CN108850169A - A kind of Yoghourt and preparation method thereof - Google Patents
A kind of Yoghourt and preparation method thereof Download PDFInfo
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- CN108850169A CN108850169A CN201810554063.XA CN201810554063A CN108850169A CN 108850169 A CN108850169 A CN 108850169A CN 201810554063 A CN201810554063 A CN 201810554063A CN 108850169 A CN108850169 A CN 108850169A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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Abstract
The invention discloses a kind of Yoghourts and preparation method thereof, and wherein preparation method includes the following steps：The fresh celery of S1 adds water to be beaten, and obtains Celery Juice；S2, the Celery Juice is instilled dropwise in cyclodextrin solution and carries out ultrasound, continued ultrasound after being added dropwise to complete, mixed solution is filtered, then filtrate is concentrated, cooling is dried to obtain cyclodextrin inclusion compound；S3, bacteria suspension is instilled in chitosan solution dropwise, is continuously stirred until wet capsule is sufficiently formed, wet capsule is rinsed, finally obtains probiotic microcapsule by filtering；S4, the cyclodextrin inclusion compound, raw material milk, sweetener and stabilizer being mixed, homogeneous is sterilized, and it is cooling, the probiotic microcapsule is then added, refrigeration obtains the Yoghourt.The present invention utilizes chitosan imbedded probiotics, so that probiotics is in the ability enhancing for resisting extraneous poor environment, survival rate of the probio thalline in enteron aisle is also increased accordingly, so that Yoghourt is fresh-keeping more longlasting.
Description
Technical field
The present invention relates to dairy products, and in particular to a kind of Yoghourt and preparation method thereof.
Background technique
Yoghourt, through degreasing, partially skimmed or not degreasing, is sterilized, ferments, is stirred using ox (sheep) cream or reconstituted milk as main material
It mixes or does not stir, add or do not add dairy products made of other compositions, yoghurt-flavoured is sour-sweet fine and smooth, full of nutrition.In Yoghourt
Lactic acid bacteria can secrete the substance beneficial to human health when breeding in human body intestinal canal, the digestion power of people can be enhanced, promoted
Appetite, improves intestinal environment, and Yoghourt can also effectively improve the utilization rate of calcium, phosphorus in human body.It is continuous with living standard
Improve, and people's mental labour burden increasingly exacerbation, people more focuses on food heath-function and more trophism.To
Some nutrition classes or functional raw material are added in Yoghourt and producing novel Yoghourt is becoming a kind of development trend.
Celery vitamin A rich in, vitamin B1, vitamin B2, vitamin C and citrin, calcium, iron, the mines such as phosphorus
Content of material is also more, and in addition there are the ingredients such as protein, mannitol and food fiber.Also containing the celery of effective component in base of leaf
Sweet, the sweet lactone of fingered citron and volatile oil have the function of lowering blood pressure and blood fat, prevention and treatment atherosclerosis；It can also promote gastric juice
Secretion, increase appetite, often eat celery can stimulating gastrointestinal wriggle be conducive to defecation.
Celery is combined into production celery yoghurt with Yoghourt, product makees the double health for having celery and sour milk beverage concurrently
With nutritional ingredient is greatly improved than common sour milk.But in existing celery yoghurt the resistance, acid resistance of probiotics it is poor, to itching
Extremely sensitive, activity keeps more difficult.In addition, probiotics will reach effect good for health, it just must be with certain viable count
Amount can be colonized by gastric environment after reaching enteron aisle on intestinal mucosa.However, allow people bring down be in gastric juice compared with
Low pH value and oxidation-reduction potential, largely probiotics living waits probiotics to reach by that can be destroyed when gastric environment
After small intestine, the bile solutions of high concentration present in small intestine will cause being greatly lowered for probiotic active, be extremely difficult to it
Wholesome effect.
Summary of the invention
The main object of the present invention is to provide a kind of Yoghourt and preparation method thereof, it is intended to improve current celery yoghurt probiotics
Activity keeps difficult technical problem.
To achieve the above object, the present invention proposes a kind of preparation method of Yoghourt, includes the following steps：
S1, it is taken out after scalding the fresh celery of 25~30 parts by weight with hot water, adds water to be beaten, obtain Celery Juice；
S2, cyclodextrin solution is obtained by the cyclodextrin of 15~20 parts by weight is soluble in water, the Celery Juice is instilled dropwise
In the cyclodextrin solution and ultrasound is carried out, continues ultrasound after being added dropwise to complete, mixed solution is filtered, it is then that filtrate is dense
Contracting, cooling are dried to obtain cyclodextrin inclusion compound；
S3, the probiotics of 3~5 parts by weight is prepared into bacteria suspension with physiological saline；The chitosan of 5~8 parts by weight is molten
Solution adjusts pH value to faintly acid in hydrochloric acid solution, obtains chitosan solution and stirs, the bacteria suspension is instilled institute dropwise
It states in chitosan solution, continuously stirs until wet capsule is sufficiently formed, wet capsule is rinsed in filtering, and final acquisition probiotics is micro-
Capsule；
S4, by the cyclodextrin inclusion compound, the raw material milk of 50~60 parts by weight, the sweetener of 5~8 parts by weight and 0.4~
The stabilizer of 0.6 parts by weight mixes, homogeneous, sterilizes, cooling, and the probiotic microcapsule is then added, and ferments, and refrigeration obtains institute
State Yoghourt.
Preferably, the parts by volume of the physiological saline in step S3 is 1~1.5, and concentration is 0.8%~1%；The hydrochloric acid is molten
The concentration of liquid is 0.8~1.2mol/L, and parts by volume is 110~190.
Preferably, the time continuously stirred in the step S3 is at least 1h, the specific steps that wet capsule is rinsed
For：The wet capsule is rinsed 3~5 times with the physiological saline of sterilizing.
Preferably, the probiotics is lactobacillus acidophilus or Bifidobacterium BB01.
Preferably, the cyclodextrin is beta-cyclodextrin, gamma-cyclodextrin, hydroxypropyl-β-cyclodextrin, hydroxyethyl-β-cyclodextrin
Or methyl-B-cyclodextrin；The raw material milk is low fat, degreasing or whole milk；The sweetener includes white granulated sugar, fructose, sugar
Slurry, oligosaccharide and for one of sugar or a variety of；The stabilizer includes one of starch, pectin and gelatin or a variety of.
Preferably, the step S1 the specific steps are：95~100 DEG C of hot water of the fresh celery is scalded into 60~90s
After take out, be added be equivalent to 6~12 times of quality of the fresh celery water mashing, obtain Celery Juice.
Preferably, temperature ultrasonic in the step S2 be 20~30 DEG C, it is described continue after being added dropwise to complete ultrasound when
Between be 30~60min.
Preferably, the pressure of homogeneous is 25~30MPa in the step S4, and number is at least 2 times.
Preferably, the temperature sterilized in the step S4 is 98~100 DEG C, and the time is 5~6min；The temperature of the fermentation
It is 35~40 DEG C, the time is 6~8h, and the temperature of the refrigeration is 0~5 DEG C.
In addition, being prepared by any of the above-described preparation method, the Yoghourt the invention also provides a kind of Yoghourt
For yoghurt or acidophilus goat milk.
In technical solution of the present invention, Celery Juice is obtained after celery is beaten with water, then be slowly added dropwise into cyclodextrin solution,
Cyclodextrin inclusion compound is obtained using ultrasonic method, while being prepared by chitosan is wall material, probiotics is core material, and preparation probiotics is micro-
Probiotics is embedded in chitosan wall material by capsule, then mixes the cyclodextrin inclusion compound, raw material milk and ingredient, homogeneous,
The probiotic microcapsule is added, is fermented, refrigeration obtains the Yoghourt.The present invention utilizes natural macromolecular material chitosan packet
Probiotics is buried, so that probiotics is enhanced in terms of the ability for resisting extraneous poor environment, probio thalline is in enteron aisle
Survival rate also increase accordingly so that probiotics bacterial physical efficiency preferably plays its prebiotic effect, the Yoghourt being prepared is protected
It is fresh more longlasting.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
The structure shown according to these attached drawings obtains other attached drawings.
Fig. 1 is the flow chart of the method for preparing sour milk of the embodiment of the present invention.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
It in addition, the technical solution between each embodiment of the present invention can be combined with each other, but must be general with this field
Based on logical technical staff can be realized, it will be understood that when the combination of technical solution appearance is conflicting or cannot achieve this
The combination of technical solution is not present, also not the present invention claims protection scope within.
The present invention proposes a kind of preparation method of Yoghourt, as shown in Figure 1, including the following steps：
S1, it is taken out after the fresh celery of 25~30 parts by weight is scalded 60~90s with 95~100 DEG C of hot water, addition is equivalent to
The water of 6~12 times of quality of fresh celery is beaten, and obtains Celery Juice；
S2, cyclodextrin solution is obtained by the cyclodextrin of 15~20 parts by weight is soluble in water, Celery Juice is instilled to ring paste dropwise
It in smart solution and carries out ultrasound (temperature is 20~30 DEG C), continues 30~60min of ultrasound after being added dropwise to complete, by mixed solution mistake
Filter, then filtrate is concentrated, and cooling is dried to obtain cyclodextrin inclusion compound；
S3, by 1~1.5 parts by volume of the lactobacillus acidophilus of 3~5 parts by weight or Bifidobacterium BB01, concentration is 0.8%~
1% physiological saline is prepared into bacteria suspension；The chitosan of 5~8 parts by weight is dissolved in 110~190 parts by volume, concentration 0.8
In~1.2mol/L hydrochloric acid solution, and pH value is adjusted to faintly acid, obtain chitosan solution and stir, bacteria suspension is instilled dropwise
In chitosan solution, at least 1h is continuously stirred, until wet capsule is sufficiently formed, is filtered, it will be described wet with the physiological saline of sterilizing
Capsule rinses 3~5 times, finally obtains probiotic microcapsule；
S4, by cyclodextrin inclusion compound, the raw material milk of 50~60 parts by weight, the sweetener of 5~8 parts by weight and 0.4~0.6 weight
Measure part stabilizer mixing, homogeneous at least 2 times under 25~30MPa pressure, 98~100 DEG C at a temperature of sterilize 5~6min,
It is cooling, probiotic microcapsule is then added, 35~40 DEG C at a temperature of ferment 6~8h, 0~5 DEG C at a temperature of refrigerate
To Yoghourt.
Wherein, cyclodextrin is beta-cyclodextrin, gamma-cyclodextrin, hydroxypropyl-β-cyclodextrin, hydroxyethyl-β-cyclodextrin or first
Group-beta-cyclodextrin；Raw material milk is low fat, degreasing or whole milk；Sweetener includes white granulated sugar, fructose, syrup, oligosaccharide and for sugar
One of or it is a variety of；Stabilizer includes one of starch, pectin and gelatin or a variety of.
In addition, the invention also provides a kind of yoghurt prepared by the preparation method or acidophilus goat milks.
Probiotics can effectively improve human body in the function of immunology, in terms of nonspecific immune reaction, probiotics
Can effective activating macrophage so that its cell kill vigor on be improved；In specific immune response side
Face, probiotics can promote the increase of immunoglobulin.Probiotics also can be adjusted function of intestinal canal, pass through spontaneous metabolism
The immune defense function of enteron aisle is acted on and improved, guarantees the normal health function of enteron aisle.Probiotics has antianaphylaxis function, moreover it is possible to
Cholesterol is reduced, the balance of a variety of floras of the field planting in enteron aisle is maintained.In addition, probiotics can promote the wriggling of enteron aisle, thus
Residence time of the carcinogen in enteron aisle is reduced, it can be with prophylactic treatment hypertension, protection liver, treatment constipation etc..But benefit
The numerous wholesome treatments of raw bacterium and the acquisition of healthcare function are also required to some conditions, that is, must have quite sufficient
Probiotics quantity on enteron aisle.
However tradition application upper Bacillus acidi lactici and Bifidobacterium etc. probiotics resistance during the growth process, acid resistance compared with
Poor, extremely sensitive to oxygen, activity keeps more difficult.In addition, probiotics will reach effect good for health, it just must be with certain
Number of viable can be colonized by gastric environment after reaching enteron aisle on intestinal mucosa.However, allowing people to bring down is stomach
Lower pH value and oxidation-reduction potential in liquid, largely probiotics living is waited prebiotic by that can be destroyed when gastric environment
After bacterium reaches small intestine, the bile solutions of high concentration present in small intestine will cause being greatly lowered for probiotic active, be difficult
Reach its wholesome effect.
Chitosan is also known as chitosan, is to be obtained by the chitin that nature is widely present by deacetylation
, chitosan is natural macromolecular material, and good biocompatibility has no toxic side effect, and has active hydroxyl and amino, they have
There is stronger chemical reaction ability, the present invention embeds probiotics using chitosan as wall material, can play to probiotics
Effect is effectively protected, protects it from the influence of poor environment.After taking probiotic products, more probiotics viable bacterias is allowed to arrive
Up to enteron aisle, to play the role of wholesome.
The outer rim of cyclodextrin is hydrophilic and inner cavity is hydrophobic, thus it can provide a hydrophobic binding site as enzyme, makees
Based on the various objects appropriate of envelope, such as organic molecule, inorganic ions and gas molecule.The hydrophobic and external parent in its inner cavity
The characteristic of water make its can according to Van der Waals force, hydrophobic interaction power, the intermolecular matching effect of Subjective and Objective etc. with it is many organic
Inclusion compound is formed with inorganic molecule.And flavor molecules, insoluble diedairy fiber, vitamin in celery etc. can enter in cyclodextrin
Chamber forms inclusion compound, can cover the flavor of celery, the dissolubility for the ingredient that has additional nutrients, so that the fermentation of Yoghourt is more smooth, increases
The nutrient content for having added celery in Yoghourt avoids vitamin etc. affected by environment.Therefore Yoghourt of the invention is fine and smooth and taste is good,
Have both the double nutrition of celery and Yoghourt.
Certainly in other embodiments, in addition to preparing cyclodextrin inclusion compound with ultrasonic method, can also with solution stirring method or
The preparation of person's polishing.Such as Celery Juice is slowly dropped into cyclodextrin solution under stirring, 1h is stirred at room temperature, overnight, takes out
It filters and washs saturate precipitating with a small amount of dehydrated alcohol, vacuum drying obtains inclusion compound.Or by cyclodextrin solution and Celery Juice
After mixing, it is fully ground 1h, decompression filters, and filter cake is dried to constant weight under 50 DEG C of ovens to get inclusion compound.But solution stirs
Mix process and polishing embedding effect are not that very well, under relatively, when ultrasonic wave is propagated in a liquid, can generate intensifying for energy
And burst, i.e., ultrasonic " empty verbiage effect ", it can discharge huge energy, shock wave be formed, instead of stirring action to product
It is embedded, ultrasonic method embedding effect is more preferable, the time is short, easy to control and easy to operate.
Cyclodextrin can also be complexed with the cholesterol in raw material milk, therefore, raw material milk can slowly be instilled to ring paste dropwise
In smart aqueous solution and carry out ultrasonic (temperature：20~30 DEG C), continue ultrasound after being added dropwise to complete, is then centrifuged mixed liquor, mistake
Filter, cooling are dried to obtain cyclodextrin-cholesterol complex compound.Wherein filtered filtrate is the raw material milk of Cholesterol removal,
Step S4 is carried out again, it can obtains the Yoghourt of removing cholesterol, the crowd of suitable too high cholesterol drinks.
Therefore, Yoghourt of the invention is in good taste, has both the double nutrition of celery and Yoghourt, and makes probio thalline in enteron aisle
In survival rate also increase accordingly so that probiotics bacterial physical efficiency preferably plays its prebiotic effect, Yoghourt is fresh-keeping more longlasting.
Embodiment 1
The Yoghourt of the present embodiment, preparation method includes the following steps：
S1, it is taken out after the fresh celery of 30g is scalded 60s with 100 DEG C of hot water, the water mashing of 150ml is added, obtains celery
Juice；
S2, cyclodextrin solution is obtained by the beta-cyclodextrin of 15g is soluble in water, it is molten that Celery Juice is slowly instilled to cyclodextrin dropwise
It in liquid and carries out ultrasound (temperature is 25 DEG C), continues ultrasound 40min after being added dropwise to complete, mixed solution is filtered, is then utilized
Filtrate is concentrated Rotary Evaporators, and cooling is dried to obtain cyclodextrin inclusion compound；
S3, the freeze-dried vaccine powder of lactobacillus acidophilus is inoculated in 121 DEG C of sterilizing 15min MRS broth bouillons after cooling
It is activated three times, it is preceding twice with 5% inoculum concentration, it cultivates under 37 DEG C of constant temperatures for 24 hours, is finally existed with 3% inoculum concentration
18h is cultivated under the conditions of identical temperature, it is finally that the strain cryo-conservation after activation is spare, it will activate to the acidophilus of the third generation
Lactobacillus is centrifuged (1000r, 12min) and collects bacterium mud, takes the bacterium mud 1ml of 3g lactobacillus acidophilus, the physiology salt that concentration is 0.8%
Water is prepared into bacteria suspension；
It is in 0.8mol/L hydrochloric acid solution, to after completely dissolution, use 1mol/l that 5g chitosan, which is dissolved in 110mL concentration,
It is 5 that NaOH solution and deionized water, which adjust chitosan solution pH, obtains chitosan solution, with hand gun by bacteria suspension with by
The mode instilled, is expressed to and is placed in the chitosan solution on magnetic stirring apparatus, continuously stir 1h, until the abundant shape of wet capsule
At, the wet capsule of formation is filtered with gauze, then rinsed wet capsule 3 times with the physiological saline of sterilizing, it is final to obtain benefit
Raw bacteria microcapsule；
S4, cyclodextrin inclusion compound, 50g lowfat milk, 5g white granulated sugar, fructose and 0.4g gelatin are mixed, in 25MPa pressure
Lower homogeneous 2 times, 98 DEG C at a temperature of sterilize 5min, it is cooling, probiotic microcapsule is then added, 35 DEG C at a temperature of ferment
6h obtains the Yoghourt of the present embodiment in 5 DEG C of at a temperature of refrigeration.
Embodiment 2
The Yoghourt of the present embodiment, preparation method includes the following steps：
S1, it is taken out after the fresh celery of 25g is scalded 90s with 95 DEG C of hot water, the water mashing of 300ml is added, obtains Celery Juice；
S2, cyclodextrin solution is obtained by the gamma-cyclodextrin of 20g is soluble in water, Celery Juice is slowly instilled into cyclodextrin dropwise
It in solution and carries out ultrasound (temperature is 30 DEG C), continues ultrasound 60min after being added dropwise to complete, mixed solution is filtered, it is then sharp
Filtrate is concentrated with Rotary Evaporators, cooling is dried to obtain cyclodextrin inclusion compound；
S3, the freeze-dried vaccine powder of Bifidobacterium BB01 is inoculated in 120 DEG C of sterilizing 18min MRS broth bouillons after cooling
It is middle to be activated three times, it is preceding twice with 5% inoculum concentration, it is cultivated under 37 DEG C of constant temperatures for 24 hours, finally with 3% inoculum concentration
18h is cultivated under the conditions of identical temperature, it is finally that the strain cryo-conservation after activation is spare, it is thermophilic to the third generation by having activated
Lactobacillus lactis is centrifuged (1000r, 15min) and collects bacterium mud, takes the bacterium mud 1.5ml of 5g Bifidobacterium BB01, the life that concentration is 1%
Reason salt water is prepared into bacteria suspension；
It is in 1.2mol/L hydrochloric acid solution, to after completely dissolution, use 1mol/l that 8g chitosan, which is dissolved in 190mL concentration,
It is 4.5 that NaOH solution and deionized water, which adjust chitosan solution pH, obtains chitosan solution, with hand gun by bacteria suspension with
The mode instilled dropwise is expressed to and is placed in the chitosan solution on magnetic stirring apparatus, continuously stirs 1.2h, until wet capsule fills
Divide and formed, be filtered the wet capsule of formation with gauze, then rinsed wet capsule 3 times with the physiological saline of sterilizing, finally obtains
Obtain probiotic microcapsule；
S4, cyclodextrin inclusion compound, 60g whole milk, 8g white granulated sugar, syrup, oligosaccharide and 0.6g starch are mixed,
Homogeneous 2 times under 30MPa pressure, 100 DEG C at a temperature of sterilize 6min, it is cooling, probiotic microcapsule is then added, at 40 DEG C
At a temperature of ferment 8h, obtain the Yoghourt of the present embodiment in 3 DEG C of at a temperature of refrigeration.
Embodiment 3
The Yoghourt of the present embodiment, preparation method includes the following steps：
S1, it is taken out after the fresh celery of 28g is scalded 70s with 98 DEG C of hot water, the water mashing of 280ml is added, obtains Celery Juice；
S2, cyclodextrin solution is obtained by the hydroxypropyl-β-cyclodextrin of 18g essence is soluble in water, Celery Juice is slowly dripped dropwise
Enter in cyclodextrin solution and carry out ultrasound (temperature is 28 DEG C), continues ultrasound 50min after being added dropwise to complete, by mixed solution mistake
Then filter filtrate is concentrated using Rotary Evaporators, cooling is dried to obtain cyclodextrin inclusion compound；
S3, the freeze-dried vaccine powder of lactobacillus acidophilus is inoculated in 120 DEG C of sterilizing 17min MRS broth bouillons after cooling
It is activated three times, it is preceding twice with 5% inoculum concentration, it cultivates under 37 DEG C of constant temperatures for 24 hours, is finally existed with 3% inoculum concentration
18h is cultivated under the conditions of identical temperature, it is finally that the strain cryo-conservation after activation is spare, it will activate to the acidophilus of the third generation
Lactobacillus is centrifuged (1000r, 12min) and collects bacterium mud, takes the bacterium mud 1.2ml of 4g lactobacillus acidophilus, the physiology that concentration is 0.9%
Salt water is prepared into bacteria suspension；
It is in 0.9mol/L hydrochloric acid solution, to after completely dissolution, use 1mol/l that 7g chitosan, which is dissolved in 150mL concentration,
It is 5.2 that NaOH solution and deionized water, which adjust chitosan solution pH, obtains chitosan solution, with hand gun by bacteria suspension with
The mode instilled dropwise is expressed to and is placed in the chitosan solution on magnetic stirring apparatus, continuously stirs 1h, until wet capsule is abundant
It is formed, is filtered the wet capsule of formation with gauze, then rinsed wet capsule 3 times with the physiological saline of sterilizing, it is final to obtain
Probiotic microcapsule；
S4, cyclodextrin inclusion compound, 55g degreasing goat milk, 7g D-sorbite (for one of sugar) and 0.5g pectin are mixed,
Homogeneous 3 times under 28MPa pressure, 99 DEG C at a temperature of sterilize 5min, it is cooling, probiotic microcapsule is then added, at 37 DEG C
At a temperature of ferment 7h, obtain the Yoghourt of the present embodiment in 4 DEG C of at a temperature of refrigeration.
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all in the present invention
Design under, using equivalent transformation made by description of the invention, or directly/to be used in other related technical areas equal indirectly
It is included within the scope of the present invention.
Claims (10)
1. a kind of preparation method of Yoghourt, which is characterized in that include the following steps：
S1, it is taken out after scalding the fresh celery of 25~30 parts by weight with hot water, adds water to be beaten, obtain Celery Juice；
S2, obtain cyclodextrin solution for the cyclodextrin of 15~20 parts by weight is soluble in water, the Celery Juice is instilled dropwise described in
In cyclodextrin solution and ultrasound is carried out, continues ultrasound after being added dropwise to complete, mixed solution is filtered, then filtrate is concentrated, it is cold
But it is dried to obtain cyclodextrin inclusion compound；
S3, the probiotics of 3~5 parts by weight is prepared into bacteria suspension with physiological saline；The chitosan of 5~8 parts by weight is dissolved in
In hydrochloric acid solution, and pH value is adjusted to faintly acid, obtain chitosan solution and stir, the bacteria suspension is instilled into the shell dropwise
In glycan solution, continuously stir until wet capsule is sufficiently formed, wet capsule is rinsed, finally obtains probiotic microcapsule by filtering；
S4, by the cyclodextrin inclusion compound, the raw material milk of 50~60 parts by weight, the sweetener of 5~8 parts by weight and 0.4~0.6 weight
The stabilizer mixing of part is measured, homogeneous is sterilized, and it is cooling, the probiotic microcapsule is then added, ferments, refrigeration obtains the acid
Milk.
2. such as preparation method as described in claim 1, which is characterized in that the parts by volume of the physiological saline in the step S3 is
1~1.5, concentration is 0.8%~1%；The concentration of the hydrochloric acid solution is 0.8~1.2mol/L, and parts by volume is 110~190.
3. preparation method as described in claim 1, which is characterized in that the time continuously stirred in the step S3 is at least
1h, it is described by wet capsule rinse the specific steps are：The wet capsule is rinsed 3~5 times with the physiological saline of sterilizing.
4. preparation method as described in claim 1, which is characterized in that the probiotics is lactobacillus acidophilus or Bifidobacterium
BB01。
5. preparation method as described in claim 1, which is characterized in that the cyclodextrin is beta-cyclodextrin, gamma-cyclodextrin, hydroxyl
Propyl-beta-cyclodextrin, hydroxyethyl-β-cyclodextrin or methyl-B-cyclodextrin；The raw material milk is low fat, degreasing or whole milk；
The sweetener includes white granulated sugar, fructose, syrup, oligosaccharide and for one of sugar or a variety of；The stabilizer include starch,
One of pectin and gelatin are a variety of.
6. preparation method as described in claim 1, which is characterized in that the step S1 the specific steps are：By the fresh celery
Dish takes out after scalding 60~90s with 95~100 DEG C of hot water, and the water mashing for being equivalent to 6~12 times of quality of the fresh celery is added, obtains
To Celery Juice.
7. preparation method as described in claim 1, which is characterized in that ultrasonic temperature is 20~30 DEG C in the step S2,
The time for continuing ultrasound after being added dropwise to complete is 30~60min.
8. preparation method as described in claim 1, which is characterized in that the pressure of homogeneous is 25~30MPa in the step S4,
Number is at least 2 times.
9. such as preparation method according to any one of claims 1 to 8, which is characterized in that the temperature sterilized in the step S4
It is 98~100 DEG C, the time is 5~6min；The temperature of the fermentation is 35~40 DEG C, and the time is 6~8h, the temperature of the refrigeration
It is 0~5 DEG C.
10. a kind of Yoghourt, which is characterized in that the preparation of Yoghourt preparation method as described in any one of claim 1~9
It obtains, the Yoghourt is yoghurt or acidophilus goat milk.
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| CN113040272A (en) * | 2021-02-24 | 2021-06-29 | 深圳净拓生物科技有限公司 | Formula milk for aseptic animals and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113040272A (en) * | 2021-02-24 | 2021-06-29 | 深圳净拓生物科技有限公司 | Formula milk for aseptic animals and preparation method thereof |
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