CN108849493A - The raising technology of triploid Radix Glycyrrhizae - Google Patents
The raising technology of triploid Radix Glycyrrhizae Download PDFInfo
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- CN108849493A CN108849493A CN201710333657.3A CN201710333657A CN108849493A CN 108849493 A CN108849493 A CN 108849493A CN 201710333657 A CN201710333657 A CN 201710333657A CN 108849493 A CN108849493 A CN 108849493A
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- Prior art keywords
- radix glycyrrhizae
- triploid
- diploid
- raising technology
- autotetraploid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the raising technologies of triploid Radix Glycyrrhizae.It is characterized in that the mode of this technology genetic breeding, then existing diploid Radix Glycyrrhizae is hybridized, transformation makes its medicinal effects yield and quality all be greatly improved at triploid Radix Glycyrrhizae using colchicine mutagenesis at autotetraploid with diploid Radix Glycyrrhizae.The technology is including 1, by existing diploid Radix Glycyrrhizae mutagenesis at autotetraploid；2, then autotetraploid Radix Glycyrrhizae is hybridized with diploid Radix Glycyrrhizae, transformation is at triploid Radix Glycyrrhizae.
Description
Technical field
The present invention relates to Chinese medicines to expand breeding planting technology field, and in particular to the raising technology of triploid Radix Glycyrrhizae.
Background technique
Traditional Chinese medicine is the quintessence of Chinese culture in China, is the rarity of the magnificent culture of the Chinese nation.The utilization and development of Chinese medicine are to enter
Modernization stage, large parts of generic medicinal plants all realize artificial growth substantially（Cultivation）, Radix Glycyrrhizae is existing 1800 around the Minxian County of Gansu
The artificial growth history of many years.For many years plant, variety deterioration, pest and disease damage is more, planting process using a large amount of high poisons chemical fertilizer and
Pesticide makes quality of medicinal material decline or harmful.It is higher and higher to healthy attention degree with being gradually increased for people's living standard,
Increasing to the demand of traditional Chinese medicine, health product, country pays much attention to Developing TCM cause and scientific and technological Huinong, Chinese medicine
Demand rapidly increasing, medicinal herb grower urgently increase income makes the life better, while centering quality of medicinal material require also further increase.
People's health and market just propose the demand improved the breed.
Recent domestic is quickly grown in terms of plant genetics and breeding research, is concentrated mainly on China, Japan and India.
Induced mutations breeding is one of to cultivate the plant variety with merit, improve quality, the effective way for improving yield.China
The triploid Radix Salviae Miltiorrhizae single plant maximum output of incubation is more than 2000 grams, and the diploid Radix Salviae Miltiorrhizae single plant root yield of domestic plantation at present
Averagely only have 200 to 300 grams, 7-10 times of output increased.The camomile tetraploid kind of Japanese successful incubation, azulenes ester content
It is 1.2 times of diploid, quirk product is 2.3 times of diploid.The triploid plant of the most representative artificial breeding in China is
Triploid seedless watermelon.
Triploid plant generally have root, stem, leaf, flower, fruit giantism, resistance is strong, and available component content is high
Etc. characteristics, this is exactly the high-quality of medicinal material, the purpose that reaches desired by SOYBEAN IN HIGH-YIELD BREEDING, therefore the triploid Breeding of medicinal plant has
Special application value and huge yield potential.
Summary of the invention
The purpose of the present invention is to provide the raising technologies of triploid Radix Glycyrrhizae to be bred out and is higher than with the mode of genetic breeding
The triploid Radix Glycyrrhizae of its ancestors.Specially by existing diploid Radix Glycyrrhizae using colchicine mutagenesis at autotetraploid, then with
From the diploid Radix Glycyrrhizae hybridization that different regions are wild or cultivate, transformation makes its medicinal effects yield and matter at triploid Radix Glycyrrhizae
Amount is all greatly improved.In order to develop the plantation of genuine main producing region Chinese medicine Radix Glycyrrhizae, so that it is maintained genuineness, have
Stronger resistance, greatly improves yield and quality, provides the licorice medicinal materials of high quality, improves farmers' income.
In order to realize the purpose, the technical solution used in the present invention includes the following steps：
1, induced mutations is carried out using colchicine.Colchicine is a kind of microtubule depolymerization agent, is usually used in inducing plant chromosome
It doubles.But the tubulin in various plants and different tissues is different from colchicine binding ability, thus handle when it is noted that
Grasp the concentration of colchicine.This research is used for the suitable colchicine mutagenesis concentration 10~30% of Radix Glycyrrhizae chromosome doubling.
2, mutagenesis goes out tetraploid Radix Glycyrrhizae.Mitotic cell is being carried out with Radix Glycyrrhizae with the colchicine of above-mentioned concentration to contact
When, first in conjunction with tubulin heterodimer, to block tubulin to be assembled into micro-pipe and cause original microtubule depolymerization, make
Function relevant to micro-pipe is hindered and loses in cell, cannot form spindle fiber, hinders the later cell division of mid-term
Process.When colchicine is washed off, after cell restores proper splitting function, chromosome number increases 1 times, generates chromosome number
The core doubled cultivates tetraploid Radix Glycyrrhizae.
3, triploid Radix Glycyrrhizae plant is obtained.Hybridized by tetraploid Radix Glycyrrhizae and diploid Radix Glycyrrhizae, obtains triploid Radix Glycyrrhizae and plant
Strain.The homologue of triploid is three groups, and in the later period for carrying out meiosis, chromosome is unable to complete equilibrium assignment,
Normal gamete can not be formed, as a result, result in the extremely unbalanced gamete of chromosome number, the height of reproduction cell is caused to lose
It educates, generates without seed phenomenon.
4, ideal triploid Radix Glycyrrhizae kind is selected.
Specific embodiment
Embodiment
In the best suitable planting region of Radix Glycyrrhizae, the preferable Field of Glycyrrhiza uralensis of growing way is selected, in Radix Glycyrrhizae florescence, the autumn for being 15% with concentration
Narcissus element is carrying out mitotic cell with Radix Glycyrrhizae and is contacting, and makes it first in conjunction with tubulin heterodimer, to hinder
Disconnected tubulin is assembled into micro-pipe and causes original microtubule depolymerization, and function relevant to micro-pipe in cell is made to be hindered or lose
It loses, spindle fiber cannot be formed, hinder the later cell division process of mid-term.When colchicine is washed off, cell restores normal
After splitting function, chromosome number increases 1 times, generates the core that chromosome number doubles, cultivates tetraploid Radix Glycyrrhizae, waits its maturation
Receive seed.
Second year nursery, the transplanting of third year is tetraploid Radix Glycyrrhizae, is hybridized to the florescence with existing diploid Radix Glycyrrhizae, is received after mature
Seed, as triploid Glycyrrhiza Seeds.
It cultivates identical as existing diploid Radix Glycyrrhizae.
Claims (4)
1. the raising technology of triploid Radix Glycyrrhizae is to breed existing diploid Radix Glycyrrhizae at triploid Radix Glycyrrhizae.
2. the raising technology of triploid Radix Glycyrrhizae as described in claim 1, it is characterised in that：The first step, by existing diploid Radix Glycyrrhizae
Mutagenesis is at autotetraploid.
3. the raising technology of triploid Radix Glycyrrhizae as described in claim 1, it is characterised in that：Second step, by autotetraploid Radix Glycyrrhizae
With different regions it is wild or cultivation diploid Radix Glycyrrhizae hybridize, transformation is at triploid Radix Glycyrrhizae.
4. the raising technology of triploid Radix Glycyrrhizae as described in claim 1, it is characterised in that：It carries out tetraploid mutagenesis and uses colchicum
Element, concentration is 10~30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710333657.3A CN108849493A (en) | 2017-05-12 | 2017-05-12 | The raising technology of triploid Radix Glycyrrhizae |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710333657.3A CN108849493A (en) | 2017-05-12 | 2017-05-12 | The raising technology of triploid Radix Glycyrrhizae |
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| CN108849493A true CN108849493A (en) | 2018-11-23 |
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| CN201710333657.3A Pending CN108849493A (en) | 2017-05-12 | 2017-05-12 | The raising technology of triploid Radix Glycyrrhizae |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030172414A1 (en) * | 2002-03-05 | 2003-09-11 | Xingping Zhang | Enhanced pollenizer and method for increasing seedless watermelon yield |
| CN101124887A (en) * | 2007-09-28 | 2008-02-20 | 南开大学 | Method for cultivating triploid of red sage root |
| CN101390492A (en) * | 2008-11-06 | 2009-03-25 | 南开大学 | Natural hybridization seed-production method of triploid radix salvia miltiorrhizae using purple gene as label character |
| CN101690461A (en) * | 2009-11-02 | 2010-04-07 | 四川农业大学 | Method for preparing triploid plants |
| CN101932228A (en) * | 2008-02-01 | 2010-12-29 | 瑞克斯旺种苗集团公司 | Seedless triploid eggplant |
| CN104521760A (en) * | 2015-01-14 | 2015-04-22 | 甘肃农业大学 | Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture |
| CN108849470A (en) * | 2017-05-12 | 2018-11-23 | 王元龙 | The raising technology of triploid rheum officinale |
-
2017
- 2017-05-12 CN CN201710333657.3A patent/CN108849493A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030172414A1 (en) * | 2002-03-05 | 2003-09-11 | Xingping Zhang | Enhanced pollenizer and method for increasing seedless watermelon yield |
| CN101124887A (en) * | 2007-09-28 | 2008-02-20 | 南开大学 | Method for cultivating triploid of red sage root |
| CN101932228A (en) * | 2008-02-01 | 2010-12-29 | 瑞克斯旺种苗集团公司 | Seedless triploid eggplant |
| CN101390492A (en) * | 2008-11-06 | 2009-03-25 | 南开大学 | Natural hybridization seed-production method of triploid radix salvia miltiorrhizae using purple gene as label character |
| CN101690461A (en) * | 2009-11-02 | 2010-04-07 | 四川农业大学 | Method for preparing triploid plants |
| CN104521760A (en) * | 2015-01-14 | 2015-04-22 | 甘肃农业大学 | Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture |
| CN108849470A (en) * | 2017-05-12 | 2018-11-23 | 王元龙 | The raising technology of triploid rheum officinale |
Non-Patent Citations (3)
| Title |
|---|
| 沈德绪等: "《园艺植物遗传学》", 30 November 1985, 农业出版社 * |
| 王丽艳等: "秋水仙碱诱导甘草四倍体的研究", 《激光生物学报》 * |
| 黄华孙: "《中国橡胶树育种五十年》", 30 November 2005, 中国农业出版社 * |
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Application publication date: 20181123 |