CN108851199A - A kind of preparation method of enzymolysis glucomannan and the compound humectant of medicinal fungus fermentation material - Google Patents
A kind of preparation method of enzymolysis glucomannan and the compound humectant of medicinal fungus fermentation material Download PDFInfo
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- CN108851199A CN108851199A CN201810491981.2A CN201810491981A CN108851199A CN 108851199 A CN108851199 A CN 108851199A CN 201810491981 A CN201810491981 A CN 201810491981A CN 108851199 A CN108851199 A CN 108851199A
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- 239000003906 humectant Substances 0.000 title claims abstract description 43
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- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 title claims abstract description 39
- 238000000855 fermentation Methods 0.000 title claims abstract description 39
- 230000004151 fermentation Effects 0.000 title claims abstract description 39
- 150000001875 compounds Chemical class 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000463 material Substances 0.000 title claims abstract description 19
- 229920002752 Konjac Polymers 0.000 claims abstract description 115
- 235000013312 flour Nutrition 0.000 claims abstract description 29
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 13
- 235000010485 konjac Nutrition 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 27
- 229940088598 enzyme Drugs 0.000 claims description 25
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 20
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 20
- 150000004676 glycans Chemical class 0.000 claims description 17
- 244000061456 Solanum tuberosum Species 0.000 claims description 14
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 14
- 230000003519 ventilatory effect Effects 0.000 claims description 14
- 239000002054 inoculum Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 240000008397 Ganoderma lucidum Species 0.000 claims description 8
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 244000205754 Colocasia esculenta Species 0.000 claims description 5
- 235000006481 Colocasia esculenta Nutrition 0.000 claims description 5
- 229920001503 Glucan Polymers 0.000 claims description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 3
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 241001264174 Cordyceps militaris Species 0.000 claims description 2
- 241001313708 Dictyophora phalloidea Species 0.000 claims description 2
- 244000197580 Poria cocos Species 0.000 claims description 2
- 235000008599 Poria cocos Nutrition 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 238000013329 compounding Methods 0.000 claims description 2
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- 238000012549 training Methods 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 239000008107 starch Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 20
- 241000208125 Nicotiana Species 0.000 abstract description 14
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract description 14
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 abstract description 2
- 239000003546 flue gas Substances 0.000 abstract description 2
- 244000247812 Amorphophallus rivieri Species 0.000 abstract 1
- 235000001206 Amorphophallus rivieri Nutrition 0.000 abstract 1
- 239000000252 konjac Substances 0.000 abstract 1
- 235000019504 cigarettes Nutrition 0.000 description 11
- 241000222336 Ganoderma Species 0.000 description 6
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- 238000012545 processing Methods 0.000 description 5
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- 239000000758 substrate Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000012807 shake-flask culturing Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
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- 108010001682 Dextranase Proteins 0.000 description 1
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- 229930013930 alkaloid Natural products 0.000 description 1
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- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
Landscapes
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of preparation methods for digesting konjaku glucomannan and the compound humectant of medicinal fungus fermentation material.The present invention directly utilizes medicinal fungus Glucomannan crude enzyme liquid zymohydrolysis of konjaku flour, obtains a series of enzymolysis glucomannans with different molecular weight, and compound with medicinal fungus liquid deep layer fermenting object, the different novel konjac humectant of humectation effect is made.The present invention is a kind of konjaku deep process technology for combining Glucomannan and medicinal fungus effective component, have many advantages, such as high efficiency, strong operability, low cost preparation, the humectant of preparation is sprayed on pipe tobacco, it sucks safer, it is pollution-free, flue gas moisture content and jealous comfort level can be improved, its humectation effect is obviously improved compared to common humectant.
Description
Technical field
The present invention relates to Fermentation Engineering, enzyme engineering field and humectant for smoke fields, specifically, being related to by will be with evil spirit
Taro powder is the konjaku glycan that raw material obtains different molecular weight, and compounds medicinal edible fungus fermented object on this basis, prepares one
The biological preparation method of the compound humectant of kind.
Background technique
In the prior art, during production of cigarettes, in order to improve the physical property of tobacco, enhance the resist processing of tobacco
Property, making when reducing processing is broken, it usually needs the humectant with humectation effect is added.It is added in tobacco leaf (blade) and pipe tobacco
Humectant can improve the physical property of pipe tobacco, increase toughness, keep moisture, increase the softness of tobacco leaf (blade), pipe tobacco,
Making when reducing processing is broken, be lost, is conducive to product fragrance sends out thoroughly, has a very important role to cigarette quality.
Mainly use the polyhydroxy substances such as glycerol, propylene glycol, sorbierite for humectant in China's cigarette industry at present.But make
It is to maintain the moisture content of pipe tobacco in process with the main purpose of such humectant, improves the resist processing of pipe tobacco, and it is right
In the maintenance of finished cigarettes moisture content, sucking the improvement of comfort level, then effect is not satisfactory.In addition, the cigarette water of addition humectant
Dividing is influenced by ambient temperature and humidity, and the effect is not very satisfactory for humectation, and there are pipe tobacco moisture holding capacities, and poor, main flume moisture contains
The problems such as lower, dry sensation is obvious when sucking is measured, so, the performance improvement of humectant is still the technology that must currently solve
Problem.
Konjaku is the industrial crops of the relatively broad plantation of south China, because it uniquely can largely provide glucomannans
(KGM), then there is wide application value in fields such as medicine, food, chemical industry.Now studies have shown that the konjaku of different molecular weight
Glycan has different humectation effects because of the hydrophilic radical containing different number.Currently, being made with konjaku flour or konjaku powder
There are many technique that substrate produces the glycan of different molecular weight, it is main be by biological enzyme in conjunction with fermentation method, but must be to life
Object enzyme, which isolate and purify, can be only achieved significant effect, increase the complexity and cost of technique.With the technique by one
Biggest advantage is that substrate compares concentration, and yield is high, but also sends one's regards to topic, is exactly the raw material that KGM must be used purer, this
Sample increases cost, while and generating serious ring but also non-KGM substrate part is lost in raw material in hydrolytic process
Border pollution.Research shows that certain medicinal fungus can be very good degradation KGM, while can produce amylase, cellulase etc., it can be thorough
Hydrolyze effective substrate in konjaku flour, comprehensive degradation of realization konjaku active principle, while the different classes of molecular weight of its product
The glucan degree of polymerization compares concentration.
Medicinal fungus generally refer to those for medicine macro fungi.China has had the application of medicinal fungus more than 2500 years
History have no toxic side effect though their effects are different, therefore it is very popular.Wild medical fungi is usual and its rare
Preciousness, the main production and supply that medicinal fungus is carried out by artificial cultivation at present, but high administration fee and very long growth
Period is far from satisfying the demand of people.Submerged fermentation technology has become and obtains because of its cheap cost and of short duration fermentation period
Take an effective way of medicinal fungus.The medicines such as polysaccharide rich in, terpene, alkaloid, vitamin in medicinal fermented liquid
With bacterium active material, the type and content of effective component are above the fructification that cultivation obtains, significant preferable health-care effect.
Current konjaku product is mainly original konjaku glycan, and molecular weight is about 2000000 or so, has high viscosity
With extremely low dissolubility, application and development of the konjaku in food, chemical industry and biological health has been seriously affected.Develop different molecular
The enzymatic hydrolysis konjaku glucan series of amount, the diversified gesture of the maximum application and konjaku production of realizing konjaku product function is must
Row.Since biological polyoses effect of separate sources is different, as can obtaining the premise of not homology class konjaku glycan molecule amount realizing
Under, it is compounded with medical, edible granulose, will be expected to further expand the further technological processing way of konjaku.
Summary of the invention
The technical problem to be solved in the invention directly utilizes the crude enzyme liquid biological enzymolysis konjaku flour for being derived from medicinal fungus, orientation
Regulation reaction condition obtains a series of konjaku glucomannans with different average molecular weight, and according to product demand, by gained
The enzymatic hydrolysis konjaku glucomannan of specific average molecular weight is compounded with the medicinal fungus mycelium fermentation object containing konjaku effective component, is adjusted
Make a series of compound humectant of novel konjaku medicinal fungus with different humectation effects.
In order to solve the above technical problems, technical solution of the present invention is：A kind of enzymatic hydrolysis konjaku glucomannan and medicinal fungus
The preparation method of the compound humectant of fermentation material, the preparation method carry out according to the following steps：
(1) using concentration be 0.5~3.0% konjaku amidin as culture medium, and will generate extracellular Portugal's sweet dew gather
The medicinal fungus seed liquor of carbohydrase is added in konjaku culture medium according to the ratio of percent by volume 1%~20%, is placed in fermentor
In, it is 100~250r/min in revolving speed, fermentor ventilatory capacity is 0.5~2.0dm3/ min, under conditions of 24~32 DEG C of temperature
2~8d is cultivated, the culture solution that acquisition is filtered after culture is medicinal fungus Glucomannan crude enzyme liquid；
(2) then konjaku amidin gross mass 5% is added in the konjaku amidin that compound concentration is 0.5%~30%
The medicinal fungus Glucomannan crude enzyme liquid prepared in~20% the step of (1), and be 30 DEG C~70 DEG C in temperature, agitator speed
After digesting 10~360min under conditions of 20~100r/min, enzymolysis product is collected, sterilizing packing is to get enzymolysis glucomannan
The enzymatic hydrolysis konjak portuguese gansu polyose sugar juice that average molecular weight is 500~1000000；
It (3) is 0.3%~3% konjaku amidin as culture medium using mass volume ratio, in 20~37 DEG C of condition of temperature
Under, it takes medicinal fungus seed liquor to be added in konjaku culture medium according to the ratio of percentage by volume 5%~20%, is placed in fermentor
In, it is 0.8~2.0dm in fermentor ventilatory capacity3/ min, 5~10d of culture is obtained under conditions of revolving speed is 100~250r/min
Mycelium pellet suspension is mycelium fermentation object；
(4) the mycelium fermentation object in enzymatic hydrolysis konjak portuguese gansu polyose sugar juice and step (3) will be obtained in step (2) by quality
Than 0.1:1~1:0.1 ratio mixes compounding, obtains the compound humectant of konjaku glycan Yu medicinal fungus fermentation material.
The present invention preferably technical solution：The medicinal fungus seed of extracellular glucomannan enzyme can be generated in the step (1)
Liquid is to access medicinal fungus strain on potato slope culture medium to activate, and cultivates 3~6d, covers with inclined-plane to mycelia；Then with
Concentration is that 0.5~3% konjaku amidin is Shake flask medium, the medicinal fungus ramp blocks access konjaku flour shaking flask training after taking activation
Support the seed liquor that 3~4d of shaking flask culture is obtained under conditions of temperature is 24~32 DEG C, revolving speed is 100~250r/min in base.
The present invention preferably technical solution：The medicinal fungus seed liquor of inoculation in the step (3) is by medicinal fungus strain
It is activated on access potato slope culture medium, cultivates 3~6d, after mycelia covers with inclined-plane, taking ramp blocks access concentration is 0.2
In~3% konjaku amidin, 3~7d of shaking flask culture is obtained under conditions of temperature is 20~40 DEG C, 100~200r/min of revolving speed
The seed liquor obtained.
The present invention preferably technical solution：Konjaku flour in the step (1), step (2) and step (3) includes konjaku
Taro angle powder, konjaku powder, konjaku micropowder, purifying konjaku glucan in any one；And the konjaku flour in three steps is selected
Identical konjaku flour.
The present invention preferably technical solution：Medicinal fungus in the step (1) and step (3) includes ganoderma lucidum, mushroom, puts down
Mushroom, rainbow conk, needle mushroom, milky white rake bacterium, Poria cocos, Cordyceps militaris, Yunnan ferfas, any one in dictyophora phalloidea；And the medicine of two steps
Same or different medicinal fungus is selected with bacterium.
The present invention preferably technical solution：It is sweet containing β-Portugal in medicinal fungus Glucomannan crude enzyme liquid in the step (1)
Reveal dextranase, amylase, cellulase, which can realize that Multiple components are hydrolyzed in konjaku flour.
The present invention preferably technical solution：The fermented and cultured of medicinal fungus Glucomannan crude enzyme liquid is divided to two in the step (1)
Secondary progress, using concentration be 0.5~3.0% konjaku amidin as culture medium, the seed liquor that first obtains shaking flask culture
It is inoculated into the one grade fermemtation tank of 50L according to the inoculum concentration of percent by volume 1~10%, is 100~250r/ in speed of agitator
Min, fermentor ventilatory capacity are 0.5~2.0 dm3/ min after cultivating 1~4d under conditions of 24~32 DEG C of temperature, then presses volume hundred
Point than 5~20% inoculum concentration transfer 500L second order fermentation tank in, speed of agitator be 100~250r/min, fermentor
Ventilatory capacity is 0.5~2.0dm3/ min cultivates 1~4d under conditions of 24~32 DEG C of temperature.
The present invention preferably technical solution：The formula of the potato slope culture medium is：Potato：200g~300g/L, sugarcane
Sugar：15~25g/L.
Medicinal fungus strain in the present invention is the strain product directly bought.
Crude enzyme liquid used in the present invention carries out zymohydrolysis of konjaku flour and has the characteristics that：With the growth of zymohydrolysis of konjaku flour time,
The molecular weight of konjaku glucomannan sharply declines, enzymolysis speed with higher；With the growth of zymohydrolysis of konjaku flour time, more points
It dissipating sex index (PD) and weight average molecular weight drastically reduces, enzymatic hydrolysis konjaku glucomannan weight average molecular weight is 500~1000000, point
Dissipating sex index is 2~20, and product has preferable homogeneity.By controlling different enzymolysis times, obtaining has specified molecular weight
Uniform konjak portuguese gansu polyose sugar juice.
Compound humectant of the present invention, measure in the humectant of 100ml containing medicinal fungus polyoses content flat about 1.5~
15g, enzymatic hydrolysis konjaku glucomannan sugared content be 1.5~15g, enzymatic hydrolysis konjaku glucomannan average molecular weight be 500~
1000000。
It is an advantage of the invention that：
(1) the present invention utilizes green, health, efficient biofermentation and enzymatic hydrolysis using konjaku, medicinal fungus as raw material
Technology both produce the NEW TYPE OF COMPOSITE humectant with different humectation effects, while there is konjaku, medicinal fungus it is effective at
Point, humectation effect is more preferable, and can well using in cigarette；
(2) the humectant in the present invention is rich in a series of smaller, uniform, different molecular weight konjaku glycan, compared to other guarantors
Moisten agent, has humectation effect controllably and various matching with cigarette product, application value are more extensive.
(3) production operation of the invention is simple and easy, and low production cost, period are shorter, and production process is pollution-free, without useless
It abandons.
The present invention has many advantages, such as high efficiency, strong operability, low cost preparation, and the humectant of preparation is sprayed onto pipe tobacco
On, suck it is safer, it is pollution-free, flue gas moisture content and jealous comfort level can be improved, compared to common humectant its guarantor
Profit effect is obviously improved.
Specific embodiment
Below with reference to embodiment, the invention will be further described, the potato slope culture medium in following embodiment
Formula is：Potato：200g~300g/L, sucrose：15~25g/L.The konjaku culture medium is by konjaku flour and pure water
Konjaku amidin made of allotment, wherein the konjaku flour includes konjaku taro angle powder, konjaku powder, konjaku micropowder, purifying
Any one in konjaku glucan；Konjaku flour culture medium in each step selects identical konjaku flour.
Embodiment 1 provides a kind of low molecular weight enzymolysis glucomannan and the compound humectant of prepared from coriolus versicolor mycelium fermentation material
Preparation method, specific step is as follows：
(1) crude enzyme liquid obtains：Flat mushroom strain is accessed on potato slope culture medium and is activated, is cultivated 4 days, to mycelia
Inclined-plane is covered with, mushroom slant strains of making even are inoculated into 1% konjaku flour (mass volume ratio) shake flask culture, in temperature 28 DEG C, turn
Speed is the obtained seed liquor of culture 96h under 150r/min, then by seed liquor with 5% (percent by volume) inoculum concentration transfer into
In the first class seed pot of 50L (culture medium is as shaking flask), in revolving speed 150r/min, ventilatory capacity 1.5dm3/ min, temperature 28
After cultivating 48h under conditions of DEG C, then transfer (culture medium is as shaking flask) in 500L second order fermentation tank by 5% inoculum concentration,
Revolving speed 100r/min, ventilatory capacity 1.5dm3/ min, terminate after cultivating 48h under conditions of 28 DEG C of temperature, filtered after culture
The culture solution of acquisition is oyster mushroom Glucomannan crude enzyme liquid；The 1% konjaku flour shake flask culture is to add water to prepare with konjaku flour
Made of culture solution；
(2) low molecular weight konjaku glycan obtains：105kg konjaku flour is put into 5000L reactor, water is added to be configured to
The konjaku flour solution (do not have to sterilizing) of 3500L concentration 3%, after oyster mushroom Glucomannan crude enzyme liquid that will prepare in 350L step (1)
It is transferred in 5000L reactor, controlled at 60 DEG C, stirring degradation 300min, drop under conditions of agitator speed 100r/min
Solution finishes sterilizing, obtains the konjaku glycan solution that enzymolysis liquid, that is, weight average molecular weight is 5000 or so, and konjaku flour percent hydrolysis reaches
80.2%；
(3) prepared by rainbow conk seed liquor：It will directly buy and be preserved in the access potato slope culture of the rainbow conk strain in refrigerator
It is activated on base, cultivates 6d, after mycelia covers with inclined-plane, taking ramp blocks access to fill 100mL concentration is 0.5% konjaku culture
Liquid Culture 7d obtains seed liquor at revolving speed 150r/min, 26 DEG C in the 250mL shaking flask of base；
(4) prepared by prepared from coriolus versicolor mycelium fermentation material：In the konjaku culture medium that concentration is 1.5% (mass volume ratio), temperature
20 DEG C, inoculum concentration is 5% by percentage by volume, is 1.5 dm in fermentor ventilatory capacity3/ min, revolving speed are 150r/min culture 7
Its fermentation obtains prepared from coriolus versicolor mycelium fermentation material；
(5) the konjaku glycan solution and step (4) middle hair for being 5000 or so by the average molecular weight being prepared in step (2)
The prepared from coriolus versicolor mycelium fermentation material in mass ratio 1 that ferment obtains:2 ratio is deployed into a kind of Portugal that weight average molecular weight is 10000 or so
Sweet glycan and the compound humectant of prepared from coriolus versicolor mycelium fermentation material.
Embodiment 2：A kind of preparation of higher molecular weight enzymolysis glucomannan and the compound humectant of glossy ganoderma mycelium fermentation object,
Specific step is as follows for it：
(1) crude enzyme liquid obtains：Lucidum strain is accessed on potato slope culture medium and is activated, 4d is cultivated, it is long to mycelia
Full inclined-plane, takes ganoderma lucidum slant strains to be inoculated into 1% konjaku flour (mass volume ratio) shake flask culture, and 96h is cultivated at 30 DEG C and is obtained
To ganoderma lucidum seed liquor, ganoderma lucidum seed liquor is taken to transfer (culture medium in 50L first class seed pot with 10% (percent by volume) inoculum concentration
As shaking flask), it is 1.5dm in speed of agitator 150r/min, fermentor ventilatory capacity3/ min, it cultivates under conditions of 30 DEG C of temperature
It after 72h, then transfers (culture medium is as shaking flask) in 500L second order fermentation tank by 5% (mass volume ratio) inoculum concentration, is stirring
Mix revolving speed 100r/min, fermentor ventilatory capacity is 1.5dm3/ min, terminate after cultivating 72h under conditions of 30 DEG C of temperature, acquisition
Culture solution is ganoderma lucidum Glucomannan crude enzyme liquid；
(2) a kind of lower molecular weight konjaku glycan obtains：105kg konjaku flour is put into 5000L reactor, is configured to
The konjaku flour solution (do not have to sterilizing) that 3500L concentration is 3%, after by the thick enzyme of ganoderma lucidum Glucomannan obtained in 350L step (1)
Liquid is transferred in 5000L reactor, and controlled at 50 DEG C, degrade 10min, 30min, 60min respectively under 100r/min stirring,
It sterilizes after enzymatic hydrolysis every time, it is molten to obtain the konjaku glycan that weight average molecular weight is 100000,300000,700000 or so respectively
Liquid；
(3) prepared by ganoderma lucidum seed liquor：Cut-off connect buy and be preserved in the lucidum strain in refrigerator access potato slope it is enterprising
Row activation, is cultivated 4d days, and after mycelia covers with inclined-plane, taking ramp blocks access to fill 100mL concentration is 0.8% konjaku culture medium
250mL shaking flask in carry out Liquid Culture, revolving speed 150r/min, culture 5d obtains seed liquor at 30 DEG C；
(4) prepared by glossy ganoderma mycelium fermentation object：In the konjaku culture medium that concentration is 2% (mass volume ratio), temperature 30
DEG C, inoculum concentration is 5% by percentage by volume, and fermentor ventilatory capacity is 1.5dm3/ min, the condition that speed of agitator is 150r/min
Lower culture fermentation in 4 days obtains Ganoderma fermented product；
(5) three kinds of higher molecular weights being prepared in step (2) are digested into konjaku glycan, respectively according to 3:1 volume ratio
Glossy ganoderma mycelium fermentation object is added, is deployed into the enzyme that three kinds of weight average molecular weight are respectively 100000,300000,700000 or so
Solve konjaku glucomannan and the compound humectant of glossy ganoderma mycelium fermentation object.
Embodiment three：It is specific to select ten formulas for the humectation effect assessment comparison of the compound humectant of different molecular weight
Sample is diluted to by the compound humectant sample and a blank sample of ratio according to the method for perfuming cigarette with 70% ethyl alcohol
The solution that weight content is 30%, is uniformly sprayed in blank pipe tobacco by the 2% of pipe tobacco weight, cigarette is rolled into, in relative humidity
60 ± 2%, under conditions of 22 ± 1 DEG C of temperature, 48h or more is balanced, digests konjaku glycan and glossy ganoderma mycelium fermentation in sample
The ratio of object and the weight average molecular weight of compound humectant are specifically as shown in table 1,
1 sample situation of table illustrates table
The humectation effect evaluation method of this cigarette carries out the point system of humectant sense organ comfort level using in industry
Scoring, wherein score value is higher, and humectation effect is poorer, and specific appraisal result is as shown in table 2：
The compound humectant sensory comfort mean scores of table 2
As can be seen from Table 2, the compound humectant with different molecular weight compares blank control sample, every score value
With trend is decreased obviously, shows that humectation effect of the compound humectant in cigarette has and be obviously improved.
Claims (8)
1. a kind of preparation method of the compound humectant of enzymatic hydrolysis konjaku glucomannan and medicinal fungus fermentation material, the preparation method are pressed
Following steps carry out：
(1) using concentration be 0.5~3.0% konjaku amidin as culture medium, and extracellular glucomannan enzyme will be generated
Medicinal fungus seed liquor according to percent by volume 1%~20% ratio be added konjaku culture medium in, be placed in fermentor,
Speed of agitator is 100~250r/min, and fermentor ventilatory capacity is 0.5~2.0dm3/ min, culture 2 at a temperature of 24~32 DEG C~
8d, the culture solution that acquisition is filtered after culture obtain medicinal fungus Glucomannan crude enzyme liquid；
(2) compound concentration be 0.5%~30% konjaku amidin, then be added konjaku amidin gross mass 5%~
The medicinal fungus Glucomannan crude enzyme liquid prepared in 20% the step of (1), and be 30 DEG C~70 DEG C in temperature, speed of agitator is 20~
After digesting 10~360min under conditions of 100r/min, enzymolysis product is collected, sterilizing packing is to get enzymolysis glucomannan average mark
The enzymatic hydrolysis konjak portuguese gansu polyose sugar juice that son amount is 500~1000000；
(3) be 0.3%~3% konjaku amidin as culture medium using concentration, at 20~37 DEG C of temperature, take medicinal fungus seed liquor by
It is added in konjaku culture medium, is placed in fermentor according to the ratio of percent by volume 5%~20%, be in fermentor ventilatory capacity
0.8~2.0dm3/ min, speed of agitator are cultivated 5~10 days under conditions of being 100~250r/min and obtain mycelium pellet suspension i.e.
For mycelium fermentation object；
(4) the mycelium fermentation object in enzymatic hydrolysis konjak portuguese gansu polyose sugar juice and step (3) will be obtained in step (2) in mass ratio
0.1:1~1:0.1 ratio mixes compounding, obtains the compound humectant of konjaku glycan Yu medicinal fungus fermentation material.
2. the preparation of the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 1 and medicinal fungus fermentation material
Method, it is characterised in that：It is by medicinal fungus bacterium that the medicinal fungus seed liquor of extracellular glucomannan enzyme, which can be generated, in the step (1)
It is activated on kind access potato slope culture medium, cultivates 3~6d, cover with inclined-plane to mycelia；Then with concentration for 0.5~3%
Konjaku amidin is Shake flask medium, is in temperature in the medicinal fungus ramp blocks access konjaku flour Shake flask medium after taking activation
24~32 DEG C, revolving speed be 100~250r/min under conditions of the obtained seed liquor of 3~4d of shaking flask culture.
3. the preparation of the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 1 and medicinal fungus fermentation material
Method, it is characterised in that：The medicinal fungus seed liquor of inoculation in the step (3) is by the access potato slope training of medicinal fungus strain
It supports and is activated on base, cultivate 3~6d, after mycelia covers with inclined-plane, taking ramp blocks access concentration is 0.2~3% konjaku flour water
In solution, the seed liquor of 3~7d of shaking flask culture acquisition under conditions of temperature is 20~40 DEG C, 100~200r/min of revolving speed.
4. the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 1 or 2 and medicinal fungus fermentation material
Preparation method, it is characterised in that：Konjaku flour in the step (1), step (2) and step (3) includes konjaku taro angle powder, evil spirit
Taro fine powder, konjaku micropowder, purifying konjaku glucan in any one；And the konjaku flour in three steps selects identical konjaku
Powder.
5. the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 1 or 2 and medicinal fungus fermentation material
Preparation method, it is characterised in that：Medicinal fungus in the step (1) and step (3) includes ganoderma lucidum, mushroom, oyster mushroom, rainbow conk, gold
Needle mushroom, milky white rake bacterium, Poria cocos, Cordyceps militaris, Yunnan ferfas, any one in dictyophora phalloidea；And the medicinal fungus of two steps selects phase
Same or different medicinal fungus.
6. the preparation of the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 1 and medicinal fungus fermentation material
Method, it is characterised in that：Contain β-glucomannan enzyme, starch in medicinal fungus Glucomannan crude enzyme liquid in the step (1)
Enzyme, cellulase.
7. the preparation of the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 2 and medicinal fungus fermentation material
Method, it is characterised in that：The fermented and cultured of medicinal fungus Glucomannan crude enzyme liquid carries out in two times in the step (1), with dense
The konjaku amidin that degree is 0.5~3.0% is as culture medium, and the seed liquor for first obtaining shaking flask culture is according to percent by volume
1~10% inoculum concentration is inoculated into the one grade fermemtation tank of 50L, is 100~250r/min, fermentor ventilatory capacity in speed of agitator
For 0.5~2.0dm3/ min after cultivating 1~4d under conditions of 24~32 DEG C of temperature, then presses the inoculation of percent by volume 5~20%
Amount transfer 500L second order fermentation tank in, speed of agitator be 100~250r/min, fermentor ventilatory capacity be 0.5~
2.0dm3/ min cultivates 1~4d under conditions of 24~32 DEG C of temperature.
8. the compound humectant of a kind of enzymatic hydrolysis konjaku glucomannan according to claim 2 or 3 and medicinal fungus fermentation material
Preparation method, it is characterised in that the formula of the potato slope culture medium is：Potato：200g~300g/L, sucrose：15~25g/
L。
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| CN115554206A (en) * | 2022-10-21 | 2023-01-03 | 广州环亚化妆品科技股份有限公司 | Composite fermentation product for enhancing cell viability and preparation method and application thereof |
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