CN107233942A - The paper chip and its preparation method and purposes of a kind of fast guiding and transport of substances - Google Patents
The paper chip and its preparation method and purposes of a kind of fast guiding and transport of substances Download PDFInfo
- Publication number
- CN107233942A CN107233942A CN201710320813.2A CN201710320813A CN107233942A CN 107233942 A CN107233942 A CN 107233942A CN 201710320813 A CN201710320813 A CN 201710320813A CN 107233942 A CN107233942 A CN 107233942A
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- Prior art keywords
- paper
- adhesive tape
- chip
- transparent plastic
- micro
- Prior art date
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- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000000126 substance Substances 0.000 title claims description 8
- 239000002390 adhesive tape Substances 0.000 claims abstract description 49
- 229920003023 plastic Polymers 0.000 claims abstract description 42
- 239000000758 substrate Substances 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000004033 plastic Substances 0.000 claims abstract description 8
- 239000000835 fiber Substances 0.000 claims abstract description 6
- 229920002521 macromolecule Polymers 0.000 claims abstract description 5
- 238000010030 laminating Methods 0.000 claims abstract description 3
- 238000001514 detection method Methods 0.000 claims description 27
- 239000012530 fluid Substances 0.000 claims description 14
- 238000000053 physical method Methods 0.000 claims description 5
- 238000007639 printing Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 4
- 238000007641 inkjet printing Methods 0.000 claims description 3
- 229910021645 metal ion Inorganic materials 0.000 claims description 3
- 241000555268 Dendroides Species 0.000 claims description 2
- 238000001259 photo etching Methods 0.000 claims description 2
- 238000001020 plasma etching Methods 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 238000001311 chemical methods and process Methods 0.000 claims 1
- 238000003698 laser cutting Methods 0.000 claims 1
- 238000004026 adhesive bonding Methods 0.000 abstract 1
- 238000003754 machining Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 43
- 239000007788 liquid Substances 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- 229920004890 Triton X-100 Polymers 0.000 description 8
- 239000013504 Triton X-100 Substances 0.000 description 8
- 238000004737 colorimetric analysis Methods 0.000 description 8
- 241000607142 Salmonella Species 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- -1 polyethylene terephthalate Polymers 0.000 description 4
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229910001430 chromium ion Inorganic materials 0.000 description 3
- 229910001431 copper ion Inorganic materials 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 229910001453 nickel ion Inorganic materials 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- XMPZTFVPEKAKFH-UHFFFAOYSA-P ceric ammonium nitrate Chemical compound [NH4+].[NH4+].[Ce+4].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O XMPZTFVPEKAKFH-UHFFFAOYSA-P 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- MVPPADPHJFYWMZ-UHFFFAOYSA-N chlorobenzene Chemical compound ClC1=CC=CC=C1 MVPPADPHJFYWMZ-UHFFFAOYSA-N 0.000 description 2
- 229910052804 chromium Inorganic materials 0.000 description 2
- 239000011651 chromium Substances 0.000 description 2
- 150000001875 compounds Chemical group 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002845 discoloration Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000021384 green leafy vegetables Nutrition 0.000 description 2
- 238000010147 laser engraving Methods 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 1
- UYYRDZGZGNYVBA-VPXCCNNISA-N (2s,3r,4s,5r,6r)-2-[2-chloro-4-[3-(3-chloro-4-hydroxyphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]phenoxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(Cl)C(O)=CC=2)C=C1Cl UYYRDZGZGNYVBA-VPXCCNNISA-N 0.000 description 1
- ISPYQTSUDJAMAB-UHFFFAOYSA-N 2-chlorophenol Chemical compound OC1=CC=CC=C1Cl ISPYQTSUDJAMAB-UHFFFAOYSA-N 0.000 description 1
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical class OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical class N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- UZFAXRHYDQFZTE-UHFFFAOYSA-N N-(3-hydroxyiminobutan-2-ylidene)hydroxylamine methanol Chemical compound CO.CC(C(=NO)C)=NO UZFAXRHYDQFZTE-UHFFFAOYSA-N 0.000 description 1
- VEQPNABPJHWNSG-UHFFFAOYSA-N Nickel(2+) Chemical compound [Ni+2] VEQPNABPJHWNSG-UHFFFAOYSA-N 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical group C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical class [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- FRTNIYVUDIHXPG-UHFFFAOYSA-N acetic acid;ethane-1,2-diamine Chemical class CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O.NCCN FRTNIYVUDIHXPG-UHFFFAOYSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- JOSWYUNQBRPBDN-UHFFFAOYSA-P ammonium dichromate Chemical compound [NH4+].[NH4+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O JOSWYUNQBRPBDN-UHFFFAOYSA-P 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 229910001447 ferric ion Inorganic materials 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- ISGXOWLMGOPVPB-UHFFFAOYSA-N n,n-dibenzylaniline Chemical compound C=1C=CC=CC=1CN(C=1C=CC=CC=1)CC1=CC=CC=C1 ISGXOWLMGOPVPB-UHFFFAOYSA-N 0.000 description 1
- KBJMLQFLOWQJNF-UHFFFAOYSA-N nickel(ii) nitrate Chemical compound [Ni+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O KBJMLQFLOWQJNF-UHFFFAOYSA-N 0.000 description 1
- XNGIFLGASWRNHJ-UHFFFAOYSA-N o-dicarboxybenzene Natural products OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000000059 patterning Methods 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920000131 polyvinylidene Polymers 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 235000011091 sodium acetates Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
A kind of paper substrate transparent plastic adhesive tape is combined micro-fluidic chip, and it is, using the paper with marking groove as base material, micro-fluidic chip to be combined with the paper substrate transparent plastic adhesive tape of transparent plastic adhesive tape gluing paper base material.This marking groove can constitute effective capillary channel by the laminating of plastic, transparent adhesive tape.While itself paper fiber capillarity is maintained, macromolecular substances are allowed directly to carry out fast transportation using capillary, this is improving paper chip conevying efficiency, is realizing that paper chip quantitatively detects, detects that macromolecular all has important practical significance, with higher practical value.Simultaneously because the machining manufacture at this interface is flexible, the facilities and equipment and raw material needed is cheap and easy to get, therefore compared to existing paper chip hollow capillary pipe manufacturing method, the inventive method has huge cost advantage, disclosure satisfy that production in enormous quantities, disposable requirement.The invention discloses its basic structure and preparation method.
Description
Technical field
The present invention relates to micro-fluidic chip, the compound structure in particular with paper and plastic, transparent adhesive tape has capillary channel
Micro-fluidic chip.
Background technology
Paper chip (Paper analytical devices (uPADs)) is from 2007 by Mario Martinez (Martinez) etc.
Since people introduces micro-fluidic field, pole has all been obtained in terms of chip is using material, chip manufacturing technology, chip operation mode
Big development.Traditional paper chip is made of paper, and paper is not only cheap and easy to get, is also easy to cut, is permeated, prints and dyes, so as to be applied to
Various patterning operations, including ink jet printing, block printing or even hand-written.These mature technologies are established for the development of paper chip
Basis is determined.In addition, paper chip is fundamentally distinguished by the loose structure of paper with traditional chip, this feature band
Two big advantages are carried out：First, due to the specific surface area that paper is larger, reagent is easier the absorption in paper so as to be fixed in paper,
So as to simplify the process of chip manufacturing；Second, due to the capillarity of paper fiber, liquid can voluntarily flow in paper, this
So that paper chip has broken away from the dependence to outside fluid injector, structure is more simple, will more applicable amateur field and general
The use of logical crowd.Therefore paper chip experienced fast development during the decade past, and it is best to be acknowledged as commercialization prospect
A kind of micro-fluidic chip.
Although however, quickly growing, paper chip still has some shortcomings compared with traditional microfluidic system.At these not
In foot, the liquid flow control problem of system is protruded the most.This control includes two aspects：Control and conveying model to liquid flowing speed
The control enclosed.In traditional individual layer paper chip, square it is directly proportional with flow distance the time required to the flowing of liquid, so with
The increase of distance, the time that liquid flows through whole pipe-line system also will substantially increase.This will cause obvious sample evaporation to be damaged
Consumption.So traditional paper chip is generally limited on a less construction unit (pipeline total length is generally less than 4cm), it is impossible to used
In the analysis of large-scale Multi-example.In traditional paper chip, transportable object is typically only limited to small molecule and ion, greatly
Absorption, chromatography occurs in molecule in paper chip, so that the problems such as occurring that serious distribution is uneven, be blocked in regional area.
In addition, traditional paper chip also faces manipulation multi-path fluid difficulty, is difficult to quantitative study, transmitting procedure absorption, sample weight
The problem of existing property difference.
Predicament for more than, has there is several preliminary solutions.One of which is sandwich construction.
Wax-patterned obtained paper chip pipeline is clipped in (S.Jahanshahi- between two layers of plastic thin films by Jahanshahi-Anbuhi
Anbuhi,P.Chavan,C.Sicard,V.Leung,S.M.Z.Hossain,R.Pelton,J.D.Brennan and
C.D.M.Filipe,Lab Chip,2012,12,5079–5085.ISSN：1473-0197)；Da Silva et al. are using rubbing
The polyethylene terephthalate thin slice for wiping raw electricity removes Packaging Paper base tube road (Everson T.S.G.da Silva, Murilo
Santhiago, Fabr í cio R.de Souza, Wendell K.T.Coltro and Lauro T.Kubota, Lab Chip,
2015,15,1651.ISSN：1473-0197).Two layers of Wax-patterned paper chip is directly stacked into one by Martinez et al.
Individual double-layer chip (A.W.Martinez, S.T.Phillips, G.M.Whitesides and E.Carrilho,
Anal.Chem.,2010,82,3–10.ISSN：0003-2700).This scheme can make to form gap between every layer of chip, hence it is evident that
Accelerate the flowing of liquid.Another resolving ideas is to use hollow pipeline, and Renault et al. initiate this scheme, and profit
Stacked with the Wax-patterned paper chip of paper fiber in two layers of removing pipeline, construct this hollow pipeline chip.Liquid can be
Because capillarity is with higher speed directed flow in this hollow pipeline, have higher use value (C.Renault,
X.Li,S.E.Fosdick and R.M.Crooks,Anal.Chem.,2013,85,7976–7979.ISSN：0003-2700 and
C.Renault,M.J.Anderson and R.M.Crooks,J.Am.Chem.Soc.,2014,136,4616–4623.ISSN
0002-7863).But this kind of cerul chip is not bent, insecure between stacking, easy leakage, and complexity can not be prepared
Structure.Based on above-mentioned cognition, the present invention starts to develop brand-new thinking, faster, is less adsorbed, with fixed with making flow rate of liquid
The paper chip of amount analysis macromolecular ability.
The content of the invention
It is an object of the invention to provide a kind of Novel paper capillary fast guiding knot for the quick transport of substances of paper chip
Structure and preparation method thereof, to meet the demand of the applications such as macromolecular quantitative analysis.
Technical scheme is as follows：
A kind of paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip, and it is in substrate paper with physical method or chemical method
Upper structure fluid channel, so that fluid to be analyzed flows according to the hydrophilic channel of setting, it is characterized in that：It is that master is micro- on substrate paper
Runner position forms transparent plastic adhesive tape of being fitted on wide 0.2-1mm and deep 0.1-1mm groove, groove, constitutes the paper of capillary
Chip.
Above-mentioned paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip, and described substrate paper can be the piece of fibre structure
Shape material, including all kinds of paper, plastics filter membrane or electrospun membrane.
Above-mentioned paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip, and described physical method can be wax spray printing, swash
The methods such as light cutting, printing, drawing, inkjet printing or seal impressing.
Above-mentioned paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip, and described chemical method can be photoetching, ultraviolet solid
The method such as change or plasma etching.
A kind of above-mentioned paper substrate-transparent plastic adhesive tape is combined the preparation method of micro-fluidic chip, and it comprises the following steps：
Step 1. paper base material with dendroid detection zone using physical method or chemical method manufacture；
Step 2. the center of paper to detection zone the delineation of main fluid channel position or imprint, if being allowed to form arterial highway equal
Even, obvious wide 0.2-1mm and deep 0.1-1mm groove；
Step 3. is fitted adhesive tape on the groove on the substrate paper that step 2 is made, and adhesive tape and groove is constituted hair
Tubule, cuts off unnecessary adhesive tape, produces paper substrate-transparent plastic adhesive tape and is combined micro-fluidic chip.
Above-mentioned paper substrate-transparent plastic adhesive tape is combined the preparation method of micro-fluidic chip, described in main fluid channel position impressing
Die marks can be used by going out groove.
Above-mentioned paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip in separation, analysis and/or identification organic molecule, nothing
Application in machine metal ion, macromolecule or large biological molecule.
Paper substrate-transparent plastic adhesive tape of the present invention is combined on micro-fluidic chip and collectively constitutes capillary with adhesive tape and substrate paper
Pipe, the main capillarity with capillary of transport of material in the chips in itself, without the capillarity by paper fiber, so that
Accelerate travelling speed and reduce the absorption of paper.
The specific effect of the present invention is as follows：
By the way that paper and plastic adhesive tape are combined, both having remained paper has the advantage of preferable wicking capacity, further through plastic cement
Band builds big capillary channel, accelerates the transmission speed of liquid, adds the effective propagation path of liquid, reduce fluid sample
Adsorption loss, based on above advantage, the chip is significant in being applied to the quantitative detection of life macromolecule.
In existing paper chip hollow capillary pipe manufacturing method, this method has huge cost advantage, disclosure satisfy that big
Batch production, disposable requirement.
Brief description of the drawings
Fig. 1 paper substrates-transparent plastic adhesive tape is combined micro-fluidic chip schematic diagram.The wherein basic structural representation of A. capillary wicks piece
Figure, the adhesive tape of substrate paper laminating.Wherein 4. transparent adhesive tape zone faces；3. substrate paper.Adhesive tape and substrate paper reeded one
Face is brought into close contact, and is collectively forming the small capillary on paper, is improved the efficiency of liquid transporting；B. paper substrate-transparent plastic adhesive tape
The basic structure schematic diagram of compound micro-fluidic chip, wherein the 1. main fluid channel capillary channels imprinted out；2. load detecting reagent
Circular paper substrate detection zone.Sample is detected to be carried on chip by the capillarity of main fluid channel (1) from bottom, and
Circle detection region is diffused into along the direction of the arrow in Fig., realizes the detection of sample.
Colour developing situation of the bovine serum albumin(BSA) (BSA) on chip in Fig. 2 embodiments 3 under various concentrations.Wherein A. is not
Develop the color situation during with BSA concentration；B. colour developing curve during different BSA concentration Jing Guo colorimetric analysis
Colour developing situation of the glutamic-oxalacetic transaminease (AST) on chip in Fig. 3 embodiments 3 under various concentrations.Wherein A. is different
Develop the color situation during AST concentration；B. colour developing curve during different AST concentration Jing Guo colorimetric analysis.Fig. 2 and Fig. 3 illustrate this
Feasibility of the chip in terms of large biological molecule is detected
Colour developing situation of the ferric ion solutions on chip in Fig. 4 embodiments 2 under various concentrations.Wherein A. difference iron from
Develop the color situation during sub- concentration；B. colour developing curve during different iron concentrations Jing Guo colorimetric analysis.
Colour developing situation of the nickel ion on chip in Fig. 5 embodiments 2 under various concentrations.Wherein A. difference nickel ions are dense
Develop the color situation when spending；B. colour developing curve during different nickel ion concentrations Jing Guo colorimetric analysis.
Colour developing situation of the chromium ion on chip in Fig. 6 embodiments 2 under various concentrations.Wherein A. difference chromium ions are dense
Develop the color situation when spending；B. colour developing curve during different chromium ion concentrations Jing Guo colorimetric analysis.
Colour developing situation of the copper ion on chip in Fig. 7 embodiments 2 under various concentrations.Wherein A. difference copper ions are dense
Develop the color situation when spending；B. colour developing curve during different copper ion concentrations Jing Guo colorimetric analysis.Fig. 4 to Fig. 7 illustrates this core
Feasibility of the piece in terms of conventional ion solution is detected
Colour developing situation of the Escherichia coli on chip in Fig. 8 embodiments 4 under various concentrations.Wherein A. difference large intestine bars
Develop the color situation during bacteria concentration；B. colour developing figure picture during different e. coli concentrations Jing Guo colorimetric analysis.
Colour developing situation of the salmonella on chip in Fig. 9 embodiments 4 under various concentrations.Wherein A. difference Salmonellas
Develop the color situation during bacteria concentration；B. colour developing figure picture during different salmonella concentration Jing Guo colorimetric analysis.Fig. 8 and Fig. 9 are illustrated
Feasibility of this chip in terms of microorganism is detected.
The colour developing pattern of 50mg/ml BSA and 500U/L AST mixed solutions in Figure 10 embodiments 3.Upside a line is circular
Detection zone is AST detection zones, and downside a line circle detection area is BSA detection zones.Detection zone all develops the color in figure,
Demonstrate detectability of the chip to large biological molecule mixed liquor.
The colour developing pattern of 100mg/L iron, nickel, chromium, copper mixed ion solutions in Figure 11 embodiments 2.Detection agent puts in order
Circulated three times for from left to right iron, nickel, chromium, copper.Detection zone all develops the color in figure, it was demonstrated that chip is to common metal ion
The detectability of mixed liquor.
Bacterium colony concentration is 10 in Figure 12 embodiments 44Cfu/ml Escherichia coli and 108Cfu/ml salmonella mixed liquor
Colour developing pattern.Detection agent puts in order and circulated six times for from left to right salmonella, Escherichia coli.Detection zone has been in figure
All colour developings, it was demonstrated that detectability of the chip to microorganism mixed liquor.
Embodiment：
The invention will be further described below in conjunction with the accompanying drawings.Accompanying drawing described herein is used for providing to enter the present invention
One step understands, constitutes the part of the application, and schematic description and description of the invention is used to explain the present invention, not structure
Into inappropriate limitation of the present invention.
Embodiment 1：Paper substrate-transparent plastic adhesive tape is combined the manufacture of micro-fluidic chip
Step 1. uses laser engraving filter paper to manufacture paper chip (Whatman middling speeds quantitative filter paper) as paper substrate, such as Fig. 1 institutes
Show；
Step 2. is carved out with laser engraving machine with width 0.5mm and depth 0.5mm groove polyethylene terephthalates
(PET) board mold；
Main fluid channel region in the middle of the paper chip obtained in step 1 is placed on the groove of mould by step 3., is imprinted out recessed
Groove is simultaneously pasted with plastic adhesive tape and seals whole groove；
Step 4. cuts off the unnecessary part of adhesive tape with scissors, that is, obtains paper substrate-transparent plastic adhesive tape and be combined micro-fluidic chip.
Embodiment 2：Paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip and analyzed simultaneously for different kinds of ions
Step 1. makes the paper chip in embodiment 1 with quadravalence detection zone；
Step 2. prepares the iron chloride that each concentration is respectively 0.1g/L, nickel nitrate, ammonium dichromate, copper sulphate hybrid standard
Liquid；
Step 3. prepares the buffer solution of pH 4.5 3M ammonium acetates；
Step 4. is molten with the hydroxylamine solution of the solution preparation 0.1g/ml in step 3 and 8mg/ml 1,10- Phens
Liquid；
Step 5. prepares 0.7mg/ml polyacrylic acid solution；
Step 6. prepares 0.5M NaF solution；
Step 7. prepares the buffer solution of pH 4.5 3M sodium acetates；
Step 8. prepares the dimethylglyoxime methanol solution that mass fraction is 1%；
Step 9. prepares 0.03M ammonia spirit；
Step 10. prepares 0.35mM ceric ammonium nitrate solutions；
Step 11. prepares the polyvinylidene diallyidimethylammonium chloride solution of mass fraction 5%；
Step 12. takes the hydrazine of 15mg 1,5- diphenyl phosphinylidyne two and 40mg phthalic acid acid anhydrides to be dissolved in 1ml acetone respectively；
Step 13. prepares the sodium-acetate buffers of 0.01M pH 4.5；
Step 14. takes 50mg bathocuproines and 10mg PEG-400 to be dissolved in 1ml chloroforms respectively；
1 μ l steps are successively added dropwise in the first order color development area that paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip in step 15.
Rapid 4 and step 5 in three kinds of solution preparing, be added dropwise at intervals of 3min；
1 μ l steps are successively added dropwise in the second level color development area that paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip in step 16.
The four kinds of solution prepared in rapid 6,7,8,9, are added dropwise at intervals of 3min；
1 μ l steps are successively added dropwise in the third level color development area that paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip in step 17.
The three kinds of solution prepared in rapid 10,11,12, are added dropwise at intervals of 3min；
1 μ l steps are successively added dropwise in the fourth stage color development area that paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip in step 18.
Rapid 13 and step 14 in two kinds of solution preparing, be added dropwise at intervals of 3min；
Paper substrate-transparent plastic adhesive tape is combined one section of immersion standard mixed liquor of micro-fluidic chip by step 19., treats solution in hair
Spy covers chip whole process under, is taken out after detection zone discoloration completely, with scanner (BROTHER, DCP-1618W) scanning simultaneously
Colourity is analyzed with ImageJ, Figure 11 is as a result seen.
Embodiment 3：Paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip and analyzed simultaneously for a variety of large biological molecule albumen
Step 1. makes the chip in embodiment 1 with seven rank detection zones；
Step 2. prepares 10mg/mL, 20mg/mL, 40mg/mL, 60mg/mL, 80mg/mL, 100mg/mL, 120mg/mL's
Bovine serum albumin(BSA) (BSA) phosphoric acid buffer (PBS) solution；
Step 3. prepares 10U/L, 20U/L, 40U/L, 60U/L, 80U/L, 100U/L, 200U/L, 300U/L, 500U/L's
Glutamic-oxalacetic transaminease (AST) phosphoric acid buffer (PBS) solution；
Step 4. prepares pH 1.8 250mM citrate buffer solutions 50ml (containing a drop Triton X-100
(Triton X-100))；
Step 5. prepares 95% ethanol solution of 6mM Tetrabromophenol Blues；
Step 6. prepares 10% aqueous trehalose solution；
Step 7.306mg cysteine sulfinic acids, 34mg α-ketoglutaric acids, 1.6mg ethylenediamine tetra-acetic acids (EDTA) are dissolved in 1ml
400mM Tris solution；
Step 8. dissolves successively in 25ml water：250mg polyvinyl alcohol, 100mg methyl greens, 50mg rhodamine Bs, 2.75mg
ZnCl2, one drop Triton X-100 (Triton X-100)；
Step 9. dissolves successively in 25ml water：250mg polyvinyl alcohol, 25mg methyl greens, 25mg rhodamine Bs, 2.75mg
ZnCl2, one drop Triton X-100 (Triton X-100)；
The μ l of solution 1.2 in step 4 are respectively added dropwise in chip-side circle detection area for step 10.；
Step 11. step 10 is completed after 3min, is respectively added dropwise in the region that solution in step 4 is added dropwise molten in 0.8 μ l steps 5
Liquid；
The solution that the opposite side in region is respectively added dropwise in 1 μ l steps 6 is added dropwise in step 10 in step 12.；
Step 13. step 12 is completed after 5min, and the solution in 1 μ l steps 7 is respectively added dropwise in the region that step 6 solution is added dropwise；
Step 14. step 13 is completed after 5min, and the solution in 1 μ l steps 8 is respectively added dropwise in the region that step 7 solution is added dropwise；
Step 15. step 14 is completed after 5min, and the solution in 1 μ l steps 9 is respectively added dropwise in the region that step 8 solution is added dropwise；
Step 16. step 15 is completed after 5min, by BSA the and AST solution of one immersion various concentrations of each chip, is treated
Solution covers chip whole process under capillary action, and detection zone is taken out after starting discoloration, with scanner (BROTHER, DCP-
1618W) scan and analyze colourity with ImageJ, as a result see Fig. 2.
In an experiment it can be found that with the increase of BSA and AST concentration, chip detection zone developer color starts
Now obvious change, colour developing result can use curve matching, so as to prove that this chip is transporting and loaded macromolecular complex
The ability that matter is reacted.
Embodiment 4：Paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip and detected simultaneously for multiple-microorganism
Step 1. makes the chip in embodiment 1 with three-level detection zone；
(chlorobenzene is phenol red, English full name Chlorophenol Red- β-D- by step 2. preparation 1mg/ml CPRG
Galactopyranoside) the aqueous solution；
Step 3. prepares 5mg/ml MC (the chloro- 3- indoles-caprylates of 5- bromo- 6, English full name 5-Bromo-6-chloro-
3-indolyl caprylate) aqueous solution；
Step 4. prepares Triton X-100 (Triton X-100) phosphoric acid buffer (PBS) of mass fraction 1%
Solution；
Step 5. gathers a small amount of Escherichia coli and salmonella with oese, and bacterium is dispersed in into 2ml steps 4 respectively matched somebody with somebody
In the solution of system.
100 μ l are respectively taken in the bacterium solution that step 6. is produced from step 5, are diluted step by step, flat board culture is used to determine bacterium solution
Bacterium colony concentration.
Remaining bacterium solution is put into ultrasonic cleaning instrument (Shu Mei boards KQ218 is cleaned by ultrasonic instrument) ice-water bath ultrasonic vibration by step 7.
15min, then according to volume ratio 1:5 ratio dilutes after three-level step by step to be mixed in equal volume.
Step 8. is respectively added dropwise under the μ l of solution 1.2 in step 2, room temperature condition in chip-side circle detection area and dried
5min；
Step 9. is respectively added dropwise under the μ l of solution 1 in step 3, room temperature condition in the opposite side circle detection area of chip and dried
The μ l of solution 1 that region is respectively added dropwise in step 3 again are added dropwise former after 5min；
Step 10. treats that solution exists by the Escherichia coli and salmonella solution of one immersion various concentrations of each chip
Covered under capillarity after chip whole process, chip is placed in sealed culture dish at 37 DEG C and is incubated 2h, with scanning after changing colour
Instrument (BROTHER, DCP-1618W) scans and analyzes colourity with ImageJ, as a result sees Figure 12.
In an experiment it can be found that with the increase of BSA and AST concentration, chip detection zone developer color starts
Now obvious change, so as to prove that this chip is transporting and loaded the ability that macromolecular substances are reacted.
Claims (7)
1. a kind of paper substrate-transparent plastic adhesive tape is combined micro-fluidic chip, it be with physical method or chemical method on substrate paper
Fluid channel is built, so that fluid to be analyzed flows according to the hydrophilic channel of setting, it is characterized in that：It is that master is micro- on substrate paper
Runner position forms the refill that laminating transparent plastic adhesive tape on wide 0.2-1mm and deep 0.1-1mm groove, groove constitutes capillary
Piece.
2. paper substrate according to claim 1-transparent plastic adhesive tape is combined micro-fluidic chip, it is characterized in that：Described base material
Paper is the flaky material of fibre structure, including all kinds of paper, plastics filter membrane or electrospun membrane.
3. paper substrate according to claim 1-transparent plastic adhesive tape is combined micro-fluidic chip, it is characterized in that：Described physics
Method is wax spray printing, laser cutting, printing, drawing, inkjet printing or seal method for stamping.
4. paper substrate according to claim 1-transparent plastic adhesive tape is combined micro-fluidic chip, it is characterized in that：Described chemistry
Method is photoetching, ultra-violet curing or plasma etching method.
5. paper substrate-transparent plastic adhesive tape described in a kind of claim 1 is combined the preparation method of micro-fluidic chip, it is characterized in that：It is wrapped
Include the following steps：
Step 1. paper base material with dendroid detection zone using physical method or chemical method manufacture；
Step 2. the center of paper to detection zone the delineation of main fluid channel position or imprint, if being allowed to form arterial highway uniform, bright
Aobvious wide 0.2-1mm and deep 0.1-1mm groove；
Step 3. is fitted adhesive tape on the groove on the substrate paper that step 2 is made, and adhesive tape and groove is constituted capillary,
Unnecessary adhesive tape is cut off, paper substrate-transparent plastic adhesive tape is produced and is combined micro-fluidic chip.
6. paper substrate according to claim 5-transparent plastic adhesive tape is combined the preparation method of micro-fluidic chip, it is characterized in that：It is described
Imprint out groove die marks in main fluid channel position.
7. paper substrate-transparent plastic adhesive tape described in claim 1 is combined micro-fluidic chip and is separating, analyzes and/or identifying organic
Application in molecule, inorganic metal ion, macromolecule or large biological molecule.
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| CN108855257A (en) * | 2018-04-26 | 2018-11-23 | 东北大学 | A kind of preparation method of papery micro-fluidic chip |
| CN111879768A (en) * | 2020-07-30 | 2020-11-03 | 上海化工研究院有限公司 | Multifunctional acid-base gradient rapid detection bottle with segmented color development function and application thereof |
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