CN108802235B - A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole - Google Patents
A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole Download PDFInfo
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- CN108802235B CN108802235B CN201810740406.1A CN201810740406A CN108802235B CN 108802235 B CN108802235 B CN 108802235B CN 201810740406 A CN201810740406 A CN 201810740406A CN 108802235 B CN108802235 B CN 108802235B
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- hole
- discharge nozzle
- pipe connections
- center
- central axis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/42—Flow patterns using counter-current
Abstract
The invention discloses the processing method of a kind of high-capacity and high-speed countercurrent chromatography separation column and through-hole, including socket main body, the side of socket main body is equipped with central axis, is internally provided with center siphunculus, center siphunculus is flexibly connected with central axis；Socket body side is equipped with charging pipe connections, discharge pipe connections, it is also wound with around column tube thereon, around the both ends of column tube respectively with charging pipe connections, discharge pipe connections connection, into, discharging pipe connections on be also respectively connected into, discharge nozzle, into, discharge nozzle sequentially passes through fixing piece, threading hole, center siphunculus and central axis, fixing piece to it is fixed into, the position of discharge nozzle, threading hole is made of straight hole and inclined hole, be machined on the siphunculus of center convenient for into, the first hole and the second hole that discharge nozzle passes through respectively, first hole and the second hole are symmetrical arranged and with center siphunculus surface at 30-40 degree, feed pipe, around column tube, discharge nozzle is sequentially communicated.Realize splitter its reliable unwinding into and out of expects pipe under high speed rotation.
Description
Technical field
The present invention relates to high-speed countercurrent chromatography field, specifically a kind of high-capacity and high-speed countercurrent chromatography separation column and
The processing method of through-hole.
Background technique
High-speed countercurrent chromatography (high speed countercurrent chromatography, abbreviation HSCCC)
It is a kind of liquid luquid partition chromatography isolation technics of the continuous high-efficient to grow up in the 120's of 20th century, it is carried without any solid-state
Body or support.In recent years, natural, green, safety already become the newest health idea that people pursue, therefore this novel separation is pure
Change technology has been to be concerned by more and more people, and current HSCCC is widely used in biomedicine, biochemistry, food, natural products, ring
The multiple fields such as border analysis, become the mainstay of China's modern science and technology and economic construction.
HSCCC has great advantage in terms of separation, has boundless application prospect, but just at present both at home and abroad in the neck
From the point of view of the present Research in domain, there are still many problems to need to analyze and solve.Such as lacks and sample diffusion influence factor is ground
Study carefully；Separating capacity and low efficiency；Column effect theory of shortage system etc..Specifically how in HSCCC feed pipe and discharge nozzle are realized
The problem of reliable unwinding.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of high-capacity and high-speed countercurrent chromatography separation columns
And the processing method of through-hole, realize reliable unwinding when feed pipe and discharge nozzle work in HSCCC.
The purpose of the present invention is achieved through the following technical solutions: a kind of high-capacity and high-speed counter-current chromatograph separation
Column, including socket main body, the side of the socket main body are equipped with central axis, and socket body interior is equipped with center siphunculus, it is described in
Heart siphunculus is flexibly connected with central axis；The side wall of the socket main body is equipped with charging pipe connections, discharging pipe connections, socket
It is also wound in main body around column tube, the both ends around column tube are connect with charging pipe connections, discharging pipe connections respectively, described
Feed pipe, discharge nozzle is also respectively connected on charging pipe connections, discharging pipe connections, the feed pipe, discharge nozzle are successively worn
Fixing piece, threading hole, center siphunculus and central axis are crossed, position of the fixing piece to fixed feed pipe, discharge nozzle is described
Threading hole is made of straight hole and inclined hole, and the first hole passed through respectively convenient for feed pipe, discharge nozzle is machined on the center siphunculus
With the second hole, first hole and the second hole are symmetrical arranged and with center siphunculus surface at 30-40 degree, the feed pipe, around column
Pipe, discharge nozzle are sequentially communicated.
Preferably, if the socket body surfaces are equipped with a threaded hole, the threaded hole is symmetrical arranged.
Preferably, the both ends of the center siphunculus are designed with key, and the central axis is equipped with the keyway being equipped with key,
The big step for being easily installed that key and keyway are cooperatively connected is additionally provided on the center siphunculus, the big step is equipped with sealing gear
Circle.
Preferably, it is described charging pipe connections, discharge pipe connections overall structure be arranged to irregular hexahedron and
It is fixed by screws on the side wall of socket main body.
Preferably, the two of them side of the charging pipe connections, the pipe connections that discharge is equipped with threaded hole, the screw thread
It is equipped in hole to fasten feed pipe, around the fastening bolt of column tube, discharge nozzle.
Preferably, described that adhesive layer is had additional between column tube outermost layer and the shell of socket main body, and between column tube
It also is provided with sealing, is set on the shell of the socket main body there are three threaded hole.
It preferably, further include that motor, belt, belt wheel and the central axis b with setting substantially symmetrical about its central axis, the motor pass through
Belt band movable belt pulley rotates, and the belt wheel connect with central axis b and to drive splitter to rotate.
A kind of processing method of through holes, high-capacity and high-speed countercurrent chromatography separation column include center siphunculus, the center siphunculus
Including the first hole and the second hole, first hole and the second hole machined step:
S1. Selection Center siphunculus surface is benchmark face；
S2. two symmetrical through-holes are chosen at 30-40 degree with datum level；
S3. in S2 two symmetrical through-holes are deviated 10 degree toward two sides respectively with the axis of center siphunculus, is punched, finally
Form the first hole and the second hole.
Working principle of the present invention: feed pipe enters the center siphunculus inside splitter from central axis, then through the first hole, from
Center siphunculus successively passes through straight hole, inclined hole, then enters in charging pipe connections through fixing piece, finally connects with setting in feed pipe
On fitting the other side around column tube be connected to, around column tube around socket main body wind, until winding after, be connected to out around column tube
The side of expects pipe connector, discharge nozzle are connected to by the other side of discharging pipe connections with around column tube, and discharge nozzle successively passes through again
Then fixing piece, inclined hole, straight hole are again introduced into center siphunculus from the second hole, then go out from center siphunculus and central axis connectivity part, into
Enter central axis, is finally connect with external detection device.Realize splitter its reliable solution into and out of expects pipe under high speed rotation
Around.
The beneficial effects of the present invention are:
1. realizing feed pipe by setting charging pipe connections, discharging pipe connections, being connected between column tube, discharge nozzle
Sealing with fastening feed pipe, around the effect of column tube, discharge nozzle；
2. feed pipe, discharge nozzle sequentially pass through fixing piece, threading hole, center siphunculus and central axis, fixing piece are fixed
Into and out of the position of expects pipe, threading hole is made of straight hole and inclined hole, easy to process, is designed beautifully, and is machined with first on the siphunculus of center
Hole and the second hole, the first hole and the second hole are symmetrical arranged and with center siphunculus surface at 30-40 degree, finally realize feed pipe,
Discharge nozzle facilitates cabling；
3. by charging pipe connections, discharging pipe connections on special bolts are set, realize feed pipe, around column tube,
The fastening of discharge nozzle；
4. the present invention realizes splitter its reliable unwinding into and out of expects pipe under high speed rotation.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention a；
Fig. 2 is A direction structure schematic diagram in Fig. 1；
Fig. 3 is the structural schematic diagram of present invention charging pipe connections, the pipe connections that discharge；
Fig. 4 is the structural schematic diagram of center siphunculus of the present invention；
Fig. 5 is the cross-sectional view in the direction B in Fig. 4；
Fig. 6 is structural schematic diagram b of the invention.
Specific embodiment
Technical solution of the present invention is described in further detail with reference to the accompanying drawing, but protection scope of the present invention is not limited to
It is as described below.
Embodiment
As shown in Fig. 1,2,4,5, a kind of high-capacity and high-speed countercurrent chromatography separation column, including socket main body 1, the socket
The side of main body 1 is equipped with central axis 2, and central axis 2 drives socket main body 1 to rotate.Center siphunculus 5 is equipped with inside socket main body 1, in
The both ends of heart siphunculus 5 are designed with key, and the central axis 2 is equipped with the keyway being equipped with key, realize center siphunculus 5 and center
Axis 2 is flexibly connected, and is also machined with the first hole 51 and the second hole passed through respectively convenient for feed pipe 3, discharge nozzle 4 on center siphunculus 5
52, first hole 51 and the second hole 52 are symmetrical arranged and with 5 surface of center siphunculus at 30-40 degree, and feed pipe 3 passes through the first hole
51, discharge nozzle 4 passes through the second hole 52, and in splitter work, feed pipe 3, discharge nozzle 4 are each passed through the first hole 51, the second hole
52 and two hose do not interfere with each other.The big step 51 for being easily installed that key and keyway are cooperatively connected is additionally provided on center siphunculus 5, it is described big
Step 51 is equipped with Sealing shield ring, realizes the sealed connection of center siphunculus 5 and central axis 2, prevents its junction oil leak.
As shown in Figure 1, 2, 3, the side wall of socket main body 1 is equipped with charging pipe connections 11, discharging pipe connections 13, charging
Pipe connections 11, discharging pipe connections 13 realize that feed pipe 3, the sealing connected between column tube 9, discharge nozzle 4 and fastening are fed
Pipe 3, around the effect of column tube 9, discharge nozzle 4.Specifically, it is wound in socket main body 1 around column tube 9, the both ends around column tube 9
13 are connect with charging pipe connections 11, discharging pipe connections respectively, is gone back on the charging pipe connections 11, discharging pipe connections 13
It is connected separately with feed pipe 3, discharge nozzle 4, the feed pipe 3, discharge nozzle 4 sequentially pass through fixing piece 6, threading hole 7, center siphunculus
5 and central axis 2, position of the fixing piece 6 to fixed feed pipe 3, discharge nozzle 4, the threading hole 7 is by straight hole 71 and tiltedly
Hole 72 forms, and the feed pipe 3 is sequentially communicated around column tube 9, discharge nozzle 4.Fixing piece 6 realizes feed pipe 3, discharge nozzle 4
Fixed, when preventing separation column work, feed pipe 3, discharge nozzle 4 are thrown out of.Threading hole 7 is made of straight hole 71 and inclined hole 72, wherein
Clear opening 71 is first beaten above 1 inner pipe wall of socket main body, then beats inclined hole 72, inclined hole 72 and 71 phase of straight hole from outer steps face
It is logical, it is easy to process, it is designed beautifully and realizes feed pipe 3, discharge nozzle 4 facilitates cabling.
Specifically, 1 surface of socket main body is equipped with the threaded hole that 40 diameters are 7mm, and the threaded hole is symmetrical arranged.One
Aspect reduces overall weight, can accurately add screw thread when the another aspect later period does experiment of dynamic balancing to meet balance.
As shown in figure 3, more specifically, the charging pipe connections 11, the pipe connections 13 that discharge overall structure set
It is set to irregular hexahedron and is fixed by screws on the side wall of socket main body 1.The charging pipe connections 11, discharge nozzle
The two of them side of connector 13 is equipped with threaded hole, is equipped in the threaded hole to fasten feed pipe 3, around column tube 9, discharging
The fastening bolt of pipe 4.
Furthermore, it is understood that described have additional adhesive layer between 9 outermost layer of column tube and the shell of socket main body 1, and around column
It also is provided with sealing between pipe 9, for fixed around column tube 9, prevents its sliding.It is set on the shell of socket main body 1 there are three threaded hole,
For sealing.
As shown in fig. 6, furthermore, it is understood that further include motor 14, belt 15, belt wheel 16 and with central axis 2 be symmetrical arranged
Central axis b10, central axis b10 and central axis 2 be separately positioned on the two sides of socket main body 1, and the motor 14 passes through belt 15
Band movable belt pulley 16 rotates, and the belt wheel 16 is connect and to drive splitter to rotate with central axis b10.Pass through belt wheel with motor 14
16, central axis b10 drives splitter work.
As shown in Figure 4,5, a kind of processing method of through holes, high-capacity and high-speed countercurrent chromatography separation column include center siphunculus 5,
The center siphunculus 5 includes the first hole 51 and the second hole 52,52 procedure of processing of first hole 51 and the second hole:
S1. 5 surface of Selection Center siphunculus is benchmark face；
S2. two symmetrical through-holes are chosen at 30-40 degree with datum level；
S3. in S2 two symmetrical through-holes are deviated 10 degree toward two sides respectively with the axis of center siphunculus 5, is punched, most
End form is at the first hole 51 and the second hole 52.The first hole 51 and the second hole 52 after processing, due to shifting to install, so that splitter work
When making, feed pipe 3, discharge nozzle 4 are independent of each other, and are convenient for its unwinding.
The above is only a preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form should not be regarded as an exclusion of other examples, and can be used for other combinations, modifications, and environments, and can be at this
In the text contemplated scope, modifications can be made through the above teachings or related fields of technology or knowledge.And those skilled in the art institute into
Capable modifications and changes do not depart from the spirit and scope of the present invention, then all should be in the protection scope of appended claims of the present invention
It is interior.
Claims (8)
1. a kind of high-capacity and high-speed countercurrent chromatography separation column, which is characterized in that including socket main body, the one of the socket main body
Side is equipped with central axis, and socket body interior is equipped with center siphunculus, and the center siphunculus is flexibly connected with central axis；The socket master
The side wall of body is equipped with charging pipe connections, discharging pipe connections, is also wound with around column tube in socket main body, described around column tube
Both ends are connect with charging pipe connections, discharging pipe connections respectively, are also distinguished on the charging pipe connections, discharging pipe connections
It is connected with feed pipe, discharge nozzle, the feed pipe, discharge nozzle sequentially pass through fixing piece, threading hole, center siphunculus and center
Axis, position of the fixing piece to fixed feed pipe, discharge nozzle, the threading hole are made of straight hole and inclined hole, the center
The first hole and the second hole passed through respectively convenient for feed pipe, discharge nozzle is machined on siphunculus, first hole and the second hole are symmetrical
It is arranged and with center siphunculus surface at 30-40 degree, the feed pipe is sequentially communicated around column tube, discharge nozzle.
2. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that: the socket master
If body surface face is equipped with a threaded hole, the threaded hole is symmetrical arranged.
3. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that: the center is logical
The both ends of pipe are designed with key, and the central axis is equipped with the keyway being equipped with key, is additionally provided with and is convenient on the center siphunculus
The big step that installation key and keyway are cooperatively connected, the big step are equipped with Sealing shield ring.
4. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that: the feed pipe
Connector, discharge pipe connections overall structure be arranged to irregular hexahedron and be fixed by screws in the side of socket main body
On wall.
5. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1 or 4, it is characterised in that: it is described into
Expects pipe connector, the pipe connections that discharge two of them side be equipped with threaded hole, be equipped with to fasten charging in the threaded hole
It manages, around the fastening bolt of column tube, discharge nozzle.
6. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that: described around column tube
Adhesive layer is had additional between outermost layer and the shell of socket main body, and sealing also is provided between column tube, the socket main body
Threaded hole there are three being set on shell.
7. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that: further include electricity
Machine, belt, belt wheel and the central axis b with setting substantially symmetrical about its central axis, the motor are rotated by belt band movable belt pulley, the band
Wheel is connect and to drive splitter to rotate with central axis b.
8. a kind of through-hole processing side for high-capacity and high-speed countercurrent chromatography separation column of any of claims 1-7
Method, it is characterised in that: first hole and the second hole machined step:
S1. Selection Center siphunculus surface is benchmark face；
S2. two symmetrical through-holes are chosen at 30-40 degree with datum level；
S3. two symmetrical through-holes in step S2 are deviated 10 degree toward two sides respectively with the axis of center siphunculus, is punched, finally
Form the first hole and the second hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810740406.1A CN108802235B (en) | 2018-07-07 | 2018-07-07 | A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810740406.1A CN108802235B (en) | 2018-07-07 | 2018-07-07 | A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole |
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| Publication Number | Publication Date |
|---|---|
| CN108802235A CN108802235A (en) | 2018-11-13 |
| CN108802235B true CN108802235B (en) | 2019-11-12 |
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| CN201810740406.1A Active CN108802235B (en) | 2018-07-07 | 2018-07-07 | A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110960886B (en) * | 2019-11-11 | 2020-09-04 | 四川大学 | High-speed counter-current chromatographic separation column |
| CN112345677B (en) * | 2020-11-09 | 2021-10-22 | 四川大学 | Single-side self-unwinding high-speed counter-current chromatograph with double separation columns |
| CN115469047B (en) * | 2022-09-21 | 2023-08-15 | 四川大学华西医院 | Countercurrent chromatographic separation column without adapter |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3856669A (en) * | 1973-07-02 | 1974-12-24 | Department Of Health Education | Elution centrifuge-apparatus and method |
| US5024758A (en) * | 1988-12-20 | 1991-06-18 | The United States Of America As Represented By The Department Of Health And Human Services | Horizontal flow-through coil planet centrifuge with multilayer plural coils in eccentric synchronous rotation, suitable for counter-current chromatography |
| CN1405559A (en) * | 2002-10-25 | 2003-03-26 | 浙江大学 | Vertical high-speed counter-flow chromatograph |
| CN2670945Y (en) * | 2003-12-29 | 2005-01-12 | 杜琪珍 | Improved counter current cromatographic apparatus |
| CN2781368Y (en) * | 2005-03-24 | 2006-05-17 | 中国农业科学院茶叶研究所 | Large capacity counter-flow chromatograph dewind by mechanical device |
| CN206192959U (en) * | 2016-12-02 | 2017-05-24 | 山东省分析测试中心 | Novel but counter weight counter current chromatography separator column |
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2018
- 2018-07-07 CN CN201810740406.1A patent/CN108802235B/en active Active
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Inventor after: Yin Guofu Inventor after: Chen Haijun Inventor after: Chen Jianfeng Inventor after: Yin Qin Inventor after: Chen Lijuan Inventor after: Peng Aihua Inventor before: Yin Guofu Inventor before: Chen Haijun Inventor before: Chen Jianfeng Inventor before: Yin Qin Inventor before: Chen Lijuan Inventor before: Peng Aihua |
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