CN108827478A - A kind of photon measurement system based on the wide range of linearity of two-photon counter - Google Patents
A kind of photon measurement system based on the wide range of linearity of two-photon counter Download PDFInfo
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- CN108827478A CN108827478A CN201810749438.8A CN201810749438A CN108827478A CN 108827478 A CN108827478 A CN 108827478A CN 201810749438 A CN201810749438 A CN 201810749438A CN 108827478 A CN108827478 A CN 108827478A
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- 238000005259 measurement Methods 0.000 title claims abstract description 23
- 238000012545 processing Methods 0.000 claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 239000000523 sample Substances 0.000 claims abstract description 4
- 238000009738 saturating Methods 0.000 claims 1
- 230000003287 optical effect Effects 0.000 abstract description 7
- 238000003556 assay Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 4
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- 230000005540 biological transmission Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012502 diagnostic product Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J11/00—Measuring the characteristics of individual optical pulses or of optical pulse trains
Abstract
The present invention relates to the photon measurement systems based on the wide range of linearity of two-photon counter, belong to technical field of optical detection, the light that measured object issues is incident to the spectro-film of light-dividing device through lamp guide, the transmitted light transmitted through spectro-film, which is incident to, surveys low value photon counter, it is incident to after diaphragm and attenuator through the reflected light of spectro-film reflection and surveys high level photon counter, calculation processing apparatus judges whether the first measured value for surveying the output of high level photon counter is greater than threshold value, threshold value is to survey the corresponding number of photons for surveying high level photon counter in linear highest point of low value photon counter, if, then calculation processing apparatus output photon probe value, photon detection value is the product of the splitting ratio of the first measured value and spectro-film.The present invention is combined using low value photon counter and survey high level photon counter is surveyed, the wide line range detection of faint light relative light intensity is realized, to meet the demand of wide range of linearity measurement needed for the fields such as chemiluminescence immune assay field or the detection of other light energies.
Description
Technical field
The present invention relates to technical field of optical detection, more particularly to a kind of based on the wide range of linearity of two-photon counter
Photon measurement system.
Background technique
Light is by the molecular photon stream of light, and photon is a kind of without rest mass, but has the particle of energy (momentum).Root
According to the calculation formula E=h ν of Einstein's photon energy0=hc/ λ, can calculate the power of single photon, if wavelength is 435nm
Single photon power be 4.6 × 10-19W, it is desirable to the light energy detection for carrying out single-photon-level, at present with photoelectricity times under scientific and technological level
Increase pipe as the photon counter sensitivity highest of sensor, be widely used in weak light detection field, especially in-vitro diagnosis produces
Product (in vitro diagnostic products, IVD) industry, most of chemical illumination immunity analysis instruments all use photon meter
Number device is that project is more, test philosophy is complicated, certain project linear models the characteristics of chemiluminescence immune assay as sensor
Width etc. is enclosed, therefore optical measuring device is needed to have the wider range of linearity, and the range of linearity of the quasi-instrument is generally relatively narrow at present, nothing
Method meets the wide range of linearity needed for chemiluminescence immune assay.
Summary of the invention
Based on this, it is necessary to carry out the spy of faint light relative light intensity using photon counter in current field of optical detection
Range of linearity when survey is relatively narrow, is unable to satisfy needed for the fields such as chemiluminescence immune assay field or the detection of other light energies
The problem of wide range of linearity measures, provides a kind of photon measurement system based on the wide range of linearity of two-photon counter.
To solve the above problems, the invention adopts the following technical scheme:
A kind of photon measurement system based on the wide range of linearity of two-photon counter, including lamp guide, light-dividing device, survey it is low
It is worth photon counter, surveys high level photon counter, diaphragm, attenuator and calculation processing apparatus, the light-dividing device is arranged in institute
It states in lamp guide, the survey low value photon counter and the survey high level photon counter connect with the calculation processing apparatus respectively
It connects;
The light that measured object issues is incident to the spectro-film of the light-dividing device through the lamp guide, transmits through the spectro-film
Transmitted light be incident to the survey low value photon counter, the reflected light reflected through the spectro-film is by the diaphragm and described
The survey high level photon counter is incident to after attenuator;
The calculation processing apparatus judges whether the first measured value of the survey high level photon counter output is greater than threshold value, institute
Stating threshold value is the corresponding number of photons for surveying high level photon counter in the linear highest point for surveying low value photon counter, if
It is, then the calculation processing apparatus output photon probe value that the photon detection value is first measured value and the spectro-film
Splitting ratio product.
A kind of above-mentioned photon measurement system based on the wide range of linearity of two-photon counter utilizes survey low value photon counter
It is combined with high level photon counter is surveyed, the wide line range detection of faint light relative light intensity is realized, to meet chemical hair
The demand of wide range of linearity measurement needed for the fields such as light immunoassay field or the detection of other light energies.
Detailed description of the invention
Fig. 1 is that the present invention is based on the schematic illustrations of the photon measurement system of the wide range of linearity of two-photon counter;
Fig. 2 is that the present invention is based on the structural schematic diagrams of the photon measurement system of the wide range of linearity of two-photon counter;
Fig. 3 is that the present invention is based on the range of linearity schematic diagrames of the photon measurement system of the wide range of linearity of two-photon counter.
Specific embodiment
Technical solution of the present invention is described in detail below in conjunction with attached drawing and preferred embodiment.
A kind of photon measurement system based on the wide range of linearity of two-photon counter of the invention in one of the embodiments,
Including lamp guide 1, light-dividing device 2, surveys low value photon counter 3, survey high level photon counter 4, diaphragm 5, attenuator 6 and calculate
Processing unit, wherein light-dividing device 2 is arranged in lamp guide 1, surveys low value photon counter 3 and surveys high level photon counter 4 and divides
It is not connect with calculation processing apparatus.Fig. 1 show the schematic illustration of photon measurement system of the present invention, and Fig. 2 show the present invention
The structural schematic diagram of photon measurement system, Fig. 3 show the range of linearity schematic diagram of photon measurement system of the present invention, in Fig. 3,
Transverse and longitudinal coordinate is all to take denary logarithm value, and in Fig. 2, lamp guide 1 surveys low value photon counter 3, surveys high level photon meter
Number device 4, diaphragm 5 and attenuator 6 are fixed by optical bracket and bolt etc., and survey the setting of low value photon counter 3
The transmitted light exit direction of light-dividing device 2 in lamp guide 1, diaphragm 5, attenuator 6 and survey high level photon counter 4 are set gradually
In the reflected light exit direction of light-dividing device 2.
Specifically, the light that measured object issues is incident to the spectro-film of light-dividing device 2 through lamp guide 1, and lamp guide 1 has leaded light
Effect, avoid measured object issue light dissipated during propagation, light-dividing device 2 have spectro-film, spectro-film can be right
Measured object issue light transmitted and reflected, the light-dividing device 2 in the present embodiment can be Amici prism or light splitting piece,
Spectro-film is coated on Amici prism and light splitting piece, light-dividing device 2 may be that other are coated with the optical element of spectro-film, with light splitting
Prism or light splitting piece are conducive to simplify optical system structure and reduce cost etc. as the light-dividing device 2 in the present embodiment.
The splitting ratio value range of the transmitted light and reflected light of the spectro-film of light-dividing device 2 can be for tens to one to several in the present embodiment
Hundred to one, it is preferable that the splitting ratio of the transmitted light and reflected light of the spectro-film of light-dividing device 2 is 100 in the present embodiment:1, i.e., thoroughly
Penetrating the ratio between energy of light and reflected light is 100:1, low value photon counter 3 and survey high level photon counting are surveyed to advantageously ensure that
The measurement accuracy of device 4.
The transmitted light of spectro-film transmission through light-dividing device 2, which is incident to, surveys low value photon counter 3, through light-dividing device 2
The reflected light of spectro-film reflection is incident to after diaphragm 5 and attenuator 6 surveys high level photon counter 4.Survey low value photon counting
Device 3 and survey high level photon counter 4 are respectively used to receive the transmitted light and reflected light of spectro-film, to obtain corresponding number of photons
Measured value.
Calculation processing apparatus is connect with survey low value photon counter 3 and survey high level photon counter 4 respectively, is obtained and is surveyed low value
Photon counter 3 and the measured value for surveying the number of photons that high level photon counter 4 respectively measures, and judge to survey high level photon counter 4
Whether the first measured value of output is greater than threshold value, which is to survey the corresponding survey high level in linear highest point of low value photon counter 3
The number of photons of photon counter 4, wherein the linear highest point for surveying low value photon counter 3 can be according to survey low value photon counter 3
Relative light intensity and photon number curve determine that and the corresponding number of photons for surveying high level photon counter 4 in linear highest point refers to
Under the same coordinate system, under the corresponding relative light intensity in linear highest point, survey high level photon counter 4 corresponding to the relative light intensity
Relative light intensity and corresponding number of photons on photon number curve;If calculation processing apparatus determines survey, high level photon counter 4 is defeated
The first measured value out is greater than threshold value, then calculation processing apparatus, by output photon probe value, which is the first survey
The product of value and the splitting ratio of spectro-film.Calculation processing apparatus in the present embodiment can be used based on single-chip microcontroller or comparator
Control circuit is realized.
It is reached at the spectro-film of light-dividing device 2 for example, the light that measured object issues enters lamp guide 1, light beam is at spectro-film
It is divided into two parts, a part is transmitted light, and a part is reflected light, and wherein the lower reflected light of energy by diaphragm 5 and declines
It is incident on survey high level photon counter 3 after subtracting piece 4, the higher transmitted light of energy, which is directly incident on, surveys low value photon counter 3
On, two photon counters measure simultaneously, when calculation processing apparatus judges that surveying the first of the output of high level photon counter 4 surveys
When value is greater than threshold value, since the threshold value is to survey the corresponding survey high level photon counter 4 in linear highest point of low value photon counter 3
Number of photons, therefore when survey high level photon counter 4 export the first measured value be greater than threshold value when, shown the luminous value of measured object
Through the range of linearity beyond survey low value photon counter 3, so the first measured value conduct for surveying high level photon counter 4 is enabled at this time
Actual measured value, calculation processing apparatus output photon detection value be high level photon counter 4 export the first measured value multiplied by point
Light ratio, such as when the first measured value for surveying high level photon counter 4 is 10000, it is assumed that the transmitted light of spectro-film and point of reflected light
Light ratio is 100:1, then the photon detection value of calculation processing apparatus output is 10000 × 100=1000000.
A kind of photon measurement system based on the wide range of linearity of two-photon counter that the present embodiment is proposed is low using surveying
Value photon counter and survey high level photon counter combine, and realize the wide line range detection of faint light relative light intensity, from
And meet the demand of wide range of linearity measurement needed for the fields such as chemiluminescence immune assay field or the detection of other light energies.
As a kind of specific embodiment, if calculation processing apparatus determines to survey high level photon counter 4 exports first
Measured value is less than or equal to threshold value, which is to survey the corresponding survey high level photon meter in linear highest point of low value photon counter 3
The number of photons of number device 4, and the second measured value for surveying the output of low value photon counter 3 is corresponding less than or equal to linear highest point
Number of photons shows the luminous value of measured object not departing from the range of linearity for surveying low value photon counter 3, then at this time at calculating
The photon detection value for managing device output is the second measured value surveying low value photon counter 3 and exporting.For example, the light that measured object issues
At the spectro-film for reaching light-dividing device 2 into lamp guide 1, light beam is divided into two parts at spectro-film, and a part is transmitted light,
A part is reflected light, and wherein the lower reflected light of energy is incident on after diaphragm 5 and attenuator 4 surveys high level photon counter
On 3, the higher transmitted light of energy, which is directly incident on, to be surveyed on low value photon counter 3, and two photon counters measure simultaneously,
Assuming that the corresponding number of photons in linear highest point for surveying low value photon counter 3 is a, the corresponding light for surveying high level photon counter 4
Subnumber is b, i.e., threshold value is b, when calculation processing apparatus determines that surveying the first measured value that high level photon counter 4 exports is greater than b, meter
The photon detection value for calculating processing unit output is the product of the first measured value and splitting ratio;When calculation processing apparatus determines to survey high level light
The first measured value that sub-count device 4 exports is less than or equal to b, and the second measured value for surveying the output of low value photon counter 3 is less than
Or when being equal to a, the photon detection value of calculation processing apparatus output is the second measured value.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (4)
1. a kind of photon measurement system based on the wide range of linearity of two-photon counter, which is characterized in that including lamp guide (1),
At light-dividing device (2), survey low value photon counter (3), survey high level photon counter (4), diaphragm (5), attenuator (6) and calculating
Device is managed, in the lamp guide (1), the survey low value photon counter (3) and the survey are high for light-dividing device (2) setting
Value photon counter (4) is connect with the calculation processing apparatus respectively;
The light that measured object issues is incident to the spectro-film of the light-dividing device (2) through the lamp guide (1), saturating through the spectro-film
The transmitted light penetrated is incident to the survey low value photon counter (3), and the reflected light reflected through the spectro-film passes through the diaphragm
(5) and after the attenuator (6) it is incident to the survey high level photon counter (4);
The calculation processing apparatus judges whether the first measured value of survey high level photon counter (4) output is greater than threshold value, institute
Stating threshold value is the corresponding light for surveying high level photon counter (4) in the linear highest point for surveying low value photon counter (3)
Subnumber, if so, the calculation processing apparatus output photon probe value, the photon detection value be first measured value with it is described
The product of the splitting ratio of spectro-film.
2. a kind of photon measurement system based on the wide range of linearity of two-photon counter according to claim 1, feature
It is,
If first measured value is less than or equal to the threshold value, and second measured value surveying low value photon counter 3 and exporting
Number of photons corresponding less than or equal to the linear highest point, then the photon detection value is second measured value.
3. a kind of photon measurement system based on the wide range of linearity of two-photon counter according to claim 1 or 2, special
Sign is,
The transmitted light of the spectro-film and the splitting ratio of reflected light are 100:1.
4. a kind of photon measurement system based on the wide range of linearity of two-photon counter according to claim 1 or 2, special
Sign is,
The light-dividing device 2 is Amici prism or light splitting piece.
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