CN108713642A - A kind of complex microorganism additive - Google Patents

A kind of complex microorganism additive Download PDF

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Publication number
CN108713642A
CN108713642A CN201810511770.0A CN201810511770A CN108713642A CN 108713642 A CN108713642 A CN 108713642A CN 201810511770 A CN201810511770 A CN 201810511770A CN 108713642 A CN108713642 A CN 108713642A
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freeze
dried powder
powder
hundred million
bacillus
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张奎昌
张婷婷
汤先伟
张路
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Pizhou Gas Star Pharmaceutical Technology Service Co Ltd
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Pizhou Gas Star Pharmaceutical Technology Service Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention discloses a kind of complex microorganism additive, it is characterized in that being uniformly mixed first by bacillus, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the charing egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made：Bacillus 5000 hundred million -30000 hundred million in every 1000g additives, lactic acid bacteria 5000 hundred million -30000 hundred million, 10000 hundred million -60000 hundred million, saccharomycete, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is charing egg-shell meal.Product content of the present invention is clear, application conducive to user and Quantitatively Selecting, using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and charing egg-shell meal as the matrix of additive, improve stability, trophism and the adsorptivity of additive institute mycetome, promote the nutritive value of product, promote the metabolism of animal, improve body resistance against diseases, promotes growth of animal.

Description

A kind of complex microorganism additive
Technical field
The invention belongs to feed additive fields, are related to a kind of microbial bacteria feed addition of the work for cultivated animals Agent.More particularly to a kind of complex microorganism additive.
Background technology
Probiotics is a kind of probiotics, also known as live bacteria agent or bacteria-promoting agent.This is a more comprehensive concept.It is Refer in animal body normal beneficial microbe through special process manufactured active bacteria formulation.The range of this concept includes micro- life Object growth promotion and probiotic, therewith it is similar be a more popular concept be microorganism fodder additive and one compared with For comprehensive concept.For microbial bacteria, one is microorganism growth promoters, then refer to the microorganism training for the work being added in feed Object is supported, effect is to participate in the balance of microorganism in enteron aisle, directly improves the efficiency of feed utilization and the speed of growth of animal.Such as gemma Bacillus class additive mainly generates enzyme, vitamin, amino acid and growth-stimulating factor etc. by living microorganism in enteron aisle, It directly reaches growth promotion and improves the purpose of efficiency of feed utilization.The other is probiotics, also referred to as regulator, are also added to feed In work culture of microorganism, it also assists in the balance of microorganism in enteron aisle, but effect is to increase micropopulation in animal intestine The inhibition fallen（I.e. dominant population acts on）, or by enhancing non-specific immune function prevent disease, and pick up To the effect for promoting growth and raising food utilization efficiency.If Bacillus acidi lactici class additive is not directly to play growth promoting function, But by improving body non-specific immune function, the enteron aisle sense with or without clinical symptom is reduced, to having grown indirectly product Pole acts on.Currently, there are many composite viable bacteria preparations in the market, both contains lactic acid bacteria, also there is bacillus, through Market Feedback, and Feeding effect is good.Market Feedback is more preferable by saccharomycete and Remixed agent effect made of lactic acid bacteria and bacillus, does not raise still It is also feeding therapeutic agent with nutritional agents and feed addictive, can also partly replaces treatment of the antibiotic for disease of digestive system. With regard to the dosage form that current microorganism fodder is used as additive, there are aqua and pulvis two major classes, aqueous solution dosage form to hold because of thalline Easily continuing to be metabolized using the nutritional substance in aqua, growth fermentation, therefore, the thalline of this dosage form plays pendulum, Storage life is shorter.Powder formulation is then that thalline or gemma are carried out to the thalline lived using drying means such as low temperature hot wind, freezings It is dehydrated at a slow speed, so that thalline or gemma is in stable drying dormant state, the substance that recycling can be feeding is as filler It is uniformly mixed and relatively stable powder-type biological feed additive is made.But with regard to current manufacturing enterprise being easy to get in view of raw material And processing cost, often select rice bran, wheat bran, corn flour, maize cob meal, peanut shell powder, oyster shell whiting, vermiculite power, mountain flour, carbonic acid One or more of calcium, medical stone, zeolite powder mixture, some also use plant straw powder or grass meal as filler material.This A bit in line with production cost the considerations of, then has ignored the stability of microbial bacteria and the trophism value of feed addictive.
In order in use, have certain adhesion, make in microbial bacteria additive microbe additive admix it is dynamic When being mixed in object feed, can be adsorbed on animal feed, convenient in animal feeding uniformity and be fed into the maximum of bacterium amount Change, some products take pains a certain amount of binder of addition.
In order to make the relative constant of raising animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thalline, meanwhile, these acidifying substances are to cultivation Animal also without the nutritive value directly utilized, meanwhile, there is prodigious irritation to the gastrointestinal tract of the cubs of livestock and poultry, young fowl, Compatibility is using not ideal enough.
Exploitation one kind can overcome above-mentioned deficiency, can either keep stability, the trophism of institute's mycetome, have again to thalline There is adhesion, while the filler to using has the microbe additive that nutritive value is provided to cultivated animals again, is to promote The basis of feed additive industry long-term stability development.
Invention content
The purpose of the present invention is to provide a kind of complex microorganism additive, which not only there is thalline to stablize, bacterium The body time-to-live is long, plays the Overlay of use in conjunction, and used auxiliary agents have certain adhesiveness and suction Attached property can make thalline preferably be bonding on one with auxiliary agents, meanwhile, complementary additive has certain acidity, right After animal feeding, abundant nutriment can not only be provided, while its acidic materials can keep stomach inner pH value relative constant, entered The effect of acidification is continued up to after small intestine.The purpose is to provide a kind of more complex microorganism of stability and trophism addition Agent.
A kind of complex microorganism additive of the present invention, it is characterized in that by bacillus, lactic acid bacteria, saccharomycete with it is a certain amount of Seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder, cactus powder and charing egg-shell meal uniformly mix, and obtain a kind of new compound micro- The thalline of bio-additive, this additive is stablized, and the thalline time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant Powder sugar content is high, and especially all there is cactus powder preferable Adhesion property, charing egg-shell meal also to have preferable adsorptivity.Its Middle sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, are not only provided to cultivated animals The nutriments such as abundant vitamin, the acidic materials after feeding play the effect for promoting digestion well, to highlight The purpose of the present invention.
In order to realize the present invention, the following technical solutions are specifically adopted：
A kind of complex microorganism additive, feature it be first by bacillus, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the charing egg-shell meal of surplus It is uniformly mixed, composite packaging is made：Bacillus 5000 hundred million -30000 hundred million, lactic acid bacteria 5000 in every 1000g additives A hundred million -30000 hundred million, 10000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 5-50g, saccharomycete, Rosa roxburghii freeze-dried powder 5-50g, wintercherry Freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is charing egg-shell meal.
Bacillus described above is bacillus dry powder product；The lactic acid bacteria is dry powder of lactic acid bacteria product；Institute The saccharomycete stated is saccharomycete dry powder product.
Bacillus dry powder product, dry powder of lactic acid bacteria product and the saccharomycete dry powder product, can use city respectively On sale is used as every gram of preparation that 200 hundred million are no less than containing viable bacteria concentration of feed addictive, the bacillus dry powder system Product, dry powder of lactic acid bacteria product and saccharomycete dry powder product and the weight proportion of the filler material in formula are produced by thalline dry powder and finally The thalline content of product determines.
Bacillus dry powder product, dry powder of lactic acid bacteria product and the saccharomycete dry powder product, can also utilize existing Then the culture medium that method choice accordingly optimizes uses high speed centrifugation mode by zymotic fluid respectively by liquid fermentation equipment culture In thalline separate, be placed in dry indoor cold wind or with vacuum freeze drying mode that bacterium mud is dry and be made, distinguish Obtain the bacillus thalline dry powder product of every gram of viable bacteria concentration no less than 200 hundred million, every gram of viable bacteria concentration no less than 200 hundred million Lactic acid bacteria thalline dry powder product, the saccharomycete thalline dry powder product that every gram of viable bacteria concentration is no less than 200 hundred million.
Bacillus described above is bacillus licheniformis, bacillus lentus, bacillus cereus, withered grass gemma Appointing in bacillus, bacillus coagulans, Japan bacillus, bacillus laterosporus, bacillus pumilus, bafillus natto Meaning is a kind of.
Saccharomycete described above is appointing in Candida, brewer's yeast, brewer's yeast, red yeast and fodder yeast Meaning is a kind of.
Lactic acid bacteria described above is lactobacillus lactis, streptococcus lactis, lactobacillus acidophilus, lactobacillus plantarum, acidified milk Bacillus, propionibacterium freudenreichii, Pediococcus acidilactici, Lactobacillus helveticus, Xie Shi Propionibacteriums, Pediococcus pentosaceus, does streptococcus thermophilus Appointing in junket lactic acid bacteria, lactobacillus plantarum, German-style lactobacillus, lactobacillus cellobiosas, lactobacillus bulgaricus and Bifidobacterium Meaning is a kind of.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, remove seed step；Rinsing condition described above is The citric acid of weight 0.1% is added with 40 DEG C of water, rinses 15min, and be stirred continuously, and is pulled out to be put into pure water and is rinsed 3 times；On It is that will be cooled to -45 DEG C or less rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively to state freezing；Above-mentioned distillation Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；It is above-mentioned Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, inner bag that polypropylene plastics pocket, outer packing is used to use aluminium Foil bag is filled with silica-gel desiccant between interior outer bag.
Charing egg-shell meal described above is made by following methods：
（1）Dry, dipping first cleans up eggshell, dries moisture, is dried 1-2 hours in advance, then pulverizes into 20- 30 mesh coarse powder, by weight 1:0.5 sprays the salt that prepared weight fraction is 25% on eggshell coarse powder（Sodium chloride）It is molten Liquid is mixed in sprinkling, is placed after mixing 6-7 hours, then uniformly mixing is primary again, then places 6-7 hours；The purpose is to allow Salt effectively penetrates into eggshell organization internal gap；
（2）The eggshell powder raw material impregnated is put into rotary carbonizing pot by charing, and charing is rotated at a temperature of 420-450 DEG C 70-80 minutes, until observing the complete blackening of raw material（Carbonization process should have corresponding scraping blade, to prevent raw material in carbonization process viscous Pot and caking）；
（3）The raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through carbon dioxide gas, is caught up with to the greatest extent wherein Air, at 720-800 DEG C anoxybiotic activate 55-85 minutes（Corresponding turning mechanism is set in activation furnace, keeps activation raw material every It can be stirred within 10 minutes once, to ensure activation fully and uniformly）；
（4）After activated material is taken out in cleaning, it is allowed to cool room temperature, activated material is gradually rinsed in rinse bath with clear water, until Until basic inspection does not measure sodium chloride；After having cleaned sodium chloride, it is small that the hydrochloric acid for being 12% with mass ratio submerges activated material 2.5-3 When, it waits after impregnating, with 95 DEG C of pure water washing activated materials, is floated after material grain is there is no white, then with room temperature pure water It is washed till neutrality（PH value is about in 6.9-7.1）, filter, obtain rinse activator material；
（5）Dry and screening, which dries the activated material of rinsed clean at 115-120 DEG C to water content, is less than 6%（w）, cross 60 mesh Sieve, the coarse grain after screening are pulverized again, make all to sieve to get charing egg-shell meal product by 60 mesh.
Drying described above uses heated-air drying or far-infrared ray drying；Dry condition is 180 DEG C -300 DEG C.It is dried Purpose be that can make the crisp matter of eggshell, increase eggshell hole be conducive to sprinkling saline solution effectively penetrate eggshell internal voids In.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell Close eggshell.
A kind of prioritization scheme of complex microorganism additive of the present invention is, first by bacillus, lactic acid bacteria, saccharomycete, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus 7000 hundred million -30000 in every 1000g additives Hundred million, lactic acid bacteria 7000 hundred million -30000 hundred million, saccharomycete is at 20000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii Tratt Freeze-dried powder 8-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g, remaining is charing Egg-shell meal.
The further prioritization scheme of the present invention is a kind of complex microorganism additive, it is characterized in that first by bacillus, Lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the mixing of cactus freeze-dried powder Uniformly, then add surplus charing egg-shell meal be uniformly mixed, composite packaging is made：The bacillus in every 1000g additives 9000 hundred million -30000 hundred million, lactic acid bacteria 9000 hundred million -30000 hundred million, 30000 hundred million -60000 hundred million, saccharomycete, sea-buckthorn jelly Dry powder 15-50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85- 150g, remaining is charing egg-shell meal.
The further prioritization scheme of the present invention is a kind of complex microorganism additive, it is characterized in that first by gemma bar Bacterium, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are mixed It closes uniform, then adds the charing egg-shell meal of surplus and be uniformly mixed, composite packaging is made：The bacillus in every 1000g additives 10000 hundred million -30000 hundred million, lactic acid bacteria 10000 hundred million -30000 hundred million, 40000 hundred million -60000 hundred million, saccharomycete, sea-buckthorn Freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-drying Powder 100-150g, remaining is charing egg-shell meal.
Bacillus mainly takes oxygen by force by biology in the gastrointestinal tract and maintains intestinal eubiosis, it is colonized in short-term in enteron aisle Afterwards, a large amount of oxygen can be consumed and maintain enteron aisle anaerobic environment, field planting power of the enhancing enteron aisle to anaerobic bacteria.In addition, bacillus It can be monosaccharide by Starch Conversion in enteron aisle, then these monosaccharide are converted to lactic acid by other strains in enteron aisle, reduce enteron aisle PH, to play the role of inhibit pathogen.
Probiotic composition made of bacillus and other microorganisms has a significant difference, bacillus can condition not Gemma is formed in the case of profit, will be protected self, and resurrection rate is high.Raw spore form exists within such product, have compared with More advantages：1. acidproof, salt tolerant, high temperature resistant（100℃）And anti-extrusion, thus the processing of feed granulation, pass through animal hydrochloric acid in gastric juice There is higher stability during environment etc.；2. the activity with very strong protease, lipase, amylase, to vegetalitas Carbohydrate has stronger degradation capability, can in degrading plant feed certain complexity compound, generate with antagonism The polypeptides matter of pathogenic entero becteria ability.This bacteroid has drop to the carbohydrate of many complexity of plant component part Solution ability, the substance that can be degraded have triglycerides, protein, starch, xylan, β-Isosorbide-5-Nitrae-galactan, pectin, carboxymethyl Cellulose, polygalacturonase, araban, glucose, p-nitrophenyl-glucopyranoside, p-nitrophenyl-Arab Furanoside, lichenin and p-nitrophenyl-furyl xylose etc..
The lactic acid bacteria being currently known has more than 100 kind.The applicating history of lactic acid bacteria is very long, such as dairy products, pickles, blueness Store the making and production of feed etc..There is a large amount of lactic acid bacterias in the enteron aisle of humans and animals.Lactobacillus preparation is that one kind contains The preparation of live lactobacillus, entitled alctasin.Lactic acid bacteria growth and development in enteron aisle decomposes carbohydrate and generates lactic acid and form acyclic acidic Border to inhibit the proliferation of harmful bacteria in intestinal canal, and prevents production malicious and pathogenic.The strain for preparing lactobacillus preparation has acidophilus newborn Bacillus, lactobacillus bulgaricus, streptococcus lactis and streptococcus fecalis etc..Leading at present to be applied has lactobacillus acidophilus, bifid breast bar Bacterium and enterococcus.
Lactobacillus is animal（Especially poultry）The normal settler of enteron aisle.Poultry went out after shell in a few hours can be The streptococcus fecalis, the enterobacteria that are dispersed in are found in enteron aisle（) and clostridium Enterobateria（Clostridia), with Bacillus acidi lactici just starts to settle down in alimentary canal in a couple of days afterwards.Lactobacillus is the main Pseudomonas of fowl crop flora, with large intestine Bacillus, streptococcus（Streptococci it) compares and occupies advantage.Bacillus acidi lactici is adhered to fowl crop epithelium, and this adhesion has kind Specificity.Fowl crop Bacillus acidi lactici is the Bacillus acidi lactici source that poultry maintains intestinal bacteria balance.Bacillus acidi lactici is maintaining to digest The aspect of stablizing of the normal microflora system in road plays conclusive effect.Most probiotic products be Lactobacillus or other Lactic acid-producing bacteria.
Lactic acid bacteria anaerobism or amphimicrobian growth, still are able to survive under the acid conditions of pH3.0 ~ 4.5.It is in animal intestinal tract Middle by monosaccharide, especially Lactose conversion is lactic acid, to reduce intestinal pH, prevents foreign bacteria in the field planting of enteron aisle, inhibits large intestine The growth of the pathogens such as bacillus, detection of Salmonella, and the growth of final lactic acid bacteria inhibiting itself.Lactic acid bacteria also can be by adjusting enteron aisle PH promotes gastrointestinal peristalsis to activate pepsin, helps the digestion of food, absorbs, mitigate flatulence and promote liver function etc.. Lactic acid bacteria is acted on by competitive exclusion（Competitive binding site）Inhibit the growth of pathogen.Lactic acid bacteria can also stimulate siberian crabapple System improves immunity of organisms, improves resistivity of the intestinal tissue to bacteria attack.Some Bacillus acidi lacticis can neutralize certain toxin, As lactobacillus bulgaricus can neutralize escherichia coli enterotoxin, but precise mechanism is unclear.Bacillus acidi lactici itself can also generate Some digestive ferments, such as invertase, lactase and peptase, help to improve animal digestion function.Sawada reports, certain lactic acid Contain a kind of safe and effective natural anti-hypertension compound SG-1 in bacilli-cell wall.Lang Zhongwu is equal to 1994 in egg young bird Brood of layers chichen is added in daily grain of chicken, as a result feed efficiency improves 8.73% ~ 11.23%；Xiao Zhenduo etc. (1995) is tried Verify bright, Brood of layers chichen is compared compared with antibiotic control group, improves feed efficiency 4.6%.In addition Bacillus acidi lactici can The digestive function of small intestine is improved by increasing surface area.Animal is in stress（As birth, wean, daily ration variation, intestinal pH are abnormal Increase) during, total variation tendency is that pathogen is increased, and Bacillus acidi lactici quantity is reduced.Lactobacillus is successfully established in enteron aisle PH can be made to become stable.
Saccharomycete (saccharomyce) is a kind of unicellular microorganism, and yeast cell wall main component is glucan, carefully Intracellular contains the multiple nutritional components such as abundant protein, nucleic acid, vitamin, carbohydrate, lipid material, minerals.Its The main nutrient composition of dry yeast is：Protein 40-50%, fat 1.5%, ash content 7%, crude fibre 1.5%, carbon hydrate Object 30-35%, moisture 5-7%.In addition, saccharomycete is also rich in B family vitamin, such as vitamin B1、B2、B6、B12, and wheat steroid The physiological activators such as alcohol, niacin, folic acid, pantothenic acid, inositol, the wherein content of wheat sterol and selenium are compared with horn of plenty.Except Yeast protein Other than matter, yeast metallothionein（MT）It is also a kind of low molecular weight, rich in cysteine, the metal that can be generated by metal inducement Binding protein.It is primarily involved in storage, transport and the metabolism of trace element, has and removes hydroxyl radical free radical, antagonism ionization and non-electrical From different physiological roles such as radiation and heavy metal detoxifications.
Yeast Glutathione peptide content is up to 1%, and the content of material is up to 8% in homoglutathion yeast.Glutathione is joined Contain Fe with internal redox reaction, activation SH enzymes and raising2+The effects that enzymatic activity is a kind of important biochemical drug.Paddy The sweet peptide of Guang can shorten dough kneading time and resting period；As antioxidant, the holding time of fried food can be extended, extracted Object is more preferable than glutathione effect is used alone；As feed addictive, animal's liver function is protected, improves fish growth, is increased Yield with milk.
B family vitamin content is up to 2% in yeast, and B family vitamin is also important biochemical drug, has a variety of important Biological function, such as glycometabolism, amino acid metabolism, transmethylation etc..
Also the ergosterol containing higher level in yeast, ergosterol is the precursor substance of vitamin D, in ultraviolet lighting It penetrates down and is converted to vitamin D.
Yeast cell wall polyoses content reaches 30%-35%, and there is growth promotion, antiviral, enhancing to be immunized, adjust the micro- life of enteron aisle State environment, the effect for promoting Development of Immune Organs.Existing literature report is by saccharomycete, lactic acid bacteria and bacillus compound in proportion Effect is more preferable afterwards, his not still feeding nutritional agents and feed addictive and feeding therapeutic agent, partly antibiotic can be replaced to be used for The treatment of disease of digestive system（Shi Bo is edited, novel fodder additive exploitation and application, Chemical Industry Press, in August, 2005 The first edition, page 121）.
Sea-buckthorn（Hippophae rhamnoides Linn.）, fruit contains abundant nutriment and biological active matter Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, aliphatic acid, trace element, sub- oil element, The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, every 100 grams In fruit juice, Vitamin C content can reach 825-1100 milligrams, be 2-3 times of Kiwi berry, be known as the laudatory title of the king of vitamin C.Contain Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt（Rosa roxbunghii）For the fruit of the perennial machaka rosa roxburghii of the rose family also known as mountain king fruit, thorn Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt contains abundant vitamin B, C, carrotene, The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, anorexia, indigestion or eating accumulation, glutted full bored, diarrhea Loose stool；Pyreticosis or hot summer weather hinder Tianjin, thirsty, scanty drak urine.
Ripe Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia Base acid, more than 10 the kinds trace element and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as Highest in preceding fruit, 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits is 50 times of citrus, Kiwi berry 10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat, Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry（Physali alkekengiL.）Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis Kind of nutritional ingredient, the content of wherein calcium is 73.1 times of tomato, 13.8 times of carrot, and ascorbic content is tomato 6.4 times, 5.4 times of carrot.With unique flavor and abundant nutrition.Fruit of Physalis aromatic flavour, delicious.Berry is rich Containing vitamin C, beta carotene, more than 20 kinds of mineral element and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect to be usually used in It controls in heat-syndrome cough, pharyngalgia, jaundice, dysentery, water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant（Ribes nigrum L.）Also known as currant, purple plum etc., ripening fruits are black Small berries, it is interior rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc Source；Ascorbic content is high, hence it is evident that higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, aldehydes matter.Seed Linoleic acid content is high.Ascorbic content is very abundant in blackcurrant, it is often more important that also contains a large amount of antioxygen and is melted into Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus（Scientific name：Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson）, It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch Shape, obovate elliptic or subcircular；Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling, The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point From extracting to obtain freidelin ketone（friedelin）, -3 α -ol of freidelin（friedelan-3-α-ol）, taraxerone （taraxerone）And taraxerol（taraxerol）.
Eggshell, what quantity was larger in China's poultry farming has chicken, duck, goose and quail etc., using egg, duck's egg, As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates, The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have Machine substance（Certainly, a large amount of remaining egg white in eggshell in the above ingredient are not included）.Eggshell mineral ingredient is mainly Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively 0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzyme, peptide matters can be measured, he Although content it is seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilization by eggshell in people's product, also have The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling makes an addition in feed The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is to clean eggshell, after high temperature drying, then be made one through spice, charing, activation, cleaning, drying, screening The adsorbent of kind function admirable, the program not only remains the nutrition that eggshell utilizes, while making the abundant carbonaceous of eggshell, not only right It is contained it is calcareous form organic calcium, and form the filling substrate with adsorption capacity.
The beneficial effects of the invention are as follows：
（1）Product of the present invention can highlight bacillus and lactic acid bacteria and the viable bacteria content of saccharomycete, be obviously improved gemma bar Bacterium, lactic acid bacteria, saccharomycete use in conjunction more preferably effect, significantly improve survival rate and stabilization of the thalline in closed composite packaging Property.
（2）Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and bud Spore bacillus, lactic acid bacteria and saccharomycete mixing, due to the viscosity in the colloidal substance that these dry powder formulations are rich in, especially cactus Substance can preferably increase the adhesive effect between additive and feed, and improve the utilization rate of additive；
（3）By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to improving animal immune energy Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion to contained complex microorganism Animal digestion all has significant effect；
（4）Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste Taste, free from extraneous odour add the preference of animal.
（5）Eggshell is first subjected to drying and other treatment through high temperature using charing egg-shell meal, further through spice, charing, activation, clear It washes, dry and sieves, form a kind of excellent adsorbent, which not only forms absorption to the saccharomycete in product Power, while making the eggshell after charing that still there is the organic calcium effect activated, meanwhile, after animal feeding, have well to stomach The toxin of enteron aisle has suction-operated, has stop dysentery, antidiarrheal effect for the animal of diarrhea and diarrhea, has good activated carbon Medicinal effects.
（6）To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, remove seed step Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
（7）The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus Powder, matrix of the charing egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix Element still contains abundant vitamin C, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol Substance, not only improves the nutritive value of feeding complex microorganism additive, while promoting the metabolism of animal, improves body Disease resistance promotes growth of animal, finally improves the economic benefit of its aquaculture.
（8）Product of the present invention uses interior outer bag composite packaging, interior outer bag interlayer to be filled with silica-gel desiccant, and product does not return Tide, no caking, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to its technical process for not having to state in detail in the present invention, be those skilled in the art common sense in Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention The construction of thousand Co., Ltds of Yao Tang traditional Chinese medical science research institute of cooperation unit Jiangsu has 20,000 tons of freezer facility, is to have to storage fresh fruit material It ensures enough.
Specific implementation mode
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder：
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively, remove carpopodium, and the impurity such as rotten fruit are heavy with containing 40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out It is drained；It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly Below；Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control- 22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%（w）, the time about needs 18 hours, is put into after taking-up In freezing crusher, control material temperature is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less It is spare in freezer.
Embodiment 2
Carbonize the preparation of egg-shell meal：
Eggshell is cleaned up first, dries moisture, is placed in electric baking case, the heated-air drying 2 hours under the conditions of 200 DEG C, After taking-up is let cool, 20 mesh coarse powder are pulverized into, by weight 1:0.5 sprays prepared weight ratio for 25% on eggshell coarse powder Salt solution（That is egg-shell meal：25% salt solution=1:0.5 ratio）, mixed in sprinkling, place 6 hours after mixing, then Uniformly mixing is primary again, then places 6 hours；The eggshell powder raw material impregnated is put into rotary carbonizing pot, in 420 DEG C of backspins Turn charing 80 minutes, until observing the complete blackening of raw material（Carbonization process prevents charcoal in being equipped with the scraping blade rotated automatically in charing pot Raw material glues pot and caking during change）；The raw material carbonized is rapidly moved in activation furnace, CO is passed through2Gas is caught up with therein to the greatest extent Air, anoxybiotic activates 80 minutes at 760 DEG C（Controllable stirring is set in activation furnace and stirs blade, is made every in activation raw material It is stirred within 10 minutes once, ensures activation material fully and uniformly）；After activation, activated material is taken out, is allowed to cool normal Temperature gradually rinses activated material in rinse bath with clear water, until basic inspection does not measure sodium chloride, has cleaned sodium chloride Afterwards, it with the hydrochloric acid of mass ratio 12% submergence activated material 2.5 hours, waits impregnating and finishes, with 95 DEG C of pure water washing activated materials, until After expecting grain there is no white, then with room temperature pure water rinse to pH value 7.0, filtering obtains rinse activator material；By rinsed clean Activated material, which is dried at 115 DEG C to water content, is less than 6%, crosses 60 mesh sieve, and the coarse grain after screening is pulverized again, makes all to pass through 60 mesh sieve is to get charing egg-shell meal product.
Embodiment 3
Carbonize the preparation of egg-shell meal：
Eggshell is cleaned up first, dries moisture, is placed in far-infrared dryer, it is 1 hour dry under the conditions of 300 DEG C, it takes Go out after letting cool, pulverize into 30 mesh coarse powder, by weight 1:0.5 sprays the food that prepared weight ratio is 25% on eggshell coarse powder Salting liquid（That is egg-shell meal：25% salt solution=1:0.5 ratio）, mixed in sprinkling, place 7 hours after mixing, then again Uniformly mixing is primary, then places 7 hours；The eggshell powder raw material impregnated is put into rotary carbonizing pot, is rotated at 450 DEG C Charing 70 minutes, until observing the complete blackening of raw material（Carbonization process prevents from carbonizing in being equipped with the scraping blade rotated automatically in charing pot Raw material glues pot and caking in the process）；The raw material carbonized is rapidly moved in activation furnace, CO is passed through2Gas catches up with sky therein to the greatest extent Gas, anoxybiotic activates 55 minutes at 800 DEG C（Controllable stirring is set in activation furnace and stirs blade, is made in activation raw material every 10 Minute is stirred once, ensures activation material fully and uniformly）；After activation, activated material is taken out, is allowed to cool normal Temperature gradually rinses activated material in rinse bath with clear water, until basic inspection does not measure sodium chloride, has cleaned sodium chloride Afterwards, it with the hydrochloric acid of mass ratio 12% submergence activated material 3 hours, waits impregnating and finishes, with 95 DEG C of pure water washing activated materials, until material After grain is there is no white, then with room temperature pure water rinse to pH value 7.0, filtering obtains rinse activator material；By the work of rinsed clean Material, which is dried at 120 DEG C to water content, is less than 6%, crosses 60 mesh sieve, and the coarse grain after screening is pulverized, made all by 60 again Mesh sieve is to get charing egg-shell meal product.
Embodiment 4
A kind of complex microorganism additive, be by bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus licheniformis 30000 hundred million, lactic acid in every 1000g additives 5000 hundred million, streptococcus, 40000 hundred million, red saccharomycete, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 50g, wintercherry freeze-dried powder 3g, Blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 50g, remaining is charing egg-shell meal；Specifically production method is：Embodiment 1 is weighed to divide Sea-buckthorn freeze-dried powder not obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, then by ground Clothing bacillus dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, and are then added surplus and are made by embodiment 2 After the charing egg-shell meal obtained is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics pocket, outer bag Using aluminium foil bag, a few packet silica-gel desiccants are added between interior outer bag, to prevent water suction.
Embodiment 5
A kind of complex microorganism additive, be by bacillus subtilis dry powder, lactobacillus fermenti dry powder, saccharomyces cerevisiae dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：The bacillus subtilis 10000 hundred million in every 1000g additives, fermentation Lactobacillus 10000 hundred million, saccharomyces cerevisiae 10000 hundred million, sea-buckthorn freeze-dried powder 45g, Rosa roxburghii freeze-dried powder 10g, wintercherry freeze-dried powder 5g, Blackcurrant freeze-dried powder 5g, cactus freeze-dried powder 100g, remaining is charing egg-shell meal；Specifically production method is：Embodiment 1 is weighed to divide Sea-buckthorn freeze-dried powder not obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, then by ground Clothing bacillus dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, and are then added surplus and are made by embodiment 2 After the charing egg-shell meal obtained is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics pocket, outer bag Using aluminium foil bag, a few packet silica-gel desiccants are added between interior outer bag, to prevent water suction.
Embodiment 6
A kind of complex microorganism additive, be by bacillus lentus dry powder, plant lactobacillus powder, brewer's yeast bacterium dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus lentus 9000 hundred million, plant in every 1000g additives Lactobacillus 7000 hundred million, brewer's yeast bacterium 60000 hundred million, sea-buckthorn freeze-dried powder 30g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 10g, Blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 65g, remaining is charing egg-shell meal；Specifically production method is：Embodiment 1 is weighed to divide Sea-buckthorn freeze-dried powder not obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, then by ground Clothing bacillus dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, and are then added surplus and are made by embodiment 2 After the charing egg-shell meal obtained is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics pocket, outer bag Using aluminium foil bag, a few packet silica-gel desiccants are added between interior outer bag, to prevent water suction.
Embodiment 7
A kind of complex microorganism additive is done by bacillus cereus dry powder, lactobacillus acidophilus dry powder, candida utili Powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The charing egg-shell meal of surplus is uniformly mixed, and composite packaging is made：The bacillus cereus 5000 hundred million in every 1000g additives, Lactobacillus acidophilus 20000 hundred million, candida utili 30000 hundred million, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, wintercherry are frozen Dry powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 100g, remaining is charing egg-shell meal；Specifically production method is：It weighs Sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are mixed respectively for embodiment 1 It is even, then bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, then add surplus by After the charing egg-shell meal obtained of embodiment 2 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Material bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 8
A kind of complex microorganism additive, be by bacillus pumilus dry powder, streptococcus thermophilus dried powder, saccharomyces cerevisiae dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus pumilus 30000 hundred million, thermophilic in every 1000g additives 30000 hundred million, streptococcus, saccharomyces cerevisiae 30000 hundred million, sea-buckthorn freeze-dried powder 30g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, cactus freeze-dried powder 110g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 3 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 9
A kind of complex microorganism additive, be by bafillus natto dry powder, Lactobacillus helveticus dry powder, red saccharomycete dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bafillus natto 5000 hundred million, Switzerland in every 1000g additives Lactobacillus 15000 hundred million, 50000 hundred million, red saccharomycete, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 120g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 3 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 10
A kind of complex microorganism additive, be by Japan's bacillus dry powder, Pediococcus acidilactici dry powder, fodder yeast bacterium dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Japan bacillus 20000 hundred million, lactic acid in every 1000g additives 30000 hundred million, piece coccus, fodder yeast bacterium 50000 hundred million, sea-buckthorn freeze-dried powder 30g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 30g, blackcurrant freeze-dried powder 30g, cactus freeze-dried powder 90g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 3 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 11
A kind of complex microorganism additive, be by bacillus laterosporus dry powder, lactobacillus plantarum dry powder, saccharomyces cerevisiae dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus laterosporus 25000 hundred million, plumule in every 1000g additives Lactobacillus 25000 hundred million, saccharomyces cerevisiae 50000 hundred million, sea-buckthorn freeze-dried powder 40g, Rosa roxburghii freeze-dried powder 40g, wintercherry freeze-dried powder 25g, blackcurrant freeze-dried powder 25g, cactus freeze-dried powder 100g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 3 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 12
A kind of complex microorganism additive is done by bacillus licheniformis dry powder, lactobacillus bulgaricus dry powder, fodder yeast bacterium Powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The charing egg-shell meal of surplus is uniformly mixed, and composite packaging is made：The bacillus licheniformis 30000 hundred million in every 1000g additives, Lactobacillus bulgaricus 30000 hundred million, fodder yeast bacterium 60000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, wintercherry Freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 80g, remaining is charing egg-shell meal；Specifically production method is：Claim The difference of Example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder Mixing, then bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, then add surplus After being sufficiently mixed uniformly by the charing egg-shell meal obtained of embodiment 3, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene Polybag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 13
A kind of complex microorganism additive is by bacillus subtilis dry powder, bifidobacteria dry powder, brewer's yeast bacterium dry powder, sand Spine freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus It carbonizes egg-shell meal to be uniformly mixed, composite packaging is made：Bacillus subtilis 15000 hundred million, bifid bar in every 1000g additives Bacterium 20000 hundred million, brewer's yeast bacterium 45000 hundred million are sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, wintercherry freeze-dried powder 15g, black Gallon freeze-dried powder 15g, cactus freeze-dried powder 60g, remaining is charing egg-shell meal；Specifically production method is：Weigh the difference of embodiment 1 Sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, then by lichens Bacillus dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, and are then added surplus and are made by embodiment 2 Charing egg-shell meal be sufficiently mixed uniformly after, carry out composite packaging；Composite packaging is that inner bag uses polypropylene plastics pocket, outer bag to adopt With aluminium foil bag, between interior outer bag plus a few packet silica-gel desiccants, to prevent water suction.
Embodiment 14
A kind of complex microorganism additive is by Japan's bacillus dry powder, lactobacillus acidophilus dry powder, Candida tropicalis Dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The charing egg-shell meal of surplus is added to be uniformly mixed, composite packaging is made：Japan bacillus 5000 in every 1000g additives Hundred million, lactobacillus acidophilus 5000 hundred million, Candida tropicalis 60000 hundred million, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 30g, acid Freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 80g are starched, remaining is charing egg-shell meal；Specifically production method is： Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying respectively Powder mixing, then bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, then more than addition After amount is sufficiently mixed uniformly by the charing egg-shell meal obtained of embodiment 2, composite packaging is carried out；Composite packaging is that inner bag uses poly- third Alkene plastics bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 15
A kind of complex microorganism additive, be by bafillus natto dry powder, lactobacillus fermenti dry powder, red saccharomycete dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：The bafillus natto 30000 hundred million in every 1000g additives, fermentation Lactobacillus 30000 hundred million, 10000 hundred million, red saccharomycete, sea-buckthorn freeze-dried powder 35g, Rosa roxburghii freeze-dried powder 40g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 120g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 3 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 16
A kind of complex microorganism additive, be by bacillus subtilis dry powder, streptococcus thermophilus dried powder, red saccharomycete dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus subtilis 15000 hundred million, thermophilic in every 1000g additives 15000 hundred million, streptococcus, 35000 hundred million, red saccharomycete, sea-buckthorn freeze-dried powder 45g, Rosa roxburghii freeze-dried powder 45g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 100g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 3 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 17
A kind of complex microorganism additive, be by bacillus cereus dry powder, Pediococcus acidilactici dry powder, brewer's yeast bacterium dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus cereus 6000 hundred million, lactic acid in every 1000g additives 6000 hundred million, piece coccus, brewer's yeast bacterium 55000 hundred million, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, wintercherry freeze-dried powder 15g, Blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 100g, remaining is charing egg-shell meal；Specifically production method is：Weigh embodiment 1 Sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing respectively, then will Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, and then add surplus by embodiment 3 After charing egg-shell meal obtained is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics pocket, outside Bag uses aluminium foil bag, adds a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 18
A kind of complex microorganism additive is by bacillus licheniformis dry powder, bifidobacteria dry powder, brewer's yeast bacterium dry powder, sand Spine freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus It carbonizes egg-shell meal to be uniformly mixed, composite packaging is made：Bacillus licheniformis 30000 hundred million, bifid bar in every 1000g additives Bacterium 30000 hundred million, brewer's yeast bacterium 50000 hundred million are sea-buckthorn freeze-dried powder 45g, Rosa roxburghii freeze-dried powder 45g, wintercherry freeze-dried powder 20g, black Gallon freeze-dried powder 20g, cactus freeze-dried powder 70g, remaining is charing egg-shell meal；Specifically production method is：Weigh the difference of embodiment 1 Sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, then by lichens Bacillus dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed, and are then added surplus and are made by embodiment 3 Charing egg-shell meal be sufficiently mixed uniformly after, carry out composite packaging；Composite packaging is that inner bag uses polypropylene plastics pocket, outer bag to adopt With aluminium foil bag, between interior outer bag plus a few packet silica-gel desiccants, to prevent water suction.
Embodiment 19
A kind of complex microorganism additive, be by bacillus licheniformis dry powder, streptococcus thermophilus dried powder, fodder yeast bacterium dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus licheniformis 25000 hundred million, thermophilic in every 1000g additives 25000 hundred million, streptococcus, fodder yeast bacterium 60000 hundred million, sea-buckthorn freeze-dried powder 45g, Rosa roxburghii freeze-dried powder 45g, wintercherry freeze-dried powder 18g, blackcurrant freeze-dried powder 18g, cactus freeze-dried powder 85g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 2 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.
Embodiment 20
A kind of complex microorganism additive, be by bacillus subtilis dry powder, plant lactobacillus powder, fodder yeast bacterium dry powder, Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus Charing egg-shell meal be uniformly mixed, composite packaging is made：Bacillus subtilis 30000 hundred million, plant in every 1000g additives Lactobacillus 30000 hundred million, fodder yeast bacterium 30000 hundred million, sea-buckthorn freeze-dried powder 35g, Rosa roxburghii freeze-dried powder 35g, wintercherry freeze-dried powder 25g, blackcurrant freeze-dried powder 25g, cactus freeze-dried powder 150g, remaining is charing egg-shell meal；Specifically production method is：Weigh implementation The difference of example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, Bacillus licheniformis dry powder, streptococcus lactis dry powder, red saccharomycete dry powder are uniformly admixed again, then add surplus by implementing After the charing egg-shell meal obtained of example 2 is sufficiently mixed uniformly, composite packaging is carried out；Composite packaging is that inner bag uses polypropylene plastics Bag, outer bag use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, to prevent water suction.

Claims (12)

1. a kind of complex microorganism additive, feature it be first by bacillus, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the charing eggshell of surplus Powder is uniformly mixed, and composite packaging is made：Bacillus 5000 hundred million -30000 hundred million, lactic acid bacteria in every 1000g additives 5000 hundred million -30000 hundred million, 10000 hundred million -60000 hundred million, saccharomycete, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, Wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is charing egg-shell meal；Described Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made by following methods, Technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, and the impurity such as rotten fruit rinse, is cold Jelly, lyophilization, low-temperature grinding are not removed the peel, remove seed step；Rinsing condition described above is to add weight with 40 DEG C of water 0.1% citric acid rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing is respectively will be husky It is cooled to -45 DEG C or less rapidly after spine, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot；The process conditions of above-mentioned lyophilization are Temperature-22-- 32 DEG C, vacuum degree 120-150Pa are dry until moisture is less than 6%/w；The charing egg-shell meal be by Following methods are made：（1）Dry, dipping first cleans up eggshell, dries moisture, in advance drying process 1-2 hours, then 20-30 mesh coarse powder is pulverized into, by weight 1:0.5 sprays the salt that prepared weight fraction is 25% on eggshell coarse powder （Sodium chloride）Solution is mixed in sprinkling, is placed after mixing 6-7 hours, then uniformly mixing is primary again, then places 6-7 hours； The purpose is to allow salt effectively to penetrate into eggshell organization internal gap；（2）The eggshell powder raw material impregnated is put by charing In rotary carbonizing pot, rotation charing 70-80 minutes at a temperature of 420-450 DEG C, until observing the complete blackening of raw material；（3） The raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through carbon dioxide gas, catches up with air therein to the greatest extent, Anoxybiotic activates 55-85 minutes at 720-800 DEG C；（4）After activated material is taken out in cleaning, it is allowed to cool room temperature, activated material is existed It is gradually rinsed with clear water in rinse bath, until not measuring sodium chloride until examining substantially；After having cleaned sodium chloride, it is with mass ratio 12% hydrochloric acid submergence activated material 2.5-3 hour, is waited after impregnating, with 95 DEG C of pure water washing activated materials, until expecting grain not After having white again, then with room temperature pure water rinse to neutrality, filtering obtains rinse activator material；（5）It dries and sieves rinsed clean Activated material dried to water content at 115-120 DEG C and be less than 6%/w, cross 60 mesh sieve, the coarse grain after screening pulverized, made again All by 60 mesh sieve to get.
2. a kind of complex microorganism additive, feature it be first by bacillus, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the charing eggshell of surplus Powder is uniformly mixed, and composite packaging is made：Bacillus 7000 hundred million -30000 hundred million, lactic acid bacteria in every 1000g additives 7000 hundred million -30000 hundred million, saccharomycete is at 20000 hundred million -60000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 8- 50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70-150g, remaining is charing egg-shell meal；Institute Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the cactus freeze-dried powder stated, by following methods system , technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium, the impurity such as rotten fruit, drift respectively Wash, freeze, lyophilization, low-temperature grinding, do not remove the peel, remove seed step；Rinsing condition described above is added with 40 DEG C of water The citric acid of weight 0.1% rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing is difference It will be cooled to -45 DEG C or less rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot；The technique item of above-mentioned lyophilization Part is temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；The charing egg-shell meal It is to be made by following methods：（1）Dry, dipping first cleans up eggshell, dries moisture, is dried 1-2 hours in advance, Then 20-30 mesh coarse powder is pulverized into, by weight 1:0.5 sprays the food that prepared weight fraction is 25% on eggshell coarse powder Salt（Sodium chloride）Solution mix in sprinkling, places 6-7 hour after mixing, then uniform again to mix once, then to place 6-7 small When；The purpose is to allow salt effectively to penetrate into eggshell organization internal gap；（2）The eggshell powder raw material impregnated is put in charing Enter in rotary carbonizing pot, rotation charing 70-80 minutes at a temperature of 420-450 DEG C, until observing the complete blackening of raw material； （3）The raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through carbon dioxide gas, catches up with sky therein to the greatest extent Gas, anoxybiotic activates 55-85 minutes at 720-800 DEG C；（4）After activated material is taken out in cleaning, it is allowed to cool room temperature, by activated material It is gradually rinsed with clear water in rinse bath, until not measuring sodium chloride until examining substantially；After having cleaned sodium chloride, it is with mass ratio 12% hydrochloric acid submergence activated material 2.5-3 hour, is waited after impregnating, with 95 DEG C of pure water washing activated materials, until expecting grain not After having white again, then with room temperature pure water rinse to neutrality, filtering obtains rinse activator material；（5）It dries and sieves rinsed clean Activated material dried to water content at 115-120 DEG C and be less than 6%/w, cross 60 mesh sieve, the coarse grain after screening pulverized, made again All by 60 mesh sieve to get.
3. a kind of complex microorganism additive, feature it be first by bacillus, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the charing eggshell of surplus Powder is uniformly mixed, and composite packaging is made：Bacillus 9000 hundred million -30000 hundred million, lactic acid bacteria in every 1000g additives 9000 hundred million -30000 hundred million, 30000 hundred million -60000 hundred million, saccharomycete, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 12- 50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g, remaining is charing egg-shell meal；Institute Sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the cactus freeze-dried powder stated, by following methods system , technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium, the impurity such as rotten fruit, drift respectively Wash, freeze, lyophilization, low-temperature grinding, do not remove the peel, remove seed step；Rinsing condition described above is added with 40 DEG C of water The citric acid of weight 0.1% rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing is difference It will be cooled to -45 DEG C or less rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot；The technique item of above-mentioned lyophilization Part is temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；The charing egg-shell meal It is to be made by following methods：（1）Dry, dipping first cleans up eggshell, dries moisture, is dried 1-2 hours in advance, Then 20-30 mesh coarse powder is pulverized into, by weight 1:0.5 sprays the food that prepared weight fraction is 25% on eggshell coarse powder Salt（Sodium chloride）Solution mix in sprinkling, places 6-7 hour after mixing, then uniform again to mix once, then to place 6-7 small When；The purpose is to allow salt effectively to penetrate into eggshell organization internal gap；（2）The eggshell powder raw material impregnated is put in charing Enter in rotary carbonizing pot, rotation charing 70-80 minutes at a temperature of 420-450 DEG C, until observing the complete blackening of raw material； （3）The raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through carbon dioxide gas, catches up with sky therein to the greatest extent Gas, anoxybiotic activates 55-85 minutes at 720-800 DEG C；（4）After activated material is taken out in cleaning, it is allowed to cool room temperature, by activated material It is gradually rinsed with clear water in rinse bath, until not measuring sodium chloride until examining substantially；After having cleaned sodium chloride, it is with mass ratio 12% hydrochloric acid submergence activated material 2.5-3 hour, is waited after impregnating, with 95 DEG C of pure water washing activated materials, until expecting grain not After having white again, then with room temperature pure water rinse to neutrality, filtering obtains rinse activator material；（5）It dries and sieves rinsed clean Activated material dried to water content at 115-120 DEG C and be less than 6%/w, cross 60 mesh sieve, the coarse grain after screening pulverized, made again All by 60 mesh sieve to get.
4. a kind of complex microorganism additive, feature it be first by bacillus, lactic acid bacteria, saccharomycete, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the charing eggshell of surplus Powder is uniformly mixed, and composite packaging is made：Bacillus 10000 hundred million -30000 hundred million, lactic acid bacteria in every 1000g additives 10000 hundred million -30000 hundred million, 40000 hundred million -60000 hundred million, saccharomycete, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 15- 50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100-150g, remaining is charing eggshell Powder；The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, by with lower section Method is made, and technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, and rotten fruit etc. is miscellaneous Matter, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, remove seed step；Rinsing condition described above is with 40 DEG C of water The citric acid of weight 0.1% is added, 15min is rinsed, and is stirred continuously, pulls out to be put into pure water and rinses 3 times；Above-mentioned freezing is It will be cooled to -45 DEG C or less rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively；The work of above-mentioned lyophilization Skill condition is temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w；The charing egg Shell powder is made by following methods：（1）Dry, dipping first cleans up eggshell, dries moisture, is dried 1-2 in advance Hour, 20-30 mesh coarse powder is then pulverized into, by weight 1:0.5 sprays prepared weight fraction on eggshell coarse powder is 25% salt（Sodium chloride）Solution is mixed in sprinkling, is placed after mixing 6-7 hours, then uniformly mixing is primary again, then puts It sets 6-7 hours；The purpose is to allow salt effectively to penetrate into eggshell organization internal gap；（2）Carbonize the eggshell that will have been impregnated Powder raw material is put into rotary carbonizing pot, and rotation charing 70-80 minutes at a temperature of 420-450 DEG C, observation raw material becomes completely Until black；（3）The raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through carbon dioxide gas, is caught up with and is use up it In air, at 720-800 DEG C anoxybiotic activate 55-85 minutes；（4）After activated material is taken out in cleaning, it is allowed to cool room temperature, it will Activated material is gradually rinsed in rinse bath with clear water, until not measuring sodium chloride until examining substantially；After having cleaned sodium chloride, matter is used Amount waits after impregnating than submerging activated material 2.5-3 hours for 12% hydrochloric acid, with 95 DEG C of pure water washing activated materials, until Expect grain there is no white after, then use room temperature pure water rinse to neutrality, filter, obtain rinse activator material；（5）Dry and screening will drift The activated material of wash clean, which is dried at 115-120 DEG C to water content, is less than 6%/w, crosses 60 mesh sieve, and the coarse grain after screening carries out again Pulverize, make all by 60 mesh sieve to get.
5. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the bacillus, lactic acid Bacterium and saccharomycete, the respectively every gram thalline dry powder product that 200 hundred million are no less than containing viable bacteria concentration.
6. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the bacillus is lichens Bacillus, bacillus lentus, bacillus cereus, bacillus subtilis, bacillus coagulans, Japan bacillus, side Any one in spore bacillus, bacillus pumilus, bafillus natto.
7. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the lactic acid bacteria is lactic acid breast Bacillus, streptococcus lactis, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus fermenti, streptococcus thermophilus, propionibacterium freudenreichii, lactic acid Piece coccus, Lactobacillus helveticus, Xie Shi Propionibacteriums, Pediococcus pentosaceus, cheese lactic acid bacteria, lactobacillus plantarum, German-style lactobacillus, fibre Tie up any one in disaccharides lactobacillus, lactobacillus bulgaricus and Bifidobacterium.
8. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the saccharomycete is false silk ferment Any one in mother, brewer's yeast, brewer's yeast, red yeast and fodder yeast.
9. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the low-temperature grinding is material Temperature is crushed in 0 DEG C or less.
10. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the composite packaging uses Interior outer bag composite packaging, inner bag use polypropylene plastics pocket, outer packing to use aluminium foil bag, silica dehydrator is filled between interior outer bag Agent pouch.
11. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the eggshell be egg shell, Any one or a few mixing eggshell in Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
12. a kind of complex microorganism additive as described in claim 1-4, which is characterized in that the drying uses hot wind Dry or far-infrared ray drying；The drying condition is 180 DEG C -300 DEG C.
CN201810511770.0A 2018-05-25 2018-05-25 A kind of complex microorganism additive Withdrawn CN108713642A (en)

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