CN108853005A - Female genital tract containing superoxide dismutase repairs gel and preparation method thereof - Google Patents
Female genital tract containing superoxide dismutase repairs gel and preparation method thereof Download PDFInfo
- Publication number
- CN108853005A CN108853005A CN201810912633.8A CN201810912633A CN108853005A CN 108853005 A CN108853005 A CN 108853005A CN 201810912633 A CN201810912633 A CN 201810912633A CN 108853005 A CN108853005 A CN 108853005A
- Authority
- CN
- China
- Prior art keywords
- mass parts
- superoxide dismutase
- gel
- female genital
- genital tract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/155—Amidines (), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/44—Oxidoreductases (1)
- A61K38/446—Superoxide dismutase (1.15)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
- A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
- C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
- C12Y115/01001—Superoxide dismutase (1.15.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Gastroenterology & Hepatology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Gynecology & Obstetrics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Reproductive Health (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Urology & Nephrology (AREA)
- Inorganic Chemistry (AREA)
- Endocrinology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention provides a kind of female genital tract reparation gel containing superoxide dismutase, described to repair the raw material that gel includes following mass parts：Hepatocuprein, lysozyme, chlorhexidine acetate, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL, carbomer, oligopeptides -1.The present invention also provides preparation methods：Stock-emulsification-plastic.Main component of the invention is hepatocuprein, lysozyme, acetic acid chlorhexidine, oligopeptides -1 etc., and pure biology enzyme component, no hormone, antibiotic-free, ingredient is safe, without side-effects；Suitable for the daily routine care of women, antibacterial cleaning, taste of dispelling is antipruritic, repairs damage and mucosal barrier；Inhibit bacterium, the fungi of initiation gynecological disease, consolidates and improve the resistance of female reproductive system.Target user is extensive, including infant, the elderly, pregnant woman, puerpera.Application method is simple, is not required to rinse.
Description
Technical field
The present invention relates to a kind of, and the female genital tract containing superoxide dismutase repairs gel and preparation method thereof, belongs to woman
Section's health technology field.
Background technique
《Chinese science report》In the 6th edition skill of a doctor：The whole world has 46.6 ten thousand women to suffer from cervical carcinoma every year, and China has 13.2 ten thousand every year
Cervical carcinoma new cases account for the 28% of world's cervical carcinoma new cases sum, are 6 times of developed country, and annual is in rise
Gesture.
The gynecological disease of Chinese women ranks first in the whole world, and illness rate is up to 90% or more.In China, ten woman nine
A inflammation is absolutely not to paint an alarming picture of the situation just to scare the audience.Suffer from cervical carcinoma, because the women quantity that cervical carcinoma is passed away is even more that the whole world is the first！
Female sex organs do not only have vagina, including female internal genital organs and female external genital organs, vagina are women
Phallic a part.Female external genital organs refer to the exposed parts of reproductive organs, also known as vulva.Including mons pubes, labia majora, little Yin
Lip, clitoris, vestibular, greater vestibular gland, bulbus vestibuli, urethral orifice, introitus and hymen.Female internal genital organs include vagina, uterus,
Fallopian tubal, ovary etc..The privates of woman has the function of certain ego resistance, but is still highly susceptible to the invasion of bacterium,
It can defend and clean using gel help by extraneous.
Hormone or antibiotics ingredient are often added in many female genital tract gels in the market, benefit is can be fast
Speed tells on, but side effect is big, and Vaginal irritation effects are big.And some products are traditional Chinese medicine ingredients, drug effect is slow, the course for the treatment of is long.Have
It also needs to blend, rinse, use process trouble is awkward.
Summary of the invention
The present invention is in view of the deficienciess of the prior art, provide a kind of female genital tract reparation containing superoxide dismutase
Gel and preparation method thereof, to solve the problem of the existing big using trouble of female genital tract gel side effect.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of female genital tract containing superoxide dismutase
Gel is repaired, the reparation gel includes following raw materials according：
The superoxide dismutase of 300u/g-2000u/g,
The lysozyme of 1000u/g-5000u/g,
Chlorhexidine acetate,
Triethanolamine,
SIMMONDSIA CHINENSIS SEED OIL,
Carbomer,
Oligopeptides -1.
The present invention is further arranged to, described to repair the raw material that gel includes following mass parts：
The superoxide dismutase 5500-6500 mass parts of 800u/g,
The lysozyme 20500-25000 mass parts of 3000u/g,
Chlorhexidine acetate 3-30 mass parts,
Triethanolamine 3-300 mass parts,
SIMMONDSIA CHINENSIS SEED OIL 150-1500 mass parts,
Carbomer 3-900 mass parts,
- 1 3-1500 mass parts of oligopeptides.
The present invention is further arranged to, described to repair the raw material that gel includes following mass parts：
The superoxide dismutase 6000-6200 mass parts of 800u/g,
The lysozyme 22000-24000 mass parts of 3000u/g,
Chlorhexidine acetate 10-15 mass parts,
Triethanolamine 100-200 mass parts,
SIMMONDSIA CHINENSIS SEED OIL 500-700 mass parts,
Carbomer 100-200 mass parts,
- 1 8-30 mass parts of oligopeptides.
The present invention is further arranged to, described to repair the raw material that gel includes following mass parts：
6100 mass parts of superoxide dismutase of 800u/g,
23000 mass parts of lysozyme of 3000u/g,
12 mass parts of chlorhexidine acetate,
150 mass parts of triethanolamine,
600 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
150 mass parts of carbomer,
12 mass parts of oligopeptides -1.
The present invention is further arranged to, and the pH value for repairing gel is 4-8.
The present invention also provides above-mentioned, and the female genital tract containing superoxide dismutase repairs the preparation method of gel, including
Following steps：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It closes uniformly, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 60-90 DEG C from room temperature, heat preservation is cooled to
Room temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；Utilize high temperature or ultraviolet
Line gives modeling pipe sterilization, then filling, utilizes bottle placer in such a way that superoxide dismutase nursing gel is packed into propeller
It is dispensed, every gel content is 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
The present invention is further arranged to, and in step (2), is heated to 60-90 DEG C of time-consuming 1-1.5 hours from room temperature.
The present invention is further arranged to, and in step (2), keeps the temperature 1-2 hours.
The present invention is further arranged to, in step (2), cooling 0.5-1 hours to room temperature.
The effect that female genital tract of the present invention containing superoxide dismutase repairs the effective component in gel is as follows：
1, superoxide dismutase
Superoxide dismutase is superoxide dismutase important in organism, is distributed widely in various organisms,
Such as animal, plant, microorganism etc..Superoxide dismutase has special physiological activity, is that free radical is removed in organism
Primary substance.The effects of superoxide dismutase plays scavenging activated oxygen, anti-aging, after-sun recovery.
2, lysozyme
Lysozyme (lysozyme) is also known as muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-
Acetylmuramide glycanohydrlase), it is a kind of alkaline enzyme that can be hydrolyzed and stick polysaccharide in pathogenic bacteria.Main pass through is broken
The β between -acetylmuramic acid and n-acetylglucosamine-Isosorbide-5-Nitrae glycosidic bond in bad cell wall keeps cell wall insoluble glutinous more
Sugar decomposition causes cell wall rupture content to escape and make bacterolysis at soluble glycopeptide.Lysozyme can also with it is negatively charged
Virus protein bind directly, with DNA, RNA, apoprotein formed double salt, make virally inactivated.Therefore, the enzyme have antibacterial,
Anti-inflammatory, it is antiviral the effects of.
3, chlorhexidine acetate
Chlorhexidine acetate is also Robenidine Roripa, chlorhexidine acetate, Chlorhexidine, double benzyl chloride chlorhexidine, for sun
Ionic surface active agent, has a broad antifungal spectrum have killing effect to most Grain-positives and negative bacteria, also have to Pseudomonas aeruginosa
Effect.For remote holder of performing the operation, skin, planing surface and sterilization of instruments.
4, triethanolamine
I.e. three (2- ethoxy) amine, can regard the trihydroxy substituent of triethylamine as.It is similar to other aminated compounds,
Since, there are lone pair electrons, triethanolamine has alkalescent on nitrogen-atoms, salt can be generated with inorganic acid or organic acid reaction.
Be used as in cosmetics (including skin washing, eye glue, moisturizing, shampoo etc.) emulsifier, moisturizer, humidizer,
Thickener, PH poising agent.For being neutralized into soap with fatty acid in cosmetic formulations, amine salt is neutralized into sulfated fatty acid.
Triethanolamine and the colloid phase stability that higher fatty acids or higher aliphatic are formed are good, and stable product quality can hold outer addition
Divide specific gravity high.
5, SIMMONDSIA CHINENSIS SEED OIL
With high stability, be resistant to strong light, high temperature and keep structure constant, be the oil that can be hidden long.It has good
Permeability can permeate as long as there is gap；Also there is speciality resistant to high temperature, and the grease of molecules align and people very class
It seemingly, is stability is high, ductility is particularly good base oil, for lattice, SIMMONDSIA CHINENSIS SEED OIL is not in fact oily, the wax of liquid, institute
Can solidify when low temperature, scientist has found the unique liquid wax form of SIMMONDSIA CHINENSIS SEED OIL, has its unique molecular structure, unlike
Vegetable oil contained by SIMMONDSIA CHINENSIS SEED OIL ingredient, SIMMONDSIA CHINENSIS SEED OIL is the combination of a kind of alcohol polymer and fatty acid, therefore its structure
Abnormal stabilization, will not be by high temperature, and the grease property of this ingredient and skin surface is extremely close, so containing unlike
The skin care products of SIMMONDSIA CHINENSIS SEED OIL can form oil film in skin surface, and Jojoba provides two layers of effect to skin：Rapid osmotic absorption with
Softening skin cutin allows skin to become soft flexible.The mineral such as SIMMONDSIA CHINENSIS SEED OIL vitamin A rich in, B, E and calcium, magnesium
Matter, Jojoba Oil have very significant beauty functions to skin, can unimpeded pore, adjust the grease of oiliness or Combination skin
Secretion, and improve the skin of inflammation, eczema, chronic eczema, pimples etc..It has comparable curative effect and releives to allergic skin, for
Dry Hair and dryness wrinkle skin can make its gloss that rejuvenates, so being the base oil that various skin properties can use.
6, carbomer
Carbomer (carbomer) is a kind of as made of acrylic acid and the crosslinking of allyl saccharose or acrylic pentaerythrite
High molecular polymer is produced by Goodrich company of the U.S. earliest, has taken in the pharmacopeia of the states such as American and Britain, the medicine of China 2000 editions
Allusion quotation has also been recorded.Carbomer is a kind of excellent new type medicinal stuff, is widely used in researching and producing for cosmetics and drug
In.Carbomer be mainly used as in a medicament thickener, suspending agent, binder, gelling agent matrix and control sustained release preparation skeleton
Material etc..
Carbomer is white, loose, the micro- powder for having special smell, has stronger hygroscopicity, average moisture content 8%.Root
According to the different structure of polymer monomer.Carbomer is water-soluble, glycerol, ethyl alcohol, the characteristic with colloidal solution.Typical concentrations are
0.1%~3%, have the characteristics that water-soluble base by gel-type vehicle made from material of carbomer, releases the drug rapidly, without greasy
Property, be easy to apply exhibition, be nonirritant to skin and mucous membrane, can be mixed with aqueous solution and can absorptive tissue diffusate, be conducive to secrete
The exclusion of object.Compared with Vaseline basis, emulsifiable paste matrix, the advantages that there is easy to operate, quality stabilization, be not required to open firing,
It is particularly suitable for that volatile, thermal stability is poor, the preparation of clinical a small amount of urgently needed drugs.It is now widely used in gel for eye, skin
Skin gelling agent, mouth gels agent, nose bioadhesive polymer, vagina bioadhesive polymer etc..
7, oligopeptides -1 (EGF)
EGF is made of 53 amino acid, and the polypeptide including 3 disulfide bond.The molecular weight of EGF precursor is 130KD, volume
Also 300 times smaller than bacterium, the EGF gene of people is located at the long-armed of No. 4 chromosomes.EGF can promote the Proliferation, Differentiation of cell, from
And the process of senile cell and dead cell is replaced with neonatal cell.That Traditional makeup serves is outside modification, Bu Nengcong
Fundamentally solve skin problem, and it is physiological structure according to human skin in cosmetics that EGF, which is applied, in molecular level
On cell is repaired and is adjusted
EGF is unique cosmetic composition for obtaining Nobel and learning prize affirmative.The research achievement of EGF in 1999 is by world authority
The periodical U.S.《Science》Impurity is chosen as first of current year ten big sciences achievement." the beautiful factor " is described as by beauty circle.
Female genital tract of the invention, which repairs the superoxide dismutase in gel, has special physiological activity, is biology
The primary substance of free radical is removed in vivo.The effects of superoxide dismutase plays scavenging activated oxygen, anti-aging.For women
Genital tract, the mainly common bacterial infections such as inhibition staphylococcus aureus, Escherichia coli, Candida albicans.Suitable for women
Daily routine care, antibacterial cleaning, taste of dispelling is antipruritic, repairs damage and mucosal barrier；Inhibit to cause the bacterium, true of gynecological disease
The resistance of female reproductive system is consolidated and improved to bacterium.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
A kind of female genital tract reparation gel containing superoxide dismutase, the reparation gel includes following mass parts
Raw material：
6500 mass parts of superoxide dismutase of 800u/g,
20500 mass parts of lysozyme of 3000u/g,
3 mass parts of chlorhexidine acetate,
11 mass parts of triethanolamine,
290 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
3 mass parts of carbomer,
1200 mass parts of oligopeptides -1.
The present invention also provides above-mentioned, and the female genital tract containing superoxide dismutase repairs the preparation method of gel, including
Following steps：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It closes uniformly, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 90 DEG C from room temperature through 1 hour, heat preservation 1.2
Hour, cooling 1 hour to room temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；Utilize high temperature or ultraviolet
Line gives modeling pipe sterilization, then filling, utilizes bottle placer in such a way that superoxide dismutase nursing gel is packed into propeller
It is dispensed, every gel content is 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
Embodiment 2
A kind of female genital tract reparation gel containing superoxide dismutase, the reparation gel includes following mass parts
Raw material：
5500 mass parts of superoxide dismutase of 800u/g,
21000 mass parts of lysozyme of 3000u/g,
18 mass parts of chlorhexidine acetate,
3 mass parts of triethanolamine,
150 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
900 mass parts of carbomer,
1500 mass parts of oligopeptides -1.
The present invention also provides above-mentioned, and the female genital tract containing superoxide dismutase repairs the preparation method of gel, including
Following steps：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It closes uniformly, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 80 DEG C from room temperature through 1.2 hours, heat preservation
1.2 hours, cooling 0.5 hour to room temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；Utilize high temperature or ultraviolet
Line gives modeling pipe sterilization, then filling, utilizes bottle placer in such a way that superoxide dismutase nursing gel is packed into propeller
It is dispensed, every gel content is 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
Embodiment 3
A kind of female genital tract reparation gel containing superoxide dismutase, the reparation gel includes following mass parts
Raw material：
6200 mass parts of superoxide dismutase of 800u/g,
22500 mass parts of lysozyme of 3000u/g,
22 mass parts of chlorhexidine acetate,
17 mass parts of triethanolamine,
1500 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
300 mass parts of carbomer,
800 mass parts of oligopeptides -1.
The present invention also provides above-mentioned, and the female genital tract containing superoxide dismutase repairs the preparation method of gel, including
Following steps：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It closes uniformly, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 60 DEG C from room temperature through 1.5 hours, heat preservation 1
Hour, cooling 0.6 hour to room temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；Utilize high temperature or ultraviolet
Line gives modeling pipe sterilization, then filling, utilizes bottle placer in such a way that superoxide dismutase nursing gel is packed into propeller
It is dispensed, every gel content is 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
Embodiment 4
A kind of female genital tract reparation gel containing superoxide dismutase, the reparation gel includes following mass parts
Raw material：
6200 mass parts of superoxide dismutase of 800u/g,
24000 mass parts of lysozyme of 3000u/g,
30 mass parts of chlorhexidine acetate,
25 mass parts of triethanolamine,
700 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
600 mass parts of carbomer,
300 mass parts of oligopeptides -1.
The present invention also provides above-mentioned, and the female genital tract containing superoxide dismutase repairs the preparation method of gel, including
Following steps：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It closes uniformly, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 75 DEG C from room temperature through 1.2 hours, heat preservation 2
Hour, cooling 0.7 hour to room temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；Utilize high temperature or ultraviolet
Line gives modeling pipe sterilization, then filling, utilizes bottle placer in such a way that superoxide dismutase nursing gel is packed into propeller
It is dispensed, every gel content is 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
Embodiment 5
A kind of female genital tract reparation gel containing superoxide dismutase, the reparation gel includes following mass parts
Raw material：
6000 mass parts of superoxide dismutase of 800u/g,
25000 mass parts of lysozyme of 3000u/g,
10 mass parts of chlorhexidine acetate,
30 mass parts of triethanolamine,
920 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
810 mass parts of carbomer,
3 mass parts of oligopeptides -1.
The present invention also provides above-mentioned, and the female genital tract containing superoxide dismutase repairs the preparation method of gel, including
Following steps：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It closes uniformly, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 85 DEG C from room temperature through 1.5 hours, heat preservation
1.8 hours, cooling 0.8 hour to room temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；Utilize high temperature or ultraviolet
Line gives modeling pipe sterilization, then filling, utilizes bottle placer in such a way that superoxide dismutase nursing gel is packed into propeller
It is dispensed, every gel content is 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
Reparation gel of the invention can in gram ratio be configured to any quality, 30,000 be not limited in above-described embodiment
Gram, it can also be 3 kilograms, 20 kilograms, 40 kilograms or 80 kilograms etc., do not influence its performance.
Female genital tract containing superoxide dismutase of the invention repairs the comparison of gel and other washing lotions or gel
Advantage is as shown in the table：
The present invention has carried out multiple vaginal mucomembranous irritant test, and content is as follows：
One, experimental material：
1, tested material：
Sample property：Colorless and transparent jelly
2, experimental animal：Weight 2.4-3.0kg.Before execution standard GB14924.1-2001. test in animal house environment
Adapt to 2w.
3, testing conditions：20-23 DEG C of temperature range, RH range 50-70%.
Two, method
1, selection is healthy, Adult female new zealand rabbit 6 first, and whether there is or not secretion, hyperemia, water for pretest inspection rabbit introitus
Swollen and other degree of impairment, have inflammation or (and) damage the wounded abandons.Experimental animal is randomly divided into experimental group and control group, and every group 3
Rabbit.
2, the blunt nosed flexible conduit of length 8cm (No. 8 catheters) is connect with 2mL syringe.Syringe and catheter fill
Tested material is spare.Animal is faced upward fixation, perineum and introitus are exposed.Gently insertion is dynamic after conduit is moistened with test liquid
Object vagina (4-5cm), and syringe is slowly pushed into 2mL tested material, and gently withdrawal conduit, completes contamination.According to said method, every
Primary, the continuous 5d that contaminates is repeated for 24 hours.If there is spilling, gently wiped away with aseptic cotton.Control animals sterile saline is made same
Sample processing.
3, animal after contaminating for 24 hours, is put to death with aeroembolism method, cuts open the belly and takes out complete vagina, whether longitudinally slit, visually observing has
The performance such as hyperemia, oedema, for being referred to when pathologic sampling.Then vagina tissue sample is put into 10% formalin solution fixed
48h or more, the tissue film-making for choosing the both ends and central 3 positions of vagina carry out histopathological examination after HE dyeing.Group
Knit pathological examination result by《Disinfection technology standard》(2002 editions) 2.3.5.3 carries out vaginal mucosa irritation reaction scoring.
4, evaluation of result：Stimulate the reaction intensity is determined by vaginal mucosa irritation strength grading.
Three, result：
Discovery is visually observed, test group and control animals vaginal wall are essentially identical, there are no apparent hyperemia, oedema etc.
Performance.Histopathological examination the results are shown in Table 1.
The reaction scoring of 1. tested material Vaginal mucosa irritation test of table
Note：The stimulate the reaction integral addition/observation at 3 positions of average integral=3 animal is total (number of animals * 3)；Stimulation
Index=contamination group average integral-negative control group average integral.
Four, conclusion
The sample is 0 to rabbit vaginal mucosa irritation index, and vaginal mucosa stimulate the reaction intensity is absence of vagina irritation on mucous membrane.
The present invention has carried out antibacterial ring test, and content is as follows：
One, equipment
1. test strain：Escherichia coli 8099, staphylococcus aureus ATCC6538
2. culture medium：Nutrient agar
4. other：Vernier caliper etc..
Two, method
1. detecting foundation：The Ministry of Public Health《Technical norm for fire fighting》2002 editions 2.1.8.2 items.
2. testing conditions：37 DEG C are cultivated 18 hours, and test is repeated 3 times.
Three, result
It is tested through 3 repetitions, the results showed that the test specimen is to Escherichia coli, the inhibition zone diameter of staphylococcus aureus
Equal > 7.00mm, see the table below.
Note：The various recessive control equal < 7.00mm of antibacterial ring size.
Conclusion：Antibacterial ring size verification result shows that the female genital tract of the invention containing superoxide dismutase repairs gel
Fungistatic effect is all had to Escherichia coli, staphylococcus aureus and Candida albicans.
The present invention has carried out microbiological indicator test, and content is as follows：
One, method
1. detecting foundation：Disposable Sanitary Accessory sanitary standard GB15979-2002.
2. detecting temperature：19℃-21℃.
Two, result
Microbiological indicator is as a result, see the table below.
| Project | As a result |
| Total number of bacterial colonies (cfu/g) | < 4 |
| Escherichia coli | It is not detected |
| Pseudomonas aeruginosa | It is not detected |
| Staphylococcus aureus | It is not detected |
| Hemolytic streptococcus | It is not detected |
| Candida albicans | It is not detected |
| Fungus colony sum (cfu/g) | < 4 |
Three, conclusion：Female genital tract containing superoxide dismutase of the invention repairs the microbiological indicator result of gel
Meet《Disposable Sanitary Accessory sanitary standard GB15979-2002》Requirement.
Female genital tract for another kind containing complex enzyme repairs gel, and main component is antioxidase, lysozyme, vinegar
Sour chlorhexidine, triethanolamine, glycerol, carbomer, its advantages are：Pure biology enzyme component, no hormone, antibiotic-free, ingredient
Safety, it is without side-effects；Suitable for the daily routine care of women, antibacterial cleaning, taste of dispelling is antipruritic, repairs damage and mucosal barrier；Suppression
System causes bacterium, the fungi of gynecological disease, consolidates and improve the resistance of female reproductive system.Target user is extensive, including baby
Child, the elderly, pregnant woman, puerpera.Application method is simple, is not required to rinse.Women containing superoxide dismutase of the invention is raw
The advantages of growing reparation gel and the above-mentioned female genital tract reparation gel containing complex enzyme can see the table below：
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of female genital tract containing superoxide dismutase repairs gel, which is characterized in that under the reparation gel includes
Column raw material：
The superoxide dismutase of 300u/g-2000u/g,
The lysozyme of 1000u/g-5000u/g,
Chlorhexidine acetate,
Triethanolamine,
SIMMONDSIA CHINENSIS SEED OIL,
Carbomer,
Oligopeptides -1.
2. the female genital tract according to claim 1 containing superoxide dismutase repairs gel, which is characterized in that described
Repair the raw material that gel includes following mass parts：
The superoxide dismutase 5500-6500 mass parts of 800u/g,
The lysozyme 20500-25000 mass parts of 3000u/g,
Chlorhexidine acetate 3-30 mass parts,
Triethanolamine 3-300 mass parts,
SIMMONDSIA CHINENSIS SEED OIL 150-1500 mass parts,
Carbomer 3-900 mass parts,
- 1 3-1500 mass parts of oligopeptides.
3. the female genital tract according to claim 1 containing superoxide dismutase repairs gel, which is characterized in that described
Repair the raw material that gel includes following mass parts：
The superoxide dismutase 6000-6200 mass parts of 800u/g,
The lysozyme 22000-24000 mass parts of 3000u/g,
Chlorhexidine acetate 10-15 mass parts,
Triethanolamine 100-200 mass parts,
SIMMONDSIA CHINENSIS SEED OIL 500-700 mass parts,
Carbomer 100-200 mass parts,
- 1 8-30 mass parts of oligopeptides.
4. the female genital tract according to claim 1 containing superoxide dismutase repairs gel, which is characterized in that described
Repair the raw material that gel includes following mass parts：
6100 mass parts of superoxide dismutase of 800u/g,
23000 mass parts of lysozyme of 3000u/g,
12 mass parts of chlorhexidine acetate,
150 mass parts of triethanolamine,
600 mass parts of SIMMONDSIA CHINENSIS SEED OIL,
150 mass parts of carbomer,
12 mass parts of oligopeptides -1.
5. the female genital tract according to claim 1 containing superoxide dismutase repairs gel, which is characterized in that described
The pH value for repairing gel is 4-8.
6. -5 any female genital tracts containing superoxide dismutase repair the preparation side of gel according to claim 1
Method, which is characterized in that including step：
(1) it stocks up：Acetic acid chlorhexidine, carbomer, triethanolamine, SIMMONDSIA CHINENSIS SEED OIL and oligopeptides -1 are mixed according to the mass parts
It is even, superoxide dismutase and lysozyme soln, dissolution is added；
(2) it emulsifies：It is fitted into vacuum homogeneous mulser, stirs evenly, be heated to 60-90 DEG C from room temperature, heat preservation is cooled to often
Temperature；
(3) plastic：It stands overnight to get the superoxide dismutase nursing gel preparation；It is given using high temperature or ultraviolet light
Pipe sterilization is moulded, it is then filling, it is carried out in such a way that superoxide dismutase nursing gel is packed into propeller using bottle placer
Packing, every gel content are 3-3.5g；
(4) it detects：It randomly selects sample and carries out sample survey work and Experiment on Microbiology.
7. the female genital tract according to claim 6 containing superoxide dismutase repairs the preparation method of gel, special
Sign is, in step (2), is heated to 60-90 DEG C of time-consuming 1-1.5 hours from room temperature.
8. the female genital tract according to claim 6 containing superoxide dismutase repairs the preparation method of gel, special
Sign is, in step (2), keeps the temperature 1-2 hours.
9. the female genital tract according to claim 6 containing superoxide dismutase repairs the preparation method of gel, special
Sign is, in step (2), cooling 0.5-1 hours to room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810912633.8A CN108853005A (en) | 2018-08-12 | 2018-08-12 | Female genital tract containing superoxide dismutase repairs gel and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810912633.8A CN108853005A (en) | 2018-08-12 | 2018-08-12 | Female genital tract containing superoxide dismutase repairs gel and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108853005A true CN108853005A (en) | 2018-11-23 |
Family
ID=64317467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810912633.8A Pending CN108853005A (en) | 2018-08-12 | 2018-08-12 | Female genital tract containing superoxide dismutase repairs gel and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108853005A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110151981A (en) * | 2019-05-31 | 2019-08-23 | 杨平芝 | A kind of composition and preparation method thereof repaired vagina and treat vaginitis |
| CN112315900A (en) * | 2020-11-06 | 2021-02-05 | 南京道大药业有限公司 | Gel for nursing private parts of women |
| CN113577250A (en) * | 2021-08-11 | 2021-11-02 | 江苏格局生物医药科技有限公司 | Oxidoreductase-containing gel for repairing female genital tract and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1372974A (en) * | 2001-03-02 | 2002-10-09 | 凌沛学 | Exterior-applied gel contaiing lysozyme as primary component and its preparing process |
| CN104873432A (en) * | 2015-05-28 | 2015-09-02 | 南阳市汇博生物技术有限公司 | Hand-care disposable disinfection gel and preparation method thereof |
| CN106619186A (en) * | 2017-01-20 | 2017-05-10 | 吉林省拓华生物科技有限公司 | Preparation for protecting activity of superoxide dismutase (SOD) and preparation method of preparation |
| CN107536798A (en) * | 2017-08-29 | 2018-01-05 | 潘龙花 | A kind of female sex organ care compositions |
-
2018
- 2018-08-12 CN CN201810912633.8A patent/CN108853005A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1372974A (en) * | 2001-03-02 | 2002-10-09 | 凌沛学 | Exterior-applied gel contaiing lysozyme as primary component and its preparing process |
| CN104873432A (en) * | 2015-05-28 | 2015-09-02 | 南阳市汇博生物技术有限公司 | Hand-care disposable disinfection gel and preparation method thereof |
| CN106619186A (en) * | 2017-01-20 | 2017-05-10 | 吉林省拓华生物科技有限公司 | Preparation for protecting activity of superoxide dismutase (SOD) and preparation method of preparation |
| CN107536798A (en) * | 2017-08-29 | 2018-01-05 | 潘龙花 | A kind of female sex organ care compositions |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110151981A (en) * | 2019-05-31 | 2019-08-23 | 杨平芝 | A kind of composition and preparation method thereof repaired vagina and treat vaginitis |
| CN112315900A (en) * | 2020-11-06 | 2021-02-05 | 南京道大药业有限公司 | Gel for nursing private parts of women |
| CN113577250A (en) * | 2021-08-11 | 2021-11-02 | 江苏格局生物医药科技有限公司 | Oxidoreductase-containing gel for repairing female genital tract and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108853005A (en) | Female genital tract containing superoxide dismutase repairs gel and preparation method thereof | |
| RU2536264C2 (en) | Topical dermal composition containing salt and sugar as active ingredients for preventing and treating vaginosis, and using it | |
| CN105213298A (en) | A kind of oral care gels and preparation method thereof | |
| JP2022516983A (en) | Rhodococcus louver products and their pharmaceutical use | |
| CN106309471A (en) | Applications and preparation of low-molecular-weight biological active hyaluronic acid | |
| CN111481498A (en) | Antibacterial gel for regulating microecological balance of female vagina and preparation method thereof | |
| CN105663172A (en) | Ecological gynaecological externally-applied medicine composition and preparation method and application thereof | |
| CN106389139A (en) | Acne-removing, mark-eliminating and skin-protecting multifunctional preparation and preparation method thereof | |
| CN109395069A (en) | A kind of female genital tract reparation gel and preparation method thereof containing complex enzyme | |
| CN106491634B (en) | Hyaluronic Acid dimethyl-silicon alkanol compound and combinations thereof | |
| CN106109587A (en) | A kind of pharmaceutical composition repairing microecology in vaginas of women balance | |
| CN109316537A (en) | A kind of vaginal care antibacterial gel and preparation method and application | |
| CN109010529A (en) | A kind of Traditional Chinese medicine bacteriostatic gelling agent for vagina nursing | |
| CN110354224A (en) | A kind of Traditional Chinese medicine gel composition antibacterial for vaginal mucosa, antipruritic, taste of dispelling | |
| JP2022531109A (en) | Use of Rhodococcus louver products in the treatment of thermal injuries | |
| CN106581320A (en) | Preparation method of plant extract and preparation containing plant extract and used for women | |
| CN115089615B (en) | Nasal cavity microecological antibacterial restoration gel, and preparation method and application thereof | |
| CN111012902A (en) | Female antibacterial contraception maintenance gel and preparation method thereof | |
| Gupta et al. | Pluronic and Chitosan based in situ gel system for periodontal application | |
| CN114272261A (en) | A topical skin preparation containing povidone iodine for treating psoriasis | |
| CN106581780B (en) | Lubricant as well as preparation method and application thereof | |
| CN106177900B (en) | A kind of gel of anti-human papilloma virus (anti-HPV) and preparation method thereof | |
| CN105749260B (en) | Lysozyme hydrochloride vaginal tablet and preparation method and application thereof | |
| JPH02502639A (en) | Medicines for personal prevention of venereal diseases and treatment of urogenital trichomoniasis | |
| CN104013966B (en) | A kind of lotion and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 314001 Room 1301-2, 13/F, Block B, 705 Asia-Pacific Road, Nanhu District, Jiaxing City, Zhejiang Province Applicant after: Lishen Pharmaceutical Co.,Ltd. Address before: 314001 Room 1301-2, 13/F, Block B, 705 Asia-Pacific Road, Nanhu District, Jiaxing City, Zhejiang Province Applicant before: ZHEJIANG LISHEN PHARMACEUTICAL Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181123 |
|
| RJ01 | Rejection of invention patent application after publication |