CN108866204A - A kind of selection of elongated mirror carp fast-growth strain - Google Patents
A kind of selection of elongated mirror carp fast-growth strain Download PDFInfo
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- CN108866204A CN108866204A CN201810788002.XA CN201810788002A CN108866204A CN 108866204 A CN108866204 A CN 108866204A CN 201810788002 A CN201810788002 A CN 201810788002A CN 108866204 A CN108866204 A CN 108866204A
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Abstract
The selection of the elongated mirror carp fast-growth strain of the present invention, comprehensive utilization genetic marker information, individual amount trait information, genetic distance etc. match parental combination by linkage analysis, and production performance and accuracy can be improved, avoid inbred, shorten breeding process.Selection：Genetic distance is between 0.5~0.7 between different reproductive pedigree, parent individual and filial generation can be enriched with the individual of 6 or more advantage allele for selection, carries out family or breeding is completed in the breeding of small group combo, filial generation sexal maturity.The method of the present invention is able to maintain that the higher genetic diversity of mirror carp, improves mirror carp production performance, accelerates the breeding process of mirror carp.
Description
Technical field
The present invention relates to a kind of selections of mirror carp.
Background technique
Mirror carp (Cyprinus carpio L.) is from after introducing China, by the systematic breeding and selection cross of decades,
Formd multiple kinds, had the characteristics that growth is fast, quality is excellent, meat is thick and preferably process, become carp main breed variety it
One.But traditional breeding method is mainly according to Selection parent with properties and characteristics, and leading to that breeding accuracy is poor, the breeding period is grown etc. asks
Topic.
Summary of the invention
The present invention provides a kind of selection of genetic marker auxiliary, this method comprehensive utilization heredity mark for elongated mirror carp
Note information, individual amount trait information, genetic distance etc. match parental combination by linkage analysis, can be improved production performance and
Accuracy avoids inbred, shortens breeding process.
The selection of the elongated mirror carp fast-growth strain of the present invention carries out according to the following steps：
Genetic distance is between 0.5~0.7 between different reproductive pedigree, parent individual and filial generation can be enriched with 6 for selection
The individual of a above advantage allele, carries out family or breeding is completed in the breeding of small group combo, filial generation sexal maturity.
Wherein, advantage allele is chosen from following 20 microsatellite markers：
The method of the present invention is able to maintain that the higher genetic diversity of mirror carp, improves mirror carp production performance, accelerates the choosing of mirror carp
Educate process.
Specific embodiment
The technical solution of the present invention is not limited to the following list, further includes between each specific embodiment
Any combination.
Specific embodiment one：The selection of the elongated mirror carp fast-growth strain of present embodiment, it is characterised in that should
Selection carries out according to the following steps：
Genetic distance is between 0.5~0.7 between different reproductive pedigree, parent individual and filial generation can be enriched with 6 for selection
The individual of a above advantage allele, carries out family or breeding is completed in the breeding of small group combo, filial generation sexal maturity；
Wherein, advantage allele in table 1 in 20 microsatellite markers from choosing, and objects advantages genotype is also such as table
Shown in 1：
Table 1
The fragment length and genotype that individual without advantageous allele amplifies are not inconsistent with table 1.
Specific embodiment two：The difference of present embodiment and specific embodiment one is：With genetic analysis software meter
It calculates and genetic distance and draws chadogram between individual.Other steps and parameter are identical as embodiment one.
Genetic analysis software described in present embodiment is " the molecular breeding software 1.0 based on genetic background ".
Specific embodiment three：The difference of present embodiment and specific embodiment one or two is：In each microsatellite
5 ' end label red fluorescences, green fluorescence, yellow fluorescence or the blue-fluorescence of the forward primer of label.Other steps and parameter with
Embodiment one or two is identical.
Specific embodiment four：The difference of present embodiment and specific embodiment one, two or three is：Amplification advantage etc.
Pcr amplification reaction system is 15 μ L, Tris-Cl, 50mmol/ including final concentration 10mmol/L pH value 8.3 during the gene of position
L KCl、1.5mmol/L MgCl2, 200 μm of ol/L dNTP, 0.3 μm of ol/L micro-satellite primers, 1U Taq archaeal dna polymerase,
The deionized water of 100ng DNA profiling and surplus；PCR response procedures are 94 DEG C of initial denaturation 3min；94 DEG C of denaturation 30s, 56 DEG C it is multiple
Property 30s, 72 DEG C of extension 30s, 25 circulation；Last 72 DEG C of extensions 5min.Other steps and parameter and embodiment one, two or three
It is identical.
Present embodiment PCR carries out capillary gel electrophoresis with 3730XL genetic analyzer after reaction, utilizes
GeneMappre V4.1 software carries out image collection and data analysis, obtains genotype data.
Embodiment 1
The selection of elongated mirror carp fast-growth strain：
Field lens carp F is tested from Heilungkiang aquatic products research institute Hulan aquatic products2It is fast that growth is picked out in (2 age), figure is partially long, and
Only at the parental population of elongated mirror carp fast-growth strain breeding, weight is the squamose 350 tail group of individuals in back two sides
1000g~1500g, a length of 30cm~36cm of body, the long body ratios of body are 2.60~3.00, are implanted into electronic marker.Acquire parent
The isozyme of group extracts simultaneously purified genomic dna with tissue extraction kit, then measures purity with ultraviolet specrophotometer
And concentration, it is diluted to 100ng/ μ L.The microsatellite marker primer pair amplifies genomic DNA shown in table 1, in each microsatellite marker
5 ' ends of forward primer mark red (PET), green (VIC), yellow (NED) or blue (6FAM) fluorescence；Establish 15 μ LPCR
Amplification reaction system, Tris-Cl, 50mmol/L KCl, 1.5mmol/L of the system by final concentration 10mmol/L pH value 8.3
MgCl2, 200 μm of ol/L dNTP, 0.3 μm of ol/L micro-satellite primers, 1U Taq archaeal dna polymerase, 100ng DNA profiling and surpluses
Deionized water composition；It is carried out amplification reaction using 9700 types (ABI) PCR instrument, PCR response procedures are 94 DEG C of initial denaturation 3min；
94 DEG C of denaturation 30s, 56 DEG C of renaturation 30s, 72 DEG C of extension 30s, 25 circulations；Last 72 DEG C of extensions 5min.After reaction, every color
Fluorescent marker takes 0.7 μ L fluorescence PCR products (can arbitrarily mix four color fluorescent markers), the Hi-Di of 5.9 μ LTMFormamide and 0.1
μ LLIZ-500 is prepared into electrophoresis aggregate sample.Aggregate sample is placed in PCR instrument in 95 DEG C of denaturation 5min, cooled on ice is immediately placed on
5min；Capillary gel electrophoresis is carried out on 3730XL genetic analyzer (ABI), utilizes GeneMappre after electrophoresis
V4.1 software carries out image collection and data analysis, obtains genotype data.Using genetic analysis software " based on genetic background
Genetic distance between the analysis individual of molecular breeding software 1.0 ", and draw cluster numbers between individual.By 350 individuals according to hereditary difference
Be divided into different branches, selection is located at different branches and genetic distance between 0.5~0.7, and 6 or more sites contain it is excellent
Gesture allele and the male and female individual progress family combo for meeting filial generation objects advantages genotype.Select genetic distance be located at 0.5~
Between 0.7, comprising 5 and the parent of following advantage allele as a control group.The Offspring Growth speed that the method for the present invention obtains
Degree improves 8.6% than control group.
Claims (7)
1. a kind of selection of elongated mirror carp fast-growth strain, it is characterised in that the selection carries out according to the following steps：
Selection between different reproductive pedigree, parent individual genetic distance between 0.5~0.7 and filial generation can be enriched with 6 with
The individual of upper advantage allele, carries out family or breeding is completed in the breeding of small group combo, filial generation sexal maturity.
2. a kind of selection of elongated mirror carp fast-growth strain according to claim 1, it is characterised in that with heredity
Analysis software genetic distance and draws chadogram between calculating individual.
3. a kind of selection of elongated mirror carp fast-growth strain according to claim 1, it is characterised in that advantage etc.
Position gene is chosen from following 20 microsatellite markers：
4. a kind of selection of elongated mirror carp fast-growth strain according to claim 3, it is characterised in that each
5 ' end label red fluorescences, green fluorescence, yellow fluorescence or the blue-fluorescence of the forward primer of microsatellite marker.
5. a kind of selection of elongated mirror carp fast-growth strain according to claim 3, it is characterised in that amplification is excellent
Pcr amplification reaction system is 15 μ L during gesture allele, Tris-Cl including final concentration 10mmol/L pH value 8.3,
50mmol/L KCl、1.5mmol/L MgCl2, 200 μm of ol/L dNTP, 0.3 μm of ol/L micro-satellite primers, 1U Taq DNA it is poly-
The deionized water of synthase, 100ng DNA profiling and surplus；PCR response procedures are 94 DEG C of initial denaturation 3min；94 DEG C of denaturation 30s, 56
DEG C renaturation 30s, 72 DEG C of extension 30s, 25 circulations；Last 72 DEG C of extensions 5min.
6. a kind of selection of elongated mirror carp fast-growth strain according to claim 5, it is characterised in that PCR reaction
After with 3730XL genetic analyzer carry out capillary gel electrophoresis, utilize GeneMappre V4.1 software to carry out image receipts
Collection and data analysis, obtain genotype data.
7. a kind of selection of elongated mirror carp fast-growth strain according to claim 2, it is characterised in that the something lost
Passing analysis software is the molecular breeding software 1.0 based on genetic background.
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| CN201810719997 | 2018-07-03 | ||
| CN2018107199974 | 2018-07-03 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631174A (en) * | 2020-05-29 | 2020-09-08 | 广西壮族自治区水产科学研究院 | Breeding method of Cyprinus carpiod |
| CN111793699A (en) * | 2020-08-13 | 2020-10-20 | 中国水产科学研究院黑龙江水产研究所 | Efficient matching and breeding method for procypris merus |
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| CN101120667A (en) * | 2007-09-26 | 2008-02-13 | 中国水产科学研究院黑龙江水产研究所 | Method for optimizing variety of carp |
| CN101403006A (en) * | 2008-11-27 | 2009-04-08 | 中国水产科学研究院黑龙江水产研究所 | Screening method for species group with heterosis of germany mirror carp |
| CN102586451A (en) * | 2012-03-09 | 2012-07-18 | 中国水产科学研究院淡水渔业研究中心 | Method for matching and breeding jian carps |
| CN106399480A (en) * | 2016-08-30 | 2017-02-15 | 上海海洋大学 | Micro-satellite genetic fingerprints of megalobrama amblycephala sperm induced grass carp meiosis gynogenetic offspring |
| CN111793699A (en) * | 2020-08-13 | 2020-10-20 | 中国水产科学研究院黑龙江水产研究所 | Efficient matching and breeding method for procypris merus |
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2018
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| CN101120667A (en) * | 2007-09-26 | 2008-02-13 | 中国水产科学研究院黑龙江水产研究所 | Method for optimizing variety of carp |
| CN101403006A (en) * | 2008-11-27 | 2009-04-08 | 中国水产科学研究院黑龙江水产研究所 | Screening method for species group with heterosis of germany mirror carp |
| CN102586451A (en) * | 2012-03-09 | 2012-07-18 | 中国水产科学研究院淡水渔业研究中心 | Method for matching and breeding jian carps |
| CN106399480A (en) * | 2016-08-30 | 2017-02-15 | 上海海洋大学 | Micro-satellite genetic fingerprints of megalobrama amblycephala sperm induced grass carp meiosis gynogenetic offspring |
| CN111793699A (en) * | 2020-08-13 | 2020-10-20 | 中国水产科学研究院黑龙江水产研究所 | Efficient matching and breeding method for procypris merus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631174A (en) * | 2020-05-29 | 2020-09-08 | 广西壮族自治区水产科学研究院 | Breeding method of Cyprinus carpiod |
| CN111793699A (en) * | 2020-08-13 | 2020-10-20 | 中国水产科学研究院黑龙江水产研究所 | Efficient matching and breeding method for procypris merus |
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