CN108837132A - A kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans - Google Patents
A kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans Download PDFInfo
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Abstract
The present invention relates to the essential oil of traditional Chinese medicine for the treatment of thromboangitis obliterans, the treatment safety of thromboangitis obliterans can effectively be solved, good effect, medication problem at low cost, by Radix Salviae Miltiorrhizae essential oil 6-8ml, Radix Glycyrrhizae essential oil 6-8ml, safflower essential oil 2-4ml, ginger essential oil 6-8ml, ramulus cinnamomi essential oil 4-6ml, trigone essential oil 6.5-8.5ml, Radix Notoginseng essential oil 4-6ml, viola mandshurica essential oil 6-7ml, angelica essential oil 6-7ml and borneol 5-7g mixing is made, compatibility of the present invention is scientific and reasonable, abundant raw material, preparation easy to produce, it is at low cost, it is easy to use, effect is good, effective for thromboangitis obliterans, it is the innovation treated on thromboangitis obliterans drug, there is significant economic and social benefit.
Description
Technical field
The present invention relates to medicine, especially a kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans（Also known as three red red essences
Oil）.
Background technique
Thromboangitis obliterans is a kind of common peripheral vessels chmnic obstructive's inflammatory disorders.Mainly involve four limbs
In, parteriole and vein, but it is the most common with lower limb, occasionally can be involved mesenterium, the cerebrovascular and coronary artery.Blood in arterial lumen
Bolt occlusion, tube wall fibrosis；Later period with capable vein and nerve adhesion at tough and tensile fiber rope item.Affected limbs Keyin nutrient
Obstacle and integumentary musculature atrophy occurs, or even gangrene, motherland's medicine are referred to as " gangrene of finger or toe ", and the body for drastically influencing people is strong
Health.Though the existing a variety of drugs for having treatment thromboangitis obliterans, for various reasons, using and curative effect and people not to the utmost
Meaning, therefore, improvement and innovation on treatment thromboangitis obliterans drug are the technical issues that need to address.
Summary of the invention
For above situation, for the defect for overcoming the prior art, the purpose of the present invention is just to provide a kind of blood vessel for the treatment of and closes
The essential oil of traditional Chinese medicine of plug property vasculitis, can effectively solve the treatment safety, good effect, medication at low cost of thromboangitis obliterans
Problem.
The technical solution that the present invention solves is a kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans, by Radix Salviae Miltiorrhizae essential oil
6-8ml, Radix Glycyrrhizae essential oil 6-8ml, safflower essential oil 2-4ml, ginger essential oil 6-8ml, ramulus cinnamomi essential oil 4-6ml, trigone essential oil 6.5-
8.5ml, Radix Notoginseng essential oil 4-6ml, viola mandshurica essential oil 6-7ml, angelica essential oil 6-7ml and borneol 5-7g mixing are made；
The Radix Salviae Miltiorrhizae essential oil is that Radix Salviae Miltiorrhizae was ground into the coarse powder of 10~20 meshes, supercritical CO2Fluid extraction, feed liquid weight
Measure volume ratio 1:10~20, w/v refers to solid in terms of g, and liquid is in terms of mL, 42~50 DEG C of extraction temperature, extracting pressure
20~25MPa, obtains Radix Salviae Miltiorrhizae essence by 50~60 DEG C of separation temperature, 4.5~5.5MPa of separating pressure, 1.5~2.5h of extraction time
Oil；
The Radix Glycyrrhizae essential oil, safflower essential oil, ginger essential oil, ramulus cinnamomi essential oil, trigone essential oil, Radix Notoginseng essential oil, viola mandshurica essential oil,
Radix Salviae Miltiorrhizae with the preparation method of Radix Salviae Miltiorrhizae essential oil, need to only be changed respectively into Radix Glycyrrhizae, safflower, ginger, osmanthus by the preparation method of angelica essential oil
Branch, trigone, Radix Notoginseng, viola mandshurica, Radix Angelicae Sinensis, are not repeated herein.
Compatibility of the present invention is scientific and reasonable, abundant raw material, preparation easy to produce, at low cost, easy to use, effect is good, effective
In thromboangitis obliterans, it is the innovation treated on thromboangitis obliterans drug, there is significant economic and social benefit.
Specific embodiment
It elaborates below in conjunction with concrete condition and embodiment to a specific embodiment of the invention.
The present invention in specific implementation, can be provided by following embodiment.
Embodiment 1
The present invention in specific implementation, a kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans, by Radix Salviae Miltiorrhizae essential oil 7ml, Radix Glycyrrhizae
It is essential oil 7ml, safflower essential oil 3ml, ginger essential oil 7ml, ramulus cinnamomi essential oil 5ml, trigone essential oil 7.5ml, Radix Notoginseng essential oil 5ml, pale reddish brown
Butyrin oil 5ml, angelica essential oil 5ml and borneol 6g mixing are made；
The Radix Salviae Miltiorrhizae essential oil is that Radix Salviae Miltiorrhizae was ground into the coarse powder of 10~20 meshes, supercritical CO2Fluid extraction, feed liquid weight
Measure volume ratio 1:10~20, w/v refers to solid in terms of g, and liquid is in terms of mL, 42~50 DEG C of extraction temperature, extracting pressure
20~25MPa, obtains Radix Salviae Miltiorrhizae essence by 50~60 DEG C of separation temperature, 4.5~5.5MPa of separating pressure, 1.5~2.5h of extraction time
Oil；
The Radix Glycyrrhizae essential oil, safflower essential oil, ginger essential oil, ramulus cinnamomi essential oil, trigone essential oil, Radix Notoginseng essential oil, viola mandshurica essential oil,
Radix Salviae Miltiorrhizae with the preparation method of Radix Salviae Miltiorrhizae essential oil, need to only be changed respectively into Radix Glycyrrhizae, safflower, ginger, osmanthus by the preparation method of angelica essential oil
Branch, trigone, Radix Notoginseng, viola mandshurica, Radix Angelicae Sinensis, are not repeated herein.
Embodiment 2
The present invention in specific implementation, a kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans, by Radix Salviae Miltiorrhizae essential oil 6.5ml, sweet
Careless essential oil 7.5ml, safflower essential oil 2.5ml, ginger essential oil 7.5ml, ramulus cinnamomi essential oil 4.5ml, trigone essential oil 8ml, Radix Notoginseng essential oil
4.5ml, viola mandshurica essential oil 6.5ml, angelica essential oil 6.5ml and borneol 6.5g mixing are made.
Embodiment 3
The present invention in specific implementation, a kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans, by Radix Salviae Miltiorrhizae essential oil 7.5ml, sweet
Careless essential oil 6.5ml, safflower essential oil 3.5ml, ginger essential oil 6.5ml, ramulus cinnamomi essential oil 5.5ml, trigone essential oil 7ml, Radix Notoginseng essential oil
5.5ml, viola mandshurica essential oil 6.5ml, angelica essential oil 6.5ml and borneol 5.5g mixing are made.
The preparation method of above-mentioned each essential oil is routine techniques in fact, and core of the invention is that component, key are to use
Amount, each component are all commercially available when necessary, and creative contribution of the invention is there is the carry out compatibility of each component science
Effectiveness achieves extraordinary advantageous effects in treatment thromboangitis obliterans, and through test, and related testing data is such as
Under：
One, animal experiment
1 experimental material
1.1 laboratory apparatus
FA (N)/JA (N) a series of electronic balances, Shanghai Min Qiao precision instrument Co., Ltd；
1.2 medicine and reagent
Essential oil of traditional Chinese medicine of the present invention, also known as three red red essential oils.
Sodium laurate, Shanghai Yuan Ye Biotechnology Co., Ltd, lot number X08S6W3134；TONGSAIMAI PIAN, Jiangsu Kang Yuan sun
Light pharmaceutcal corporation, Ltd, lot number 161218；Physiological saline, Kelun Pharm Ind Co., Ltd., Sichuan, lot number N17021703-1；
Penicillin, Huabei Pharmaceutic Co., Ltd, lot number F7038524；Thromboxane (TXB2), Suzhou Calvin biotechnology are limited
Company, lot number 20180110SR；Ketone-prostaglandin (6-K-PGF1 α), Suzhou Calvin Biotechnology Co., Ltd, lot number
20180110SR；Endothelin (ET), Suzhou Calvin Biotechnology Co., Ltd, lot number 20180110SR；Interleukin 1
(IL-1), Suzhou Calvin Biotechnology Co., Ltd, lot number 20180110SR.
1.3 experimental animal
300-320g male rat, SPF grades, certification of fitness No.37009200009495, the license of Shandong Province's Experimental Animal Center
The Shandong card SCXK 20140007.Experimental unit is numbered using licensing：SYXK（Henan）2015-0005.
2 experimental methods
2.1 animal feeding
22-26 DEG C of temperature, humidity 40-60%, artificial light replaced 12 hour daytime with 12 hour night, gave conventional experiment
Room diet, not water restriction.
2.2 experimental method
Wistar rat is randomly divided into 5 groups, respectively sham-operation group, model group, aspirin positive controls, large and small dosage
Three red red essential oil groups, every group of 10 rats.Rat adaptive feeding 5 days, fasting 12 hours before modeling.We using according to
The method of Ashida establishes rat suppository obliterans mould [1].First with 10% hydration chloric acid according to the dosage abdomen of 3mL/Kg
Chamber injection enters the anesthesia stage of stable development by rat anesthesia, to rat, and rat is faced upward position and is fixed on operating table, left lower extremity medial side is cut
Hair, Iodophor local skin disinfection, takes stringer notch on the inside of groin midpoint to knee, is about 1.5-2.0cm notch, separates subcutaneous group
It knits, dissociate femoral artery, proximal femoral artery clamp blocking blood flow.Model group distal end injection 0.2mL below artery clamp
L0mg/m L sodium laurate solution shows slightly white when without bright wet swelling, softly takes needle until blood vessel obviously fills；Sham-operation
Group is in the physiological saline of same position injection equivalent.Each group removes artery clamp after injecting 15min, and pressing haemostatic looks into no work
Dynamic property bleeding, suture muscle, subcutaneous tissue and skin.Postoperative 200,000 unit antibiotics penicillin of every rats by intraperitoneal injection, in advance
Anti- infection.The violaceous ischemic change of next day limbs appearance shows that model is successfully prepared after hand.Second day after modeling, start to
Medicine.The red red essential oil group daily morning and afternoon of large dosage three applies 0.1ml/cm2 in suffering limb, and the red red essential oil group every morning of large dosage three exists
Suffering limb applies 0.1ml/cm2, and sham-operation group and model group give isometric atoleine, and TONGSAIMAI PIAN group gavages suspension
（100mg/kg）.Daily administration, successive administration 10 days.
Since modeling, the sign variation of every group of rat operation hind leg, including skin temperature and color, limbs are observed daily
Situations such as swelling, bad trace, mummified degree and broken row.It grades to rat suffering limb：I grade；Lesion is confined to toenail portion；
II grade：Lesion is confined to toe；III grade：Lesion is confined to sufficient claw；IV grade：Lesion is confined to knee joint or less；V grade：Lesion
Develop to knee joint or more.
After the last administration, by after rat chloral hydrate anesthesia, abdominal cavity is splitted, it is complete to extract rat from abdominal aortic blood
Blood, 4000r/min are centrifuged 10min, carefully supernatant are sucked out, and dispense, and -80 DEG C of preservations are to be measured.Measure thromboxane in sample
(TXB2)；The content of 6- ketone-prostaglandin (6-K-PGF1 α), Endothelin (ET), interleukin 1 (IL-1).A free left side out
Lower limb femoral artery is immersed in formalin from the artery of one section of 2-3cm long of point of puncture lower section interception, and pathology is observed in HE dyeing
Variation.
2.3 statistical method
Experimental data with SPSS21 statistical software handle, data with (± s) it indicates, comparison among groups are examined using t.
3 experimental results
The influence of 3.1 pairs of rat general status
Before modeling, all rat hair colors are pure white glossy, and tail color is light red, and coat is closely personal, and activity is quick, and the state of mind is good
It is good.Stool is in granular form, and color is greyish black, the micro- Huang of urine.
Rat situation during modeling and administration：Rat is after femoral artery injects the modeling of sodium laurate solution, after about 10min
Start to occur that modeling side hind leg foot claw is pale, and some is then livid purple, local skin temperature reduces, and arteriopalmus weakens or disappears
It loses.Next day all animal model modeling side limbs foot claw starts pale, the swelling that occur different degrees of, and suffering limb has ischemic
Performance, livid purple color are deepened and are gradually developed upwards, occur walking lamely or traction phenomena.Serious animal model toes blackening, and by
Gradually development upwards, forms gangrene.There is modeling side hind leg gangrene partial exfoliation in partial rat model.Animal model modeling after a week
There is different degrees of infection in side hind leg, and acra erosion, ulcer and inflammatory reaction have fester exudation, foul smell, and a small number of rats suffer from
There is severe necrosis in side gangrene partial exfoliation, the tibialis posterior group of suffering limb, and the sign of general infection occurs in partial rat model, performance
For symptoms such as abdominal distension, tripping, syntexis, few foods.It can obviously improve appearance symptom with essential oil group.
The influence of 3.2 pairs of apparent indexs of rat
Blank control group rat hindlimb muscle color is fresh, ruddy, and vein, artery are unobstructed, through the visible kermesinus of wall of vein
Blood flow.
Model control group rat sample is observed that the muscle group of modeling side hind leg has different degrees of necrosis to sexually revise, bad
Most of dead type is caseous necrosis, partially with infection, it is seen that fester exudation, foul smelling, distal limb show as stemness
Gangrene, after falling off, hind leg bone is exposed, some TAO rat models, is showed only as cross mountains row and towing, hind leg shape is complete, nothing
Gangrene performance, but there are still muscular deaths for limb behind.Each group rat affected part significantly improves after external essence oil group intervention.
Observe rat suffering limb skin color, temperature, whether there is or not swelling, whether there is or not gangrene and gangrene degree, walk lamely situations such as.Disease
Become grading：I grades, lesion is confined to toenail portion；II grades, lesion is confined to toe；III grades, lesion is confined to sufficient claw；
IV grades, lesion is confined to knee joint or less；V grades, it is more than pathological development to knee joint.
The influence that the red red essential oil group of table 1 three changes Buerger's disease rat sign
| Group | Animal number of elements | Nothing | I grade | II grade | III grade | IV grade | V grade |
| Blank group | 10 | 10 | |||||
| Model group | 10 | 4 | 2 | 4 | |||
| TONGSAIMAI PIAN group | 10 | 5 | 2 | 3 | |||
| The red red essential oil group of large dosage three | 10 | 8 | 1 | 1 | |||
| The red red essential oil group of low dose three | 10 | 1 | 1 | 2 | 3 | 3 |
Note：** P<0.01, * P<0.05, it indicates and model group ratio
As shown in Table 1, blank group rat all goes well.With blank group ratio, there is significant sign lesion in model group.With model group phase
Than the red red essential oil group group of large dosage three and TONGSAIMAI PIAN group can significantly mitigate Buerger's disease rat sign lesion（P<
0.01）, the red red essential oil group group of small dose group has the tendency that mitigating Buerger's disease rat sign lesion（P>0.05）.
The influence of 3.3 pairs of Buerger's disease rat blood serum indexs
The red red essential oil of 2 large dosage of table three is to Buerger's disease rat model serum T XB2, ET, IL-1 and 6-k-PGF1 α
Horizontal influence (± s, n=10)
| Group | TXB2 | ET | IL-1 | 6-k-PGF1α |
| Blank group | 1161.88±103.51** | 141.55±13.37** | 81.7±7.02** | 1317.14±148.04** |
| Model group | 1495.63±106.87 | 191.55±11.38 | 102.77±9.4 | 952.86±102.85 |
| TONGSAIMAI PIAN group | 1240±119.66** | 155.21±12.85** | 85.47±8.3** | 1181.43±94.7** |
| The red red essential oil group of large dosage three | 1218.75±75.88** | 139.01±11.04** | 86.5±8.08** | 1259.29±96.2** |
| The red red essential oil group of low dose three | 1396.25±115.34 | 175.56±13.51* | 94.77±3.18* | 1005.71±74.11 |
Note：** P<0.01, * P<0.05, it indicates compared with model group
As shown in Table 2, compared with blank group, Buerger's disease model group rats serum T XB2, ET, IL-1 are horizontal significant
It increases（P<0.01）, 6-k-PGF1 α significant decrease（P<0.01）.Compared with model group, the red red essential oil group of large dosage three and logical plug
It is horizontal that arteries and veins piece group can significantly reduce serum T XB2, ET, IL-1（P<0.01）, can significantly increase serum 6-k-PGF1 alpha levels；Small dose
The red red essential oil group of amount three can obviously reduce ET, IL-1 level（P<0.05）.
3.4 on the histological influence of Buerger's disease rat part pathology
Each group lesion local organization morphologic observation result：Blank group blood vessel is normal, and vascular wall thickness is normal, and smooth muscle, elastic force are fine
Dimension, endothelial cell are normal, intravascular no thrombosis, no inflammatory cell infiltration.Model group Intravascular Thrombus is formed；In thrombus
Connective tissue proliferation, vascular endothelial cell deformation, necrosis or fall off, tube wall is thinning, and smooth muscle cell core is rounded.TONGSAIMAI PIAN
Group, thrombosis, thrombus range are reduced compared with model group, and vascular endothelial cell falls off, and with bleeding, blood vessel surrounding connective tissue
Hyperplasia, inflammatory cell infiltration.The red red essential oil group blood vessel of large dosage three is normal, and thrombus range is reduced compared with model group large area；
Vascular wall thickness is normal, and smooth muscle arrangement is normal, and slight endothelial cell and elastic fibers fall off, other have no that work becomes.Small dose
The red red essential oil group Intravascular Thrombus of amount three is formed, and vascular endothelial cell falls off, blood vessel surrounding connective tissue hyperplasia, inflammatory cell leaching
Profit.
4. conclusion
The red red essential oil group group of large dosage three can significantly mitigate Buerger's disease rat sign lesion.The red red essence of large dosage three
Oily group can significantly reduce serum T XB2, ET, IL-1 level, can significantly increase serum 6-k-PGF1 alpha levels；The red red essence of low dose three
Oily group can obviously reduce ET, IL-1 level.The red red essential oil group blood vessel of large dosage three is normal, and thrombus range is compared with the big face of model group
Product is reduced；Vascular wall thickness is normal, and smooth muscle arrangement is normal, and slight endothelial cell and elastic fibers fall off, other have no work
Become.The red red essential oil group Intravascular Thrombus of low dose three is formed, and vascular endothelial cell falls off, blood vessel surrounding connective tissue hyperplasia, scorching
Property cellular infiltration.The red red essential oil external application of prompt three can significantly mitigate Buerger's disease rat sign, biochemical indicator, part
The pathological change of tissue morphology.
Two, clinical test
1, the standard of case is selected
Thromboangitis obliterans is a kind of common peripheral vessels chmnic obstructive's inflammatory disorders.Mainly involve in four limbs,
Parteriole and vein, but it is the most common with lower limb, it occasionally can be involved mesenterium, the cerebrovascular and coronary artery.Thrombus in arterial lumen
Occlusion, tube wall fibrosis；Later period with capable vein and nerve adhesion at tough and tensile fiber rope item.Affected limbs Keyin nutrient barrier
Hinder and integumentary musculature atrophy occurs, or even gangrene, motherland's medicine is referred to as " gangrene of finger or toe ", drastically influences the health of people.
It is mainly shown as：
Pain：(1) intermittent claudication：After patient walks a certain distance, shank or muscle of foot occur distending pain or take out pain, are forced
It halts, after taking a momentary rest, pain is alleviated rapidly, then pain is appeared again existing again after walking, and this symptom is known as intermittent claudication.
(2) rest pain：It is prolonged that suffering limb is in resting state pain.Its pain is violent, and night, more so when limb elevation added
Weight, mitigates when sagging.
Cacesthesia：Suffering limb send out cool or it is scorching hot, itch, needle thorn, acid is swollen, numb etc. feels, even have the sense differed in size
Feel completely loses area.
Dystrophia：Suffering limb dry skin, furfur, chap, hypohidrosis or lossless, fine hair fall off, toe（Refer to）Attenuate, toe（Refer to）
First thickens or crisp fritter deformation, limb muscle relaxation, atrophy；Acra ulcer or necrosis, with affected limb swelling, color of the leather is dark red or purple dark.
Limb artery beating weakens or disappears.
Migrans superficial phlebitis：About 40% patient occurs migrans repeatedly before the onset or in the process in shank or foot
Superficial thrombophlebitis.
2, diagnostic criteria
It is formulated standard according to national Buerger's disease meeting, is made a definite diagnosis according to the classification of three phase three-levels, it is all that there is above-mentioned symptom
Person can classify according to three phase three-levels and make a definite diagnosis with thromboangitis obliterans.
3, therapeutic scheme
Essential oil of traditional Chinese medicine of the present invention is locally applied in lesion daily 2 times, embrocate repeatedly every time l-2 hours, 20 days as one therapeutic course.
Using being aided with western medicine routine treatment during essential oil as needed.
4, efficacy assessment standard
It is executed according to the criterion of therapeutical effect that national Buerger's disease meeting is formulated.
It cures：Clinical symptoms disappear, and get well.
Effectively：Clinical symptoms have improvement or are obviously improved, and need to further treat.
In vain：Clinical symptoms even have the trend of exacerbation without improvement.
5, statistical procedures
Through being treated to determining thromboangitis obliterans patient 36 using essential oil of traditional Chinese medicine of the present invention, provided according to above-mentioned
Technical solution treatment after, be statistically analyzed, wherein：Male 30, it is female 6, the age 30 1 60 years old, 41 years old average；The course of disease is most
It is 2 years long, it is most 6 months short；Lesion is single lower limb toe-end.Diagnosis and stage and step, according to national Buerger's disease
Meeting is formulated standard, and is classified according to three phase three-levels, this pathology is 1,2 phase patients, belongs to 15 of I phase, accounts for 41.7
%；21 of 2 phases, account for 58.3 %.
Journey is controlled using essential oil of traditional Chinese medicine of the present invention, cures 30 (83.33%), improve 4 (11.11%), and invalid 2
Example (5.56%), total effective rate is up to 94.44%.
6, conclusion
Experiment shows that instant component compatibility is scientific and reasonable, abundant raw material, and preparation easy to produce is at low cost, convenient to take, effect
It is good.Thromboangitis obliterans, it is early on the books in motherland's medicine,《Yizong Jinjian》,《Surgery is authentic》There is detailed record.
Such as:The gangrene of finger or toe, gangrene, vascular disorders etc..Its etiology and pathogenesis is mainly:Cold sensation of the genitalia visitor is in train of thought, cold blood stasis, blood stasis stasis, and the stasis of blood is long
Change heat, heat toxin stops up stagnant, blood losing corruption.Radix Salviae Miltiorrhizae, trigone, Radix Notoginseng, safflower, Radix Angelicae Sinensis activating microcirculation and removing stasis medicinal, can be relieved blood stasis in side of the present invention
Stasis；Ginger, ramulus cinnamomi, safflower warming meridian can be relieved cold sensation of the genitalia visitor in train of thought, cold blood stasis；Viola mandshurica, Radix Glycyrrhizae are clearing heat and detoxicating
And promoting blood circulation, it can be relieved the stasis of blood and change heat long, heat toxin stops up stagnant, blood losing corruption.To eliminate the tcm causa morbi of thromboangitis obliterans
The interpretation of the cause, onset and process of an illness enables above-mentioned all diseases to alleviate rehabilitation.And the present invention finds no toxic side effect and adverse reaction, table in experiment
Bright drug safety, curative effect is reliable and stable, and curative effect is up to 94% or more, is the innovation treated on thromboangitis obliterans drug, has
Significant economic and social benefit.
Claims (4)
1. a kind of essential oil of traditional Chinese medicine for treating thromboangitis obliterans, which is characterized in that by Radix Salviae Miltiorrhizae essential oil 6-8ml, Radix Glycyrrhizae essential oil
6-8ml, safflower essential oil 2-4ml, ginger essential oil 6-8ml, ramulus cinnamomi essential oil 4-6ml, trigone essential oil 6.5-8.5ml, Radix Notoginseng essential oil 4-
6ml, viola mandshurica essential oil 6-7ml, angelica essential oil 6-7ml and borneol 5-7g mixing are made；
The Radix Salviae Miltiorrhizae essential oil is that Radix Salviae Miltiorrhizae was ground into the coarse powder of 10~20 meshes, supercritical CO2Fluid extraction, feed liquid weight
Volume ratio 1:10~20, w/v refers to solid in terms of g, and liquid is in terms of mL, 42~50 DEG C of extraction temperature, extracting pressure 20
~25MPa, 50~60 DEG C of separation temperature, 4.5~5.5MPa of separating pressure, 1.5~2.5h of extraction time obtain Radix Salviae Miltiorrhizae essential oil；
The Radix Glycyrrhizae essential oil, safflower essential oil, ginger essential oil, ramulus cinnamomi essential oil, trigone essential oil, Radix Notoginseng essential oil, viola mandshurica essential oil,
The preparation method of angelica essential oil is the same as the preparation method of Radix Salviae Miltiorrhizae essential oil.
2. the essential oil of traditional Chinese medicine for the treatment of thromboangitis obliterans according to claim 1, which is characterized in that by Radix Salviae Miltiorrhizae essential oil
7ml, Radix Glycyrrhizae essential oil 7ml, safflower essential oil 3ml, ginger essential oil 7ml, ramulus cinnamomi essential oil 5ml, trigone essential oil 7.5ml, Radix Notoginseng essential oil
5ml, viola mandshurica essential oil 5ml, angelica essential oil 5ml and borneol 6g mixing are made.
3. the essential oil of traditional Chinese medicine for the treatment of thromboangitis obliterans according to claim 1, which is characterized in that by Radix Salviae Miltiorrhizae essential oil
6.5ml, Radix Glycyrrhizae essential oil 7.5ml, safflower essential oil 2.5ml, ginger essential oil 7.5ml, ramulus cinnamomi essential oil 4.5ml, trigone essential oil 8ml, three
Seven essential oil 4.5ml, viola mandshurica essential oil 6.5ml, angelica essential oil 6.5ml and borneol 6.5g mixing are made.
4. the essential oil of traditional Chinese medicine for the treatment of thromboangitis obliterans according to claim 1, which is characterized in that by Radix Salviae Miltiorrhizae essential oil
7.5ml, Radix Glycyrrhizae essential oil 6.5ml, safflower essential oil 3.5ml, ginger essential oil 6.5ml, ramulus cinnamomi essential oil 5.5ml, trigone essential oil 7ml, three
Seven essential oil 5.5ml, viola mandshurica essential oil 6.5ml, angelica essential oil 6.5ml and borneol 5.5g mixing are made.
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| CN110812467A (en) * | 2019-12-12 | 2020-02-21 | 河南中医药大学 | External pine and cinnamon essential oil for promoting fracture rehabilitation |
| CN110898204A (en) * | 2019-12-18 | 2020-03-24 | 河南中医药大学 | External-use Huanggui medicinal essential oil for treating postoperative abdominal distension |
| CN111643647A (en) * | 2020-04-30 | 2020-09-11 | 南京益鸣生物科技有限公司 | Traditional Chinese medicine composition for blood vessel maintenance |
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Cited By (3)
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| CN110812467A (en) * | 2019-12-12 | 2020-02-21 | 河南中医药大学 | External pine and cinnamon essential oil for promoting fracture rehabilitation |
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