CN208140729U - A kind of trace liquid quantitative sampler - Google Patents
A kind of trace liquid quantitative sampler Download PDFInfo
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- CN208140729U CN208140729U CN201820492429.0U CN201820492429U CN208140729U CN 208140729 U CN208140729 U CN 208140729U CN 201820492429 U CN201820492429 U CN 201820492429U CN 208140729 U CN208140729 U CN 208140729U
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Abstract
The utility model discloses a kind of trace liquid quantitative sampler, the trace liquid quantitative sampler includes substrate；Liquid inflow port, sampling pipe, quantitative sampling chamber, spill cavity and the gas vent being sequentially communicated are equipped in the substrate；The quantitative sampling chamber is connected to liquid flowing outlet；The inner wall of the liquid flowing outlet is equipped with nano-hydrophilic material layer, lets the liquid out mouth as liquid discharge tube road and control valve.The utility model guarantees the accuracy and repeatability of every sub-sampling by micro-structure, and does not have to additional setting control valve.
Description
Technical field
The utility model relates to a kind of trace liquid quantitative samplers, belong to micro（10—50uL）Liquid quantitative sampling
Field.
Background technique
Quantitative draw liquid is frequently necessary in medical test experiment, and filling liquid, this process are all by special implement again
What manual operation was completed.There are two types of common dedicated micro quantitative determination sampler tools：First is that micropipette rifle.Micropipette rifle is to move
One kind of liquid device, pipettor have different specifications, and liquid relief range of capacity 0.1ul -200ul can set knob by range and set
Determine sampling amount, the liquid-transfering gun of different size matches different size of fluid suction head.Second is that micro-capillary tubes.Micro-capillary tubes are more
For glass tube, scale is indicated on outer tube wall, quantitatively micro liquid can be drawn with the use of rubber pneumatic bag, take liquid range of capacity
0.1ml—50ml.Above two micro liquid sampler is suitble to use in laboratory, and operation is strongly professional, is only suitable for professional people
Member uses.
In medical inspection instrument, microsampling sample-adding is also used.Its technical thought realized is substantially derived from micro-
Liquid-transfering gun is measured, structure can be summarized as：Micropump+control valve.Micropump includes microlaser cavity and piston, and piston is in microlaser cavity
Movement, it is assumed that the stroke of piston is L, and the sectional area of miniature cavities is S, then the capacity V for sampling liquid is V=L*S.Micropump can
It with there are many kinds of structure changes, but all be unable to do without and realizes sampling based on above-mentioned sampling theorem, that is, pass through control piston motion
Stroke determines sampling capicity.Control valve is used to control the flow direction of sampling liquid, is often triple valve, draw when sample liquid to be sampled with
Cavity connection, piston motion form negative pressure, draw liquid.Draw sample movement after, control valve events, shutdown cavity with it is to be sampled
Connection between liquid, opens the connection between cavity and drain line, and piston reverses direction movement is discharged liquid, it is dynamic to complete sample-adding
Make.In many testing instruments, in order to reduce cross contamination, control valve is not used, sample cell and reaction tank are independently arranged, taken
After sample head draws sample from sample cell, reaction tank position, then drain are moved to sample head is drawn.This method and the manually original of sampling sample-adding
Reason be it is identical, only with stepper motor+driving structure instead of manually drawing and arranging, with the step number of stepper motor instead of quarter
Degree.
In addition, some related patents devise several money micro quantitative determination samplers, after study, these Patent designs
Device is all the accuracy that ensure that sampling by structure design, reduces the requirement to operator, but all or with artificial hand
It is still manual trace sampling apparatus premised on dynamic operation.
Patent in terms of the quantitative sampling that can be retrieved at present is all based on above-mentioned sampling sample-adding principle, the feature of sampling
There is two o'clock：One is the need for moving component to complete to draw sample, such as piston；Second is that being taken by the stroke for controlling movement parts to control
The capacity of sample；Sample-adding is characterized in：It draws sample position and sample-adding position is separately positioned.Be draw sample move back sample-adding position be loaded again,
It is increased by control valve, sample-adding is completed by the flow direction that control valve changes liquid.
For example Chinese patent CN204043955U discloses a kind of quantitative sampling device comprising quantitative sampling chamber, piston
And capillary, quantitative sampling chamber do not play quantitative effect.Its quantitative sampling principle is：Push piston in piston channel by manpower
It is mobile, since piston channel is connected to quantitative sampling chamber, quantitative sampling chamber is caused to form negative pressure.Under the action of negative pressure, sample
It can be intracavitary by capillary suction quantitative sampling.The dosage of sampling is according to the mobile stroke of the size and piston of piston come really
It is fixed, once the size of piston determines, the capacity of quantitative sampling just is controlled by controlling the stroke of piston.It describes, determines accordingly
Amount sampling theorem is not different with conventional method above-mentioned or device.
Utility model content
The utility model is intended to provide a kind of trace liquid quantitative sampler, which guarantees often by micro-structure
The accuracy and repeatability of sub-sampling, and do not have to additional setting control valve.
To achieve the goals above, technical solution used by the utility model is：
A kind of trace liquid quantitative sampler, including substrate；It is structurally characterized in that, is equipped with and is sequentially communicated in the substrate
Liquid inflow port, sampling pipe, quantitative sampling chamber, spill cavity and gas vent；The quantitative sampling chamber and liquid flowing outlet connect
It is logical；The inner wall of the liquid flowing outlet is equipped with nano-hydrophilic material layer, lets the liquid out mouth as liquid discharge tube road and control
Valve.
When liquid flows into quantitative sampling chamber, the capillarity and nano-hydrophilic of the liquid flowing outlet pipeline of the utility model
The resistance that material layer generates makes liquid holdup intracavitary in quantitative sampling, is equivalent to valve closing.When external pressure is greater than pipeline
After the resistance of generation（When needing to be loaded）, liquid is equivalent to valve opening from quantitative sampling chamber flow ipe.
By above structure, the novel place of the sampling sample-adding principle of the utility model is：
First is that by the fixed volume of micro-structure come quantitative sampling, rather than quantified by movement travel；
Sample is drawn second is that realizing by the flowing of liquid, rather than sample is drawn by the movement of movement parts；
Third is that realizing the function of control valve by micro-structure and process, do not need to move, is able to achieve after drawing sample and just adds
Sample.
The utility model is based on fluid mechanics principle design sample and is loaded pipeline, realizes quantitative by structure design
Sample substitutes miniature valve by structure and technique, solves the problems, such as biochip quantitative sampling, so that many inspections of biochip
Survey project is from qualitative or sxemiquantitative leap to quantitative detection.
Embodiment according to the present utility model can also make further optimization to the utility model, and the following are after optimization
The technical solution of formation：
In order to which the function that liquid flowing outlet doubles as liquid discharge tube road and control valve, the pipe of liquid flowing outlet is better achieved
Road size d is less than 500um.
Preferably, the substrate is mainly by one piece of egative film and one piece of cover plate through being bonded；The cover plate is one flat plate,
Entire pipeline is machined on the egative film.
Preferably, the size of the substrate is as follows：Long L≤50mm, height H≤20mm, thick D≤8mm.
Preferably, the diameter of the sampling pipe is less than 1mm.
Preferably, the quantitative sampling chamber uses rectangular parallelepiped structure, and sampling capicity is 10-50uL.It is preferred that quantitative sampling chamber
Bottom have chamfering.
Preferably, the length dimension of the quantitative sampling chamber be at least quantitative sampling chamber height or 5 times of thickness
More than.
Preferably, the inner wall of the quantitative sampling chamber smears nano-sized hydrophobic material layer.
Preferably, intracavitary be equipped with from the upper end of substrate of the quantitative sampling is packed into sample-adding piston, is equipped on the sample-adding piston
Sealing ring, sample-adding piston are used to be connected by piston rod with external drive part.
The utility model is particularly suitable for microminiature device as such as biochip, and biochip is exactly one miniature
Biology laboratory, bioprocesses are integrated in biochip and complete, and the shape three-dimensional dimension of biochip generally all only has
Several millimeters, internal liquid line size is generally all within 1000um.Therefore, conventional quantitative sampling loading methods are just
It is completely not applicable, because having no room to integrate the micropump or miniature valve of routine.
Compared with prior art, the utility model has the beneficial effects that：
1, the utility model is suitable for microsampling, and sampling amount range is sampling precision 3% within 10-50uL.
2, guarantee the accuracy and repeatability of every sub-sampling by micro-structure；It is filled using the small size that the utility model designs
It sets and can be achieved with micro quantitative determination sampling, not only sampling is accurate, but also the processing is simple, feasible process, can be used as an important portion
Part is widely used in the design and manufacture of biochip.
3, reasonable structure size is designed using hydrodynamics method, then uses process of surface treatment, realized with structure
The function of control valve avoids instead of essential control valve in routine sampling device and installs valve in small device sizes
Problem.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of existing sampler；
Fig. 2 is the structure principle chart of the utility model one embodiment；
Fig. 3 is A-A cross-sectional view of Fig. 2.
In figure
1- substrate；2 liquid inflow ports；3- quantitative sampling chamber；4- liquid flowing outlet；5- spill cavity；6- gas vent；7- sample-adding
Piston；8- sampling pipe；101-quantitative sampling chambers, 102-capillaries, 103-plugs, 104-pistons, 105-piston rods,
106-piston channels.
Specific embodiment
The utility model is described in detail below with reference to attached drawing and in conjunction with the embodiments.It should be noted that not conflicting
In the case where, the feature in the embodiments of the present invention and embodiment can be combined with each other.For sake of convenience, hereinafter such as
There is "upper", "lower", "left", "right" printed words, only indicates consistent with the upper and lower, left and right direction of attached drawing itself, not to structure
Play restriction effect.
A kind of trace liquid quantitative sampler, using quantitative cavity as the measurer of quantitative sampling；Utilize liquid line
Complete sampling action；Utilize the flowing of cleverly fluid circuit structure and technology controlling and process liquid；Sample-adding is completed using sample-adding piston
Movement.
A kind of quantitative sampling sample adding device designed based on these technical thoughts is as shown in Figures 2 and 3, sampler base
Piece 1 be entire sampler carrying basis, it by one piece of egative film and one piece of cover plate through being bonded, outer dimension：Long L≤
50mm, height H≤20mm, thick D≤8mm.Cover plate is exactly one flat plate, and entire pipeline, including liquid flow are machined on egative film
Entrance 2, quantitative sampling chamber 3, liquid flowing outlet 4, spill cavity 5, gas vent 6 and sampling pipe 8.All pipeline rulers in device
Very little both less than 1mm, the sampling capicity of sampler are 10-50uL.
Quantitative sampling chamber 3 uses rectangular parallelepiped structure, and the size of quantitative sampling chamber is determined according to sampling volume, length dimension
It is more much bigger than height and thickness, it is long/high（It is thick）Than being greater than 5, and chamfering is wanted in bottom, and the inner wall of quantitative sampling chamber smears nanometer
Hydrophobic material.
The line size d of liquid flowing outlet 4 is other than being liquid discharge tube road, and there are also the functions of control valve.Line size d
It is less than 500um.At the liquid flowing outlet of the bottom of quantitative sampling chamber, need to smear water wetted material.When liquid flows into quantitatively
When sampling cavity, the resistance that line size d and nano surface water wetted material generate jointly, so that liquid holdup is in quantitative sampling chamber
It is interior, it is equivalent to valve closing.After external pressure is greater than the resistance that pipeline generates, liquid is flowed out from quantitative sampling chamber influent
Mouth 4 is equivalent to valve opening.
It is loaded piston 7 to be packed into from the upper end of substrate, sealing ring is housed on piston, be tightly connected, external drive part acts on
Piston can be driven at the top of sample-adding piston rod.
Sampling method is as follows：
Sampling pipe 8 is added from liquid inflow port 2 in liquid, and liquid, which can be, to be manually added and can also be infused by front ring section structure
Enter.After liquid enters device, quantitative sampling chamber 3 is first flowed into along sampling channel.After equal quantitative samplings chamber is filled, liquid overflows fixed again
It measures sampling cavity and flows into spill cavity 5 along pipeline.Due to being reserved with air outlet 6, the liquid that the air in pipeline is poured into is squeezed out
Pipeline, liquid can fill quantitative sampling chamber 3.After liquid fills quantitative sampling chamber, extra liquid just continues along sampling pipe
It flows forward, and pours into spill cavity 5.Extra liquid will be in sampling pipe and spill cavity after being stored in quantitative sampling chamber.
The range that total amount V is added in liquid is controlled, suitable total amount V should be：Quantitative sampling chamber capacity≤V≤quantitative sampling chamber capacity+overflow
Chamber capacity out.In sampling process, sample-adding piston be in that initial position is fixed, and it is true that the initial position of piston passes through position location
It is fixed.
Loading methods are as follows：
In sampling process, liquid is first filled with quantitative sampling chamber, and surplus liquid flows into spill cavity again.In entire sampling process
In, quantitative sampling is intracavitary hydraulically full always.When needing to be loaded, outer power drive piston moves down, and it is intracavitary to squeeze quantitative sampling
Liquid all flow into sample-adding pipeline, then from liquid flowing outlet 4 flow out, complete sample-adding movement.
The content that above-described embodiment illustrates should be understood as that these embodiments are only used for being illustrated more clearly that the utility model,
Rather than limitation the scope of the utility model, after having read the utility model, those skilled in the art are practical new to this
The modification of the various equivalent forms of type each falls within the application range as defined in the appended claims.
Claims (10)
1. a kind of trace liquid quantitative sampler, including substrate（1）；It is characterized in that, the substrate（1）Interior be equipped with successively connects
Logical liquid inflow port（2）, sampling pipe（8）, quantitative sampling chamber（3）, spill cavity（5）And gas vent（6）；The quantitative sampling
Chamber（3）With liquid flowing outlet（4）Connection；The liquid flowing outlet（4）Inner wall be equipped with nano-hydrophilic material layer, let the liquid out
Mouthful（4）As liquid discharge tube road and control valve.
2. trace liquid quantitative sampler according to claim 1, which is characterized in that liquid flowing outlet（4）Pipeline
Size d is less than 500um.
3. trace liquid quantitative sampler according to claim 1, which is characterized in that the substrate（1）Mainly by one
Block egative film and one piece of cover plate are through being bonded；The cover plate is one flat plate, is machined with entire pipeline on the egative film.
4. trace liquid quantitative sampler according to claim 1, which is characterized in that the substrate（1）Size such as
Under：Long L≤50mm, height H≤20mm, thick D≤8mm.
5. trace liquid quantitative sampler according to claim 1, which is characterized in that the sampling pipe（8）It is straight
Diameter is less than 1mm.
6. trace liquid quantitative sampler according to claim 1, which is characterized in that the quantitative sampling chamber（3）It adopts
With rectangular parallelepiped structure, sampling capicity is 10-50uL.
7. trace liquid quantitative sampler according to claim 1, which is characterized in that the quantitative sampling chamber（3）'s
Bottom has chamfering.
8. trace liquid quantitative sampler according to claim 1, which is characterized in that the quantitative sampling chamber（3）'s
Length dimension be at least quantitative sampling chamber height or 5 times or more of thickness.
9. trace liquid quantitative sampler according to claim 1, which is characterized in that the quantitative sampling chamber（3）'s
Inner wall smears nano-sized hydrophobic material layer.
10. trace liquid quantitative sampler according to any one of claims 1-5, which is characterized in that described quantitative
Sampling cavity（3）It is interior to be equipped with from substrate（1）Upper end be packed into sample-adding piston（7）, the sample-adding piston（7）It is upper that sealing ring, sample-adding are housed
Piston（7）For being connected by piston rod with external drive part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820492429.0U CN208140729U (en) | 2018-04-09 | 2018-04-09 | A kind of trace liquid quantitative sampler |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820492429.0U CN208140729U (en) | 2018-04-09 | 2018-04-09 | A kind of trace liquid quantitative sampler |
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| CN208140729U true CN208140729U (en) | 2018-11-23 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108535504A (en) * | 2018-04-09 | 2018-09-14 | 湖南乐准智芯生物科技有限公司 | A kind of trace liquid quantitative sampler and sampling loading methods |
| CN108535504B (en) * | 2018-04-09 | 2024-06-11 | 湖南乐准智芯生物科技有限公司 | Quantitative sampling device and sampling and adding method for trace liquid |
-
2018
- 2018-04-09 CN CN201820492429.0U patent/CN208140729U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108535504A (en) * | 2018-04-09 | 2018-09-14 | 湖南乐准智芯生物科技有限公司 | A kind of trace liquid quantitative sampler and sampling loading methods |
| CN108535504B (en) * | 2018-04-09 | 2024-06-11 | 湖南乐准智芯生物科技有限公司 | Quantitative sampling device and sampling and adding method for trace liquid |
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