CN108853601A - A kind of artificial skin and preparation method thereof - Google Patents
A kind of artificial skin and preparation method thereof Download PDFInfo
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- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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Abstract
The present invention provides a kind of artificial skin and preparation method thereof; which solve existing artificial skins often will appear after skin-grafting because of artificial skin bacteria resistance function lasting effectiveness difference or the unordered confusion of degradation; the technical issues of leading to skin-grafting failure; its double-layer structure being made of protection film layer, compact film, filled composite; protection film layer coating is connected to the upper surface of compact film; compact film is porous membrane structure; it is equipped with netted hole along three-dimensional in compact film, connection is covered with filled composite in hole；Protection film layer is wormwood extract, compact film is made of polycarboxylic acid anhydride and polyethylene glycol succinate mixed melting deposition modeling, filled composite is made of collagen, polylysine, glycine, Arabic gum, citric acid, the Chinese medical extract with antibiosis and antiviral functions, the present invention also provides the preparation methods of artificial skin, can be widely applied to medical ancillary technique field.
Description
Technical field
The present invention relates to medical ancillary technique field, in particular to a kind of artificial skin and preparation method thereof.
Background technique
Skin is the maximum organ of human body, it not only has the function of resisting external environment infection, and also undertaking prevents in vivo
The task that other substances such as moisture, electrolyte are lost.Under normal circumstances, if skin is by lighter damage can self be extensive
Multiple, when the skin of large area is by serious burn or damage, doctor must input immediately liquid and protect wound, if being only
The shallow-layer of skin is impaired, and new skin can regenerate.If patient receives serious burn, skin cannot be repaired on one's own account, lead to
The superficial skin at other positions of body must be often transplanted on wound, but this method can cause new scar.Moreover, if it is
The burn of large area, normal skin remain little, and will be unable to transplant.There is no the protection of skin, severe burn person just will appear
Serious dehydration and bacterium infection, when serious will crisis life, just need using artificial skin in this case.Leatheret
Skin is the principle and method of utilizing works He cell biology, the skin substitute products manually developed in vitro, for repairing, replacing
For the skin histology of defect, artificial skin is by artificial synthesized, the survival rate for the person that can greatly improve severe burn.
Whole world research at present, which answers used artificial skin mainly, following several classes：(1) high molecule plastic, synthesis polypeptide,
Film-form artificial skin made of the synthetic materials such as staple fibre；(2) regenerated protein and animal tissue's amnion, peritonaeum and fetal membrane etc.
Artificial skin made of biomaterial；(3) flake artificial skin made of complete poly- film.Many biomaterials such as hyalomitome
Acid, chitosan, chitin and the similar material with fibrous reticular structure be used to make synthesis film.The above all kinds of leatherets
Skin, in clinical application, currently used dermatoplastic method are sieve-like skin-grafting methods, artificial skin are cut before implantation many small
Mouthful, then in the case where some tension acts on, it is fixed in the skin surface by wound, is increasing the same of artificial skin area
When be also convenient for diffusate outflow, with the growth of artificial skin, these apertures can be with self-heal, and the plaque-like scar left can also
Gradually to subside.Existing artificial skin often will appear after the completion of skin-grafting because artificial skin bacteria resistance function lasting effectiveness is poor, lead
Cause trauma surface infestation to fester, or the unordered confusion of degradation, hinder to grow from body skin, eventually lead to skin-grafting failure, human skin compared with
Thick position carries out dermatoplasty there is a phenomenon where the above problems particularly evident.
Summary of the invention
The purpose of the present invention is to solve the deficiency of above-mentioned technology, a kind of artificial skin and preparation method thereof is provided, is made
Artificial skin has the effect of that medicine sustained and controlled release is put and successively degrades after skin-grafting, for new skin provide persistently it is good it is antibacterial with
Growing environment.
For this purpose, the present invention provides a kind of artificial skin, it is made of protection film layer, compact film, filled composite double
Layer structure, protection film layer coating are connected to the upper surface of compact film, and compact film is porous membrane structure, and compact film is interior along three
It ties up direction and is equipped with netted hole, connection is covered with filled composite in hole；Protection film layer is made of wormwood extract, compact film
It is made of polycarboxylic acid anhydride and polyethylene glycol succinate mixed melting deposition modeling, compact film is equipped with the first printable layer and second
The print wire of printable layer, the first printable layer and the second printable layer is that arch folds, and the first printable layer is equipped with the first arch
Recessed portion, the first arcuate recess are respectively facing left and right directions, and the second printable layer is equipped with the second arcuate recess, the second arcuate concave
Concave portion is respectively facing front-rear direction, and compact film is that the first printable layer is superimposed connection setting with the second printable layer alternating layers；It fills out
Fill compound by collagen, polylysine, glycine, Arabic gum, citric acid, with the Chinese medical extract of antibiosis and antiviral functions
It constitutes, the Chinese medical extract with antibiosis and antiviral functions includes wormwood extract；Protect film layer with a thickness of 50-200 μm；It causes
Close film layer with a thickness of 1-3mm；The aperture of hole is 100-800 μm, and the porosity of hole is 50-90%.
Preferably, the production raw material of film layer is protected to be made of the component of following parts by weight：The parts by weight of wormwood extract
Number is 1-5 parts；Appropriate deionized water；
The production raw material of compact film is made of the component of following parts by weight：20-50 parts of polycarboxylic acid anhydride；Poly-succinic second
20-50 parts of diol ester；20-50 parts of collagen；10-30 parts of polylysine；10-20 parts of glycine；5-10 parts of Arabic gum；Citric acid
5-10 parts；15-30 parts of Chinese medical extract with antibiosis and antiviral functions, with antibiosis and antiviral functions Chinese medical extract by
Wormwood extract and other Chinese medical extract compositions with antibiosis and antiviral functions, wherein wormwood extract 10-20 parts, other
5-10 parts of Chinese medical extract with antibiosis and antiviral functions；Appropriate deionized water；
Other Chinese medical extracts with antibiosis and antiviral functions are mint extract, Folium Isatidis extract, kuh-seng extraction
One of object, angelica extract, Angelica Dahurica extract, cornel extractive, Flos Magnoliae extract or more than one mixtures, with full
Demand of the sufficient human body for the anti-bacteria and anti-virus of different skin wound site.
A kind of preparation method of above-mentioned artificial skin comprising following steps：
(1), the production raw material of protection film layer according to parts by weight, is weighed respectively：1-5 parts of wormwood extract, deionized water
In right amount；
Weigh the production raw material of compact film：20-50 parts of polycarboxylic acid anhydride, 20-50 parts of polyethylene glycol succinate；Collagen
20-50 parts；10-30 parts of polylysine；10-20 parts of glycine；5-10 parts of Arabic gum；5-10 parts of citric acid；It is anti-with antibacterial
15-30 parts of the Chinese medical extract of virus function, wherein wormwood extract 10-20 parts, other are in antibiosis and antiviral functions
5-10 parts of medicament extract；Appropriate deionized water.
(2), the production raw material polycarboxylic acid anhydride of step (1) weighed compact film, polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.By polycarboxylic acid anhydride and poly-succinic ethylene glycol
Ester carry out melting be sufficiently mixed after, carry out raw material preparation for lower step.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 100-200 DEG C
Melting extrusion is carried out, after the first printable layer prints, the shaped platform of 3D printer declines the height of a printable layer, the
One printable layer upper surface forms the second printable layer, and repeating the above steps is superimposed the first printable layer and the second printable layer alternating layers
Connection is prepared into compact film until the printing of entire compact film terminates.3D printer is existing apparatus, using fusion sediment
Forming method successively stacks the first printable layer and the second printable layer alternating layers, until required compact film forms.Due to
The print wire of one printable layer and the second printable layer is that arch folds, and the arcuate recess of the first printable layer is towards right and left
To for the arcuate recess of the second printable layer towards front-rear direction, compact film is the first printable layer and the second printable layer alternating layer
Layer superposition connection setting is equipped in compact film along three-dimensional netted so that compact film after molding is porous membrane structure
Hole.
(4), by production raw material collagen, polylysine, glycine, the Arabic gum, lemon of step (1) weighed compact film
Lemon acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.Compact film inner pore is filled for lower step to be ready.
(5), compact film made from step (3) is added in mixture solution made from step (4), is vacuum-treated simultaneously
Progress ultraviolet light is taken out after impregnating 10-30min, then dry 0.5-1h at a temperature of 10-30 DEG C, is made in step (3)
Compact film hole in formed filled composite.It is vacuum-treated the pore interior gas of compact film made from step (3)
Body discharge, promotes mixture solution made from step (4) to be smoothly immersed in the hole of compact film, without bubble in hole,
The filled composite that the mixed solution of immersion is formed after ultraviolet light, drying process will not interrupt, internal each phase
It closes between raw material and realizes uniform adhesion between external and hole inner wall；Ultraviolet irradiation makes the glue in intrapore mixed solution
On the one hand primary raw cross-linking reaction enhances the cohesive force between filled composite and hole inner wall, another aspect filled composite
Internal each related raw material is not easily to fall off；Intrapore deionized water is evaporated in drying, keeps the cementation of Arabic gum more aobvious
It writes, the cohesive force inside enhancing filled composite between each related raw material, between filled composite and hole hole wall makes leatheret
After skin skin-grafting, the intrapore filled composite of compact film is not loose, does not fall off, reliable and stable, makes inside filled composite
It is uniformly distributed between each related raw material, has the effects that the comprehensive slow release of the raw material of anti-bacteria and anti-virus after facilitating skin-grafting, be
New skin, which provides, continues antibacterial environment and growing environment.
(6), step (5) are repeated repeatedly, until the hole in compact film fills up filled composite, is prepared into dense film
Layer.Surface through microscope observation artificial skin is to fill up without hole.
(7), the production raw material wormwood extract of the weighed protection film layer of step (1), deionized water are uniformly mixed, it is spraying
The outer surface of compact film coated in step (6) preparation, forms protective filmy layer layer, is finally prepared into artificial skin.Wormwood
Extract is uniformly mixed the outer surface for being sprayed on compact film with deionized water, forms protective filmy layer.
Preferably, the ultraviolet light in step (5) is to put the compact film for completing to impregnate on metal foil, ultraviolet lamp
Height 15-30cm, irradiation time 1-5h, and irradiation time gradually increases with the number that step (6) is impregnated.Promote compact film
Collagen cross-linking reaction in the mixed solution penetrated into hole is sufficiently completed.
Raw material Chinese medical extract of the present invention in terms of pharmacology it is as follows：
Wormwood extract：Wormwood system herbaceos perennial, there is higher medical value and long applicating history.It is modern
The pharmacological research of medicine shows that folium artemisiae argyi is a kind of antibacterial and antiviral drug of spectrum, he, which has a lot of bacteriums and virus, inhibits
And lethal effect.Its main chemical compositions has volatile oil, eudesmane, triterpenes, flavonoids, and there are also many microelements.Have
Anti-bacteria and anti-virus, antiallergy, calm immune, anti-platelet aggregation and other effects；To staphylococcus aureus, staphylococcus albus,
The bacterial cutaneous pathogenic bacteria such as Escherichia coli, bacillus subtilis have apparent inhibiting effect.And it is with unique acceleration
The effect of metabolism of deep skin cell inhibits platelet aggregation, accelerates blood circulation；Play it in artificial skin
Huge effect can greatly accelerate the speed of growth of human body defect skin.Using wormwood extract and other drugs into
Row compounding, can expand the scope of restraining fungi of this artificial skin, so that its biocidal property further enhances；
Mint extract：Herpesviral, vaccinia virus, Semliki forest virus and popular parotid gland virus can be inhibited equal
There is inhibiting effect, there is bacteriostasis to bacillus subtilis and proteus.
Folium Isatidis extract：Have to staphylococcus aureus, staphylococcus albus, alpha streptococcus, beta streptococcus
Apparent bacteriostasis；
Shrubby sophora extract：Pulse family Sophora machaka has pole to shigella dysenteriae, Escherichia coli, staphylococcus aureus
Strong bactericidal effect, sterilizing rate is 99% or more；There is different degrees of inhibition to make more than the ten kinds of dermatophytes such as a beautiful gem color hair Xian bacterium
With；Defined disinfection can be reached to air natural bacteria and surface disinfection effect to require；
Angelica Dahurica extract：With antibacterial action, to escherichia coli, shigella dysenteriae, typhoid bacillus, pseudomonas aeruginosa,
The bacteriums such as gram positive bacteria and Bacillus tuberculosis have different degrees of inhibiting effect；Have to dermatophytid infection good
Inhibiting effect；
Cornel extractive：To trichophyton, Trichophyton concentricum, trichophyton schoenleini, microsporum audouini, iron
Rust tinea bacterium, ulotrichy microsporum, epidermophyton inguinale, epidermophyton rubrum, Kaufman-Wolf Epidermophyton,
The dermatophytes such as star-shaped nocardia have different degrees of inhibiting effect；There is preferable effect for burn and scald；
Flos Magnoliae extract：To a variety of fungies such as trichophyton interdigitale, Candida albicans and staphylococcus aureus, B-mode hammer
Bacterium etc. has certain inhibiting effect.
The beneficial effects of the invention are as follows：
(1), the present invention has the effect of that good medicine sustained and controlled release is put and successively degrades after skin-grafting, provides for new skin
Continue good antibacterial and growing environment.The present invention does not hinder the growth of self skin and internal neoplastic skin, wound healing；
For cell in artificial skin inner surface, cell itself just will do it the growth and proliferation of itself, can be thin after being implanted into artificial skin
Born of the same parents provide the microenvironment for being more conducive to grow and be proliferated, but the presence of artificial skin occupies the space of cell growth and proliferation,
Therefore degradable artificial skin can gradually degrade out human skin cell growth and proliferation space, and provide nutriment,
Such as collagen, Arabic gum etc..Degradation includes hydrolysis and the processes such as cell degradation, therefore inner surface more meets the requirement of degradation more
Conducive to the generation of degradation, compact film on its chemical chain of polycarboxylic acid anhydride using other than the chemical bond with facile hydrolysis, will also having
Hydrophobic grouping appropriate is to make the hydrolysis rate of material surface greater than the rate for being diffused into material internal, therefore degradation process is
It gradually degrades outward from inner surface.Artificial skin after implantation, in the compact film inner surface being in contact with skin wound
Filled composite with sterilizing effect first plays a role, and used polycarboxylic acid anhydride has good superficial degradation
Performance, can guarantee artificial skin degradation be gradually degrade outward from inner surface so that with cell growing multiplication and
The degradation of artificial skin inner surface absorbs synchronous progress, and glycine, polylysine, citric acid, the Chinese medicine in filled composite mention
It takes object etc. to be gradually released, plays its effect, realize the fungistatic effect and wound healing promoting of artificial skin implantation all the period of time phase
Effect.Polyethylene glycol succinate is a kind of biodegradation type polyester, has good flexibility and thermal stability, mechanical
Better performances, processing performance is preferable, and polyethylene glycol succinate has preferable degradation property, mixes according to the proportion with polycarboxylic acid anhydride
It closes, adjusts the degradation rate of artificial skin from inside to outside, transplant demand to meet the human skin surface of a wound of different-thickness.Due to glue
Former presence makes artificial skin dense film, can prevent internal water, electrolyte, other substances from losing outward.
(2), there is weak irritation to cell while the present invention is with good biocidal property.The present invention is applied to clean wound
It when mouth or skin donor site, can be in close contact with skin wound, on the one hand guarantee that skin wound is sterile nontoxic, prevent bacterial invasion, have
There is stronger bacteria resistance function, skin wound can be protected not infected；On the other hand safe and convenient is used, pain can be effectively controlled
Bitterly, non-stimulated to skin wound or irritation is smaller.Polylysine can destroy the membrane structure of microorganism, cause cell
The interruption of substance, energy and information transmitting, moreover it is possible to which micro- life is killed in the synthesis that large biological molecule is influenced in conjunction with ribosomes intracellular
Object；And glycine has the function of the good formation for hindering bacteria cell wall；Citric acid can form complex compound with calcium ion,
So that blood clotting is obstructed, kills bacterial spore；Polylysine, glycine, the synergistic effect between citric acid greatly mention comprehensively
The inhibiting effect to microorganism is risen, makes artificial skin obtained that there is strong biocidal property.Resist in addition, the present invention is used with antibacterial
The Chinese medical extract of virus function, wherein wormwood extract is the drug with stronger antiallergy, anti-bacteria and anti-virus, to big portion
Partitivirus and bacterium play the role of inhibition and killing, mint extract, Folium Isatidis extract etc. other with anti-bacteria and anti-virus
The Chinese medical extract of effect is compounded with wormwood extract, significantly improves antibiosis and antiviral functions.Due to using medium-height grass
Medicament extract has lesser stimulation to the damaging cells of artificial skin implant site, so that obtaining good biocidal property
While can not injure normal cell as far as possible.Using polylysine, glycine, citric acid and with antibiosis and antiviral functions
Chinese medical extract collaboration is used in conjunction with, and is greatly improved its biocidal property and antiviral property, is also greatly improved wound site
Reparation rate.
(3), the present invention can guarantee that artificial skin does not damage when using sieve-like skin-grafting method, not fail.Using polycarboxylic acids
Acid anhydride and polyethylene glycol succinate pass through glue in the intrapore filled composite of compact film as matrix formation compact film
Former to pass through dry physical bonds by ultraviolet light irradiation chemical crosslinking and Arabic gum, being vacuum-treated in addition connects filled composite
It is continuous do not interrupt, uniform adhesion is on hole inner wall, the synergistic effect between three, each related raw material inside enhancing filled composite
Between and the cohesive force between filled composite and hole hole wall, further such that artificial skin inside generate greatly tie
With joint efforts；And compact film is that the first printable layer be superimposed to connect with the second printable layer alternating layers and is arranged, and the of the first printable layer
One arcuate recess is respectively facing left and right directions, and is respectively facing front-rear direction with the second arcuate recess, makes compact film shape
At tridimensional network, the first printable layer and the second printable layer are respectively that arch folds in addition, the first arcuate recess and second
The bottom print wire of arcuate recess is respectively positioned on the edge of artificial skin, and artificial skin itself is made to have stronger tear resistance
Energy.In short, artificial skin of the present invention has high resistance to fracture ability and is able to maintain certain flexibility and bending property, have
Some strength and elasticity, permeability is good, can effectively improve the tensile property of artificial skin, effectively prevents artificial skin from cutting
It is torn at mouthful, the success rate of operation is improved, so that this artificial skin is greatly applied.
(4), protecting the wormwood extract in film layer is the drug with stronger antiallergy, anti-bacteria and anti-virus, to very much
Virus and bacterium play the role of inhibition and killing；Outermost layer of the film layer as artificial skin is protected, external source is on the one hand prevented
Property bacterium and Virus entry body in, on the other hand prevent skin wound moisture content, electrolyte, other substances by compact film scatter and disappear
To external.Protection film layer is sprayed on the extexine of compact film, and after the completion of skin-grafting, artificial skin inner compact layer film layer and skin are created
Face closely connects, and wherein the upper end of the filled composite in compact film is connected with protection film layer, the lower end of filled composite
It is connected with skin wound, the drug for the compact film inner surface that artificial skin is connected after implanting with skin wound is first sent out
Raw effect, with the progress of degradation process, intermediate drug gradually discharges, when degradation proceeds to the middle and later periods, in compact film
Anti-bacteria and anti-virus raw material reduce, at this time artificial skin surface coating wormwood extract can also be through artificial skin dense film
The effect of outer surface diffusion of layer is penetrated into its internal neoplastic skin, is realized biocidal property and is promoted dermal cell growth metabolism,
Promote artificial skin in the lasting effectiveness of middle and later periods bacteria resistance function.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of main view of the invention；
Fig. 2 is the structural schematic diagram of left view shown in FIG. 1；
Fig. 3 is the structural schematic diagram of A-A cross-sectional view shown in FIG. 1；
Fig. 4 is the structural schematic diagram of B-B cross-sectional view shown in Fig. 2；
Fig. 5 is the structural schematic diagram of perspective view shown in FIG. 1；
Fig. 6 is the structural schematic diagram of compact film shown in FIG. 1；
Fig. 7 is the structural schematic diagram of top view shown in fig. 5；
Fig. 8 is the structural schematic diagram of the top view of the second printable layer shown in fig. 5；
Fig. 9 is the structural schematic diagram of the top view of the first printable layer shown in fig. 5；
Figure 10 is the structural schematic diagram of perspective view shown in fig. 5.
It is marked in figure：1. film layer is protected, 2. compact films, 3. filled composites, 4. holes, 5. first printable layers, 6. the
Two printable layers, 7. first arcuate recess, 8. second arcuate recess, the bottom print wire of 9. first arcuate recess, 10.
The bottom print wire of two arcuate recess.
Specific embodiment
The present invention will be further explained below with reference to the attached drawings and specific examples, to help the contents of the present invention are understood.
Method used in the present invention is conventional method unless otherwise required；Used raw material and device, such as without special rule
It is fixed, it is conventional commercial product.
Embodiment 1：
As shown in Fig. 1-Fig. 5, the present invention provides a kind of artificial skin, by protection film layer 1, compact film 2, filled composite
The double-layer structure that object 3 forms, protection film layer 1 coat the upper surface for being connected to compact film 2.
As shown in Fig. 1-Figure 10, protection film layer 1 is made of wormwood extract.Compact film 2 is porous membrane structure, dense film
Layer 2 is made of polycarboxylic acid anhydride and polyethylene glycol succinate mixed melting deposition modeling.Compact film 2 is equipped with the first printable layer 5
With the second printable layer 6, the print wire of the first printable layer 5 and the second printable layer 6 is that arch folds, the first printable layer 5
The arch of printing silk folds to form the first arcuate recess 7, and the first arcuate recess 7 is respectively facing left and right directions, the first arch
The bottom print wire 9 of recessed portion 7 is located at the left and right edges of artificial skin；The arch folding of the printing silk of second printable layer 6
Folded to form the second arcuate recess 8, the second arcuate recess 8 is respectively facing front-rear direction, and the bottom of the second arcuate recess 8 is beaten
Print line 10 is located at the front and rear edges of artificial skin.
First arcuate recess 7 is respectively facing left and right directions, and the second arcuate recess 8 is respectively facing front-rear direction, makes
The print wire of the print wire of first printable layer 5 and the second printable layer 6 is perpendicular, and compact film 2 is the first printable layer 5 and second
The superposition connection setting of 6 alternating layers of printable layer, the one side print wire of the first printable layer 5 and the print wire structure of the second printable layer 6
At the space network of protection film layer 1；On the other hand the first arcuate recess 7 of the first printable layer 5 and the second printable layer 6
Second arcuate recess 8 forms the netted hole 4 along three-dimensional.In surgical procedure, by the edge of artificial skin and human body skin
Skin cuts the suture of osculum edge, and the bottom print wire 9 of the first arcuate recess 7 is located at the left and right edges of artificial skin
Place, the bottom print wire 10 of the second arcuate recess 8 are located at the front and rear edges of artificial skin, make to have when suture certain
Tear resistance.
Connection is covered with filled composite 3 in hole 4, and filled composite 3 is by collagen, polylysine, glycine, Arab
Glue, citric acid, Chinese medical extract with antibiosis and antiviral functions are constituted, the Chinese medical extract with antibiosis and antiviral functions by
Wormwood extract and other Chinese medical extract compositions with antibiosis and antiviral functions, wherein other are with antibiosis and antiviral functions
Chinese medical extract be mint extract, Folium Isatidis extract, shrubby sophora extract, angelica extract, Angelica Dahurica extract, Fructus Corni
One of extract, Flos Magnoliae extract or more than one mixtures.
Protect film layer 1 with a thickness of 50-200 μm；Compact film 2 with a thickness of 1-3mm；4 aperture of hole is 100-800 μ
M, the porosity of hole 4 are 50-90%, meet human body different parts skin wound transplanting demand.
Embodiment 2：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：1 part of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：20 parts of polycarboxylic acid anhydride, 20 parts of polyethylene glycol succinate；20 parts of collagen；It is poly-
10 parts of lysine；10 parts of glycine；5 parts of Arabic gum；5 parts of citric acid；Chinese medical extract 15 with antibiosis and antiviral functions
Part, wherein 10 parts of wormwood extract, other 5 parts of Chinese medical extract with antibiosis and antiviral functions, are mint extract；Go from
Sub- appropriate amount of water.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 100 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 10min, then dry 0.5h, the densification made from step (3) at a temperature of 10 DEG C
Filled composite 3 is formed in the hole 4 of film layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 15cm, irradiation time 1h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Explanation：It can be extracted with Folium Isatidis extract, shrubby sophora extract, angelica extract, Angelica Dahurica extract, Fructus Corni
One of object, Flos Magnoliae extract replace mint extract.
Embodiment 3：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：5 parts of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：50 parts of polycarboxylic acid anhydride, 50 parts of polyethylene glycol succinate；50 parts of collagen；It is poly-
30 parts of lysine；20 parts of glycine；10 parts of Arabic gum；10 parts of citric acid；Chinese medical extract with antibiosis and antiviral functions
25 parts, wherein 20 parts of wormwood extract, other 5 parts of Chinese medical extract with antibiosis and antiviral functions, wherein mint extract 4
Part, 1 part of Folium Isatidis extract；Appropriate deionized water.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 200 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 30min, 1h, the dense film made from step (3) are then dried at a temperature of 30 DEG C
Filled composite 3 is formed in the hole 4 of layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 30cm, irradiation time 5h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Explanation：5 parts of other Chinese medical extracts with antibiosis and antiviral functions can extract for mint extract, folium isatidis
Object, shrubby sophora extract, angelica extract, Angelica Dahurica extract, cornel extractive, any two kinds in Flos Magnoliae extract.
Embodiment 4：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：3 parts of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：30 parts of polycarboxylic acid anhydride, 30 parts of polyethylene glycol succinate；30 parts of collagen；It is poly-
15 parts of lysine；15 parts of glycine；7 parts of Arabic gum；6 parts of citric acid；Chinese medical extract 21 with antibiosis and antiviral functions
Part, wherein 15 parts of wormwood extract, other 6 parts of Chinese medical extract with antibiosis and antiviral functions, wherein mint extract 4
Part, 1 part of Folium Isatidis extract, 1 part of shrubby sophora extract；Appropriate deionized water.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 160 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 15min, 1h, the dense film made from step (3) are then dried at a temperature of 15 DEG C
Filled composite 3 is formed in the hole 4 of layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 17cm, irradiation time 2h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Explanation：6 parts of other Chinese medical extracts with antibiosis and antiviral functions can extract for mint extract, folium isatidis
Object, shrubby sophora extract, angelica extract, Angelica Dahurica extract, cornel extractive, any three kinds in Flos Magnoliae extract.
Embodiment 5：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：3 parts of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：40 parts of polycarboxylic acid anhydride, 40 parts of polyethylene glycol succinate；30 parts of collagen；It is poly-
15 parts of lysine；15 parts of glycine；7 parts of Arabic gum；7 parts of citric acid；Chinese medical extract 22 with antibiosis and antiviral functions
Part, wherein 15 parts of wormwood extract, other 7 parts of Chinese medical extract with antibiosis and antiviral functions, wherein mint extract 3
Part, 2 parts of Folium Isatidis extract, 1 part of shrubby sophora extract, 1 part of angelica extract；Appropriate deionized water.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 160 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 15min, 1h, the dense film made from step (3) are then dried at a temperature of 15 DEG C
Filled composite 3 is formed in the hole 4 of layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 17cm, irradiation time 2h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Explanation：7 parts of other Chinese medical extracts with antibiosis and antiviral functions can extract for mint extract, folium isatidis
Object, shrubby sophora extract, angelica extract, Angelica Dahurica extract, cornel extractive, any four in Flos Magnoliae extract.
Embodiment 6：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：3 parts of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：30 parts of polycarboxylic acid anhydride, 30 parts of polyethylene glycol succinate；50 parts of collagen；It is poly-
20 parts of lysine；10 parts of glycine；7 parts of Arabic gum；9 parts of citric acid；Chinese medical extract 23 with antibiosis and antiviral functions
Part, wherein 15 parts of wormwood extract, other 8 parts of Chinese medical extract with antibiosis and antiviral functions, wherein mint extract 2
Part, 2 parts of Folium Isatidis extract, 2 parts of shrubby sophora extract, 1 part of angelica extract, 1 part of Angelica Dahurica extract；Appropriate deionized water.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 160 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 15min, 1h, the dense film made from step (3) are then dried at a temperature of 15 DEG C
Filled composite 3 is formed in the hole 4 of layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 17cm, irradiation time 2h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Explanation：8 parts of other Chinese medical extracts with antibiosis and antiviral functions can extract for mint extract, folium isatidis
Object, shrubby sophora extract, angelica extract, Angelica Dahurica extract, cornel extractive, any five kinds in Flos Magnoliae extract.
Embodiment 7：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：3 parts of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：30 parts of polycarboxylic acid anhydride, 30 parts of polyethylene glycol succinate；30 parts of collagen；It is poly-
30 parts of lysine；10 parts of glycine；7 parts of Arabic gum；8 parts of citric acid；Chinese medical extract 24 with antibiosis and antiviral functions
Part, wherein 15 parts of wormwood extract, other 9 parts of Chinese medical extract with antibiosis and antiviral functions, wherein mint extract 2,
Folium Isatidis extract 2, shrubby sophora extract 2, angelica extract 1, Angelica Dahurica extract 1,1 part of cornel extractive；Deionized water is suitable
Amount.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 160 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 15min, 1h, the dense film made from step (3) are then dried at a temperature of 15 DEG C
Filled composite 3 is formed in the hole 4 of layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 17cm, irradiation time 2h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Explanation：9 parts of other Chinese medical extracts with antibiosis and antiviral functions can extract for mint extract, folium isatidis
Object, shrubby sophora extract, angelica extract, Angelica Dahurica extract, cornel extractive, any six kinds in Flos Magnoliae extract.
Embodiment 8：
The method for preparing artificial skin described in embodiment 1 comprising following steps：
(1), the production raw material of protection film layer 1 according to parts by weight, is weighed respectively：5 parts of wormwood extract, deionized water are suitable
Amount；
Weigh the production raw material of compact film 2：30 parts of polycarboxylic acid anhydride, 30 parts of polyethylene glycol succinate；30 parts of collagen；It is poly-
25 parts of lysine；10 parts of glycine；7 parts of Arabic gum；7 parts of citric acid；Chinese medical extract 30 with antibiosis and antiviral functions
Part, wherein 20 parts of wormwood extract, other 10 parts of Chinese medical extract with antibiosis and antiviral functions, wherein mint extract 2
Part, 2 parts of Folium Isatidis extract, 2 parts of shrubby sophora extract, 1 part of angelica extract, 1 part of Angelica Dahurica extract, 1 part of cornel extractive,
1 part of Flos Magnoliae extract；Appropriate deionized water.
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film 2 of step (1), polyethylene glycol succinate are mixed equal
It is added in screw extruder after even and carries out mixing extrusion, be prepared into granular material.
(3), granular material prepared by step (2) is added in the extruder of 3D printer, is heated to 160 DEG C of progress
Melting extrusion, after the first printable layer 5 prints, shaped platform declines the height of a printable layer, the table on the first printable layer 5
Face forms the second printable layer 6, and repeating the above steps makes the first printable layer 5 and 6 alternating layers of the second printable layer be superimposed connection, until
The entire printing of compact film 2 terminates, and is prepared into compact film 2.
(4), by the production raw material collagen of the weighed compact film 2 of step (1), polylysine, glycine, Arabic gum,
Citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and it is molten to prepare resulting mixture
Liquid.
(5), compact film 2 made from step (3) is added in mixture solution made from step (4), is vacuum-treated
And progress ultraviolet light is taken out after impregnating 15min, 1h, the dense film made from step (3) are then dried at a temperature of 15 DEG C
Filled composite 3 is formed in the hole 4 of layer 2.Wherein ultraviolet light is that the compact film 2 for completing to impregnate is placed on metal foil
On, ultraviolet lamp height 17cm, irradiation time 2h.
(6), step (5) are repeated repeatedly, ultraviolet light irradiation time gradually increases with the number that step (6) is impregnated, directly
Filled composite 3 is filled up to the hole 4 in compact film 2, is prepared into compact film 2.
(7), the production raw material wormwood extract of the weighed protection film layer 1 of step (1), deionized water are uniformly mixed, spray
Mist is coated in the outer surface of the compact film 2 of step (6) preparation, forms protective filmy layer layer 1, is finally prepared into artificial skin.
Artificial skin prepared by 2~embodiment of above-described embodiment 8 is subjected to stretch-proof experiment respectively, antibacterial skin is examined
It surveys, degradability detection.
(1) the stretch-proof experiment of artificial skin
Using the measurement method of the skin properties of this field routine, the tensile strength of artificial skin is measured, as a result such as the following table 1
It is shown：
The tensile strength result of 1 artificial skin of table
As it can be seen from table 1 the artificial skin stretch intensity of the present invention is 8.1-10.5MPa, much higher than the drawing of human skin
Stretch intensity, make artificial skin have certain toughness, effectively prevent artificial skin and be torn from incision, guarantee skin-grafting at
Function.
(2) detection of artificial skin antibiotic property, degradability test experience
28 adult nude mices are chosen, after the skin wound of 1cm × 1cm is opened at every nude mice back, are divided into first group, second
Group, third group, the 4th group of totally four experimental groups, wherein first group 7, be successively implanted into respectively after number the embodiment of the present invention 2~
The artificial skin of embodiment 8；Second group 7, the leatheret of 2~embodiment of the embodiment of the present invention 8 is successively implanted into after number respectively
Skin；Third group 7, commercially available synthetic skin is successively implanted into after number respectively；It 4th group 7, is successively implanted into respectively after number commercially available
Artificial skin.First group, second group be used as embodiment, third group, the 4th group as a comparison case.(embodiment and comparative example is artificial
Skin is long 1cm, width 1cm)
After artificial skin is implanted into 8 days, the artificial skin that first group and third group nude mice are implanted into is removed respectively, observer
Make the degradation and nude mice skin surface of a wound growth and proliferation of cell situation of skin.First group of nude mice implantation artificial skin of the present invention from
It is interior to there is different degrees of degradation outside, it can be clearly seen that, the palliating degradation degree for the internal layer being in contact with skin wound is obviously fast
In the palliating degradation degree of outer layer, nude mice skin surface of a wound growth and proliferation of cell situation is preferable, illustrates growing multiplication and people with cell
The degradation for making inner skin surface absorbs synchronous progress, and artificial skin of the present invention meets successively degradation use demand, obtains expected good
Good effect.Palliating degradation degree is uniform from inside to outside for the commercially available synthetic skin of third group nude mice implantation, the growth of nude mice skin surface of a wound cell
Proliferative conditions are poor, illustrate that commercially available synthetic skin hinders growth and proliferation of cell, effect is poor.
The artificial skin of second group and the implantation of the 4th group of nude mice is when being degraded to 80%, the testing results such as antibiotic property such as following table
Shown in 2：
The antibiotic property detection of 2 artificial skin of table, degradability testing result
By above first group and third group, second group and the 4th group of comparison it is found that artificial skin of the present invention has after skin-grafting
The effect for having excellent medicine sustained and controlled release to put and successively degrade provides persistently good antibacterial and growing environment for new skin.
In the description of the present invention, it is to be understood that, term "left", "right", "upper", "lower", "top", "bottom", " preceding ",
The orientation or positional relationship of the instructions such as " rear ", "inner", "outside", " back ", " centre " is that orientation based on the figure or position are closed
System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have
Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
Only as described above, only specific embodiments of the present invention, when the model that cannot be limited the present invention with this and implement
It encloses, therefore the displacement of its equivalent assemblies, or according to equivalent changes and modifications made by the invention patent protection scope, should still belong to this hair
The scope that bright claims are covered.
Claims (4)
1. a kind of artificial skin, which is characterized in that its double-layer structure being made of protection film layer, compact film, filled composite,
The protection film layer coating is connected to the upper surface of the compact film, and the compact film is porous membrane structure, the densification
It is equipped with netted hole along three-dimensional in film layer, connection is covered with the filled composite in the hole；The protection film layer by
Wormwood extract is constituted, and the compact film is made of polycarboxylic acid anhydride and polyethylene glycol succinate mixed melting deposition modeling,
The compact film is equipped with the first printable layer and the second printable layer, the print wire of first printable layer and second printable layer
It is that arch folds, first printable layer is equipped with the first arcuate recess, and first arcuate recess is respectively facing
Left and right directions, second printable layer are equipped with the second arcuate recess, and second arcuate recess is respectively facing front-rear direction,
The compact film is that first printable layer is superimposed connection setting with the second printable layer alternating layers；The filled composite
Object is made of collagen, polylysine, glycine, Arabic gum, citric acid, the Chinese medical extract with antibiosis and antiviral functions,
The Chinese medical extract with antibiosis and antiviral functions includes wormwood extract；
It is described protection film layer with a thickness of 50-200 μm；The compact film with a thickness of 1-3mm；The hole aperture is 100-
800 μm, porosity 50-90%.
2. a kind of artificial skin according to claim 1, which is characterized in that the production raw material of the protection film layer is by following
The component of parts by weight is constituted：The parts by weight of wormwood extract are 1-5 parts；Appropriate deionized water；
The production raw material of the compact film is made of the component of following parts by weight：20-50 parts of polycarboxylic acid anhydride；Poly-succinic second
20-50 parts of diol ester；20-50 parts of collagen；10-30 parts of polylysine；10-20 parts of glycine；5-10 parts of Arabic gum；Citric acid
5-10 parts；15-30 parts of Chinese medical extract with antibiosis and antiviral functions, wherein wormwood extract 10-20 parts, other have anti-
5-10 parts of the Chinese medical extract of bacterium antivirus action；Appropriate deionized water；
Other described Chinese medical extracts with antibiosis and antiviral functions are mint extract, Folium Isatidis extract, kuh-seng extraction
One of object, angelica extract, Angelica Dahurica extract, cornel extractive, Flos Magnoliae extract or more than one mixtures.
3. a kind of preparation method of artificial skin according to claim 1 or 2, which is characterized in that it includes the following steps：
(1), the production raw material of the protection film layer according to parts by weight, is weighed respectively：1-5 parts of wormwood extract, deionized water
In right amount；
Weigh the production raw material of the compact film：20-50 parts of polycarboxylic acid anhydride, 20-50 parts of polyethylene glycol succinate；Collagen
20-50 parts；10-30 parts of polylysine；10-20 parts of glycine；5-10 parts of Arabic gum；5-10 parts of citric acid；It is anti-with antibacterial
15-30 parts of the Chinese medical extract of virus function, wherein wormwood extract 10-20 parts, other are in antibiosis and antiviral functions
5-10 parts of medicament extract；Appropriate deionized water；
(2), the production raw material polycarboxylic acid anhydride of the weighed compact film of the step (1), polyethylene glycol succinate is mixed
It is added in screw extruder after closing uniformly and carries out mixing extrusion, be prepared into granular material；
(3), the granular material prepared by the step (2) is added in the extruder of 3D printer, is heated to 100-
200 DEG C of progress melting extrusions, after the first printable layer printing, shaped platform declines the height of a printable layer, in institute
It states the first printable layer upper surface and forms second printable layer, repeating the above steps makes first printable layer and second dozen described
The superposition connection of layer alternating layers is printed, until the entire compact film printing terminates, is prepared into the compact film；
(4), by production raw material collagen, polylysine, the glycine, Arab of the weighed compact film of the step (1)
Glue, citric acid, the Chinese medical extract with antibiosis and antiviral functions, deionized water are mixed evenly, and mixing is prepared into
Object solution；
(5), the compact film made from the step (3) is added in mixture solution made from step (4), at vacuum
It manages and takes out progress ultraviolet light after impregnating 10-30min, then dry 0.5-1h at a temperature of 10-30 DEG C, in the step
(3) filled composite is formed in the hole of the compact film made from；
(6), repeating said steps (5) repeatedly, until the hole in the compact film fills up the filled composite, are prepared into
The compact film；
(7), production raw material wormwood extract, the deionized water of the weighed protection film layer of the step (1) are uniformly mixed,
The outer surface for the compact film that spray application is prepared in the step (6) forms one layer of protection film layer, final to prepare
At the artificial skin.
4. a kind of preparation method of artificial skin according to claim 3, which is characterized in that the institute in the step (5)
Stating ultraviolet light is to put the compact film for completing to impregnate on metal foil, ultraviolet lamp height 15-30cm, irradiation time
For 1-5h, and irradiation time gradually increases with the number that the step (6) is impregnated.
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