CN108795784A - A kind of feed addictive and preparation method thereof, using and prepare the culture medium of feed addictive - Google Patents
A kind of feed addictive and preparation method thereof, using and prepare the culture medium of feed addictive Download PDFInfo
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- CN108795784A CN108795784A CN201810743173.0A CN201810743173A CN108795784A CN 108795784 A CN108795784 A CN 108795784A CN 201810743173 A CN201810743173 A CN 201810743173A CN 108795784 A CN108795784 A CN 108795784A
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- feed addictive
- culture medium
- culture
- trace element
- organic substance
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- 239000001963 growth medium Substances 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 97
- 238000006243 chemical reaction Methods 0.000 claims abstract description 50
- 229910052742 iron Inorganic materials 0.000 claims abstract description 49
- PMVSDNDAUGGCCE-TYYBGVCCSA-L Ferrous fumarate Chemical compound [Fe+2].[O-]C(=O)\C=C\C([O-])=O PMVSDNDAUGGCCE-TYYBGVCCSA-L 0.000 claims abstract description 48
- 239000011773 ferrous fumarate Substances 0.000 claims abstract description 39
- 235000002332 ferrous fumarate Nutrition 0.000 claims abstract description 39
- 229960000225 ferrous fumarate Drugs 0.000 claims abstract description 39
- 239000011573 trace mineral Substances 0.000 claims abstract description 37
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 37
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 29
- 230000036039 immunity Effects 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 48
- 238000000855 fermentation Methods 0.000 claims description 46
- 230000004151 fermentation Effects 0.000 claims description 46
- 230000009286 beneficial effect Effects 0.000 claims description 36
- 239000000126 substance Substances 0.000 claims description 33
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 27
- 239000000843 powder Substances 0.000 claims description 24
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 22
- 239000000654 additive Substances 0.000 claims description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 21
- 230000000996 additive effect Effects 0.000 claims description 21
- 239000008103 glucose Substances 0.000 claims description 21
- 238000004519 manufacturing process Methods 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 229920002472 Starch Polymers 0.000 claims description 20
- 239000008107 starch Substances 0.000 claims description 20
- 235000019698 starch Nutrition 0.000 claims description 20
- 238000011534 incubation Methods 0.000 claims description 15
- 235000013379 molasses Nutrition 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 12
- 239000004202 carbamide Substances 0.000 claims description 12
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 11
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 11
- 210000004369 blood Anatomy 0.000 claims description 10
- 239000008280 blood Substances 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000011218 seed culture Methods 0.000 claims description 9
- 230000003519 ventilatory effect Effects 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000011068 loading method Methods 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000002518 antifoaming agent Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000010899 nucleation Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 2
- 210000002216 heart Anatomy 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 10
- 239000002609 medium Substances 0.000 abstract description 10
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 239000006041 probiotic Substances 0.000 abstract description 5
- 235000018291 probiotics Nutrition 0.000 abstract description 5
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 208000007502 anemia Diseases 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000003394 haemopoietic effect Effects 0.000 abstract description 3
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 235000013406 prebiotics Nutrition 0.000 abstract description 2
- 241000235342 Saccharomycetes Species 0.000 description 20
- 238000012360 testing method Methods 0.000 description 19
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 18
- 108010088751 Albumins Proteins 0.000 description 7
- 102000009027 Albumins Human genes 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 206010012735 Diarrhoea Diseases 0.000 description 6
- 102000001554 Hemoglobins Human genes 0.000 description 6
- 108010054147 Hemoglobins Proteins 0.000 description 6
- 210000003743 erythrocyte Anatomy 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 239000011790 ferrous sulphate Substances 0.000 description 5
- 235000003891 ferrous sulphate Nutrition 0.000 description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 4
- 230000009102 absorption Effects 0.000 description 4
- 235000014590 basal diet Nutrition 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 241000282887 Suidae Species 0.000 description 3
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 239000003674 animal food additive Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000003862 health status Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002255 vaccination Methods 0.000 description 3
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003026 anti-oxygenic effect Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- -1 bromine cresols Chemical class 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000037308 hair color Effects 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 description 1
- 229960001008 heparin sodium Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- FWFGVMYFCODZRD-UHFFFAOYSA-N oxidanium;hydrogen sulfate Chemical compound O.OS(O)(=O)=O FWFGVMYFCODZRD-UHFFFAOYSA-N 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Animal Husbandry (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Birds (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to livestock-raising technical fields, and in particular to a kind of feed addictive and preparation method thereof, using and prepare the culture medium of feed addictive.The present invention can stimulate the growth and breeding of candida utili to act on the stronger absorption and conversion capability and ferrous fumarate of Organic Iron in the medium using candida utili, the feed addictive of preparation contains profitable probliotics and trace element, pass through the combination of the two, the prebiotic double action of the physiology and probiotics of trace element can be played, present invention optimizes probiotics fermention conditions, have achieved the purpose that organic iron content height, Organic Iron high conversion rate.The feed addictive of the present invention is nontoxic for animal safety, and the significant effect in terms of promoting piglet to grow, promoting hematopoietic function, prevent piglet anaemia can significantly improve immunity.
Description
Technical field
The present invention relates to livestock-raising technical fields, and in particular to a kind of feed addictive and preparation method thereof, using and
Prepare the culture medium of feed addictive.
Background technology
Trace elements iron is usually added in human body or animal body in the form of inorganic salts to enhance immune function, is reduced each
Kind disease, but the trace element of inorganic states is more toxic, the level being absorbed by organisms is very low, and the micro- energy of organic
Enough absorptions rapid by body, safety, and can in animal body the long period retain and accumulate.Use microorganism special in recent years
Be not beneficial bacterium as the trace element needed for carrier enriched animal with obtain organic form, it is at low cost and be rich in a variety of nutrition
Tunning attracted people's attention as feed addictive, saccharomycete have stronger absorption and conversion capability, can inhale
Conversion trace element synthesis self component is received, improves the bioavailability of trace element, Organic Iron such as ferrous fumarate can be used for
Hypoferric anemia is treated, food and feed additive is also largely used to.
China Patent Publication No. CN104472940A, publication date 2015.04.01 disclose a kind of improvement weanling pig fur
Mixed feed, Organic Iron such as PORPHYRIN IRON and ferrous fumarate are added in feed and promotes weanling pig blood flow, promotes blood
It is ruddy to increase the colour of skin for red eggs Leukocytopoiesis.
China Patent Publication No. CN103947840A, publication date 2014.07.30, forage protein can be saved by disclosing one kind
Lactic acid, citric acid, ferrous fumarate and carrier are hybridly prepared into feed by the feed addictive and its preparation method of matter dosage and application
Additive is added in livestock and poultry cultivation mixed feed and uses, on the basis of ensureing livestock and poultry preferably production and growth performance, effectively
Additive amount of the protide feedstuff in feed for livestock is reduced, protein resource is saved, saves formulation space, reduces formula
Cost.
China Patent Publication No. CN106721070A, publication date 2017.05.31 disclose a kind of mixed type piglet feed and
The preparation method of the feed, in mixed fodder add trace element is mainly added to ferrous fumarate, and add probiotic by
Lactobacillus plantarum, bacillus licheniformis, saccharomycete composition and other powders and particulate material, can improve the body machine of sucking pig
Can, make sucking pig smooth mistake from nursing period to weaning period, solve feed supplement hardly possible, wean difficult problem.
The prior art is disclosed to be directly appended to as chalybeate in feed ferrous fumarate using being, but directly
It takes ferrous fumarate and stimulation is generated to animal intestinal tract, and existing animal feed is mostly formulated by plurality of raw materials, cost
Height makes complexity, and candida utili can be grown it is not necessary that any growth factor is added in the medium, for Candida utilis
Yeast is used to prepare feed addictive with Organic Iron mixed fermentation acquisition tunning and has not been reported.
Invention content
For the above-mentioned prior art, the object of the present invention is to provide a kind of feed addictive and preparation method thereof, using and
Prepare the culture medium of feed addictive.
To achieve the above object, the technical solution adopted by the present invention is：
The culture medium of the organic substance containing trace element for beneficial bacterium conversion, the culture medium include containing micro
The organic substance of element, the organic substance are Organic Iron.
Preferably, the culture for the organic substance containing trace element of beneficial bacterium conversion as described in claim 1
Base, the Organic Iron are ferrous fumarate.
Preferably, the formula of the culture medium includes the component of following mixed mass concentration：10~20g/ of glucose
L, 5~10g/L of starch, 20~50g/L of urea, 10~20g/L of yeast powder, 0.15~0.20g/L of dipotassium hydrogen phosphate, seven water sulfuric acid
0.40~0.50g/L of magnesium, 0.5~1g/L of ferrous fumarate, and molasses additive amount 5~8% based on mass fraction.
Preferably, the formula of the culture medium includes the component of following mixed mass concentration：Glucose 20g/L, it forms sediment
Powder 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate 0.176g/L, epsom salt 0.456g/L, fumaric acid are sub-
Iron 0.8g/L, and molasses additive amount 8% based on mass fraction.
It includes that beneficial bacterium contains the organic of trace element in described for beneficial bacterium conversion that the present invention, which also protects a kind of,
The feed addictive of the tunning to ferment in the culture medium of substance, the beneficial bacterium are production Ruan's Candida, the feeding
Feed additives further include auxiliary material.
Preferably, the auxiliary material is starch.
The present invention also protects the preparation method of feed addictive, includes the following steps：
Step 1：Actication of culture
Candida utili solid culture is inoculated in YPD culture mediums activation culture, and (YPD culture mediums form：Peptone
10.0g, yeast extract powder 5.0g, glucose 20.0g, distilled water 1000mL), 28 DEG C of 30~40h of culture are so activated three times,
Bacterium count plate carries out (with reference to national standard GB 4789.15-2016 moulds and yeasts counts), and bacterium solution is diluted to 1 × 108
~1 × 109CFU/mL is as seed liquor.
Step 2：Seed culture
The candida utili seed liquor obtained in step 1 is accessed into seeding tank with 2% inoculum concentration, loading amount coefficient is
60%~70% volume ratio, the formula of seed culture medium include the component of following mixed mass concentration：Glucose 20g/L,
Peptone 20g/L, yeast extract 10g/L, fermentation condition are 28 DEG C, rotating speed 200rpm, pH value 6-6.5 of fermentation temperature, ventilatory capacity
1.2：1, incubation time for 24 hours, obtains candida utili fermentation seed liquid.
Step 3：Commodity fermentation tank culture
The candida utili fermentation seed liquid obtained in step 2 is accessed into commodity fermentation tank, dress with 5% inoculum concentration
Coefficient of discharge is 60%~70% volume ratio, and molasses first pass through >=80 DEG C of heating, time >=2 hour separation slagging-off, then through 125-
135 DEG C of high-temperature short-time sterilizations, time are 9 seconds, are pumped into commodity fermentation tank, and fermentation condition is 25 DEG C, rotating speed 200rpm of temperature, pH value
6-6.5 ventilatory capacity 1.2：1, incubation time 36h are automatically added to antalkali NaOH when ph decreases, automatic when foam is excessive
Antifoaming agent Tween 80 is added, obtains candida utili zymotic fluid.
Step 4：Microorganism collection
By the candida utili zymotic fluid obtained in step 3 be pumped into rotating speed be l500r/min continuous centrifugal machine into
Row centrifugation, abandons supernatant, obtains wet thallus.
Step 5：The preparation of viable bacteria powder
The wet thallus that collection step four obtains, with dried starch with mass ratio 1：5 mixing, low temperature drying, pulverizer crush, mistake
40 mesh sieve, and viable count >=1 × 10 of Mixed Microbes are adjusted with dried starch8CFU/g。
The application of feed addictive is also claimed in the present invention, and the feed addictive is exempted from for piglet growth promotion, raising
Epidemic disease power improves blood physiological and biochemical indices.
The beneficial effects of the present invention are：
(1) it is used for the culture medium of the organic substance containing trace element of beneficial bacterium conversion, Organic Iron is added, using beneficial
Bacterium prepares that organic iron content is high, feed addictive of Organic Iron high conversion rate for the stronger absorption and conversion capability of Organic Iron,
Organic Iron is more easy to be absorbed by animal body simultaneously.
(2) utilize candida utili in the culture medium hair of the organic substance containing trace element converted for beneficial bacterium
Feed addictive prepared by ferment, iron-holder 0.623mg/g, Organic Iron conversion ratio is 61.3%, and converts remaining Organic Iron
It is environmentally safe.
(3) ferrous fumarate can stimulate the growth and breeding of candida utili, the combination of the two, energy in the medium
The prebiotic double action of the enough physiology and probiotics for playing trace element.
(4) this feed addictive is nontoxic for animal safety, is imitated in terms of promoting piglet growth, in terms of reduction incidence
Fruit is apparent, is promoting hematopoietic function, is preventing the quantitative aspects effect of piglet anaemia, raising immunocyte and immunoglobulin aobvious
It writes, immunity can be significantly improved.
Description of the drawings
Fig. 1 is Organic Iron Different adding amount to production Ruan's Candida viable count and Organic Iron conversion ratio influence curve.
Fig. 2 produces Ruan's Candida fermentation seed liquid different vaccination amount to production Ruan's Candida viable count and Organic Iron conversion ratio
Influence curve.
Fig. 3 is that different cultivation temperatures influence song to production Ruan's Candida viable count and Organic Iron conversion ratio in commodity fermentation tank
Line.
Fig. 4 is that different incubation times influence song to production Ruan's Candida viable count and Organic Iron conversion ratio in commodity fermentation tank
Line.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with embodiment, should be intended that, this
Invention is not limited to following specific implementation modes, and based on the embodiments of the present invention, those of ordinary skill in the art are not doing
The all other embodiment obtained under the premise of going out creative work, shall fall within the protection scope of the present invention.
One, the culture medium of feed addictive and the organic substance containing trace element for beneficial bacterium conversion
Feed addictive includes candida utili (BNCC140331 comes from Bei Na biological studies institute) for beneficial bacterium
The tunning that fermentation obtains in the culture medium of the organic substance containing trace element of conversion, further includes supplementary product starch, wherein
The culture medium of the organic substance containing trace element for beneficial bacterium conversion includes such as rich horse of the Organic Iron containing trace element
It is sour ferrous, by adding Organic Iron in the medium, using candida utili for the stronger sorption enhanced energy of Organic Iron
Power, ferrous fumarate can stimulate the growth and breeding of candida utili in the medium, improve the conversion ratio of Organic Iron, and convert
Remaining Organic Iron is environmentally safe, also can be by good absorption in feeding to animal body.
The formula for being wherein used for the culture medium of the organic substance containing trace element of beneficial bacterium conversion includes following mixing
The component of mass concentration afterwards：10~20g/L of glucose, 5~10g/L of starch, 20~50g/L of urea, 10~20g/ of yeast powder
L, 0.15~0.20g/L of dipotassium hydrogen phosphate, 0.40~0.50g/L of epsom salt, 0.5~1g/L of ferrous fumarate, and with matter
The molasses additive amount 5~8% of score meter is measured, specific embodiment is：
Embodiment 1：The culture medium of the organic substance containing trace element for beneficial bacterium conversion, by following mixed
The component of mass concentration is made：Glucose 20g/L, starch 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate
0.176g/L, epsom salt 0.456g/L, ferrous fumarate 0.5g/L, and molasses additive amount 8% based on mass fraction.
Embodiment 2：The culture medium of the organic substance containing trace element for beneficial bacterium conversion, by following mixed
The component of mass concentration is made：Glucose 20g/L, starch 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate
0.176g/L, epsom salt 0.456g/L, ferrous fumarate 1.0g/L, and molasses additive amount 8% based on mass fraction.
Embodiment 3：The culture medium of the organic substance containing trace element for beneficial bacterium conversion, by following mixed
The component of mass concentration is made：Glucose 20g/L, starch 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate
0.176g/L, epsom salt 0.456g/L, ferrous fumarate 0.8g/L, and molasses additive amount 8% based on mass fraction.
Embodiment 4：The culture medium of the organic substance containing trace element for beneficial bacterium conversion, by following mixed
The component of mass concentration is made：Glucose 10g/L, starch 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate
0.176g/L, epsom salt 0.456g/L, ferrous fumarate 0.8g/L, and molasses additive amount 8% based on mass fraction.
Embodiment 5：The culture medium of the organic substance containing trace element for beneficial bacterium conversion, by following mixed
The component of mass concentration is made：Glucose 20g/L, starch 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate are
0.156g/L, epsom salt 0.496g/L, ferrous fumarate 0.8g/L, and molasses additive amount 8% based on mass fraction.
Described in above example 1-5 for beneficial bacterium conversion containing trace element organic substance culture medium it is each
The mass concentration of component refers both to the mass concentration after each component mixes.
Two, the preparation of feed addictive：
Embodiment 6
1, material
1.1 strain：Candida utili, BNCC140331 come from Bei Na biological studies institute.
1.2 reagent：Ferrous fumarate, Tween 80 are that analysis is pure.
1.3 culture medium prescription
1.3.1 activation medium：YPD culture mediums (are made of each component of following quality：Peptone 10.0g, yeast leach
Powder 5.0g, glucose 20.0g, distilled water 1000mL).
1.3.2 seed culture medium：It is made of the component of following mass concentration：Glucose 20g/L, peptone 20g/L, yeast
Soak powder 10g/L.
1.3.3 it is used for the culture medium of the organic substance containing trace element of beneficial bacterium conversion：By following mixed matter
The component of amount concentration is made：Glucose 20g/L, starch 10g/L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate
0.176g/L, epsom salt 0.456g/L, ferrous fumarate 0.8g/L, and molasses additive amount 8% based on mass fraction.
2, preparation method
Step 1：Actication of culture
Candida utili solid culture is inoculated in YPD culture medium activation cultures, and 28 DEG C of culture 30h so activate three
Secondary, bacterium count plate carries out (with reference to national standard GB 4789.15-2016 moulds and yeasts counts), bacterium solution is diluted to 1 ×
108CFU/mL is as seed liquor.
Step 2：Seed culture
The candida utili seed liquor obtained in step 1 is accessed into seeding tank with 2% inoculum concentration, loading amount coefficient is
60% volume ratio, the formula of seed culture medium：Glucose 20g/L, peptone 20g/L, yeast extract 10g/L, fermentation condition are
28 DEG C, rotating speed 200rpm of fermentation temperature, pH value 6, ventilatory capacity 1.2：1, incubation time for 24 hours, obtains candida utili fermentation kind
Sub- liquid.
Step 3：Commodity fermentation tank culture
The candida utili fermentation seed liquid obtained in step 2 is accessed into commodity fermentation tank, dress with 5% inoculum concentration
Coefficient of discharge is 60% volume ratio, is sent out in the culture medium of the organic substance containing trace element converted for beneficial bacterium
Ferment, molasses first pass through 80 DEG C of heating, and time 2 h separation slagging-off, then through 125 DEG C of high-temperature short-time sterilizations, the time is 9 seconds, pump
Enter commodity fermentation tank, fermentation condition is 25 DEG C, rotating speed 200rpm of temperature, pH value 6, ventilatory capacity 1.2：1, incubation time 36h work as pH
It is automatically added to antalkali NaOH when reduction, antifoaming agent Tween 80 is automatically added to when foam is excessive, obtains candida utili
Zymotic fluid.
Step 4：Microorganism collection
By the candida utili zymotic fluid obtained in step 3 be pumped into rotating speed be l500r/min continuous centrifugal machine into
Row centrifugation, abandons supernatant, obtains wet thallus.
Step 5：The preparation of viable bacteria powder
The wet thallus that collection step four obtains, with dried starch with mass ratio 1：5 mixing, low temperature drying, pulverizer crush, mistake
40 mesh sieve, and 1 × 109CFU/g of viable count of Mixed Microbes is adjusted with dried starch.
3, Organic Iron conversion ratio measures：With reference to the assay method (GB/T of iron, copper, manganese, zinc, magnesium in national standard-feed
13885-92), using aas determination ferro element.It is 0.623mg/ to take iron-holder in 1.0g viable bacteria powder determination samples
G separately takes 1.0g zinc-rich probiotic 1:After 5 dilutions, 3 000r/min centrifuge 8min, measure the iron content in sediment, calculate
It is 61.3% to go out Organic Iron conversion ratio.
Organic Iron conversion ratio=(the sediment iron content/total iron-holder of sample) × 100%
4, safety examination
Using 5 days chick, 18 gram/day of feed addictive is admixed in feed, continuous raising 30 days, with control group ratio
Compared with chick no abnormality seen and death.Toxicology inspection result shows the heart, liver,spleen,kidney and control group no significant difference, test group
Animal postmortem and pathological examination show no obvious abnormalities change.Feed addictive does not show animal any toxic effect, belongs to
A kind of safe Application of Direct-fed Microbials.
Three, the optimization of commodity ferment tank condition
Embodiment 7
1, material：
1.1 strain：Candida utili, BNCC140331 come from Bei Na biological studies institute.
1.2 reagent：Ferrous fumarate, Tween 80 are that analysis is pure.
1.3 culture medium prescription
1.3.1 activation medium：YPD culture mediums (are made of each component of following quality：Peptone 10.0g, yeast leach
Powder 5.0g, glucose 20.0g, distilled water 1000mL).
1.3.2 seed culture medium：It is made of the component of following mass concentration：Glucose 20g/L, peptone 20g/L, yeast
Soak powder 10g/L.
1.3.3 fermentation medium：It is made of the component of following mass concentration：Glucose 20g/L, starch 10g/L, urea
50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate 0.176g/L, epsom salt 0.456g/L, and molasses based on mass fraction
Additive amount 8%.
2, method：Compare different ferrous fumarate additive amounts, inoculum concentration, cultivation temperature, incubation time for tunning
The influence of middle production Ruan's Candida count plate and Organic Iron conversion ratio
2.1 ferrous fumarate additive amount contrast tests
The following ferrous fumarate of mass concentration is added in the fermentation medium in commodity fermentation tank：0.4g/L,0.6g/
L, 0.8g/L, 1.0g/L, 1.2g/L obtain the culture medium of the organic substance containing trace element converted for beneficial bacterium, will be through
The production Ruan's Candida fermentation seed liquid for crossing seed culture medium culture is inoculated according to 5% amount and contains for what beneficial bacterium converted
In the culture medium of the organic substance of trace element, 25 DEG C, rotating speed 200rpm of fermentation temperature, pH value 6, ventilatory capacity 1.2：1, when culture
Between 36h.Production Ruan's Candida count plate is carried out after culture, while measuring Organic Iron conversion ratio.
2.2 different vaccination amount contrast tests
Production Ruan's Candida fermentation seed liquid is inoculated according to 1%, 2%, 5%, 7.5%, 10% inoculum concentration respectively
(addition of wherein ferrous fumarate is in the culture medium of the organic substance containing trace element for beneficial bacterium conversion
0.8g/L), 25 DEG C of fermentation temperature, rotating speed 200rpm, pH value 6, ventilatory capacity 1.2：1, incubation time 36h are carried out after culture
Ruan's Candida count plate is produced, while measuring Organic Iron conversion ratio.
2.3 cultivation temperature contrast tests
By produce Ruan's Candida fermentation seed liquid according to 5% inoculum concentration be inoculated in for beneficial bacterium convert contain it is micro
In the culture medium of the organic substance of element (wherein the addition of ferrous fumarate is 0.8g/L), rotating speed 200rpm, pH value 6 is led to
Tolerance 1.2：1, respectively with 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 37 DEG C of incubation time 36h, production Ruan's vacation silk ferment is carried out after culture
Female count plate, while measuring Organic Iron conversion ratio.
2.4 incubation time contrast tests
By produce Ruan's Candida fermentation seed liquid according to 5% inoculum concentration be inoculated in for beneficial bacterium convert contain it is micro
In the culture medium of the organic substance of element (wherein the addition of ferrous fumarate is 0.8g/L), rotating speed 200rpm, pH value 6 is led to
Tolerance 1.2：1, respectively with 25 DEG C culture for 24 hours, 30h, 36h, 48h, 72h, carried out after culture production Ruan's Candida viable bacteria meter
Number, while measuring Organic Iron conversion ratio.
3, result
3.1, ferrous fumarate additive amount is for producing Ruan's Candida count plate and Organic Iron conversion ratio in tunning
Influence
As shown in Fig. 1, when ferrous fumarate additive amount is 0.8g/L, Ruan Candida viable bacteria is produced in feed addictive
It is counted as highest 10.3 × 108The conversion ratio of CFU/g, Organic Iron are up to 61.3%, it is thus determined that for beneficial bacterium conversion
The addition of ferrous fumarate is 0.8g/L in the culture medium of organic substance containing trace element.
3.2, production Ruan's Candida different vaccination amount is for producing Ruan's Candida count plate and organic in feed addictive
The influence of iron conversion ratio
As shown in Fig. 2, turn for beneficial bacterium when production Ruan's Candida fermentation seed liquid is inoculated according to 5% inoculum concentration
When in the culture medium for the organic substance containing trace element changed, production Ruan's Candida count plate is highest in feed addictive
9.99×108The conversion ratio of CFU/g, Organic Iron are highest 60.91%, it is thus determined that production Ruan's Candida fermentation seed liquid connects
Kind measures the amount according to 5%.
3.3, influence of the cultivation temperature for production Ruan's Candida count plate and Organic Iron conversion ratio in feed addictive
As shown in Fig. 3, when the cultivation temperature in commodity fermentation tank is 25 DEG C, Ruan's Candida is produced in feed addictive
Count plate is highest 10.08 × 108The conversion ratio of CFU/g, Organic Iron are highest 61.82%, it is thus determined that cultivation temperature is
25℃。
3.4, influence of the incubation time for production Ruan's Candida count plate and Organic Iron conversion ratio in feed addictive
As shown in Fig. 4, when incubation time is 36h, production Ruan's Candida count plate is highest in feed addictive
10.33×108The conversion ratio of CFU/g, Organic Iron are highest 62.21%, it is thus determined that incubation time is 36h.
4, brief summary
By filtering out its suitable fermentation item in the Optimum Experiment of commodity fermentation cylinder for fermentation condition to feed addictive
Part is that the amount of addition ferrous fumarate in the fermentation medium is 0.8g/L, and the inoculum concentration of production Ruan's Candida fermentation seed liquid is
5%, cultivation temperature is 25 DEG C, incubation time 36h.
Four, the application of feed addictive of the present invention
Embodiment 8：Feed addictive clinic growth promotion, disease resisting effect verification
1, experimental design
28 age in days wean sodium selenite 150 of experiment selection Nanyang, henan pig farm, is randomly divided into 5 groups, respectively blank pair
According to group, ferrous sulfate control group (ferrous sulfate is added according to 200g/ tons of basal diet routine additive amounts), ferrous fumarate control
Group (ferrous fumarate is added according to 100g/ tons of basal diet routine additive amounts), saccharomycete control group is (according to 200g/ tons of bases
Daily ration adds pure saccharomycete), feed addictive addition group (according to 200g/ ton basal diets addition feed addictives).Every group sets 3
A repetition each repeats 10 pigs, and basal diet formula is shown in Table 1, feeds utilized to be prepared by feed workshop is unified, feeds utilized base
Plinth raw material is identical.According to conventinal breeding method management, experimental period 30d.Before and after on-test, per component, also known as weight, calculating are flat
Equal weight and rate of body weight gain.
The conventional Diet Formula of table 1 and trophic component
2, Testing index and method
2.1 growth-promoting effect
It is also known as heavy per component before and after on-test, calculate average weight and rate of body weight gain.
2.2 health status and incidence
It observes and records daily.By observing test group piglet and the spirit of control group piglet, feed intake, hair color, skin
Gloss, coloured light brightness, excrement shape and color and luster, excrement is smooth, sophistication, whether there is or not indigested feed granules, the sick pigs of morbidity
Head number, to judge the healthcare function of feed addictive.
3, results and discussion
3.1 gaining effect
As shown in Table 2, the average day total augment weight of feed addictive group every is all remarkably higher than other each groups, compares blank control group
Average daily gain improve 16.99%；Average daily gain than ferrous sulfate group improves 10.03%；Compare ferrous fumarate
The average daily gain of group improves 7.93%；Average daily gain than saccharomycete group improves 4.87%；Test result shows to raise
Feed additives group gaining effect is more apparent than individually addition ferrous sulfate, ferrous fumarate, saccharomycete.
Gaining effect of 2 feed addictive of table to weanling pig
Note：Same column shoulder mark indicates difference not significantly (P > 0.05) without letter or the identical person of letter；The different small letter of shoulder mark
Matrix shows significant difference (P < 0.05), and following table is same.
3.2 health status and incidence
3 health status of table and incidence
Test result is shown in Table 3, and feed addictive group passes through 30 days feeding feed stuff additives, and pig spirit, appetite have larger
Improve, especially the smooth exquisiteness of excrement, there are no and do not digest feed, no off-balancesheet parasite phenomenon.In experimental period, feed addictive
Diarrhea averagely occurs altogether for group experimental group piglet 5 times, diarrhea number incidence 12.5%；It is reduced than blank control group Incidence of Diarrhea
77.71%；68.35% is reduced than ferrous sulfate group Incidence of Diarrhea；It is reduced than ferrous fumarate group Incidence of Diarrhea
58.33%；16.67% is reduced than saccharomycete group Incidence of Diarrhea.Test result shows feed addictive disease resisting effect than single
Solely addition ferrous sulfate, ferrous fumarate, saccharomycete are apparent.
Embodiment 9：Application of the feed addictive in terms of improving immunity, improving blood physiological and biochemical indices
1, material
Kit and instrument：Total protein (TP) kit, albumin (ALB) kit, total cholesterol (TC) kit, entirely
Automatic blood cell analyzer, automatic clinical chemistry analyzer, it is above to be provided by First People's Hospital of Henan Province.
2, detection method and index
Piglet venous blood, 6 pigs of every group of extraction are acquired before experiment and after experiment, every pig acquires two pipes.
One pipe is not added with anticoagulation to detach serum, a pipe plus heparin sodium anti-freezing, while First People's Hospital of Henan Province being sent to detect physiology
And biochemical indicator, detection reagent, instrument, method are provided by First People's Hospital of Henan Province.
(1) Blood Physiological Indexes：Measure red blood cell (RBC), leucocyte (WBC), hemoglobin (HGB), packed cell volume
(HCT) etc..
(2) blood parameters：Total protein (TPRO) uses biuret method, albumin albumin (ALB) to use bromine cresols
Green colorimetric method, globulin are the difference of total protein and albumin, and urea nitrogen (SUN) uses urease method.
3, Blood Physiological Indexes results and discussion
4 are the results are shown in Table, leucocyte (WBC) testing result shows that feed addictive group leukocyte count magnitude compares blank respectively
Control group, ferrous sulfate group, ferrous fumarate group, saccharomycete group have more 20.23% (p ﹤ 0.05), 12.08% (p ﹤ 0.05)；
7.41% (p ﹤ 0.05)；2.67% (p>0.05) feed addictive, is added in daily ration in terms of improving piglet immunological cell quantity
It is more with obvious effects than ferrous sulfate, ferrous fumarate, saccharomycete addition group.
Red blood cell (RBC) testing result show feed addictive group erythrocyte number average value respectively than blank control group,
Ferrous sulfate group, ferrous fumarate group, saccharomycete group have more 10.60% (p ﹤ 0.05), 4.94% (p ﹤ 0.05), 1.34% (p ﹤
0.05), 9.88% (p ﹤ 0.05)；Hemoglobin (HGB) testing result shows feed addictive group amount of hemoglobin average value
Respectively 12.47% (p ﹤ 0.05), 7.29% (p are had more than blank control group, ferrous sulfate group, ferrous fumarate group, saccharomycete group
﹤ 0.05), 5.77% (p ﹤ 0.05), 9.71% (p ﹤ 0.05).Packed cell volume (HCT) testing result shows feed addictive group
Packed cell volume (HCT) numerical value has more than blank control group, ferrous sulfate group, ferrous fumarate group, saccharomycete group respectively
14.16% (p ﹤ 0.05), 6.99% (p ﹤ 0.05), 4.08% (p>0.05), 9.81% (p ﹤ 0.05).The above result shows that day
Feed addictive significant effect in terms of promoting hematopoietic function, preventing piglet anaemia is added in grain.
4 Blood Physiological Indexes of table
| Group | WBC/(109/L) | RBC/(1012/L) | HGB/(g/L) | HCT/% |
| Blank control group | 21.03±1.57a | 6.13±2.30a | 120.71±13.41a | 40.2±4.8a |
| Ferrous sulfate group | 22.01±1.98b | 6.46±4.76b | 127.01±10.86b | 42.9±11.43a |
| Ferrous fumarate group | 22.97±1.92b | 6.69±4.09b | 128.35±10.13b | 44.1±11.02b |
| Saccharomycete group | 24.03±1.82c | 6.18±8.12a | 123.55±14.12b | 41.8±0.56a |
| Feed addictive group | 24.67±1.45c | 6.78±7.58c | 135.76±19.05c | 45.9±2.7b |
4, blood parameters
Influence of 4.1 feed addictives to piglet haemocyanin
Feed addictive the results are shown in Table 5 to the influence of the haemocyanin of weanling pig, and total protein (TPRO) testing result shows
Feed addictive group total protein quantity is more than blank control group, ferrous sulfate group, ferrous fumarate group, saccharomycete group difference respectively
Go out 5.13% (p ﹤ 0.05), 1.49% (p ﹥ 0.05), 1.02% (p ﹥ 0.05), 0.73% (p ﹥ 0.05)；Albumin (ALB) detects
The result shows that feed addictive group is more than blank control group, ferrous sulfate group, ferrous fumarate group, saccharomycete group difference respectively
Go out 15.77% (p ﹤ 0.05), 10.86% (p ﹤ 0.05), 5.70% (p ﹥ 0.05), 5.62% (p ﹥ 0.05)；As a result feed is prompted
Additive can significantly improve immunocyte quantity；Globulin testing result show feed addictive group respectively than blank control group,
Ferrous sulfate group, ferrous fumarate group, saccharomycete group have more respectively 17.18% (p ﹤ 0.05), 14.73% (p ﹤ 0.05),
5.91% (p ﹥ 0.05), 3.94% (p ﹥ 0.05) as a result prompt feed addictive that can significantly improve the quantity of immunoglobulin, carry
High immunity.
Influence of 5 feed addictive of table to weanling pig haemocyanin
Influence of 4.2 feed addictives for piglet serum antioxygenic property and urea nitrogen
Influence of 6 feed addictive of table to weanling pig serum antioxygenic property and urea nitrogen
| Index | SOD(U/mL) | GSH-Px(U/gprot) | MDA(nmol/mL) | SUN(mmol/L) |
| Blank control group | 60.78±9.51a | 583.76±29.31 | 9.29±1.67a | 1.74±2.72 |
| Ferrous sulfate group | 61.01±17.11a | 689.75±31.31 | 8.53±4.71a | 1.67±6.23 |
| Saccharomycete group | 65.29±19.04b | 692.15±24.54 | 7.86±5.13b | 1.61±5.06 |
| Feed addictive group | 66.89±10.89b | 698.71±32.35 | 7.27±2.09b | 1.62±4.03 |
Test result is shown in Table 6, the results showed that, feed addictive group serum superoxide dismutases (SOD) content compares respectively
Blank control group, ferrous sulfate group, saccharomycete group have more 10.05% (p ﹤ 0.05), 9.64% (p ﹤ 0.05), 2.45% (p respectively
﹥ 0.05)；The result shows that addition feed addictive can remove internal excessive free radical, the immunity of pig is improved.Feed adds
Agent group Serum glutathione peroxidase (GSH-Px) content is with other groups without significant difference.
Feed addictive group Serum MDA (MDA) content is respectively than blank control group, ferrous sulfate group, saccharomycete component
21.74% (p ﹤ 0.05), 14.77% (p ﹤ 0.05), 7.50% (p ﹥ 0.05) are not had more；The result shows that feed addictive group can be with
Cytotoxicity caused by free radical is reduced, immunity is improved.Feed addictive group urea nitrogen (SUN) content has certain reduction to make
With the not notable (P of experimental group group difference>0.05), illustrate that adding feed addictive does not influence the renal function of pig.
Feed addictive in the present invention can improve the growth performance of weanling pig and significantly improve immunocompetence, reduce
Incidence, to prove validity of the feed addictive for pig healthy growth.
Claims (8)
1. the culture medium of the organic substance containing trace element for beneficial bacterium conversion, it is characterised in that：The culture medium packet
The organic substance containing trace element is included, the organic substance is Organic Iron.
2. the culture medium for the organic substance containing trace element of beneficial bacterium conversion as described in claim 1, feature
It is：The Organic Iron is ferrous fumarate.
3. the culture medium for the organic substance containing trace element of beneficial bacterium conversion as claimed in claim 2, feature
It is：The formula of the culture medium includes the component of following mixed mass concentration：10~20g/L of glucose, starch 5~
10g/L, 20~50g/L of urea, 10~20g/L of yeast powder, 0.15~0.20g/L of dipotassium hydrogen phosphate, epsom salt 0.40~
0.50g/L, 0.5~1g/L of ferrous fumarate, and molasses additive amount 5~8% based on mass fraction.
4. such as the culture medium of the organic substance containing trace element according to claim 3 for beneficial bacterium conversion,
It is characterized in that：The formula of the culture medium includes the component of following mixed mass concentration：Glucose 20g/L, starch 10g/
L, urea 50g/L, yeast powder 20g/L, dipotassium hydrogen phosphate 0.176g/L, epsom salt 0.456g/L, ferrous fumarate 0.8g/
L, and molasses additive amount 8% based on mass fraction.
5. a kind of includes that beneficial bacterium is containing micro- organic substance for beneficial bacterium conversion as described in claim 1
Culture medium in the feed addictive of tunning that ferments, it is characterised in that：The beneficial bacterium is production Ruan's Candida,
The feed addictive further includes auxiliary material.
6. feed addictive as claimed in claim 5, it is characterised in that：The auxiliary material is starch.
7. the preparation method of feed addictive as claimed in claim 5, it is characterised in that：Include the following steps：
(1) step 1：Actication of culture
Candida utili solid culture is inoculated in YPD culture mediums activation culture, and (YPD culture mediums form：Peptone 10.0g,
Yeast extract powder 5.0g, glucose 20.0g, distilled water 1000mL), 28 DEG C of 30~40h of culture are so activated three times, bacteria in viable
Bacterium counts to carry out (with reference to national standard GB 4789.15-2016 moulds and yeasts counts), and bacterium solution is diluted to 1 × 108~1 ×
109CFU/mL is as seed liquor.
(2) step 2：Seed culture
The candida utili seed liquor obtained in step 1 is accessed into seeding tank with 2% inoculum concentration, loading amount coefficient is 60%
~70% volume ratio, the formula of seed culture medium include the component of following mixed mass concentration：Glucose 20g/L, albumen
Peptone 20g/L, yeast extract 10g/L, fermentation condition are 28 DEG C, rotating speed 200rpm, pH value 6-6.5 of fermentation temperature, ventilatory capacity 1.2：
1, incubation time for 24 hours, obtains candida utili fermentation seed liquid.
(3) step 3：Commodity fermentation tank culture
The candida utili fermentation seed liquid obtained in step 2 is accessed into commodity fermentation tank, loading amount system with 5% inoculum concentration
Number is 60%~70% volume ratio, and molasses first pass through >=80 DEG C of heating, time >=2 hour separation slagging-off, then through 125-135 DEG C
High-temperature short-time sterilization, time are 9 seconds, are pumped into commodity fermentation tank, and fermentation condition is 25 DEG C, rotating speed 200rpm, pH value 6- of temperature
6.5, ventilatory capacity 1.2：1, incubation time 36h are automatically added to antalkali NaOH, add automatically when foam is excessive when ph decreases
Enter antifoaming agent Tween 80, obtains candida utili zymotic fluid.
(4) step 4：Microorganism collection
By the candida utili zymotic fluid obtained in step 3 be pumped into rotating speed be l500r/min continuous centrifugal machine carry out from
The heart abandons supernatant, obtains wet thallus.
(5) step 5：The preparation of viable bacteria powder
The wet thallus that collection step four obtains, with dried starch with mass ratio 1：5 mixing, low temperature drying, pulverizer crush, and cross 40 mesh
Sieve adjusts viable count >=1 × 10 of Mixed Microbes with dried starch9CFU/g。
8. the application of the feed addictive as described in claim 5-7 is any, it is characterised in that：The feed addictive is for son
Pig growth promotion improves immunity, improves blood physiological and biochemical indices.
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| JPH09248179A (en) * | 1996-03-13 | 1997-09-22 | Kohjin Co Ltd | Production of iron-containing yeast |
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