CN104996166A - Cultivation method for liquid strains - Google Patents
Cultivation method for liquid strains Download PDFInfo
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- CN104996166A CN104996166A CN201510377562.2A CN201510377562A CN104996166A CN 104996166 A CN104996166 A CN 104996166A CN 201510377562 A CN201510377562 A CN 201510377562A CN 104996166 A CN104996166 A CN 104996166A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention provides a cultivation method for liquid strains. The cultivation method comprises the preparation of a culture medium and the inoculated culture of the strains, wherein the culture medium mainly comprises potato, yeast powder, agar powder, magnesium sulfate, dipotassium phosphate, peptone, white granulated sugar and the like. The cultivation method comprises cultivation of stock culture in a test tube, cultivation of stock culture in a triangular cup and cultivation of stock culture in a liquid tank, wherein the liquid strains are inoculated in a sterilized fungi bag and are cultivated for 35-45 days for fruiting. The cultivation method greatly shortens the seed production time of the liquid strains, so that the cultivation time of mushrooms is effectively shortened and is shortened by 20 days compared with an ordinary culture period, the production cost is reduced, and the contamination rate is reduced.
Description
Technical field
The present invention relates to liquid bacteria technical field, particularly relate to the cultural method of a kind of liquid spawn.
Background technology
Liquid spawn is that bacterial classification is grown by the nutrient of absorbing fluid medium with liquid nutrient medium.Liquid spawn is relative to tradition, complicated solid seed production way, the environment such as liquid spawn not only nutrition, temperature, oxygen, acid-base value are by controlling, meet the growth demand of thalline to greatest extent, and the metabolism waste gas that respiration produces can be got rid of in time, metabolism is vigorous, all mycelia cell ages are basically identical, and mycelia vigor is stronger than solid spawn.The advantages such as strain cultivation has that mobility is fast, good dispersion, germination physiology are fast, mycelial growth is fast, covering charge level morning.19 twenties in the end of the century, along with microbe application is in industrial production, microorganism is also progressively applied in edible mushroom field; In China, early than nineteen sixty mushroom submerged fermentation research by Shanghai plant physiology research institute Jin Xi, since the eighties in last century, the report about the various fermentation equipment developments of Edible Fungi is increasing.Traditional seed production way incubation time is long, and bacterial classification sends out the inconsistent situation of bacterium speed, cell age, and the infected probability of bacterial classification is high.
Summary of the invention
For above-mentioned deficiency, for solving the problems of the technologies described above, the technical solution used in the present invention is:
A cultural method for liquid spawn, described cultural method comprises the steps:
(1) transferred in inoculating hood by original seed in the test tube containing medium, the test tube of inoculation cultivates 7-9 days in bacteria case, and the bacterial classification in test tube is ripe;
(2) be forwarded in triangle cup by the mature strains in test tube through inoculating hood, be placed on shaking table by triangle cup and shake 5-10 days, the bacterial classification in triangle cup is ripe.
(3) mature strains in triangle cup inoculated under flame, in the liquid tank of bacterium of having gone out, cultivate 5-7 days, in liquid tank, bacterial classification is ripe.
(4) by cultured liquid-spawn inoculation in the bacterium bag of bacterium of having gone out, cultivate 35-45 days fruitings.
A cultural method for liquid spawn, the compound method of medium is as follows:
(1) preparation of test-tube culture medium is in weight fraction, comprise following component: water 1-3 part, potato 1-4 part, dusty yeast 0.01-0.05 part, agar powder 0.1-0.5 part, magnesium sulfate 0-0.03 part, potassium dihydrogen phosphate 0-0.04 part, peptone 0.01-0.1 part, white granulated sugar 0-0.6 part, above component is stirred, put into boiled potato water stirring to boil, inhale 0.05-0.3 part and put into test tube;
(2) preparation of triangle cup medium is in weight fraction, comprise following component: water 1-3 part, potato 1-4 part, dusty yeast 0.01-0.05 part, magnesium sulfate 0-0.03 part, potassium dihydrogen phosphate 0-0.04 part, peptone 0.01-0.1 part, white granulated sugar 0-0.6 part, above component is stirred, putting into boiled potato water stirring boils thick (too fuzzy, the concrete time of boiling), inhale 0.05-0.3 part and put into test tube;
(3) preparation of liquid tank medium is in weight fraction, comprises following component: liquid tank adds 4000 parts, water, potato starch 10-60 part, white granulated sugar 30-60 part, brown sugar 5-25 part, glucose 10-50 part, defoamer 0.7-2.0 part.
Further, the temperature of described medium is 23 DEG C-27 DEG C.
Further, the preparation of test-tube culture medium, in weight fraction, comprise following component: 1 part, water, 2 parts, potato, dusty yeast 0.03 part, agar powder 0.2 part, 0.01 part, magnesium sulfate, potassium dihydrogen phosphate 0.02 part, peptone 0.03 part, white granulated sugar 0.2 part, above component is stirred, puts into boiled potato water stirring and boil, inhale 0.05-0.3 part and put into test tube.
Further, the preparation of triangle cup medium, in weight fraction, comprise following component: 1 part, water, 2 parts, potato, dusty yeast 0.03 part, 0.01 part, magnesium sulfate, potassium dihydrogen phosphate 0.02 part, peptone 0.03 part, white granulated sugar 0.2 part, above component is stirred, puts into boiled potato water stirring and boil, inhale 0.05-0.3 part and put into test tube.
Further, the preparation of liquid tank medium, in weight fraction, comprises following component: liquid tank adds 4000 parts, water, potato starch 40 parts, white granulated sugar 50 parts, 15 parts, brown sugar, glucose 30 parts, defoamer 1 part.
The bacterial classification that the cultural method of described a kind of liquid spawn is cultivated is applied to the cultivation of flat mushroom, mushroom, Ji mushroom, elegant precious mushroom and Xingbao mushroom.
The advantage that the present invention has and good effect are: the production of hybrid seeds time substantially reducing liquid spawn, thus effectively shorten the incubation time of mushroom, shorten 20 days, reduce production cost, reduce pollution rate than common cultivation cycle.
Embodiment
The every 100g of embodiment 1 is 1 part
A cultural method for liquid spawn, described cultural method comprises the steps:
(1) preparation of test-tube culture medium, comprise following component: take water 100g, potato 200g, dusty yeast 3g, agar powder 20g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, peptone 3g, white granulated sugar 20g, above component is stirred, put into boiled potato water stirring to boil, inhale 10g and put into test tube.
(2) transferred in inoculating hood by original seed in the test tube containing medium, the test tube of inoculation cultivates 7-9 days in bacteria case, and the bacterial classification in test tube is ripe;
(3) preparation of triangle cup medium, comprise following component: take water 100g, potato 200g, dusty yeast 3g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, peptone 3g, white granulated sugar 20g, stir above component, put into boiled potato water stirring to boil, inhale 10g and put into test tube.
(4) be forwarded in triangle cup by the mature strains in test tube through inoculating hood, be placed on shaking table by triangle cup and shake 5-10 days, the bacterial classification in triangle cup is ripe.
(5) preparation of liquid tank medium, comprises following component: liquid tank adds water 400kg, potato starch 4kg, white granulated sugar 5kg, brown sugar 1.5kg, glucose 3kg, defoamer 100g.
(6) mature strains in triangle cup inoculated under flame, in the liquid tank of bacterium of having gone out, cultivate 5-7 days, in liquid tank, bacterial classification is ripe.
(7) by cultured liquid-spawn inoculation in the bacterium bag of bacterium of having gone out, cultivate 35 days fruitings.
The every 100g of embodiment 2 is 1 part
A cultural method for liquid spawn, described cultural method comprises the steps:
(1) preparation of test-tube culture medium, comprise following component: take water 150g, potato 300g, dusty yeast 5g, agar powder 10g, magnesium sulfate 3g, potassium dihydrogen phosphate 4g, peptone 1g, white granulated sugar 40g, above component is stirred, put into boiled potato water stirring to boil, inhale 10g and put into test tube.
(2) transferred in inoculating hood by original seed in the test tube containing medium, the test tube of inoculation cultivates 7-9 days in bacteria case, and the bacterial classification in test tube is ripe;
(3) preparation of triangle cup medium, comprise following component: take water 150g, potato 300g, dusty yeast 5g, magnesium sulfate 3g, potassium dihydrogen phosphate 4g, peptone 1g, white granulated sugar 40g, stir above component, put into boiled potato water stirring to boil, inhale 10g and put into test tube.
(4) be forwarded in triangle cup by the mature strains in test tube through inoculating hood, be placed on shaking table by triangle cup and shake 5-10 days, the bacterial classification in triangle cup is ripe.
(5) preparation of liquid tank medium, comprises following component: liquid tank adds water 400kg, potato starch 6kg, white granulated sugar 6kg, brown sugar 2kg, glucose 3kg, defoamer 150g.
(6) mature strains in triangle cup inoculated under flame, in the liquid tank of bacterium of having gone out, cultivate 5-7 days, in liquid tank, bacterial classification is ripe.
(7) by cultured liquid-spawn inoculation in the bacterium bag of bacterium of having gone out, cultivate 38 days fruitings.
Embodiment of the present invention is only used to further illustrate the present invention; can not be interpreted as limiting the scope of the invention, some nonessential improvement that person skilled in art makes the present invention according to content of the present invention and adjustment still belong to protection scope of the present invention.
Claims (7)
1. a cultural method for liquid spawn, is characterized in that: described cultural method comprises the steps:
(1) transferred in inoculating hood by original seed in the test tube containing medium, the test tube of inoculation cultivates 7-9 days in bacteria case, and the bacterial classification in test tube is ripe;
(2) be forwarded in triangle cup by the mature strains in test tube through inoculating hood, be placed on shaking table by triangle cup and shake 5-10 days, the bacterial classification in triangle cup is ripe.
(3) mature strains in triangle cup inoculated under flame, in the liquid tank of bacterium of having gone out, cultivate 5-7 days, in liquid tank, bacterial classification is ripe.
(4) by cultured liquid-spawn inoculation in the bacterium bag of bacterium of having gone out, cultivate 35-45 days fruitings.
2. a cultural method for liquid spawn, is characterized in that: the compound method of medium is as follows:
(1) preparation of test-tube culture medium is in weight fraction, comprise following component: water 1-3 part, potato 1-4 part, dusty yeast 0.01-0.05 part, agar powder 0.1-0.5 part, magnesium sulfate 0-0.03 part, potassium dihydrogen phosphate 0-0.04 part, peptone 0.01-0.1 part, white granulated sugar 0-0.6 part, above component is stirred, put into boiled potato water stirring to boil, inhale 0.05-0.3 part and put into test tube;
(2) preparation of triangle cup medium is in weight fraction, comprise following component: water 1-3 part, potato 1-4 part, dusty yeast 0.01-0.05 part, magnesium sulfate 0-0.03 part, potassium dihydrogen phosphate 0-0.04 part, peptone 0.01-0.1 part, white granulated sugar 0-0.6 part, above component is stirred, put into boiled potato water stirring to boil, inhale 0.05-0.3 part and put into test tube;
(3) preparation of liquid tank medium is in weight fraction, comprises following component: liquid tank adds 4000 parts, water, potato starch 10-60 part, white granulated sugar 30-60 part, brown sugar 5-25 part, glucose 10-50 part, defoamer 0.7-2.0 part.
3. the cultural method of a kind of liquid spawn according to claim 1, is characterized in that: the temperature of described medium is 23 DEG C-27 DEG C.
4. the cultural method of a kind of liquid spawn according to claim 2, it is characterized in that: the preparation of test-tube culture medium, in weight fraction, comprise following component: 1 part, water, 2 parts, potato, dusty yeast 0.03 part, agar powder 0.2 part, 0.01 part, magnesium sulfate, potassium dihydrogen phosphate 0.02 part, peptone 0.03 part, white granulated sugar 0.2 part, above component is stirred, puts into boiled potato water stirring and boil, inhale 0.05-0.3 and put into test tube.
5. the cultural method of a kind of liquid spawn according to claim 2, it is characterized in that: the preparation of triangle cup medium, in weight fraction, comprise following component: 1 part, water, 2 parts, potato, dusty yeast 0.03 part, 0.01 part, magnesium sulfate, potassium dihydrogen phosphate 0.02 part, peptone 0.03 part, white granulated sugar 0.2 part, above component is stirred, puts into boiled potato water stirring and boil, inhale 0.05-0.3 and put into test tube.
6. the cultural method of a kind of liquid spawn according to claim 2, it is characterized in that: the preparation of liquid tank medium, in weight fraction, comprise following component: liquid tank adds 4000 parts, water, potato starch 40 parts, white granulated sugar 50 parts, 15 parts, brown sugar, glucose 30 parts, defoamer 1 part.
7. the bacterial classification that the cultural method of a kind of liquid spawn as described in claim 1-6 is cultivated is applied to the cultivation of flat mushroom, mushroom, Ji mushroom, elegant precious mushroom and Xingbao mushroom.
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| CN201510377562.2A CN104996166A (en) | 2015-07-01 | 2015-07-01 | Cultivation method for liquid strains |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340580A (en) * | 2015-12-01 | 2016-02-24 | 仇颖超 | Method for cultivating agaricus bisporus through liquid strains |
| CN106036446A (en) * | 2016-07-01 | 2016-10-26 | 河北大学 | Pleurotus pulmonarius flavored potato and jujube steamed sponge cakes and preparation method thereof |
| CN107488596A (en) * | 2017-08-16 | 2017-12-19 | 什邡昊阳农业发展有限公司 | A kind of liquid producing method for seed of agaric |
| CN109258306A (en) * | 2018-11-15 | 2019-01-25 | 江苏强农农业技术服务有限公司 | A kind of elegant precious mushroom strain mating system rapidly and efficiently |
| CN109845582A (en) * | 2019-01-07 | 2019-06-07 | 贵州正黔门大健康产业有限公司 | A kind of preparation method of Coriaria sinica mushroom liquid bacterial |
| CN110583371A (en) * | 2019-09-23 | 2019-12-20 | 贵州同辉食用菌发展有限公司 | Fermentation method of mushroom liquid strain with high activity and high stability |
| CN112088720A (en) * | 2020-09-19 | 2020-12-18 | 灌南云农食用菌研究所(有限合伙) | Pleurotus eryngii liquid culture medium and pleurotus eryngii liquid culture production process |
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| CN101829159A (en) * | 2010-06-03 | 2010-09-15 | 樊美珍 | Method for preparing selenium-enriched monkey head mushroom capsule |
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Patent Citations (6)
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| JPH02154621A (en) * | 1988-12-07 | 1990-06-14 | Komatsugawa Kakoki Kk | Inoculation of fungi seeds and device therefor |
| CN1742543A (en) * | 2005-10-29 | 2006-03-08 | 付嵘 | Commercial production method for edible mushroom liquid culture bacteria, apparatus and automatic sterilized inoculator |
| CN101829159A (en) * | 2010-06-03 | 2010-09-15 | 樊美珍 | Method for preparing selenium-enriched monkey head mushroom capsule |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340580A (en) * | 2015-12-01 | 2016-02-24 | 仇颖超 | Method for cultivating agaricus bisporus through liquid strains |
| CN106036446A (en) * | 2016-07-01 | 2016-10-26 | 河北大学 | Pleurotus pulmonarius flavored potato and jujube steamed sponge cakes and preparation method thereof |
| CN107488596A (en) * | 2017-08-16 | 2017-12-19 | 什邡昊阳农业发展有限公司 | A kind of liquid producing method for seed of agaric |
| CN109258306A (en) * | 2018-11-15 | 2019-01-25 | 江苏强农农业技术服务有限公司 | A kind of elegant precious mushroom strain mating system rapidly and efficiently |
| CN109845582A (en) * | 2019-01-07 | 2019-06-07 | 贵州正黔门大健康产业有限公司 | A kind of preparation method of Coriaria sinica mushroom liquid bacterial |
| CN110583371A (en) * | 2019-09-23 | 2019-12-20 | 贵州同辉食用菌发展有限公司 | Fermentation method of mushroom liquid strain with high activity and high stability |
| CN112088720A (en) * | 2020-09-19 | 2020-12-18 | 灌南云农食用菌研究所(有限合伙) | Pleurotus eryngii liquid culture medium and pleurotus eryngii liquid culture production process |
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