CN108776979A - Utilize the method for image measurement plating medium bacterial strain diameter - Google Patents
Utilize the method for image measurement plating medium bacterial strain diameter Download PDFInfo
- Publication number
- CN108776979A CN108776979A CN201810722188.9A CN201810722188A CN108776979A CN 108776979 A CN108776979 A CN 108776979A CN 201810722188 A CN201810722188 A CN 201810722188A CN 108776979 A CN108776979 A CN 108776979A
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- point
- image
- tablet
- profile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 79
- 238000007747 plating Methods 0.000 title claims abstract description 23
- 238000005259 measurement Methods 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims abstract description 18
- 238000004364 calculation method Methods 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- 238000011282 treatment Methods 0.000 claims abstract description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 241001292396 Cirrhitidae Species 0.000 description 1
- 108010068977 Golgi membrane glycoproteins Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
Classifications
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/60—Analysis of geometric attributes
- G06T7/62—Analysis of geometric attributes of area, perimeter, diameter or volume
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T5/00—Image enhancement or restoration
- G06T5/90—Dynamic range modification of images or parts thereof
- G06T5/92—Dynamic range modification of images or parts thereof based on global image properties
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/12—Edge-based segmentation
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/13—Edge detection
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/136—Segmentation; Edge detection involving thresholding
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10056—Microscopic image
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Theoretical Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Geometry (AREA)
- Image Processing (AREA)
Abstract
The invention discloses a kind of method using image measurement plating medium bacterial strain diameter, one, obtain to carrying out image taking by the bacterium colony of culture after plating and include the tablet image A of the bacterium colony；Two, gray proces are carried out to tablet image A1, obtains tablet image A2；Three, binary conversion treatment is carried out to tablet image A2；Four, each corresponding profile of bacterial strain in tablet image A3 is found, and the corresponding profile of each bacterial strain is scanned, coboundary point, lower boundary point, left margin point, the right margin point of profile corresponding to each bacterial strain is found, calculates the length and width of each bacterial strain；The diameter calculation formula of bacterial strain be R2=（P2/P1）×R1；In formula：R2 is bacterial strain diameter；P2 is the number of pixels that bacterial strain width accounts in the picture；P1 is the number of pixels of plating medium in the picture；R1 is plate diameter.The invention has the advantages that realizing mass, the intellectualized operation measured colony diameter.
Description
Technical field
The present invention relates to the measurements of plating medium bacterial strain diameter, more particularly, to utilization image measurement plating medium bacterium
The method of strain diameter.
Background technology
Strain is connected on plating medium with oese by plating, or with pipette, dropper by certain volume
Bacterium solution moves on plating medium, then cultivates.The purpose of plating is observation colonial morphology, isolates and purifies strain, viable bacteria
Count and carried out on tablet various experiments.Plating medium forms circular colonies by inoculation, microorganism through outgrowth, leads to
The position for identifying single bacterium colony, size are crossed, then measures the diameter of bacterium colony for subsequent detection and analysis, experiment.Currently,
Measurement to colony diameter, it is common practice that doctor takes the cultured bacterium colony of ruler hand dipping, this is easy for causing to generate regarding
Feel error, measurement accuracy and efficiency are low, and it is very inconvenient to measure operation.Also, some bacterium colonies connect together after culture
The group's of being formed shape bacterium colony or bacterium colony in blocks, cause doctor that can not take measurement of the ruler to single bacterium colony.
Invention content
Present invention aims at provide a kind of method using image measurement plating medium bacterial strain diameter.
To achieve the above object, the present invention takes following technical proposals：
Method of the present invention using image measurement plating medium bacterial strain diameter carries out as steps described below：
The first step obtains the tablet image A for including the bacterium colony to carrying out image taking by the bacterium colony of culture after plating；
Second step carries out gray proces to the tablet image A1, obtains the tablet image A2 after gray proces；
Automatic threshold function in third step, library of being increased income using image procossing（AdaptiveThreshold）To the lithograph
As A2 progress binary conversion treatments, i.e.,：
Tablet image A2 is scanned through, and finds out maximum gradation value Rmax and minimum gradation value Rmin, and it is median, meter to take threshold value T
Calculating formula is：T=(Rmax+Rmin)/2, then scan image, is handled the gray value of each pixel, gray value is more than threshold value
T then becomes 255 whites, and gray value, which is less than threshold value T, then becomes 0 black, tablet image A3 is obtained, to make the tablet image A3
There is only black (gray value 0), white (gray values 255) in picture；
Lookup profile function in 4th step, library of being increased income by image procossing（FindContours）, find in tablet image A3
The corresponding profile of each bacterial strain, and the corresponding profile of each bacterial strain is scanned, find profile corresponding to each bacterial strain
Coboundary point, lower boundary point, left margin point, right margin point；The boundary point includes X-coordinate, Y coordinate and pixel
Number；Then the length and width of each bacterial strain is calculated separately out；
Since bacterial strain is rounded, length and width is equal, and the width of bacterial strain is shared number of pixels in the picture；Therefore, bacterial strain
Diameter calculation formula be R2=（P2/P1）×R1；In formula：R2 is bacterial strain diameter；P2 is bacterial strain width shared picture in the picture
Plain number；P1 is the number of pixels of plating medium in the picture；R1 is plate diameter；
The horizontal distance of the left margin point of bacterial strain profile to tablet left margin is the left margin point X-coordinate of bacterial strain profile；Bacterial strain wheel
The horizontal distance of wide right margin point to tablet left margin is the right margin point X-coordinate of bacterial strain profile；The coboundary of bacterial strain profile
The vertical range of point to tablet coboundary is the coboundary point Y coordinate of bacterial strain profile；On the lower boundary point to tablet of bacterial strain profile
The vertical range on boundary is the lower boundary point Y coordinate of bacterial strain profile；
The length of bacterial strain is：The lower boundary point Y coordinate of bacterial strain subtracts coboundary point Y coordinate；
The width of bacterial strain is：The right margin point X-coordinate of bacterial strain subtracts the X-coordinate of left margin point.
Tablet image A described in the first step is JPG, BMP or PNG picture format.
The invention has the advantages that realizing mass, the intellectualized operation measured colony diameter；Substantially increase doctor's work
Make efficiency, reduces measurement error.
Description of the drawings
Fig. 1 is the schematic diagram of tablet image A1 of the present invention.
Fig. 2 is the schematic diagram of tablet image A2 of the present invention.
Fig. 3 is the schematic diagram of tablet image A3 of the present invention.
Specific implementation mode
It elaborates below in conjunction with the accompanying drawings to the embodiment of the present invention, the present embodiment before being with technical solution of the present invention
It puts and is implemented, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to down
State embodiment.
Method of the present invention using image measurement plating medium bacterial strain diameter carries out as steps described below：
The first step obtains the tablet image A for including the bacterium colony to carrying out image taking by the bacterium colony of culture after plating,
As shown in Figure 1；
Second step carries out gray proces to the tablet image A1, obtains the tablet image A2 after gray proces, as shown in Figure 2；
Value gray=0.299 × 0.114 × blue of red+0.587 × green+ of the tablet image A2 pixels；
In formula：Red is the red color channel value of tablet image A1 pixels before gray proces；
Green is the green channel value of tablet image A1 pixels before gray proces；
Blue is the blue channel value of tablet image A1 pixels before gray proces；
Automatic threshold function in third step, library of being increased income using image procossing（AdaptiveThreshold）To the lithograph
As A2 progress binary conversion treatments, i.e.,：
Tablet image A2 is scanned through, and finds out maximum gradation value Rmax and minimum gradation value Rmin, and it is median, meter to take threshold value T
Calculating formula is：T=(Rmax+Rmin)/2, then scan image, is handled the gray value of each pixel, gray value is more than threshold value
T then becomes 255 whites, and gray value, which is less than threshold value T, then becomes 0 black, obtains tablet image A3, as shown in Figure 3；It is described to make
There is only black (gray value 0), white (gray values 255) in tablet image A3 pictures；
Lookup profile function in 4th step, library of being increased income by image procossing（FindContours）, find in tablet image A3
The corresponding profile of each bacterial strain, and the corresponding profile of each bacterial strain is scanned, find profile corresponding to each bacterial strain
Coboundary point, lower boundary point, left margin point, right margin point；The boundary point includes X-coordinate, Y coordinate；Then it counts respectively
Calculate the length and width of each bacterial strain；
Since bacterial strain is rounded, length and width is equal, and the width of bacterial strain is shared number of pixels in the picture；Therefore, bacterial strain
Diameter calculation formula be R2=（P2/P1）×R1；In formula：R2 is bacterial strain diameter；P2 is bacterial strain width shared picture in the picture
Plain number；P1 is the number of pixels of plating medium in the picture；R1 is plate diameter；
The horizontal distance of the left margin point of bacterial strain profile to tablet left margin is the left margin point X-coordinate of bacterial strain profile；Bacterial strain wheel
The horizontal distance of wide right margin point to tablet left margin is the right margin point X-coordinate of bacterial strain profile；The coboundary of bacterial strain profile
The vertical range of point to tablet coboundary is the coboundary point Y coordinate of bacterial strain profile；On the lower boundary point to tablet of bacterial strain profile
The vertical range on boundary is the lower boundary point Y coordinate of bacterial strain profile；
The length of bacterial strain is：The lower boundary point Y coordinate of bacterial strain subtracts coboundary point Y coordinate；
The width of bacterial strain is：The right margin point X-coordinate of bacterial strain subtracts the X-coordinate of left margin point.
Tablet image A described in the first step is JPG, BMP or PNG picture format.
The image procossing library of increasing income for seeking profile includes OpenCV, imageMagic, GraphicsMagick,
ImageStone, GIMP, CxImage, LEADTOOLS, Halcon etc. are common several.
Claims (2)
1. a kind of method using image measurement plating medium bacterial strain diameter, it is characterised in that：It carries out as steps described below：
The first step obtains the tablet image A for including the bacterium colony to carrying out image taking by the bacterium colony of culture after plating；
Second step carries out gray proces to the tablet image A1, obtains the tablet image A2 after gray proces；
Tablet image A2 carries out binary conversion treatment described in automatic threshold function pair in third step, library of being increased income using image procossing,
I.e.：
Tablet image A2 is scanned through, and finds out maximum gradation value Rmax and minimum gradation value Rmin, and it is median, meter to take threshold value T
Calculating formula is：T=(Rmax+Rmin)/2, then scan image, is handled the gray value of each pixel, gray value is more than threshold value
T then becomes 255 whites, and gray value, which is less than threshold value T, then becomes 0 black, tablet image A3 is obtained, to make the tablet image A3
There is only black, whites in picture；
It is corresponding to find each bacterial strain in tablet image A3 for lookup profile function in 4th step, library of being increased income by image procossing
Profile, and being scanned to the corresponding profile of each bacterial strain finds the coboundary point, following of profile corresponding to each bacterial strain
Boundary's point, left margin point, right margin point；The boundary point includes X-coordinate, Y coordinate and pixel number；Then it calculates separately out
The length and width of each bacterial strain；
Since bacterial strain is rounded, length and width is equal, and the width of bacterial strain is shared number of pixels in the picture；Therefore, bacterial strain
Diameter calculation formula be R2=（P2/P1）×R1；In formula：R2 is bacterial strain diameter；P2 is bacterial strain width shared picture in the picture
Plain number；P1 is the number of pixels of plating medium in the picture；R1 is plate diameter；
The horizontal distance of the left margin point of bacterial strain profile to tablet left margin is the left margin point X-coordinate of bacterial strain profile；Bacterial strain wheel
The horizontal distance of wide right margin point to tablet left margin is the right margin point X-coordinate of bacterial strain profile；The coboundary of bacterial strain profile
The vertical range of point to tablet coboundary is the coboundary point Y coordinate of bacterial strain profile；On the lower boundary point to tablet of bacterial strain profile
The vertical range on boundary is the lower boundary point Y coordinate of bacterial strain profile；
The length of bacterial strain is：The lower boundary point Y coordinate of bacterial strain subtracts coboundary point Y coordinate；
The width of bacterial strain is：The right margin point X-coordinate of bacterial strain subtracts the X-coordinate of left margin point.
2. the method for utilizing image measurement plating medium bacterial strain diameter according to claim 1, it is characterised in that：The first step
Described in tablet image A be JPG, BMP or PNG picture format.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810722188.9A CN108776979A (en) | 2018-07-04 | 2018-07-04 | Utilize the method for image measurement plating medium bacterial strain diameter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810722188.9A CN108776979A (en) | 2018-07-04 | 2018-07-04 | Utilize the method for image measurement plating medium bacterial strain diameter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108776979A true CN108776979A (en) | 2018-11-09 |
Family
ID=64030893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810722188.9A Withdrawn CN108776979A (en) | 2018-07-04 | 2018-07-04 | Utilize the method for image measurement plating medium bacterial strain diameter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108776979A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109637056A (en) * | 2018-12-13 | 2019-04-16 | 深圳众宝城贸易有限公司 | A kind of artificial intelligence Supermarket account-settling system |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0837148B1 (en) * | 1996-10-21 | 2001-08-29 | Kawasaki Steel Corporation | Grain-oriented electromagnetic steel sheet |
| CN1829727A (en) * | 2003-03-21 | 2006-09-06 | 斯比凯克公司 | High viscosity xanthan polymer preparations |
| US20070254221A1 (en) * | 2004-12-24 | 2007-11-01 | Hi-Kuk Lee | Photosensitive resin composition, thin film panel made with photosensitive resin composition, and method for manufacturing thin film panel |
| CN104087650A (en) * | 2014-06-27 | 2014-10-08 | 华南理工大学 | Detecting method for sub-lethal yeast under action of pulsed electric fields based on image analysis |
| CN104849276A (en) * | 2015-05-20 | 2015-08-19 | 辽宁工程技术大学 | Granite three-dimensional microstructure re-building method based on pixel statistics |
| CN106447020A (en) * | 2016-09-13 | 2017-02-22 | 上海海事大学 | Intelligent bacterium colony counting method |
| CN106485313A (en) * | 2016-10-18 | 2017-03-08 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of quick Numeration of bacterium colony |
-
2018
- 2018-07-04 CN CN201810722188.9A patent/CN108776979A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0837148B1 (en) * | 1996-10-21 | 2001-08-29 | Kawasaki Steel Corporation | Grain-oriented electromagnetic steel sheet |
| CN1829727A (en) * | 2003-03-21 | 2006-09-06 | 斯比凯克公司 | High viscosity xanthan polymer preparations |
| US20070254221A1 (en) * | 2004-12-24 | 2007-11-01 | Hi-Kuk Lee | Photosensitive resin composition, thin film panel made with photosensitive resin composition, and method for manufacturing thin film panel |
| CN104087650A (en) * | 2014-06-27 | 2014-10-08 | 华南理工大学 | Detecting method for sub-lethal yeast under action of pulsed electric fields based on image analysis |
| CN104849276A (en) * | 2015-05-20 | 2015-08-19 | 辽宁工程技术大学 | Granite three-dimensional microstructure re-building method based on pixel statistics |
| CN106447020A (en) * | 2016-09-13 | 2017-02-22 | 上海海事大学 | Intelligent bacterium colony counting method |
| CN106485313A (en) * | 2016-10-18 | 2017-03-08 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of quick Numeration of bacterium colony |
Non-Patent Citations (1)
| Title |
|---|
| 武雪锋: "菌落自动计数系统的开发与研究", 《中国优秀硕士学位论文全文数据库信息科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109637056A (en) * | 2018-12-13 | 2019-04-16 | 深圳众宝城贸易有限公司 | A kind of artificial intelligence Supermarket account-settling system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10614286B2 (en) | Method of detecting gas-producing microbial colonies | |
| EP3336195B1 (en) | Method of differentiating microbial colonies in an image | |
| CN103020996A (en) | Lab-space-based detection method based on image color cast | |
| CN106485313B (en) | A kind of quick Numeration of bacterium colony | |
| CN107644210A (en) | Micro organism quantity evaluation method based on image procossing | |
| JP6550076B2 (en) | Method and sterility test apparatus for detecting microcolonies growing on a sample membrane or agarose medium | |
| CN108776979A (en) | Utilize the method for image measurement plating medium bacterial strain diameter | |
| CN110751635B (en) | Oral cavity detection method based on interframe difference and HSV color space | |
| JP2007110932A (en) | Automatic method for culturing and cell culture apparatus | |
| CN110866906A (en) | Three-dimensional culture human myocardial cell pulsation detection method based on image edge extraction | |
| CN101963666B (en) | Method for correcting intensity image distortion of streak tube imaging laser radar | |
| CN103667417A (en) | Method for detecting microbes in beverage | |
| CN104460104B (en) | The method for determining the edge of color filter block and the overlapping region of black matrix | |
| CN110400320A (en) | A kind of dividing method of electrowetting defect pixel | |
| CN112365435A (en) | Image dead pixel detection and edge-based restoration algorithm | |
| CN104881652A (en) | Row number automatic detection algorithm based on corn ear convexity characteristic | |
| CN113436214B (en) | Brinell hardness indentation circle measuring method and system and computer readable storage medium | |
| CN104036470B (en) | Image enhancing method for eliminating binary noisy points | |
| CN202485628U (en) | Corn ear characteristic measure apparatus | |
| CN108151808B (en) | A kind of steel pipe measuring device and method based on image procossing | |
| CN105886389A (en) | Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask | |
| CN105420342A (en) | Method for detecting content of coliforms in raw material gum | |
| CN114395471B (en) | High-resolution chromogenic plate and application thereof | |
| CN108871224A (en) | A kind of depth of groove detection method for LED glass lamp cup | |
| CN103955683B (en) | The method and system that a kind of lottery ticket identifier image is calibrated automatically |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181109 |
|
| WW01 | Invention patent application withdrawn after publication |