CN217092934U - Extraction and concentration device for iturin - Google Patents
Extraction and concentration device for iturin Download PDFInfo
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- CN217092934U CN217092934U CN202220192141.8U CN202220192141U CN217092934U CN 217092934 U CN217092934 U CN 217092934U CN 202220192141 U CN202220192141 U CN 202220192141U CN 217092934 U CN217092934 U CN 217092934U
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Abstract
The utility model discloses an extraction enrichment facility of iturin, include: the ultrafiltration membrane filter is used for carrying out ultrafiltration treatment on the iturin extracting solution to remove thalli and proteins; the activated carbon decoloring kettle is connected to the permeation side of the ultrafiltration membrane filter and is used for decoloring the ultrafiltration penetrating fluid; the nanofiltration membrane filter is connected to the activated carbon decoloring kettle and is used for carrying out nanofiltration and desalination treatment on the decolored liquid; the drying device is connected to the interception side of the nanofiltration membrane filter and is used for drying the nanofiltration concentrated solution to obtain iturin; wherein, the interception side connecting port of the nanofiltration membrane filter is simultaneously connected with the dialysate adding port of the ultrafiltration membrane filter. The device can be used for continuous production, has low energy consumption in the purification process, and can effectively separate the iturin from the fermentation liquor to obtain the high-purity iturin.
Description
Technical Field
The utility model relates to an extraction and concentration device of iturin belongs to the fermentation product and draws technical field.
Background
The iturin is a cyclic lipopeptide antibiotic with broad-spectrum antifungal activity generated by bacillus through level metabolism, the molecular structure of the iturin is composed of a peptide chain consisting of seven amino acid residues and a beta-amino fatty acid side chain (chain lengths are different from C14-C17), the iturin can be widely used for preventing and treating fungal diseases of various crops, has the advantages of lasting bacteriostasis, good stability, difficulty in generating drug resistance, safety to environment and the like, and is a novel biological pesticide with great research and development values and application prospects.
At present, demand for iturin is increasing, but in the prior art, iturin fermentation liquor is generally obtained by adding a flocculating agent into iturin fermentation liquor for flocculation and sedimentation, and then performing centrifugation, ultrafiltration and concentration, but the iturin obtained by the method has lower purity and lower recovery rate, and the quality of a final iturin product is influenced.
Disclosure of Invention
The utility model discloses the technical problem that will solve is: provided is a production apparatus capable of continuously operating and extracting iturin from a fermentation liquid, and having the advantages of high recovery rate and product extraction purity.
An extraction and concentration device of iturin, comprising:
the ultrafiltration membrane filter is used for carrying out ultrafiltration treatment on the iturin extracting solution to remove thalli and proteins;
the activated carbon decoloring kettle is connected to the permeation side of the ultrafiltration membrane filter and is used for decoloring the ultrafiltration penetrating fluid;
the nanofiltration membrane filter is connected to the activated carbon decoloring kettle and is used for carrying out nanofiltration and desalination treatment on the decolored liquid;
the drying device is connected to the interception side of the nanofiltration membrane filter and is used for drying the nanofiltration concentrated solution to obtain iturin;
wherein, the interception side connecting port of the nanofiltration membrane filter is simultaneously connected with the dialysate adding port of the ultrafiltration membrane filter.
The ultrafiltration membrane in the ultrafiltration membrane filter is a ceramic ultrafiltration membrane.
The molecular weight cut-off of the ceramic ultrafiltration membrane is 100 KD-150 KD.
The ceramic ultrafiltration membrane is of a single-tube or multi-channel structure.
The granularity of the activated carbon loaded in the activated carbon decoloring kettle is 30-50 meshes.
The nanofiltration membrane in the nanofiltration membrane filter is a ceramic nanofiltration membrane.
The cut-off molecular weight of the ceramic nanofiltration membrane is 200-800 Da.
The drying device is a spray drying device.
Further comprising: and the iturin extracting solution storage tank is connected to the feed inlet of the ultrafiltration membrane filter and is used for storing iturin extracting solution.
Further comprising: and the product tank is connected to the discharge port of the drying device and used for storing products.
Advantageous effects
The utility model discloses a device is including the milipore filter that connects gradually, activated carbon decoloration cauldron, receive filter of filter membrane filter and drying device, can serialization production, purification process energy consumption low through above-mentioned device, can separate iturin and zymotic fluid effectively, obtains high-purity iturin.
Drawings
FIG. 1 is a flow chart of the present invention
FIG. 2 is the device diagram of the present invention, wherein 1-iturin extract storage tank, 2-ultrafiltration membrane filter, 3-activated carbon decolorizing kettle, 4-nanofiltration membrane filter, 5-drying device, 6-product tank, 8-interception side connector, and 7-dialysate inlet.
Detailed Description
The utility model discloses an extraction and concentration device of iturin, extraction and concentration device is including iturin extract storage tank 1, milipore filter 2, active carbon decoloration cauldron 3, receive filter 4, drying device 5 and product jar 6 that connect gradually.
The ultrafiltration membrane filter 2 is used for carrying out ultrafiltration on the iturin fermentation liquor to remove impurities such as thalli, suspended matters, macromolecular proteins and the like so as to obtain ultrafiltration clear liquid; the ultrafiltration membrane in the ultrafiltration membrane filter used herein is preferably a ceramic membrane, and should have a tubular or multi-channel structure with a diameter of 52mm, a length of 1.5m, 61 channels and a channel diameter of 4mm, and a single-membrane tube filtration area of 1.29m 2 . The molecular weight cut-off of the ceramic membrane ultrafiltration membrane is 100 KD-150 KD, the system operation pressure is 0.4-0.7 Mpa, the membrane surface flow rate is 4m/s, and the concentration multiple is more than 10 times.
An activated carbon decoloring kettle 3 for decoloring the ultrafiltration clear liquid obtained by the ultrafiltration membrane filter and removing colored substances to obtain an activated carbon permeate; in the operation filtration, the operating temperature of the decoloring kettle is controlled to be 35-50 ℃, and the decoloring operation time is controlled to be 0.5-2 h. The activated carbon decoloring kettle 3 is used for adsorbing and decoloring activated carbon powder with the granularity of 30-50 meshes to remove colored substances.
A nanofiltration membrane filter 4, which is used for concentrating and desalting the activated carbon permeate obtained by the activated carbon decoloring kettle 3, adding pure water for dialysis after the activated carbon permeate is concentrated to a certain multiple, and concentrating again to remove salt, partial water and small molecular amino acid impurities, so as to obtain high-concentration iturin concentrated solution; the adopted nanofiltration membrane filter element is a ceramic nanofiltration membrane, the aperture is 1-2 nm, the cut-off molecular weight is about 200-800 Da, the system operating pressure is 1.5-2.5 Mpa, and the concentration multiple is more than 6 times. The permeation side of the nanofiltration membrane filter is connected with the ultrafiltration membrane filter so as to recycle nanofiltration membrane permeation liquid as dialysis water of the ultrafiltration membrane filter, thereby improving the yield of iturin.
The drying device 5 is used for carrying out spray drying on the high-concentration iturin concentrated solution obtained by the nanofiltration membrane filter so as to obtain iturin powder;
example 1
Step 1: a certain amount of fermentation liquor containing iturin enters an ultrafiltration membrane filter 2 through a feed pump and a circulating pump, and impurities such as thalli, suspended matters, macromolecular proteins and the like are removed, so that ultrafiltration clear liquid is obtained. Wherein the ultrafiltration membrane filter adopts a ceramic ultrafiltration membrane, the diameter of the ultrafiltration membrane filter is 52mm, the length of the ultrafiltration membrane filter is 1.5m, 61 channels are arranged, the diameter of each channel is 4mm, and the filtering area of a single membrane tube is 1.29m 2 . The molecular weight cut-off of the ceramic membrane ultrafiltration membrane is 100 KD. In the ultrafiltration membrane filter, the system operation pressure is 0.4Mpa, the membrane surface flow rate is 4m/s, and the concentration multiple is 12 times.
Step 2: and (3) introducing the ultrafiltration clear liquid obtained in the step (1) into an activated carbon decoloring kettle 3 with the average particle size of 30 meshes, and treating at 45 ℃ for 1h to remove pigment impurities so as to obtain activated carbon permeate.
And 3, step 3: and (3) concentrating the activated carbon permeate obtained in the step (2) to a certain multiple through a nanofiltration membrane filter 4, adding pure water for dialysis, and concentrating again to remove salt and partial water, micromolecular amino acid and other impurities, so that a high-concentration iturin concentrated solution and a nanofiltration membrane permeate are obtained, and the nanofiltration membrane permeate is reused as dialysis water of the ultrafiltration membrane filter, so that the yield of iturin is improved. The intercepted molecular weight of a special nanofiltration membrane filter element adopted by the nanofiltration membrane filter is about 800Da, the system operating pressure is 2Mpa, and the concentration multiple is 8 times.
And 4, step 4: and (3) passing the high-concentration iturin concentrated solution obtained in the step (3) through a drying device 5 to obtain iturin powder.
Example 2
Step 1: and (3) feeding a certain amount of fermentation liquor containing iturin into an ultrafiltration membrane filter 2 through a feed pump and a circulating pump to remove impurities such as thalli, suspended matters, macromolecular proteins and the like, thereby obtaining ultrafiltration clear liquid. Wherein the ultrafiltration membrane filter adopts a ceramic ultrafiltration membrane, the diameter of the ultrafiltration membrane filter is 52mm, the length of the ultrafiltration membrane filter is 1.5m, 61 channels are arranged, the diameter of each channel is 4mm, and the filtering area of a single membrane tube is 1.29m 2 . The molecular weight cut-off of the ceramic membrane ultrafiltration membrane is 120 KD. In the ultrafiltration membrane filter, the system operation pressure is 0.5Mpa, the membrane surface flow rate is 4m/s, and the concentration multiple is 18 times.
Step 2: and (3) introducing the ultrafiltration clear liquid obtained in the step (1) into an activated carbon decoloring kettle 3 with the average particle size of 35 meshes, and treating at 40 ℃ for 1h to remove pigment impurities so as to obtain activated carbon permeate.
And 3, step 3: and (3) concentrating the activated carbon permeate obtained in the step (2) to a certain multiple by a nanofiltration membrane filter 4, adding pure water for dialysis, and concentrating again to remove salt, part of water, micromolecular amino acid and other impurities to obtain high-concentration iturin concentrate and nanofiltration membrane permeate, wherein the nanofiltration membrane permeate is used as dialysis water of the ultrafiltration membrane filter for recycling, so that the yield of iturin is improved. The special nanofiltration membrane filter element adopted by the nanofiltration membrane filter has the trapped molecular weight of about 1000Da, the system operating pressure is 1.8Mpa, and the concentration multiple is 8 times.
And 4, step 4: and (3) passing the high-concentration iturin concentrated solution obtained in the step (3) through a drying device 5 to obtain iturin powder.
Comparative example 1
Compared with example 1, the differences are: the ultrafiltration was performed using a commercially available organic polysulfone ultrafiltration membrane.
Comparative example 2
Compared with example 1, the difference is that: the ultrafiltration was performed using a commercially available polyamide nanofiltration membrane.
Comparative example 3
Compared with example 1, the difference is that: the nanofiltration membrane permeate is not recycled as the dialysis water of the ultrafiltration membrane filter.
TABLE 1 quality and purity of iturin powder obtained in different examples and comparative examples
| Obtained iturin powder mass (g) | Purity (%) of the obtained iturin powder | |
| Example 1 | 2.41 | 93.6 |
| Example 2 | 2.38 | 93.5 |
| Comparative example 1 | 2.33 | 84.6 |
| Comparative example 2 | 2.45 | 87.3 |
| Comparative example 3 | 1.95 | 93.5 |
Can see out from the table, through the utility model discloses a method can obtain high-purity iturin powder, and its purity can reach more than 93%, and does not carry out the scheme of retrieval and utilization as the dialysis water of milipore filter for the nanofiltration membrane permeate liquid, and the rate of recovery of product promotes more than 20%.
Claims (10)
1. An extraction and concentration device of iturin, which is characterized by comprising:
an ultrafiltration membrane filter (2) for carrying out ultrafiltration treatment on the iturin extracting solution to remove thalli and protein;
the activated carbon decoloring kettle (3) is connected to the permeation side of the ultrafiltration membrane filter (2) and is used for decoloring the ultrafiltration penetrating fluid;
a nanofiltration membrane filter (4) connected to the activated carbon decoloring kettle (3) and used for carrying out nanofiltration and desalination treatment on the decolored liquid;
the drying device (5) is connected to the interception side of the nanofiltration membrane filter (4) and is used for drying the nanofiltration concentrated solution to obtain iturin;
wherein, a interception side connecting port (8) of the nanofiltration membrane filter (4) is connected with a dialysate adding port (7) of the ultrafiltration membrane filter (2).
2. The apparatus for extracting and concentrating iturin as claimed in claim 1, wherein the ultrafiltration membrane in the ultrafiltration membrane filter (2) is a ceramic ultrafiltration membrane.
3. The apparatus for extracting and concentrating iturin as claimed in claim 2, wherein the ceramic ultrafiltration membrane has a molecular weight cut-off of 100-150 KD.
4. The apparatus for extracting and concentrating iturin as claimed in claim 3, wherein the ceramic ultrafiltration membrane is of single-tube or multi-channel structure.
5. The apparatus for extracting and concentrating iturin as claimed in claim 1, wherein the activated carbon decoloring tank (3) is charged with activated carbon of 30-50 mesh size.
6. The apparatus for extracting and concentrating iturin as claimed in claim 1, wherein the nanofiltration membrane in the nanofiltration membrane filter (4) is a ceramic nanofiltration membrane.
7. The apparatus for extracting and concentrating iturin as claimed in claim 6, wherein the ceramic nanofiltration membrane has a molecular weight cut-off of 200-800 Da.
8. The apparatus for extracting and concentrating iturin as claimed in claim 1, wherein said drying apparatus (5) is a spray drying apparatus.
9. The apparatus for extracting and concentrating iturin as claimed in claim 1, further comprising: and the iturin extracting solution storage tank (1) is connected with a feed inlet of the ultrafiltration membrane filter (2) and is used for storing iturin extracting solution.
10. The apparatus for extracting and concentrating iturin as claimed in claim 1, further comprising: and the product tank (6) is connected with the discharge hole of the drying device (5) and is used for storing products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220192141.8U CN217092934U (en) | 2022-01-25 | 2022-01-25 | Extraction and concentration device for iturin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220192141.8U CN217092934U (en) | 2022-01-25 | 2022-01-25 | Extraction and concentration device for iturin |
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| CN217092934U true CN217092934U (en) | 2022-08-02 |
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| CN202220192141.8U Active CN217092934U (en) | 2022-01-25 | 2022-01-25 | Extraction and concentration device for iturin |
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