CN108745433A - A kind of wide-range high-precision pipettor - Google Patents
A kind of wide-range high-precision pipettor Download PDFInfo
- Publication number
- CN108745433A CN108745433A CN201810833260.5A CN201810833260A CN108745433A CN 108745433 A CN108745433 A CN 108745433A CN 201810833260 A CN201810833260 A CN 201810833260A CN 108745433 A CN108745433 A CN 108745433A
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- CN
- China
- Prior art keywords
- piston
- piston cylinder
- liquid
- square
- liquid relief
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
Abstract
The invention discloses a kind of wide-range high-precision pipettors, it is characterised in that:Including piston, dialing knob, piston cylinder, liquid relief interface.Piston is made of spiral handspike and square bodily form teflon piston head, and piston head coincide with piston cylinder bore seal.Piston cylinder intracavity section is 1.0 centimeter sides square, has scale, 0.5 milliliter of minimum indexing outside piston cylinder.Dialing knob is made of symmetrical two parts, is screwed, and it is 10 microlitres that periphery, which has 50 equi-distance scale lines, minimum indexing,.There are two the different interfaces of diameter for liquid relief interface tool, and major diameter interface is for connecting heavy caliber liquid transfer gun head, and minor diameter interface is for connecting small-bore liquid transfer gun head or injection needle.The square internal diameter piston component of 1 square centimeter of the use of this pipettor novelty, liquid relief range is milliliter accurate adjustable from 10 microlitres to 10, has simple in structure, easy to operate, the advantage that liquid relief is accurate, vdiverse in function.
Description
【Technical field】
The present invention relates to experimental analysis fields to be related to a kind of wide-range particularly for laboratory pre-treatment analytical equipment
High-precision pipettor.
【Background technology】
In traditional laboratory chemical analysis, measures large volume liquid reagent or solution commonly uses graduated cylinder, measuring cup, capacity
Bottle etc., measures small volumes of liquids reagent or solution commonly uses graduated pipette or single mark pipette.With analysis science and technology development,
Follow-up developments are to pipettor, dispenser, syringe and liquid-transfering gun.Wherein syringe and liquid-transfering gun can be divided into constant and micro two again
Kind, and accurate measuring micro liquid generally utilizes micro syringe and micropipettor, because both measurers can be as accurate as
Microlitre level readings.There are many range specification of syringe and pipettor currently on the market, common range have 10 milliliters, 5 milliliters,
2 milliliters, 1 milliliter, 500 microlitres, 200 microlitres, 100 microlitres, 50 microlitres, 20 microlitres, 10 microlitres of equal-specifications.If pipetted accurate
Volume, often operated jointly using the pipettor of plurality of specifications.This in testing as needed to be accurate to measurement 15.385
Milliliter solution, needs point several steps to be operated:Constant syringe or 15 milliliters of liquid of pipette are first measured, then again with 1 milli
The syringe or pipettor 380 microlitres of liquid of measurement of liter or 500 microlitres of range specifications, finally need to use the other pipettor of micro updating
Or syringe measures 5 microlitres of liquid.At this time if there is a kind of wide scope adjusts range, high-precision, can once measure amount in place
Tool, then can save a large amount of manpower and materials and greatly improve conventional efficient and experimental precision.
Traditional liquid shifting equipment is all to be drawn using cylindrical piston component and liquid is discharged, and the volume for pipetting liquid is equal to
Plunger shaft sectional area is multiplied by piston and extracts distance, and cylindrical piston component has certain defect, since it is desired that its calculating is related to justifying
Frequency, and pi is an approximation, and due to the stroke distances of piston nor physical length, so last volume is real
It is a calibration number on border.If plunger shaft sectional area is known perfect number, such as 1 square centimeter, plunger shaft Accurate Calibration is real
Border length (i.e. piston shift motion is accurately readable), then pipetting volume can be accurately calculated completely.
Based on set forth above, invention is a kind of to have wide-range, while but also with the micro-injection pipettor that high-precision measures
It is very necessary.The pipettor can reduce and pipette number, use manpower and material resources sparingly and avoid the adduction error repeatedly measured, greatly
Width improves conventional efficient and experimental precision.
The invention discloses a kind of liquid relief ranges from 10 microlitres to 10 milliliter across 3 order of magnitude super-wide ranges, least count
Precision reaches the other pipettor of 10 micro updatings, and disposable accurate liquid relief is realized in range ability, piston component novelty
Using 1 square centimeter of square section, the unification of piston displacement and piston chamber length scale marker is realized so that pipette volume
It is more intuitive more accurate.The configuration of the present invention is simple, it is easy to use, replacing for numerous capacity and liquid relief measurer is can be used as in experimental analysis
For equipment.
【Invention content】
Narrow for the measurers working range such as existing graduated cylinder, measuring cup, pipette, pipettor, big volume measurer lacks precision,
Micro measurer repeats to pipette the limitation that will produce accumulated error again due to the small liquid relief inefficiency of capacity, and the present invention discloses
For a kind of liquid relief range milliliter across 3 order of magnitude super-wide ranges from 10 microlitres to 10, least count precision reaches 10 microlitres of ranks
Pipettor, disposable accurate liquid relief is met in range ability, laboratory liquid relief efficiency is realized and quality greatly improves, should
The shape, structure of invention and the technical solution taken are as follows:
A kind of wide-range high-precision pipettor, it is characterised in that:Including piston, dialing knob, piston cylinder, liquid relief interface.
The piston is made of spiral handspike and square bodily form teflon piston head, and piston head is connected sealing with piston tube inner chamber.
The piston cylinder intracavity section is 1 centimeter sides square, and piston cylinder front has volume scale, 0.5 milliliter of minimum indexing.Institute
It states dialing knob to be made of symmetrical two parts, be screwed, it is 10 that periphery, which has 50 equi-distance scale lines, minimum indexing,
Microlitre.There are two the different interfaces of diameter for the liquid relief interface tool.
Helpfulness effect:
The invention discloses a kind of liquid relief ranges from 10 microlitres to 10 milliliter across 3 order of magnitude super-wide ranges, least count
Precision reaches the other pipettor of 10 micro updatings, not only has bigger range ability, but also with accurate measurement precision, in its amount
Disposable accurate liquid relief can be achieved within the scope of journey, improve liquid relief efficiency and quality.Pipettor of the present invention is innovatively flat using 1
The piston component in square cm square shape section, realizes the unification of piston displacement and piston chamber length scale marker so that pipette
Volume is more intuitive more accurate.In addition, pipettor of the present invention innovatively uses big small-bore serial interface, constant shifting can be flexibly connected
Liquid pipette tips and Micro pipette tip, it might even be possible to connect injection needle, realize the diversification of purposes.The configuration of the present invention is simple,
It is easy to use, the alternate device of numerous capacity and liquid relief measurer is can be used as in experimental analysis.
【Description of the drawings】
Fig. 1:Stereogram exploded view of the present invention:1 piston;2 dialing knobs;3 piston cylinders;4 liquid relief interfaces.
Fig. 2:Front view of the present invention.
Fig. 3:Rearview of the present invention.
【Specific implementation mode】
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
The non-use scope for limiting the present invention.
Embodiment 1
Shown in (1) in the shape and structure such as Fig. 1 of the piston:Piston is by spiral handspike and square bodily form polytetrafluoroethyl-ne
Alkene piston head forms, and piston head is connected sealing with piston cylinder (3) inner cavity.Spiral handspike drives piston head movement in piston cylinder to reach
To the effect for drawing or being discharged solution.On spiral handspike have the uniform screw thread of spacing, flight pitch be 0.5 centimetre, specification with
Internal thread matching on dialing knob (2), dialing knob (2) is per precession or screws out one week, and band piston promotes in piston cylinder
Or pull out 0.5 centimetre.
In the shape and structure such as Fig. 1 of the dialing knob shown in (2):Dialing knob is made of symmetrical two parts, is led to
It crosses screw to fix, periphery has 50 equi-distance scale lines, meets 5 multiple to identify an Arabic numerals, dialing knob (2) is per precession
Piston (1) is pushed within one week to move 1 indexing (0.5 centimetre) along piston barrel chamber, pipettor of the present invention is discharged or draws 0.5 milliliter of liquid
Body, i.e. dialing knob (2) are per precession or screw out the corresponding discharge of 1 indexing (1/50th perimeter) or draw 10 microlitres (0.01) milli
Rise liquid.Internal thread on dialing knob and the screw thread on the spiral handspike of piston match, and fit together perfectly.Dialing knob passes through
Two screw tightenings are nested in piston cylinder (3) inlet after merging, annular groove is aligned the annular protrusion on piston cylinder.
In the shape and structure such as Fig. 1 of the piston cylinder shown in (3):Piston cylinder intracavity section is that 1.0 centimeter sides are square
Shape, piston cylinder (3) front have scale Digital ID:0,1,2,3,4,5,6,7,8,9,10, the graduation mark interval for having number is real
Border length is 1 centimetre, since piston cavity section is 1.0 centimeter sides square, so right between adjacent two digital calibrations line
The volume answered is 1 cubic centimetre (i.e. 1 milliliter), that is, the corresponding piston cylinder of graduation mark (3) cavity volume for having number is:0 milliliter
1, milliliter, 2 milliliters, 3 milliliters, 4 milliliters, 5 milliliters, 6 milliliters, 7 milliliters, 8 milliliters, 9 milliliters, 10 milliliters.0.5 milli of minimum indexing
It rises.Piston cylinder (3) inlet has twice annular protrusion, specification strictly to match, be nested with one another with the groove on dialing knob (2)
Afterwards, it fits together perfectly.
In the shape and structure such as Fig. 1 of the liquid relief interface shown in (4):The liquid relief interface and piston cylinder (3) exit
Zero graduation position connection (as shown in Figure 2), tool there are two the different interface of diameter, major diameter interface be used for 1 milliliter with
Upper constant liquid relief, connects heavy caliber liquid transfer gun head, and minor diameter interface is used for 1 milliliter or less micropipette, connects small-bore liquid relief
Pipette tips or injection needle.The liquid relief interface is to organic solution inert material, and minor diameter interface larger diameter interface grows 0.5 li
Rice.
Embodiment 2
In detecting health medicine in the experiment of silaenafil content, configuration concentration is that standard female of the west ground of 100mg/kg
Solution only has 25mg, purity there was only 98.5% due to buying silaenafil reference substance, and more expensive, very much with ten
One balance weighs 5.3mg and is placed in reagent bottle, is computed, and needs addition 5.12ml methanol solutions to be dissolved, traditional operation side
Method is in two steps:1, first 5.0 milliliters are measured with 5 milliliters of pipette or liquid-transfering gun to be added in reagent bottle (because of the scale of pipette
Reading, is observed, by visual observation so there are human errors), 2, with 1 milliliter of range or 200 microlitres of range liquid-transfering guns pipette 120 again
Microlitre methanol is added in reagent bottle that (uncertainty that the operation of this two step generates is cumulative).If using pipettor of the present invention,
Only need single stepping:Heavy caliber liquid transfer gun head is selected to be mounted on the major diameter interface of liquid relief interface (4) of the present invention, up time
Needle turns dialing knob (2), and dialing knob (2) pushes piston (1) until its piston head stops at the zero graduation of plunger shaft bottom
Line position (as shown in Figure 2), " 0 " graduation mark on dialing knob should be directed at piston cylinder (3) high scale vertical line and (be zeroed at this time
State), then liquid transfer gun head is immersed in the methanol solution of reagent bottle, turn dialing knob counterclockwise, pushes piston (1) stress
Methanol solution is drawn outwardly, and dialing knob often turns 1 week, and piston head rises 1 scale (0.5ml) along piston cylinder, persistently turns
Dialing knob (2) slows down when graduation mark shown in piston head lower end is more than " 5ml " and turns speed, and observation piston cylinder (3) is perpendicular
Graduation mark position on straight graduation mark alignment dialing knob (2), when it is directed at the 12nd article of graduation mark on dialing knob (2),
Stopping is exerted oneself, and the methanol solution volume that liquid-transfering gun of the present invention is drawn at this time is just 5.12ml, then turns scale rotation clockwise
Button (2) is returned clean state and accurate be added of the methanol pipetted can be loaded in the reagent bottle of silaenafil standard substance.Entirely
Pipetting processes, it is succinct intuitive, it is time saving and energy saving.
Claims (2)
1. a kind of wide-range high-precision pipettor, it is characterised in that:Including piston (1), dialing knob (2), piston cylinder (3), move
Liquid interface (4);Piston (1) is made of spiral handspike and square bodily form teflon piston head, and piston head is interior with piston cylinder (3)
Chamber linking sealing;Piston cylinder (3) the intracavity section square, piston cylinder (3) front have volume scale, minimum indexing 0.5
Milliliter;The dialing knob (2) is made of symmetrical two parts, is screwed, and periphery has 50 equi-distance scale lines, most
Small indexing is 10 microlitres;There are two the different interfaces of diameter for liquid relief interface (4) tool.
2. a kind of wide-range high-precision pipettor according to claim 1, it is characterised in that:Piston cylinder (3) intracavity section
The square length of side is 1 centimetre, and liquid relief range is accurate adjustable from 10 microlitres to 10 milliliter.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810833260.5A CN108745433A (en) | 2018-07-23 | 2018-07-23 | A kind of wide-range high-precision pipettor |
Applications Claiming Priority (1)
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CN201810833260.5A CN108745433A (en) | 2018-07-23 | 2018-07-23 | A kind of wide-range high-precision pipettor |
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CN108745433A true CN108745433A (en) | 2018-11-06 |
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CN201810833260.5A Pending CN108745433A (en) | 2018-07-23 | 2018-07-23 | A kind of wide-range high-precision pipettor |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110498135A (en) * | 2019-08-01 | 2019-11-26 | 广东和翔制药有限公司 | A kind of spiral pushing device |
CN110849673A (en) * | 2019-12-23 | 2020-02-28 | 陈黎黎 | Quantitative sampling device for medical examination |
CN111101193A (en) * | 2020-01-10 | 2020-05-05 | 贵阳航发精密铸造有限公司 | Casting crystal-leading segment forming die capable of accurately controlling crystal orientation growth of single crystal blade |
-
2018
- 2018-07-23 CN CN201810833260.5A patent/CN108745433A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110498135A (en) * | 2019-08-01 | 2019-11-26 | 广东和翔制药有限公司 | A kind of spiral pushing device |
CN110849673A (en) * | 2019-12-23 | 2020-02-28 | 陈黎黎 | Quantitative sampling device for medical examination |
CN111101193A (en) * | 2020-01-10 | 2020-05-05 | 贵阳航发精密铸造有限公司 | Casting crystal-leading segment forming die capable of accurately controlling crystal orientation growth of single crystal blade |
CN111101193B (en) * | 2020-01-10 | 2022-01-28 | 贵阳航发精密铸造有限公司 | Casting crystal-leading segment forming die capable of accurately controlling crystal orientation growth of single crystal blade |
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Addressee: Wang Shunbin Document name: Deemed withdrawal notice |
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Application publication date: 20181106 |
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