CN108785156B

CN108785156B - New application of cerasus humilis juice

Info

Publication number: CN108785156B
Application number: CN201710306230.4A
Authority: CN
Inventors: 李卫东
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-05-04
Filing date: 2017-05-04
Publication date: 2021-07-23
Anticipated expiration: 2037-05-04
Also published as: CN108785156A

Abstract

The invention relates to application of cerasus humilis juice in preparing an external cosmetic or pharmaceutical preparation with a whitening effect. The study of the invention shows that the cerasus humilis juice can reduce the melanin value of the skin and has obvious whitening effect.

Description

New application of cerasus humilis juice

Technical Field

The invention relates to a new application of cerasus humilis juice, in particular to a new application of cerasus humilis juice in preparing external cosmetics or pharmaceutical preparations with whitening effect.

Background

Prunus humilis Bunge (calcium fruit)Cerasus humilisBelongs to small shrubs of cerasus of Rosaceae, is unique to China, does not have the species abroad, and the wild species is mainly distributed in inner Mongolia, Liaoning, Jilin, Heilongjiang, Shanxi, Hebei, Beijing, Tianjin, Henan, Shandong, Shaanxi and other areas. Cerasus humilis fruit contains various mineral elements beneficial to human body, especially calcium element with higher content than that of common fruit, so it is also called "calcium fruit". The cerasus humilis bunge (Prunus humilis bunge) has extremely high economic value, very wide application, edible pulp, seeds which are the medicinal material of the prunus humilis bunge, roots which can also be used as the medicine, flowers which are similar to cherry blossom are not good enough, and the development and utilization prospect is very wide.

The cerasus humilis (Prunus humilis Bunge) fruits are reddish, glittering and lovely, are oblate, round with jujube and pointed and different in shape, thick in pulp, moderate in sour and sweet, unique in taste, rich in nutrition, and superior to walnut fruits such as cherries, plums, apricots, peaches and the like, and contain multiple nutritional types and high content. The content of vitamin A, vitamin B2, vitamin B12, vitamin C and vitamin E, mineral elements such as potassium, calcium, magnesium, phosphorus, iron, manganese and zinc is rich, the content of the mineral elements is several times to dozens of times higher than that of the mineral elements, the mineral elements contain 18 amino acids including lysine, and the sugar content of mature fruits is 14% -19%. The content of calcium and iron in each hundred grams of fresh fruits is 7-10 times and 6-10 times of that of apples respectively, and the content of calcium and iron in each hundred grams of fresh fruits reaches 60-90 milligrams and 1.5-2.5 milligrams.

The whitening active substance is a natural or artificial compound which can reduce skin chroma or reduce pigmentation so as to achieve the purpose of whitening the skin. The tyrosinase activity, melanin transportation and other links in the melanin synthesis process are easily influenced by the outside, so that a foundation is provided for the action link of the whitening active substance. The main action targets of the daily whitening active substances are inhibition of tyrosinase activity, influence on melanin metabolism and transportation and other links. The approaches to skin whitening and whitening products can be summarized as follows: (1) melanocyte selective toxicity: hydroquinone; (2) inhibition of tyrosinase activity: kojic acid, arbutin, vitamin C and derivatives thereof, and the like; (3) inhibition of dopamine tautomerase activity: licoflavone; (4) inhibition of DHICA oxidase activity: kojic acid and its derivatives, vitamin C and its derivatives; (5) inhibition of melanosome transport processes: a nicotinamide; (6) promoting absorption and metabolism of pigment: fruit acid, Chinese herbal medicines for promoting blood circulation and removing blood stasis and the like; (7) oxygen radical removal: vitamin C and its derivatives, SOD, vitamin E and its derivatives, beta-carotene; (8) modulation of melanocyte and tyrosinase activity: endothelin antagonists, MSH antagonists; (9) preventing tanning by ultraviolet rays: various sunscreens.

Disclosure of Invention

The invention aims to provide application of cerasus humilis juice in preparing cosmetics or pharmaceutical preparations with whitening effect for external use.

The cosmetic or pharmaceutical preparation of the present invention includes a mask, a patch, a lotion, an ointment, a cream, etc.

The cerasus humilis juice is prepared from cerasus humilis fruits by adopting a conventional squeezing process.

Preferably, the soluble solid content of the cerasus humilis juice is 10% -21%; further preferably, the soluble solid content of the cerasus humilis juice is 13-17%.

The invention further provides a cerasus humilis facial mask which takes cerasus humilis juice as a raw material component; wherein, the content of the cerasus humilis juice is 5-10% (weight percentage); preferably, the content of the cerasus humilis juice is 5% -8%; further preferably, the concentration of the cerasus humilis juice is 5% -6%; most preferably, the concentration of the cerasus humilis juice is 5%.

The soluble solid content of the cerasus humilis juice is 10% -21%; further preferably, the soluble solid content of the cerasus humilis juice is 13-17%.

Further, the mask also comprises the following auxiliary material components: humectants such as glycerol, 1, 3-butanediol, propylene glycol; thickeners, such as xanthan gum; whitening agents, such as dipotassium glycyrrhizinate; lubricants such as allantoin, water-soluble jojoba oil.

Preferably, the mask comprises the following raw and auxiliary materials: 4-10% of cerasus humilis fruit juice, 2-6% of 1, 3-butanediol, 0.2-0.6% of xanthan gum, 0.05-0.2% of dipotassium glycyrrhizinate, 1-4% of glycerol, 0.05-0.2% of allantoin, 0.1-0.5% of water-soluble jojoba oil, 0.3-1% of propylene glycol and the balance of water.

Further preferably, the raw and auxiliary materials of the mask comprise: 4-8% of cerasus humilis fruit juice, 3-5% of 1, 3-butanediol, 0.3-0.5% of xanthan gum, 0.08-0.15% of dipotassium glycyrrhizinate, 1-3% of glycerol, 0.08-0.15% of allantoin, 0.2-0.4% of water-soluble jojoba oil, 0.4-0.8% of propylene glycol and the balance of water.

Most preferably, the raw and auxiliary materials of the mask comprise: 5% of cerasus humilis juice, 4% of 1, 3-butanediol, 0.4% of xanthan gum, 0.1% of dipotassium glycyrrhizinate, 2% of glycerol, 0.1% of allantoin, 0.3% of water-soluble jojoba oil, 0.5% of propylene glycol and the balance of water.

The raw material component is a component with a whitening effect, namely the cerasus humilis juice; the adjuvant components are components with thickening, lubricating, and moisturizing effects, such as 1, 3-butanediol, xanthan gum, dipotassium glycyrrhizinate, etc.

The research of the invention shows that under the conditions of high temperature, severe cold and ultraviolet irradiation, the cerasus humilis juice has stable property and is not easy to be influenced by external conditions to generate the phenomena of precipitation, discoloration, oxidation, deterioration and the like, so the cerasus humilis juice can be added into the preparation and production of cosmetics. Human body experiments show that the cerasus humilis juice can reduce the melanin value, and the whitening effect is obvious.

Drawings

FIG. 1 Effect of a mask containing 2% cerasus humilis juice on the melanin value of the skin

FIG. 2 the effect of a mask containing 5% cerasus humilis juice on the melanin value of the skin

Detailed Description

EXAMPLE 1 preparation of Prunus humilis juice

Step a, selecting materials and juicing: crushing fully mature cerasus humilis fruits by using a crusher, and juicing by using a juicer; step b, fine filtration: filtering the juice obtained in the step a by using a plurality of layers of gauze; step c, clarification: freezing the finely filtered juice at 0 deg.C for a week, clarifying, and filtering to obtain clarified juice; step e, sterilizing and cooling: and c, sealing the clear juice obtained in the step c, putting the sealed clear juice into a sterilization pot, sterilizing the clear juice for 30 seconds at the temperature of 80 ℃, taking the clear juice out, and cooling the clear juice to be below 38 ℃.

Example 2 measurement of physicochemical Properties of Prunus humilis Bunge juice

The pH, soluble solids content, conductivity and stability under high temperature, cold and uv conditions were measured for the prunus humilis juice prepared in example 1.

1 method of experiment

1.1 measurement of pH, soluble solid content, and conductivity of Prunus humilis Bunge juice

Determination of pH: measured with a pH meter

And (3) determination of soluble solid content: measuring soluble solid content of Prunus humilis Bunge juice at 25 deg.C with electronic soluble solid content meter

Measurement of conductivity: measuring the conductivity of Prunus humilis Bunge juice at 25 deg.C with electronic conductivity meter

1.2 Prunus humilis juice stability test

The stability of the cerasus humilis juice under the conditions of high temperature, severe cold and ultraviolet rays is measured, and whether the property of the cerasus humilis juice changes or not is measured by the color change. The color change of the cerasus humilis juice is inspected by adopting a method of absorbance change, and then the stability of the cerasus humilis juice is evaluated. The change of absorbance is measured by using 500 nm.

1.2.1 high temperature conditions: and (3) respectively filling 10 g of cerasus humilis fruit juice samples into 5 test tubes, putting the test tubes into a 45 ℃ oven, taking out one test tube every 24 hours, storing the test tubes in a dark and cool place, and measuring the change condition of the absorbance after all the test tubes are taken out for 6 hours. Repeat 2 times.

1.2.2 Severe Cold conditions: and (3) respectively filling 10 g of cerasus humilis fruit juice samples into 5 test tubes, putting the test tubes into a refrigerator at the temperature of-5 ℃, taking out one test tube every 24 hours, storing the test tubes in a dark and cool place, and measuring the change condition of the absorbance after all the test tubes are taken out for 6 hours. Repeat 2 times.

1.2.3 ultraviolet conditions: putting 10 g cerasus humilis fruit juice sample into a quartz culture dish, irradiating for 0.5 h, 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 3.5 h and 4 h under a 20 w ultraviolet lamp at a distance of 30 cm, putting the irradiated sample into a test tube, storing in dark and cool places, taking out the sample, standing for 6 h, and determining the change condition of absorbance. Repeat 2 times. The choice of placing the sample in a quartz petri dish for irradiation is because a common glass petri dish is opaque to uv light, but quartz petri dishes are transparent to uv light.

2 results of the experiment

2.1 pH, solid content, conductivity of Prunus humilis Bunge juice

The pH, soluble solids content, conductivity measured by each instrument were as follows:

the pH of the Prunus humilis Bunge juice at 18 deg.C is 3.56.

The soluble solid content was 11.3%.

The conductivity was 2620 us/cm.

2.2 stability of Prunus humilis fruit juice

2.2.1 stability at high temperature conditions: the cerasus humilis juice was observed by naked eye without significant color change, and the measured absorbance change was as shown in table 1.

TABLE 1 change in absorbance under high temperature conditions

Conditions of the experiment	Repetition of 1	Repetition 2	Mean value of	Growth rate (% compared with the original)
					As received	1.514	1.513	1.514	0
Oven at 45 deg.C for 1 day	1.516	1.514	1.515	0.10
					Oven at 45 deg.C for 2 days	1.530	1.528	1.529	1.02
Oven at 45 deg.C for 3 days	1.540	1.543	1.542	1.85
					Oven at 45 deg.C for 4 days	1.546	1.546	1.546	2.15
Oven at 45 deg.C for 5 days	1.525	1.520	1.523	0.59

Table 1 shows that the change range and change rate of the absorbance value of the cerasus humilis juice in the oven 5 d at 45 ℃ are small, so the cerasus humilis juice has stable property at the high temperature of 45 ℃.

2.2.2 stability under severe Cold conditions: the cerasus humilis juice was observed by naked eye without significant color change, and the measured absorbance change is shown in table 2.

TABLE 2 Absorbance Change under Severe Cold conditions

Conditions of the experiment	Repetition of 1	Repetition 2	Mean value of	Growth rate (% compared with the original)
					As received	1.514	1.513	1.514	0
1 day at-5 deg.C	1.486	1.503	1.495	-1.26
					2 days at-5 deg.C	1.518	1.518	1.518	0.30
3 days at-5 DEG C	1.521	1.529	1.525	0.76
					4 days at-5 DEG C	1.537	1.536	1.537	1.52
5 days at-5 DEG C	1.535	1.528	1.532	1.19

Table 2 shows that the change range and the change rate of the absorbance value of the cerasus humilis juice are small under the severe cold condition of-5 ℃, so that the cerasus humilis juice has stable property under the severe cold condition of-5 ℃.

2.2.3 stability under UV irradiation: the observed cerasus humilis juice has no obvious color change, and the measured absorbance change is shown in table 3.

TABLE 3 change in absorbance under UV

Conditions of the experiment	Repetition of 1	Repetition 2	Mean value of	Growth rate (% compared with the original)
					As received	1.493	1.500	1.497	0
Ultraviolet ray exposure for 0.5 h	1.497	1.501	1.499	0.17
					Ultraviolet ray for 1 h	1.502	1.509	1.506	0.60
Ultraviolet ray for 1.5 h	1.520	1.511	1.516	1.27
					Ultraviolet ray for 2 h	1.527	1.551	1.539	2.84
Ultraviolet ray for 2.5 h	1.515	1.528	1.522	1.67
					Ultraviolet ray for 3 h	1.514	1.533	1.524	1.63
Ultraviolet ray exposure for 3.5 h	1.525	1.506	1.516	0.66
					Ultraviolet ray for 4 h	1.521	1.528	1.525	0.59

Table 3 shows that the change range and change rate of the absorbance values of the cerasus humilis juice are small after the ultraviolet irradiation time is prolonged, so that the cerasus humilis juice has stable properties under the ultraviolet irradiation.

In conclusion, the cerasus humilis juice has stable properties under high temperature, severe cold and ultraviolet irradiation, and is not easy to be influenced by external conditions to generate phenomena of precipitation, color change, oxidation, deterioration and the like, so the cerasus humilis juice can be applied to the preparation and production of cosmetics.

Example 3 preparation of cerasus humilis mask

Example 4 preparation of cerasus humilis mask

Essentially the same formulation as in example 3, except that the cerasus humilis juice content is 2%.

Example 5 preparation of cerasus humilis mask

Essentially the same formulation as in example 3, except that the cerasus humilis juice content is 10%.

Example 6 preparation of cerasus humilis mask

Essentially the same formulation as in example 3, except that the cerasus humilis juice content is 15%.

Example 7 preparation of a Prunus humilis Bunge Patch

EXAMPLE 8 preparation of cerasus humilis ointment

Example 9 allergy test on Prunus humilis Bunge mask

The cerasus humilis facial masks prepared in examples 3-6 are respectively selected to be subjected to human body experiments, and the results show that when the content of cerasus humilis juice is below 5%, the cerasus humilis facial masks are smeared on the surface of skin and have no sense; when the content of Prunus humilis Bunge juice is 15%, it can cause irritation to skin, and can cause skin redness, swelling, itching, and pain.

The result shows that when the content of the cerasus humilis juice is within the range of 5-10%, the cerasus humilis juice is smeared on the skin to have cool and lubricating feeling and has no other adverse phenomena.

Example 10 Prunus humilis Bunge mask whitening Effect test

1 test subject

1.1 inclusion conditions: (1) normal volunteers between 18-60 years of age; (2) an inactive allergic disease; (3) those who have no history of allergy to skin care cosmetics in the past; (4) volunteers participate and can complete the specified contents according to the experiment requirements.

1.2 number of subjects: in 12 cases, 6 boys and 6 girls aged 20-30 years old.

2, sample: cerasus humilis juice facial masks prepared in examples 3 and 4 (cerasus humilis juice content 2% and 5%, respectively) and a control facial mask without cerasus humilis juice (cerasus humilis juice amount supplemented with water, the rest ingredients being the same).

3 smearing of the sample

The smearing of the sample is taken by a specially-assigned person, the sample is smeared once in the morning and at night every day, and data are collected once every 7 days for 14 days.

4 Mexameter18 Refocal melanin measuring instrument collects data, wherein a piece of skin with uniform skin color and an area of about 4 cm × 4 cm is selected on the inner side of the upper arm on one side of a subject and is coated with a Prunus humilis Bunge juice facial mask; the inner side of the upper arm on the other side is coated with control facial mask. The probe is lightly placed on the selected skin to irradiate, and the melanin value of the skin on the upper arms of the two sides of the subject is determined.

5 results of melanin value measurement

The results of the skin melanin value measurements are shown in tables 4 and 5, and fig. 1 and 2. The results show that the melanin value of the cerasus humilis juice-coated facial mask is reduced all the time, and on the 14 th day of the cerasus humilis juice-coated facial mask, the melanin value of a cerasus humilis juice 5% treated group is obviously lower than that of a control group, while the melanin value of a cerasus humilis juice 2% treated group is not obviously different from that of the control group. The skin melanin value measurement result shows that the mask containing 5% of cerasus humilis juice has the whitening effect.

TABLE 4 determination of skin melanin value after applying 2% cerasus humilis juice mask

Days after application of mask	Cerasus humilis facial mask group	Control group
				0	173.8±8	175.2±9
7	170.5±6	171.2±11
			14	168.7±7	184.5±9

TABLE 5 determination of skin melanin value after applying 5% cerasus humilis juice mask

Days after application of mask	Cerasus humilis facial mask group	Control group
				0	173.1±11	175.2±9
7	168.4±8	171.2±11
			14	162.9±7	184.5±9 *

Note: means significant level of variabilityP<0.05。

Claims

1. The application of the cerasus humilis fruit juice in preparing the cosmetic or the pharmaceutical preparation with the whitening effect for external use is characterized in that the cerasus humilis fruit juice is prepared by pressing the cerasus humilis fruit; the soluble solid content of the cerasus humilis juice is 10% -21%; the Prunus humilis Bunge juice is effective in whitening skin;

the preparation method of the cerasus humilis juice comprises the following steps:

step a, selecting materials and juicing: crushing fully mature cerasus humilis fruits by using a crusher, and juicing by using a juicer;

step b, fine filtration: filtering the juice obtained in the step a by using a plurality of layers of gauze;

step c, clarification: freezing the finely filtered juice at 0 deg.C for a week, clarifying, and filtering to obtain clarified juice;

step e, sterilizing and cooling: and c, sealing the clear juice obtained in the step c, putting the sealed clear juice into a sterilization pot, sterilizing the clear juice for 30 seconds at the temperature of 80 ℃, taking the clear juice out, and cooling the clear juice to be below 38 ℃.

2. Use according to claim 1, wherein the cosmetic or pharmaceutical preparation comprises a mask, a patch, a lotion, an ointment, a cream.

3. The use of claim 1, wherein the cerasus humilis juice has a soluble solids content of 13% to 17%.

4. The cerasus humilis mask is characterized in that the content of cerasus humilis juice in the mask is 5% -10%;

wherein the cerasus humilis juice is prepared from cerasus humilis fruits by squeezing process; the soluble solid content of the cerasus humilis juice is 10% -21%;

5. The mask of claim 4, wherein the cerasus humilis juice soluble solids content is from 13% to 17%.

6. The mask of claim 4 wherein the cerasus humilis juice is present in an amount of 5% to 8%.

7. The cerasus humilis mask is characterized in that the mask comprises the following raw materials and auxiliary materials: 4-10% of cerasus humilis juice, 2-6% of 1, 3-butanediol, 0.2-0.6% of xanthan gum, 0.05-0.2% of dipotassium glycyrrhizinate, 1-4% of glycerol, 0.05-0.2% of allantoin, 0.1-0.5% of water-soluble jojoba oil, 0.3-1% of propylene glycol and the balance of water;

8. The mask as claimed in claim 7, wherein the mask comprises the following raw materials: 4-8% of cerasus humilis fruit juice, 3-5% of 1, 3-butanediol, 0.3-0.5% of xanthan gum, 0.08-0.15% of dipotassium glycyrrhizinate, 1-3% of glycerol, 0.08-0.15% of allantoin, 0.2-0.4% of water-soluble jojoba oil, 0.4-0.8% of propylene glycol and the balance of water.

9. The mask as claimed in claim 7, wherein the mask comprises the following raw materials: 5% of cerasus humilis juice, 4% of 1, 3-butanediol, 0.4% of xanthan gum, 0.1% of dipotassium glycyrrhizinate, 2% of glycerol, 0.1% of allantoin, 0.3% of water-soluble jojoba oil, 0.5% of propylene glycol and the balance of water.