CN108855257A - A kind of preparation method of papery micro-fluidic chip - Google Patents
A kind of preparation method of papery micro-fluidic chip Download PDFInfo
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- CN108855257A CN108855257A CN201810385689.2A CN201810385689A CN108855257A CN 108855257 A CN108855257 A CN 108855257A CN 201810385689 A CN201810385689 A CN 201810385689A CN 108855257 A CN108855257 A CN 108855257A
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical group C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 11
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
Abstract
The invention discloses a kind of preparation methods of papery micro-fluidic chip, include the following steps：Pattern template is prepared by processing the pattern with microchannel in template；Liquid barrier is coated in the pattern, makes to form the barrier seal with the pattern on the pattern template；Analysis paper is bonded with the barrier seal, barrier is made to penetrate into the tow sides on the analysis paper same position；Heating or UV irradiation are carried out to the analysis paper that tow sides are impregnated with barrier, solidifies barrier in the analysis paper, forms papery micro-fluidic chip.Preparation method provided by the invention is easy to operate, only pass through cutting pattern template, the requirement for meeting papery fluid channel can be formed without instrument and equipment, microchannel obtained effectively prevents the seepage flow of liquid, provides a kind of method efficiently, inexpensive and practical for the micro-fluidic production of papery.
Description
Technical field
The invention belongs to chip technology fields, and in particular to a kind of preparation method of papery micro-fluidic chip.
Background technique
Micro-fluidic (MicrofIuidics) is a kind of scientific skill using microchannel accurate manipulation and control minute yardstick fluid
Art.The high sensitivity of analyte can be achieved, high specific is high-throughput, and low cost detection is mentioned for the first time in early 1990s
After out, it is considered that there is huge development potentiality and broad application prospect in biomedical research.
Papery micro-fluidic chip (Paper-based Microfluidics) is a branch of micro-fluidic chip.2007
Year, the Whitesides group of Harvard University was put forward for the first time the micro-fluidic concept of papery, which introduces a kind of photoengraving pattern
Filter paper, the colorimetric analysis for urine pH and protein.Different from traditional silicon substrate or high polymer micro-flow control chips, papery miniflow
Control has the advantages such as bio-compatibility is good, detection background is low, pollution-free, paper easily obtains, it can be achieved that multiplexing, capillary force are driven
Dynamic sample liquids transport etc..The finger of detection (POCT) device by this effective bed proposed with the World Health Organization (WTO)
It is perfectly in harmony to lead principle.Hereafter, the micro-fluidic relevant academic research of papery has started volatile growth, and present papery is micro-fluidic
The application of chip has had been directed to the relevant every field of analytical chemistry, in food safety, general health monitoring, cancer early stage
Diagnosis, environmental monitoring etc. all achieve good effect.
Papery micro-fluidic chip substrate fabrication method is although varied, such as wax printing, inkjet printing, ultraviolet photolithographic, from
Daughter processing etc., but still be to prevent water to circumferential passages using wax as paper gap blocking material there are some problems, such as wax printing
Flowing be easily obtained although the price of wax is lower, production papery micro-fluidic chip need to beat using expensive wax spray
Print machine, and the barrier of wax formation resolution ratio in production is not high, and channel can obscure after heating, also be easy to cause organic solvent
Leakage.Inkjet printing is to be sprayed onto barrier on paper in the form of small liquid pearl by ink-jet printer, forms channel pattern.
The method can be selected material multiplicity but be limited to spray head property, and internal solution cannot be stored for a long time, and need repeatedly printing ability
Stablize pattern.Ultraviolet photolithographic is first to be solidified after one layer photoresist of spin coating or dipping blocking agent using laser in paper substrates
Reach the accuracy method of channel construction effect, but this needs high performance production instrument, complex steps, photoresist such as SU-8 light
The price of glue is also higher.One of plasma etching method is worn under exposure mask it is a known problem that being known from experience due to plasma (orifice) gas
Crossing causes following substrate to be often stretched, and leading to that treated, pattern is bigger than mask.To reduce reproducible degree.Due to
These reasons, the target user of papery micro-fluidic chip are the poor developing country and part poverty-stricken area of condition, these states
Family and area often do not have the condition for carrying out production papery micro-fluidic chip, cannot be well using this low cost, Gao Ling
Sensitivity, high-throughput analysis and detecting instrument, therefore, it is necessary to a kind of preparation methods of papery micro-fluidic chip to ask to solve this
Topic.
Summary of the invention
In view of the above existing problems in the prior art, the present invention provides a kind of preparation methods of papery micro-fluidic chip.
To solve prior art problem, the technical scheme adopted by the invention is as follows：
A kind of preparation method of papery micro-fluidic chip, includes the following steps：
Pattern template is prepared by processing the pattern with microchannel in template；
Liquid barrier is coated in the pattern, makes to form the barrier with the pattern on the pattern template
Seal；
Analysis paper is bonded with the barrier seal, penetrates into barrier positive and negative on the analysis paper same position
Two sides；
Heating or UV irradiation are carried out to the analysis paper that tow sides are impregnated with barrier, make barrier in the analysis paper
In solidified, formed papery micro-fluidic chip.
Further, the template is folding template, and the folding template includes at least two with the template for stirring side
Paper, and adjacent two Die plate papers are connected by stirring side, enable the analysis paper be clipped in two Die plate papers it
Between, and then the tow sides of the analysis paper is enable to be bonded simultaneously with the barrier seal.
Further, when pattern of the processing with microchannel is in template to be prepared into pattern template, by adjacent two
The Die plate paper makes adjacent two Die plate papers exist by reprocessing the pattern with microchannel after stirring edge contraction placement
It folds after placing, position of two patterns with microchannel respectively on two Die plate papers is identical.
Further, when pattern of the processing with microchannel is in template to be prepared into pattern template, in the template
On process the patterns with microchannel of at least two mirror images after, then by the adjacent template in two figures
It is folded at symmetry axis between case, makes the adjacent pattern with microchannel after folding respectively described in two
Position on Die plate paper is identical, wherein the symmetry axis between two patterns stirs side for the folding template.
Further, it is bonded with the barrier seal paper will be analyzed, so that barrier is penetrated into the analysis paper same
When tow sides on position, by the analysis folder between two folding templates with the barrier seal, with two
A sheet glass is pressed respectively against the outside of the folding template, and barrier is made adequately to penetrate into the tow sides of the analysis paper,
It is micro-fluidic to form the papery with barrier.
Further, by liquid barrier be coated in the pattern template on when, the coating method used for brushing method,
Spray coating method, one of dip coating and electrophoretic deposition.
Further, the barrier is organosilicon polymer, photoresist, epoxide resin polymer, thiophen polymer, urine
Alkanes polymer, the perfluor series material of hydrophobic oleophobic performance, long-chain fatty acid ester, the mixture containing organic gel and agent and pigment
One of.
Further, the barrier is that the PDMS in organosilicon polymer polymerize mixed liquor, the PDMS polymerization mixing
Liquid is PDMS solution and curing agent is 10 in mass ratio:1 mixed liquor.
Further, when the analysis paper for being impregnated with barrier to tow sides heats, by the analysis paper in 80-
At a temperature of 180 DEG C, 1-15min is heated, makes the barrier solidification penetrated into the analysis paper, forms the micro-fluidic core of papery
Piece.
Further, the template and analysis paper are NC Nitroncellulose paper, qualitative filter paper, chromatographic paper, blotting paper, nanometer
One of extrusion coating paper, softwood kraft, piezoelectricity paper, cellulose paper and printing paper.
The preparation method of papery micro-fluidic chip provided by the invention a kind of, by template processing graphic pattern prepare figure
Liquid barrier is impregnated with pattern template, then analysis paper is bonded with pattern template by case template, keeps barrier same from tow sides
When be immersed in analysis paper in, then by analysis paper heating make barrier solidification be connected to analysis paper in, form close and distant water-bound
Papery micro-fluidic chip；Preparation method provided by the invention can be prepared by simple template with microchannel pattern
Template, and make barrier from tow sides by using pattern template while immersing in analysis paper, not only accelerate the system of chip
Make speed, but also it is more uniform to enable to barrier to be distributed on analysis paper；Meanwhile preparation method provided by the invention
It is easy to operate, only by cutting pattern template, the requirement for meeting papery fluid channel can be formed without instrument and equipment, it is obtained micro-
Circulation road effectively prevents the seepage flow of liquid, for the micro-fluidic production of papery provide it is a kind of efficiently, low cost and practical, always
It, this method has easy to operate, rapid reaction, economical and practical and material relative to general papery micro-fluidic chip production method
The advantages that material easily obtains.
Detailed description of the invention
Fig. 1 is a kind of flow diagram of the preparation method of papery micro-fluidic chip of exemplary embodiment of the present.
Fig. 2 is the structural schematic diagram of the folding template of exemplary embodiment of the present.
In figure：1- folding template, 101- Die plate paper, 102- stir side.
Specific embodiment
In order to overcome the shortcomings in the prior art, the present invention provides a kind of preparation method of papery micro-fluidic chip.To make this
The purposes, technical schemes and advantages of invention are clearer, below in conjunction with the attached drawing in the preferred embodiment of the present invention, to this hair
Technical solution in bright embodiment is further described in more detail.Described embodiments are some of the embodiments of the present invention, and
The embodiment being not all of.The embodiments described below with reference to the accompanying drawings are exemplary, it is intended to it is used to explain the present invention, and
It is not considered as limiting the invention.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound
Every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.With reference to the accompanying drawing to this
The embodiment of invention is described in detail.
Embodiment 1
As shown in Figure 1, a kind of preparation method of papery micro-fluidic chip, includes the following steps：
S100, pattern template is prepared by processing the pattern with microchannel in template；
S200, liquid barrier is coated in pattern, makes to form the figuratum barrier seal of tool on pattern template；
S300, analysis paper is bonded with barrier seal, makes positive and negative two that barrier penetrates on analysis paper same position
Face；
S400, the analysis paper that tow sides are impregnated with barrier carry out heating or UV irradiation, make barrier in analysis paper
Solidified, forms papery micro-fluidic chip.
Preparation method provided by the invention can prepare the mould with microchannel pattern by simple folding template 1
Plate, and make barrier from tow sides by using pattern template while immersing in analysis paper, not only accelerate the production of chip
Speed, but also it is more uniform to enable to barrier to be distributed on analysis paper；Meanwhile preparation method behaviour provided by the invention
Make simply, only by cutting pattern template, the requirement for meeting papery fluid channel, miniflow obtained can be formed without instrument and equipment
Channel effectively prevents the seepage flow of liquid, provides for the micro-fluidic production of papery a kind of efficient, inexpensive and practical.
As a preferred embodiment, as shown in Fig. 2, template is folding template 1, folding template 1 includes at least two bands
There is the Die plate paper 101 for stirring side 102, and adjacent two Die plate papers 101 are connected by stirring side 102, analysis paper is enable to press from both sides
Between two Die plate papers, and then the tow sides for analyzing paper is enable to be bonded simultaneously with barrier seal.Folding template 1 is set
Meter not only facilitates processing of the pattern with microchannel on Die plate paper 101, while also facilitating analysis folder in two bands
Have between the folding template 1 of barrier seal, make analyze paper be clipped among pattern template in the form of sandwich, make barrier from
Tow sides are immersed in simultaneously in analysis paper, and barrier is made to penetrate into the tow sides on analysis paper same position；And it rolls over
The materials of folded template 1 are also relatively easy to, and a printing paper is such as carried out doubling and is processed, or is directly cut on a sheet of paper
Folding template 1 can be formed by folding after symmetric figure.
As a preferred embodiment, when pattern of the processing with microchannel is in template to be prepared into pattern template,
By adjacent two Die plate papers 101 by reprocessing the pattern with microchannel after stirring edge contraction placement, make adjacent two moulds
For paperboard 101 after folding placement, position of two patterns with microchannel respectively on two Die plate papers 101 is identical.
It as a preferred embodiment, is bonded paper will be analyzed with barrier seal, so that barrier is penetrated into analysis paper same
When tow sides on one position, folder will be analyzed between two 1 paper of folding template with barrier seal, with two glass
Glass piece is pressed respectively against the outside of folding template 1, and barrier is made adequately to penetrate into the tow sides of analysis paper, has resistance to be formed
It is micro-fluidic every the papery of agent.
As a preferred embodiment, in processing graphic pattern on to folding template 1, using hand cutting, graduating with cutter, laser and
Mechanical one of processing method processes folding template 1.
As a preferred embodiment, when liquid barrier is coated on pattern template, the coating method that uses for
Brushing method, spray coating method, one of dip coating and electrophoretic deposition.
As a preferred embodiment, barrier be organosilicon polymer using dodecyltrichlorosilane as representative, with
SU-8100 is that there is each series of representative photoresist, epoxide resin polymer, thiophen polymer, the urethane of hydrophobic nature to birds of the same feather flock together
Close perfluor series material, the macromolecule unitary by representative of wax of object, hydrophobic oleophobic performance by representative of Solvay-AD60X
The long-chain fatty acid ester of alcohol, using oily ink as one of mixture containing organic gel and agent and pigment of representative.
Further, barrier is that the PDMS in organosilicon polymer polymerize mixed liquor, and it is PDMS that PDMS, which polymerize mixed liquor,
Solution and curing agent are 10 in mass ratio:1 mixed liquor, wherein PDMS solution selects SYLGUARD-184A solution, curing agent
Select SYLGUARD-184B.It is mixed by PDMS solution and curing agent by this mass ratio, mixed liquor can be made to block mould
The pattern of microchannel is had on paperboard 101, and since the viscosity of mixed liquor makes mixed liquor be not easy to flow out in pattern.
As a preferred embodiment, when folder will be analyzed between two folding templates 1 with barrier seal,
It is pressed respectively against the outside of folding template 1 with two sheet glass, so that barrier is adequately penetrated into the tow sides of analysis paper, carrys out shape
It is micro-fluidic at the papery with barrier.
As a preferred embodiment, when being heated to the analysis paper that tow sides are impregnated with barrier, analysis paper is existed
At a temperature of 80 DEG C, 15min is heated, makes the barrier solidification penetrated into analysis paper, forms papery micro-fluidic chip.
As a preferred embodiment, template and analysis paper be template and analysis paper be NC Nitroncellulose paper, it is qualitative
One of filter paper, chromatographic paper, blotting paper, nano combined paper, softwood kraft, piezoelectricity paper, cellulose paper and printing paper.Its
In, nano combined paper is cellulose-carbon nano-tube extrusion coating paper, and cellulose-silver ink extrusion coating paper is nano combined paper, the fibre of representative
Tieing up plain paper is the ordered structure cellulose paper made from bottom to top.Wherein, the present invention uses the printing paper for being easier to obtain as mould
Plate material.
Embodiment 2
Referring to embodiment 1, a kind of preparation method of papery micro-fluidic chip provided in this embodiment is different to be in
In, in template processing with microchannel pattern to be prepared into pattern template when, at least two mirrors are processed in template
After the pattern with microchannel of picture, then adjacent template folded at the symmetry axis between two patterns,
Keep position of the adjacent pattern with microchannel after folding respectively on two Die plate papers identical, wherein two
Symmetry axis between pattern stirs side for folding template.
Embodiment 3
Referring to embodiment 1, a kind of preparation method of papery micro-fluidic chip provided in this embodiment is different to be in
In, when being heated to the analysis paper that tow sides are impregnated with barrier, by analyze paper 100 DEG C at a temperature of, heat 5min, make
The barrier solidification in analysis paper is penetrated into, papery micro-fluidic chip is formed.
Embodiment 4
Referring to embodiment 1, a kind of preparation method of papery micro-fluidic chip provided in this embodiment is different to be in
In, when being heated to the analysis paper that tow sides are impregnated with barrier, by analyze paper 180 DEG C at a temperature of, heat 1min, make
The barrier solidification in analysis paper is penetrated into, papery micro-fluidic chip is formed.
It should be noted last that the above specific embodiment is only used to illustrate the technical scheme of the present invention and not to limit it,
Although being described the invention in detail referring to example, those skilled in the art should understand that, it can be to the present invention
Technical solution be modified or replaced equivalently, without departing from the spirit and scope of the technical solution of the present invention, should all cover
In the scope of the claims of the present invention.
Claims (10)
1. a kind of preparation method of papery micro-fluidic chip, which is characterized in that include the following steps：
Pattern template is prepared by processing the pattern with microchannel in template；
Liquid barrier is coated in the pattern, makes to form the barrier print with the pattern on the pattern template
Chapter；
Analysis paper is bonded with the barrier seal, makes positive and negative two that barrier penetrates on the analysis paper same position
Face；
To the analysis paper that tow sides are impregnated with barrier carry out heating or UV irradiation, make barrier in the analysis paper into
Row solidification, forms papery micro-fluidic chip.
2. a kind of preparation method of papery micro-fluidic chip according to claim 1, which is characterized in that the template is folding
Folded template, the folding template includes at least two with the Die plate paper for stirring side, and adjacent two Die plate papers are by turning over
Dynamic side is connected, and the analysis paper is enable to be clipped between two Die plate papers, and then makes the tow sides of the analysis paper
It can be bonded simultaneously with the barrier seal.
3. a kind of preparation method of papery micro-fluidic chip according to claim 2, which is characterized in that processed in template
Pattern with microchannel is come when being prepared into pattern template, after adjacent two Die plate papers are placed by stirring edge contraction
Reprocessing has the pattern of microchannel, makes adjacent two Die plate papers after folding placement, and two have microchannel
Position of the pattern respectively on two Die plate papers is identical.
4. a kind of preparation method of papery micro-fluidic chip according to claim 2, which is characterized in that processed in template
Pattern with microchannel come when being prepared into pattern template, processed in the template at least two mirror images with miniflow
After the pattern in channel, then the adjacent template folded at the symmetry axis between two patterns, makes phase
Position of the adjacent pattern with microchannel after folding respectively on two Die plate papers is identical, wherein two
Symmetry axis between the pattern stirs side for the folding template.
5. a kind of preparation method of papery micro-fluidic chip according to claim 2, which is characterized in that will analyze paper with
The barrier seal fitting, when barrier being made to penetrate into the tow sides on the analysis paper same position, by the analysis
Folder is pressed respectively against the folding template with two sheet glass between two folding templates with the barrier seal
Outside makes barrier adequately penetrate into the tow sides of the analysis paper, micro-fluidic to form the papery with barrier.
6. a kind of preparation method of papery micro-fluidic chip according to claim 1, which is characterized in that obstructed by liquid
When agent is coated on the pattern template, the coating method used is brushing method, spray coating method, in dip coating and electrophoretic deposition
It is a kind of.
7. a kind of preparation method of papery micro-fluidic chip according to claim 1, which is characterized in that the barrier is
Organosilicon polymer, photoresist, epoxide resin polymer, thiophen polymer, urethane quasi polymer, hydrophobic oleophobic performance perfluor
One of series material, long-chain fatty acid ester, mixture containing organic gel and agent and pigment.
8. a kind of preparation method of papery micro-fluidic chip according to claim 7, which is characterized in that the barrier is
PDMS in organosilicon polymer polymerize mixed liquor, and the PDMS polymerization mixed liquor is PDMS solution and curing agent is in mass ratio
10:1 mixed liquor.
9. a kind of preparation method of papery micro-fluidic chip according to claim 1, which is characterized in that seeped to tow sides
When thering is the analysis paper of barrier to be heated, by it is described analysis paper 80-180 DEG C at a temperature of, heat 1-15min, make
The barrier solidification in the analysis paper is penetrated into, papery micro-fluidic chip is formed.
10. a kind of preparation method of papery micro-fluidic chip according to any one of claims 1 to 9, which is characterized in that institute
State template and analysis paper be NC Nitroncellulose paper, qualitative filter paper, chromatographic paper, blotting paper, nano combined paper, softwood kraft,
One of piezoelectricity paper, cellulose paper and printing paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810385689.2A CN108855257B (en) | 2018-04-26 | 2018-04-26 | Preparation method of paper microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810385689.2A CN108855257B (en) | 2018-04-26 | 2018-04-26 | Preparation method of paper microfluidic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108855257A true CN108855257A (en) | 2018-11-23 |
| CN108855257B CN108855257B (en) | 2020-06-02 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104437689A (en) * | 2014-11-21 | 2015-03-25 | 浙江大学 | Manufacturing method for microfluidic paper-based analytical device based on photosensitive seal printing |
| CN107233942A (en) * | 2017-05-09 | 2017-10-10 | 南京大学 | The paper chip and its preparation method and purposes of a kind of fast guiding and transport of substances |
| CN107462571A (en) * | 2016-06-02 | 2017-12-12 | 中国科学院理化技术研究所 | A kind of preparation method and application of the hydrophobic isolation pattern of paper substrate |
| CN107754961A (en) * | 2017-11-01 | 2018-03-06 | 南通大学 | The preparation method of papery microwell plate based on laser printing |
| KR20180023127A (en) * | 2016-08-24 | 2018-03-07 | 재단법인 대구경북첨단의료산업진흥재단 | Microfluidic bio-sensor manufacturing system of modular paper-based using xy-plotter and the method thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104437689A (en) * | 2014-11-21 | 2015-03-25 | 浙江大学 | Manufacturing method for microfluidic paper-based analytical device based on photosensitive seal printing |
| CN107462571A (en) * | 2016-06-02 | 2017-12-12 | 中国科学院理化技术研究所 | A kind of preparation method and application of the hydrophobic isolation pattern of paper substrate |
| KR20180023127A (en) * | 2016-08-24 | 2018-03-07 | 재단법인 대구경북첨단의료산업진흥재단 | Microfluidic bio-sensor manufacturing system of modular paper-based using xy-plotter and the method thereof |
| CN107233942A (en) * | 2017-05-09 | 2017-10-10 | 南京大学 | The paper chip and its preparation method and purposes of a kind of fast guiding and transport of substances |
| CN107754961A (en) * | 2017-11-01 | 2018-03-06 | 南通大学 | The preparation method of papery microwell plate based on laser printing |
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