CN108375610A - A kind of bearing calibration for analyzing sludge enzymatic activity based on temperature inhibiting rate - Google Patents
A kind of bearing calibration for analyzing sludge enzymatic activity based on temperature inhibiting rate Download PDFInfo
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- CN108375610A CN108375610A CN201810047746.6A CN201810047746A CN108375610A CN 108375610 A CN108375610 A CN 108375610A CN 201810047746 A CN201810047746 A CN 201810047746A CN 108375610 A CN108375610 A CN 108375610A
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- 239000010802 sludge Substances 0.000 title claims abstract description 50
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 17
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 33
- 238000002474 experimental method Methods 0.000 claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 13
- 230000036284 oxygen consumption Effects 0.000 claims description 21
- 238000012937 correction Methods 0.000 claims description 14
- 231100000167 toxic agent Toxicity 0.000 claims description 14
- 239000003440 toxic substance Substances 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 6
- 238000004737 colorimetric analysis Methods 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 150000007513 acids Chemical class 0.000 claims description 4
- NESLWCLHZZISNB-UHFFFAOYSA-M sodium phenolate Chemical compound [Na+].[O-]C1=CC=CC=C1 NESLWCLHZZISNB-UHFFFAOYSA-M 0.000 claims description 4
- 239000006260 foam Substances 0.000 claims description 3
- 239000013558 reference substance Substances 0.000 claims description 3
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 10
- 238000012954 risk control Methods 0.000 abstract description 2
- 108010046334 Urease Proteins 0.000 description 12
- 239000010865 sewage Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- HEFNNWSXXWATRW-UHFFFAOYSA-N Ibuprofen Chemical compound CC(C)CC1=CC=C(C(C)C(O)=O)C=C1 HEFNNWSXXWATRW-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- VPOMSPZBQMDLTM-UHFFFAOYSA-N 3,5-dichlorophenol Chemical compound OC1=CC(Cl)=CC(Cl)=C1 VPOMSPZBQMDLTM-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000000177 Indigofera tinctoria Nutrition 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 231100000209 biodegradability test Toxicity 0.000 description 1
- 229940106691 bisphenol a Drugs 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229960001680 ibuprofen Drugs 0.000 description 1
- 229940097275 indigo Drugs 0.000 description 1
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002723 toxicity assay Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of bearing calibrations for analyzing sludge enzymatic activity based on temperature inhibiting rate.The present invention is corrected comparing to determine between being conducive to different tests by introducing standard substance as reference, saves experiment resource and effective time.Simultaneously, pass through the activity level of markization different batches sludge, be conducive to the comparativity between the stability and each result of test result, help to realize chemical producting safety assessment standardization, facilitate future to the management and application of chemicals, to biological treatment risk control and strick precaution.
Description
Technical field
The present invention relates to a kind of pollutants to contact sludge urease activity determination experiment bearing calibration.
Background technology
Activated sludge enzymatic activity and urban sewage treatment process operation conditions are closely related, and the activity of enzyme can be used as active dirt
The important indicator of mud performance and functional diversity, compared to biochemical indicators such as COD, BOD, TOC, enzyme index sensibility higher can
Important indicator as activated sludge performance and functional diversity.Wherein urase (URease) is a kind of to promote itrogenous organic substance
The hydrolase of hydrolysis, the single-minded hydrolysis urea of energy, is of great significance to the decomposition of itrogenous organic substance in sewage.
But the secondary settling tank sludge in sewage treatment plant often has anisotropism, due to different disposal factory sludge environment mechanism
Complexity, cause its composition also to have larger difference.Therefore different compounds needs to eliminate when observing sludge enzymatic activity
Difference between experiment.Therefore a kind of bearing calibration is needed, to realize the quality control between enzymatic activity experiment, to reach future studies
Increase the parallel comparativity of data in the process, realize activity data laboratory monitoring is comparable, different enzymatic activitys are comparable, different pollutants can
Than.
Science, the reliability of sludge biodegrading activity data are paid close attention to by various aspects.But for various reasons, mesh
The rapid biodegradability test data that some preceding mechanism for testing are provided is of low quality, affects the science of identification and assessment result
Property.Such as the municipal sewage plant of different regions, microbial can have differences；Same city is in the dirt of different operating statuses
The difference of water treatment plant, microbial is also very big.Therefore trans-regional standard inoculation object is researched and developed as early as possible, it is real to gained sludge activity
It tests data and carries out parameter correction, to realize the standardization of the comparativity and chemical producting safety evaluation studies of test result.
Invention content
It is of low quality to make up existing biodegradable enzymatic activity test data, such as the municipal sewage treatment of different regions
Factory, enzymatic activity data difference is big, affects the scientific deficiency of identification and assessment result, and the present invention provides a kind of sludge enzyme activity
Property bearing calibration.
This approach includes the following steps：
1) reference substance is prepared
Standard toxicant, configuration 3,5- are used as using 3,5- chlorophenesic acids (3,5-dichlorophenol, 3,5-DCP)
Chlorophenesic acid solution, makes it dissolve, at room temperature constant volume, and concentration is in 0~50mgL- 1Set in range at 1 points；
2) measurement of oxygen consumption rate
At room temperature, by activated sludge and 3,5-DCP is mixed in equal volume, is placed in conical flask and is fully aerated, when ρ (DO) reaches
It is inserted into DO electrodes when saturation state, on-line checking mixed liquor ρ (DO) and is recorded under conditions of ensureing good air-tightness；
According to DO versus time curves, the slope in linearly interval is calculated, which is oxygen consumption rate R [mg
(L·h)- 1]；
Activated sludge is not added using under matched samples dosage as abiotic control, on-line checking ρ (DO), according to DO with
The change curve of time obtains abiotic control oxygen consumption rate R1[mg·(L·h)- 1]；
The activity that activated sludge is investigated not add 3,5-DCP as blank control, obtains blank control oxygen consumption rate R0
[mg·(L·h)- 1]；
It is to active sludge oxygen-consuming rate inhibiting rate I (%) under a certain concentration of standard toxicant：
I=[1- (R-R1)/R0]×100；
Using under a certain concentration of standard toxicant to the oxygen consumption rate inhibiting rate I of activated sludge as ordinate, with standard poison
Property material concentration (mgL- 1) logarithm be abscissa, draw concentration effect curve, then to data carry out fitting a straight line,
And obtain slope of curve k；
3) it corrects
Enzyme classes activity experiment is carried out with corresponding manner, as sodium phenate colorimetric method surveys urease activity, 3,5- dinitrosalicylics
Acid system surveys invertase activity and surveys dehydrogenase activity with triphenyltetrazolium chloride colorimetric method.It is the reaction generated that experiment, which measures result,
The reaction product quality of product quality, the generation measured is wanted divided by mud dry weight, is produced with the reaction that 1g dry weight activated sludge generates
Amount of substance indicates enzymatic activity.With the k ' reciprocal of step 2) concentration effect curve slope for correction parameter, sludge enzymatic activity after correction
=actual measurement sludge enzymatic activity k '.
More specifically, activated sludge mixes 3 hours in equal volume with 3,5-DCP of various concentration respectively in the step 2),
It is aerated 30min when mixed liquor is thoroughly mixed and non-foam is overflowed, mixed liquor is transferred in 250ml conical flasks immediately after,
It is inserted into DO electrodes.
Advantageous effect
The present invention is corrected comparing to determine between being conducive to different tests by introducing standard substance as reference, saves examination
Certification of registered capital source and effective time.Meanwhile by the activity level of markization different batches sludge, being conducive to the stabilization of test result
Property and each result between comparativity, help to realize the standardization of chemical producting safety assessment, facilitate the following pipe to chemicals
It manages and applies, to biological treatment risk control and strick precaution.
Description of the drawings
Fig. 1 is the dense of 3,5-DCP concentration and the oxygen consumption rate inhibiting rate of two kinds of different batches sludge in the embodiment of the present invention
Degree-effect curve.X is logarithm, that is, lg (C-DCP) of 3,5-DCP concentration in figure, and y is oxygen consumption rate inhibiting rate I.By to data
It carries out linear fit and obtains fit equation y=kx+b and corresponding coefficient k.
Fig. 2 is not use the urease activity measured by sodium phenate colorimetric method when parameter correction real in the embodiment of the present invention
Test data.Horizontal axis is ibuprofen concentration in figure, and the longitudinal axis is the urease activity measured.
Fig. 3 is in the embodiment of the present invention using the urease activity experimental data after parameter correction.Horizontal axis is brufen in figure
Concentration, the longitudinal axis are urease activity after correction.
Specific implementation mode
The present invention is described in detail below by specific embodiment, but is not limited the scope of the invention.Unless otherwise specified, originally
Experimental method is conventional method used by invention, and experiment equipment used, material, reagent etc. can chemically company be bought.
Embodiment 1
1) reference substance is prepared
Using 3,5-DCP as standard toxicant.Configure 1000mgL- 13,5-DCP solution 500mL, by adding
Hot method promotes its dissolving, and constant volume at room temperature.It is diluted to a concentration of 0.1,0.4,1.6,6.3,25mg/L.
2) measurement of oxygen consumption rate
By activated sludge and 3,5-DCP is mixed in equal volume, is placed in the conical flask that several dischargeable capacitys are 250mL and is fully exposed
Gas.Experiment mixed liquor total volume is 50mL, and wherein activated sludge inoculation liquid 25mL, remaining 25mL can add the 3,5- of various concentration
DCP, mixed liquor contact 3 hours.It is aerated 30min under conditions of keeping mixed liquor to be thoroughly mixed and not spilling over foam.Then
Mixed liquor is transferred in conical flask immediately, oxygen electrode is inserted into, continues to monitor 10min.
Before each mixed liquor measures, by not adding activated sludge (replacing activated sludge using deionized water) as non-life
Object compares, and determines that 3,5-DCP solution itself does not have the characteristic for putting oxygen or oxygen consumption；The toxicity assay each time of mixed liquor is all
With corresponding abiotic check experiment under equal 3, a 5-DCP dosage, pass through abiotic check experiment result deduction pair
Toxicity test result is corrected.
DO versus time curves are measured according to experiment, calculate the slope in its linearly interval, i.e. oxygen consumption rate R
[mg·(L·h)- 1]。
It is to active sludge oxygen-consuming rate inhibiting rate I (%) under a certain concentration of standard toxicant：
I=[1- (R-R1)/R0]×100
In formula, R, R0And R1Standard toxicant contact test, blank control test and abiotic control examination are indicated respectively
Oxygen consumption rate [the mg (Lh) measured in testing- 1]。
Using standard toxicant to the oxygen consumption rate inhibiting rate I of activated sludge as ordinate, with standard toxicant concentration
(mg·L- 1) logarithm be abscissa, draw concentration effect curve, fitting a straight line then carried out to data, and obtains curve
Slope.
3) sodium phenate colorimetric method for determining urease activity is applied
By activated sludge and 0~50 μ gL-1Concentration gradient bisphenol-A is mixed into the mixed liquor of 100mL in equal volume.Sludge takes
From Dalian sewage treatment plant, first group of sludge was fetched on November 15th, 2018, and second group of sludge was on December 28th, 2018
It fetches.Mixed sludge exposes 15h respectively, and 5mL sludge suspension and 1mL toluene are sufficiently mixed, and it is molten that 10% urea is added
Liquid and citric acid solution are placed in 37 DEG C of constant incubators and cultivate for 24 hours.Mixed liquor is filtered after taking-up.Take 3mL filtrates according to
Secondary addition 4mL 0.28mol/L sodium phenates and 1% sodium hypochlorite of 3mL are stood after mixing, and indigo is presented in solution, after dilution in
578nm surveys absorbance.With NH in rear 1g activated sludge for 24 hours3The milligram number of-N indicates urease activity.It deducts because of each water content
It is not fixed the influence of i.e. sludge concentration sludge, dry weight.To be deducted with no Matrix controls data result without soil control under the same terms
It is influenced to eliminate non-biological moieties.
4) it corrects
Oxygen consumption rate and sludge concentration, the negatively correlated relationship of activity, similarly oxygen consumption rate inhibiting rate is at sludge concentration, activity
Negatively correlated relationship, with the k ' reciprocal of standard toxicant respiration inhibition experimental concentration-effect curve slope for correction parameter.
The urease activity data measured are multiplied by correction parameter and carry out enzymatic activity correction.
Urease activity=actual measurement urease activity k ' after correction
Relative standard deviation (RSD, relative standard deviation), i.e.,：Relative standard deviation (RSD)=
The arithmetic mean of instantaneous value (X) * 100% of standard deviation (SD)/result of calculation
According to fig. 2 with Fig. 3 it is found that data RSD is 14.2% before correction, RSD is 7% after correction, and data precision improves,
So experiment reproducibility improves.
The preferable specific implementation mode of the above, only the invention, but the protection domain of the invention is not
It is confined to this, any one skilled in the art is in the technical scope that the invention discloses, according to the present invention
The technical solution of creation and its inventive concept are subject to equivalent substitution or change, should all cover the invention protection domain it
It is interior.
Claims (5)
1. a kind of bearing calibration for analyzing sludge enzymatic activity based on temperature inhibiting rate, which is characterized in that include the following steps：
1) reference substance is prepared
Using 3,5- chlorophenesic acids as standard toxicant, configuration 3,5- chlorophenesic acids solution, constant volume, concentration 0~
50mg·L- 1Set in range at 1 points；
2) measurement of oxygen consumption rate
At room temperature, by activated sludge and 3,5- chlorophenesic acids mix, are placed in conical flask and are fully aerated in equal volume, when ρ (DO) reaches
To DO electrodes are inserted into when saturation state, on-line checking mixed liquor ρ (DO) and recorded under conditions of ensureing good air-tightness；
It is to active sludge oxygen-consuming rate inhibiting rate I (%) under a certain concentration of standard toxicant：
I=[1- (R-R1)/R0]×100；
Wherein, R is standard toxicant contact test oxygen consumption rate [mg (Lh)- 1]；
R0For blank control test oxygen consumption rate [mg (Lh)- 1]；
R1For abiotic check experiment oxygen consumption rate [mg (Lh)- 1]；
Using under a certain concentration of standard toxicant to the oxygen consumption rate inhibiting rate I of activated sludge as ordinate, with standard toxic substance
Matter concentration (mgL- 1) logarithm be abscissa, draw concentration effect curve, then to data carry out fitting a straight line, and
Go out slope of curve k；
3) it corrects
Enzymatic activity experiment is carried out, with the k ' reciprocal of step 2) concentration effect curve slope for correction parameter, sludge enzyme activity after correction
Property=actual measurement sludge enzymatic activity k '.
2. according to the method described in claim 1, it is characterized in that, the R1It is not add activity under matched samples dosage
Sludge obtains abiotic control oxygen consumption speed as abiotic control, on-line checking ρ (DO) according to DO versus time curves
Rate.
3. according to the method described in claim 1, it is characterized in that, the R0It is to be examined using not adding 3,5-DCP as blank control
The activity for examining activated sludge obtains blank control oxygen consumption rate.
4. according to the method described in claim 1, it is characterized in that, in step 2) activated sludge respectively with various concentration 3,5-
DCP is mixed 3 hours in equal volume, and 30min is aerated when mixed liquor is thoroughly mixed and non-foam is overflowed, and immediately after turns mixed liquor
It moves in 250ml conical flasks, is inserted into DO electrodes.
5. according to the method described in claim 1, it is characterized in that, enzymatic activity experiment has sodium phenate colorimetric method, 3,5- bis-
Nitrosalicylic acid system and triphenyltetrazolium chloride colorimetric method.
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| CN201810047746.6A CN108375610B (en) | 2018-01-18 | 2018-01-18 | Correction method for analyzing sludge enzyme activity based on oxygen consumption rate inhibition rate |
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| CN201810047746.6A CN108375610B (en) | 2018-01-18 | 2018-01-18 | Correction method for analyzing sludge enzyme activity based on oxygen consumption rate inhibition rate |
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| Publication Number | Publication Date |
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| CN108375610A true CN108375610A (en) | 2018-08-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113686839A (en) * | 2021-08-23 | 2021-11-23 | 大连民族大学 | Method for synchronously evaluating removal and toxicity reduction of organic phosphate in sewage aerobic process |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09184845A (en) * | 1995-12-30 | 1997-07-15 | Shimadzu Corp | Automatic analyzing apparatus for measurement of enzyme activity |
| CN1234448A (en) * | 1998-12-30 | 1999-11-10 | 王太重 | Correction method for enzyme activity determination system and correction agent thereof |
| CN101358230A (en) * | 2008-09-05 | 2009-02-04 | 东莞宝丽美化工有限公司 | Method for measuring carboxymethylcellulose enzyme activity |
| JP2013129617A (en) * | 2011-12-21 | 2013-07-04 | Ichimaru Pharcos Co Ltd | Tryptase activity inhibitor |
| CN106348427A (en) * | 2016-09-20 | 2017-01-25 | 西安建筑科技大学 | Method for assessing whether activated sludge adapts to new environment or not based on breath graph |
| CN206399878U (en) * | 2016-11-08 | 2017-08-11 | 南京沙夫特环境科技有限公司 | A kind of device for detecting chemicals to activated sludge respiration inhibition toxicity |
-
2018
- 2018-01-18 CN CN201810047746.6A patent/CN108375610B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09184845A (en) * | 1995-12-30 | 1997-07-15 | Shimadzu Corp | Automatic analyzing apparatus for measurement of enzyme activity |
| CN1234448A (en) * | 1998-12-30 | 1999-11-10 | 王太重 | Correction method for enzyme activity determination system and correction agent thereof |
| CN101358230A (en) * | 2008-09-05 | 2009-02-04 | 东莞宝丽美化工有限公司 | Method for measuring carboxymethylcellulose enzyme activity |
| JP2013129617A (en) * | 2011-12-21 | 2013-07-04 | Ichimaru Pharcos Co Ltd | Tryptase activity inhibitor |
| CN106348427A (en) * | 2016-09-20 | 2017-01-25 | 西安建筑科技大学 | Method for assessing whether activated sludge adapts to new environment or not based on breath graph |
| CN206399878U (en) * | 2016-11-08 | 2017-08-11 | 南京沙夫特环境科技有限公司 | A kind of device for detecting chemicals to activated sludge respiration inhibition toxicity |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113686839A (en) * | 2021-08-23 | 2021-11-23 | 大连民族大学 | Method for synchronously evaluating removal and toxicity reduction of organic phosphate in sewage aerobic process |
| CN113686839B (en) * | 2021-08-23 | 2024-05-28 | 大连民族大学 | Method for synchronously evaluating removal of organic phosphate and toxicity reduction of organic phosphate in sewage aerobic process |
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| CN108375610B (en) | 2020-02-14 |
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