CN108804874B - 基于分子标记的免疫组库生物信息分析方法 - Google Patents
基于分子标记的免疫组库生物信息分析方法 Download PDFInfo
- Publication number
- CN108804874B CN108804874B CN201810618023.7A CN201810618023A CN108804874B CN 108804874 B CN108804874 B CN 108804874B CN 201810618023 A CN201810618023 A CN 201810618023A CN 108804874 B CN108804874 B CN 108804874B
- Authority
- CN
- China
- Prior art keywords
- umi
- immunogene
- sequence
- gene
- cycle tests
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004458 analytical method Methods 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000002372 labelling Methods 0.000 title claims abstract description 10
- 238000012163 sequencing technique Methods 0.000 claims abstract description 36
- 238000012360 testing method Methods 0.000 claims abstract description 34
- 238000012937 correction Methods 0.000 claims abstract description 14
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 12
- 125000002015 acyclic group Chemical group 0.000 claims abstract description 6
- 238000012408 PCR amplification Methods 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 34
- 102000018358 immunoglobulin Human genes 0.000 claims description 10
- 108700005091 Immunoglobulin Genes Proteins 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 229940099472 immunoglobulin a Drugs 0.000 claims description 4
- 230000002950 deficient Effects 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 238000003908 quality control method Methods 0.000 claims description 3
- 101150107439 CDR3 gene Proteins 0.000 claims description 2
- 101150097493 D gene Proteins 0.000 claims description 2
- 101150013191 E gene Proteins 0.000 claims description 2
- 101150082239 G gene Proteins 0.000 claims description 2
- 229940027941 immunoglobulin g Drugs 0.000 claims description 2
- 108020005038 Terminator Codon Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 10
- 238000002649 immunization Methods 0.000 description 8
- 230000003053 immunization Effects 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 238000002560 therapeutic procedure Methods 0.000 description 8
- 229940072221 immunoglobulins Drugs 0.000 description 5
- 238000012827 research and development Methods 0.000 description 5
- 210000001744 T-lymphocyte Anatomy 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000012165 high-throughput sequencing Methods 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 4
- 210000003719 b-lymphocyte Anatomy 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 238000004393 prognosis Methods 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000009946 DNA mutation Effects 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 238000004422 calculation algorithm Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 101150098072 20 gene Proteins 0.000 description 1
- 102000006306 Antigen Receptors Human genes 0.000 description 1
- 108010083359 Antigen Receptors Proteins 0.000 description 1
- 101100112922 Candida albicans CDR3 gene Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 230000004721 adaptive immunity Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012350 deep sequencing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810618023.7A CN108804874B (zh) | 2018-06-15 | 2018-06-15 | 基于分子标记的免疫组库生物信息分析方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810618023.7A CN108804874B (zh) | 2018-06-15 | 2018-06-15 | 基于分子标记的免疫组库生物信息分析方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108804874A CN108804874A (zh) | 2018-11-13 |
CN108804874B true CN108804874B (zh) | 2019-04-23 |
Family
ID=64086190
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810618023.7A Active CN108804874B (zh) | 2018-06-15 | 2018-06-15 | 基于分子标记的免疫组库生物信息分析方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108804874B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110853709B (zh) * | 2020-01-15 | 2020-06-19 | 求臻医学科技(北京)有限公司 | 一种可以有效降低错误的umi设计方法 |
CN111599411B (zh) * | 2020-06-08 | 2023-04-14 | 谱天(天津)生物科技有限公司 | 一种检测血液bcr重链和轻链的引物及免疫组库方法、应用 |
CN112852936A (zh) * | 2020-06-24 | 2021-05-28 | 广州华银健康医疗集团股份有限公司 | 一种应用免疫组库测序方法分析样本淋巴细胞或浆细胞的方法及其应用及其试剂盒 |
CN113621609A (zh) * | 2021-09-15 | 2021-11-09 | 深圳泛因医学有限公司 | 文库构建引物组及其在高通量检测中的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102212888A (zh) * | 2011-03-17 | 2011-10-12 | 靳海峰 | 一种基于高通量测序的免疫组库构建方法 |
CN104263818A (zh) * | 2014-09-02 | 2015-01-07 | 武汉凯吉盈科技有限公司 | 基于高通量测序技术的全血免疫组库探测方法 |
US9394567B2 (en) * | 2008-11-07 | 2016-07-19 | Adaptive Biotechnologies Corporation | Detection and quantification of sample contamination in immune repertoire analysis |
CN106156536A (zh) * | 2015-04-15 | 2016-11-23 | 深圳华大基因科技有限公司 | 对样本免疫组库测序数据进行处理的方法和系统 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017117358A1 (en) * | 2015-12-30 | 2017-07-06 | Bio-Rad Laboratories, Inc. | Digital protein quantification |
-
2018
- 2018-06-15 CN CN201810618023.7A patent/CN108804874B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9394567B2 (en) * | 2008-11-07 | 2016-07-19 | Adaptive Biotechnologies Corporation | Detection and quantification of sample contamination in immune repertoire analysis |
CN102212888A (zh) * | 2011-03-17 | 2011-10-12 | 靳海峰 | 一种基于高通量测序的免疫组库构建方法 |
CN104263818A (zh) * | 2014-09-02 | 2015-01-07 | 武汉凯吉盈科技有限公司 | 基于高通量测序技术的全血免疫组库探测方法 |
CN106156536A (zh) * | 2015-04-15 | 2016-11-23 | 深圳华大基因科技有限公司 | 对样本免疫组库测序数据进行处理的方法和系统 |
Non-Patent Citations (1)
Title |
---|
Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires;Simon Friedensohn et al.;《Trends in Biotechnology》;20170331;第35卷(第3期);第203-214页 |
Also Published As
Publication number | Publication date |
---|---|
CN108804874A (zh) | 2018-11-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108804874B (zh) | 基于分子标记的免疫组库生物信息分析方法 | |
Sundquist et al. | Whole-genome sequencing and assembly with high-throughput, short-read technologies | |
CN105389481B (zh) | 一种三代全长转录组中可变剪切体的检测方法 | |
Gerritsen et al. | RTCR: a pipeline for complete and accurate recovery of T cell repertoires from high throughput sequencing data | |
Moret et al. | Phylogenetic networks: modeling, reconstructibility, and accuracy | |
CN104298892B (zh) | 基因融合的检测装置和方法 | |
Yermanos et al. | Comparison of methods for phylogenetic B-cell lineage inference using time-resolved antibody repertoire simulations (AbSim) | |
US20050079504A1 (en) | Method and apparatus for mRNA assembly | |
CN110473594A (zh) | 病原微生物基因组数据库及其建立方法 | |
Kim et al. | Deep sequencing of B cell receptor repertoire | |
CN111477281B (zh) | 基于系统进化树的泛基因组构建方法和构建装置 | |
Mirsky et al. | Antibody-specific model of amino acid substitution for immunological inferences from alignments of antibody sequences | |
CN114008712A (zh) | 用于蛋白质工程和生产的方法和系统 | |
EP3084426A1 (en) | Iterative clustering of sequence reads for error correction | |
CN107779495A (zh) | T细胞抗原受体多样性测序文库的构建方法以及试剂盒 | |
CN109411020A (zh) | 利用长测序读段进行全基因组序列补洞的方法 | |
CN112086131A (zh) | 一种高通量测序中假阳性变异位点的筛选方法 | |
El-Mabrouk et al. | Gene family evolution—an algorithmic framework | |
KR102035285B1 (ko) | Dna 샷건 시퀀싱 또는 rna 전사체 어셈블리를 위한 콘티그 프로파일의 업데이트 방법 및 콘티그 형성 방법 | |
Safonova et al. | IgEvolution: clonal analysis of antibody repertoires | |
Karagiannis et al. | Separation and assembly of deep sequencing data into discrete sub-population genomes | |
WO2020052101A1 (zh) | 基于ngs读段搜索实现序列延伸的虚拟pcr方法 | |
CN102841988A (zh) | 一种对核酸序列信息进行匹配的系统和方法 | |
Doerr et al. | Small parsimony for natural genomes in the DCJ-indel model | |
Ashlock et al. | Evolving extremal epidemic networks |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Wang Yongsi Inventor after: Li Xuefei Inventor after: Wen Yunjie Inventor after: Dong Shaoling Inventor after: Wang Xiaodan Inventor before: Li Fenxiang Inventor before: Li Xuefei Inventor before: Wang Yongsi Inventor before: Dong Shaoling Inventor before: Wang Xiaodan |
|
CB03 | Change of inventor or designer information | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Analysis method of immunome library biological information based on molecular marker Effective date of registration: 20200326 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER Co.,Ltd. Registration number: Y2020440000056 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210506 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER Co.,Ltd. Registration number: Y2020440000056 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Bioinformatics analysis method of immunobank based on molecular markers Effective date of registration: 20210510 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER Co.,Ltd. Registration number: Y2021980003398 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220617 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER CO.,LTD. Registration number: Y2021980003398 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Molecular marker based Bioinformation Analysis Method of immune group Library Effective date of registration: 20220620 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER CO.,LTD. Registration number: Y2022980008214 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230705 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER CO.,LTD. Registration number: Y2022980008214 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Molecular Markers Based Bioinformatics Analysis Method for Immune Library Effective date of registration: 20230707 Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER CO.,LTD. Registration number: Y2023980047704 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER CO.,LTD. Registration number: Y2023980047704 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Molecular Markers Based Bioinformatics Analysis Method for Immunohistochemical Libraries Granted publication date: 20190423 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGZHOU HUAYIN MEDICAL LABORATORY CENTER CO.,LTD. Registration number: Y2024980023183 |