CN108802235A - A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole - Google Patents
A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole Download PDFInfo
- Publication number
- CN108802235A CN108802235A CN201810740406.1A CN201810740406A CN108802235A CN 108802235 A CN108802235 A CN 108802235A CN 201810740406 A CN201810740406 A CN 201810740406A CN 108802235 A CN108802235 A CN 108802235A
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- China
- Prior art keywords
- hole
- discharge nozzle
- center
- siphunculus
- pipe connections
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/42—Flow patterns using counter-current
Abstract
The invention discloses the processing method of a kind of high-capacity and high-speed countercurrent chromatography separation column and through-hole, including socket main body, the side of socket main body is equipped with central shaft, is internally provided with center siphunculus, center siphunculus is flexibly connected with central shaft；Socket body side is equipped with charging pipe connections, discharge pipe connections, it is also wound with around column tube thereon, around the both ends of column tube respectively with charging pipe connections, discharge pipe connections connection, into, discharging pipe connections on be also respectively connected into, discharge nozzle, into, discharge nozzle sequentially passes through fixing piece, threading hole, center siphunculus and central shaft, fixing piece to it is fixed into, the position of discharge nozzle, threading hole is made of straight hole and inclined hole, be machined on the siphunculus of center convenient for into, discharge nozzle respectively by the first hole and the second hole, first hole is symmetrical arranged with the second hole and with center siphunculus surface at 30-40 degree, feed pipe, around column tube, discharge nozzle is sequentially communicated.Realize splitter its reliable unwinding into and out of expects pipe under high speed rotation.
Description
Technical field
The present invention relates to high-speed countercurrent chromatography field, specifically a kind of high-capacity and high-speed countercurrent chromatography separation column and
The processing method of through-hole.
Background technology
High-speed countercurrent chromatography (high speed countercurrent chromatography, abbreviation HSCCC)
It is a kind of liquid luquid partition chromatography isolation technics of the continuous high-efficient to grow up in the 120's of 20th century, it is carried without any solid-state
Body or support.In recent years, natural, green, safety already become the newest health idea that people pursue, therefore this novel separation is pure
Change technology has been to be concerned by more and more people, and current HSCCC is widely used in biomedicine, biochemistry, food, natural products, ring
The multiple fields such as border analysis, become China's modern science and technology and the mainstay of economic construction.
HSCCC has great advantage in terms of separation, has boundless application prospect, but just at present both at home and abroad in the neck
From the point of view of the present Research in domain, needs to analyze and solve there are still many problems.Such as lacks and sample diffusion influence factor is ground
Study carefully；Separating capacity and efficiency are low；Column effect theory of shortage system etc..Specifically how in HSCCC feed pipe and discharge nozzle are realized
The problem of reliable unwinding.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of high-capacity and high-speed countercurrent chromatography separation columns
And the processing method of through-hole, realize reliable unwinding when feed pipe works with discharge nozzle in HSCCC.
The purpose of the present invention is achieved through the following technical solutions：A kind of high-capacity and high-speed counter-current chromatograph separation
The side of column, including socket main body, the socket main body is equipped with central shaft, and socket body interior is equipped with center siphunculus, in described
Heart siphunculus is flexibly connected with central shaft；The side wall of the socket main body is equipped with charging pipe connections, discharging pipe connections, socket
It is also wound in main body around column tube, it is described to be connect respectively with charging pipe connections, discharging pipe connections around the both ends of column tube, it is described
Feed pipe, discharge nozzle is also respectively connected on charging pipe connections, discharging pipe connections, the feed pipe, discharge nozzle are worn successively
Fixing piece, threading hole, center siphunculus and central shaft are crossed, the fixing piece is described to fix the position of feed pipe, discharge nozzle
Threading hole is made of straight hole and inclined hole, be machined on the center siphunculus convenient for feed pipe, discharge nozzle respectively by the first hole
With the second hole, first hole and the second hole are symmetrical arranged and with center siphunculus surface at 30-40 degree, the feed pipe, around column
Pipe, discharge nozzle are sequentially communicated.
Preferably, if the socket body surfaces are equipped with a threaded hole, the threaded hole is symmetrical arranged.
Preferably, the both ends of the center siphunculus are designed with key, and the central shaft is equipped with the keyway being equipped with key,
It is additionally provided on the center siphunculus and is easily installed the big step that key is connected with keyway, the big step is equipped with sealing and keeps off
Circle.
Preferably, it is described charging pipe connections, discharge pipe connections overall structure be arranged to irregular hexahedron and
It is fixed by screws on the side wall of socket main body.
Preferably, the two of which side of the charging pipe connections, the pipe connections that discharge is equipped with threaded hole, the screw thread
It is equipped with to fasten feed pipe, around the fastening bolt of column tube, discharge nozzle in hole.
Preferably, described that adhesive layer is had additional between column tube outermost layer and the shell of socket main body, and between column tube
It also is provided with sealing, is set on the shell of the socket main body there are three threaded hole.
Preferably, further include that motor, belt, belt wheel and the central shaft b with setting substantially symmetrical about its central axis, the motor pass through
Belt band movable belt pulley rotates, and the belt wheel connect with central shaft b and to drive splitter to rotate.
A kind of processing method of through holes, high-capacity and high-speed countercurrent chromatography separation column include center siphunculus, the center siphunculus
Including the first hole and the second hole, first hole and the second hole machined step：
S1. face on the basis of Selection Center siphunculus surface；
S2. two symmetrical through-holes are chosen at 30-40 degree with reference plane；
S3. in S2 two symmetrical through-holes are deviated 10 degree toward both sides respectively with the axis of center siphunculus, is punched, is ultimately formed
First hole and the second hole.
Operation principle of the present invention：Feed pipe enters the center siphunculus inside splitter from central shaft, then through the first hole, from
Center siphunculus passes through straight hole, inclined hole successively, then enters in charging pipe connections through fixing piece, finally connects in feed pipe with setting
On fitting the other side around column tube be connected to, around column tube around socket main body wind, until winding after, be connected to out around column tube
The side of expects pipe connector, discharge nozzle are connected to by the other side for the pipe connections that discharge with around column tube, and discharge nozzle passes through successively again
Then fixing piece, inclined hole, straight hole are again introduced into center siphunculus from the second hole, then go out from center siphunculus and central shaft connectivity part, into
Enter central shaft, is finally connect with external detection device.Realize splitter its reliable solution into and out of expects pipe under high speed rotation
Around.
The beneficial effects of the invention are as follows：
1. by setting feed pipe connections, discharging pipe connections, realize feed pipe, connected between column tube, discharge nozzle it is close
It seals with fastening feed pipe, around the effect of column tube, discharge nozzle；
2. feed pipe, discharge nozzle sequentially pass through fixing piece, threading hole, center siphunculus and central shaft, fixing piece fix into and out of
The position of expects pipe, threading hole are made of straight hole and inclined hole, easy to process, are designed beautifully, be machined on the siphunculus of center the first hole with
Second hole, the first hole are symmetrical arranged with the second hole and with center siphunculus surface at 30-40 degree, finally realize feed pipe, discharging
Pipe facilitates cabling；
3. by the way that special bolts are arranged on charging pipe connections, discharging pipe connections, feed pipe is realized, around column tube, discharging
The fastening of pipe；
4. the present invention realizes splitter its reliable unwinding into and out of expects pipe under high speed rotation.
Description of the drawings
Fig. 1 is the structural diagram of the present invention a；
Fig. 2 is A direction structure schematic diagrames in Fig. 1；
Fig. 3 is the structural schematic diagram of present invention charging pipe connections, the pipe connections that discharge；
Fig. 4 is the structural schematic diagram of center siphunculus of the present invention；
Fig. 5 is the sectional view in the directions B in Fig. 4；
Fig. 6 is the structural schematic diagram b of the present invention.
Specific implementation mode
Technical scheme of the present invention is described in further detail below in conjunction with the accompanying drawings, but protection scope of the present invention is not limited to
It is as described below.
Embodiment
As shown in Fig. 1,2,4,5, a kind of high-capacity and high-speed countercurrent chromatography separation column, including socket main body 1, the socket
The side of main body 1 is equipped with central shaft 2, and central shaft 2 drives socket main body 1 to rotate.Socket main body 1 is internally provided with center siphunculus 5, in
The both ends of heart siphunculus 5 are designed with key, and the central shaft 2 is equipped with the keyway being equipped with key, realizes center siphunculus 5 and center
Axis 2 is flexibly connected, be also machined on center siphunculus 5 convenient for feed pipe 3, discharge nozzle 4 respectively by the first hole 51 and the second hole
52, first hole, 51 and second hole 52 is symmetrical arranged and with 5 surface of center siphunculus at 30-40 degree, and feed pipe 3 passes through the first hole
51, discharge nozzle 4 passes through the second hole 52, and when splitter works, feed pipe 3, discharge nozzle 4 are each passed through the first hole 51, the second hole
52 and two hose do not interfere with each other.It is additionally provided on center siphunculus 5 and is easily installed the big step 51 that key is connected with keyway, it is described big
Step 51 is equipped with Sealing shield ring, realizes the sealed connection of center siphunculus 5 and central shaft 2, prevents its junction oil leak.
As shown in Figure 1, 2, 3, the side wall of socket main body 1 is equipped with charging pipe connections 11, discharging pipe connections 13, charging
Sealing and the fastening charging that pipe connections 11, discharging pipe connections 13 are realized feed pipe 3, connected between column tube 9, discharge nozzle 4
Pipe 3, around the effect of column tube 9, discharge nozzle 4.Specifically, it is wound in socket main body 1 around column tube 9, it is described around the both ends of column tube 9
13 are connect with charging pipe connections 11, discharging pipe connections respectively, is gone back on the charging pipe connections 11, discharging pipe connections 13
It is connected separately with feed pipe 3, discharge nozzle 4, the feed pipe 3, discharge nozzle 4 sequentially pass through fixing piece 6, threading hole 7, center siphunculus
5 and central shaft 2, the fixing piece 6 is to the position of fixed feed pipe 3, discharge nozzle 4, and the threading hole 7 is by straight hole 71 and tiltedly
Hole 72 forms, and the feed pipe 3 is sequentially communicated around column tube 9, discharge nozzle 4.Fixing piece 6 realizes feed pipe 3, discharge nozzle 4
Fixed, when preventing splitter from working, feed pipe 3, discharge nozzle 4 are thrown out of.Threading hole 7 is made of straight hole 71 and inclined hole 72, wherein
Clear opening 71 is first beaten above 1 inner pipe wall of socket main body, then beats inclined hole 72, inclined hole 72 and 71 phase of straight hole from outer steps face
It is logical, it is easy to process, it is designed beautifully and realizes feed pipe 3, discharge nozzle 4 facilitates cabling.
Specifically, 1 surface of socket main body is equipped with the threaded hole of 40 a diameter of 7mm, and the threaded hole is symmetrical arranged.One
Aspect reduces overall weight, and screw thread can be accurately added to meet balance when the another aspect later stage does experiment of dynamic balancing.
As shown in figure 3, more specifically, the charging pipe connections 11, the pipe connections 13 that discharge overall structure set
It is set to irregular hexahedron and is fixed by screws on the side wall of socket main body 1.The charging pipe connections 11, discharge nozzle
The two of which side of connector 13 is equipped with threaded hole, is equipped in the threaded hole to fasten feed pipe 3, around column tube 9, discharging
The fastening bolt of pipe 4.
Furthermore, it is understood that described have additional adhesive layer between 9 outermost layer of column tube and the shell of socket main body 1, and around column
It also is provided with sealing between pipe 9, for fixed around column tube 9, prevents its sliding.It is set on the shell of socket main body 1 there are three threaded hole,
For sealing.
As shown in fig. 6, furthermore, it is understood that further include motor 14, belt 15, belt wheel 16 and with central shaft 2 be symmetrical arranged
Central shaft b10, central shaft b10 and central shaft 2 be separately positioned on the both sides of socket main body 1, and the motor 14 passes through belt 15
Band movable belt pulley 16 rotates, and the belt wheel 16 connect with central shaft b10 and to drive splitter to rotate.Pass through belt wheel with motor 14
16, central shaft b10 drives splitter work.
As shown in Figure 4,5, a kind of processing method of through holes, high-capacity and high-speed countercurrent chromatography separation column include center siphunculus 5,
The center siphunculus 5 includes the first hole 51 and the second hole 52,51 and second hole of the first hole, 52 procedure of processing：
S1. face on the basis of 5 surface of Selection Center siphunculus；
S2. two symmetrical through-holes are chosen at 30-40 degree with reference plane；
S3. in S2 two symmetrical through-holes are deviated 10 degree toward both sides respectively with the axis of center siphunculus 5, is punched, most end form
At the first hole 51 and the second hole 52.The first hole 51 after processing and the second hole 52, due to shifting to install so that splitter works
When, feed pipe 3, discharge nozzle 4 are independent of each other, and are convenient for its unwinding.
The above is only a preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form is not to be taken as excluding other embodiments, and can be used for other combinations, modifications, and environments, and can be at this
In the text contemplated scope, modifications can be made through the above teachings or related fields of technology or knowledge.And those skilled in the art institute into
Capable modifications and changes do not depart from the spirit and scope of the present invention, then all should be in the protection domain of appended claims of the present invention
It is interior.
Claims (8)
1. a kind of high-capacity and high-speed countercurrent chromatography separation column, which is characterized in that including socket main body, the one of the socket main body
Side is equipped with central shaft, and socket body interior is equipped with center siphunculus, and the center siphunculus is flexibly connected with central shaft；The socket master
The side wall of body is equipped with charging pipe connections, discharging pipe connections, is also wound with around column tube in socket main body, described around column tube
Both ends are connect with charging pipe connections, discharging pipe connections respectively, are also distinguished on the charging pipe connections, discharging pipe connections
It is connected with feed pipe, discharge nozzle, the feed pipe, discharge nozzle sequentially pass through fixing piece, threading hole, center siphunculus and center
Axis, the fixing piece is to fix the position of feed pipe, discharge nozzle, and the threading hole is made of straight hole and inclined hole, the center
Be machined on siphunculus convenient for feed pipe, discharge nozzle respectively by the first hole and the second hole, first hole and the second hole are symmetrical
It is arranged and with center siphunculus surface at 30-40 degree, the feed pipe is sequentially communicated around column tube, discharge nozzle.
2. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that：The socket master
If body surface face is equipped with a threaded hole, the threaded hole is symmetrical arranged.
3. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that：The center is logical
The both ends of pipe are designed with key, and the central shaft is equipped with the keyway being equipped with key, is additionally provided with and is convenient on the center siphunculus
The big step that installation key is connected with keyway, the big step are equipped with Sealing shield ring.
4. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that：The feed pipe
Connector, discharge pipe connections overall structure be arranged to irregular hexahedron and be fixed by screws in the side of socket main body
On wall.
5. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1 or 4, it is characterised in that：It is described into
Expects pipe connector, the pipe connections that discharge two of which side be equipped with threaded hole, be equipped in the threaded hole to fasten charging
It manages, around the fastening bolt of column tube, discharge nozzle.
6. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that：It is described around column tube
Adhesive layer is had additional between outermost layer and the shell of socket main body, and sealing also is provided between column tube, the socket main body
Threaded hole there are three being set on shell.
7. a kind of high-capacity and high-speed countercurrent chromatography separation column according to claim 1, it is characterised in that：It further include electricity
Machine, belt, belt wheel and the central shaft b with setting substantially symmetrical about its central axis, the motor are rotated by belt band movable belt pulley, the band
Wheel is connect with central shaft b and to drive splitter to rotate.
8. a kind of processing method of through holes, it is characterised in that：High-capacity and high-speed countercurrent chromatography separation column includes center siphunculus, described
Center siphunculus includes the first hole and the second hole, first hole and the second hole machined step：
S1. face on the basis of Selection Center siphunculus surface；
S2. two symmetrical through-holes are chosen at 30-40 degree with reference plane；
S3. in S2 two symmetrical through-holes are deviated 10 degree toward both sides respectively with the axis of center siphunculus, is punched, is ultimately formed
First hole and the second hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810740406.1A CN108802235B (en) | 2018-07-07 | 2018-07-07 | A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810740406.1A CN108802235B (en) | 2018-07-07 | 2018-07-07 | A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole |
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| Publication Number | Publication Date |
|---|---|
| CN108802235A true CN108802235A (en) | 2018-11-13 |
| CN108802235B CN108802235B (en) | 2019-11-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810740406.1A Active CN108802235B (en) | 2018-07-07 | 2018-07-07 | A kind of processing method of high-capacity and high-speed countercurrent chromatography separation column and through-hole |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110960886A (en) * | 2019-11-11 | 2020-04-07 | 四川大学 | High-speed counter-current chromatographic separation column |
| CN112345677A (en) * | 2020-11-09 | 2021-02-09 | 四川大学 | Single-side self-unwinding high-speed counter-current chromatograph with double separation columns |
| CN115469047A (en) * | 2022-09-21 | 2022-12-13 | 四川大学华西医院 | Counter-current chromatographic separation column without adapter |
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| US3856669A (en) * | 1973-07-02 | 1974-12-24 | Department Of Health Education | Elution centrifuge-apparatus and method |
| US5024758A (en) * | 1988-12-20 | 1991-06-18 | The United States Of America As Represented By The Department Of Health And Human Services | Horizontal flow-through coil planet centrifuge with multilayer plural coils in eccentric synchronous rotation, suitable for counter-current chromatography |
| CN1405559A (en) * | 2002-10-25 | 2003-03-26 | 浙江大学 | Vertical high-speed counter-flow chromatograph |
| CN2670945Y (en) * | 2003-12-29 | 2005-01-12 | 杜琪珍 | Improved counter current cromatographic apparatus |
| CN2781368Y (en) * | 2005-03-24 | 2006-05-17 | 中国农业科学院茶叶研究所 | Large capacity counter-flow chromatograph dewind by mechanical device |
| CN206192959U (en) * | 2016-12-02 | 2017-05-24 | 山东省分析测试中心 | Novel but counter weight counter current chromatography separator column |
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2018
- 2018-07-07 CN CN201810740406.1A patent/CN108802235B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3856669A (en) * | 1973-07-02 | 1974-12-24 | Department Of Health Education | Elution centrifuge-apparatus and method |
| US5024758A (en) * | 1988-12-20 | 1991-06-18 | The United States Of America As Represented By The Department Of Health And Human Services | Horizontal flow-through coil planet centrifuge with multilayer plural coils in eccentric synchronous rotation, suitable for counter-current chromatography |
| CN1405559A (en) * | 2002-10-25 | 2003-03-26 | 浙江大学 | Vertical high-speed counter-flow chromatograph |
| CN2670945Y (en) * | 2003-12-29 | 2005-01-12 | 杜琪珍 | Improved counter current cromatographic apparatus |
| CN2781368Y (en) * | 2005-03-24 | 2006-05-17 | 中国农业科学院茶叶研究所 | Large capacity counter-flow chromatograph dewind by mechanical device |
| CN206192959U (en) * | 2016-12-02 | 2017-05-24 | 山东省分析测试中心 | Novel but counter weight counter current chromatography separator column |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110960886A (en) * | 2019-11-11 | 2020-04-07 | 四川大学 | High-speed counter-current chromatographic separation column |
| CN112345677A (en) * | 2020-11-09 | 2021-02-09 | 四川大学 | Single-side self-unwinding high-speed counter-current chromatograph with double separation columns |
| CN115469047A (en) * | 2022-09-21 | 2022-12-13 | 四川大学华西医院 | Counter-current chromatographic separation column without adapter |
| CN115469047B (en) * | 2022-09-21 | 2023-08-15 | 四川大学华西医院 | Countercurrent chromatographic separation column without adapter |
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