CN108753307A - A kind of renovation agent for administering lead-contaminated soil - Google Patents
A kind of renovation agent for administering lead-contaminated soil Download PDFInfo
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- CN108753307A CN108753307A CN201810586254.4A CN201810586254A CN108753307A CN 108753307 A CN108753307 A CN 108753307A CN 201810586254 A CN201810586254 A CN 201810586254A CN 108753307 A CN108753307 A CN 108753307A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of renovation agents for administering lead-contaminated soil, include the raw material of following parts by weight：30-50 parts of water, 10-20 parts of PH7.2 phosphate buffers, 5-7 parts of bare glass fly pole strain, 5-7 parts of the thermophilic lead strain in chicken source, 3-5 parts of glucose, 3-5 parts of tryptone, 3-5 parts of yeast extract, 2-4 parts of skimmed milk power, 2-4 parts of glycerine, 2-4 parts of maltodextrin, 2-4 parts of trehalose, 2-4 parts of sodium glutamate.The present invention can be effectively reduced the content of lead in soil, achieve the purpose that the soil repaired, while environmentally friendly, avoid the secondary pollution that soil is caused when repairing.
Description
Technical field
The present invention relates to soil remediation range, specifically a kind of renovation agent for administering lead-contaminated soil.
Background technology
With the continuous quickening of process of industrialization, the unreasonable exploitation and its smelting of mineral resources are discharged, for a long time to soil
The reasons such as application of atmospheric sedimentation, chemical fertilizer and pesticide caused by sewage irrigation and sludge application, human activity are carried out, soil is caused
Earth is seriously polluted.
For the concrete condition of current domestic soil pollution, there is no specific official's data.Think, at present China
Soil pollution especially heavy metal pollution of soil have the tendency that being further aggravated, either from pollution level still from pollution model
It is such from the point of view of enclosing.Estimate accordingly, the farmland in China existing 1/6th is by heavy metal pollution at present, and China is as people
The very high country of mouthful density, the pollution in soil is very big to the health effect of people, soil contamination problem also gradually by
Pay attention to.Lead is a kind of common source of heavy metal pollution,.Currently mainly there is object for the reparation of Pb-Zn ore district soil pollution both at home and abroad
Reason, chemistry and bioremediation technology, wherein physical chemistry reparation because may caused by secondary pollution.And it is biological prosthetic without secondary dirt
It contaminates and expense is low, efficient, with the mainstream development direction as research.
The present invention proposes a kind of renovation agent for administering lead-contaminated soil, is based on bioremediation technology, can be effective
The lead content reduced in soil, and it is environmentally friendly, secondary pollution will not be caused.
Invention content
The purpose of the present invention is to provide a kind of renovation agents for administering lead-contaminated soil, to solve above-mentioned background technology
The problem of middle proposition.
To achieve the above object, the present invention provides the following technical solutions：
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：30-50 parts of water, PH7.2 phosphorus
10-20 parts of acid buffer, 5-7 parts of bare glass fly pole strain, 5-7 parts of the thermophilic lead strain in chicken source, 3-5 parts of glucose, tryptone
3-5 parts, 3-5 parts of yeast extract, 2-4 parts of skimmed milk power, 2-4 parts of glycerine, 2-4 parts of maltodextrin, 2-4 parts of trehalose, glutamic acid
2-4 parts of sodium.
As a further solution of the present invention：Include the raw material of following parts by weight：35-45 parts of water, PH7.2 phosphate buffers
15-17 parts, 5.5-6.5 parts of bare glass fly pole strain, 6.5-7 parts of the thermophilic lead strain in chicken source, 3.5-4.5 parts of glucose, tryptose
3.5-4.5 parts of peptone, 3.5-4.5 parts of yeast extract, 2.5-3.8 parts of skimmed milk power, 2.5-3.8 parts of glycerine, maltodextrin 2.5-3.8
Part, 2.5-3.8 parts of trehalose, 2.5-3.8 parts of sodium glutamate.
As further scheme of the invention：Include the raw material of following parts by weight：40 parts of water, PH7.2 phosphate buffers
16 parts, it is 6 parts of bare glass fly pole strain, 6 parts of the thermophilic lead strain in chicken source, 4 parts of glucose, 4 parts of tryptone, 4 parts of yeast extract, de-
3 parts of fat milk powder, 3 parts of glycerine, 3 parts of maltodextrin, 3 parts of trehalose, 3 parts of sodium glutamate.
The preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention can be effectively reduced the content of lead in soil, achieve the purpose that the soil repaired, while to environment
Close friend avoids the secondary pollution that soil is caused when repairing.
Specific implementation mode
The technical solution of this patent is described in more detail With reference to embodiment.
Embodiment 1
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：30 parts of water, PH7.2 phosphoric acid
10 parts of buffer solution, 5 parts of bare glass fly pole strain, 5 parts of the thermophilic lead strain in chicken source, 3 parts of glucose, 3 parts of tryptone, yeast extract
3 parts, 2 parts of skimmed milk power, 2 parts of glycerine, 2 parts of maltodextrin, 2 parts of trehalose, 2 parts of sodium glutamate.
In the present embodiment, the preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product.
Embodiment 2
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：32 parts of water, PH7.2 phosphoric acid
13 parts of buffer solution, 5.8 parts of bare glass fly pole strain, 5.8 parts of the thermophilic lead strain in chicken source, 3.5 parts of glucose, tryptone 3.5
Part, 3.5 parts of yeast extract, 2.8 parts of skimmed milk power, 2.8 parts of glycerine, 2.8 parts of maltodextrin, 2.8 parts of trehalose, sodium glutamate
2.8 part.
In the present embodiment, the preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product.
Embodiment 3
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：40 parts of water, PH7.2 phosphoric acid
16 parts of buffer solution, 6 parts of bare glass fly pole strain, 6 parts of the thermophilic lead strain in chicken source, 4 parts of glucose, 4 parts of tryptone, yeast extract
4 parts, 3 parts of skimmed milk power, 3 parts of glycerine, 3 parts of maltodextrin, 3 parts of trehalose, 3 parts of sodium glutamate.
In the present embodiment, the preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product.
Embodiment 4
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：46 parts of water, PH7.2 phosphoric acid
18 parts of buffer solution, 6.2 parts of bare glass fly pole strain, 6.2 parts of the thermophilic lead strain in chicken source, 4.2 parts of glucose, 4.2 parts of tryptone,
4.2 parts of yeast extract, 3.3 parts of skimmed milk power, 3.3 parts of glycerine, 3.3 parts of maltodextrin, 3.3 parts of trehalose, sodium glutamate 3.3
Part.
In the present embodiment, the preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product
Embodiment 5
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：48 parts of water, PH7.2 phosphoric acid
19 parts of buffer solution, 7 parts of bare glass fly pole strain, 7 parts of the thermophilic lead strain in chicken source, 4 parts of glucose, 4 parts of tryptone, yeast extract
4 parts, 3.2 parts of skimmed milk power, 3.2 parts of glycerine, 3.2 parts of maltodextrin, 3.2 parts of trehalose, 3.2 parts of sodium glutamate.
In the present embodiment, the preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product
Embodiment 6
A kind of renovation agent for administering lead-contaminated soil includes the raw material of following parts by weight：50 parts of water, PH7.2 phosphoric acid
20 parts of buffer solution, 7 parts of bare glass fly pole strain, 7 parts of the thermophilic lead strain in chicken source, 5 parts of glucose, 5 parts of tryptone, yeast extract
5 parts, 4 parts of skimmed milk power, 4 parts of glycerine, 4 parts of maltodextrin, 4 parts of trehalose, 4 parts of sodium glutamate.
In the present embodiment, the preparation method for administering the renovation agent of lead-contaminated soil includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender and is mixed in the environment of 20-30 DEG C
Stirring, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender in 40-45
It is mixed in the environment of DEG C, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, cultivates 15-20h at normal temperatures, during which use
PH7.2 phosphate buffers clean 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, allows suspending liquid A under normal temperature environment
3-5h is cultivated, thick liquid A is obtained
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, cultivates 15-20h at normal temperatures, during which use PH7.2
Phosphate buffer cleans 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, allows suspension B in normal temperature environment
Lower culture 3-5h, obtains thick liquid B
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it then will mixing
Object freeze-drying is got product.
Comparative example 1：Compared with Example 3, lack it is protectant produce, other are same as Example 3.
Comparative example 2：Compared with Example 3, lack the cleaning of PH7.2 phosphate buffers, other are same as Example 3.
Performance detection
Renovation agent obtained by embodiment 3 and comparative example 1,2 is applied to the detection of laggard quadrat content in soil, is obtained
To table 1.
The present invention can be effectively reduced the content of lead in soil, achieve the purpose that the soil repaired, while to environment
Close friend avoids the secondary pollution that soil is caused when repairing.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (4)
1. a kind of renovation agent for administering lead-contaminated soil, which is characterized in that include the raw material of following parts by weight：Water 30-50
Part, 10-20 parts of PH7.2 phosphate buffers, 5-7 parts of bare glass fly pole strain, 5-7 parts of the thermophilic lead strain in chicken source, glucose 3-5
Part, 3-5 parts of tryptone, 3-5 parts of yeast extract, 2-4 parts of skimmed milk power, 2-4 parts of glycerine, 2-4 parts of maltodextrin, trehalose 2-
4 parts, 2-4 parts of sodium glutamate.
2. the renovation agent according to claim 1 for administering lead-contaminated soil, which is characterized in that including following parts by weight
Raw material：35-45 parts of water, 15-17 parts of PH7.2 phosphate buffers, 5.5-6.5 parts of bare glass fly pole strain, the thermophilic lead bacterium in chicken source
6.5-7 parts of kind, 3.5-4.5 parts of glucose, 3.5-4.5 parts of tryptone, 3.5-4.5 parts of yeast extract, skimmed milk power 2.5-3.8
Part, 2.5-3.8 parts of glycerine, 2.5-3.8 parts of maltodextrin, 2.5-3.8 parts of trehalose, 2.5-3.8 parts of sodium glutamate.
3. the renovation agent according to claim 1 for administering lead-contaminated soil, which is characterized in that including following parts by weight
Raw material：40 parts of water, 16 parts of PH7.2 phosphate buffers, 6 parts of bare glass fly pole strain, 6 parts of the thermophilic lead strain in chicken source, glucose 4
Part, 4 parts of tryptone, 4 parts of yeast extract, 3 parts of skimmed milk power, 3 parts of glycerine, 3 parts of maltodextrin, 3 parts of trehalose, sodium glutamate
3 parts.
4. a kind of preparation method as described in any one of claims 1-3 for administering the renovation agent of lead-contaminated soil, feature
It is, includes the following steps：
1) glucose, tryptone and yeast extract are weighed, is put into blender in the environment of 20-30 DEG C mix and stir
It mixes, it is 5.7 then to adjust its pH value, obtains culture medium, culture medium is divided into two parts；
2) water, skimmed milk power, glycerine, maltodextrin, trehalose and sodium glutamate are weighed, is put into blender at 40-45 DEG C
It is mixed under environment, obtains protective agent, protective agent is divided into two parts；
3) it weighs bare glass fly pole strain to be put into a culture medium, 15-20h is cultivated at normal temperatures, during which with PH7.2 phosphorus
Acid buffer cleans 2-4 times；
4) a protective agent is added in the culture medium of step 4), obtains suspending liquid A, suspending liquid A is allowed to be cultivated under normal temperature environment
3-5h obtains thick liquid A；
5) it weighs the thermophilic lead strain in chicken source to be put into another culture medium, 15-20h is cultivated at normal temperatures, during which with PH7.2 phosphoric acid
Buffer solution for cleaning 2-4 times；
6) another protective agent is added in the culture medium of step 5), obtains suspension B, suspension B is allowed to be trained under normal temperature environment
3-5h is supported, thick liquid B is obtained；
7) thick liquid A and thick liquid B are put into blender under normal temperature environment and are mixed, it is then that mixture is cold
Dry get product is lyophilized.
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