CN108860731A - A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen - Google Patents

A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen Download PDF

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Publication number
CN108860731A
CN108860731A CN201810804062.6A CN201810804062A CN108860731A CN 108860731 A CN108860731 A CN 108860731A CN 201810804062 A CN201810804062 A CN 201810804062A CN 108860731 A CN108860731 A CN 108860731A
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CN
China
Prior art keywords
plant leaf
leaf specimen
specimen
packaging bag
vacuum packaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810804062.6A
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Chinese (zh)
Inventor
马琼
周志
张弛
方庆
董静洲
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Hubei University for Nationalities
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Hubei University for Nationalities
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Filing date
Publication date
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Priority to CN201810804062.6A priority Critical patent/CN108860731A/en
Publication of CN108860731A publication Critical patent/CN108860731A/en
Pending legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65BMACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B25/00Packaging other articles presenting special problems
    • B65B25/02Packaging agricultural or horticultural products
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65BMACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B31/00Packaging articles or materials under special atmospheric or gaseous conditions; Adding propellants to aerosol containers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65BMACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B31/00Packaging articles or materials under special atmospheric or gaseous conditions; Adding propellants to aerosol containers
    • B65B31/04Evacuating, pressurising or gasifying filled containers or wrappers by means of nozzles through which air or other gas, e.g. an inert gas, is withdrawn or supplied
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65BMACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B63/00Auxiliary devices, not otherwise provided for, for operating on articles or materials to be packaged
    • B65B63/08Auxiliary devices, not otherwise provided for, for operating on articles or materials to be packaged for heating or cooling articles or materials to facilitate packaging

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of NEW TYPE OF COMPOSITEs of plant leaf specimen to handle packing method, the vacuum pack with nitrogen step including plant leaf specimen disinfection freezing processing step and plant leaf specimen, and operation of the present invention is convenient;It should not make moist, rot, go mouldy, will not be damaged by insects, the preservation time is long;It does not need often to replace camphor ball and other drugs in preservation, no bad smell is more environmentally friendly.

Description

A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen
Technical field
The present invention relates to plant specimen fields, and in particular to a kind of NEW TYPE OF COMPOSITE processing packaging side of plant leaf specimen Method.Applied to plant specimen preservation.
Background technique
Traditional processing packing method is to be dipped in 0.3~1% mercuric chloride after pressing dry plant specimen with blotting paper (HgCl2) sterilize in alcoholic solution, it dries, paper of appearing on the stage, is packed into specimen bag, the preservation of 20g camphor ball is put into specimen bag.Tradition side The plant leaf specimen of method processing packaging is easy dampness, rotten, mildew or damages by worms, and storage life is not grown, and additionally needs to regularly replace Camphor ball, drug volatilization bad smell in specimen bag etc..
Summary of the invention
It is an object of the invention to overcome prior art defect, a kind of NEW TYPE OF COMPOSITE processing packet of plant leaf specimen is provided Dress method.
The invention is realized by the following technical scheme:
A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen, includes the following steps:
Step 1, plant leaf specimen sterilize freezing processing, specially:
Compaction drying arrangement is carried out to plant leaf specimen, plant leaf specimen is taken out, plant leaf specimen is dipped in dense Degree is dried, the plant leaf specimen dried is appeared on the stage paper to sterilize 2~5min in 0.3~1% mercuric chloride alcoholic solution, platform Paper upper left corner patch record label, then plant leaf specimen is put into vacuum packaging bag transparent, with a thickness of 8~13 suitable sizes, Then the vacuum packaging bag for being put into plant leaf specimen is placed in -20 to -80 DEG C of refrigerators and is placed 7~10 days.From refrigerator The vacuum packaging bag for being put into plant leaf specimen is taken out, plant leaf specimen is placed in ventilation together with vacuum packaging bag and does Dry place air-dries, and places into camphor ball in vacuum packaging bag.
The vacuum pack with nitrogen of step 2, plant leaf specimen, specially:
To step 1, treated that the vacuum packaging bag of plant leaf specimen is housed, and utilizes vacuum nitrogen filling packing machine to extract empty Gas is simultaneously sealed after being filled with nitrogen so that in vacuum packaging bag the percent by volume of oxygen 1% hereinafter, air pressure be 0.01~ 0.05Mpa。
Above-mentioned routine compaction drying arrangement includes the following steps:
Multi-layer plant herbarium is laid between two clamping plates, between plant leaf specimen and clamping plate and vegetable wax leaf It is equipped with blotting paper between sample and plant leaf specimen, clips two clamping plates with specimen holder, is bound with rope, is placed in logical It is dry at wind, and in being changed in 1 day paper 2~3 times.
The present invention has the following advantages that compared with the existing technology:
1, easy to operate;
2, it should not make moist, rot, go mouldy, will not be damaged by insects, the preservation time is long;
3, it does not need often to replace camphor ball and other drugs in preservation, no bad smell is more environmentally friendly.
Specific embodiment
For the ease of those of ordinary skill in the art understand and implement the present invention, below with reference to embodiment to the present invention make into The detailed description of one step, it should be understood that implementation example described herein is merely to illustrate and explain the present invention, and is not used to limit The fixed present invention.
Embodiment 1:
A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen, includes the following steps:
Step 1, plant leaf specimen sterilize freezing processing
Compaction drying arrangement is carried out to plant leaf specimen, plant leaf specimen is taken out, plant leaf specimen is dipped in dense Mercuric chloride (the HgCl that degree is 0.3~1%2) 2~5min is sterilized in alcoholic solution, it dries.It will be on the plant leaf specimen that dried Platform paper, platform paper upper left corner patch record label, then plant leaf specimen is put into vacuum transparent, with a thickness of 8~13 suitable sizes Then the vacuum packaging bag for being put into plant leaf specimen is placed in -20 to -80 DEG C of refrigerators and places 7~10 days by packaging bag.It is cold Freeze temperature to have to be suitable for that cryogenic temperature minute quantity moisture contained by plant leaf specimen at -20 DEG C or less all freezes, it can Make to remain in the biological cell on plant leaf specimen or brood body freeze thawing death.It is taken out from refrigerator and has been put into vegetable wax leaf mark This vacuum packaging bag, plant leaf specimen are placed at aeration-drying together with vacuum packaging bag and air-dry, and place into 20g camphor tree Brain ball is in vacuum packaging bag.
Above-mentioned routine compaction drying arrangement includes the following steps:
When compacting, multi-layer plant herbarium is laid between two clamping plates, between plant leaf specimen and clamping plate, and Blotting paper is equipped between plant leaf specimen and plant leaf specimen (according to aqueous how many determining suctions of plant leaf specimen The number of plies of water paper), water suction paper size should be identical as clamping plate, pays attention to that arrangement keeps plant leaf specimen smooth, for be not easy to press dry Succulent plant sample is first scalded with boiled water after death re-compacted.Two clamping plates are clipped with specimen holder, are bound with rope, ventilation is placed in Locate drying, and in changing paper 2-3 times in 1 day, should be noted that when changing paper not make plant leaf specimen fold.
The vacuum pack with nitrogen of step 2, plant leaf specimen
Using small food vacuum nitrogen filling packing machine, to step 1, treated that the vacuum packaging of plant leaf specimen is housed Bag carries out vacuum pack with nitrogen.The air in vacuum packaging bag is extracted, condition is vacuum-packed:Pumping can not make vacuum packaging bag Interior absolute anaerobic, it is desirable that the percent by volume of the oxygen in vacuum packaging bag is 1% hereinafter, being suppressed microorganism and worm's ovum And stop breeding, if micro- life of the percent by volume of the oxygen in vacuum packaging bag on 1.5% or more, plant leaf specimen Object is the same as growth and breeding in air;The inert nitrogen gas being filled in vacuum packaging bag is able to maintain plant leaf specimen original Some appearances will not be such that plant leaf specimen deforms, and bag face is wrinkle resistant.The nitrogen pressure of small food vacuum nitrogen filling packing machine is set 0.3~0.5MPa of power, 25~50s of air extraction time, nitrogen 2~5s of inflationtime heat the temperature and time of sealing according to packet Depending on pack thickness, it is desirable that sealing is air tight, heats 8~15mm of seal widths.It is required that in packaging bag after vacuum pack with nitrogen Oxygen is 1% hereinafter, 0.01~0.05Mpa of pressure.Vacuum packaging bag has moisture-proof, oxygen barrier, antistatic, vacuum-pumping spy Property, thickness is had to, and to be suitable for that vacuum packaging bag is too thin be easily damaged when heating sealing, and pack does not have vacuum-packed anti- Protect effect;It is too thick to be easy to happen cracking, gas leakage, influence the storage life of plant leaf specimen.
Embodiment 2:
A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen, includes the following steps:
Step 1, plant leaf specimen sterilize freezing processing
Compaction drying is carried out to plant leaf specimen and arranges taking-up plant leaf specimen, plant leaf specimen is dipped in concentration For 0.8% mercuric chloride (HgCl2) 3min is sterilized in alcoholic solution, it dries, the plant leaf specimen dried is appeared on the stage paper, platform paper Upper left corner patch record label.Again by plant leaf specimen be put into it is transparent, with a thickness of 10 suitable size vacuum packaging bags, then set It is placed 9 days in -70 DEG C of refrigerators.The vacuum packaging bag for being put into plant leaf specimen, plant leaf specimen are taken out from refrigerator It is placed at aeration-drying and air-dries together with vacuum packaging bag, place into 20g camphor ball in vacuum packaging bag.
Above-mentioned routine compaction drying arrangement includes the following steps:
When compacting, multi-layer plant herbarium is laid between two clamping plates, between plant leaf specimen and clamping plate, and Blotting paper is equipped between plant leaf specimen and plant leaf specimen (according to aqueous how many determining suctions of plant leaf specimen The number of plies of water paper), water suction paper size should be identical as clamping plate, pays attention to that arrangement keeps plant leaf specimen smooth, for be not easy to press dry Succulent plant sample is first scalded with boiled water after death re-compacted.Two clamping plates are clipped with specimen holder, are bound with rope, ventilation is placed in Locate drying, and in changing paper 2-3 times in 1 day, should be noted that when changing paper not make plant leaf specimen fold.
The vacuum pack with nitrogen of step 2, plant leaf specimen
Using small food vacuum nitrogen filling packing machine, to step 1, treated that the vacuum packaging of plant leaf specimen is housed Bag carries out vacuum pack with nitrogen.The air in vacuum packaging bag is extracted, condition is vacuum-packed:Pumping can not make vacuum packaging bag Interior absolute anaerobic, it is desirable that oxygen in vacuum packaging bag 1% hereinafter, be suppressed microorganism and worm's ovum and stop breeding, If microorganism of the oxygen in vacuum packaging bag on 1.5% or more, plant leaf specimen is numerous with equally growing in air It grows;The inert nitrogen gas being filled in pack is able to maintain the original appearance of plant leaf specimen, will not make plant leaf specimen Deformation, bag face are wrinkle resistant.The nitrogen pressure 0.4MPa of small food vacuum nitrogen filling packing machine, air extraction time 40s, nitrogen are set Gas inflationtime 3s, depending on heating the temperature and time of sealing according to packaging bag thickness, it is desirable that sealing is air tight, heats seal widths 8~15mm.It is required that the percent by volume of oxygen is 0.7% in vacuum packaging bag after vacuum pack with nitrogen, pressure 0.03Mpa.
Using the processing method and traditional processing packing method of the present embodiment 2 save respectively xylophyta, herbaceous plant, Pteridophyte, gymnosperm and each 300 parts of angiosperm save in the sample coarctation cabinet being put into sample storeroom, close close Collect cabinet cabinet door, fastens the door and window and curtain of sample storeroom.Sample storage room temperature maintains 4~20 DEG C, relative humidity 55 Between~65%, while annual spring and autumn is fumigated sample storeroom 2 times with drug.After plant leaf specimen saves 5 years, bacterium is counted The disease rate (table 1) of disease, fungal disease and insects disease.
The disease rate comparison sheet of table 1 present invention and the plant leaf specimen of conventional packaging methods packaging
As known from Table 1, using plant leaf specimen bacteriosis, fungal disease and the worm of NEW TYPE OF COMPOSITE processing packing method Class disease is significantly lower than conventional packaging methods, and therefore, plant leaf specimen can be effectively reduced in NEW TYPE OF COMPOSITE processing packing method It rots, go mouldy, damage by worms, make moist, hence it is evident that extend plant leaf specimen storage life.In addition, NEW TYPE OF COMPOSITE processing packing method is kept away Camphor ball, drug volatilization bad smell in the drawbacks of having exempted from conventional process packing method, such as periodic replacement specimen bag, harm Physical and mental health brings inconvenience and difficult to management.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention The technical staff in domain can make various modifications or additions to described specific embodiment or replace by a similar method In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.

Claims (2)

1. a kind of NEW TYPE OF COMPOSITE of plant leaf specimen handles packing method, which is characterized in that include the following steps:
Step 1, plant leaf specimen sterilize freezing processing, specially:
Compaction drying arrangement is carried out to plant leaf specimen, takes out plant leaf specimen, plant leaf specimen, which is dipped in concentration, is 2~5 min are sterilized in the mercuric chloride alcoholic solution of 0.3~1 %, are dried, and the plant leaf specimen dried is appeared on the stage paper, a platform paper left side Upper angle patch record label, then plant leaf specimen is put into vacuum packaging bag transparent, with a thickness of 8~13 suitable sizes, then The vacuum packaging bag for being put into plant leaf specimen is placed in -20 to -80 DEG C of refrigerators and is placed 7~10 days, is taken from refrigerator It is put into the vacuum packaging bag of plant leaf specimen out, plant leaf specimen is placed in aeration-drying together with vacuum packaging bag Place air-dries, and places into camphor ball in vacuum packaging bag,
The vacuum pack with nitrogen of step 2, plant leaf specimen, specially:
To step 1 treated be equipped with plant leaf specimen vacuum packaging bag, using vacuum nitrogen filling packing machine extract air simultaneously It is sealed after being filled with nitrogen, so that the percent by volume of oxygen is in 1 % hereinafter, air pressure is 0.01~0.05 in vacuum packaging bag Mpa。
2. a kind of NEW TYPE OF COMPOSITE of plant leaf specimen according to claim 1 handles packing method, which is characterized in that on Conventional compaction drying arrangement is stated to include the following steps:
Multi-layer plant herbarium is laid between two clamping plates, between plant leaf specimen and clamping plate and plant leaf specimen It is equipped with blotting paper between plant leaf specimen, clips two clamping plates with specimen holder, is bound with rope, be placed in ventilation It is dry, and in being changed in 1 day paper 2~3 times.
CN201810804062.6A 2018-07-20 2018-07-20 A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen Pending CN108860731A (en)

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Application Number Priority Date Filing Date Title
CN201810804062.6A CN108860731A (en) 2018-07-20 2018-07-20 A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810804062.6A CN108860731A (en) 2018-07-20 2018-07-20 A kind of NEW TYPE OF COMPOSITE processing packing method of plant leaf specimen

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Publication Number Publication Date
CN108860731A true CN108860731A (en) 2018-11-23

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1062262A (en) * 1990-12-13 1992-07-01 中国医学科学院药用植物资源开发研究所 Fast pressing approach for obtaining plant herbarium with original colours
JP2001071695A (en) * 1999-07-02 2001-03-21 Chizuko Okazaki Quick drying method for raw sample and preserving method for dried body
CN1384476A (en) * 2002-04-11 2002-12-11 新疆奥斯曼生物科技有限公司 Snow lotus sample making process
CN1623805A (en) * 2004-08-14 2005-06-08 延边朝鲜族自治州民族医药研究所 Method for making plant leaf specimen
CN102499234A (en) * 2011-10-27 2012-06-20 云南省烟草农业科学研究院 Manufacturing method for liquid film closed type plant primary-color herbarium specimens
CN103210905A (en) * 2013-02-21 2013-07-24 江苏省中国科学院植物研究所 Manufacturing method of plant leaf specimen teaching aid
CN104186461A (en) * 2014-08-19 2014-12-10 中华全国供销合作总社昆明食用菌研究所 Preparation method of edible fungus specimen
CN106508893A (en) * 2016-09-21 2017-03-22 中国科学院武汉植物园 Method for pressing dehydration of plant herbarium specimens
CN106561638A (en) * 2016-11-09 2017-04-19 沈阳农业大学 Plant specimen plastic sealing method

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1062262A (en) * 1990-12-13 1992-07-01 中国医学科学院药用植物资源开发研究所 Fast pressing approach for obtaining plant herbarium with original colours
JP2001071695A (en) * 1999-07-02 2001-03-21 Chizuko Okazaki Quick drying method for raw sample and preserving method for dried body
CN1384476A (en) * 2002-04-11 2002-12-11 新疆奥斯曼生物科技有限公司 Snow lotus sample making process
CN1623805A (en) * 2004-08-14 2005-06-08 延边朝鲜族自治州民族医药研究所 Method for making plant leaf specimen
CN102499234A (en) * 2011-10-27 2012-06-20 云南省烟草农业科学研究院 Manufacturing method for liquid film closed type plant primary-color herbarium specimens
CN103210905A (en) * 2013-02-21 2013-07-24 江苏省中国科学院植物研究所 Manufacturing method of plant leaf specimen teaching aid
CN104186461A (en) * 2014-08-19 2014-12-10 中华全国供销合作总社昆明食用菌研究所 Preparation method of edible fungus specimen
CN106508893A (en) * 2016-09-21 2017-03-22 中国科学院武汉植物园 Method for pressing dehydration of plant herbarium specimens
CN106561638A (en) * 2016-11-09 2017-04-19 沈阳农业大学 Plant specimen plastic sealing method

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Application publication date: 20181123