CN106348427A - Method for assessing whether activated sludge adapts to new environment or not based on breath graph - Google Patents
Method for assessing whether activated sludge adapts to new environment or not based on breath graph Download PDFInfo
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- 239000010802 sludge Substances 0.000 title claims abstract description 183
- 238000000034 method Methods 0.000 title claims abstract description 50
- 230000029058 respiratory gaseous exchange Effects 0.000 claims abstract description 78
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000010865 sewage Substances 0.000 claims abstract description 22
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 18
- 230000000630 rising effect Effects 0.000 claims abstract description 13
- 230000007613 environmental effect Effects 0.000 claims abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 24
- 239000001301 oxygen Substances 0.000 claims description 24
- 229910052760 oxygen Inorganic materials 0.000 claims description 24
- 230000000694 effects Effects 0.000 claims description 20
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 17
- 239000000460 chlorine Substances 0.000 claims description 17
- 229910052801 chlorine Inorganic materials 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 14
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- 238000003756 stirring Methods 0.000 claims description 8
- 239000008399 tap water Substances 0.000 claims description 8
- 235000020679 tap water Nutrition 0.000 claims description 8
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical group NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
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- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
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- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000001727 glucose Nutrition 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
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- 238000004140 cleaning Methods 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- -1 nitrogen-containing compound Chemical class 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims 4
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- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 125000001297 nitrogen containing inorganic group Chemical group 0.000 description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
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- 239000002028 Biomass Substances 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
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- 238000001514 detection method Methods 0.000 description 2
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- 230000000241 respiratory effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical group O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 108010020056 Hydrogenase Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 238000005660 chlorination reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
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- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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Abstract
本发明公开了一种基于呼吸图谱评估活性污泥是否适应新环境的方法,包括:1)对污水处理厂活性污泥进行呼吸图谱分析,得到两段呼吸图谱DO‑t曲线;2)对每段呼吸图谱DO‑t曲线分别拟合得到其斜率,得到内源呼吸速率OURe、及加氮源、碳源后的总呼吸速率OURt;3)得到OURe/OURt‑t曲线;4)根据OURe/OURt‑t曲线的变化趋势,评估活性污泥是否适应新环境,曲线呈先上升后平稳或先上升后下降趋势,预示活性污泥可以适应新环境;若曲线在改变环境条件一周后仍上升,即呈持续上升趋势,预示活性污泥无法适应新环境。本发明根据对活性污泥内源呼吸比(OURe/OURt)趋势的判断,简单有效地对活性污泥是否适应新环境进行了评估。The invention discloses a method for assessing whether activated sludge adapts to a new environment based on a respiration pattern, comprising: 1) analyzing the respiration pattern of the activated sludge in a sewage treatment plant to obtain two sections of the respiration pattern DO-t curve; 2) analyzing each The DO-t curve of the segmental respiration spectrum is fitted to obtain its slope, and the endogenous respiration rate OURe, and the total respiration rate OURt after adding nitrogen source and carbon source are obtained; 3) the OURe/OURt-t curve is obtained; 4) according to the OURe/OURt-t curve, The change trend of the OURt-t curve is used to evaluate whether the activated sludge adapts to the new environment. The curve shows a trend of rising first and then stabilizing or rising and then falling, which indicates that the activated sludge can adapt to the new environment; if the curve still rises after changing the environmental conditions for a week, That is, it showed a continuous upward trend, indicating that activated sludge could not adapt to the new environment. According to the judgment of the trend of the endogenous respiration ratio (OURe/OURt) of the activated sludge, the invention simply and effectively evaluates whether the activated sludge adapts to the new environment.
Description
技术领域technical field
本发明属于污水处理领域,涉及一种通过对活性污泥呼吸图谱的分析,评估活性污泥是否适应新环境的方法。The invention belongs to the field of sewage treatment and relates to a method for evaluating whether activated sludge adapts to a new environment by analyzing activated sludge respiration patterns.
背景技术Background technique
活性污泥工艺是目前应用最为广泛的污水处理技术,占城市污水处理工艺的90%以上和工业废水处理工艺的50%左右。而活性污泥工艺的核心就是活性污泥,污废水处理效果的优劣取决于活性污泥中微生物的特性,然而环境条件的变化往往会影响活性污泥结构和活性的变化,从而影响污废水的处理效果。由此,了解活性污泥对环境变化的耐受程度,或者说评估活性污泥是否适应新环境就显得尤为重要。Activated sludge process is currently the most widely used sewage treatment technology, accounting for more than 90% of urban sewage treatment processes and about 50% of industrial wastewater treatment processes. The core of the activated sludge process is activated sludge. The effect of sewage and wastewater treatment depends on the characteristics of the microorganisms in the activated sludge. However, changes in environmental conditions often affect the changes in the structure and activity of activated sludge, thereby affecting sewage and wastewater. processing effect. Therefore, it is particularly important to understand the tolerance of activated sludge to environmental changes, or to evaluate whether activated sludge can adapt to the new environment.
目前,大量研究主要集中在活性污泥的活性测定上,而鲜有对活性污泥是否适应新环境进行评估的方法。测定活性污泥活性的方法有很多,包括活性污泥ATP含量测定法、活性污泥耗氧速率测定法、以及脱氢酶活性法等。活性污泥中ATP含量比较稳定,生物的生长速度不会影响ATP/活生物量比例,生物死亡后ATP马上消失。ATP的浓度与活性污泥生物量直接相关,也与活性污泥微生物的新陈代谢速度直接相关。高活性的活性污泥,微生物具有高新陈代谢速度,细胞内有高浓度的ATP;活性污泥耗氧速率(OUR)可以提供活性污泥活性的基本信息,当OUR值为20~40g O2/(kgVSS·h)时,表明活性污泥具有活性,若OUR值为5~10g O2/(kgVSS·h)时,其值较低,表明活性污泥活性较差;脱氢酶活性法是利用脱氢酶催化有机物脱氢,然后氢被传递给特定的受氢体(在好氧处理时,最后的受氢体是分子氧),而废水的生物处理就是利用微生物的作用而使废水中的有机物分解为较为简单的化合物,因此,脱氢酶的活性也代表了活性污泥的活性。但是以上这些都是对活性污泥活性的测定,目前并没有直接的方法评估活性污泥是否适应新环境。At present, a large number of studies mainly focus on the determination of the activity of activated sludge, but there are few methods to evaluate whether activated sludge can adapt to the new environment. There are many methods for measuring activated sludge activity, including activated sludge ATP content determination method, activated sludge oxygen consumption rate determination method, and dehydrogenase activity method. The ATP content in activated sludge is relatively stable, and the growth rate of organisms will not affect the ratio of ATP/living biomass, and ATP will disappear immediately after the organisms die. The concentration of ATP is directly related to the biomass of activated sludge, and also directly related to the metabolic rate of activated sludge microorganisms. Highly active activated sludge, microorganisms have a high metabolic rate, and there is a high concentration of ATP in the cells; the activated sludge oxygen consumption rate (OUR) can provide basic information on the activity of activated sludge, when the OUR value is 20 ~ 40g O2/( kgVSS·h), it indicates that the activated sludge is active, and if the OUR value is 5-10g O2/(kgVSS·h), its value is low, indicating that the activity of the activated sludge is poor; the dehydrogenase activity method uses dehydrogenase Hydrogenase catalyzes the dehydrogenation of organic matter, and then the hydrogen is transferred to a specific hydrogen acceptor (in aerobic treatment, the last hydrogen acceptor is molecular oxygen), and the biological treatment of wastewater is to use the action of microorganisms to make the organic matter in wastewater Decomposed into simpler compounds, therefore, the activity of dehydrogenase also represents the activity of activated sludge. However, all of the above are the determination of the activity of activated sludge, and there is no direct method to evaluate whether activated sludge can adapt to the new environment.
再加上在现有的中小型污水处理厂中,由于人才、设备的缺乏,无法简单、快捷的对环境(如:DO、温度、pH、化学药剂添加量等)条件变化下活性污泥是否能适应环境的改变,进行准确的评估和判断。基于以上现状,提出一种方法,即通过呼吸图谱分析建立了一种简单易行的评估活性污泥是否适应新环境的方法成为目前本领域亟待解决的技术问题。In addition, in the existing small and medium-sized sewage treatment plants, due to the lack of talents and equipment, it is impossible to simply and quickly check whether the activated sludge is activated under the changing conditions of the environment (such as: DO, temperature, pH, chemical agent addition, etc.) Ability to adapt to changes in the environment and make accurate assessments and judgments. Based on the above situation, it is an urgent technical problem to be solved in this field to propose a method, that is, to establish a simple and feasible method for evaluating whether the activated sludge can adapt to the new environment through the analysis of the breath pattern.
发明内容Contents of the invention
本发明的目的在于提供一种基于呼吸图谱评估活性污泥是否适应新环境的方法,该方法利用好氧条件下获得活性污泥呼吸图谱DO-t曲线,通过拟合得到其斜率,继而绘制出OURe/OURt-t的一条曲线,由OURe/OURt-t曲线的变化趋势,准确评估活性污泥是否适应环境。The purpose of the present invention is to provide a method for assessing whether activated sludge adapts to the new environment based on the respiration spectrum. The method utilizes the DO-t curve of activated sludge respiration spectrum obtained under aerobic conditions, obtains its slope by fitting, and then draws out A curve of OURe/OURt-t, from the changing trend of the OURe/OURt-t curve, accurately evaluates whether the activated sludge is suitable for the environment.
作为一种评估活性污泥是否适应环境的方法,污水厂活性污泥测试结果表明:我们发明的基于呼吸图谱评估活性污泥是否适应新环境的方法能够准确、高效的对活性污泥是否适应新环境进行评估。As a method to evaluate whether activated sludge can adapt to the environment, the test results of activated sludge in sewage plants show that: the method we invented to evaluate whether activated sludge can adapt to the new environment based on the respiration map can accurately and efficiently assess whether the activated sludge can adapt to the new environment. The environment is assessed.
本发明的目的是通过下述技术方案来实现的。The purpose of the present invention is achieved through the following technical solutions.
1.根据本发明实施例提供的一种基于呼吸图谱评估活性污泥是否适应新环境的方法,该方法包括以下步骤:1. a kind of method that assesses whether activated sludge adapts to new environment based on respiration pattern that the embodiment of the present invention provides, the method may further comprise the steps:
1)取污水处理厂活性污泥,用缓冲溶液对活性污泥进行多次清洗,并且充分曝气,使活性污泥进入内源呼吸状态;1) Take the activated sludge from the sewage treatment plant, wash the activated sludge multiple times with a buffer solution, and fully aerate the activated sludge to enter the endogenous breathing state;
2)在活性污泥进入内源呼吸状态后,充分搅拌,得到第一段呼吸图谱DO-t曲线,拟合得到其斜率,即为活性污泥内源呼吸速率OURe;2) After the activated sludge enters the state of endogenous respiration, it is fully stirred to obtain the DO-t curve of the first section of the respiration map, and the slope is obtained by fitting, which is the endogenous respiration rate OURe of the activated sludge;
3)在步骤2)活性污泥中分别加入氮源和碳源,然后充分曝气至溶氧DO达7mg/L以上,充分搅拌,得到另一段呼吸图谱DO-t曲线,拟合得到其斜率,即为总呼吸速率OURt;3) Add a nitrogen source and a carbon source to the activated sludge in step 2), then fully aerate until the dissolved oxygen DO reaches more than 7mg/L, and fully stir to obtain another section of the breath spectrum DO-t curve, and obtain its slope by fitting , which is the total respiration rate OURt;
4)重复步骤1)-3),分别得到随时间变化的内源呼吸速率OURe值与污泥总呼吸速率OURt值;4) Repeat steps 1)-3) to obtain the time-varying endogenous respiration rate OURe value and the total sludge respiration rate OURt value respectively;
5)用OURe/OURt比值绘制关于时间t的曲线,根据OURe/OURt-t曲线的变化趋势,评估活性污泥是否适应新环境,若曲线呈先上升后平稳或先上升后下降趋势,预示活性污泥可以适应新环境;若曲线呈持续上升趋势,预示活性污泥不适应新环境;5) Use the OURe/OURt ratio to draw a curve about time t, and evaluate whether the activated sludge adapts to the new environment according to the change trend of the OURe/OURt-t curve. If the curve rises first and then stabilizes or first rises and then declines, it indicates the activity The sludge can adapt to the new environment; if the curve shows a continuous upward trend, it indicates that the activated sludge cannot adapt to the new environment;
所述可适应的新环境包括:活性污泥从正常溶氧转为低溶氧的环境;活性污泥中加氯量为低氯量的环境;The adaptable new environment includes: the environment in which the activated sludge changes from normal dissolved oxygen to low dissolved oxygen; the environment in which the amount of chlorine added in the activated sludge is a low chlorine amount;
所述活性污泥不适应的新环境包括:活性污泥中加氯量为高氯量的环境。The new environment to which the activated sludge is not suitable includes: an environment in which the amount of chlorine added to the activated sludge is high.
进一步,所述步骤1)中,活性污泥进入内源呼吸状态的具体过程为:Further, in the step 1), the specific process for the activated sludge to enter the endogenous respiration state is:
取污水处理厂的活性污泥300ml,并用体积比1:3的比例添加自来水对泥进行稀释,将活性污泥样品通过搅拌、沉淀、去上清液、定容至活性污泥稀释液的1/2体积,然后用PBS缓冲溶液以0.2ml/s,进3s洗涤活性污泥一次,如此重复洗泥3次,之后添加自来水将活性污泥混合液定容至活性污泥稀释液的原体积,即1.2L,曝气2h,活性污泥进入内源呼吸状态。Take 300ml of activated sludge from the sewage treatment plant, and add tap water at a volume ratio of 1:3 to dilute the sludge. The activated sludge sample is stirred, precipitated, supernatant removed, and the volume is adjusted to 1% of the activated sludge dilution. /2 volume, then wash the activated sludge once with PBS buffer solution at 0.2ml/s for 3s, repeat the mud washing for 3 times, then add tap water to adjust the volume of the activated sludge mixture to the original volume of the activated sludge dilution , that is, 1.2L, aeration for 2h, the activated sludge enters the endogenous respiration state.
进一步,所述步骤2)、3)中,呼吸图谱DO-t曲线通过下述条件控制获得:Further, in the steps 2), 3), the breath spectrum DO-t curve is obtained through the following conditional control:
控制DO下降阶段持续时间t:5≤t≤25min和DO下降阶段变化范围:6.0≤DO≤4.0mg/L。Control the duration of the DO decline phase t: 5≤t≤25min and the variation range of the DO decline phase: 6.0≤DO≤4.0mg/L.
进一步,步骤2)、3)中获得呼吸图谱DO-t曲线过程的温度控制为8~45℃。Further, in steps 2) and 3), the temperature control in the process of obtaining the DO-t curve of the respiration map is 8-45°C.
进一步,步骤1)中,所述清洗污泥的缓冲溶液为PBS缓冲溶液,该缓冲溶液为以下组分混合液:Further, in step 1), the buffer solution for cleaning the sludge is a PBS buffer solution, which is a mixed solution of the following components:
A:KH2PO4浓度为1.5~2.5mmol·L-1;A: The concentration of KH 2 PO 4 is 1.5-2.5 mmol·L -1 ;
B:Na2HPO4浓度为8~12mmol·L-1;B: The concentration of Na 2 HPO 4 is 8-12 mmol·L -1 ;
C:NaCl浓度为135~140mmol·L-1;C: NaCl concentration is 135-140 mmol·L -1 ;
D:KCl浓度为2.5~3.0mmol·L-1。D: KCl concentration is 2.5-3.0 mmol·L -1 .
进一步,步骤3)中所述对污泥加入氮源按质量比每1g活性污泥中加入3~14mg氮元素质量含氮化合物。Further, add the nitrogen source to the sludge in step 3) and add 3-14 mg of nitrogen element and nitrogen-containing compound per 1 g of activated sludge in a mass ratio.
进一步,步骤3)中所述对污泥加入氮源为含氮元素无机物或含氮元素有机物,所述含氮元素无机物为氯化铵或硫酸铵;所述含氮元素有机物为尿素。Further, the nitrogen source added to the sludge in step 3) is nitrogen-containing inorganic matter or nitrogen-containing organic matter, and the nitrogen-containing inorganic matter is ammonium chloride or ammonium sulfate; the nitrogen-containing organic matter is urea.
进一步,步骤3)中所述对污泥加入碳源按质量比每1g活性污泥中加入200~700mg含碳元素有机物;所述碳源为单一碳源:乙酸钠、葡萄糖,或淀粉。Further, in step 3), add a carbon source to the sludge according to the mass ratio of 200-700 mg of carbon-containing organic matter per 1 g of activated sludge; the carbon source is a single carbon source: sodium acetate, glucose, or starch.
进一步,所述投加氮源与碳源的质量比按照干活性污泥比例投加。Further, the mass ratio of the nitrogen source to the carbon source is added according to the ratio of dry activated sludge.
进一步,所述正常溶氧DO为2-4mg/L,所述低溶氧为DO<0.5mg/L;Further, the normal dissolved oxygen DO is 2-4mg/L, and the low dissolved oxygen is DO<0.5mg/L;
所述低氯量为活性污泥中NaClO≤8‰,高氯量为活性污泥中NaClO≥15‰。The low chlorine content is NaClO≤8‰ in the activated sludge, and the high chlorine content is NaClO≥15‰ in the activated sludge.
进一步,所述活性污泥是否适应新环境的判定方法为:Further, whether described activated sludge adapts to the judging method of new environment is:
根据OURe/OURt-t曲线的变化趋势判断活性污泥是否适应新环境:①曲线呈先上升后平稳或先上升后下降趋势,预示活性污泥可以适应新环境;②曲线在环境条件改变一周后仍在上升,即呈持续上升趋势,说明内源呼吸速率OURe在增加,相应的功能性菌群的呼吸速率在减弱,预示活性污泥无法适应新环境,功能性菌群的活性将持续下降直至消失,最终解体;③曲线呈下降趋势,预示新环境有利于功能性菌群的生存,有利于活性污泥的生长。Judging whether the activated sludge adapts to the new environment according to the change trend of the OURe/OURt-t curve: ①The curve shows a trend of rising first and then stabilizing or rising first and then falling, indicating that the activated sludge can adapt to the new environment; ②The curve is changed after one week of environmental conditions It is still rising, that is, showing a continuous upward trend, indicating that the endogenous respiration rate OURe is increasing, and the respiration rate of the corresponding functional flora is weakening, indicating that the activated sludge cannot adapt to the new environment, and the activity of the functional flora will continue to decline until Disappear and eventually disintegrate; ③The curve shows a downward trend, indicating that the new environment is conducive to the survival of functional flora and the growth of activated sludge.
本发明具有以下优点:The present invention has the following advantages:
1)本发明找到呼吸图谱中内源呼吸速率OURe与总呼吸速率OURt比值(OURe/OURt)随时间t的变化趋势与活性污泥适应新环境与否之间的关系,可以准确评估活性污泥是否适应新环境,进而为解决指示活性污泥适应新环境的指标提供了有效的判定方法,具有一定的指导意义。1) The present invention finds the relationship between the change trend of the endogenous respiration rate OURe and the total respiration rate OURt ratio (OURe/OURt) over time t in the respiration map and whether the activated sludge adapts to the new environment, and the activated sludge can be accurately evaluated Whether it adapts to the new environment provides an effective judgment method for solving the indicators indicating that the activated sludge adapts to the new environment, and has certain guiding significance.
2)本发明方法检测方便。步骤简单易行,且测试设备自动化,例如使用西安绿标水环境科技有限公司提供的WBM系列污水处理智慧运行工作站,即可在无人操作的情况下自动化对待测试污泥进行测试。2) The method of the present invention is convenient for detection. The steps are simple and easy, and the test equipment is automated. For example, using the WBM series sewage treatment intelligent operation workstation provided by Xi'an Lvbiao Water Environment Technology Co., Ltd. can automatically test the sludge to be tested without human operation.
3)本发明所运用的参数并非水质这种非直观表征污水厂运行情况的指标,而是关注在污水处理的核心主体污泥上。污泥的自身状态才是真正影响污水厂运行的关键。而本发明所提供的以活性污泥内源呼吸比(OURe/OURt)关于时间t的变化趋势,作为活性污泥是否适应新环境的预示指标,更是在检测中简单直观易行。3) The parameters used in the present invention are not water quality, which is a non-intuitive indicator of the operation of the sewage plant, but focus on the core subject sludge of sewage treatment. The state of the sludge itself is the key that really affects the operation of the sewage plant. The change trend of the activated sludge endogenous respiration ratio (OURe/OURt) with respect to the time t provided by the present invention is used as a predictive indicator of whether the activated sludge adapts to the new environment, which is more simple and intuitive in detection.
附图说明Description of drawings
图1(a)(b)(c)(d)分别为西安市某污水厂活性污泥在降低DO和投加不同量的次氯酸钠条件下,所得到的(OURe/OURt)-t曲线。Figure 1(a)(b)(c)(d) are the (OURe/OURt)-t curves obtained under the conditions of reducing DO and adding different amounts of sodium hypochlorite to the activated sludge of a sewage plant in Xi'an respectively.
具体实施方式detailed description
下面通过附图及实施例对本发明做进一步的说明。The present invention will be further described below by means of drawings and embodiments.
本发明通过对活性污泥呼吸图谱分析来评估活性污泥是否适应新环境。The invention evaluates whether the activated sludge adapts to the new environment by analyzing the breath spectrum of the activated sludge.
本发明评估活性污泥是否适应新环境,包括以下步骤:The present invention assesses whether activated sludge adapts to the new environment, comprising the following steps:
1)取污水处理厂活性污泥300ml,用体积比1:3的比例添加自来水对泥进行稀释,将活性污泥样品通过搅拌、沉淀、去上清液、定容至活性污泥稀释液的1/2体积,然后用PBS缓冲溶液以0.2ml/s,进3s洗涤活性污泥一次,如此重复洗泥3次,之后添加自来水将活性污泥混合液定容至活性污泥稀释液的原体积,即1.2L,并且充分曝气2h,使活性污泥进入内源呼吸状态;1) Take 300ml of activated sludge from the sewage treatment plant, add tap water at a volume ratio of 1:3 to dilute the sludge, and stir the activated sludge sample, remove the supernatant, and dilute to the volume of the activated sludge dilution 1/2 volume, then wash the activated sludge once with PBS buffer solution at 0.2ml/s for 3s, repeat the mud washing for 3 times, then add tap water to dilute the activated sludge mixture to the original volume of the activated sludge dilution Volume, that is, 1.2L, and fully aerated for 2 hours, so that the activated sludge enters the endogenous respiration state;
缓冲溶液为以下组分混合液:The buffer solution is a mixture of the following components:
A:KH2PO4浓度为1.5~2.5mmol·L-1;A: KH2PO4 concentration is 1.5~2.5mmol·L -1 ;
B:Na2HPO4浓度为8~12mmol·L-1;B: The concentration of Na2HPO4 is 8-12mmol·L -1 ;
C:NaCl浓度为135~140mmol·L-1;C: NaCl concentration is 135-140 mmol·L -1 ;
D:KCl浓度为2.5~3.0mmol·L-1。D: KCl concentration is 2.5-3.0 mmol·L -1 .
2)进入内源呼吸状态后进行充分搅拌,获得第一段DO-t曲线,拟合得到其斜率,即为活性污泥内源呼吸速率OURe;2) After entering the state of endogenous respiration, fully stir to obtain the first section of DO-t curve, and get its slope by fitting, which is the endogenous respiration rate OURe of activated sludge;
3)在步骤2)结束后,污泥加入氮源按质量比每1g活性污泥中加入3~14mg含氮元素无机物(氯化铵或硫酸铵)或含氮元素有机物(尿素)。再加入碳源按质量比每1g活性污泥中加入200~700mg含碳元素有机物(乙酸钠,葡萄糖或淀粉)。充分曝气至溶氧DO达7mg/L以上,充分搅拌,最后得到另一段呼吸图谱DO-t曲线,拟合得到其斜率,即为总呼吸速率OURt;3) After step 2), nitrogen sources are added to the sludge. Add 3-14 mg nitrogen-containing inorganic matter (ammonium chloride or ammonium sulfate) or nitrogen-containing organic matter (urea) per 1 g of activated sludge by mass ratio. Then add carbon source and add 200-700 mg of carbon-containing organic matter (sodium acetate, glucose or starch) per 1 g of activated sludge according to the mass ratio. Fully aerate until the dissolved oxygen DO reaches 7mg/L or more, and then fully stir, and finally get another DO-t curve of the respiration map, and get its slope by fitting, which is the total respiration rate OURt;
呼吸图谱DO-t曲线满足条件:控制DO下降阶段持续时间t:5≤t≤25min和DO下降阶段变化范围:6.0≤DO≤4.0mg/L。获得呼吸图谱DO-t曲线过程的温度控制为8~45℃。The DO-t curve of the breath spectrum satisfies the conditions: control the duration of the DO decline phase t: 5≤t≤25min and the change range of the DO decline phase: 6.0≤DO≤4.0mg/L. The temperature control in the process of obtaining the DO-t curve of respiration atlas was 8-45°C.
投加氮源与碳源的质量比按照干活性污泥比例投加。The mass ratio of nitrogen source and carbon source is added according to the proportion of dry activated sludge.
4)重复步骤1)~3),分别得到随时间变化的内源呼吸速率OURe值与污泥总呼吸速率OURt值。4) Repeat steps 1) to 3) to obtain the time-varying endogenous respiration rate OURe and the total sludge respiration rate OURt respectively.
5)用OURe/OURt比值绘制关于时间t的曲线,根据OURe/OURt-t曲线的变化趋势,评估活性污泥是否适应新环境:①曲线呈先上升后平稳或先上升后下降趋势,预示活性污泥可以适应新环境;②曲线在环境条件改变一周后仍在上升,即呈持续上升趋势,说明内源呼吸速率OURe在增加,相应的功能性菌群的呼吸速率在减弱,预示活性污泥无法适应新环境,功能性菌群的活性将持续下降直至消失,最终解体;③曲线呈下降趋势,预示新环境有利于功能性菌群的生存,有利于活性污泥的生长。5) Use the OURe/OURt ratio to draw a curve about time t, and evaluate whether the activated sludge adapts to the new environment according to the changing trend of the OURe/OURt-t curve: ①The curve rises first and then stabilizes or first rises and then declines, indicating the activity Sludge can adapt to the new environment; ②The curve is still rising after changing the environmental conditions for a week, that is, showing a continuous upward trend, indicating that the endogenous respiration rate OURe is increasing, and the respiration rate of the corresponding functional flora is weakening, indicating that the activated sludge Unable to adapt to the new environment, the activity of functional flora will continue to decline until it disappears, and finally disintegrates; ③The curve shows a downward trend, indicating that the new environment is conducive to the survival of functional flora and the growth of activated sludge.
其中,功能性菌群包括自养菌和异养菌。Among them, the functional flora includes autotrophic bacteria and heterotrophic bacteria.
总呼吸速率OURt=内源呼吸速率OURe+以去除氨氮为主的自养菌的呼吸速率+去除BOD5的异养菌的呼吸速率。Total respiration rate OURt = endogenous respiration rate OURe + respiration rate of autotrophic bacteria mainly removing ammonia nitrogen + respiration rate of heterotrophic bacteria removing BOD 5 .
活性污泥可适应新环境包括:Activated sludge can be adapted to new environments including:
①活性污泥在正常溶氧转为低溶氧的环境;正常溶氧DO为2-4mg/L,所述低溶氧为DO<0.5mg/L;②活性污泥中加氯量≤8‰的环境。① Activated sludge is in an environment where normal dissolved oxygen changes to low dissolved oxygen; the normal dissolved oxygen DO is 2-4mg/L, and the low dissolved oxygen is DO<0.5mg/L; ② The amount of chlorine added to the activated sludge is ≤8 ‰environment of.
活性污泥无法适应新环境包括:活性污泥中加氯量为NaClO≥15‰的环境。The activated sludge cannot adapt to the new environment including: the environment where the amount of chlorine added to the activated sludge is NaClO ≥ 15‰.
下面通过具体实施例进一步说明本发明效果。The effect of the present invention is further illustrated below through specific examples.
1)对来源于西安市某污水处理厂A2/O工艺活性污泥进行取样;1) Sampling of A 2 /O process activated sludge from a sewage treatment plant in Xi'an;
2)选择西安绿标水环境科技有限公司提供的WBM450系列智慧运行工作站作为获得活性污泥呼吸图谱DO-t曲线的设备;2) Select the WBM450 series intelligent operation workstation provided by Xi'an Lvbiao Water Environment Technology Co., Ltd. as the equipment for obtaining the DO-t curve of the activated sludge respiration map;
3)取污水处理厂活性污泥300ml,并用体积比1:3的比例添加自来水稀释,将活性污泥样品通过搅拌、沉淀、去上清液、定容至活性污泥稀释液的1/2体积,用PBS缓冲溶液洗泥3次,之后添加自来水将活性污泥混合液定容至活性污泥稀释液的原体积,即1.2L,充分曝气2h,活性污泥进入内源呼吸状态;3) Take 300ml of activated sludge from the sewage treatment plant and dilute it with tap water at a ratio of 1:3 by volume. The activated sludge sample is stirred, precipitated, supernatant removed, and the volume is adjusted to 1/2 of the activated sludge dilution Volume, wash the mud with PBS buffer solution for 3 times, then add tap water to adjust the volume of the activated sludge mixture to the original volume of the activated sludge dilution, that is, 1.2L, fully aerate for 2 hours, and the activated sludge enters the endogenous respiration state;
4)在步骤3)污泥进入内源呼吸后,进行充分搅拌30min,获得呼吸图谱DO-t曲线,取溶氧为6-4mg/l的区间段,拟合得到其斜率,即得到内源呼吸速率OURe;4) After the sludge enters the endogenous respiration in step 3), fully stir for 30 minutes to obtain the DO-t curve of the respiration map, take the interval section of 6-4mg/l dissolved oxygen, and obtain the slope by fitting, that is, the endogenous respiration rate OURe;
表1给出了本实验中不同实施例工艺参数情况例举。Table 1 has provided the example of process parameter situation of different embodiments in this experiment.
表1不同实施例原料工艺参数Table 1 different embodiment raw material process parameters
5)在步骤4)结束后,加入氮源,使得氨氮浓度为50mg/l,加入碳源,使得容器内COD浓度为300mg/l,然后充分曝气至溶氧DO达7mg/L以上水平,之后进行充分搅拌30min,在温度下得到另一段呼吸图谱DO-t曲线,取溶氧为6-4mg/l的区间段,拟合得到其斜率,则会得到污泥加氮加碳后的总呼吸速率OURt;5) After step 4), add a nitrogen source so that the ammonia nitrogen concentration is 50 mg/l, add a carbon source so that the COD concentration in the container is 300 mg/l, and then fully aerate until the dissolved oxygen DO reaches a level above 7 mg/L, Afterwards, fully stir for 30 minutes, and obtain another section of DO-t curve of respiration spectrum at high temperature, take the section of dissolved oxygen of 6-4mg/l, and get its slope by fitting, then the total sludge after adding nitrogen and carbon will be obtained. respiration rate OURt;
6)在降低DO(<0.5mg/l)和投加不同量的次氯酸钠(2‰、8‰、15‰)的基础上重复以上步骤对污泥进行了连续监测,得到各自条件下随时间变化的内源呼吸速率OURe值与污泥总呼吸速率OUR值,用OURe/OURt比值绘制关于时间t的曲线。6) On the basis of reducing DO (<0.5mg/l) and adding different amounts of sodium hypochlorite (2‰, 8‰, 15‰), the above steps were repeated to continuously monitor the sludge, and the changes over time under the respective conditions were obtained. The endogenous respiration rate OURe value and the total sludge respiration rate OUR value are used to draw a curve about time t with the ratio of OURe/OURt.
实施例分析:Example analysis:
取西安市某污水处理厂A2/O工艺活性污泥,通过降低DO(其中正常DO为2-4mg/L),和投加不同量的次氯酸钠,测得各自条件下的呼吸图谱DO-t曲线。由获得的呼吸图谱DO-t曲线,得到各环境条件下的OURe/OURt曲线,如图1所示。图1(a)是反应器在正常溶氧下运行一段时间,后转为低溶氧(<0.5mg/L)环境,一段时间后再改变其环境,将其恢复至正常溶氧同时加入15‰的NaClO,活性污泥OURe/OURt-t的曲线变化图;而图1(b)是重启实验后,活性污泥在正常溶氧下生存一段时间,后转为低溶氧(<0.5mg/L)下生存的OURe/OURt-t曲线变化图;图1(c)(d)为同时间的两个试验,图中矩形折线表示NaClO的投加过程,在加入2‰后一段时间加入8‰而后停止投加NaClO的活性污泥OURe/OURt-t曲线变化图。发现在投加不同量的次氯酸钠后,高氯量(15mg/g SS)下污泥发生解体,即污泥无法适应新环境;而在低氯量(2‰、8‰)下污泥则不会解体,即污泥可以适应新环境,在降低DO的试验中,污泥未发生解体,即污泥可以适应新环境。对比其各自条件下的OURe/OURt-t曲线,显而易见:①曲线在低溶氧或低氯量环境条件下呈先上升后平稳或先上升后下降趋势,在加入2‰后一段时间再加入8‰NaClO后停止加氯,曲线也呈先上升后下降趋势,预示活性污泥可以适应新环境,其短暂性上升是因为微生物在进行适应性调整;②在15‰的高氯量环境条件下曲线呈持续上升趋势,预示活性污泥无法适应新环境,功能性菌群的活性持续下降直至消失,最终解体。③曲线呈直接下降趋势,预示新环境有利于功能性菌群的生存,有利于活性污泥的生长。这与实验获得的OURe/OURt-t曲线变化趋势所反映的活性污泥情况一致,完全吻合。也就是说,通过取污水处理厂活性污泥获得呼吸图谱DO-t曲线,根据其得到OURe/OURt-t曲线,通过此曲线的变化趋势,便可准确评估活性污泥是否适应新环境。Take the A 2 /O process activated sludge from a sewage treatment plant in Xi'an City, by reducing DO (the normal DO is 2-4mg/L), and adding different amounts of sodium hypochlorite, and measure the respiratory spectrum DO-t under the respective conditions curve. From the DO-t curve of the obtained respiration spectrum, the OURe/OURt curve under various environmental conditions is obtained, as shown in Figure 1. Figure 1(a) shows that the reactor operates under normal dissolved oxygen for a period of time, then turns to a low dissolved oxygen (<0.5mg/L) environment, changes its environment after a period of time, restores it to normal dissolved oxygen and adds 15 ‰ NaClO, the curve change diagram of activated sludge OURe/OURt-t; and Figure 1(b) is after restarting the experiment, the activated sludge survived under normal dissolved oxygen for a period of time, and then turned to low dissolved oxygen (<0.5mg /L) of OURe/OURt-t curve changes for survival; Figure 1(c)(d) shows two experiments at the same time, and the rectangular broken line in the figure indicates the process of adding NaClO, which was added after adding 2‰ 8‰ and then stop adding NaClO activated sludge OURe/OURt-t curve diagram. It was found that after adding different amounts of sodium hypochlorite, the sludge disintegrated under high chlorine content (15mg/g SS), that is, the sludge could not adapt to the new environment; while under low chlorine content (2‰, 8‰), the sludge did not It will disintegrate, that is, the sludge can adapt to the new environment. In the experiment of reducing DO, the sludge did not disintegrate, that is, the sludge can adapt to the new environment. Comparing the OURe/OURt-t curves under their respective conditions, it is obvious: ①The curves show a trend of rising first and then stabilizing or first rising and then falling under the environment conditions of low dissolved oxygen or low chlorine content. After adding 2‰, add 8 Chlorination was stopped after ‰NaClO, and the curve also showed a trend of rising first and then falling, indicating that the activated sludge can adapt to the new environment, and the short-term rise is due to the adaptive adjustment of microorganisms; The continuous upward trend indicates that the activated sludge cannot adapt to the new environment, and the activity of the functional flora continues to decline until it disappears and finally disintegrates. ③The curve shows a direct downward trend, indicating that the new environment is conducive to the survival of functional flora and the growth of activated sludge. This is consistent with the activated sludge situation reflected by the changing trend of the OURe/OURt-t curve obtained from the experiment. That is to say, the DO-t curve of the respiration spectrum is obtained by taking the activated sludge from the sewage treatment plant, and the OURe/OURt-t curve is obtained according to it. Through the change trend of this curve, it is possible to accurately evaluate whether the activated sludge can adapt to the new environment.
综上可见,本发明是一种基于呼吸图谱评估活性污泥是否适应新环境的方法。In summary, the present invention is a method for assessing whether activated sludge adapts to a new environment based on respiration patterns.
以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,采用调整DO量和氯的投加量的方式,控制DO-t曲线DO下降阶段持续时间t、DO下降阶段的变化范围,从而得到呼吸图谱DO-t曲线,继而得到OURe/OURt-t曲线过程中,在此仅仅给出了较佳的实施例,除此,本发明权利还包括其他环境因素的改变,如温度、pH等。任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,可轻易想到的变化或替换,都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, and the DO amount and chlorine dosage are adjusted to control the duration of the DO-t curve DO decline stage. t, the variation range of the DO descending stage, thereby obtaining the DO-t curve of the respiratory spectrum, and then obtaining the process of the OURe/OURt-t curve, only a preferred embodiment has been given here, and in addition, the rights of the present invention also include other Changes in environmental factors such as temperature, pH, etc. Any changes or substitutions that can be easily conceived by any person skilled in the art within the technical scope disclosed in the present invention shall fall within the protection scope of the present invention.
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