CN105695345A - Mulberry powder extract and application thereof - Google Patents
Mulberry powder extract and application thereof Download PDFInfo
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- CN105695345A CN105695345A CN201610237497.8A CN201610237497A CN105695345A CN 105695345 A CN105695345 A CN 105695345A CN 201610237497 A CN201610237497 A CN 201610237497A CN 105695345 A CN105695345 A CN 105695345A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses mulberry powder extract and application thereof. A preparation method includes: adding mulberry powder which is smashed into 60-80 meshes into water; steaming at 100 DEG C for 0.5-2 h; filtering to obtain supernate which is the mulberry powder extract needed. The mulberry powder extract can be used for preparing seed culture media and fermentation culture media of male agaric mycelia, and the seed culture media and the fermentation culture media of the male agaric mycelia can be used for culture of Phellinus strains and mycelia, so that the problem of slow and unfavorable growing in the process of Phellinus liquid production fermentation is solved and yield of liquid culture mycelia is increased substantially.
Description
Technical field
The present invention relates to biological extraction and field of microbial fermentation, more particularly to a kind of mulberry powder extracting solution and application thereof。
Background technology
Owing to wild Phellinus igniarius (L. ex Fr.) Quel. is gathered by large area so that Phellinus igniarius (L. ex Fr.) Quel. resource is seriously deficient。Meanwhile, the technology of artificial culture Phellinus igniarius (L. ex Fr.) Quel., be also not as ripe, Phellinus igniarius (L. ex Fr.) Quel. condition of culture is comparatively harsh in addition, and growth cycle is long, and this makes Phellinus igniarius (L. ex Fr.) Quel. source instability。For this; adopting liquid fermentation to produce phellinus igniarius mycelium replaces Phellinus igniarius (L. ex Fr.) Quel. sporophore to become the task of top priority; the mycelium of liquid fermentation and culture has the advantages such as yield height, cultivation cycle is short, controllability is good, it is easier to industrialization continues to produce in a large number, has market prospect widely。
So far, the liquid culture phellinus igniarius mycelium under suitable condition needs the time of more than ten days, and its mycelial growth is bad and mycelium production is less。Meanwhile, current phellinus igniarius mycelium submerged fermentation culture medium is only rely on carbon nitrogen source to screen optimal medium formula, is badly in need of exploitation growth cycle short, the culture medium that yield is high。
Summary of the invention
An object of the present invention is in that to provide a kind of mulberry powder extracting solution, and it is prepared by what method prepared:
To be crushed to the weight ratio adding water, mulberry powder and water in the mulberry powder of 60-80 order is 1-3:100, filters, obtain supernatant and be required mulberry powder extracting solution after 100 DEG C of steaming and decocting 0.5-2h。
Present invention also offers a kind of seed culture medium applying phellinus igniarius mycelium prepared by above-mentioned mulberry powder extracting solution; the seed culture medium of this phellinus igniarius mycelium; being made up of mulberry powder extracting solution, potato dextrose broth and water, wherein the percentage by weight of potato dextrose broth is 2.4%；The weight ratio wherein preparing the mulberry powder used by mulberry powder extracting solution and potato dextrose broth is 1-3:2.4。
Present invention also offers the fermentation medium of a kind of phellinus igniarius mycelium prepared by above-mentioned mulberry powder extracting solution; it is characterized in that it is made up of mulberry powder extracting solution, yeast powder, glucose, potassium dihydrogen phosphate, magnesium sulfate and water, wherein the percentage by weight respectively 1%, 2%, 0.1% and 0.1% of yeast powder, glucose, potassium dihydrogen phosphate, magnesium sulfate；Wherein the mulberry powder used by mulberry powder extracting solution is 1-3:1 with the weight ratio of yeast powder。
Present invention also offers and a kind of apply the method that above-mentioned seed culture medium carries out Phellinus igniarius (L. ex Fr.) Quel. spawn culture, it is characterised in that comprise the steps:
Being inoculated in seed culture medium by the Phellinus igniarius (L. ex Fr.) Quel. strain after activation, in 24-30 DEG C, 120-180rpm incubator is cultivated 5-10 days, obtains seed liquor。
Present invention also offers and a kind of apply the method that above-mentioned fermentation medium carries out phellinus igniarius mycelium cultivation, it is characterised in that comprise the steps:
Being inoculated in fermentation medium by seed liquor by the inoculum concentration of 10%, in 24-30 DEG C, 120-180rpm incubator is cultivated 5-10 days, obtains fermentation liquid。
The present invention breaches edible fungi and cultivates the traditional view in field; seed culture medium and fermentation medium add the extracting solution of mulberry powder; both have selected the character that Phellinus igniarius (L. ex Fr.) Quel. bacterial strain relies on the relationship growth of mulberry; solve again in Phellinus igniarius (L. ex Fr.) Quel. liquid production sweat and grow comparatively slow and bad problem; the yield of Mycelia body is greatly improved simultaneously; make Phellinus igniarius (L. ex Fr.) Quel. spawn culture after 7 days seed liquor Biomass reach 7.73g/L; after Phellinus igniarius (L. ex Fr.) Quel. fermentation medium is cultivated 6 days, shaking flask Biomass reaches 17.15g/L, is respectively higher than matched group 4.43g/L and 2.42g/L。
The present invention adds mulberry powder extracting solution first in Phellinus igniarius (L. ex Fr.) Quel. fluid medium, and after cultivation, mycelial Biomass is greatly improved, and fully confirms that mulberry powder extracting solution can be greatly facilitated the submerged fermentation of thalline so that mycelial Biomass increases。
Detailed description of the invention
Strain: Phellinus igniarius (L. ex Fr.) Quel. Sanghuangporussanghuang (at fungus journal, 2016,35 (5): 1-12 deliver this bacterial strain), by Edible Fungus Inst., Shanghai Academy of Agriculture's process technology and the preservation of fermentation engineering research department
Mulberry powder (purchased from horsepower pharmaceutcal corporation, Ltd of Hui nationality)
Potato dextrose broth (purchased from American BD company)
The mensuration of mycelium dry weight: respectively by seed liquor and fermentation liquid, in the centrifugal 10min of 5000r/min, abandons supernatant, and with distilled water wash centrifugation 3-4 time, collection is deposited in 60 DEG C and is dried to constant weight, then measures mycelium dry weight。
Embodiment 1
(1) preparation of mulberry powder extracting solution: weighing pulverizing respectively for 60-80 order mulberry powder 1g, 2g, 3g, add distilled water, 100 DEG C maintain steaming and decocting 1h, filter gained supernatant and are mulberry powder extracting solution。
(2) prepared by seed culture medium: by above-mentioned three groups of extracting solution, is separately added into potato dextrose broth 2.4g, adds distilled water constant volume to 100mL, respectively as test group 1-3 group。
(3) matched group: potato dextrose broth 2.4g, adds distilled water constant volume to 100mL。
(4) four groups of culture medium carry out subpackage, in autoclave 121 DEG C, sterilizing 35min。
(5) seed culture medium: (every piece of area is about 0.3-0.5cm by Phellinus igniarius (L. ex Fr.) Quel. inoculation 6-8 block good for slat chain conveyor2) in above-mentioned four groups of culture medium, in temperature 25-26 DEG C, rotating speed is cultivate after 7 days under 150rpm, filters, it is thus achieved that mycelium measure dry weight after drying。
(6) result of the test is as shown in table 1
Table 1 seed culture mycelial biomass
Embodiment 2
(1) preparation of mulberry powder extracting solution: weighing pulverizing respectively for 60-80 order mulberry powder 1g, 2g, 3g, add distilled water, 100 DEG C maintain steaming and decocting 1h, filter gained supernatant and are mulberry powder extracting solution。
(2) prepared by fermentation medium: by above-mentioned three groups of extracting solution, is separately added into yeast autolysis powder 1g, glucose 2g, magnesium sulfate 0.1g, potassium dihydrogen phosphate 0.1g, adds distilled water constant volume to 100mL, respectively as test group 1-3 group。
(3) matched group: yeast autolysis powder 1g, glucose 2g, magnesium sulfate 0.1g, potassium dihydrogen phosphate 0.1g, adds distilled water constant volume to 100mL。
(4) four groups of culture medium carry out subpackage, in autoclave 121 DEG C, sterilizing 35min。
(5) fermentation medium: seed liquor is forwarded in culture medium with 10% inoculum concentration, in temperature 25-26 DEG C, rotating speed is cultivate after 6 days under 150rpm, filters, it is thus achieved that mycelium measure dry weight after drying。
(6) result of the test is as shown in table 2
Table 2 fermentation culture mycelial biomass
Claims (5)
1. a mulberry powder extracting solution, it is characterised in that it is prepared by what method prepared:
To be crushed to the weight ratio adding water, mulberry powder and water in the mulberry powder of 60-80 order is 1-3:100, filters, obtain supernatant and be required mulberry powder extracting solution after 100 DEG C of steaming and decocting 0.5-2h。
2. the seed culture medium of phellinus igniarius mycelium prepared by the mulberry powder extracting solution described in claim 1; it is characterized in that the seed culture medium of this phellinus igniarius mycelium; being made up of mulberry powder extracting solution, potato dextrose broth and water, wherein the percentage by weight of potato dextrose broth is 2.4%；The weight ratio wherein preparing the mulberry powder used by mulberry powder extracting solution and potato dextrose broth is 1-3:2.4。
3. the fermentation medium of phellinus igniarius mycelium prepared by the mulberry powder extracting solution described in claim 1; it is characterized in that it is made up of mulberry powder extracting solution, yeast powder, glucose, potassium dihydrogen phosphate, magnesium sulfate and water, wherein the percentage by weight respectively 1%, 2%, 0.1% and 0.1% of yeast powder, glucose, potassium dihydrogen phosphate, magnesium sulfate；The weight ratio wherein preparing the mulberry powder used by mulberry powder extracting solution and yeast powder is 1-3:1。
4. the method that the seed culture medium applied described in claim 2 carries out spawn culture, it is characterised in that comprise the steps:
Being inoculated in seed culture medium by the Phellinus igniarius (L. ex Fr.) Quel. strain after activation, in 24-30 DEG C, 120-180rpm incubator is cultivated 5-10 days, obtains seed liquor。
5. apply the method that fermentation medium described in claim 3 carries out phellinus igniarius mycelium cultivation for one kind, it is characterised in that comprise the steps:
Being inoculated in fermentation medium by seed liquor by the inoculum concentration of 10%, in 24-30 DEG C, 120-180rpm incubator is cultivated 5-10 days, obtains fermentation liquid。
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| CN201610237497.8A CN105695345A (en) | 2016-04-15 | 2016-04-15 | Mulberry powder extract and application thereof |
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| CN201610237497.8A CN105695345A (en) | 2016-04-15 | 2016-04-15 | Mulberry powder extract and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410791A (en) * | 2018-04-18 | 2018-08-17 | 浙江佐力药业股份有限公司 | Application of the extract of mulberry twig in delay Phellinus fermentation mycelium aging |
| CN110337986A (en) * | 2019-07-10 | 2019-10-18 | 杭州丝绸之路文化艺术有限公司 | A kind of Spawn incubation method of Phellinus |
| CN113995052A (en) * | 2021-07-12 | 2022-02-01 | 广西壮族自治区水牛研究所 | Grassiness sugarcane tail leaf silage and preparation method thereof |
| CN114287342A (en) * | 2021-12-22 | 2022-04-08 | 华南农业大学 | Method suitable for inducing young shoots and leaf calluses of various mulberry varieties |
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| CN102334418A (en) * | 2011-04-15 | 2012-02-01 | 付红敏 | New phellinus linteus strain and acclimation thereof |
| CN103130550A (en) * | 2013-03-20 | 2013-06-05 | 上海市农业科学院 | Culture medium and culture method of male agaric mycelium |
| CN103820333A (en) * | 2014-03-10 | 2014-05-28 | 陕西省微生物研究所 | Liquid fermentation method for improving yield of phellinus fermentation mycelia |
| CN105441333A (en) * | 2015-12-11 | 2016-03-30 | 朱吉权 | Medium and culture method for Phellinus linteus, and application of Phellinus linteus to Phellinus linteus mycelium-chrysanthemum tea |
-
2016
- 2016-04-15 CN CN201610237497.8A patent/CN105695345A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102334418A (en) * | 2011-04-15 | 2012-02-01 | 付红敏 | New phellinus linteus strain and acclimation thereof |
| CN103130550A (en) * | 2013-03-20 | 2013-06-05 | 上海市农业科学院 | Culture medium and culture method of male agaric mycelium |
| CN103820333A (en) * | 2014-03-10 | 2014-05-28 | 陕西省微生物研究所 | Liquid fermentation method for improving yield of phellinus fermentation mycelia |
| CN105441333A (en) * | 2015-12-11 | 2016-03-30 | 朱吉权 | Medium and culture method for Phellinus linteus, and application of Phellinus linteus to Phellinus linteus mycelium-chrysanthemum tea |
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| 姜福春 等: "桑树水提物对桑黄液态深层发酵的影响", 《上海农业学报》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410791A (en) * | 2018-04-18 | 2018-08-17 | 浙江佐力药业股份有限公司 | Application of the extract of mulberry twig in delay Phellinus fermentation mycelium aging |
| CN110337986A (en) * | 2019-07-10 | 2019-10-18 | 杭州丝绸之路文化艺术有限公司 | A kind of Spawn incubation method of Phellinus |
| CN113995052A (en) * | 2021-07-12 | 2022-02-01 | 广西壮族自治区水牛研究所 | Grassiness sugarcane tail leaf silage and preparation method thereof |
| CN113995052B (en) * | 2021-07-12 | 2023-11-17 | 广西壮族自治区水牛研究所 | Ivory sugarcane tail leaf silage and preparation method thereof |
| CN114287342A (en) * | 2021-12-22 | 2022-04-08 | 华南农业大学 | Method suitable for inducing young shoots and leaf calluses of various mulberry varieties |
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Application publication date: 20160622 |