CN108770681A - A method of the storage environment by regulating and controlling Cucumber In Vitro bud is bloomed the loose powder time to control bud - Google Patents
A method of the storage environment by regulating and controlling Cucumber In Vitro bud is bloomed the loose powder time to control bud Download PDFInfo
- Publication number
- CN108770681A CN108770681A CN201810517792.8A CN201810517792A CN108770681A CN 108770681 A CN108770681 A CN 108770681A CN 201810517792 A CN201810517792 A CN 201810517792A CN 108770681 A CN108770681 A CN 108770681A
- Authority
- CN
- China
- Prior art keywords
- bud
- cucumber
- environment
- vitro
- time
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
It is bloomed the method for loose powder time by regulating and controlling the storage environment of Cucumber In Vitro bud to control bud the invention discloses a kind of.The method includes the following steps：The previous day, which adopts down, spits yellow bud, is put into big hermetic bag, is full of air, water is sprayed in sealing bag wall, makes to be covered with water droplet in hermetic bag, but do not flow down largely, then hermetic bag is put into 20 DEG C -25 DEG C of saturated humidity environment 10-20 hours, bud can open, then be transferred to spare in 4 DEG C of environment.The present invention carries out system research to the environment of blooming of Cucumber In Vitro male flower flower bud for the first time, blooming the loose powder time for bud is controlled by regulating and controlling the storage environment of in vitro bud, it can solve the problems, such as the various flowering asynchronisms educated in the production of hybrid seeds and breeding process, achieve the effect that reach highest hybrid seed yield within shortest breeding time, reduces breeding cost.
Description
Technical field
The present invention relates to a kind of store methods of Cucumber In Vitro bud, more particularly to a kind of by regulating and controlling Cucumber In Vitro bud
Storage environment bloom the method for loose powder time to control bud.The invention belongs to field of agricultural production technologies.
Background technology
Cucumber breeding for heterosis provides theoretical foundation for the development of cucumber hybrid, is also the raising of cucumber yield
It provides the foundation, cucumber production range is growing the continuous development for having driven cucumber hybrid.Holland, the U.S., Japan
And the Taiwan in China is countries and regions relatively advanced in the sub- research field of cucumber hybrid in the world now, these states
It is generation that family and area establish the serial industry of flourishing cucumber seeds on the development foundation of the agricultural economy based on this country
The sub- main body entrance source of cucumber hybrid of various countries of boundary.These countries and regions are shown by establishing breeding production base and new varieties
Model base constructs full-life technology service system.And the quality of hybrid seed and the cucumber character produced with the hybrid seed
Preferably, parent's homozygosity of hybrid seed is higher, has good genetic base, especially the ground production of hybrid seeds such as Japan and Taiwan
Considered critical pollination date, seat melon number in the process produce the seed of high quality for it and have established solid foundation.
It is reported that Cucumber Pollen is write a prescription in its natural state after 4~5h, the vigor of pollen just loses, and temperature gets over Gao Zeshou
Life more shortening (influence [J] China's Vegetables of the Storage Temperature on Cucumber Pollen Viability such as Li Guang, Deng Qiang, Wei Aimin, 2007,
(9):22-24).Li Guang et al. thinks：It is exactly cryopreservation bud effectively to extend the method for Pollen life-span.Low temperature slows down flower
Tube cell extends speed, but has no effect on pollen highest germination rate, and pollen is under 5 DEG C of low temperature, pollen activity duration longest,
But be also to have disadvantage, studies have found that the bud of cryopreservation is unfavorable for male flower opening and loose powder.Moreover, there is examination
It tests and points out, 18:The male flower pollen activity of 00 picking is apparently higher than 13:It was picked before 00.Picking bud can be to some extent in advance
Reduce Cucumber Pollen vigor (refreshing cucumber seeds pretreatment technology and pollen storage technique study [D] Northeast Agricultural Universities
.2013)。
Jia bright red has studied principal element (Jia bright red, the neat of Wang Shu, Liu Zhong for influencing tomato pollen vigor and hybrid seed yield
Influence the Tianjin principal element [J] agricultural sciences of tomato pollen vigor and hybrid seed yield, 2010 (3)), find 4 DEG C of low temperature, room temperatures
Dry and sunlight, which dries, all makes pollen germination rate reduce, and the difference between them is extremely notable.Preserve 2d's in 4 DEG C of driers
The sprouting ability of pollen still has 50% or more.The viability of pollen grain can embody the germination rate of pollen grain on the one hand, separately
On the one hand it is exactly the length of pollen tube, the strong easy pollination success of viability.Also there is research to think, pollen in dry conditions and
Under the conditions of 20~25 DEG C of room temperature two, pollen viability can keep 4~5d, and (the refined east the seeds company cherry and tomato of land-sea is miscellaneous
Seeding technique is handed over to implement operating instruction [Z] .2004:12.).The research of Wang Jiqing etc. thinks can only to survive after pollen grain is in vitro one
Section time (influence [J] the kinds of Wang Jiqing, Zhao Yueping, Qiao Baojian storage pollens and pollinated stage to tomato hybrid seeding effect
Son, 2006,25 (1):90-91.).
Research is found：Calcium ion is added into the arabidopsis pollen of freezing, the germination rate of pollen can be improved.This experiment
Conclusion for us from now on have additional nutrients improve pollen viability provide reliable scientific basis.Research in He Cheng days et al. is compared
Processing of drying in the shade, lamp are according to difference dry, between insulating box drying three.Experiment obtains, dries in the shade processing compared with other two,
Pollen activity is declined, further relatively, shines the more of the pollen activity decline for taking powder to be obtained.Illustrate due to ultraviolet
Line destroys and pollen activity is caused to significantly reduce, so sun-cured method is undesirable.And Stushnoff (1991) researchs 4
It is also obtained in the different storage practice of kind, the mode most beneficial for the storage of title monkey peach flower powder is exactly cold drying.Wang Wei etc.
People (2009) has found rape pollen being stored in liquid nitrogen, reuses the method gradually thawed, pollen activity is enable to reach
Keep highest.
Many research report low temperature drying conditions can significantly extend service life of pollen, but related Cucumber Pollen vigor and
The research report of bud storage is seldom.Wei Aimin etc. has studied influence of -40 DEG C of cryopreservations to Cucumber Pollen vigor, but -40
DEG C condition is difficult to apply to the production of hybrid seeds and puts into practice (Wei Aimin, Li Xiaoying, Fan Min, Du Shengli Cucumber Pollen Cord blood Primary Studies
Youth's science and technology Tianjin forum treasury, the Tianjin [J] .2001:Tianjin science tech publishing house, 2001.).
The yield and quality and cost problem of hybrid seed production of cucumber, annoying always breeders for many years, especially northern
The regional four seasons temperature difference is larger, the excellent new varieties of a character, frequent low, evening loose powder time because of night temperature during the production of hybrid seeds,
Pollen activity is low, and the factors such as Parent flowering asynchronism cause the production of hybrid seeds phase long, and seed production is low, of poor quality, is added significantly to the production of hybrid seeds
Cost, and then planting cost is increased, seriously affect the quality, yield and economic benefit of commodity melon, hence it is evident that hinder excellent
Universal and popularization the process of kind, in some instances it may even be possible to forfeit a new varieties.The problem of for Cucumber Seed Production, domestic and foreign scholars existed
A large amount of research, the vigor of Study of Pollens, the reserve temperature and storage time of pollen have been done in terms of pollen, and achieve centainly at
Achievement, but still fail a series of problems for fundamentally solving to encounter in terms of cucumber pollination.
Present invention seek to address that the problems in above-mentioned cucumber crossbreeding and production practices, inquire into control male flower and open loose powder
Time and environmental condition, regulate and control the open hour of in vitro male flower flower bud under the premise of ensureing pollen germination rate, met with this
The demand of different pollination times improves the germination rate and seed production of pollen, ensures the preparation of cross combination, the preservation of material,
New theoretical foundation and methods for using them is provided for breed cucumber and hybrid seeding, meanwhile, it reduces production of hybrid seeds recruitment cost and increases kind
Thus suboutput improves the economic and social benefits.
Invention content
The purpose of the present invention is to provide one kind can be under the premise of ensureing pollen germination rate, by regulating and controlling Cucumber In Vitro
The storage environment of bud is bloomed the method for loose powder time to control bud.
In order to achieve the above object, present invention employs following technological means：
In recent years, inventor has done numerous studies in terms of male flower flower bud Plantlet in vitro.Autumn stubble material add for when,
At dusk 16:Adopted when 00 will open within second day spit yellow male flower flower bud, be placed under different temperature conditions and preserve, m seq male flower
It does not open loose powder, then again preserves picking bud in different humidity conditions, repetition test, discovery has part bud to open
It puts, thus proposes, in vitro male flower flower bud to be made to open, in addition to meeting certain temperature condition, the height of humidity is also heavy to closing
It wants.
Therefore, on the basis of above-mentioned research repeatedly, the present invention proposes a kind of storage by regulating and controlling Cucumber In Vitro bud
Dis environment is bloomed the method for loose powder time to control bud, is included the following steps：
The previous day, which adopts down, spits yellow cucumber bud, is put into big hermetic bag, is full of air, seals and sprays water in bag wall,
Make to be covered with water droplet in hermetic bag, but do not flow down largely, then hermetic bag is put into 10- in 20 DEG C -25 DEG C of saturated humidity environment
20 hours, bud opened, then is transferred to spare in 4 DEG C of environment.
Wherein, it is preferred that be the saturated humidity environment that hermetic bag is put into 25 DEG C of environment 12 hours, or is put into 20 DEG C
In 20 hours.
Wherein, it is preferred that the time that the previous day adopts down the cucumber bud for spitting Huang is 12:When 00 or 18:When 00.
Wherein, it is preferred that the time stored in 4 DEG C of environment is no more than 48 hours.
Compared to the prior art, the beneficial effects of the invention are as follows：
The present invention carries out system research to the environment of blooming of Cucumber In Vitro male flower flower bud for the first time, by the storage for regulating and controlling in vitro bud
Dis environment controls blooming the loose powder time for bud, can solve asking for the various flowering asynchronisms educated in the production of hybrid seeds and breeding process
Topic, achievees the effect that reach highest hybrid seed yield within shortest breeding time, reduces breeding cost.
Description of the drawings
Fig. 1 is that Cucumber In Vitro bud is placed on the bud opening status in 20 DEG C of saturated humidity environment after lower 20 hours.
Specific implementation mode
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.But examples are merely exemplary for these, and it is not intended to limit the scope of the present invention in any way.People in the art
Member it should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and form into
Row modifications or substitutions, but these modifications and replacement are each fallen in protection scope of the present invention.
Embodiment 1
On March 20th, 2018 starts to test, and whens noon 12 acquires the cucumber male flower flower bud of unopened three kinds respectively, point
Not Wei Fruit Cucumber H1141, hua ' nan-type cucumber T131, North-China Type cucumber 1061, each kind adopts 10 buds, is respectively put into big
Hermetic bag in, be full of air, seal bag wall on spray water, make to be covered with water droplet in hermetic bag, but do not flow down largely, then close
Envelope is put into 20 DEG C of saturated humidity environment, the open result of the next morning statistics.The results are shown in Table 1：
1 cucumber bud opening status of table counts
Embodiment 2
On March 24th, 2018 starts to test, and whens afternoon 6 acquires the cucumber male flower flower bud of unopened three kinds, respectively
Fruit Cucumber H1141, hua ' nan-type cucumber T131, North-China Type cucumber 1061, each kind adopt 10 buds, are respectively put into big close
In envelope, it is full of air, seals and sprays water in bag wall, make to be covered with water droplet in hermetic bag, but do not flow down largely, then hermetic bag
It is put into 25 DEG C of saturated humidity environment, the open result of the next morning statistics.The results are shown in Table 2：
2 cucumber bud opening status of table counts
3 pollination rate of embodiment counts
All open flowers that Examples 1 and 2 obtain are transferred in 4 DEG C of environment and are preserved, selection preserves the flower of different time
Pollination processing is carried out, counts pollination rate, the results are shown in Table 3：
3 pollination rate of table counts
The bud of Plantlet in vitro is put into as can be seen from Table 3 when preserving 48 in 4 DEG C of environment, the pollination rate of pollen is still
60% or more can be reached, illustration method can be solved for during hybrid seeding, because night in spring and autumn temperature is too low, causing morning male
Flower cannot open, to influence pollinate the production of hybrid seeds the problem of.
Claims (4)
1. a kind of bloomed the method for loose powder time by regulating and controlling the storage environment of Cucumber In Vitro bud to control bud, feature exists
In including the following steps：
The previous day, which adopts down, spits yellow cucumber bud, is put into big hermetic bag, is full of air, seals and sprays water in bag wall, makes close
It is covered with water droplet on envelope, but does not flow down largely, it is small then hermetic bag is put into 10-20 in 20 DEG C -25 DEG C of saturated humidity environment
When, bud opens, then is transferred to spare in 4 DEG C of environment.
2. the method as described in claim 1, which is characterized in that it is small hermetic bag to be put into 25 DEG C of saturated humidity environment 12
When, or be put into 20 DEG C of saturated humidity environment 20 hours.
3. the method as described in claim 1, which is characterized in that the time that the previous day adopts down the cucumber bud for spitting Huang is 12:00
When or 18:When 00.
4. the method as described in claim 1, which is characterized in that the time stored in 4 DEG C of environment is no more than 48 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810517792.8A CN108770681A (en) | 2018-05-25 | 2018-05-25 | A method of the storage environment by regulating and controlling Cucumber In Vitro bud is bloomed the loose powder time to control bud |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810517792.8A CN108770681A (en) | 2018-05-25 | 2018-05-25 | A method of the storage environment by regulating and controlling Cucumber In Vitro bud is bloomed the loose powder time to control bud |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108770681A true CN108770681A (en) | 2018-11-09 |
Family
ID=64027619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810517792.8A Pending CN108770681A (en) | 2018-05-25 | 2018-05-25 | A method of the storage environment by regulating and controlling Cucumber In Vitro bud is bloomed the loose powder time to control bud |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108770681A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022109323A (en) * | 2019-11-22 | 2022-07-27 | カゴメ株式会社 | Stored pollen and method for producing the same as well as method for producing tomato |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1652680A (en) * | 2002-05-14 | 2005-08-10 | 辛根塔参与股份公司 | Tetraploid watermelon producing small fruits |
| US20150257345A1 (en) * | 2012-11-28 | 2015-09-17 | Pollen-Tech Llc | Pollen compositiions and methods for distribution on flowering plants |
| CN105875229A (en) * | 2016-04-29 | 2016-08-24 | 华南农业大学 | Seedling culture method for promoting precocious flowering of cucumber |
| CN106922519A (en) * | 2017-03-09 | 2017-07-07 | 金寨胜达农业科技有限公司 | A kind of method of the quick emasculation crossbreeding of cucumber |
-
2018
- 2018-05-25 CN CN201810517792.8A patent/CN108770681A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1652680A (en) * | 2002-05-14 | 2005-08-10 | 辛根塔参与股份公司 | Tetraploid watermelon producing small fruits |
| US20150257345A1 (en) * | 2012-11-28 | 2015-09-17 | Pollen-Tech Llc | Pollen compositiions and methods for distribution on flowering plants |
| CN105875229A (en) * | 2016-04-29 | 2016-08-24 | 华南农业大学 | Seedling culture method for promoting precocious flowering of cucumber |
| CN106922519A (en) * | 2017-03-09 | 2017-07-07 | 金寨胜达农业科技有限公司 | A kind of method of the quick emasculation crossbreeding of cucumber |
Non-Patent Citations (5)
| Title |
|---|
| PALUPI, E. R.等: ""Pollen drying and storage for hybrid seed production of cucumber (Cucumis sativus L.)"", 《ACTA HORTICULTURAE》 * |
| 于海霞等: ""不同贮藏方法对黄瓜花粉活力的影响"", 《北方园艺》 * |
| 李光等: ""贮藏温度对黄瓜花粉活力的影响"", 《中国蔬菜》 * |
| 许文连等: ""黄瓜花粉萌发的实验"", 《蔬菜》 * |
| 许蕊仙等: "《蔬菜良种繁育技术》", 30 June 1986, 黑龙江科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022109323A (en) * | 2019-11-22 | 2022-07-27 | カゴメ株式会社 | Stored pollen and method for producing the same as well as method for producing tomato |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Emershad et al. | In‐ovulo embryo culture of Vitis vinifera LCV ‘Thompson seedless’ | |
| US10398099B2 (en) | Grain production | |
| Teixeira da Silva et al. | Tissue culture and micropropagation of tree peony (Paeonia suffruticosa Andr.) | |
| CN109006455B (en) | Method for improving seed setting rate of konjac glucomannan and amorphophallus konjac | |
| CN105075777B (en) | A kind of blueberry liver moss seedling mating system | |
| Lei et al. | Impact of chilling accumulation and hydrogen cyanamide on floral organ development of sweet cherry in a warm region | |
| Thammasiri | Cryopreservation of some Thai orchid species | |
| CN112088770B (en) | High-activity pollen suspension for spray pollination of Korla pear tree and preparation and pollination methods thereof | |
| CN108770681A (en) | A method of the storage environment by regulating and controlling Cucumber In Vitro bud is bloomed the loose powder time to control bud | |
| Oren-Shamir et al. | Temperature and gibberellin effects on growth and anthocyanin pigmentation in Photinia leaves | |
| CN114128706B (en) | Pineapple pollination liquid and hybrid pollination method | |
| Matsuda et al. | Anatomical study for critical high temperature on the anthesis day to inhibit passion fruit set | |
| Dixit et al. | Cryopreservation: a potential tool for long-term conservation of medicinal plants | |
| AU2020101771A4 (en) | Method for in vitro cyclic preservation of Potato Germplasm | |
| CN110558216B (en) | Eustoma russellianum seedling growing method for eliminating rosette characters through temperature and light regulation | |
| MATSUDA et al. | Anatomical study on seasonal changes in pistil receptivity of cherimoya (Annona cherimola Mill.) | |
| CN103053347A (en) | Winter greenhouse grafting method for English hawthorn 'Scarlet' planted in Beijing area | |
| CN115152445B (en) | Method for artificial hybridization breeding by utilizing Yang Shuxi short flowering branches | |
| Fawusi | Germination of Talinum triangulare L. seeds as affected by various chemical and physical treatments | |
| CN111887154B (en) | Method for cryopreservation of embryonic callus of iris germanica | |
| CN109479704A (en) | A kind of method of precocity peento rescue culture | |
| NL2032340B1 (en) | BUD-GERMINATING AND SEEDLING-RAISING METHOD FOR HELLEBORUSxHYBRIDUS SEED | |
| CN110476536A (en) | A kind of tulip seed balls postharvest handling and forcing culture method | |
| Pooja et al. | Sterilization, germination and shoot proliferation from Chrysanthemum coronarium L. seeds. | |
| Frett et al. | Effect of nitrogen and calcium stock plant nutrition on Petunia× hybrida leaf and anther explant growth in vitro |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181109 |