CN108850182A - A kind of preparation method of semen sojae atricolor protein functional beverage - Google Patents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
- A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
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Abstract
A kind of preparation method of semen sojae atricolor protein functional beverage belongs to food processing field, and semen sojae atricolor screened, cleaned by (1), rudiment processing；(2) semen sojae atricolor of rudiment is subjected to defibrination, filtering, enzymatic hydrolysis；(3) material after enzymatic hydrolysis is subjected to enzyme deactivation, defishying；(4) maltodextrin is added in the material after deodorant, after heating is boiled, is deployed with lactitol, citric acid, Co-Q10 and grape seed oil；(5) ultrasonic emulsification, homogenization are carried out to the material after allotment；(6) carry out that filling, sterilize to obtain semen sojae atricolor protein functional beverage product after homogeneous.The present invention is prepared using microwave continuous assistance enzymolysis and ultrasonic technique, converts small-molecular peptides for the high molecular weight protein in raw material, is conducive to human consumption and is absorbed；Co-Q10 and grape seed oil is added in allocation process, and stable emulsion oil-in-water is made, and guarantees that product nutrition is comprehensive, delicate mouthfeel, stability are good, extends storage phase；Do not add edible essence, preservative etc., guarantees the natural sex of product.
Description
Technical field
The invention belongs to food processing fields, relate generally to a kind of preparation method of semen sojae atricolor protein functional beverage.
Background technique
Nutritious black bean is abundant, has containing multiple nutritional components such as protein, fat, vitamin, microelements, while again
Various bioactivators, such as studies on black soybean pigment, semen sojae atricolor polysaccharide and isoflavones.Semen sojae atricolor has high protein, characteristic low in calories, egg
White matter content is up to 45% or more.Wherein high-quality protein is about higher by 1/4 or so than soya bean, occupies first of various beans, therefore
The good reputation of " king in beans " is won.Semen sojae atricolor is more conducive to the digestion and absorption of nutriment after germination, sprouts semen sojae atricolor and removes with black
Outside various nutritional ingredients possessed by beans, it is also enriched vitamin A, E, the functional materials such as riboflavin, melanin.Grape seed oil
Unsaturated fatty acid rich in, long-term consumption grape seed oil can reduce human serum cholesterol, effectively adjust the plant of people
Nervous function.In addition grape seed oil is also rich in vitamin E and proanthocyanidin, has stronger inoxidizability, has whitening to skin
Effect, and containing minerals and various fat-soluble and water soluble vitamins such as potassium needed by human, sodium, calcium, old man, baby can be met
The requirement of child and high altitude operation personnel etc..Co-Q10 has a variety of biological effects, and sufficient oxygen can be provided for cardiac muscle, prevents
Sudden heart disease；It delays senescence, protection skin：Prevent and treat chronic fatigue syndrome；Promote energy conversion, raising immune
Power promotes energy.Use lactitol that can meet the needs of diabetes patient and people to lose weight as sweetener.Enzymic catalytic reaction can make
Polyfunctional group substrate carries out mild, environmental-friendly conversion under the conditions of unshielded, and the selectivity with height, substrate are special
One property, enantioselectivity etc., but the speed of most enzyme digestion reactions is slower, thus industrial efficiency is affected, and microwave spoke
Auxiliary protein enzymatic hydrolysis is penetrated, enzymolysis time can be reduced to a few minutes from original a few hours；Some researches show that microwave-assisted ox bloods
Albumin enzymatic hydrolysis production polypeptide, can make the enzymolysis efficiency of protease basically reach 100%, enzymolysis time by conventional method 16h
It shorten to 20min.And the partial size for being significantly reduced lotion is ultrasonically treated to improve its storage stability and increase delicate mouthfeel degree.
But vegetable protein beverage unbalanced nutrition currently on the market, mouthfeel is single, and technique is cumbersome, and time-consuming, and production cost is higher.
Therefore, object of the present invention is to using rudiment semen sojae atricolor as raw material, using microwave continuous assistance enzymolysis and ultrasonic technique system
It is standby to form, small-molecular peptides are converted by the high molecular weight protein in raw material, is conducive to human consumption and absorbs；Coenzyme is added in allocation process
Stable emulsion oil-in-water is made in Q10 and grape seed oil, guarantees that product nutrition is comprehensive, delicate mouthfeel, stability are good, extends
Storage phase；Do not add edible essence, preservative etc. in process of producing product, guarantees the nutrient natural of product.
This method step is simple, adapts to large-scale production, and beverage nutritive value obtained is high, and smooth in taste is fine and smooth, storage
Stability is good.
Summary of the invention
The technical problem to be solved by the present invention is to overcome above-mentioned the deficiencies in the prior art, a kind of semen sojae atricolor protein function is provided
Property beverage preparation method, achieve the effect that be enriched with nutrition and Shelf-life, improve each of needed by human body in common beverages
Kind nutrient and functional components.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions：
A kind of preparation method of semen sojae atricolor protein functional beverage, this approach includes the following steps：
(1) semen sojae atricolor screened, cleaned, rudiment processing：Uniform, full, free from insect pests, epidermis are selected without breakage mildew
Complete semen sojae atricolor rinses after impurity elimination with 75% alcohol sterilizing, 1~2min, sterile water wash 3~5 times, impregnates 22 in room-temperature sterile water
~for 24 hours, surface moisture is drained, is sprouted to obtain germination semen sojae atricolor, institute with 4 layers of gauze covering after Culture basin middle berth cloth is uniform
The sprouting humidity 65~75% stated, 20~25 DEG C of Germination Temperature, 50~60h of sprout time；
(2) step (1) germination semen sojae atricolor is 1 by material-water ratio:7~10 enter coarse crushing in fiberizer, after 80 mesh colloid mills, obtain
Slurry 1；
(3) slurry 1 in step (2) is subjected to microwave continuous assistance enzymolysis, semen sojae atricolor quality 0.5%~1% is added
Papain, adjusting microwave power are 300~500W, and microwave temperature is 40~50 DEG C, and enzymatic hydrolysis pH is 8.5~9.0, and microwave is auxiliary
Helping enzymolysis time is 20~40min, must digest slurry 2；
(4) the enzymatic hydrolysis slurry 2 in step (3) does not apply heated under vacuum in heating in vacuum tank and boils 5~10min
Gradually adjustment vacuum degree extremely -0.07~-0.09MPa, vacuum 10~20min of deodorant obtains slurry 3 afterwards；
(5) slurry 3 in step (4) is added to 1% maltodextrin, after 20~30min is boiled in heating, with 1%~3%
Lactitol, 0.2~0.5% citric acid, 0.2~0.5% Co-Q10,1~3% grape seed oil allotment, obtain compound
Liquid；
(6) complex liquid in step (5) is ultrasonically treated, the ultrasonic power is 300~400W, ultrasonic time
5~10min；
(7) homogeneous：By the complex liquid after the ultrasound in step (6) at 15~20MPa 5~8min of homogeneous, obtain semen sojae atricolor egg
White functional beverage；
(8) filling, sterilizing：By pasteurization or high-temperature sterilization after the semen sojae atricolor protein functional beverage filling in step (7)
Obtain semen sojae atricolor protein functional beverage product.
Microwave continuous assistance enzymolysis technology, microwave power 480W are used in step (3), microwave temperature is 50 DEG C, enzyme
The solution time is 30min, digests pH8.6.
3% lactitol, 0.2% citric acid, 0.2% Co-Q10 and 2% grape seed oil are added in step (5)
Allotment.
Step is ultrasonically treated material in (6), ultrasonic power 400W, ultrasonic time 8min.
It is to contain protein, fat, vitamin, microelement etc. because nutritious black bean is abundant that semen sojae atricolor, which is selected, as raw material
Multiple nutritional components, while there are various bioactivators, such as studies on black soybean pigment, semen sojae atricolor polysaccharide and isoflavones again.Semen sojae atricolor tool
There are high protein, characteristic low in calories, protein content is up to 45% or more, and wherein high-quality protein is about higher by 1/4 left side than soya bean
It is right.Grape seed oil and Co-Q10 is added in allocation process, and stable emulsion oil-in-water is made using ultrasonic technique, can extend storage
The hiding phase.Grape seed oil unsaturated fatty acid rich in, can reduce human serum cholesterol, effectively adjust the vegetative nerve of people
Function.In addition it is also rich in vitamin E and proanthocyanidin, there is stronger inoxidizability, has whitening function to skin, and contain someone
The minerals such as potassium, sodium necessary to body, calcium and various fat-soluble and water soluble vitamin can meet old man, infant and high-altitude and make
The requirement of industry personnel etc..Co-Q10 has a variety of biological effects, and sufficient oxygen can be provided for cardiac muscle, prevents sudden heart disease；
It delays senescence, protection skin：Prevent and treat chronic fatigue syndrome；Improve immunity.Using microwave continuous assistance enzymolysis
Small-molecular peptides can be converted by the high molecular weight protein in raw material, be conducive to human consumption and absorb.This method step is simple, adapts to big rule
Mould production, beverage nutritive value obtained is high, and smooth in taste is fine and smooth, and storage stability is good.
Detailed description of the invention
Fig. 1 overall process route figure of the present invention.
Specific embodiment
The specific embodiment of the invention is described in detail below with reference to Fig. 1：
A kind of preparation method of semen sojae atricolor protein functional beverage, which is characterized in that include the following steps：
(1) semen sojae atricolor screened, cleaned, rudiment processing, selecting uniform, full, free from insect pests, epidermis without breakage mildew
Complete semen sojae atricolor rinses after impurity elimination with 75% alcohol sterilizing, 1~2min, sterile water wash 3~5 times, impregnates 22 in room-temperature sterile water
~for 24 hours, surface moisture is drained, is sprouted after Culture basin middle berth cloth is uniform with 4 layers of gauze covering, sprouting humidity 65~
75%, 20~25 DEG C of Germination Temperature, 50~60h of sprout time；
(2) step (1) germination semen sojae atricolor is 1 by material-water ratio:7~10 enter coarse crushing in fiberizer, after 80 mesh colloid mills, obtain
Slurry 1, it is spare；
(3) slurry 1 in step (2) is subjected to microwave continuous assistance enzymolysis, semen sojae atricolor quality 0.5%~1% is added
Papain, adjusting microwave power are 300~500W, and microwave temperature is 40~50 DEG C, and enzymolysis time is 20~40min, is obtained
Digest slurry 2；
(4) the enzymatic hydrolysis slurry 2 in step (3) does not apply heated under vacuum in heating in vacuum tank and boils 5~10min
Gradually for adjustment vacuum degree to -0.07~-0.09MPa, vacuum 10~20min of deodorant obtains slurry 3, spare afterwards；
(5) slurry 3 in step (4) is added to 1% maltodextrin, after 20~30min is boiled in heating, with 1%~3%
Lactitol, 0.2~0.5% citric acid, 0.2~0.5% Co-Q10,1~3% grape seed oil allotment, it is spare；
(6) material in step (5) is ultrasonically treated, ultrasonic power be 300~400W, ultrasonic time 5~
10min；
(7) by the material in step (6) at 15~20MPa 5~8min of homogeneous, obtain semen sojae atricolor protein functional beverage；
(8) pasteurization after the semen sojae atricolor protein functional beverage filling in step (7) or high-temperature sterilization are obtained into semen sojae atricolor albumen
Functional beverage finished product.
Embodiment 1：A kind of preparation method of semen sojae atricolor protein functional beverage, this approach includes the following steps：(1) by semen sojae atricolor
It screened, cleaned, rudiment processing, selected the complete semen sojae atricolor of uniform, full, free from insect pests, epidermis without breakage mildew, rinse impurity elimination
It is sterilized 1min with 75% alcohol afterwards, sterile water wash 3 times, impregnates 22h in room-temperature sterile water, drain surface moisture；It (2) will drip
It is 1 that semen sojae atricolor after solid carbon dioxide, which presses material-water ratio,:8 enter coarse crushing in fiberizer, after 80 mesh colloid mills, obtain slurry 1, spare；(3) enzyme
Solution：Slurry 1 is subjected to microwave continuous assistance enzymolysis, the papain of semen sojae atricolor quality 0.5% is added, adjusting microwave power is
480W, microwave temperature are 50 DEG C, and enzymatic hydrolysis pH is 8.6, and enzymolysis time 30min must digest slurry 2；(4) by slurry 2 in vacuum
Do not apply after heated under vacuum boils 5min in heating tank and gradually adjust vacuum degree extremely -0.07MPa, vacuum deodorant 10min,
Obtain slurry 3；(5) 1% maltodextrin is added into slurry 3, after 20min is boiled in heating, with 3% lactitol, 0.2% lemon
Lemon acid, 0.2% Co-Q10, the allotment of 2% grape seed oil；(6) material after allotment is ultrasonically treated, ultrasonic power
For 400W, ultrasonic time 8min；(7) by the material after ultrasound at 15MPa homogeneous 8min, obtain semen sojae atricolor protein functional beverage；
(8) by pasteurization after the semen sojae atricolor protein functional beverage filling after homogeneous or high-temperature sterilization obtain semen sojae atricolor protein functional beverage at
Product.
Embodiment 2：A kind of preparation method of semen sojae atricolor protein functional beverage, this approach includes the following steps：(1) by semen sojae atricolor
It screened, cleaned, rudiment processing, selected the complete semen sojae atricolor of uniform, full, free from insect pests, epidermis without breakage mildew, rinse impurity elimination
It is sterilized 2min with 75% alcohol afterwards, sterile water wash 3 times, impregnates 22h in room-temperature sterile water, surface moisture is drained, in Culture basin
It is sprouted after middle berth cloth is uniform with 4 layers of gauze covering, sprouting humidity 75%, 25 DEG C of Germination Temperature, sprout time 50h； (2)
First defibrination：Step (1) germination semen sojae atricolor is 1 by material-water ratio:10 enter coarse crushing in fiberizer, after 100 mesh colloid mills, obtain slurry 1,
It is spare；(3) it digests：Slurry 1 in step (2) is subjected to common water enzyme digestion, the papain of semen sojae atricolor quality 1% is added,
Adjusting hydrolysis temperature is 50 DEG C, and enzymatic hydrolysis pH is 8.6, and enzymolysis time 30min must digest slurry 2；(4) enzyme deactivation, deodorant：Step
(3) the enzymatic hydrolysis slurry 2 in, which does not apply in heating in vacuum tank after heated under vacuum boils 10 min, gradually adjusts vacuum degree
To -0.09MPa, vacuum deodorant 20min obtains slurry 3, spare；(5) emulsification allotment：Slurry 3 in step (4) is added 1%
Maltodextrin, heating boil 30min after, with 3% lactitol, 0.2% citric acid, 0.2% Co-Q10,2% grape
The allotment of seed oil, it is spare；(6) ultrasonic emulsification process：Material in step (5) is ultrasonically treated, ultrasonic power 400W, is surpassed
Sound time 8min；(7) homogeneous：By the material in step (6) at 15MPa homogeneous 8min, obtain semen sojae atricolor protein functional beverage；
(8) filling, sterilizing：Pasteurization after semen sojae atricolor protein functional beverage filling in step (7) or high-temperature sterilization are obtained into semen sojae atricolor egg
White functionality beverage product.
Embodiment 3：A kind of preparation method of semen sojae atricolor protein functional beverage, this approach includes the following steps：(1) by semen sojae atricolor
It screened, cleaned, rudiment processing, selected the complete semen sojae atricolor of uniform, full, free from insect pests, epidermis without breakage mildew, rinse impurity elimination
It is sterilized 2min with 75% alcohol afterwards, sterile water wash 3 times, impregnates 22h in room-temperature sterile water, surface moisture is drained, in Culture basin
It is sprouted after middle berth cloth is uniform with 4 layers of gauze covering, sprouting humidity 75%, 25 DEG C of Germination Temperature, sprout time 50h； (2)
First defibrination：Step (1) germination semen sojae atricolor is 1 by material-water ratio:10 enter coarse crushing in fiberizer, after 100 mesh colloid mills, obtain slurry 1,
It is spare；(3) it digests：Slurry 1 in step (2) is subjected to microwave continuous assistance enzymolysis, the pawpaw egg of semen sojae atricolor quality 1% is added
White enzyme, adjusting microwave power are 480W, and microwave temperature is 50 DEG C, and enzymatic hydrolysis pH is 8.6, and enzymolysis time 30min obtains enzymatic hydrolysis slurry
2；(4) enzyme deactivation, deodorant：Enzymatic hydrolysis slurry 2 in step (3) does not apply heated under vacuum in heating in vacuum tank and boils
Vacuum degree is gradually adjusted after 10min to -0.09MPa, vacuum deodorant 20min obtains slurry 3, spare；(5) emulsification allotment：By step
(4) 1% maltodextrin is added in slurry 3 in, after 30min is boiled in heating, with 3% lactitol, 0.2% citric acid,
0.2% Co-Q10,2% grape seed oil allotment, it is spare；(6) homogeneous：By the material in step (5) at 15MPa homogeneous
8min obtains semen sojae atricolor protein functional beverage；(7) filling, sterilizing：After the semen sojae atricolor protein functional beverage filling in step (6)
Pasteurization or high-temperature sterilization obtain semen sojae atricolor protein functional beverage product.
Embodiment 4：A kind of preparation method of semen sojae atricolor protein functional beverage, this approach includes the following steps：(1) by semen sojae atricolor
It screened, cleaned, rudiment processing, selected the complete semen sojae atricolor of uniform, full, free from insect pests, epidermis without breakage mildew, rinse impurity elimination
It is sterilized 2min with 75% alcohol afterwards, sterile water wash 3 times, impregnates 22h in room-temperature sterile water, surface moisture is drained, in Culture basin
It is sprouted after middle berth cloth is uniform with 4 layers of gauze covering, sprouting humidity 75%, 25 DEG C of Germination Temperature, sprout time 50h； (2)
First defibrination：Step (1) germination semen sojae atricolor is 1 by material-water ratio:10 enter coarse crushing in fiberizer, after 100 mesh colloid mills, obtain slurry 1,
It is spare；(3) it digests：Slurry 1 in step (2) is subjected to microwave continuous assistance enzymolysis, the pawpaw egg of semen sojae atricolor quality 1% is added
White enzyme, adjusting microwave power are 480W, and microwave temperature is 50 DEG C, and enzymatic hydrolysis pH is 8.6, and enzymolysis time 30min obtains enzymatic hydrolysis slurry
2；(4) enzyme deactivation, deodorant：Enzymatic hydrolysis slurry 2 in step (3) does not apply heated under vacuum in heating in vacuum tank and boils
Vacuum degree is gradually adjusted after 10min to -0.09MPa, vacuum deodorant 20min obtains slurry 3, spare；(5) emulsification allotment：By step
(4) 1% maltodextrin is added in slurry 3 in, after 30min is boiled in heating, with 3% lactitol, 0.2% citric acid,
0.2% Co-Q10,2% grape seed oil allotment, it is spare；(6) ultrasonic emulsification process：Material in step (5) is surpassed
Sonication, ultrasonic power 400W, ultrasonic time 8min；(7) homogeneous：By the material in step (6) at 15MPa homogeneous
8min obtains semen sojae atricolor protein functional beverage；(8) filling, sterilizing：After the semen sojae atricolor protein functional beverage filling in step (7)
Pasteurization or high-temperature sterilization obtain semen sojae atricolor protein functional beverage product.
Embodiment 5：Measure isoflavone content, protein content and the coenzyme of Examples 1 to 4 semen sojae atricolor protein functional beverage
Q10 embedding rate, specific step is as follows：
(1) isoflavone content measures：Above-mentioned four kinds of product vacuum freeze driers of sufficient amount are taken to freeze at -54 DEG C
Dry, 1.000 0g of sample is lyophilized in precise, is quantitatively adding 80% methanol, ultrasonic extraction 30min, with 80% methanol constant volume respectively
It to 50mL, shakes up, is centrifuged 20min (10 000r/min of revolving speed), gained supernatant is sample solution.Take genistein standard
Product 4mg is dissolved in dimethyl sulfoxide, and ultrasonic 20min is settled to 10mL and shakes up.Precision draws 1,0.5,0.125,0.05mL
This solution is respectively placed in 10mL volumetric flask, is added dimethyl sulfoxide constant volume, is shaken up.Using dimethyl sulfoxide as blank sample,
Absorbance is surveyed at 260nm.Using absorbance as ordinate, genistein concentration (μ g/mL) is abscissa, draws standard curve.Point
Not Xi Qu 1mL sample solution in 10mL volumetric flask, constant volume shakes up, and makees blank control with 80% methanol solution, at 260nm
The absorbance of sample, by the isoflavone content for each sample that standard curve and absorbance calculate.
(2) protein content determination：Measure the protein content in above-mentioned four kinds of products respectively with Kjeldahl's method.
(3) Co-Q10 embedding rate measures：The n-hexane of 3.0mL, oscillation mixing is added in the sample solution for taking 1.0mL respectively
5min is centrifuged under the conditions of centrifugal force 10000g after uniformly.Then it takes supernatant in 10mL volumetric flask constant volume, is surveyed at 450nm
Determine the light absorption value of Co-Q10.Coenzyme is calculated according to Co-Q10 standard bent (y=0.1245x+0.0045, R2=0.9999)
The mass concentration of Q10, i.e., the Co-Q10 mass concentration not being embedded (μ g/mL).Beta carotene is calculated with following formula to retain
Rate：
(4) analysis of experimental results：
The nutritive index of four kinds of samples
Sample 1~4 is respectively the product solution of Examples 1 to 4, and embodiment 1 does not carry out raw material rudiment processing, embodiment 2
The common water enzyme digestion that middle enzymatic hydrolysis stage uses, embodiment 3 do not carry out ultrasonic emulsification, embodiment 4 combine raw material rudiment processing,
Microwave-assisted enzymatic hydrolysis technology and ultrasonic emulsification technology.The sample of rudiment processing is not carried out according to the experimental result in above-mentioned table
Isoflavones and protein content are minimum in 1, illustrate that the isoflavones in product can be improved (research shows that isoflavones can prevent in rudiment
Menopause symptom is alleviated in the generation of cancer, cardiovascular disease and osteoporosis diseases, has anti-oxidant, antimycotic, anti-
Haemolysis and other effects) and protein content, improve the nutritive value of product；Co-Q10 embedding rate in the sample 3 not being ultrasonically treated
It is minimum, illustrate that the Co-Q10 embedding rate of product can be improved in ultrasound, wherein the height of Co-Q10 embedding rate can apparent product it is steady
Qualitative, Co-Q10 is oxidizable rotten, and embedding rate is higher, shows that the stability of product is better；Isoflavones in 4 product of sample
Content, protein content and Co-Q10 embedding rate highest illustrate that joint rudiment, microwave continuous enzymatic hydrolysis and ultrasonic emulsification technology can
Improve the nutritional quality and functional character of product.
Claims (4)
1. a kind of preparation method of semen sojae atricolor protein functional beverage, which is characterized in that this approach includes the following steps：
(1) semen sojae atricolor screened, cleaned, rudiment processing：Select uniform, full, free from insect pests, epidermis without breakage mildew it is complete
Semen sojae atricolor is rinsed after impurity elimination and is sterilized 1~2min with 75% alcohol, and sterile water wash 3~5 times, immersion 22 in room-temperature sterile water~
For 24 hours, surface moisture is drained, is sprouted to obtain germination semen sojae atricolor with 4 layers of gauze covering after Culture basin middle berth cloth is uniform, it is described
Sprouting humidity 65~75%, 20~25 DEG C of Germination Temperature, 50~60h of sprout time；
(2) step (1) germination semen sojae atricolor is 1 by material-water ratio:7~10 enter coarse crushing in fiberizer, after 80 mesh colloid mills, obtain slurry
1；
(3) slurry 1 in step (2) is subjected to microwave continuous assistance enzymolysis, the pawpaw of semen sojae atricolor quality 0.5%~1% is added
Protease, adjusting microwave power are 300~500W, and microwave temperature is 40~50 DEG C, and enzymatic hydrolysis pH is 8.5~9.0, microwave-assisted enzyme
The solution time is 20~40min, must digest slurry 2；
(4) the enzymatic hydrolysis slurry 2 in step (3) do not apply in heating in vacuum tank heated under vacuum boil after 5~10min by
Gradually adjustment vacuum degree extremely -0.07~-0.09MPa, vacuum 10~20min of deodorant obtains slurry 3；
(5) slurry 3 in step (4) is added to 1% maltodextrin, after 20~30min is boiled in heating, with 1%~3% cream
Sugar alcohol, 0.2~0.5% citric acid, 0.2~0.5% Co-Q10,1~3% grape seed oil allotment, obtain complex liquid；
(6) complex liquid in step (5) is ultrasonically treated, the ultrasonic power be 300~400W, ultrasonic time 5~
10min；
(7) homogeneous：By the complex liquid after the ultrasound in step (6) at 15~20MPa 5~8min of homogeneous, obtain semen sojae atricolor albumen function
It can property beverage；
(8) filling, sterilizing：Pasteurization after semen sojae atricolor protein functional beverage filling in step (7) or high-temperature sterilization are obtained black
Legumin functional beverage finished product.
2. a kind of preparation method of semen sojae atricolor protein functional beverage according to claim 1, which is characterized in that step (3)
Middle to use microwave continuous assistance enzymolysis technology, microwave power 480W, microwave temperature is 50 DEG C, enzymolysis time 30min, enzyme
Solve pH8.6.
3. a kind of preparation method of semen sojae atricolor protein functional beverage according to claim 1, which is characterized in that step (5)
It is middle that 3% lactitol, 0.2% citric acid, 0.2% Co-Q10 and the allotment of 2% grape seed oil is added.
4. a kind of preparation method of semen sojae atricolor protein functional beverage according to claim 1, which is characterized in that step (6)
In material is ultrasonically treated, ultrasonic power 400W, ultrasonic time 8min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112890206A (en) * | 2021-01-28 | 2021-06-04 | 麦和(广州)实业有限公司 | Black bean oligopeptide embedded substance and preparation method thereof |
| CN113925133A (en) * | 2021-11-05 | 2022-01-14 | 山西农业大学 | Method for preparing black soybean milk |
-
2018
- 2018-04-26 CN CN201810383688.4A patent/CN108850182A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112890206A (en) * | 2021-01-28 | 2021-06-04 | 麦和(广州)实业有限公司 | Black bean oligopeptide embedded substance and preparation method thereof |
| CN113925133A (en) * | 2021-11-05 | 2022-01-14 | 山西农业大学 | Method for preparing black soybean milk |
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