CN108795748A - A kind of device and method for the sunflower disk functional activity peptide preparing drop high lithemia - Google Patents
A kind of device and method for the sunflower disk functional activity peptide preparing drop high lithemia Download PDFInfo
- Publication number
- CN108795748A CN108795748A CN201810472188.8A CN201810472188A CN108795748A CN 108795748 A CN108795748 A CN 108795748A CN 201810472188 A CN201810472188 A CN 201810472188A CN 108795748 A CN108795748 A CN 108795748A
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- China
- Prior art keywords
- duplex
- filter
- functional activity
- sunflower disk
- activity peptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The present invention relates to a kind of device and method for the sunflower disk functional activity peptide preparing drop high lithemia, the equipment includes enzymatic vessel, duplex polymeric membrane filter, duplex high speed tubular type centrifugal chiller, duplex macromolecular filtering device, purpose ceramic-film filter, filtrate tanks are already expired and do not filter liquid storage tank, enzymatic vessel, duplex polymeric membrane filter, duplex high speed tubular type centrifugal chiller, duplex macromolecular filtering device and purpose ceramic-film filter are sequentially connected in series, the filtering outlet of purpose ceramic-film filter and non-filtering outlet, which are separately connected, to be already expired filtrate tanks and does not filter liquid storage tank, liquid storage tank is not filtered to connect with enzymatic vessel；Circulating pump is equipped between enzymatic vessel and duplex polymeric membrane filter, high speed tubular type centrifugal chiller includes three high speed tube centrifuges being arranged in parallel.Extensive, serialization production can be achieved in the present invention, is suitable for extraction, enzymolysis and the separation of large biological molecule, can be used for reducing the large-scale production of the functional micromolecule polypeptide of uric acid.
Description
Technical field
The present invention relates to separation of biopolymer equipment technical field more particularly to a kind of sunflower disks preparing drop high lithemia
The device and method of functional activity peptide.
Background technology
Phytoprotein enzymolysis is prepared in the technique of active peptides, and influencing most significant 3 factors of Degree of Enzymatic Hydrolysis is：It is enzyme
Amount, enzymolysis time and hydrolysis temperature.But in industrial scale production, to realize extensive, serialization enzymolysis with it is suitable
The separation of molecular weight product, balance with to adjust be more crucial influence link.
Invention content
The present invention is intended to provide preparing the device and method of the sunflower disk functional activity peptide of drop high lithemia, it can be achieved that big rule
The production of mould, serialization.
In order to achieve the above objectives, the technical solution adopted by the present invention is as follows：
A kind of equipment for the sunflower disk functional activity peptide preparing drop high lithemia, including the filtering of enzymatic vessel, duplex polymeric membrane
Duplex high speed tubular type centrifugal chiller, duplex macromolecular filtering device, purpose ceramic-film filter, filtrate tanks and non-filtered fluid are already expired in device
Storage tank, the enzymatic vessel, duplex polymeric membrane filter, duplex high speed tubular type centrifugal chiller, duplex macromolecular filtering device and pottery
Porcelain membrane filter is sequentially connected in series, and the filtering outlet of the purpose ceramic-film filter is connect with filtrate tanks are already expired, purpose ceramic-film filter
Non- filtering outlet with do not filter liquid storage tank and connect, the liquid storage tank that do not filter is connect with enzymatic vessel；Enzymatic vessel and duplex high score
Circulating pump A is equipped between sub- membrane filter.
Further, surge tank and circulating pump are equipped between duplex high speed tubular type centrifugal chiller and duplex macromolecular filtering device
B, circulating pump B are located between surge tank and duplex macromolecular filtering device.
Further, purpose ceramic-film filter is connected with high pressure recoil gas tank.
Preferably, each high speed tubular type centrifugal chiller includes three high speed tube centrifuges.
Wherein, purpose ceramic-film filter is microfiltration ceramic membrane filter.
Further, the aperture of the ceramic membrane of purpose ceramic-film filter is 100nm.
Further, the mesh number of polymeric membrane filter is 1000.
Further, the mesh number of macromolecular filtering device is 2000.
Wherein, further include extractor, the extractor connects fluid reservoir, and the fluid reservoir connects enzymatic vessel.
Further, further include nano grinder, the nano grinder is connect with extractor.
A method of sunflower disk activity peptide extract being prepared, it is the sunflower disk function using above-mentioned preparation drop high lithemia
The equipment extraction of active peptide.
Compared with prior art, the invention has the advantages that：
Extensive, serialization production can be achieved in the present invention, is suitable for extraction, enzymolysis and the separation of large biological molecule, can
For reducing the large-scale production of the functional micromolecule polypeptide of uric acid.
Description of the drawings
Fig. 1 is the structural schematic diagram of equipment in the present invention；
In figure：1- enzymatic vessels, 2- duplex polymeric membranes filter, 3- duplex high speed tubular types centrifugal chiller, 4- duplex high scores
Sub- filter, 5- purpose ceramic-film filters, 6- are already expired filtrate tanks, 7- and do not filter liquid storage tank, 8- circulating pump A, 9- extractor, 10-
Nano grinder, 11- surge tanks, 12- high pressures recoil gas tank, 13- circulating pump B, 14- fluid reservoirs.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with attached drawing, to the present invention into
Row is further described.
As shown in Figure 1, the equipment of the sunflower disk functional activity peptide disclosed by the invention for preparing drop high lithemia, including enzymatic vessel
1, duplex polymeric membrane filter 2, duplex high speed tubular type centrifugal chiller 3, duplex macromolecular filtering device 4, purpose ceramic-film filter 5,
Filtrate tanks 6 are already expired and do not filter liquid storage tank 7, enzymatic vessel 1, duplex polymeric membrane filter 2, duplex high speed tubular type centrifugal chiller
3, duplex macromolecular filtering device 4 and purpose ceramic-film filter 5 are sequentially connected in series, the filtering outlet of purpose ceramic-film filter 5 and filtered fluid
Storage tank 6 connects, and the non-filtering outlet of purpose ceramic-film filter 5 is connect with liquid storage tank 7 is not filtered, does not filter liquid storage tank 7 and enzymatic vessel 1
Connection；Circulating pump A8 is equipped between enzymatic vessel 1 and duplex polymeric membrane filter 2.Each high speed tubular type centrifugal chiller includes extremely
Few two high speed tube centrifuges being arranged in parallel, preferably, each high speed tubular type centrifugal chiller includes three high speed tubular types
Centrifuge.
Surge tank 11 and circulating pump B13 are equipped between duplex high speed tubular type centrifugal chiller 3 and duplex macromolecular filtering device 4,
Circulating pump B13 is located between surge tank 11 and duplex macromolecular filtering device 4.Purpose ceramic-film filter 5 is connected with high pressure recoil gas tank
12。
The mesh number of macromolecular filtering device is more than the mesh number of duplex polymeric membrane filter.Polymeric membrane filters in the present embodiment
The mesh number of device is 1000, and the mesh number of macromolecular filtering device is 2000.Wherein, purpose ceramic-film filter 5 is microfiltration ceramic membrane filter；
Preferably, the aperture of the ceramic membrane of purpose ceramic-film filter 5 is 100nm.
A method of sunflower disk activity peptide extract being prepared, it is the sunflower disk function using above-mentioned preparation drop high lithemia
The equipment extraction of active peptide.
In use, raw material enters extractor 9 after the crushing of nano grinder 10, extractor 9 connects fluid reservoir 14, fluid reservoir
14 connect enzymatic vessels 1, and the decomposed solution in enzymatic vessel 1 drives through circulating pump A8, is first replaced with duplex polymeric membrane filter 2
Filter, using two groups it is in parallel, can the duplex high speed tubular type centrifugal chiller 3 of alternate run be filtered, filtered solution injects surge tank
11.Under the driving of circulating pump B13, the filtered fluid in surge tank 11 first passes through can alternate run, two polymeric membrane mistakes in parallel
Filter, filtered solution are separated into two parts liquid with purpose ceramic-film filter 5 again, wherein the filtered solution across ceramic membrane filter plate is
100nm or less micromolecule polypeptide mixed liquors enter and filtrate tanks 6 are already expired, spare；And be not passed through ceramic membrane filter plate is
The mixed liquor of 100nm or more polypeptides is directly entered and does not filter liquid storage tank 7, which can be slowly injected into enzymatic vessel 1, is formed
Enzymolysis-separation cycle.So cycle, until it becomes 100nm molecular products below.It is 1.5KDa that preparative separation, which obtains molecular weight,
The active peptides of~15KDa, and the molecular weight of active peptides is concentrated mainly on 10KDa ranges.Concentrate is by allotment, then uses mouth
It takes liquid bottle placer and is filled into oral liquid bottle；Or solid state powder is used and is packed, for further preparing capsule, tablets and other formulations
With.
Extensive, serialization production can be achieved in the present invention, is suitable for extraction, enzymolysis and the separation of large biological molecule, can
For reducing the large-scale production of the functional micromolecule polypeptide of uric acid.
Certainly, the present invention can also have other numerous embodiments, without deviating from the spirit and substance of the present invention,
Those skilled in the art can make various corresponding change and deformations, but these corresponding changes and change according to the present invention
Shape should all belong to the protection domain of appended claims of the invention.
Claims (10)
1. a kind of equipment for the sunflower disk functional activity peptide preparing drop high lithemia, it is characterised in that:Including enzymatic vessel (1), duplex
Polymeric membrane filter (2), duplex high speed tubular type centrifugal chiller (3), duplex macromolecular filtering device (4), purpose ceramic-film filter
(5), filtrate tanks (6) are already expired and do not filter liquid storage tank (7), the enzymatic vessel (1), duplex polymeric membrane filter (2), duplex
High speed tubular type centrifugal chiller (3), duplex macromolecular filtering device (4) and purpose ceramic-film filter (5) are sequentially connected in series, the ceramic membrane mistake
The filtering outlet of filter (5) is connect with filtrate tanks (6) are already expired, non-filtering outlet and the non-filtered fluid of purpose ceramic-film filter (5)
Storage tank (7) connects, and the liquid storage tank (7) that do not filter is connect with enzymatic vessel (1)；Enzymatic vessel (1) and duplex polymeric membrane filter
(2) circulating pump A (8) is equipped between.
2. the equipment of the sunflower disk functional activity peptide according to claim 1 for preparing drop high lithemia, it is characterised in that：Duplex
Surge tank (11) and circulating pump B (13), cycle are equipped between high speed tubular type centrifugal chiller (3) and duplex macromolecular filtering device (4)
Pump B (13) is located between surge tank (11) and duplex macromolecular filtering device (4).
3. the equipment of the sunflower disk functional activity peptide according to claim 1 or 2 for preparing drop high lithemia, it is characterised in that：
Purpose ceramic-film filter (5) is connected with high pressure recoil gas tank (12).
4. the equipment of the sunflower disk functional activity peptide according to claim 1 for preparing drop high lithemia, it is characterised in that：Each
High speed tubular type centrifugal chiller includes three high speed tube centrifuges.
5. the equipment of the sunflower disk functional activity peptide according to claim 1 for preparing drop high lithemia, it is characterised in that：Ceramics
Membrane filter (5) is microfiltration ceramic membrane filter.
6. the equipment of the sunflower disk functional activity peptide according to claim 5 for preparing drop high lithemia, it is characterised in that：Ceramics
The aperture of the ceramic membrane of membrane filter (5) is 100nm.
7. the equipment of the sunflower disk functional activity peptide according to claim 1 for preparing drop high lithemia, it is characterised in that：High score
The mesh number of sub- membrane filter is 1000.
8. the equipment of the sunflower disk functional activity peptide according to claim 1 or claim 7 for preparing drop high lithemia, it is characterised in that：
The mesh number of macromolecular filtering device is 2000.
9. the equipment of the sunflower disk functional activity peptide according to claim 1 for preparing drop high lithemia, it is characterised in that：Also wrap
Extractor (9) and nano grinder (10) are included, the nano grinder (10) connect with extractor (9), and the extractor (9) is even
Connect fluid reservoir (14), fluid reservoir (14) the connection enzymatic vessel (1).
10. a kind of method preparing sunflower disk activity peptide extract, it is characterised in that：It is using any in claim 1-9
What the equipment described in item claim was extracted.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810453229 | 2018-05-11 | ||
| CN2018104532299 | 2018-05-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108795748A true CN108795748A (en) | 2018-11-13 |
| CN108795748B CN108795748B (en) | 2020-08-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810472188.8A Active CN108795748B (en) | 2018-05-11 | 2018-05-16 | Equipment and method for preparing high-uric-acid-reducing sunflower disc functional active peptide |
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| CN (1) | CN108795748B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624495A (en) * | 2018-05-11 | 2018-10-09 | 四川师范大学 | A method of preparing the sunflower disk functional activity peptide of drop high lithemia |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087639A (en) * | 2014-07-08 | 2014-10-08 | 吴庆林 | Method for extracting micromolecular active substances from sunflower calathide |
| CN105125610A (en) * | 2015-09-16 | 2015-12-09 | 吉林大学 | Application of sunflower disc hydrolysis compound powder in preparing of medicine for curing or relieving high uric acid gout |
| CN105753935A (en) * | 2016-05-06 | 2016-07-13 | 洮南市金塔生物科技有限公司 | Small-molecule peptides of sunflower head as well as preparation method and medical application of small-molecule peptide |
| CN108624495A (en) * | 2018-05-11 | 2018-10-09 | 四川师范大学 | A method of preparing the sunflower disk functional activity peptide of drop high lithemia |
| CN208667747U (en) * | 2018-05-11 | 2019-03-29 | 成都市益康堂药业有限公司 | A kind of enzymatic hydrolysis separation interlocking equipment being used to prepare drop high lithemia nano functional peptide |
-
2018
- 2018-05-16 CN CN201810472188.8A patent/CN108795748B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087639A (en) * | 2014-07-08 | 2014-10-08 | 吴庆林 | Method for extracting micromolecular active substances from sunflower calathide |
| CN105125610A (en) * | 2015-09-16 | 2015-12-09 | 吉林大学 | Application of sunflower disc hydrolysis compound powder in preparing of medicine for curing or relieving high uric acid gout |
| CN105753935A (en) * | 2016-05-06 | 2016-07-13 | 洮南市金塔生物科技有限公司 | Small-molecule peptides of sunflower head as well as preparation method and medical application of small-molecule peptide |
| CN108624495A (en) * | 2018-05-11 | 2018-10-09 | 四川师范大学 | A method of preparing the sunflower disk functional activity peptide of drop high lithemia |
| CN208667747U (en) * | 2018-05-11 | 2019-03-29 | 成都市益康堂药业有限公司 | A kind of enzymatic hydrolysis separation interlocking equipment being used to prepare drop high lithemia nano functional peptide |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624495A (en) * | 2018-05-11 | 2018-10-09 | 四川师范大学 | A method of preparing the sunflower disk functional activity peptide of drop high lithemia |
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|---|---|
| CN108795748B (en) | 2020-08-25 |
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