CN108851076A - A kind of preparation method and application of Bitter Melon P.E - Google Patents
A kind of preparation method and application of Bitter Melon P.E Download PDFInfo
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- CN108851076A CN108851076A CN201810575057.2A CN201810575057A CN108851076A CN 108851076 A CN108851076 A CN 108851076A CN 201810575057 A CN201810575057 A CN 201810575057A CN 108851076 A CN108851076 A CN 108851076A
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- bitter melon
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- 244000302512 Momordica charantia Species 0.000 claims abstract description 23
- 235000009811 Momordica charantia Nutrition 0.000 claims abstract description 23
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims abstract description 13
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- 230000004151 fermentation Effects 0.000 claims abstract description 6
- 230000003292 diminished effect Effects 0.000 claims abstract description 5
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 5
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- 206010012601 diabetes mellitus Diseases 0.000 claims description 11
- 239000000919 ceramic Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
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- 238000000926 separation method Methods 0.000 claims description 4
- 235000013402 health food Nutrition 0.000 claims description 2
- 238000003127 radioimmunoassay Methods 0.000 claims description 2
- 230000006837 decompression Effects 0.000 claims 1
- 238000005202 decontamination Methods 0.000 claims 1
- 230000003588 decontaminative effect Effects 0.000 claims 1
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- 241000219112 Cucumis Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
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- 210000000496 pancreas Anatomy 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010024229 Leprosy Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
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- 206010052428 Wound Diseases 0.000 description 1
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- 239000003963 antioxidant agent Substances 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- CTYRPMDGLDAWRQ-UHFFFAOYSA-N phenyl hydrogen sulfate Chemical compound OS(=O)(=O)OC1=CC=CC=C1 CTYRPMDGLDAWRQ-UHFFFAOYSA-N 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
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- 230000008439 repair process Effects 0.000 description 1
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- 238000000638 solvent extraction Methods 0.000 description 1
- 150000005856 steroid saponins Chemical class 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 150000008130 triterpenoid saponins Chemical class 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of preparation methods of Bitter Melon P.E, the preparation method of Bitter Melon P.E obtained by being extracted using the full fruit of balsam pear as raw material, extraction process proposes extraction by water at low temperature, extracting solution is filtered under diminished pressure to obtain filtrate, concentrating filtrate to relative density is 1.10~1.15, obtain Bitter Melon P.E medicinal extract, ferment is added in Bitter Melon P.E medicinal extract after removal of impurities and carries out cold fermentation, nanoscale embedding is carried out using specific embedding medium, with slow releasing function, it smashes it through 80 meshes and obtains Bitter Melon P.E, extracting method of the present invention is simple and quick, extraction cost is low, and the Bitter Melon P.E of preparation, the content 5.10uIU/ml of every gram of Bitter Melon P.E finished product insulin is surveyed out by radioimmunology.
Description
Technical field
The present invention relates to food to lead field, and in particular to a kind of preparation method and application of Bitter Melon P.E.
Background technique
Balsam pear alias brocade lichee, toad, leprosy grape, balsam pear, red sheep are Curcurbitaceae Momordica herbaceous plants, bitter
Melon bitter taste is cold, have it is clearing heat and detoxicating, nourish strong, hypoglycemic, anti-mutation, be antitumor and the function such as improve the immunity of the human body
Effect, is one of favorite melon vegetables of people.
Research shows that containing the drop such as a large amount of flavones, saponins (steroid saponin and triterpenoid saponin), para-insulin in balsam pear
Sugared active constituent can be developed into the accesary foods for the treatment of diabetes.Currently, the whole world in 2000 is reported by the World Health Organization (WHO)
Diabetic in range has been up to 1.6 hundred million.Existing 50,000,000 people of diabetic in China or so, the number of diabetic
It is only second to India and occupies second place of the world.About the treatment of diabetes, international diabetes conference proposes the " modern for the treatment of diabetes
Composite treatment " --- diet conservancy treatment, exercise therapy, drug therapy, self-monitoring, knowledge education and psychotherapy.This is just
Determine important function of the hypoglycemic health-care product in treating diabetes.Diabetes are a kind of chronic, lifelong diseases, control blood
Sugar level needs lifelong medication, this just needs antihypelipidemic foodstuff to have the characteristics that be easy to receive and have no toxic side effect, be suitble to be used for a long time,
Blood glucose level, ancillary drug treatment can be effectively adjusted simultaneously.Since present diabetic is numerous, so effective hypoglycemic food
Product have extensive demand.Blood-sugar lowering type health-care food market is huge, and has the huge market space and consumption demand, and benefit very may be used
It sees.Nearly 100,000,000,000 yuan/year of its market capacity according to a preliminary estimate.
Currently have some using balsam pear extract as the patent and document of antioxidant and hypoglycemic health-care product.Usually
Balsam pear is first gone capsule deseeding, is stripped after chopping using hot water, alcohol or other organic solvents, then resin is utilized to extract
Further progress isolates and purifies, and finally obtains required product.These technical process only extract in balsam pear part effectively at
Point, and a large amount of effective component, such as plant insuline vegetable protein, it is lost, while existing permitted during the extraction process
The problem of more effective components inactivate.These process requirements consume a large amount of organic solvent, spend very big cost.Production process meeting
The pollutants such as a large amount of organic solvent are discharged, environment is polluted.Therefore be badly in need of developing effective preparation method, effective component should be made big
Amount remains, while also requiring process simple, safe and environment-friendly.
Summary of the invention
The present invention provides a kind of preparation methods of Bitter Melon P.E, extract the single of balsam pear single component for existing
Property, Bitter Melon P.E obtained by one kind is extracted using balsam pear as raw material is proposed, solves the problems, such as above-mentioned background technique.
The first purpose of the invention is to provide a kind of preparation methods of Bitter Melon P.E, include the following steps：
Step 1, it selects without the hardship for being dried, crushed into 80~200 mesh after mildew, free from extraneous odour, the full fruit slice of fresh balsam pear
Melon powder, by balsam pear powder and water according to 1:5 mass ratio is uniformly mixed, and is subsequently placed in water at low temperature in extractor and is mentioned extracting to obtain and extracts
Extracting solution is filtered under diminished pressure to obtain filtrate by liquid, and concentrating filtrate to relative density is 1.10~1.15, obtains Bitter Melon P.E
Medicinal extract；
Step 2, Bitter Melon P.E medicinal extract will be obtained and carry out ceramic membrane separation using ceramic pot, and inhaled using macroreticular resin
It is attached, remove impurity；
Step 3, ferment is added in the Bitter Melon P.E medicinal extract after removal of impurities and carries out cold fermentation, carried out using specific embedding medium
Nanoscale embedding, smashes it through 80 meshes and obtains Bitter Melon P.E；
Step 4, it will be packed after the drying of obtained Bitter Melon P.E by 1 kilogram/bag.
Preferably, in the step 1, the temperature that water at low temperature proposes extraction is 10~45 DEG C.
Preferably, in the step 1, the condition of reduced pressure is 40 DEG C~60 DEG C, -0.08~-0.07Mpa.
Preferably, in the step 2, macroreticular resin is AB-8 type macroporous absorbent resin.
Preferably, in the step 3, ferment is papain, and embedding medium is pure glue.
The Bitter Melon P.E that the present invention is prepared is by every gram of finished product insulin content of measured by radioimmunoassay
5.10uIU/ml。
Second object of the present invention is protected by the Bitter Melon P.E of above method preparation in preparation with hypoglycemic, sugared
Application in terms of urine disease or health food.
Compared with prior art, the beneficial effects of the present invention are：
(1) present invention directly use water as solvent extraction contain the nutrition such as momordica saponins, bitter melon polysaccharide and bitter gourd polypeptide at
The Bitter Melon P.E divided, carries out cold fermentation using ferment, carries out nanoscale embedding using specific embedding medium, has sustained release work
With, and organic solvent is not used in process, product safety is pollution-free, extraction process is free from environmental pollution, extracting method
It is simple and quick, extraction cost is low.
(2) insulin content：The Bitter Melon P.E that the present invention prepares takes 1 gram to be placed in clean test tube, and 10 millis are added
It rises water for injection and every gram of Bitter Melon P.E is surveyed out by radioimmunology after completely dissolution in provincial hospital radio-immunity section
The content 5.10uIU/ml of finished product insulin.
Specific embodiment
In order to enable those skilled in the art to more fully understand, technical solution of the present invention is practiced, below with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Experimental method described in following each embodiments is unless otherwise specified conventional method.
Embodiment 1
The present embodiment provides a kind of preparation methods of Bitter Melon P.E, include the following steps：
Step 1, it selects without the balsam pear powder for being dried, crushed into 80 mesh after mildew, free from extraneous odour, the full fruit slice of fresh balsam pear,
By balsam pear powder and water according to 1:5 mass ratio is uniformly mixed, and is subsequently placed in water at low temperature in extractor and is mentioned and extracts to obtain extracting solution, will be mentioned
Liquid is taken to be filtered under diminished pressure to obtain filtrate, concentrating filtrate to relative density is 1.10~1.15, obtains Bitter Melon P.E medicinal extract；
Step 2, Bitter Melon P.E medicinal extract will be obtained and carry out ceramic membrane separation using ceramic pot, and inhaled using macroreticular resin
It is attached, remove impurity；
Step 3, ferment is added in the Bitter Melon P.E medicinal extract after removal of impurities and carries out cold fermentation, carried out using specific embedding medium
Nanoscale embedding, smashes it through 80 meshes and obtains Bitter Melon P.E；
Step 4, it will be packed after the drying of obtained Bitter Melon P.E by 1 kilogram/bag.
Wherein, in step 1, the temperature that water at low temperature proposes extraction is 15 DEG C.
Wherein, in step 1, the condition of reduced pressure is 45 DEG C, -0.07Mpa.
Wherein, in the step 2, macroreticular resin is AB-8 type macroporous absorbent resin.
Wherein, in the step 3, ferment is papain, and embedding medium is pure glue.
Embodiment 2
The present embodiment provides a kind of preparation methods of Bitter Melon P.E, include the following steps：
Step 1, it selects without the balsam pear powder for being dried, crushed into 200 mesh after mildew, free from extraneous odour, the full fruit slice of fresh balsam pear,
By balsam pear powder and water according to 1:5 mass ratio is uniformly mixed, and is subsequently placed in water at low temperature in extractor and is mentioned and extracts to obtain extracting solution, will be mentioned
Liquid is taken to be filtered under diminished pressure to obtain filtrate, concentrating filtrate to relative density is 1.10~1.15, obtains Bitter Melon P.E medicinal extract；
Step 2, Bitter Melon P.E medicinal extract will be obtained and carry out ceramic membrane separation using ceramic pot, and inhaled using macroreticular resin
It is attached, remove impurity；
Step 3, ferment is added in the Bitter Melon P.E medicinal extract after removal of impurities and carries out cold fermentation, carried out using specific embedding medium
Nanoscale embedding, smashes it through 80 meshes and obtains Bitter Melon P.E；
Step 4, it will be packed after the drying of obtained Bitter Melon P.E by 1 kilogram/bag.
Wherein, in step 1, the temperature that water at low temperature proposes extraction is 20 DEG C.
Wherein, in step 1, the condition of reduced pressure is 50 DEG C, -0.08Mpa.
Wherein, in the step 2, macroreticular resin is AB-8 type macroporous absorbent resin.
Wherein, in the step 3, ferment is papain, and embedding medium is pure glue.
Obtained Bitter Melon P.E is produced to embodiment 1 below to detect：
Inspection result
1. insulin content：The Bitter Melon P.E for taking 1 restraint to get ready is placed in clean test tube, and 10 milliliters of injections are added
Water surveys out every gram of Bitter Melon P.E finished product pancreas islet by radioimmunology after completely dissolution in provincial hospital radio-immunity section
The content 5.10uIU/ml of element.
2. momordica saponins content：9.73mg/ml (one perchloric acid colorimetric method of vanillic aldehyde).
3. polysaccharide identifies：Phenol-sulphate reagent reaction (positive).
4. polyoses content：13.41mg/ml (with phenol-sulfuric acid process measurement).
5. polypeptide identifies：This product 1ml is taken, is added few drops of ninhydrin solution, is heated, purple is shown, illustrates that this product is molten containing polypeptide
Liquid.
6. content of peptides：6.25mg/ml (Forint phenol method).
PH value is 2.92 (two VI H of annex of China's coastal port).
It is proved by animal biopsy, appropriate three weeks or so take compares the proliferation map of islet cells, there is apparent pancreas
Island repair.
It should be noted that involved in claims of the present invention when numberical range, it is thus understood that each numberical range
Any one numerical value can be selected between two endpoints and two endpoints, due to step method and the embodiment 1-2 phase of use
Together, it repeats in order to prevent, the present invention describes preferred embodiment, and once a person skilled in the art knows basic wounds
The property made concept, then additional changes and modifications may be made to these embodiments.So the following claims are intended to be interpreted as includes
Preferred embodiment and all change and modification for falling into the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (7)
1. a kind of preparation method of Bitter Melon P.E, which is characterized in that include the following steps：
Step 1, it selects without the balsam pear powder for being dried, crushed into 80~200 mesh after mildew, free from extraneous odour, the full fruit slice of fresh balsam pear,
By balsam pear powder and water according to 1:5 mass ratio is uniformly mixed, and is subsequently placed in water at low temperature in extractor and is mentioned and extracts to obtain extracting solution, will be mentioned
Liquid is taken to be filtered under diminished pressure to obtain filtrate, concentrating filtrate to relative density is 1.10~1.15, obtains Bitter Melon P.E medicinal extract；
Step 2, Bitter Melon P.E medicinal extract will be obtained and carry out ceramic membrane separation using ceramic pot, and use macroporous resin adsorption, removed
Decontamination；
Step 3, ferment is added in the Bitter Melon P.E medicinal extract after removal of impurities and carries out cold fermentation, carry out nanometer using specific embedding medium
Grade embedding, smashes it through 80 meshes and obtains Bitter Melon P.E；
Step 4, it will be packed after the drying of obtained Bitter Melon P.E by 1 kilogram/bag.
2. a kind of preparation method of Bitter Melon P.E according to claim 1, which is characterized in that in the step 1, low temperature
The temperature that water proposes extraction is 10~45 DEG C.
3. a kind of preparation method of Bitter Melon P.E according to claim 1, which is characterized in that in the step 1, decompression
The condition of concentration is 40 DEG C~60 DEG C, -0.08~-0.07Mpa.
4. a kind of preparation method of Bitter Melon P.E according to claim 1, which is characterized in that in the step 2, macropore
Resin is AB-8 type macroporous absorbent resin.
5. a kind of preparation method of Bitter Melon P.E according to claim 1, which is characterized in that in the step 3, ferment
For papain, embedding medium is pure glue.
6. the Bitter Melon P.E prepared method according to claim 1 passes through every gram of finished product insulin of measured by radioimmunoassay
Content is 5.10uIU/ml.
7. the Bitter Melon P.E prepared method according to claim 1 has hypoglycemic, diabetes or health food in preparation
The application of aspect.
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| CN113332380A (en) * | 2021-06-29 | 2021-09-03 | 浙江省人民医院 | A Chinese medicinal composition for treating diabetic nephropathy and preparation method thereof |
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