实施例6MYORG致病基因检验
基于本发明揭示的PFBC致病基因MYORG的9种致病突变基因形式,本发明进一步提供了所述MYORG基因突变的检测试剂盒和检测方法,并应用于对6个PFBC家系中的个体进行了MYORG基因突变检测,具体如下:
(1)标本采集与处理
对PFBC家系A-F患者及相关成员进行详细的病史和家族史调查,以及规范的神经系统体格检查和相关辅助检查信息,在其签署了知情同意书的情况下,抽取肘静脉血5ml(枸橼酸钠抗凝),提取基因组DNA。
(2)MYORG突变基因片段扩增和测序
以(1)中基因组DNA为模板,采用本发明试剂盒对MYORG基因片段进行PCR体外扩增。本发明的扩增试剂盒主要组分包括日本Takara公司的rTaq聚合酶(TaKaRa,商品编码为DR100A)以及该rTaq聚合酶配套的10×PCR buffer、dNTP Mix,以及引物组合SED ID NO:21-26(引物由上海生工生物工程有限公司合成)。扩增体系详见表2,其中引物对F和R的选择参见表3,扩增条件为:94℃预变性3min;30个循环:94℃变性30s,60℃退火30s,72℃延伸30s;72℃终延伸5min。
表2扩增体系
编号 |
组份 |
添加量/μL |
1 |
10×PCR buffer(Mg2+) |
2.5 |
2 |
dNTP Mix |
0.5 |
3 |
Primer F(50μM) |
0.25 |
4 |
Primer R(50μM) |
0.25 |
5 |
rTaq DNA聚合酶 |
0.25 |
6 |
基因组DNA模板(30ng/μL) |
1.0 |
7 |
灭菌ddH2O |
补足至20 |
(3)MYORG基因突变分析
将(2)中PCR产物送上海生工生物工程股份有限公司进行Sanger测序,分析测序结果,找出突变位点。
表3扩增引物对
结果(表3)显示A-F这6个家系均含有复合杂合或纯合突变。家系A的所有患者都具有SED ID NO:2所示突变,家系B的所有患者都具有SED ID NO:3和4所示突变,家系C的所有患者都具有SED ID NO:2和5所示突变,家系D的所有患者都具有SED ID NO:6所示突变,家系E的所有患者都具有SED ID NO:7和8所示突变,家系F的所有患者都具有SED ID NO:9和10所示突变。而各家系内正常人个体和1000名正常对照(实施例5)都没有上述突变。证明本发明提供的方法能够精确检测和分析常染色体隐性遗传PFBC。
虽然本发明是通过优选的具体实施例进行说明,但是对于本领域的技术人员来说,本发明可以有各种更改和变化。凡在本发明的精神和原则之内,所做的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。
序列表
<110> 福建医科大学附属第一医院
中国科学院上海生命科学研究院
<120> 原发性家族性脑钙化症致病基因MYORG及其应用
<160> 28
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 1
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 2
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 2
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctgatgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 3
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 3
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactagt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 4
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 4
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctgtgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 5
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 5
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
ggcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 6
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 6
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tctttgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 7
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 7
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggctagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 8
<211> 2145
<212> DNA
<213> 智人(Homo sapiens)
<400> 8
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
ctttaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg acttcgatga ggtcaaattc cccaacgcca gcgacatgtt ccgccgcctg 1140
cgcgacgccg gcttccgcgt cacgctctgg gtgcaccctt ttgtcaacta caactcgtcg 1200
cgcttcggcg agggcgtgga gcgcgagctg ttcgtgcgcg aacccacggg ccggttacct 1260
gcgctggtgc gctggtggaa cggcatcggc gcggtgctag acttcacgca cccaaaggcc 1320
cgcgactggt tccagggaca cctgcggcgg ctgcgctctc gctactccgt ggcttccttc 1380
aagttcgacg cgggcgaggt cagctacctg ccgcgggact tcagcaccta ccggccgctg 1440
ccggacccca gcgtctggag ccggcgctac actgagatgg cgctgccctt cttctcgctg 1500
gcggaggtgc gcgtaggcta ccagtcacag aacatctcct gcttcttccg cctggtggat 1560
cgcgactctg tgtggggcta cgacctgggg ttgcgctcac tcatccccgc ggtgctcacc 1620
gtcagcatgc tgggctaccc attcatccta cccgatatgg tgggcggcaa cgccgtgccc 1680
cagcggacag ccggcggcga tgtgcccgag cgcgagctct acattcgctg gctggaagtg 1740
gccgccttta tgccggccat gcagttctct atcccgccct ggcgctacga cgcggaagtg 1800
gtggccatcg cgcagaagtt cgccgccctg cgggcctcgc ttgtggcacc gctgttgctt 1860
gagctggcgg gcgaggtcac cgacacgggt gaccctatcg tgcgccccct ttggtggatt 1920
gcgcccggcg acgagacagc tcaccgtatc gactcgcagt tccttattgg ggacacgctg 1980
cttgtggccc cggtgctgga gccaggcaag caggagcgcg acgtctattt gcccgccggc 2040
aagtggcgca gctacaaggg tgagcttttc gacaagacgc cggtgctgct caccgattac 2100
ccggtcgacc tggatgagat cgcctacttt acctgggcgt cctga 2145
<210> 9
<211> 2157
<212> DNA
<213> 智人(Homo sapiens)
<400> 9
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgcct ggccttccgc 360
tccggcgcgc tggaccttga ctcctgcagc cgcgatggcg ccctgctggg ctgctcgctc 420
acggccgacg ggctgccgct gcacttcttc atccagactg tgcggcccaa ggacacggtc 480
atgtgctacc gcgtgcgctg ggaggaggca gcgccgggcc gggccgtgga gcacgccatg 540
ttcttgggcg acgcggcggc ccactggtat ggtggcgccg agatgaggac gcaacactgg 600
cccatccgcc tggatggcca gcaggagccc cagccgttcg tcaccagcga tgtctactcc 660
tccgacgccg cgtttggggg catcctcgag cgctactggc tatcttcgcg cgcggccgcc 720
atcaaagtca atgactcagt gcccttccac ctgggctgga acagcacgga gcgctcgctg 780
cggcttcagg cgcgctacca cgacacgccc tacaagccac ccgccggccg cgccgcagcg 840
ccagagctga gctaccgagt gtgcgtgggc tcagacgtca cctccatcca caagtacatg 900
gtgcgtcgct acttcaacaa gccgtcaagg gtgccagcac ccgaggcctt ccgagacccc 960
atttggtcca catgggcgct gtacgggcgc gccgtggacc aggacaaggt gctgcgtttt 1020
gcccaacaga tccgcctgca ccacttcaac agcagccacc tggaaatcga cgacatgtac 1080
acacctgctt atggcgactt cgacttcgat gaggtcaaat tccccaacgc cagcgacatg 1140
ttccgccgcc tgcgcgacgc cggcttccgc gtcacgctct gggtgcaccc ttttgtcaac 1200
tacaactcgt cgcgcttcgg cgagggcgtg gagcgcgagc tgttcgtgcg cgaacccacg 1260
ggccggttac ctgcgctggt gcgctggtgg aacggcatcg gcgcggtgct agacttcacg 1320
cacccaaagg cccgcgactg gttccaggga cacctgcggc ggctgcgctc tcgctactcc 1380
gtggcttcct tcaagttcga cgcgggcgag gtcagctacc tgccgcggga cttcagcacc 1440
taccggccgc tgccggaccc cagcgtctgg agccggcgct acactgagat ggcgctgccc 1500
ttcttctcgc tggcggaggt gcgcgtaggc taccagtcac agaacatctc ctgcttcttc 1560
cgcctggtgg atcgcgactc tgtgtggggc tacgacctgg ggttgcgctc actcatcccc 1620
gcggtgctca ccgtcagcat gctgggctac ccattcatcc tacccgatat ggtgggcggc 1680
aacgccgtgc cccagcggac agccggcggc gatgtgcccg agcgcgagct ctacattcgc 1740
tggctggaag tggccgcctt tatgccggcc atgcagttct ctatcccgcc ctggcgctac 1800
gacgcggaag tggtggccat cgcgcagaag ttcgccgccc tgcgggcctc gcttgtggca 1860
ccgctgttgc ttgagctggc gggcgaggtc accgacacgg gtgaccctat cgtgcgcccc 1920
ctttggtgga ttgcgcccgg cgacgagaca gctcaccgta tcgactcgca gttccttatt 1980
ggggacacgc tgcttgtggc cccggtgctg gagccaggca agcaggagcg cgacgtctat 2040
ttgcccgccg gcaagtggcg cagctacaag ggtgagcttt tcgacaagac gccggtgctg 2100
ctcaccgatt acccggtcga cctggatgag atcgcctact ttacctgggc gtcctga 2157
<210> 10
<211> 2139
<212> DNA
<213> 智人(Homo sapiens)
<400> 10
atgctccaga accctcagga gaagagccag gcctaccccc gccgccgccg gcctggctgc 60
tacgcatacc gtcagaaccc cgaggccatc gcagccgcag ctatgtacac cttcctgccc 120
gacaacttct cacctgccaa gcccaagcct tccaaagacc tgaagccgct gctgggctcc 180
gcggttctgg ggctgctgct tgtgctggcc gcggtggtgg cctggtgcta ctacagcgtc 240
tccctacgca aggcggagcg acttcgcgcg gagctgctgg acctgaaagc tggcggcttc 300
tccatccgca atcagaaggg agagcaggtc ttccgcctgg ccttccgctc cggcgcgctg 360
gaccttgact cctgcagccg cgatggcgcc ctgctgggct gctcgctcac ggccgacggg 420
ctgccgctgc acttcttcat ccagactgtg cggcccaagg acacggtcat gtgctaccgc 480
gtgcgctggg aggaggcagc gccgggccgg gccgtggagc acgccatgtt cttgggcgac 540
gcggcggccc actggtatgg tggcgccgag atgaggacgc aacactggcc catccgcctg 600
gatggccagc aggagcccca gccgttcgtc accagcgatg tctactcctc cgacgccgcg 660
tttgggggca tcctcgagcg ctactggcta tcttcgcgcg cggccgccat caaagtcaat 720
gactcagtgc ccttccacct gggctggaac agcacggagc gctcgctgcg gcttcaggcg 780
cgctaccacg acacgcccta caagccaccc gccggccgcg ccgcagcgcc agagctgagc 840
taccgagtgt gcgtgggctc agacgtcacc tccatccaca agtacatggt gcgtcgctac 900
ttcaacaagc cgtcaagggt gccagcaccc gaggccttcc gagaccccat ttggtccaca 960
tgggcgctgt acgggcgcgc cgtggaccag gacaaggtgc tgcgttttgc ccaacagatc 1020
cgcctgcacc acttcaacag cagccacctg gaaatcgacg acatgtacac acctgcttat 1080
ggcgacttcg atgaggtcaa attccccaac gccagcgaca tgttccgccg cctgcgcgac 1140
gccggcttcc gcgtcacgct ctgggtgcac ccttttgtca actacaactc gtcgcgcttc 1200
ggcgagggcg tggagcgcga gctgttcgtg cgcgaaccca cgggccggtt acctgcgctg 1260
gtgcgctggt ggaacggcat cggcgcggtg ctagacttca cgcacccaaa ggcccgcgac 1320
tggttccagg gacacctgcg gcggctgcgc tctcgctact ccgtggcttc cttcaagttc 1380
gacgcgggcg aggtcagcta cctgccgcgg gacttcagca cctaccggcc gctgccggac 1440
cccagcgtct ggagccggcg ctacactgag atggcgctgc ccttcttctc gctggcggag 1500
gtgcgcgtag gctaccagtc acagaacatc tcctgcttct tccgcctggt ggatcgcgac 1560
tctgtgtggg gctacgacct ggggttgcgc tcactcatcc ccgcggtgct caccgtcagc 1620
atgctgggct acccattcat cctacccgat atggtgggcg gcaacgccgt gccccagcgg 1680
acagccggcg gcgatgtgcc cgagcgcgag ctctacattc gctggctgga agtggccgcc 1740
tttatgccgg ccatgcagtt ctctatcccg ccctggcgct acgacgcgga agtggtggcc 1800
atcgcgcaga agttcgccgc cctgcgggcc tcgcttgtgg caccgctgtt gcttgagctg 1860
gcgggcgagg tcaccgacac gggtgaccct atcgtgcgcc ccctttggtg gattgcgccc 1920
ggcgacgaga cagctcaccg tatcgactcg cagttcctta ttggggacac gctgcttgtg 1980
gccccggtgc tggagccagg caagcaggag cgcgacgtct atttgcccgc cggcaagtgg 2040
cgcagctaca agggtgagct tttcgacaag acgccggtgc tgctcaccga ttacccggtc 2100
gacctggatg agatcgccta ctttacctgg gcgtcctga 2139
<210> 11
<211> 714
<212> PRT
<213> 智人(Homo sapiens)
<400> 11
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Phe Asp Glu Val
355 360 365
Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly
370 375 380
Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser
385 390 395 400
Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr
405 410 415
Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val
420 425 430
Leu Asp Phe Thr His Pro Lys Ala Arg Asp Trp Phe Gln Gly His Leu
435 440 445
Arg Arg Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe Lys Phe Asp Ala
450 455 460
Gly Glu Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr Tyr Arg Pro Leu
465 470 475 480
Pro Asp Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu Met Ala Leu Pro
485 490 495
Phe Phe Ser Leu Ala Glu Val Arg Val Gly Tyr Gln Ser Gln Asn Ile
500 505 510
Ser Cys Phe Phe Arg Leu Val Asp Arg Asp Ser Val Trp Gly Tyr Asp
515 520 525
Leu Gly Leu Arg Ser Leu Ile Pro Ala Val Leu Thr Val Ser Met Leu
530 535 540
Gly Tyr Pro Phe Ile Leu Pro Asp Met Val Gly Gly Asn Ala Val Pro
545 550 555 560
Gln Arg Thr Ala Gly Gly Asp Val Pro Glu Arg Glu Leu Tyr Ile Arg
565 570 575
Trp Leu Glu Val Ala Ala Phe Met Pro Ala Met Gln Phe Ser Ile Pro
580 585 590
Pro Trp Arg Tyr Asp Ala Glu Val Val Ala Ile Ala Gln Lys Phe Ala
595 600 605
Ala Leu Arg Ala Ser Leu Val Ala Pro Leu Leu Leu Glu Leu Ala Gly
610 615 620
Glu Val Thr Asp Thr Gly Asp Pro Ile Val Arg Pro Leu Trp Trp Ile
625 630 635 640
Ala Pro Gly Asp Glu Thr Ala His Arg Ile Asp Ser Gln Phe Leu Ile
645 650 655
Gly Asp Thr Leu Leu Val Ala Pro Val Leu Glu Pro Gly Lys Gln Glu
660 665 670
Arg Asp Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser Tyr Lys Gly Glu
675 680 685
Leu Phe Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr Pro Val Asp Leu
690 695 700
Asp Glu Ile Ala Tyr Phe Thr Trp Ala Ser
705 710
<210> 12
<211> 74
<212> PRT
<213> 智人(Homo sapiens)
<400> 12
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala
65 70
<210> 13
<211> 442
<212> PRT
<213> 智人(Homo sapiens)
<400> 13
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Phe Asp Glu Val
355 360 365
Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly
370 375 380
Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser
385 390 395 400
Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr
405 410 415
Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val
420 425 430
Leu Asp Phe Thr His Pro Lys Ala Arg Asp
435 440
<210> 14
<211> 714
<212> PRT
<213> 智人(Homo sapiens)
<400> 14
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Val Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Phe Asp Glu Val
355 360 365
Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly
370 375 380
Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser
385 390 395 400
Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr
405 410 415
Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val
420 425 430
Leu Asp Phe Thr His Pro Lys Ala Arg Asp Trp Phe Gln Gly His Leu
435 440 445
Arg Arg Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe Lys Phe Asp Ala
450 455 460
Gly Glu Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr Tyr Arg Pro Leu
465 470 475 480
Pro Asp Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu Met Ala Leu Pro
485 490 495
Phe Phe Ser Leu Ala Glu Val Arg Val Gly Tyr Gln Ser Gln Asn Ile
500 505 510
Ser Cys Phe Phe Arg Leu Val Asp Arg Asp Ser Val Trp Gly Tyr Asp
515 520 525
Leu Gly Leu Arg Ser Leu Ile Pro Ala Val Leu Thr Val Ser Met Leu
530 535 540
Gly Tyr Pro Phe Ile Leu Pro Asp Met Val Gly Gly Asn Ala Val Pro
545 550 555 560
Gln Arg Thr Ala Gly Gly Asp Val Pro Glu Arg Glu Leu Tyr Ile Arg
565 570 575
Trp Leu Glu Val Ala Ala Phe Met Pro Ala Met Gln Phe Ser Ile Pro
580 585 590
Pro Trp Arg Tyr Asp Ala Glu Val Val Ala Ile Ala Gln Lys Phe Ala
595 600 605
Ala Leu Arg Ala Ser Leu Val Ala Pro Leu Leu Leu Glu Leu Ala Gly
610 615 620
Glu Val Thr Asp Thr Gly Asp Pro Ile Val Arg Pro Leu Trp Trp Ile
625 630 635 640
Ala Pro Gly Asp Glu Thr Ala His Arg Ile Asp Ser Gln Phe Leu Ile
645 650 655
Gly Asp Thr Leu Leu Val Ala Pro Val Leu Glu Pro Gly Lys Gln Glu
660 665 670
Arg Asp Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser Tyr Lys Gly Glu
675 680 685
Leu Phe Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr Pro Val Asp Leu
690 695 700
Asp Glu Ile Ala Tyr Phe Thr Trp Ala Ser
705 710
<210> 15
<211> 714
<212> PRT
<213> 智人(Homo sapiens)
<400> 15
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Phe Asp Glu Val
355 360 365
Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly
370 375 380
Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser
385 390 395 400
Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr
405 410 415
Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val
420 425 430
Leu Asp Phe Thr His Pro Lys Ala Gly Asp Trp Phe Gln Gly His Leu
435 440 445
Arg Arg Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe Lys Phe Asp Ala
450 455 460
Gly Glu Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr Tyr Arg Pro Leu
465 470 475 480
Pro Asp Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu Met Ala Leu Pro
485 490 495
Phe Phe Ser Leu Ala Glu Val Arg Val Gly Tyr Gln Ser Gln Asn Ile
500 505 510
Ser Cys Phe Phe Arg Leu Val Asp Arg Asp Ser Val Trp Gly Tyr Asp
515 520 525
Leu Gly Leu Arg Ser Leu Ile Pro Ala Val Leu Thr Val Ser Met Leu
530 535 540
Gly Tyr Pro Phe Ile Leu Pro Asp Met Val Gly Gly Asn Ala Val Pro
545 550 555 560
Gln Arg Thr Ala Gly Gly Asp Val Pro Glu Arg Glu Leu Tyr Ile Arg
565 570 575
Trp Leu Glu Val Ala Ala Phe Met Pro Ala Met Gln Phe Ser Ile Pro
580 585 590
Pro Trp Arg Tyr Asp Ala Glu Val Val Ala Ile Ala Gln Lys Phe Ala
595 600 605
Ala Leu Arg Ala Ser Leu Val Ala Pro Leu Leu Leu Glu Leu Ala Gly
610 615 620
Glu Val Thr Asp Thr Gly Asp Pro Ile Val Arg Pro Leu Trp Trp Ile
625 630 635 640
Ala Pro Gly Asp Glu Thr Ala His Arg Ile Asp Ser Gln Phe Leu Ile
645 650 655
Gly Asp Thr Leu Leu Val Ala Pro Val Leu Glu Pro Gly Lys Gln Glu
660 665 670
Arg Asp Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser Tyr Lys Gly Glu
675 680 685
Leu Phe Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr Pro Val Asp Leu
690 695 700
Asp Glu Ile Ala Tyr Phe Thr Trp Ala Ser
705 710
<210> 16
<211> 714
<212> PRT
<213> 智人(Homo sapiens)
<400> 16
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Leu Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Phe Asp Glu Val
355 360 365
Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly
370 375 380
Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser
385 390 395 400
Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr
405 410 415
Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val
420 425 430
Leu Asp Phe Thr His Pro Lys Ala Arg Asp Trp Phe Gln Gly His Leu
435 440 445
Arg Arg Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe Lys Phe Asp Ala
450 455 460
Gly Glu Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr Tyr Arg Pro Leu
465 470 475 480
Pro Asp Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu Met Ala Leu Pro
485 490 495
Phe Phe Ser Leu Ala Glu Val Arg Val Gly Tyr Gln Ser Gln Asn Ile
500 505 510
Ser Cys Phe Phe Arg Leu Val Asp Arg Asp Ser Val Trp Gly Tyr Asp
515 520 525
Leu Gly Leu Arg Ser Leu Ile Pro Ala Val Leu Thr Val Ser Met Leu
530 535 540
Gly Tyr Pro Phe Ile Leu Pro Asp Met Val Gly Gly Asn Ala Val Pro
545 550 555 560
Gln Arg Thr Ala Gly Gly Asp Val Pro Glu Arg Glu Leu Tyr Ile Arg
565 570 575
Trp Leu Glu Val Ala Ala Phe Met Pro Ala Met Gln Phe Ser Ile Pro
580 585 590
Pro Trp Arg Tyr Asp Ala Glu Val Val Ala Ile Ala Gln Lys Phe Ala
595 600 605
Ala Leu Arg Ala Ser Leu Val Ala Pro Leu Leu Leu Glu Leu Ala Gly
610 615 620
Glu Val Thr Asp Thr Gly Asp Pro Ile Val Arg Pro Leu Trp Trp Ile
625 630 635 640
Ala Pro Gly Asp Glu Thr Ala His Arg Ile Asp Ser Gln Phe Leu Ile
645 650 655
Gly Asp Thr Leu Leu Val Ala Pro Val Leu Glu Pro Gly Lys Gln Glu
660 665 670
Arg Asp Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser Tyr Lys Gly Glu
675 680 685
Leu Phe Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr Pro Val Asp Leu
690 695 700
Asp Glu Ile Ala Tyr Phe Thr Trp Ala Ser
705 710
<210> 17
<211> 202
<212> PRT
<213> 智人(Homo sapiens)
<400> 17
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly
195 200
<210> 18
<211> 714
<212> PRT
<213> 智人(Homo sapiens)
<400> 18
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Leu Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Phe Asp Glu Val
355 360 365
Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly
370 375 380
Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser
385 390 395 400
Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr
405 410 415
Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val
420 425 430
Leu Asp Phe Thr His Pro Lys Ala Arg Asp Trp Phe Gln Gly His Leu
435 440 445
Arg Arg Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe Lys Phe Asp Ala
450 455 460
Gly Glu Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr Tyr Arg Pro Leu
465 470 475 480
Pro Asp Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu Met Ala Leu Pro
485 490 495
Phe Phe Ser Leu Ala Glu Val Arg Val Gly Tyr Gln Ser Gln Asn Ile
500 505 510
Ser Cys Phe Phe Arg Leu Val Asp Arg Asp Ser Val Trp Gly Tyr Asp
515 520 525
Leu Gly Leu Arg Ser Leu Ile Pro Ala Val Leu Thr Val Ser Met Leu
530 535 540
Gly Tyr Pro Phe Ile Leu Pro Asp Met Val Gly Gly Asn Ala Val Pro
545 550 555 560
Gln Arg Thr Ala Gly Gly Asp Val Pro Glu Arg Glu Leu Tyr Ile Arg
565 570 575
Trp Leu Glu Val Ala Ala Phe Met Pro Ala Met Gln Phe Ser Ile Pro
580 585 590
Pro Trp Arg Tyr Asp Ala Glu Val Val Ala Ile Ala Gln Lys Phe Ala
595 600 605
Ala Leu Arg Ala Ser Leu Val Ala Pro Leu Leu Leu Glu Leu Ala Gly
610 615 620
Glu Val Thr Asp Thr Gly Asp Pro Ile Val Arg Pro Leu Trp Trp Ile
625 630 635 640
Ala Pro Gly Asp Glu Thr Ala His Arg Ile Asp Ser Gln Phe Leu Ile
645 650 655
Gly Asp Thr Leu Leu Val Ala Pro Val Leu Glu Pro Gly Lys Gln Glu
660 665 670
Arg Asp Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser Tyr Lys Gly Glu
675 680 685
Leu Phe Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr Pro Val Asp Leu
690 695 700
Asp Glu Ile Ala Tyr Phe Thr Trp Ala Ser
705 710
<210> 19
<211> 718
<212> PRT
<213> 智人(Homo sapiens)
<400> 19
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser Leu Ala Phe Arg
340 345 350
His Leu Glu Ile Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp
355 360 365
Phe Asp Glu Val Lys Phe Pro Asn Ala Ser Asp Met Phe Arg Arg Leu
370 375 380
Arg Asp Ala Gly Phe Arg Val Thr Leu Trp Val His Pro Phe Val Asn
385 390 395 400
Tyr Asn Ser Ser Arg Phe Gly Glu Gly Val Glu Arg Glu Leu Phe Val
405 410 415
Arg Glu Pro Thr Gly Arg Leu Pro Ala Leu Val Arg Trp Trp Asn Gly
420 425 430
Ile Gly Ala Val Leu Asp Phe Thr His Pro Lys Ala Arg Asp Trp Phe
435 440 445
Gln Gly His Leu Arg Arg Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe
450 455 460
Lys Phe Asp Ala Gly Glu Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr
465 470 475 480
Tyr Arg Pro Leu Pro Asp Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu
485 490 495
Met Ala Leu Pro Phe Phe Ser Leu Ala Glu Val Arg Val Gly Tyr Gln
500 505 510
Ser Gln Asn Ile Ser Cys Phe Phe Arg Leu Val Asp Arg Asp Ser Val
515 520 525
Trp Gly Tyr Asp Leu Gly Leu Arg Ser Leu Ile Pro Ala Val Leu Thr
530 535 540
Val Ser Met Leu Gly Tyr Pro Phe Ile Leu Pro Asp Met Val Gly Gly
545 550 555 560
Asn Ala Val Pro Gln Arg Thr Ala Gly Gly Asp Val Pro Glu Arg Glu
565 570 575
Leu Tyr Ile Arg Trp Leu Glu Val Ala Ala Phe Met Pro Ala Met Gln
580 585 590
Phe Ser Ile Pro Pro Trp Arg Tyr Asp Ala Glu Val Val Ala Ile Ala
595 600 605
Gln Lys Phe Ala Ala Leu Arg Ala Ser Leu Val Ala Pro Leu Leu Leu
610 615 620
Glu Leu Ala Gly Glu Val Thr Asp Thr Gly Asp Pro Ile Val Arg Pro
625 630 635 640
Leu Trp Trp Ile Ala Pro Gly Asp Glu Thr Ala His Arg Ile Asp Ser
645 650 655
Gln Phe Leu Ile Gly Asp Thr Leu Leu Val Ala Pro Val Leu Glu Pro
660 665 670
Gly Lys Gln Glu Arg Asp Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser
675 680 685
Tyr Lys Gly Glu Leu Phe Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr
690 695 700
Pro Val Asp Leu Asp Glu Ile Ala Tyr Phe Thr Trp Ala Ser
705 710 715
<210> 20
<211> 712
<212> PRT
<213> 智人(Homo sapiens)
<400> 20
Met Leu Gln Asn Pro Gln Glu Lys Ser Gln Ala Tyr Pro Arg Arg Arg
1 5 10 15
Arg Pro Gly Cys Tyr Ala Tyr Arg Gln Asn Pro Glu Ala Ile Ala Ala
20 25 30
Ala Ala Met Tyr Thr Phe Leu Pro Asp Asn Phe Ser Pro Ala Lys Pro
35 40 45
Lys Pro Ser Lys Asp Leu Lys Pro Leu Leu Gly Ser Ala Val Leu Gly
50 55 60
Leu Leu Leu Val Leu Ala Ala Val Val Ala Trp Cys Tyr Tyr Ser Val
65 70 75 80
Ser Leu Arg Lys Ala Glu Arg Leu Arg Ala Glu Leu Leu Asp Leu Lys
85 90 95
Ala Gly Gly Phe Ser Ile Arg Asn Gln Lys Gly Glu Gln Val Phe Arg
100 105 110
Leu Ala Phe Arg Ser Gly Ala Leu Asp Leu Asp Ser Cys Ser Arg Asp
115 120 125
Gly Ala Leu Leu Gly Cys Ser Leu Thr Ala Asp Gly Leu Pro Leu His
130 135 140
Phe Phe Ile Gln Thr Val Arg Pro Lys Asp Thr Val Met Cys Tyr Arg
145 150 155 160
Val Arg Trp Glu Glu Ala Ala Pro Gly Arg Ala Val Glu His Ala Met
165 170 175
Phe Leu Gly Asp Ala Ala Ala His Trp Tyr Gly Gly Ala Glu Met Arg
180 185 190
Thr Gln His Trp Pro Ile Arg Leu Asp Gly Gln Gln Glu Pro Gln Pro
195 200 205
Phe Val Thr Ser Asp Val Tyr Ser Ser Asp Ala Ala Phe Gly Gly Ile
210 215 220
Leu Glu Arg Tyr Trp Leu Ser Ser Arg Ala Ala Ala Ile Lys Val Asn
225 230 235 240
Asp Ser Val Pro Phe His Leu Gly Trp Asn Ser Thr Glu Arg Ser Leu
245 250 255
Arg Leu Gln Ala Arg Tyr His Asp Thr Pro Tyr Lys Pro Pro Ala Gly
260 265 270
Arg Ala Ala Ala Pro Glu Leu Ser Tyr Arg Val Cys Val Gly Ser Asp
275 280 285
Val Thr Ser Ile His Lys Tyr Met Val Arg Arg Tyr Phe Asn Lys Pro
290 295 300
Ser Arg Val Pro Ala Pro Glu Ala Phe Arg Asp Pro Ile Trp Ser Thr
305 310 315 320
Trp Ala Leu Tyr Gly Arg Ala Val Asp Gln Asp Lys Val Leu Arg Phe
325 330 335
Ala Gln Gln Ile Arg Leu His His Phe Asn Ser Ser His Leu Glu Ile
340 345 350
Asp Asp Met Tyr Thr Pro Ala Tyr Gly Asp Phe Asp Glu Val Lys Phe
355 360 365
Pro Asn Ala Ser Asp Met Phe Arg Arg Leu Arg Asp Ala Gly Phe Arg
370 375 380
Val Thr Leu Trp Val His Pro Phe Val Asn Tyr Asn Ser Ser Arg Phe
385 390 395 400
Gly Glu Gly Val Glu Arg Glu Leu Phe Val Arg Glu Pro Thr Gly Arg
405 410 415
Leu Pro Ala Leu Val Arg Trp Trp Asn Gly Ile Gly Ala Val Leu Asp
420 425 430
Phe Thr His Pro Lys Ala Arg Asp Trp Phe Gln Gly His Leu Arg Arg
435 440 445
Leu Arg Ser Arg Tyr Ser Val Ala Ser Phe Lys Phe Asp Ala Gly Glu
450 455 460
Val Ser Tyr Leu Pro Arg Asp Phe Ser Thr Tyr Arg Pro Leu Pro Asp
465 470 475 480
Pro Ser Val Trp Ser Arg Arg Tyr Thr Glu Met Ala Leu Pro Phe Phe
485 490 495
Ser Leu Ala Glu Val Arg Val Gly Tyr Gln Ser Gln Asn Ile Ser Cys
500 505 510
Phe Phe Arg Leu Val Asp Arg Asp Ser Val Trp Gly Tyr Asp Leu Gly
515 520 525
Leu Arg Ser Leu Ile Pro Ala Val Leu Thr Val Ser Met Leu Gly Tyr
530 535 540
Pro Phe Ile Leu Pro Asp Met Val Gly Gly Asn Ala Val Pro Gln Arg
545 550 555 560
Thr Ala Gly Gly Asp Val Pro Glu Arg Glu Leu Tyr Ile Arg Trp Leu
565 570 575
Glu Val Ala Ala Phe Met Pro Ala Met Gln Phe Ser Ile Pro Pro Trp
580 585 590
Arg Tyr Asp Ala Glu Val Val Ala Ile Ala Gln Lys Phe Ala Ala Leu
595 600 605
Arg Ala Ser Leu Val Ala Pro Leu Leu Leu Glu Leu Ala Gly Glu Val
610 615 620
Thr Asp Thr Gly Asp Pro Ile Val Arg Pro Leu Trp Trp Ile Ala Pro
625 630 635 640
Gly Asp Glu Thr Ala His Arg Ile Asp Ser Gln Phe Leu Ile Gly Asp
645 650 655
Thr Leu Leu Val Ala Pro Val Leu Glu Pro Gly Lys Gln Glu Arg Asp
660 665 670
Val Tyr Leu Pro Ala Gly Lys Trp Arg Ser Tyr Lys Gly Glu Leu Phe
675 680 685
Asp Lys Thr Pro Val Leu Leu Thr Asp Tyr Pro Val Asp Leu Asp Glu
690 695 700
Ile Ala Tyr Phe Thr Trp Ala Ser
705 710
<210> 21
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
ctcaggttcc agtattcatc 20
<210> 22
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
gtagcacatg accgtgtcct t 21
<210> 23
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
tccgcaatca gaagggagag 20
<210> 24
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
cgccgatgcc gttccaccag 20
<210> 25
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
cagccacctg gaaatcgacg acat 24
<210> 26
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
gcaggagatg ttctgtgact ggta 24
<210> 27
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
cgcgggactt cagcacctac 20
<210> 28
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
aagtcagcag ccacttaatg 20