mixing under sterile washing is chosen, TSB is inoculated in
In culture medium.
Preferably, the water treatment micro-organism test bacterium solution can also be prepared according to the following steps, especially active dirty
In the case that mud cannot acquire acquisition in time, can water treatment micro-organism be subjected to freezen protective, before testing,
11) active sludge microorganism for taking out freezen protective, melts in 4 DEG C of refrigerators, is added in TSB culture mediums, is put into
Shaken cultivation is brought back to life in 20 DEG C of shaken cultivation casees；
12) bacterium solution after resurrection is inoculated on tablet, 16-18h is cultivated in 20 DEG C of constant incubators；
13) microorganism of the clump count on one group of tablet of 30-300 rear mixing under sterile washing is chosen, TSB is inoculated in
In culture medium, absorbance of the test bacterium solution at wavelength 450-650nm is obtained between 0.3-0.7.
Preferably, the waste water water sample is to adopt that phosphate buffer solution is diluted, the pH of the phosphate buffer solution
Value is 7-8.
Preferably, the blank control uses artificial wastewater, the artificial wastewater to be made of following raw materials：Glucose
150mg/L；Soluble starch 100mg/L；Sodium carboxymethylcellulose 50mg/L；Tryptone 100mg/L；Ammonium sulfate 126mg/L；
Potassium dihydrogen phosphate 31mg/L；Sodium bicarbonate 250mg/L, with water dissolution constant volume.
Preferably, the measurement of the microorganism increment is using turbidimetric assay, step is：
Every part of mixed solution, negative control, positive control after mixing is added in cuvette；
Each cuvette is put into together warmed-up and remains 35 DEG C of microorganism than in opacimetry instrument, being carried out at the same time than turbid
It measures；
Wherein, location parameter is set：35 DEG C of cultivation temperature, shakes interval time 5min, and single shakes time 20s, shaking
Frequency is 0.2HZ, monitoring time started 40min (monitoring of 40min progress absorbance after starting experiment), when monitoring is spaced
Between 10min；Total experimental period 3-6h；Monitoring wavelength is 450-650nm.
It will measure microbial growth curve the step of being compared with blank control preferably, described and be：
When comparing positive control and entering stationary phase t1, whether wastewater sample also enters stationary phase：
If wastewater sample has been enter into stationary phase, the absorbance value A of wastewater sample when comparing t1WiWith the extinction of positive control
Angle value APIf AWi/AP=1 ± 0.1, then it is regarded as no biotoxicity；
If wastewater sample is not also to stationary phase, the absorbance value A of wastewater sample when comparing monitoring time t2WiIt is right with the positive
According to absorbance value APIf AWi/AP=1 ± 0.1, then it is regarded as there are inhibiting effect, in acceptable toxicity；
If wastewater sample when detecting between not up to stationary phase within the scope of t2, or reach stationary phase, but AWi/AP＜ 0.9,
Then it is considered that there are lethal toxicities to microorganism, biological treatment system is had an impact.Test bacterium solution
The beneficial effects of the invention are as follows：
1, the method for the present invention can assess comprehensive toxicity of the waste water to water treatment micro-organism.
2, the method for the present invention comes from the activity of aerobic biological treatment process section using water treatment micro-organism as instruction biology
Sludge, the influence to evaluation waste water to Aerobic biological process system have more intuitive, practical application meaning bigger.
3, the method for the present invention has universality, evaluable to the bio-toxicity of different types of waste water, and by not
Same source, different extension rate waste water bio-toxicity testing result, waste water dual treatment and (reinforcings) can be instructed in advance
Processing.
4, the method for the present invention is simple and practicable, and operating condition is mild, and operating procedure is simple, and conventional detection apparatus is suitable for this
Invention.
Description of the drawings
Description of the drawings：
Fig. 1 is flow diagram of the waste water to the appraisal procedure of water treatment micro-organism toxicity of the present invention；
Fig. 2 is that the composite waste toxicity evaluation result of the present invention instructs the flow of strenuous primary treatment technique to lead figure；
Fig. 3 is that the sub-thread wastewater toxicity assessment result of the present invention instructs the flow of waste water dual treatment and strenuous primary treatment to lead
Figure.
Specific implementation mode
With reference to specific example, the present invention is further explained.It should be understood that these embodiments be merely to illustrate the present invention and
It is not used in and limits the scope of the invention.In addition, it should also be understood that, after having read the content that the present invention is lectured, people in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Culture medium, buffer solution, the composition of artificial wastewater and preparation in the present invention：
1, TSA culture mediums：Tryptone 15.0g/L, soy peptone 5.0g/L, sodium chloride 5.0g/L, agar 15.0g/
L, water；After weighing, heating stirring is dissolved in distilled water, and water is added to be settled to culture medium final volume, with pH meter test media
Acid-base value is adjusted pH value to 7.3 ± 0.2, in 121 DEG C of high pressure sterilization 20min with the HCl of the NaOH of 1mol/L or 1mol/L.
It by the TSA culture mediums after autoclave sterilization, is down flat plate while hot, is placed at room temperature for, cooled and solidified to obtain the final product.
2, TSB culture mediums：Tryptone 15.0g/L, soy peptone 5.0g/L, sodium chloride 5.0g/L, water；After weighing,
Heating stirring is dissolved in distilled water, and water is added to be settled to culture medium final volume, and pH value is adjusted to 7.3 ± 0.2, at 121 DEG C
High pressure sterilization 20min.
3, phosphate buffer (pH=7.8)：Dipotassium hydrogen phosphate 5.59g, potassium dihydrogen phosphate 0.41g are taken, water is added to be settled to
1000mL, filtering, sterilizes 30 minutes at 115 DEG C.
4, artificial wastewater：Glucose 150mg/L；Soluble starch 100mg/L；Sodium carboxymethylcellulose 50mg/L；Pancreas egg
White peptone 100mg/L；Ammonium sulfate 126mg/L；Potassium dihydrogen phosphate 31mg/L；Sodium bicarbonate 250mg/L, with constant volume after water dissolution.
The preparation of bacterium solution is tested in the present invention：
1) activated sludge that Aerobic biological process section is acquired from the sewage/wastewater treatment plant of normal operation, after sludge is fetched,
Fully break up mixing；
2) step dilution is carried out with sterile saline, fully shaking mixing is wanted in dilution, by different dilution ratios
Sludge, be inoculated on tablet, smoothen, 16-18h is cultivated in 20 DEG C of constant incubators,
3) start experiment when, by microorganism of the clump count on one group of tablet of 30-300 use under sterile washing afterwards mixing,
It is inoculated in TSB culture mediums, is configured to test bacterium solution.It tests absorbance of the bacterium solution at wavelength 450-650nm and reaches 0.3-
0.7。
Alternatively,
1) active sludge microorganism for taking out (- 80 DEG C) preservations of freezing, melts in 4 DEG C of refrigerators, and TSB culture mediums are added
In, it is put into shaken cultivation in 20 DEG C of shaken cultivation casees and is brought back to life；
2) bacterium solution after resurrection is inoculated on tablet, 16-18h is cultivated in 20 DEG C of constant incubators；
3) microorganism of the clump count on one group of tablet of 30-300 rear mixing under sterile washing is chosen, TSB is inoculated in
In culture medium, absorbance of the test bacterium solution at wavelength 450-650nm is obtained between 0.3-0.7.
The specific descriptions of turbidimetric assay in the present invention：
1) solution is prepared
Each 1mL of waste water sample of different extension rates is taken respectively, and the test bacterium solution that 9mL is prepared as stated above is then added,
Obtain mixed liquor to be measured, respectively set 5 it is parallel.In addition positive control and negative control is respectively set, positive control is that 1mL synthesis is dirty
Water is added 9mL and tests bacterium solution, and negative control is that 9mL is added without any test organisms in 1mL phosphate buffers (pH=7.8)
TSB culture mediums.
2) turbidimetric assay
Mixed liquor to be measured, negative control, positive control are added in cuvette, after cap, are put into together in advance
Heat simultaneously remains 35 DEG C of microorganism than in opacimetry instrument, being carried out at the same time turbidimetric assay, wherein setting location parameter：Culture temperature
Degree 35 DEG C, shake interval time 5min, single shake time 20s, shaking frequency be 0.2HZ, monitor time started 40min (from
Start the monitoring that 40min after testing carries out absorbance), monitor interval time 10min.After the completion of setting, 20s is shaken, is removed
Background values starts to test, total experimental period 4h (specifically being determined according to hydraulic detention time)；Monitoring wavelength is 530nm.
3) data processing
Data result is checked with the corresponding workstation software of instrument, and primary experiment provides 20 groups (when monitoring time is 4h)
The monitoring data of different time.Using the time as x-axis, using absorbance value as y-axis, growth curve is drawn.Compare positive control first
When into stationary phase t1, whether wastewater sample also enters stationary phase, if stationary phase, the then absorbance of wastewater sample when comparing t1
Value AWiWith the absorbance value A of positive controlPIf AWi/AP=1 ± 0.1, then it is regarded as no biotoxicity and (shows see assessment result
Meaning is one)；If positive control enters stationary phase t1, wastewater sample then compares detection time t2 (according to waterpower also not to stationary phase
Residence time determine) when wastewater sample absorbance value AWiWith the absorbance value A of positive controlPIf AWi/AP=1 ± 0.1, then
It is regarded as there are inhibiting effect, in acceptable toxicity (see assessment result signal two)；If wastewater sample when detecting between t2 models
It is not up to stationary phase in enclosing, or reaches stationary phase, but AWi/AP＜ 0.9, then it is assumed that be to microorganism there are lethal toxicity (see
Assessment result is illustrated three), to have an impact biological treatment system.
With reference to embodiment, the present invention is further illustrated：
Embodiment 1
Toxicity evaluation of certain pharmaceutical factory's composite waste to water treatment micro-organism
The composite waste W for choosing certain pharmaceutical factory, assesses its Ecotoxicology, and determination step is as follows：
(1) waste water W is filtered, in conjunction with sanitary sewage amount, dilutes three multiples with phosphate buffer solution (pH7.8)
(1,1.2,3)；
(2) the test bacterium solution that the absorbance at wavelength 530nm reaches 0.3-0.7 is prepared；
21) activated sludge of Aerobic biological process section is acquired from the sewage/wastewater treatment plant of normal operation, sludge is fetched
Afterwards, mixing is fully broken up；
22) step dilution is carried out with sterile saline, fully shaking mixing is wanted in dilution, by different dilution ratios
Sludge, be inoculated on tablet, smoothen, 16h is cultivated in 20 DEG C of constant incubators；
23) the rear mixing under sterile washing of the microorganism by clump count on one group of tablet of 30-300, is inoculated in TSB trainings
It supports in base, obtains test bacterium solution, the absorbance at wavelength 530nm is between 0.3-0.7；
It (3) will test bacterium solution and waste water water sample 9:1 mixing, is mixed with buffer solution using culture medium as negative control, with
Test bacterium solution is mixed with artificial wastewater is used as positive control.
(4) it is measured than opacimetry instrument by microorganism, records the absorbance value in 4h, draw the absorbance of each point
Value-time graph.
(5) Ecotoxicology of each stock waste water is evaluated according to data processing method, the results are shown in Table 1.It follows that system
Pharmaceutical factory waste water contains Biostatic substance and generates toxicity to water treatment micro-organism, influences microbiological treatment effect, it is therefore desirable to
Dual treatment or strenuous primary treatment are carried out to waste water.
The Ecotoxicology assessment result of each plant effluent of certain pharmaceutical factory of table 1.
Embodiment 2
Toxicity evaluation of each workshop waste water of certain pharmaceutical factory to water treatment micro-organism
The workshop of certain pharmaceutical factory totally 5 arterial drainages, each workshop discharge waste water water quantity and quality is different, and pharmaceutical factory need to be to it
Processing is carried out to reach discharge standard and drain into urban wastewater treatment firm.The Ecotoxicology of per share waste water is assessed, determination is
It is no that strenuous primary treatment is needed to carry out biochemical treatment again.Its step are as follows：
(1) several bursts of waste water (A1, A2, A3, A4, A5) are filtered, in conjunction with sanitary sewage amount, use phosphate-buffered respectively
Solution (pH7.8) dilutes three multiples (wherein, intermediate multiple is this strand of waste water ratio shared in total Water)；
(2) the test bacterium solution that the absorbance at wavelength 530nm reaches 0.3-0.7 is prepared；
21) activated sludge of Aerobic biological process section is acquired from the sewage/wastewater treatment plant of normal operation, sludge is fetched
Afterwards, mixing is fully broken up；
22) step dilution is carried out with sterile saline, fully shaking mixing is wanted in dilution, by different dilution ratios
Sludge, be inoculated on tablet, smoothen, 16h is cultivated in 20 DEG C of constant incubators；
23) the rear mixing under sterile washing of the microorganism by clump count on one group of tablet of 30-300, is inoculated in TSB trainings
It supports in base, obtains test bacterium solution, the absorbance at wavelength 530nm is between 0.3-0.7；
It (3) will test bacterium solution and waste water water sample 9:1 mixing, is mixed with buffer solution using culture medium as negative control, with
Test bacterium solution is mixed with artificial wastewater is used as positive control.
(4) it is measured than opacimetry instrument by microorganism, records the absorbance value in 4h, draw the absorbance of each point
Value-time graph.
(5) Ecotoxicology of each stock waste water is evaluated according to data processing method, the results are shown in Table 2.
The Ecotoxicology assessment result of each plant effluent of certain pharmaceutical factory of table 2.
Embodiment 3
Certain industrial park is machined factory's waste water, waste water from pesticide factory, the water outlet of waste water processing station of mineral wool factory to the micro- life of water process
The toxicity evaluation of object
Choose the water outlet of certain chemical industrial park waste water processing station of mechanical processing factory, waste water from pesticide factory treating stations are discharged, mineral wool factory is useless
Water treatment station is discharged, and is assessed its Ecotoxicology, determination step is as follows：
(1) by wastewater from mechanical industry (W1), agricultural chemicals waste water (W2), mineral wool waste water (W3) is filtered, in conjunction with sanitary sewage
Amount, using phosphate buffer solution (pH7.8) to dilute three multiples respectively, (wherein, intermediate multiple is this strand of waste water in total Water
Shared ratio)；
(2) the test bacterium solution that the absorbance at wavelength 530nm reaches 0.3-0.7 is prepared；
21) active sludge microorganism for taking out freezen protective, melts in 4 DEG C of refrigerators, is added in TSB culture mediums, is put into
Shaken cultivation is brought back to life in 20 DEG C of shaken cultivation casees；
22) bacterium solution after resurrection is inoculated on tablet, 16-18h is cultivated in 20 DEG C of constant incubators；
23) microorganism of the clump count on one group of tablet of 30-300 rear mixing under sterile washing is chosen, TSB is inoculated in
In culture medium, absorbance of the test bacterium solution at wavelength 530nm is obtained between 0.3-0.7.
It (3) will test bacterium solution and waste water water sample 9:1 mixing, is mixed with buffer solution using culture medium as negative control, with
Test bacterium solution is mixed with artificial wastewater is used as positive control.
(4) it is measured than opacimetry instrument by microorganism, records the absorbance value in 3h, draw the absorbance of each point
Value-time graph.
(5) Ecotoxicology of each stock waste water is evaluated according to data processing method, the results are shown in Table 3.
The Ecotoxicology assessment result of each enterprise wastewater in certain chemical industrial park of table 3.
In summary, the present invention is based on the basis that microbiological turbidity method measures, activated sludge (is come from environmental microorganism
In water treatment micro-organism) be instruction biology, by waste water to the inhibition level of microorganism, it is micro- to water process to establish assessment waste water
The method of bio-toxicity.Originally microbiological turbidity method is the important method for being used for detecting medicine preparation purity described in pharmacopeia, existing
Also it has been applied in waste water in the detection of certain substances (such as antibiotic) with bacteriostasis, these methods are all single with standard
Bacterial strain has no as biological subject using environment complex microorganism.The method of the present invention can accurate fast and convenient judgement it is useless
Influence of the water to biological treatment system, dual treatment and (reinforcing) pretreatment to waste water provide the guidance of directionality, to waste water
Be effectively treated, qualified discharge, poisonous substance emission reduction are of great significance.
Claims (8)
1. a kind of assessment waste water is to the method for water treatment micro-organism toxicity, which is characterized in that the method includes the following steps：
1) water treatment micro-organism test bacterium solution is prepared；
2) by more parts of wastewater dilution to be measured, every part of extension rate is different；
3) waste water after dilution is added to mixing in the water treatment micro-organism test bacterium solution prepared；
4) the microorganism increment in every part of mixed solution after mixing is timed in the given time or the real time measure with
Obtain every part of mixed solution microbial growth curve；
5) microbial growth curve will be measured to be compared with blank control.
2. method of the assessment waste water to water treatment micro-organism toxicity as described in claim 1, which is characterized in that the test
Absorbance of the bacterium solution at wavelength 450-650nm reaches 0.3-0.7.
3. method of the assessment waste water to water treatment micro-organism toxicity as described in claim 1, which is characterized in that at the water
Reason microorganism testing bacterium solution is prepared according to the following steps：
11) activated sludge for acquiring Aerobic biological process section, fully breaks up mixing；
12) activated sludge for breaing up mixing carries out step dilution；
13) sludge seeding after diluting cultivates 16-18h on tablet in 20 DEG C of constant incubators；
14) microorganism of the clump count on one group of tablet of 30-300 rear mixing under sterile washing is chosen, TSB cultures are inoculated in
In base.
4. method of the assessment waste water to water treatment micro-organism toxicity as claimed in claim 3, which is characterized in that the activity
If sludge cannot acquire acquisition in time, can water treatment micro-organism be subjected to freezen protective, before testing, test bacterium solution be by
Prepared by following step：
41) active sludge microorganism for taking out freezen protective, melts in 4 DEG C of refrigerators, is added in TSB culture mediums, is put into 20 DEG C
Shaken cultivation is brought back to life in shaken cultivation case；
42) bacterium solution after resurrection is inoculated on tablet, 16-18h is cultivated in 20 DEG C of constant incubators；
43) microorganism of the clump count on one group of tablet of 30-300 rear mixing under sterile washing is chosen, TSB cultures are inoculated in
In base, absorbance of the test bacterium solution at wavelength 450-650nm is obtained between 0.3-0.7.
5. method of the assessment waste water to water treatment micro-organism toxicity as described in claim 1, which is characterized in that the waste water water
Sample is to adopt that phosphate buffer solution is diluted, and the pH value of the phosphate buffer solution is 7-8.
6. method of the assessment waste water to water treatment micro-organism toxicity as described in claim 1, which is characterized in that the blank pair
According to using artificial wastewater, the artificial wastewater is made of following raw materials：Glucose 150mg/L；Soluble starch 100mg/L；
Sodium carboxymethylcellulose 50mg/L；Tryptone 100mg/L；Ammonium sulfate 126mg/L；Potassium dihydrogen phosphate 31mg/L；Sodium bicarbonate
250mg/L, with water dissolution constant volume.
7. method of the assessment waste water to water treatment micro-organism toxicity as described in claim 1, which is characterized in that micro- life
The measurement of object increment is using turbidimetric assay, step：
Every part of mixed solution, negative control, positive control after mixing is added in cuvette；
Each cuvette is put into together warmed-up and remains 35 DEG C of microorganism than in opacimetry instrument, being carried out at the same time than turbid survey
It is fixed；
Wherein, location parameter is set：35 DEG C of cultivation temperature shakes interval time 5min, and single shakes time 20s, shakes frequency
For 0.2HZ, monitoring time started 40min (monitoring of 40min progress absorbance after starting experiment), interval time is monitored
10min；Total experimental period 3-6h；Monitoring wavelength is 450-650nm.
8. method of the assessment waste water to water treatment micro-organism toxicity as claimed in claim 7, which is characterized in that described will survey
Determining the step of microbial growth curve is compared with blank control is：
When comparing positive control and entering stationary phase t1, whether wastewater sample also enters stationary phase：
If wastewater sample has been enter into stationary phase, the absorbance value A of wastewater sample when comparing t1WiWith the absorbance value of positive control
APIf AWi/AP=1 ± 0.1, then it is regarded as no biotoxicity；
If wastewater sample is not also to stationary phase, the absorbance value AWi and positive control of wastewater sample when comparing monitoring time t2
Absorbance value AP, if AWi/AP=1 ± 0.1, then it is regarded as there are inhibiting effect, in acceptable toxicity；
If wastewater sample when detecting between not up to stationary phase within the scope of t2, or reach stationary phase, but AWi/AP＜ 0.9, then recognize
To be that there are lethal toxicities to microorganism, biological treatment system is had an impact.
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| CN109975228A (en) * | 2019-05-10 | 2019-07-05 | 苏州华能检测技术有限公司 | A kind of wastewater toxicity detection method |
| CN110205356A (en) * | 2019-05-15 | 2019-09-06 | 杭州秀川科技有限公司 | A kind of differentiated management method of the pharmacy waste water based on MIC toxicity detection technology |
| CN110331183A (en) * | 2019-07-22 | 2019-10-15 | 天津大学 | The appraisal procedure of tetracycline and its catabolite to Escherichia coli eco-toxicity |
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| CN110343737A (en) * | 2019-07-22 | 2019-10-18 | 天津大学 | The appraisal procedure of sulfamethoxazole and its catabolite to Escherichia coli eco-toxicity |
| CN111855947A (en) * | 2020-08-10 | 2020-10-30 | 北京石油化工学院 | Toxicity detection method based on BOD difference value before and after degradation of activated sludge |
| CN113885767A (en) * | 2021-10-09 | 2022-01-04 | 中元汇吉生物技术股份有限公司 | Data display method of microorganism culture system, touch screen display and medium |
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