CN108721437A - 一种茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用 - Google Patents
一种茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用 Download PDFInfo
- Publication number
- CN108721437A CN108721437A CN201810770975.0A CN201810770975A CN108721437A CN 108721437 A CN108721437 A CN 108721437A CN 201810770975 A CN201810770975 A CN 201810770975A CN 108721437 A CN108721437 A CN 108721437A
- Authority
- CN
- China
- Prior art keywords
- mir
- inhibitor
- rice
- blood pressure
- tea seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091079007 miR-376b stem-loop Proteins 0.000 title claims abstract description 27
- 206010020772 Hypertension Diseases 0.000 title claims abstract description 14
- 239000003112 inhibitor Substances 0.000 title claims abstract description 10
- 230000001225 therapeutic effect Effects 0.000 title abstract description 4
- 241001122767 Theaceae Species 0.000 title abstract 2
- 241001596328 Camellia meiocarpa Species 0.000 claims abstract description 21
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 16
- 235000009566 rice Nutrition 0.000 claims abstract description 16
- 239000003814 drug Substances 0.000 claims abstract description 11
- 229940079593 drug Drugs 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 9
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 6
- 230000002265 prevention Effects 0.000 claims abstract description 5
- 241000209094 Oryza Species 0.000 claims description 14
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 2
- 241000700159 Rattus Species 0.000 abstract description 14
- 230000036772 blood pressure Effects 0.000 abstract description 14
- 230000014509 gene expression Effects 0.000 abstract description 8
- AIGAZQPHXLWMOJ-UHFFFAOYSA-N Tanshinone I Chemical compound C1=CC2=C(C)C=CC=C2C(C(=O)C2=O)=C1C1=C2C(C)=CO1 AIGAZQPHXLWMOJ-UHFFFAOYSA-N 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 230000001435 haemodynamic effect Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 229930183118 Tanshinone Natural products 0.000 abstract description 3
- 230000001631 hypertensive effect Effects 0.000 abstract description 3
- 230000002159 abnormal effect Effects 0.000 abstract description 2
- 238000007634 remodeling Methods 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract 2
- 230000029865 regulation of blood pressure Effects 0.000 abstract 2
- 230000017531 blood circulation Effects 0.000 abstract 1
- 238000011160 research Methods 0.000 abstract 1
- 244000269722 Thea sinensis Species 0.000 description 9
- 210000004165 myocardium Anatomy 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000209507 Camellia Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 101000836077 Rattus norvegicus Prostatic spermine-binding protein Proteins 0.000 description 1
- 101000703768 Rattus norvegicus Sex hormone-binding globulin Proteins 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 235000018597 common camellia Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000035487 diastolic blood pressure Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035488 systolic blood pressure Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- HFFLGKNGCAIQMO-UHFFFAOYSA-N trichloroacetaldehyde Chemical compound ClC(Cl)(Cl)C=O HFFLGKNGCAIQMO-UHFFFAOYSA-N 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明公开了一种茶粕用作miR‑376b‑5p抑制剂及在高血压治疗方面的应用。本发明发现，小果油茶粕为miR‑376b‑5p的有效抑制剂。研究显示，miR‑376b‑5p对丹参酮改善老年SHR血压及血流动力学的作用有逆转效果，由于较高的基础血压和异常血流动力学状况可影响心室重构的进程，miR‑376b‑5p可能通过影响血压及血流动力学参与对丹参酮作用的调控。本发明中，小果油茶粕通过抑制miR‑376b‑5p的表达显著降低高血压模型大鼠的血压水平，可以制备成防治高血压的食品、药品或保健品。
Description
技术领域
本发明涉及小果油茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用。
背景技术
小果油茶(Camellia meiocarpa Hu.)又名江西子、小茶、鸡心子、小叶油茶、羊屎子等。果小、叶小、芽小，芽、苞片没有毛是其显著特征。小果油茶耐荫、果小、皮薄、产量高，其栽培面积和年产量仅次于普通油茶，分布于福建、江西、广西、湖南、贵州、江浙等省。
小果油茶所含化学成分主要有皂苷、黄酮、多酚、多糖和蛋白类化合物等。药理研究表明，油茶植物不仅具有抗菌、消炎、清热解毒等功效，而且在抗氧化、抗肿瘤等方面也显示出较好的生物活性，其中油茶皂苷对心脑血管的药理活性显著。
发明内容
本发明目的在于提供小果油茶粕用作miR-376b-5p抑制剂及治疗高血压的应用。
本发明的上述目的是通过下面的技术方案得以实现的：
小果油茶粕用作miR-376b-5p抑制剂的用途。
小果油茶粕用于制备防治高血压的食品、药品或保健品的用途。
一种用于抑制miR-376b-5p表达的食品、药品或保健品，含有小果油茶粕，还含有食品、药品或保健品中可以接受的载体或赋形剂。
本发明发现，小果油茶粕为miR-376b-5p的有效抑制剂。研究显示，miR-376b-5p对丹参酮改善老年SHR血压及血流动力学的作用有逆转效果，由于较高的基础血压和异常血流动力学状况可影响心室重构的进程，miR-376b-5p可能通过影响血压及血流动力学参与对丹参酮作用的调控(参考文献：丹参酮通过抑制miR-376b-5p逆转自发性高血压大鼠左室重构，中国老年学杂志2016年11月第36卷)。本发明中，小果油茶粕通过抑制miR-376b-5p的表达显著降低高血压模型大鼠的血压水平，可以制备成防治高血压的食品、药品或保健品。
具体实施方式
下面结合实施例具体介绍本发明实质性内容，但并不以此限定本发明的保护范围。
一、实验材料
取小果油茶的茶果洗净、风干后压榨除油制备得到小果油茶粕。
SPF级9周龄SHR，，体质量180～200g，由北京维通利华实验动物技术有限公司提供。
二、实验方法
1、分组和给药
所有大鼠自由饮食饲养3d后，均适应性测量血压5次。平均血压≥160mmHg(1mmHg＝0.133kPa)的SHR进入实验分组，称体质量后按随机数字表法分为模型组(蒸馏水，1mL/100g)和茶粕低、中、高剂量组(小果油茶粕50、100、200mg/kg)，每组8只。每7天称量一次体重调整给药量，各组大鼠每天灌胃给予相应药物1次，连续给药28d。
2、血压测定
最后一次给药6h后，将各组大鼠置于配套固定器中，温水浸泡软化尾巴，采用PowerLab ML125/R无创尾动脉血压测定分析系统测定大鼠血压。重复3次，取均值。
3、miR-376b-5p表达水平测定
最后一次给药后将各组大鼠禁食、不禁水12h，按照其体质量不同分别采用3％水合氯醛腹腔注射，麻醉后颈椎脱臼处死，开胸取出心脏。
取各组大鼠心肌组织50mg，DEPC冲洗后，按Trizol一步法提取总RNA，紫外分光光度计测其纯度与浓度，其中OD260/OD280在1.8～2.0代表其纯度较好。提取总RNA后，采用颈环结构的MegaPlexTM RNA反转录引物混合物进行反转录，反转录结束后按照miRNA RT-PCR反应体系进行扩增，以U6作为内参照，进行PCR产物的半定量分析。引物序列为：
U6上游引物：5'-GCCCGATCTTGTCTGATCTC-3'；
U6下游引物：5'-CCTGACCCTGCTTAGCTTCC-3'；
miR-376b-5p上游引物：5'-GTAATAGATACTGATCGTCTAGTC-3'；
miR-376b-5p下游引物：5'-CTCAGTCATAACGTCTGATATTGT-3'。
PCR反应条件：95℃预变性10min，95℃变性15s，60℃退火30s，75℃延伸30s，75℃终极延伸1min，共40个循环。采用2-ΔΔCt法计算miR-376b-5p的相对表达水平。
4、数据处理及分析
采用SPSS19.0软件，多组间比较采用单因素方差分析检验，组间两两比较采用q检验。以P＜0.05为差异有统计学意义。
三、实验结果
1、各组大鼠血压水平比较
与模型组相比，茶粕低、中、高剂量组大鼠收缩压(SBP)和舒张压(DBP)均显著降低(P＜0.05)，且SBP、DBP降低程度与茶粕给药剂量呈现一定依赖性。结果见表1。
表1各组大鼠SBP、DBP测定结果比较
2、各组大鼠心肌miR-376b-5p表达水平比较结果
与模型组相比，茶粕低、中、高剂量组大鼠心肌miR-376b-5p表达水平显著降低(P＜0.05)，且降低程度与茶粕给药剂量呈现一定依赖性。结果见表2。
表2各组大鼠心肌miR-376b-5p表达水平比较
上述结果表明，小果油茶粕通过抑制miR-376b-5p的表达显著降低高血压模型大鼠的血压水平，可以制备成防治高血压的食品、药品或保健品。另外，小果油茶粕还可以用作筛选其他miR-376b-5p抑制剂的阳性药。
上述实施例的作用在于具体介绍本发明的实质性内容，但本领域技术人员应当知道，不应将本发明的保护范围局限于该具体实施例。
Claims (3)
1.小果油茶粕用作miR-376b-5p抑制剂的用途。
2.小果油茶粕用于制备防治高血压的食品、药品或保健品的用途。
3.一种用于抑制miR-376b-5p表达的食品、药品或保健品，其特征在于：含有小果油茶粕，还含有食品、药品或保健品中可以接受的载体或赋形剂。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810770975.0A CN108721437A (zh) | 2018-07-13 | 2018-07-13 | 一种茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810770975.0A CN108721437A (zh) | 2018-07-13 | 2018-07-13 | 一种茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108721437A true CN108721437A (zh) | 2018-11-02 |
Family
ID=63926369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810770975.0A Pending CN108721437A (zh) | 2018-07-13 | 2018-07-13 | 一种茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108721437A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402258A (zh) * | 2018-11-09 | 2019-03-01 | 山东大学第二医院 | miR-376b-5p在制备高级别胶质瘤和颅内淋巴瘤诊断和鉴别诊断制剂中的应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181027A (zh) * | 2007-12-13 | 2008-05-21 | 黄宝生 | 从油茶籽粕中提取茶多糖的方法 |
| CN102276679A (zh) * | 2011-06-27 | 2011-12-14 | 中国林业科学研究院林产化学工业研究所 | 一种从油茶饼粕减压沸腾提取高纯度茶皂素的方法 |
| WO2018017865A1 (en) * | 2016-07-20 | 2018-01-25 | Thomas Jefferson University | Inhibiting microrna to prevent development of essential hypertension |
-
2018
- 2018-07-13 CN CN201810770975.0A patent/CN108721437A/zh active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181027A (zh) * | 2007-12-13 | 2008-05-21 | 黄宝生 | 从油茶籽粕中提取茶多糖的方法 |
| CN102276679A (zh) * | 2011-06-27 | 2011-12-14 | 中国林业科学研究院林产化学工业研究所 | 一种从油茶饼粕减压沸腾提取高纯度茶皂素的方法 |
| WO2018017865A1 (en) * | 2016-07-20 | 2018-01-25 | Thomas Jefferson University | Inhibiting microrna to prevent development of essential hypertension |
Non-Patent Citations (6)
| Title |
|---|
| 中国法制出版社: "《中华人民共和国食品安全法 实用版》", 31 May 2015, 中国法制出版社 * |
| 季昆森: "《壮大循环经济 推进创业就业文集》", 31 August 2009, 安徽人民出版社 * |
| 罗扬: "《贵州油茶》", 30 June 2014, 贵州科技出版社 * |
| 谢宗万: "《中药品种理论研究》", 30 April 1991, 中国医药出版社 * |
| 郭显荣等: "植物源ACE抑制肽研究进展 ", 《经济林研究》 * |
| 陈强等: ""丹参酮通过抑制miR-376b-5p逆转自发性高血压大鼠左室重构"", 《中国老年学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402258A (zh) * | 2018-11-09 | 2019-03-01 | 山东大学第二医院 | miR-376b-5p在制备高级别胶质瘤和颅内淋巴瘤诊断和鉴别诊断制剂中的应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mayell | Maitake extracts and their therapeutic potential-a review | |
| KR101074158B1 (ko) | 간 보호 또는 간 질환의 예방 및 치료용 인삼다당체 조성물 | |
| US8927008B2 (en) | Compound sea cucumber product, preparation method, and dosage forms thereof | |
| CN103349711B (zh) | 含有白藜芦醇的抗衰老组合物及其制备方法 | |
| CN104305453A (zh) | 一种西洋参复合抗疲劳运动保健饮品 | |
| KR20070100560A (ko) | 면역증강을 위한 기능성 음료 및 그 제조방법 | |
| CN108721437A (zh) | 一种茶粕用作miR-376b-5p抑制剂及在高血压治疗方面的应用 | |
| WO2013059942A1 (en) | Use of a ginseng extract to prevent and treat alopecia areata | |
| TWI678211B (zh) | 管花肉蓯蓉萃取物及異類葉升麻苷於保護肌肉之用途 | |
| KR101765246B1 (ko) | 갱년기 관련 질환 또는 증상의 예방 및 개선용 조성물 | |
| Mishra et al. | Cordicepssinensis: the Chinese Rasayan-current research scenario | |
| CN104921109A (zh) | 一种抗衰老保健品及其检测方法 | |
| KR101865142B1 (ko) | 조팝나무, 배나무, 뱀무의 혼합 추출물을 유효성분으로 함유하는 알러지성 질환의 예방 및 치료용 약학적 조성물 | |
| CN104547523A (zh) | 一种增强免疫力的药物及其制备方法 | |
| CN107233488A (zh) | 一种延缓细胞及血管衰老的中药组合物 | |
| KR101479096B1 (ko) | 생약혼합물의 추출물을 함유하는 산전산후부종 예방 또는 개선용 건강기능식품 | |
| CN109464645B (zh) | 一种健脾养胃抗衰老膏滋及其制备方法和应用 | |
| KR20140146378A (ko) | 생약혼합물을 유효성분으로 함유하는 피로회복용 조성물 | |
| CN104306934B (zh) | 一种具有增强免疫力的药物组合物及其制备方法 | |
| CN105168574A (zh) | 一种玛咖保健口服液及其制备方法 | |
| CN101244047B (zh) | 二十八烷醇在制备防治高原红细胞增多症药物中的应用 | |
| KR20190075889A (ko) | 땃두릅나무 추출물을 유효성분으로 함유하는 관절염의 예방 또는 치료용 약학적 조성물 | |
| CN105727230B (zh) | 一种治疗皮肤病的玫瑰中药组合物及其制备方法和应用 | |
| CN105456102A (zh) | 一种含有红景天提取物、何首乌提取物和枸杞提取物的美白化妆品 | |
| CN104825709A (zh) | 一种治疗寄生虫病的中药组合物 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181102 |