CN108841897A - It is a kind of for producing the rhodozyma culture method of trehalose - Google Patents
It is a kind of for producing the rhodozyma culture method of trehalose Download PDFInfo
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- CN108841897A CN108841897A CN201810774256.6A CN201810774256A CN108841897A CN 108841897 A CN108841897 A CN 108841897A CN 201810774256 A CN201810774256 A CN 201810774256A CN 108841897 A CN108841897 A CN 108841897A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The present invention relates to Yeast Cultivation technical fields, specifically disclose a kind of rhodozyma culture method for producing trehalose and include the following steps：Step 1, strain preparation, step 2, bacterial strain screening, step 3, culture medium preparation, step 4, Yeast Cultivation, step 5, content of trehalose measurement.The present invention overcomes the deficiencies in the prior art, choose the high yeast strain of content of trehalose, and culture increases the content of trehalose in yeast cells under certain condition, improves production efficiency with the yeast cultivated in this way to produce trehalose, reduces production cost.
Description
Technical field
The present invention relates to Yeast Cultivation technical fields, particularly belong to a kind of for producing the rhodozyma culture method of trehalose.
Background technique
Trehalose is a kind of stabilization irreducibility disaccharide obtained by biofermentation technique from cornstarch, is highly soluble in
Water, trehalose can help organism to fight various harsh environment to keep the bioactivity of organism, and such as dry, frost is high
Temperature, hyperosmotic state etc.；There are such evaluation in scientific circles to trehalose, and " for many life entities, the being and not being of trehalose is meaned
Life or death ", therefore be known as the title of " sugar of life ", highlighted trehalose to the importance of organism.
Existing trehalose mainly extracts production from yeast, and the content of trehalose of different yeast has differences, different
Under the conditions of the content of trehalose of yeast cultivated it is also not identical.
Summary of the invention
The rhodozyma culture method that the object of the present invention is to provide a kind of for producing trehalose overcomes the prior art
Deficiency chooses the high yeast strain of content of trehalose, and culture increases the content of trehalose in yeast cells under certain condition, uses
The yeast cultivated in this way improves production efficiency to produce trehalose, reduces production cost.
To solve the above problems, the technical solution used in the present invention is as follows：
It is a kind of for producing the rhodozyma culture method of trehalose, include the following steps：
Step 1, strain preparation, aseptically, takes inclined-plane culture, filters out that several yeast activities are high, more than viable count
Bacterial strain；
Step 2, bacterial strain screening individually cultivate the several yeast strains filtered out, after normally cultivating 24 hours, respectively
It takes 100g culture solution to carry out trehalose concentration test, chooses the highest bacterial strain of trehalose concentration；
Step 3, culture medium preparation, the culture medium parts by weight of Fiber differentiation are：Glucose 20-30g, yeast extract 2-4g, sulfuric acid
Magnesium 2g, zinc chloride 1-1.5g, sodium chloride 1.5-1.8g, molybdic acid receive 1g, vitamin 1ml, and the culture medium parts by weight normally cultivated are：
Glucose 20-30g, yeast extract 2-4g, peptone 3g, trace element solution 1ml；
Step 4, Yeast Cultivation take the bacterial strain of equivalent filtered out to be inoculated into the culture medium for filling Fiber differentiation and normal respectively
In the conical flask of the culture medium of culture, the bacterial strain of shaking flask culture Fiber differentiation 30 hours under conditions of 40-45 DEG C, at 30 DEG C
Under the conditions of normally cultivate bacterial strain 30 hours of shaking flask culture；
Step 5, content of trehalose measurement, take respectively 100g normally cultivate with the bacterial strain of Fiber differentiation, in bacterial strain yeast cells
Content of trehalose is measured.
Further, trehalose concentration test is washing, drying after centrifuge separation in the step 2, using phosphoric acid-anthrone
The concentration of method measurement trehalose.
Further, the revolving speed of shaking flask culture is 180-220r/min in the step 4.
Further, content of trehalose accounts for the 10% of yeast dry weight in the yeast cells normally cultivated in the step 4, induction
Content of trehalose accounts for 20% of yeast dry weight or more in the yeast cells of culture.
Compared with prior art, implementation result of the invention is as follows by the present invention：
It is of the present invention a kind of for producing the rhodozyma culture method of trehalose, the high yeast strain of content of trehalose is chosen,
Culture increases the content of trehalose in yeast cells under certain condition, and trehalose raising is produced with the yeast cultivated in this way
Production efficiency, reduces production cost, increases the profit of enterprise.
Specific embodiment
The present invention will be further described with reference to the examples below, but the present invention is not limited to these instances, and is being de-
Under the premise of from present inventive concept, carried out by it is any improvement be within the scope of the present invention.
Embodiment 1
It is of the present invention a kind of for producing the rhodozyma culture method of trehalose, include the following steps：
Step 1, strain preparation, aseptically, takes inclined-plane culture, filters out that several yeast activities are high, more than viable count
Bacterial strain；
Step 2, bacterial strain screening individually cultivate the several yeast strains filtered out, after normally cultivating 24 hours, respectively
It takes 100g culture solution to carry out trehalose concentration test, chooses the highest bacterial strain of trehalose concentration；
Step 3, culture medium preparation, the culture medium parts by weight normally cultivated are：Glucose 20-30g, yeast extract 2-4g, albumen
Peptone 3g, trace element solution 1ml；
Step 4, Yeast Cultivation take suitable strain inoculated filtered out into the conical flask for the culture medium normally cultivated, 30
Shaking flask culture is normally cultivated under conditions of DEG C bacterial strain 30 hours；
Step 5, content of trehalose measurement, the bacterial strain for taking 100g normally to cultivate carry out bacterial strain yeast cells intracellular trehalose content
Measurement.
Further, trehalose concentration test is washing, drying after centrifuge separation in the step 2, using phosphoric acid-anthrone
The concentration of method measurement trehalose.
Further, the revolving speed of shaking flask culture is 180-220r/min in the step 4.
Further, content of trehalose accounts for the 10% of yeast dry weight in the yeast cells normally cultivated in the step 4.
Embodiment 2
It is of the present invention a kind of for producing the rhodozyma culture method of trehalose, include the following steps：
Step 1, strain preparation, aseptically, takes inclined-plane culture, filters out that several yeast activities are high, more than viable count
Bacterial strain；
Step 2, bacterial strain screening individually cultivate the several yeast strains filtered out, after normally cultivating 24 hours, respectively
It takes 100g culture solution to carry out trehalose concentration test, chooses the highest bacterial strain of trehalose concentration；
Step 3, culture medium preparation, the culture medium parts by weight of Fiber differentiation are：Glucose 20-30g, yeast extract 2-4g, sulfuric acid
Magnesium 2g, zinc chloride 1-1.5g, sodium chloride 1.5-1.8g, molybdic acid receive 1g, vitamin 1ml；
Step 4, Yeast Cultivation take suitable strain inoculated filtered out into the conical flask for the culture medium for filling Fiber differentiation,
The bacterial strain of shaking flask culture Fiber differentiation 30 hours under conditions of 40-45 DEG C；
Step 5, content of trehalose measurement, takes the bacterial strain of 100g Fiber differentiation, carries out to bacterial strain yeast cells intracellular trehalose content
Measurement.
Further, trehalose concentration test is washing, drying after centrifuge separation in the step 2, using phosphoric acid-anthrone
The concentration of method measurement trehalose.
Further, the revolving speed of shaking flask culture is 180-220r/min in the step 4.
Further, content of trehalose accounts for 20% of yeast dry weight or more in the yeast cells of Fiber differentiation in the step 4.
Above content is only to present inventive concept example and explanation, affiliated those skilled in the art couple
Described specific embodiment does various modifications or additions or is substituted in a similar manner, without departing from invention
Conceive or beyond the scope defined by this claim, is within the scope of protection of the invention.
Claims (4)
1. a kind of for producing the rhodozyma culture method of trehalose, it is characterised in that：Include the following steps：
Step 1, strain preparation, aseptically, takes inclined-plane culture, filters out that several yeast activities are high, more than viable count
Bacterial strain；
Step 2, bacterial strain screening individually cultivate the several yeast strains filtered out, after normally cultivating 24 hours, respectively
It takes 100g culture solution to carry out trehalose concentration test, chooses the highest bacterial strain of trehalose concentration；
Step 3, culture medium preparation, the culture medium parts by weight of Fiber differentiation are：Glucose 20-30g, yeast extract 2-4g, sulfuric acid
Magnesium 2g, zinc chloride 1-1.5g, sodium chloride 1.5-1.8g, molybdic acid receive 1g, vitamin 1ml, and the culture medium parts by weight normally cultivated are：
Glucose 20-30g, yeast extract 2-4g, peptone 3g, trace element solution 1ml；
Step 4, Yeast Cultivation take the bacterial strain of equivalent filtered out to be inoculated into the culture medium for filling Fiber differentiation and just respectively
In the conical flask for the culture medium often cultivated, the bacterial strain of shaking flask culture Fiber differentiation 30 hours under conditions of 40-45 DEG C, at 30 DEG C
Under conditions of normally cultivate bacterial strain 30 hours of shaking flask culture；
Step 5, content of trehalose measurement, take respectively 100g normally cultivate with the bacterial strain of Fiber differentiation, in bacterial strain yeast cells
Content of trehalose is measured.
2. according to claim 1 a kind of for producing the rhodozyma culture method of trehalose, it is characterised in that：The step
Trehalose concentration test is washing, drying after centrifuge separation in two, using phosphoric acid-anthrone method measurement trehalose concentration.
3. according to claim 1 a kind of for producing the rhodozyma culture method of trehalose, it is characterised in that：The step
The revolving speed of shaking flask culture is 180-220r/min in four.
4. according to claim 1 a kind of for producing the rhodozyma culture method of trehalose, it is characterised in that：The step
Content of trehalose accounts for the 10% of yeast dry weight, trehalose in the yeast cells of Fiber differentiation in the yeast cells normally cultivated in four
Content accounts for 20% of yeast dry weight or more.
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| CN201810774256.6A CN108841897A (en) | 2018-07-16 | 2018-07-16 | It is a kind of for producing the rhodozyma culture method of trehalose |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302864A (en) * | 2001-02-09 | 2001-07-11 | 中国科学院微生物研究所 | High-content mycose saccharomycetes and its preparing process |
| US20050260305A1 (en) * | 2004-05-24 | 2005-11-24 | Stephen Adele | Food supplement with separate flavoring system |
| CN101824444A (en) * | 2010-04-09 | 2010-09-08 | 上海化工研究院 | Method for preparing <13>C-labelled glucose |
| CN105886573A (en) * | 2016-05-16 | 2016-08-24 | 齐鲁工业大学 | Method for preparing trehalose by continuous exoenzyme biological process |
| CN107920548A (en) * | 2015-08-06 | 2018-04-17 | 嘉吉公司 | For producing the fermentation process of steviol glycoside |
-
2018
- 2018-07-16 CN CN201810774256.6A patent/CN108841897A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302864A (en) * | 2001-02-09 | 2001-07-11 | 中国科学院微生物研究所 | High-content mycose saccharomycetes and its preparing process |
| US20050260305A1 (en) * | 2004-05-24 | 2005-11-24 | Stephen Adele | Food supplement with separate flavoring system |
| CN101824444A (en) * | 2010-04-09 | 2010-09-08 | 上海化工研究院 | Method for preparing <13>C-labelled glucose |
| CN107920548A (en) * | 2015-08-06 | 2018-04-17 | 嘉吉公司 | For producing the fermentation process of steviol glycoside |
| CN105886573A (en) * | 2016-05-16 | 2016-08-24 | 齐鲁工业大学 | Method for preparing trehalose by continuous exoenzyme biological process |
Non-Patent Citations (5)
| Title |
|---|
| YUNJIAN DU等: "Optimization of fermentation conditions for trehalose production by a marine yeast", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 * |
| 张丽杰等: "酿酒酵母培养基中主要因素对海藻糖积累的影响", 《生物技术》 * |
| 章银良等: "产海藻糖酿酒酵母培养基优化及生理学研究", 《生物技术》 * |
| 董永胜著: "《谷胱甘肽生产技术》", 31 August 2015 * |
| 赵玉巧: "产海藻糖酵母的培养条件优化研究", 《淮海工学院学报》 * |
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